RLG00000008108

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002711
Physical Location & Seq
Reverse (-)
25673111 .. 25675295
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008108

Sequence Viewer

Length: 1407 bp
ATGGACTCAAAATCTTTAACAATGTCTCATGTTGCTACTATTGTTATACTGGGATTACTATTCCTGCAAGCGGCAGAATGTAGAATAAAGAATATTAAGGATGCAGTTACGTACTCCGCCATTAACTGCCGGAAGCACAGTGCTCTTTTGACTGATTTCGGAGCCGTTGGCGATGGAAAGACATTGAACACAAAGGCCTTTAAAGATGCAGTTGATAAACTAAGCCAGTATGCATCTGATGGCGGAGCACAGCTAATTGTGCCCTCCGGTAACTGGCTAACCGGAAGCTTCAACCTTACAAGCCACTTTACCCTTTTCCTCCATAAGGATGCTGTTATTCTTGGGTCTCAGGATGAGTCCGAGTGGCCTCTTCTTCCAGCACTGCCTTCATACGGGCAAGGGAGAGAGGCCCCTGGTGGAAGGTTAAGCAGTCTGATCTTTGGAACAAACCTTACGGATGTCGTAATTACCGGTAACAATGGCACCATTGATGGGCAAGGTGCTCCCTGGTGGGACAAATTCAAGAAAGGTCTTTTGAATGTCACCCGACCATACTTGGTTGATATTATGTACTCTAATCAGATCCAAATCTCCAATCTCACTTTTCGTAATTCTCCATCCTGGCACATCCATCCCATTTACAGCAGTAACATTACTATACAGGGCCTCACAATCCTTGCACCCGTTTCCGTTCCTAACACAGATGGGATTGACCCCGACTCGTGTTCACAAACTCGAATTGAAGACTGTTACATAGTCTCCGGGGATGACTGCATTGCAGTGAAGAGTGGCTGGGATCAGCATGGAATCAAATTTCGAATGCCAACCAAGCATCTTGTTATTAGGAGGCTTACCTGTATTTCACCTAAAAGTGCTACCGTTGCCCTAGGCAGTGAAATGTCTGGTGGAATCAGGGATGTAAGAGTTGAAGATGTGACAGCCATCAACACCGAATCCAGCATTAGGGTCAAAACATCTGTAGGCAGAGGAGCTTATGTAAAAGACATTTATGTACGAAGAATGACGTTGAACACTATGAAGTACGTTTTCTGGATGACAGGCGCCTACGGTTCACATCCAGGCGAGGGTTTTGATCCAAAGGCACTTCCTGTGATTCAAAACATCAACTATAGAGAGGTTGTTGCTGAGAATGTTACAATGCCTGCCCAGTTGGATGGAATTGCTAATGATCCTTTCAAGGGAATTTGCATCTCTAATGTGAGTATCTCACTCACTGAAAAGCCAAAGAAATTGTTGTGGAACTGTACTGATGTTGAGGGCGTTACGAGCAATGTGACTCCAAAGGCTTGCGATTTGTTGACCGAGAAGCAAAAACCAATCGACTGTGCTTTCCCTGAGGATAAGTTAGCCATTGAGGGCGTTAAGCTGACCACTTGTTTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

469

Amino Acids

51.04

Weight (kDa)

7.08

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 144 - 426 4.6e-36 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 482, 1061
AciI CCGC 3 cut(s) 71, 117, 243
AclWI GGATC 4 cut(s) 577, 804, 1088, 1184
AcsI RAATTY 3 cut(s) 518, 812, 1203
AcyI GRCGYC 1 cut(s) 1062
AfaI GTAC 5 cut(s) 113, 572, 1014, 1043, 1267
AfiI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 9 cut(s) 187, 292, 523, 538, 743, 929, 1030, 1118, 1198
AjnI CCWGG 4 cut(s) 412, 506, 620, 1078
AluBI AGCT 4 cut(s) 253, 288, 992, 1387
AluI AGCT 4 cut(s) 253, 288, 992, 1387
Alw21I GWGCWC 3 cut(s) 145, 250, 505
Alw26I GTCTC 3 cut(s) 30, 351, 763
AlwI GGATC 4 cut(s) 577, 804, 1088, 1184
AoxI GGCC 4 cut(s) 195, 365, 408, 664
ApoI RAATTY 3 cut(s) 518, 812, 1203
AsiGI ACCGGT 1 cut(s) 470
AspA2I CCTAGG 1 cut(s) 886
AspLEI GCGC 1 cut(s) 1064
AspS9I GGNCC 2 cut(s) 409, 664
AsuC2I CCSGG 1 cut(s) 763
AsuHPI GGTGA 2 cut(s) 535, 855
AsuII TTCGAA 1 cut(s) 817
AvrII CCTAGG 1 cut(s) 886
AxyI CCTNAGG 1 cut(s) 1356
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 482, 1061
BauI CACGAG 1 cut(s) 721
BbsI GAAGAC 1 cut(s) 750
Bbv12I GWGCWC 3 cut(s) 145, 250, 505
BccI CCATC 8 cut(s) 167, 233, 485, 625, 639, 698, 950, 1169
BceAI ACGGC 1 cut(s) 149
BciT130I CCWGG 4 cut(s) 414, 508, 622, 1080
BcnI CCSGG 1 cut(s) 763
BcoDI GTCTC 3 cut(s) 30, 351, 763
BfaI CTAG 1 cut(s) 887
BfmI CTRYAG 2 cut(s) 978, 1129
BfoI RGCGCY 1 cut(s) 1065
BisI GCNGC 1 cut(s) 72
BlnI CCTAGG 1 cut(s) 886
BlsI GCNGC 1 cut(s) 73
Bme1390I CCNGG 5 cut(s) 414, 508, 622, 763, 1080
BmgT120I GGNCC 2 cut(s) 409, 664
BmiI GGNNCC 4 cut(s) 163, 411, 484, 1063
BmrFI CCNGG 5 cut(s) 414, 508, 622, 763, 1080
BmrI ACTGGG 2 cut(s) 59, 1162
BmsI GCATC 6 cut(s) 91, 196, 242, 319, 841, 1218
BmuI ACTGGG 2 cut(s) 59, 1162
BpiI GAAGAC 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 817
BpuMI CCSGG 1 cut(s) 763
BsaAI YACGTR 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 1062
BsaI GGTCTC 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 412, 506, 762, 886
BsaWI WCCGGW 3 cut(s) 266, 281, 470
BsaXI ACNNNNNCTCC 4 cut(s) 743, 773, 981, 1011
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 4 cut(s) 54, 226, 278, 1168
Bse21I CCTNAGG 1 cut(s) 1356
Bse3DI GCAATG 2 cut(s) 774, 1297
Bse8I GATNNNNATC 1 cut(s) 587
BseBI CCWGG 4 cut(s) 414, 508, 622, 1080
BseDI CCNNGG 4 cut(s) 412, 506, 762, 886
BseJI GATNNNNATC 1 cut(s) 587
BseLI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
BseMI GCAATG 2 cut(s) 774, 1297
BseMII CTCAG 3 cut(s) 362, 1137, 1347
BseNI ACTGG 4 cut(s) 54, 226, 278, 1168
BseRI GAGGAG 1 cut(s) 1002
BseSI GKGCMC 1 cut(s) 264
BseYI CCCAGC 1 cut(s) 792
BshFI GGCC 4 cut(s) 197, 367, 410, 666
BshNI GGYRCC 2 cut(s) 482, 1061
BshTI ACCGGT 1 cut(s) 470
BsiHKAI GWGCWC 3 cut(s) 145, 250, 505
BsiSI CCGG 5 cut(s) 130, 267, 282, 471, 762
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
BsmAI GTCTC 3 cut(s) 30, 351, 763
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 4 cut(s) 197, 367, 410, 666
Bso31I GGTCTC 1 cut(s) 351
Bsp119I TTCGAA 1 cut(s) 817
Bsp1286I GDGCHC 4 cut(s) 145, 250, 264, 505
Bsp143I GATC 5 cut(s) 435, 582, 796, 1093, 1189
BspACI CCGC 3 cut(s) 71, 117, 243
BspANI GGCC 4 cut(s) 197, 367, 410, 666
BspCNI CTCAG 3 cut(s) 361, 1138, 1348
BspLI GGNNCC 4 cut(s) 163, 411, 484, 1063
BspPI GGATC 4 cut(s) 577, 804, 1088, 1184
BspT104I TTCGAA 1 cut(s) 817
BspT107I GGYRCC 2 cut(s) 482, 1061
BspTNI GGTCTC 1 cut(s) 351
BsrDI GCAATG 2 cut(s) 774, 1297
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 4 cut(s) 54, 226, 278, 1168
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 4 cut(s) 412, 506, 762, 886
BssMI GATC 5 cut(s) 435, 582, 796, 1093, 1189
BssNI GRCGYC 1 cut(s) 1062
BssSI CACGAG 1 cut(s) 721
BssT1I CCWWGG 1 cut(s) 886
Bst2BI CACGAG 1 cut(s) 721
Bst2UI CCWGG 4 cut(s) 414, 508, 622, 1080
Bst4CI ACNGT 6 cut(s) 140, 749, 880, 1070, 1265, 1346
Bst6I CTCTTC 2 cut(s) 375, 779
BstACI GRCGYC 1 cut(s) 1062
BstBAI YACGTR 1 cut(s) 111
BstBI TTCGAA 1 cut(s) 817
BstC8I GCNNGC 3 cut(s) 69, 1164, 1309
BstDEI CTNAG 4 cut(s) 221, 348, 1146, 1356
BstENI CCTNNNNNAGG 1 cut(s) 323
BstH2I RGCGCY 1 cut(s) 1065
BstHHI GCGC 1 cut(s) 1064
BstKTI GATC 5 cut(s) 438, 585, 799, 1096, 1192
BstMAI GTCTC 3 cut(s) 30, 351, 763
BstMBI GATC 5 cut(s) 435, 582, 796, 1093, 1189
BstMWI GCNNNNNNNGC 4 cut(s) 259, 829, 881, 1287
BstNI CCWGG 4 cut(s) 414, 508, 622, 1080
BstSCI CCNGG 5 cut(s) 412, 506, 620, 761, 1078
BstSFI CTRYAG 2 cut(s) 978, 1129
BstSLI GKGCMC 1 cut(s) 264
BstSNI TACGTA 1 cut(s) 111
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 1 cut(s) 582
BstXI CCANNNNNNTGG 1 cut(s) 1175
BstYI RGATCY 1 cut(s) 582
Bsu36I CCTNAGG 1 cut(s) 1356
BsuRI GGCC 4 cut(s) 197, 367, 410, 666
BtgZI GCGATG 1 cut(s) 186
BtsI GCAGTG 3 cut(s) 380, 786, 898
BtsIMutI CAGTG 5 cut(s) 145, 380, 786, 898, 1233
Cac8I GCNNGC 3 cut(s) 69, 1164, 1309
CfoI GCGC 1 cut(s) 1064
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 409, 664
Csp6I GTAC 5 cut(s) 112, 571, 1013, 1042, 1266
CspAI ACCGGT 1 cut(s) 470
CspCI CAANNNNNGTGG 1 cut(s) 1381
CviAII CATG 2 cut(s) 29, 803
CviQI GTAC 5 cut(s) 112, 571, 1013, 1042, 1266
DdeI CTNAG 4 cut(s) 221, 348, 1146, 1356
DinI GGCGCC 1 cut(s) 1063
DpnI GATC 5 cut(s) 437, 584, 798, 1095, 1191
DpnII GATC 5 cut(s) 435, 582, 796, 1093, 1189
DraI TTTAAA 1 cut(s) 202
Eam1104I CTCTTC 2 cut(s) 375, 779
EarI CTCTTC 2 cut(s) 375, 779
EciI GGCGGA 2 cut(s) 106, 258
Eco105I TACGTA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 886
Eco147I AGGCCT 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 351
Eco81I CCTNAGG 1 cut(s) 1356
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO109I RGGNCCY 2 cut(s) 409, 664
EcoRII CCWGG 4 cut(s) 412, 506, 620, 1078
EcoT14I CCWWGG 1 cut(s) 886
EcoT22I ATGCAT 1 cut(s) 235
EgeI GGCGCC 1 cut(s) 1063
EheI GGCGCC 1 cut(s) 1063
ErhI CCWWGG 1 cut(s) 886
FaeI CATG 2 cut(s) 32, 806
FaqI GGGAC 1 cut(s) 527
FatI CATG 2 cut(s) 28, 802
Fnu4HI GCNGC 1 cut(s) 72
Fsp4HI GCNGC 1 cut(s) 72
FspBI CTAG 1 cut(s) 887
GlaI GCGC 1 cut(s) 1063
GluI GCNGC 1 cut(s) 72
GsaI CCCAGC 1 cut(s) 796
HaeII RGCGCY 1 cut(s) 1065
HaeIII GGCC 4 cut(s) 197, 367, 410, 666
HapII CCGG 5 cut(s) 130, 267, 282, 471, 762
HhaI GCGC 1 cut(s) 1064
Hin1I GRCGYC 1 cut(s) 1062
Hin1II CATG 2 cut(s) 32, 806
Hin6I GCGC 1 cut(s) 1062
HinP1I GCGC 1 cut(s) 1062
HincII GTYRAC 1 cut(s) 1320
HindII GTYRAC 1 cut(s) 1320
HindIII AAGCTT 1 cut(s) 286
HinfI GANTC 8 cut(s) 5, 356, 719, 807, 909, 953, 1114, 1297
HpaII CCGG 5 cut(s) 130, 267, 282, 471, 762
HphI GGTGA 2 cut(s) 535, 855
Hpy166II GTNNAC 3 cut(s) 728, 1073, 1320
Hpy188I TCNGA 5 cut(s) 161, 238, 361, 435, 582
Hpy188III TCNNGA 3 cut(s) 350, 523, 1051
Hpy8I GTNNAC 3 cut(s) 728, 1073, 1320
HpyAV CCTTC 2 cut(s) 396, 414
HpyCH4III ACNGT 6 cut(s) 140, 749, 880, 1070, 1265, 1346
HpyCH4IV ACGT 3 cut(s) 110, 1025, 1044
HpyCH4V TGCA 8 cut(s) 67, 104, 209, 233, 680, 774, 779, 1209
HpyF10VI GCNNNNNNNGC 4 cut(s) 259, 829, 881, 1287
HpyF3I CTNAG 4 cut(s) 221, 348, 1146, 1356
HpySE526I ACGT 3 cut(s) 110, 1025, 1044
Hsp92I GRCGYC 1 cut(s) 1062
Hsp92II CATG 2 cut(s) 32, 806
HspAI GCGC 1 cut(s) 1062
KasI GGCGCC 1 cut(s) 1061
Kzo9I GATC 5 cut(s) 435, 582, 796, 1093, 1189
LmnI GCTCC 4 cut(s) 161, 245, 508, 989
LweI GCATC 6 cut(s) 91, 196, 242, 319, 841, 1218
MaeI CTAG 1 cut(s) 887
MaeII ACGT 3 cut(s) 110, 1025, 1044
MalI GATC 5 cut(s) 437, 584, 798, 1095, 1191
MboI GATC 5 cut(s) 435, 582, 796, 1093, 1189
MboII GAAGA 6 cut(s) 362, 365, 755, 796, 941, 1029
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 4 cut(s) 145, 250, 264, 505
MluCI AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
Mly113I GGCGCC 1 cut(s) 1062
MlyI GAGTC 3 cut(s) 365, 713, 1291
MmeI TCCRAC 1 cut(s) 1152
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 7 cut(s) 17, 96, 123, 201, 425, 1383, 1405
MslI CAYNNNNRTG 2 cut(s) 327, 779
MspI CCGG 5 cut(s) 130, 267, 282, 471, 762
MspR9I CCNGG 5 cut(s) 414, 508, 622, 763, 1080
Mva1269I GAATGC 1 cut(s) 825
MvaI CCWGG 4 cut(s) 414, 508, 622, 1080
MwoI GCNNNNNNNGC 4 cut(s) 259, 829, 881, 1287
NarI GGCGCC 1 cut(s) 1062
NciI CCSGG 1 cut(s) 763
NdeII GATC 5 cut(s) 435, 582, 796, 1093, 1189
NlaIII CATG 2 cut(s) 32, 806
NlaIV GGNNCC 4 cut(s) 163, 411, 484, 1063
NmuCI GTSAC 3 cut(s) 541, 934, 1294
NsiI ATGCAT 1 cut(s) 235
NspV TTCGAA 1 cut(s) 817
PceI AGGCCT 1 cut(s) 197
PctI GAATGC 1 cut(s) 825
PfeI GAWTC 4 cut(s) 807, 909, 953, 1114
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 1 cut(s) 73
PleI GAGTC 3 cut(s) 364, 713, 1291
PluTI GGCGCC 1 cut(s) 1065
PpsI GAGTC 3 cut(s) 364, 713, 1291
Ppu21I YACGTR 1 cut(s) 111
Psp6I CCWGG 4 cut(s) 412, 506, 620, 1078
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 4 cut(s) 412, 506, 620, 1078
PspN4I GGNNCC 4 cut(s) 163, 411, 484, 1063
PspPI GGNCC 2 cut(s) 409, 664
PsrI GAACNNNNNNTAC 2 cut(s) 436, 468
PsuI RGATCY 1 cut(s) 582
RsaI GTAC 5 cut(s) 113, 572, 1014, 1043, 1267
RsaNI GTAC 5 cut(s) 112, 571, 1013, 1042, 1266
RseI CAYNNNNRTG 2 cut(s) 327, 779
SaqAI TTAA 7 cut(s) 17, 96, 123, 201, 425, 1383, 1405
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 5 cut(s) 435, 582, 796, 1093, 1189
Sau96I GGNCC 2 cut(s) 409, 664
SchI GAGTC 3 cut(s) 365, 713, 1291
ScrFI CCNGG 5 cut(s) 414, 508, 622, 763, 1080
SduI GDGCHC 4 cut(s) 145, 250, 264, 505
SfaNI GCATC 6 cut(s) 91, 196, 242, 319, 841, 1218
SfcI CTRYAG 2 cut(s) 978, 1129
SfoI GGCGCC 1 cut(s) 1063
SfuI TTCGAA 1 cut(s) 817
SmiMI CAYNNNNRTG 2 cut(s) 327, 779
SnaBI TACGTA 1 cut(s) 111
Sse9I AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
SseBI AGGCCT 1 cut(s) 197
SsiI CCGC 3 cut(s) 71, 117, 243
SspDI GGCGCC 1 cut(s) 1061
SspI AATATT 1 cut(s) 94
SspMI CTAG 1 cut(s) 887
StuI AGGCCT 1 cut(s) 197
StyD4I CCNGG 5 cut(s) 412, 506, 620, 761, 1078
StyI CCWWGG 1 cut(s) 886
TaaI ACNGT 6 cut(s) 140, 749, 880, 1070, 1265, 1346
TaiI ACGT 3 cut(s) 113, 1028, 1047
TaqI TCGA 3 cut(s) 736, 817, 1341
TaqII GACCGA 1 cut(s) 1337
TasI AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
TatI WGTACW 2 cut(s) 570, 1265
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 4 cut(s) 807, 909, 953, 1114
Tru1I TTAA 7 cut(s) 17, 96, 123, 201, 425, 1383, 1405
Tru9I TTAA 7 cut(s) 17, 96, 123, 201, 425, 1383, 1405
TscAI CASTG 5 cut(s) 145, 387, 786, 898, 1240
TseFI GTSAC 3 cut(s) 541, 934, 1294
Tsp45I GTSAC 3 cut(s) 541, 934, 1294
TspDTI ATGAA 2 cut(s) 378, 1052
TspGWI ACGGA 2 cut(s) 470, 679
TspRI CASTG 5 cut(s) 145, 387, 786, 898, 1240
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 3 cut(s) 518, 812, 1203
XmaJI CCTAGG 1 cut(s) 886
XspI CTAG 1 cut(s) 887
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.