Rw4G016180

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002711
Physical Location & Seq
Forward (+)
37142900 .. 37145346
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016180.1

Sequence Viewer

Length: 1287 bp
ATGGACTCAAACTCTTCAACAATGTCTCATGTTGCTACTATTGTTATACTGGGATTACTATTCCTACAAGCGGCAGAATGTAGAATACAGAATATTAAGGATGTAGTTACGTACTCCGCCATTAACTGCCGGAAGCACAGTGCTCTTTTGACTGATTTCGGAGCTCTAATTGTGCCCCCCGGTAACTGGCTTACCGGGAGCTTCAACCTCACTAGCCACTTTACCCTTTTCCTCCACAAGGATGCTGTTATTCTTGGGTCTCAGGATGAGTCCGAGTGGCCTCTTCTTCCAGCACTGCCTTCATACGGGCAAGGGAGAGAGGGCCCTGGTGGAAGGTTAAGCAGTCTGATCACTGGAACAAACCTTACCGACGTCGTAATTACCGGTAACAATGGGACCATTGATGGGCAAGGCGCTCCCTGGTGGGAAAAATTCAAGAAGGGTCTTTTGAATGTCACCCGACCATACTTGGTTGAGATTATGTACTCTAATGAGATCCAAATCTCCAATCTCACTTTTACGAATTCTCCATCCTGGCACATCCATCCCATTTACAGCAGTAACATTACCATACAAGGCCTCACTATCCTTGCACCAGTTAGCGTTCCTAACACAGATGGGATTAACCCTGATTCGTTCTCCGGGGATGACTGCATTGCAGTGAAGAGTGGCTGGGATCAGTATGGAATCAAATTTGGAATGCCAACCGAGCATCTTGTTATCAGGAGGCTTACCTGTATTTCACCTAAAAGTGCTACTGTTGCCCTAGGCAGTGAAATGTCTGGTGGAATCAGGGATGTAAGAGTTGAAGATGTCACAGCCATTCACACCGAATCCAGCATTAGGATCAAAACAGCTGTAGGCAGAGGGGCTTATGTCAAAGACATTTATGTACGGAGATTGAAGTTGAAGACTATGAAATACGTTTTTTGGATGACAGGCGGCTATGGTTCACATCCAGGCGAGGGCTTTGATCCAAAGGCACTTCCTGAGATTCAAAACATCAACTATAGAGAGGTTGTTGCTGAGAATGTTACAATGCCTGCCCAGTTGGATGGAATTGCTAGTGATCCTTTCAAGGGAATTTGCATCTCTAATGTGAGTATCTCACTCACTGAAAAGCCAAAGAAATTGTTGTGGAACTGTACTGATGTTGAGGGCATTACGAGCAATGTGACTCCAAAGGCTTGCGATTTGTTGCCCGAGCAGCAAAAACCAATCGATTGTGCTTTCCCTGAGGATAAGTTAGCCATTGAGGGCGTTAAGCTGACCACTTGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

428

Amino Acids

46.63

Weight (kDa)

6.01

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 115 - 386 2.7e-26 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 375
AciI CCGC 3 cut(s) 71, 117, 942
AclWI GGATC 5 cut(s) 490, 684, 854, 968, 1064
AcsI RAATTY 4 cut(s) 431, 523, 692, 1083
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 4 cut(s) 113, 485, 894, 1147
AfiI CCNNNNNNNGG 8 cut(s) 70, 186, 238, 305, 405, 843, 965, 1079
AgeI ACCGGT 1 cut(s) 383
AgsI TTSAA 9 cut(s) 18, 205, 436, 451, 809, 904, 910, 998, 1078
AjnI CCWGG 4 cut(s) 325, 419, 533, 958
AluBI AGCT 4 cut(s) 164, 201, 857, 1267
AluI AGCT 4 cut(s) 164, 201, 857, 1267
Alw21I GWGCWC 2 cut(s) 145, 166
Alw26I GTCTC 2 cut(s) 30, 264
AlwI GGATC 5 cut(s) 490, 684, 854, 968, 1064
Ama87I CYCGRG 1 cut(s) 1202
AoxI GGCC 3 cut(s) 278, 322, 577
ApaI GGGCCC 1 cut(s) 326
ApeKI GCWGC 1 cut(s) 1207
ApoI RAATTY 4 cut(s) 431, 523, 692, 1083
AsiGI ACCGGT 1 cut(s) 383
AspA2I CCTAGG 1 cut(s) 766
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 3 cut(s) 322, 323, 396
AsuC2I CCSGG 3 cut(s) 180, 196, 643
AsuHPI GGTGA 2 cut(s) 448, 735
AvaI CYCGRG 1 cut(s) 1202
AvaII GGWCC 1 cut(s) 396
AvrII CCTAGG 1 cut(s) 766
AxyI CCTNAGG 1 cut(s) 1236
BaeGI GKGCMC 2 cut(s) 177, 326
BanII GRGCYC 2 cut(s) 166, 326
BbsI GAAGAC 1 cut(s) 917
Bbv12I GWGCWC 2 cut(s) 145, 166
BbvI GCAGC 1 cut(s) 1219
BccI CCATC 5 cut(s) 398, 538, 552, 611, 1049
BciT130I CCWGG 4 cut(s) 327, 421, 535, 960
BclI TGATCA 1 cut(s) 348
BcnI CCSGG 3 cut(s) 180, 196, 643
BcoDI GTCTC 2 cut(s) 30, 264
BfaI CTAG 3 cut(s) 213, 767, 1065
BfmI CTRYAG 2 cut(s) 858, 1009
BfoI RGCGCY 1 cut(s) 417
BisI GCNGC 3 cut(s) 72, 943, 1208
BlnI CCTAGG 1 cut(s) 766
BlsI GCNGC 3 cut(s) 73, 944, 1209
Bme1390I CCNGG 7 cut(s) 180, 196, 327, 421, 535, 643, 960
Bme18I GGWCC 1 cut(s) 396
BmeT110I CYCGRG 1 cut(s) 1202
BmgT120I GGNCC 3 cut(s) 322, 323, 396
BmiI GGNNCC 2 cut(s) 324, 397
BmrFI CCNGG 7 cut(s) 180, 196, 327, 421, 535, 643, 960
BmrI ACTGGG 2 cut(s) 59, 1042
BmsI GCATC 3 cut(s) 232, 721, 1098
BmuI ACTGGG 2 cut(s) 59, 1042
BpiI GAAGAC 1 cut(s) 917
BpuMI CCSGG 3 cut(s) 180, 196, 643
Bsa29I ATCGAT 1 cut(s) 1221
BsaAI YACGTR 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 500
BsaHI GRCGYC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 5 cut(s) 178, 325, 419, 642, 766
BsaWI WCCGGW 1 cut(s) 383
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 186, 238, 305, 405, 843, 965, 1079
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 5 cut(s) 54, 191, 358, 596, 1048
Bse21I CCTNAGG 1 cut(s) 1236
Bse3DI GCAATG 2 cut(s) 654, 1177
Bse8I GATNNNNATC 1 cut(s) 500
BseBI CCWGG 4 cut(s) 327, 421, 535, 960
BseCI ATCGAT 1 cut(s) 1221
BseDI CCNNGG 5 cut(s) 178, 325, 419, 642, 766
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 8 cut(s) 70, 186, 238, 305, 405, 843, 965, 1079
BseMI GCAATG 2 cut(s) 654, 1177
BseMII CTCAG 4 cut(s) 275, 981, 1017, 1227
BseNI ACTGG 5 cut(s) 54, 191, 358, 596, 1048
BseSI GKGCMC 2 cut(s) 177, 326
BseXI GCAGC 1 cut(s) 1219
BseYI CCCAGC 1 cut(s) 672
BshFI GGCC 3 cut(s) 280, 324, 579
BshTI ACCGGT 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 1221
BsiHKAI GWGCWC 2 cut(s) 145, 166
BsiHKCI CYCGRG 1 cut(s) 1202
BsiSI CCGG 5 cut(s) 130, 180, 195, 384, 642
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 8 cut(s) 70, 186, 238, 305, 405, 843, 965, 1079
BsmAI GTCTC 2 cut(s) 30, 264
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 1 cut(s) 705
BsnI GGCC 3 cut(s) 280, 324, 579
Bso31I GGTCTC 1 cut(s) 264
BsoBI CYCGRG 1 cut(s) 1202
Bsp120I GGGCCC 1 cut(s) 322
Bsp1286I GDGCHC 4 cut(s) 145, 166, 177, 326
Bsp143I GATC 6 cut(s) 348, 495, 676, 846, 973, 1069
BspACI CCGC 3 cut(s) 71, 117, 942
BspANI GGCC 3 cut(s) 280, 324, 579
BspCNI CTCAG 4 cut(s) 274, 982, 1018, 1228
BspDI ATCGAT 1 cut(s) 1221
BspLI GGNNCC 2 cut(s) 324, 397
BspPI GGATC 5 cut(s) 490, 684, 854, 968, 1064
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 2 cut(s) 654, 1177
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 5 cut(s) 54, 191, 358, 596, 1048
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 5 cut(s) 178, 325, 419, 642, 766
BssMI GATC 6 cut(s) 348, 495, 676, 846, 973, 1069
BssNI GRCGYC 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 766
Bst2UI CCWGG 4 cut(s) 327, 421, 535, 960
Bst4CI ACNGT 3 cut(s) 140, 760, 1145
Bst6I CTCTTC 3 cut(s) 19, 288, 659
BstACI GRCGYC 1 cut(s) 372
BstBAI YACGTR 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 1044, 1189
BstDEI CTNAG 4 cut(s) 261, 990, 1026, 1236
BstENI CCTNNNNNAGG 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 6 cut(s) 351, 498, 679, 849, 976, 1072
BstMAI GTCTC 2 cut(s) 30, 264
BstMBI GATC 6 cut(s) 348, 495, 676, 846, 973, 1069
BstMWI GCNNNNNNNGC 4 cut(s) 709, 761, 1167, 1207
BstNI CCWGG 4 cut(s) 327, 421, 535, 960
BstSCI CCNGG 7 cut(s) 178, 194, 325, 419, 533, 641, 958
BstSFI CTRYAG 2 cut(s) 858, 1009
BstSLI GKGCMC 2 cut(s) 177, 326
BstSNI TACGTA 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 1219
BstV2I GAAGAC 1 cut(s) 917
BstX2I RGATCY 1 cut(s) 495
BstXI CCANNNNNNTGG 1 cut(s) 1055
BstYI RGATCY 1 cut(s) 495
Bsu15I ATCGAT 1 cut(s) 1221
Bsu36I CCTNAGG 1 cut(s) 1236
BsuRI GGCC 3 cut(s) 280, 324, 579
BsuTUI ATCGAT 1 cut(s) 1221
BtsI GCAGTG 3 cut(s) 293, 666, 778
BtsIMutI CAGTG 6 cut(s) 145, 293, 351, 666, 778, 1113
Cac8I GCNNGC 2 cut(s) 1044, 1189
CfoI GCGC 1 cut(s) 416
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 3 cut(s) 322, 323, 396
ClaI ATCGAT 1 cut(s) 1221
Csp6I GTAC 4 cut(s) 112, 484, 893, 1146
CspAI ACCGGT 1 cut(s) 383
CviAII CATG 1 cut(s) 29
CviQI GTAC 4 cut(s) 112, 484, 893, 1146
DdeI CTNAG 4 cut(s) 261, 990, 1026, 1236
DpnI GATC 6 cut(s) 350, 497, 678, 848, 975, 1071
DpnII GATC 6 cut(s) 348, 495, 676, 846, 973, 1069
Eam1104I CTCTTC 3 cut(s) 19, 288, 659
EarI CTCTTC 3 cut(s) 19, 288, 659
EciI GGCGGA 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 164
Eco105I TACGTA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 766
Eco147I AGGCCT 1 cut(s) 579
Eco24I GRGCYC 2 cut(s) 166, 326
Eco31I GGTCTC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 396
Eco53kI GAGCTC 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 1236
Eco88I CYCGRG 1 cut(s) 1202
EcoICRI GAGCTC 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO109I RGGNCCY 2 cut(s) 322, 323
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 4 cut(s) 325, 419, 533, 958
EcoT14I CCWWGG 1 cut(s) 766
EcoT38I GRGCYC 2 cut(s) 166, 326
ErhI CCWWGG 1 cut(s) 766
FaeI CATG 1 cut(s) 32
FaqI GGGAC 1 cut(s) 409
FatI CATG 1 cut(s) 28
FbaI TGATCA 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 72, 943, 1208
FriOI GRGCYC 2 cut(s) 166, 326
Fsp4HI GCNGC 3 cut(s) 72, 943, 1208
FspBI CTAG 3 cut(s) 213, 767, 1065
GlaI GCGC 1 cut(s) 415
GluI GCNGC 3 cut(s) 72, 943, 1208
GsaI CCCAGC 1 cut(s) 676
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 3 cut(s) 280, 324, 579
HapII CCGG 5 cut(s) 130, 180, 195, 384, 642
HhaI GCGC 1 cut(s) 416
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HinfI GANTC 8 cut(s) 5, 269, 632, 687, 789, 833, 994, 1177
HpaII CCGG 5 cut(s) 130, 180, 195, 384, 642
HphI GGTGA 2 cut(s) 448, 735
Hpy166II GTNNAC 1 cut(s) 953
Hpy188I TCNGA 3 cut(s) 161, 274, 348
Hpy188III TCNNGA 4 cut(s) 263, 436, 724, 989
Hpy8I GTNNAC 1 cut(s) 953
Hpy99I CGWCG 2 cut(s) 374, 377
HpyAV CCTTC 3 cut(s) 309, 327, 433
HpyCH4III ACNGT 3 cut(s) 140, 760, 1145
HpyCH4IV ACGT 3 cut(s) 110, 372, 924
HpyCH4V TGCA 4 cut(s) 593, 654, 659, 1089
HpyF10VI GCNNNNNNNGC 4 cut(s) 709, 761, 1167, 1207
HpyF3I CTNAG 4 cut(s) 261, 990, 1026, 1236
HpySE526I ACGT 3 cut(s) 110, 372, 924
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 1 cut(s) 414
Ksp22I TGATCA 1 cut(s) 348
Kzo9I GATC 6 cut(s) 348, 495, 676, 846, 973, 1069
LmnI GCTCC 3 cut(s) 161, 198, 421
Lsp1109I GCAGC 1 cut(s) 1219
LweI GCATC 3 cut(s) 232, 721, 1098
MaeI CTAG 3 cut(s) 213, 767, 1065
MaeII ACGT 3 cut(s) 110, 372, 924
MaeIII GTNAC 8 cut(s) 106, 182, 386, 454, 560, 814, 1033, 1174
MalI GATC 6 cut(s) 350, 497, 678, 848, 975, 1071
MboI GATC 6 cut(s) 348, 495, 676, 846, 973, 1069
MboII GAAGA 6 cut(s) 6, 275, 278, 676, 821, 922
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 4 cut(s) 145, 166, 177, 326
MluCI AATT 8 cut(s) 168, 378, 431, 523, 692, 1059, 1083, 1130
MlyI GAGTC 2 cut(s) 278, 1171
MmeI TCCRAC 1 cut(s) 1032
MseI TTAA 6 cut(s) 96, 123, 338, 624, 1263, 1285
MslI CAYNNNNRTG 2 cut(s) 240, 659
MspA1I CMGCKG 1 cut(s) 857
MspI CCGG 5 cut(s) 130, 180, 195, 384, 642
MspR9I CCNGG 7 cut(s) 180, 196, 327, 421, 535, 643, 960
Mva1269I GAATGC 1 cut(s) 705
MvaI CCWGG 4 cut(s) 327, 421, 535, 960
MwoI GCNNNNNNNGC 4 cut(s) 709, 761, 1167, 1207
NciI CCSGG 3 cut(s) 180, 196, 643
NdeII GATC 6 cut(s) 348, 495, 676, 846, 973, 1069
NlaIII CATG 1 cut(s) 32
NlaIV GGNNCC 2 cut(s) 324, 397
NmuCI GTSAC 3 cut(s) 454, 814, 1174
PceI AGGCCT 1 cut(s) 579
PctI GAATGC 1 cut(s) 705
PfeI GAWTC 5 cut(s) 632, 687, 789, 833, 994
PinAI ACCGGT 1 cut(s) 383
PkrI GCNGC 3 cut(s) 73, 944, 1209
PleI GAGTC 2 cut(s) 277, 1171
PpsI GAGTC 2 cut(s) 277, 1171
Ppu21I YACGTR 1 cut(s) 111
Psp124BI GAGCTC 1 cut(s) 166
Psp6I CCWGG 4 cut(s) 325, 419, 533, 958
PspFI CCCAGC 1 cut(s) 672
PspGI CCWGG 4 cut(s) 325, 419, 533, 958
PspN4I GGNNCC 2 cut(s) 324, 397
PspOMI GGGCCC 1 cut(s) 322
PspPI GGNCC 3 cut(s) 322, 323, 396
PsrI GAACNNNNNNTAC 2 cut(s) 349, 381
PsuI RGATCY 1 cut(s) 495
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 4 cut(s) 113, 485, 894, 1147
RsaNI GTAC 4 cut(s) 112, 484, 893, 1146
RseI CAYNNNNRTG 2 cut(s) 240, 659
SacI GAGCTC 1 cut(s) 166
SaqAI TTAA 6 cut(s) 96, 123, 338, 624, 1263, 1285
SatI GCNGC 3 cut(s) 72, 943, 1208
Sau3AI GATC 6 cut(s) 348, 495, 676, 846, 973, 1069
Sau96I GGNCC 3 cut(s) 322, 323, 396
SchI GAGTC 2 cut(s) 278, 1171
ScrFI CCNGG 7 cut(s) 180, 196, 327, 421, 535, 643, 960
SduI GDGCHC 4 cut(s) 145, 166, 177, 326
SfaNI GCATC 3 cut(s) 232, 721, 1098
SfcI CTRYAG 2 cut(s) 858, 1009
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 2 cut(s) 240, 659
SnaBI TACGTA 1 cut(s) 111
Sse9I AATT 8 cut(s) 168, 378, 431, 523, 692, 1059, 1083, 1130
SseBI AGGCCT 1 cut(s) 579
SsiI CCGC 3 cut(s) 71, 117, 942
SspI AATATT 1 cut(s) 94
SspMI CTAG 3 cut(s) 213, 767, 1065
SstI GAGCTC 1 cut(s) 166
StuI AGGCCT 1 cut(s) 579
StyD4I CCNGG 7 cut(s) 178, 194, 325, 419, 533, 641, 958
StyI CCWWGG 1 cut(s) 766
TaaI ACNGT 3 cut(s) 140, 760, 1145
TaiI ACGT 3 cut(s) 113, 375, 927
TaqI TCGA 1 cut(s) 1221
TasI AATT 8 cut(s) 168, 378, 431, 523, 692, 1059, 1083, 1130
TatI WGTACW 2 cut(s) 483, 1145
TauI GCSGC 2 cut(s) 74, 945
TfiI GAWTC 5 cut(s) 632, 687, 789, 833, 994
Tru1I TTAA 6 cut(s) 96, 123, 338, 624, 1263, 1285
Tru9I TTAA 6 cut(s) 96, 123, 338, 624, 1263, 1285
TscAI CASTG 6 cut(s) 145, 300, 358, 666, 778, 1120
TseFI GTSAC 3 cut(s) 454, 814, 1174
TseI GCWGC 1 cut(s) 1207
Tsp45I GTSAC 3 cut(s) 454, 814, 1174
TspDTI ATGAA 2 cut(s) 291, 932
TspGWI ACGGA 1 cut(s) 910
TspRI CASTG 6 cut(s) 145, 300, 358, 666, 778, 1120
VpaK11BI GGWCC 1 cut(s) 396
XagI CCTNNNNNAGG 1 cut(s) 236
XapI RAATTY 4 cut(s) 431, 523, 692, 1083
XmaJI CCTAGG 1 cut(s) 766
XspI CTAG 3 cut(s) 213, 767, 1065
ZraI GACGTC 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.