Rw4G016220

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002711
Physical Location & Seq
Forward (+)
37226842 .. 37229979
3138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016220.1

Sequence Viewer

Length: 1413 bp
ATGATTTTTCAAAAACCGATTTTGAAAATAAAAACGAGAAAACAAGATTGGATTTTGAGATCCGAAAATGTTGCTACTATTGTTATACTGGGATTACTATTCCTGCAAGCGGCAGAATGTAGAGTAGAGAATATTAAGGATGCAGTTACGTACTCCGCCATTAACTGCCGGAAGCACAGTGCTCTTTTGACTGATTTCGGAGCCGTTGGCGATGGAAAGACATTGAACACAAAGGCTTTTAAAGATGCAGTTGATAAACTAAGCCAGTATGCATCTGATGGCGGAGCACAGCTAATTGTGCCCCCCGGTAACTGGCTTACCGGAAGCTTCAACCTCACAAGCCACTTTACCCTTTTCCTCCATAAGGATGCTGTTATTCTTGGGTCTCAGGATGAGTCCGAGTGGCCTCTTCTTCCAGCACTGCCTTCATACGGGCAAGGGAGAGAGGCCCCTGGTGGAAGGTTAAGCAGTCTAATCTTTGGAACAAACCTTACCGATGTCGTAATTACAGGTAACAATGGCACCATTGATGGGCAAGGTGCTCCCTGGTGGGACAAATTCAAGAAGGGTCTTTTGAAAGTCACCCGACCATACTTGGTTGATATTATGTACTCTAATCAGATCCAAATCTCCAATCTCACTTTTCGTAATTCTCCATCCTGGCACATCCATCCCATTTACAGCAGTAACATTACTATACAGGGCCTCACAATCCTTGCACCCGTTACCGTTCCTAACACAGATGGTATTGACCCCGACTCGTTCTCCGGGGATGACTGCATTGCAGTGAAGAGTGGCTGGGATCAGCATGGAATCAAATTTCGAATGCCAACCAAGCATCTTGTTATTAGGAGGCTTACCTGTATTTCACCTAAAAGTGCTACCGTTGCCCTAGGCAGTGAAATGTCTGGTGGAATCAGGGATGTAAGAGTTGAAGATGTGACAGCCATTAACACCGAATCCAGCATTAGGATCAAAACATCTGTAGGCAGAGGAGCTTATGTAAAAGACATTTATGTACGAAGAATGACGTTGAACACTATGAAGTTCGTTTTTTGGATGACAGGCGCCTATGGTTCACATCCAGGAGAGGGTTTTGATCCAAAGGCACTTCCTGTGATTCAAAACATCAACTATAGAGAGGTTGTTGCTGAGAATGTTACAATGCCGGCCCAGTTGGATGGAATTGCTAGTGATCCTTTCAAGGGAATTTGCATCTCTAATGTGAGTATCTCACTCACTGAAAAGCCAAAGAAATTGTTGTGGAACTGTACTGATGTTGAGGGCGTTACGAGCAATGTGACTCCAAAGGCTTGCGATTCGTTGCCCGAGCAGCAAAAACCAATCGATTGTGCTTTCCCTGAGGATAAGTTAGCCATTGAGGGCGTTAAGCTGACCACTTGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

470

Amino Acids

51.43

Weight (kDa)

8.25

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 158 - 428 2.5e-26 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 521, 1067
AciI CCGC 3 cut(s) 110, 156, 282
AclWI GGATC 6 cut(s) 54, 616, 810, 980, 1094, 1190
AcsI RAATTY 3 cut(s) 557, 818, 1209
AcyI GRCGYC 1 cut(s) 1068
AfaI GTAC 4 cut(s) 152, 611, 1020, 1273
AfiI CCNNNNNNNGG 8 cut(s) 109, 312, 364, 431, 531, 969, 1091, 1205
AjnI CCWGG 4 cut(s) 451, 545, 659, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 4 cut(s) 292, 327, 998, 1393
AluI AGCT 4 cut(s) 292, 327, 998, 1393
Alw21I GWGCWC 3 cut(s) 184, 289, 544
Alw26I GTCTC 1 cut(s) 390
AlwI GGATC 6 cut(s) 54, 616, 810, 980, 1094, 1190
Ama87I CYCGRG 1 cut(s) 1328
AoxI GGCC 4 cut(s) 404, 447, 703, 1170
ApeKI GCWGC 1 cut(s) 1333
ApoI RAATTY 3 cut(s) 557, 818, 1209
AspA2I CCTAGG 1 cut(s) 892
AspLEI GCGC 1 cut(s) 1070
AspS9I GGNCC 3 cut(s) 448, 703, 1171
AsuC2I CCSGG 2 cut(s) 306, 769
AsuHPI GGTGA 2 cut(s) 574, 861
AsuII TTCGAA 1 cut(s) 823
AvaI CYCGRG 1 cut(s) 1328
AvrII CCTAGG 1 cut(s) 892
AxyI CCTNAGG 1 cut(s) 1362
BaeGI GKGCMC 1 cut(s) 303
BanI GGYRCC 2 cut(s) 521, 1067
Bbv12I GWGCWC 3 cut(s) 184, 289, 544
BbvI GCAGC 1 cut(s) 1345
BccI CCATC 7 cut(s) 206, 272, 524, 664, 678, 737, 1175
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 4 cut(s) 453, 547, 661, 1086
BcnI CCSGG 2 cut(s) 306, 769
BcoDI GTCTC 1 cut(s) 390
BfaI CTAG 2 cut(s) 893, 1191
BfmI CTRYAG 2 cut(s) 984, 1135
BfoI RGCGCY 1 cut(s) 1071
BisI GCNGC 2 cut(s) 111, 1334
BlnI CCTAGG 1 cut(s) 892
BlsI GCNGC 2 cut(s) 112, 1335
Bme1390I CCNGG 6 cut(s) 306, 453, 547, 661, 769, 1086
BmeT110I CYCGRG 1 cut(s) 1328
BmgT120I GGNCC 3 cut(s) 448, 703, 1171
BmiI GGNNCC 4 cut(s) 202, 450, 523, 1069
BmrFI CCNGG 6 cut(s) 306, 453, 547, 661, 769, 1086
BmrI ACTGGG 2 cut(s) 98, 1168
BmsI GCATC 6 cut(s) 130, 235, 281, 358, 847, 1224
BmuI ACTGGG 2 cut(s) 98, 1168
Bpu14I TTCGAA 1 cut(s) 823
BpuMI CCSGG 2 cut(s) 306, 769
Bsa29I ATCGAT 1 cut(s) 1347
BsaAI YACGTR 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 626
BsaHI GRCGYC 1 cut(s) 1068
BsaI GGTCTC 1 cut(s) 390
BsaJI CCNNGG 5 cut(s) 304, 451, 545, 768, 892
BsaWI WCCGGW 1 cut(s) 320
BsaXI ACNNNNNCTCC 4 cut(s) 749, 779, 987, 1017
Bsc4I CCNNNNNNNGG 8 cut(s) 109, 312, 364, 431, 531, 969, 1091, 1205
Bse118I RCCGGY 1 cut(s) 1168
Bse1I ACTGG 4 cut(s) 93, 265, 317, 1174
Bse21I CCTNAGG 1 cut(s) 1362
Bse3DI GCAATG 2 cut(s) 780, 1303
Bse8I GATNNNNATC 1 cut(s) 626
BseBI CCWGG 4 cut(s) 453, 547, 661, 1086
BseCI ATCGAT 1 cut(s) 1347
BseDI CCNNGG 5 cut(s) 304, 451, 545, 768, 892
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 8 cut(s) 109, 312, 364, 431, 531, 969, 1091, 1205
BseMI GCAATG 2 cut(s) 780, 1303
BseMII CTCAG 3 cut(s) 401, 1143, 1353
BseNI ACTGG 4 cut(s) 93, 265, 317, 1174
BseRI GAGGAG 1 cut(s) 1008
BseSI GKGCMC 1 cut(s) 303
BseXI GCAGC 1 cut(s) 1345
BseYI CCCAGC 1 cut(s) 798
BshFI GGCC 4 cut(s) 406, 449, 705, 1172
BshNI GGYRCC 2 cut(s) 521, 1067
BshVI ATCGAT 1 cut(s) 1347
BsiHKAI GWGCWC 3 cut(s) 184, 289, 544
BsiHKCI CYCGRG 1 cut(s) 1328
BsiSI CCGG 5 cut(s) 169, 306, 321, 768, 1169
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 8 cut(s) 109, 312, 364, 431, 531, 969, 1091, 1205
BsmAI GTCTC 1 cut(s) 390
BsmFI GGGAC 1 cut(s) 566
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 4 cut(s) 406, 449, 705, 1172
Bso31I GGTCTC 1 cut(s) 390
BsoBI CYCGRG 1 cut(s) 1328
Bsp119I TTCGAA 1 cut(s) 823
Bsp1286I GDGCHC 4 cut(s) 184, 289, 303, 544
Bsp143I GATC 6 cut(s) 59, 621, 802, 972, 1099, 1195
BspACI CCGC 3 cut(s) 110, 156, 282
BspANI GGCC 4 cut(s) 406, 449, 705, 1172
BspCNI CTCAG 3 cut(s) 400, 1144, 1354
BspDI ATCGAT 1 cut(s) 1347
BspLI GGNNCC 4 cut(s) 202, 450, 523, 1069
BspPI GGATC 6 cut(s) 54, 616, 810, 980, 1094, 1190
BspT104I TTCGAA 1 cut(s) 823
BspT107I GGYRCC 2 cut(s) 521, 1067
BspTNI GGTCTC 1 cut(s) 390
BsrDI GCAATG 2 cut(s) 780, 1303
BsrFI RCCGGY 1 cut(s) 1168
BsrI ACTGG 4 cut(s) 93, 265, 317, 1174
BssAI RCCGGY 1 cut(s) 1168
BssECI CCNNGG 5 cut(s) 304, 451, 545, 768, 892
BssMI GATC 6 cut(s) 59, 621, 802, 972, 1099, 1195
BssNI GRCGYC 1 cut(s) 1068
BssT1I CCWWGG 1 cut(s) 892
Bst2UI CCWGG 4 cut(s) 453, 547, 661, 1086
Bst4CI ACNGT 4 cut(s) 179, 730, 886, 1271
Bst6I CTCTTC 2 cut(s) 414, 785
BstACI GRCGYC 1 cut(s) 1068
BstBAI YACGTR 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 823
BstC8I GCNNGC 3 cut(s) 108, 1170, 1315
BstDEI CTNAG 4 cut(s) 260, 387, 1152, 1362
BstENI CCTNNNNNAGG 1 cut(s) 362
BstH2I RGCGCY 1 cut(s) 1071
BstHHI GCGC 1 cut(s) 1070
BstKTI GATC 6 cut(s) 62, 624, 805, 975, 1102, 1198
BstMAI GTCTC 1 cut(s) 390
BstMBI GATC 6 cut(s) 59, 621, 802, 972, 1099, 1195
BstMWI GCNNNNNNNGC 5 cut(s) 298, 835, 887, 1293, 1333
BstNI CCWGG 4 cut(s) 453, 547, 661, 1086
BstSCI CCNGG 6 cut(s) 304, 451, 545, 659, 767, 1084
BstSFI CTRYAG 2 cut(s) 984, 1135
BstSLI GKGCMC 1 cut(s) 303
BstSNI TACGTA 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 1345
BstX2I RGATCY 2 cut(s) 59, 621
BstXI CCANNNNNNTGG 1 cut(s) 1181
BstYI RGATCY 2 cut(s) 59, 621
Bsu15I ATCGAT 1 cut(s) 1347
Bsu36I CCTNAGG 1 cut(s) 1362
BsuRI GGCC 4 cut(s) 406, 449, 705, 1172
BsuTUI ATCGAT 1 cut(s) 1347
BtgZI GCGATG 1 cut(s) 225
BtsI GCAGTG 3 cut(s) 419, 792, 904
BtsIMutI CAGTG 5 cut(s) 184, 419, 792, 904, 1239
Cac8I GCNNGC 3 cut(s) 108, 1170, 1315
CfoI GCGC 1 cut(s) 1070
Cfr10I RCCGGY 1 cut(s) 1168
Cfr13I GGNCC 3 cut(s) 448, 703, 1171
ClaI ATCGAT 1 cut(s) 1347
Csp6I GTAC 4 cut(s) 151, 610, 1019, 1272
CviAII CATG 1 cut(s) 809
CviQI GTAC 4 cut(s) 151, 610, 1019, 1272
DdeI CTNAG 4 cut(s) 260, 387, 1152, 1362
DinI GGCGCC 1 cut(s) 1069
DpnI GATC 6 cut(s) 61, 623, 804, 974, 1101, 1197
DpnII GATC 6 cut(s) 59, 621, 802, 972, 1099, 1195
DraI TTTAAA 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 414, 785
EarI CTCTTC 2 cut(s) 414, 785
EciI GGCGGA 2 cut(s) 145, 297
Eco105I TACGTA 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 892
Eco31I GGTCTC 1 cut(s) 390
Eco81I CCTNAGG 1 cut(s) 1362
Eco88I CYCGRG 1 cut(s) 1328
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoO109I RGGNCCY 2 cut(s) 448, 703
EcoRII CCWGG 4 cut(s) 451, 545, 659, 1084
EcoT14I CCWWGG 1 cut(s) 892
EcoT22I ATGCAT 1 cut(s) 274
EgeI GGCGCC 1 cut(s) 1069
EheI GGCGCC 1 cut(s) 1069
ErhI CCWWGG 1 cut(s) 892
FaeI CATG 1 cut(s) 812
FaqI GGGAC 1 cut(s) 566
FatI CATG 1 cut(s) 808
Fnu4HI GCNGC 2 cut(s) 111, 1334
Fsp4HI GCNGC 2 cut(s) 111, 1334
FspBI CTAG 2 cut(s) 893, 1191
GlaI GCGC 1 cut(s) 1069
GluI GCNGC 2 cut(s) 111, 1334
GsaI CCCAGC 1 cut(s) 802
HaeII RGCGCY 1 cut(s) 1071
HaeIII GGCC 4 cut(s) 406, 449, 705, 1172
HapII CCGG 5 cut(s) 169, 306, 321, 768, 1169
HhaI GCGC 1 cut(s) 1070
Hin1I GRCGYC 1 cut(s) 1068
Hin1II CATG 1 cut(s) 812
Hin6I GCGC 1 cut(s) 1068
HinP1I GCGC 1 cut(s) 1068
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 8 cut(s) 395, 758, 813, 915, 959, 1120, 1303, 1319
HpaII CCGG 5 cut(s) 169, 306, 321, 768, 1169
HphI GGTGA 2 cut(s) 574, 861
Hpy166II GTNNAC 1 cut(s) 1079
Hpy188I TCNGA 5 cut(s) 64, 200, 277, 400, 621
Hpy188III TCNNGA 2 cut(s) 389, 562
Hpy8I GTNNAC 1 cut(s) 1079
HpyAV CCTTC 3 cut(s) 435, 453, 559
HpyCH4III ACNGT 4 cut(s) 179, 730, 886, 1271
HpyCH4IV ACGT 2 cut(s) 149, 1031
HpyCH4V TGCA 8 cut(s) 106, 143, 248, 272, 719, 780, 785, 1215
HpyF10VI GCNNNNNNNGC 5 cut(s) 298, 835, 887, 1293, 1333
HpyF3I CTNAG 4 cut(s) 260, 387, 1152, 1362
HpySE526I ACGT 2 cut(s) 149, 1031
Hsp92I GRCGYC 1 cut(s) 1068
Hsp92II CATG 1 cut(s) 812
HspAI GCGC 1 cut(s) 1068
KasI GGCGCC 1 cut(s) 1067
KroI GCCGGC 1 cut(s) 1168
KroNI GCCGGC 1 cut(s) 1170
Kzo9I GATC 6 cut(s) 59, 621, 802, 972, 1099, 1195
LmnI GCTCC 4 cut(s) 200, 284, 547, 995
Lsp1109I GCAGC 1 cut(s) 1345
LweI GCATC 6 cut(s) 130, 235, 281, 358, 847, 1224
MaeI CTAG 2 cut(s) 893, 1191
MaeII ACGT 2 cut(s) 149, 1031
MalI GATC 6 cut(s) 61, 623, 804, 974, 1101, 1197
MboI GATC 6 cut(s) 59, 621, 802, 972, 1099, 1195
MboII GAAGA 5 cut(s) 401, 404, 802, 947, 1035
MflI RGATCY 2 cut(s) 59, 621
MhlI GDGCHC 4 cut(s) 184, 289, 303, 544
MluCI AATT 8 cut(s) 294, 504, 557, 649, 818, 1185, 1209, 1256
Mly113I GGCGCC 1 cut(s) 1068
MlyI GAGTC 3 cut(s) 404, 752, 1297
MmeI TCCRAC 1 cut(s) 1158
Mph1103I ATGCAT 1 cut(s) 274
MroNI GCCGGC 1 cut(s) 1168
MseI TTAA 7 cut(s) 135, 162, 240, 464, 951, 1389, 1411
MslI CAYNNNNRTG 2 cut(s) 366, 785
MspI CCGG 5 cut(s) 169, 306, 321, 768, 1169
MspR9I CCNGG 6 cut(s) 306, 453, 547, 661, 769, 1086
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 4 cut(s) 453, 547, 661, 1086
MwoI GCNNNNNNNGC 5 cut(s) 298, 835, 887, 1293, 1333
NaeI GCCGGC 1 cut(s) 1170
NarI GGCGCC 1 cut(s) 1068
NciI CCSGG 2 cut(s) 306, 769
NdeII GATC 6 cut(s) 59, 621, 802, 972, 1099, 1195
NgoMIV GCCGGC 1 cut(s) 1168
NlaIII CATG 1 cut(s) 812
NlaIV GGNNCC 4 cut(s) 202, 450, 523, 1069
NmuCI GTSAC 3 cut(s) 580, 940, 1300
NsiI ATGCAT 1 cut(s) 274
NspV TTCGAA 1 cut(s) 823
PctI GAATGC 1 cut(s) 831
PdiI GCCGGC 1 cut(s) 1170
PfeI GAWTC 5 cut(s) 813, 915, 959, 1120, 1319
PfoI TCCNGGA 1 cut(s) 1084
PkrI GCNGC 2 cut(s) 112, 1335
PleI GAGTC 3 cut(s) 403, 752, 1297
PluTI GGCGCC 1 cut(s) 1071
PpsI GAGTC 3 cut(s) 403, 752, 1297
Ppu21I YACGTR 1 cut(s) 150
Psp6I CCWGG 4 cut(s) 451, 545, 659, 1084
PspFI CCCAGC 1 cut(s) 798
PspGI CCWGG 4 cut(s) 451, 545, 659, 1084
PspN4I GGNNCC 4 cut(s) 202, 450, 523, 1069
PspPI GGNCC 3 cut(s) 448, 703, 1171
PsrI GAACNNNNNNTAC 2 cut(s) 475, 507
PsuI RGATCY 2 cut(s) 59, 621
RsaI GTAC 4 cut(s) 152, 611, 1020, 1273
RsaNI GTAC 4 cut(s) 151, 610, 1019, 1272
RseI CAYNNNNRTG 2 cut(s) 366, 785
SaqAI TTAA 7 cut(s) 135, 162, 240, 464, 951, 1389, 1411
SatI GCNGC 2 cut(s) 111, 1334
Sau3AI GATC 6 cut(s) 59, 621, 802, 972, 1099, 1195
Sau96I GGNCC 3 cut(s) 448, 703, 1171
SchI GAGTC 3 cut(s) 404, 752, 1297
ScrFI CCNGG 6 cut(s) 306, 453, 547, 661, 769, 1086
SduI GDGCHC 4 cut(s) 184, 289, 303, 544
SfaNI GCATC 6 cut(s) 130, 235, 281, 358, 847, 1224
SfcI CTRYAG 2 cut(s) 984, 1135
SfoI GGCGCC 1 cut(s) 1069
SfuI TTCGAA 1 cut(s) 823
SmiMI CAYNNNNRTG 2 cut(s) 366, 785
SnaBI TACGTA 1 cut(s) 150
Sse9I AATT 8 cut(s) 294, 504, 557, 649, 818, 1185, 1209, 1256
SsiI CCGC 3 cut(s) 110, 156, 282
SspDI GGCGCC 1 cut(s) 1067
SspI AATATT 1 cut(s) 133
SspMI CTAG 2 cut(s) 893, 1191
StyD4I CCNGG 6 cut(s) 304, 451, 545, 659, 767, 1084
StyI CCWWGG 1 cut(s) 892
TaaI ACNGT 4 cut(s) 179, 730, 886, 1271
TaiI ACGT 2 cut(s) 152, 1034
TaqI TCGA 2 cut(s) 823, 1347
TasI AATT 8 cut(s) 294, 504, 557, 649, 818, 1185, 1209, 1256
TatI WGTACW 2 cut(s) 609, 1271
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 5 cut(s) 813, 915, 959, 1120, 1319
Tru1I TTAA 7 cut(s) 135, 162, 240, 464, 951, 1389, 1411
Tru9I TTAA 7 cut(s) 135, 162, 240, 464, 951, 1389, 1411
TscAI CASTG 5 cut(s) 184, 426, 792, 904, 1246
TseFI GTSAC 3 cut(s) 580, 940, 1300
TseI GCWGC 1 cut(s) 1333
Tsp45I GTSAC 3 cut(s) 580, 940, 1300
TspDTI ATGAA 2 cut(s) 417, 1058
TspRI CASTG 5 cut(s) 184, 426, 792, 904, 1246
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 3 cut(s) 557, 818, 1209
XmaJI CCTAGG 1 cut(s) 892
XspI CTAG 2 cut(s) 893, 1191
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.