Rh4DG189100

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002711
Physical Location & Seq
Forward (+)
37328667 .. 37330725
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG189100.1

Sequence Viewer

Length: 1407 bp
ATGGACTCAAAATCTTTAACAATGTCTCATGTTGCTACTATTGTTATACTGGGATTACTATTCCTGCAAGCGGCAGAATGTAGAGTAGAGAATATTAAGGATGCAGTTACGTACTCCGCCATTAACTGCCGGAAGCACAGTGCTCTTTTGACTGATTTCGGAGCCGTTGGCGATGGAAAGACATTGAACACAAAGGCTTTTAAAGATGCAGTTGATAAACTAAGCCAGTATGCATCTGATGGCGGAGCACAGCTAATTGTGCCCCCCGGTAACTGGCTTACCGGAAGCTTCAACCTCACAAGCCACTTTACCCTTTTCCTCCATAAGGATGCTGTTATTCTTGGGTCTCAGGATGAGTCCGAGTGGCCTCTTCTTCCAGCACTGCCTTCATACGGGCAAGGGAGAGAGGCCCCTGGTGGAAGGTTAAGCAGTCTAATCTTTGGAACAAACCTTACCGATGTCGTAATTACAGGTAACAATGGCACCATTGATGGGCAAGGTGCTCCCTGGTGGGACAAATTCAAGAAGGGTCTTTTGAAAGTCACCCGACCATACTTGGTTGATATTATGTACTCTAATCAGATCCAAATCTCCAATCTCACTTTTCGTAATTCTCCATCCTGGCACATCCATCCCATTTACAGCAGTAACATTACTATACAGGGCCTCACAATCCTTGCACCCGTTACCGTTCCTAACACAGATGGTATTGACCCCGACTCGTGTTCACAAACTCGAATTGAAGACTGTTACATAGTCTCCGGGGATGACTGCATTGCAGTGAAGAGTGGCTGGGATCAGCATGGAATCAAATTTCGAATGCCAACCAAGCATCTTGTTATTAGGAGGCTTACCTGTATTTCACCTAAAAGTGCTACCGTTGCCCTAGGCAGTGAAATGTCTGGTGGAATCAGGGATGTAAGAGTTGAAGATGTGACAGCCATTAACACCGAATCCAGCATTAGGATCAAAACATCTGTAGGCAGAGGAGCTTATGTAAAAGACATTTATGTACGAAGAATGACGTTGAACACTATGAAGTTCGTTTTTTGGATGACAGGCGCCTATGGTTCACATCCAGGAGAGGGTTTTGATCCAAAGGCACTTCCTGTGATTCAAAACATCAACTATAGAGAGGTTGTTGCTGAGAATGTTACAATGCCGGCCCAGTTGGATGGAATTGCTAGTGATCCTTTCAAGGGAATTTGCATCTCTAATGTGAGTATCTCACTCACTGAAAAGCCAAAGAAATTGTTGTGGAACTGTACTGATGTTGAGGGCGTTACGAGCAATGTGACTCCAAAGGCTTGCGATTCGTTGCCCGAGCAGCAAAAACCAATCGATTGTGCTTTCCCTGAGGATAAGTTAGCCATTGAGGGCGTTAAGCTGACCACTTGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

468

Amino Acids

50.95

Weight (kDa)

6.51

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 145 - 426 2.1e-35 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 482, 1061
AciI CCGC 3 cut(s) 71, 117, 243
AclWI GGATC 5 cut(s) 577, 804, 974, 1088, 1184
AcsI RAATTY 3 cut(s) 518, 812, 1203
AcyI GRCGYC 1 cut(s) 1062
AfaI GTAC 4 cut(s) 113, 572, 1014, 1267
AfiI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
AgsI TTSAA 9 cut(s) 187, 292, 523, 538, 743, 929, 1030, 1118, 1198
AjnI CCWGG 4 cut(s) 412, 506, 620, 1078
AluBI AGCT 4 cut(s) 253, 288, 992, 1387
AluI AGCT 4 cut(s) 253, 288, 992, 1387
Alw21I GWGCWC 3 cut(s) 145, 250, 505
Alw26I GTCTC 3 cut(s) 30, 351, 763
AlwI GGATC 5 cut(s) 577, 804, 974, 1088, 1184
Ama87I CYCGRG 1 cut(s) 1322
AoxI GGCC 4 cut(s) 365, 408, 664, 1164
ApeKI GCWGC 1 cut(s) 1327
ApoI RAATTY 3 cut(s) 518, 812, 1203
AspA2I CCTAGG 1 cut(s) 886
AspLEI GCGC 1 cut(s) 1064
AspS9I GGNCC 3 cut(s) 409, 664, 1165
AsuC2I CCSGG 2 cut(s) 267, 763
AsuHPI GGTGA 2 cut(s) 535, 855
AsuII TTCGAA 1 cut(s) 817
AvaI CYCGRG 1 cut(s) 1322
AvrII CCTAGG 1 cut(s) 886
AxyI CCTNAGG 1 cut(s) 1356
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 482, 1061
BauI CACGAG 1 cut(s) 721
BbsI GAAGAC 1 cut(s) 750
Bbv12I GWGCWC 3 cut(s) 145, 250, 505
BbvI GCAGC 1 cut(s) 1339
BccI CCATC 7 cut(s) 167, 233, 485, 625, 639, 698, 1169
BceAI ACGGC 1 cut(s) 149
BciT130I CCWGG 4 cut(s) 414, 508, 622, 1080
BcnI CCSGG 2 cut(s) 267, 763
BcoDI GTCTC 3 cut(s) 30, 351, 763
BfaI CTAG 2 cut(s) 887, 1185
BfmI CTRYAG 2 cut(s) 978, 1129
BfoI RGCGCY 1 cut(s) 1065
BisI GCNGC 2 cut(s) 72, 1328
BlnI CCTAGG 1 cut(s) 886
BlsI GCNGC 2 cut(s) 73, 1329
Bme1390I CCNGG 6 cut(s) 267, 414, 508, 622, 763, 1080
BmeT110I CYCGRG 1 cut(s) 1322
BmgT120I GGNCC 3 cut(s) 409, 664, 1165
BmiI GGNNCC 4 cut(s) 163, 411, 484, 1063
BmrFI CCNGG 6 cut(s) 267, 414, 508, 622, 763, 1080
BmrI ACTGGG 2 cut(s) 59, 1162
BmsI GCATC 6 cut(s) 91, 196, 242, 319, 841, 1218
BmuI ACTGGG 2 cut(s) 59, 1162
BpiI GAAGAC 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 817
BpuMI CCSGG 2 cut(s) 267, 763
Bsa29I ATCGAT 1 cut(s) 1341
BsaAI YACGTR 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 1062
BsaI GGTCTC 1 cut(s) 351
BsaJI CCNNGG 5 cut(s) 265, 412, 506, 762, 886
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 4 cut(s) 743, 773, 981, 1011
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
Bse118I RCCGGY 1 cut(s) 1162
Bse1I ACTGG 4 cut(s) 54, 226, 278, 1168
Bse21I CCTNAGG 1 cut(s) 1356
Bse3DI GCAATG 2 cut(s) 774, 1297
Bse8I GATNNNNATC 1 cut(s) 587
BseBI CCWGG 4 cut(s) 414, 508, 622, 1080
BseCI ATCGAT 1 cut(s) 1341
BseDI CCNNGG 5 cut(s) 265, 412, 506, 762, 886
BseJI GATNNNNATC 1 cut(s) 587
BseLI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
BseMI GCAATG 2 cut(s) 774, 1297
BseMII CTCAG 3 cut(s) 362, 1137, 1347
BseNI ACTGG 4 cut(s) 54, 226, 278, 1168
BseRI GAGGAG 1 cut(s) 1002
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 1 cut(s) 1339
BseYI CCCAGC 1 cut(s) 792
BshFI GGCC 4 cut(s) 367, 410, 666, 1166
BshNI GGYRCC 2 cut(s) 482, 1061
BshVI ATCGAT 1 cut(s) 1341
BsiHKAI GWGCWC 3 cut(s) 145, 250, 505
BsiHKCI CYCGRG 1 cut(s) 1322
BsiSI CCGG 5 cut(s) 130, 267, 282, 762, 1163
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
BsmAI GTCTC 3 cut(s) 30, 351, 763
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 4 cut(s) 367, 410, 666, 1166
Bso31I GGTCTC 1 cut(s) 351
BsoBI CYCGRG 1 cut(s) 1322
Bsp119I TTCGAA 1 cut(s) 817
Bsp1286I GDGCHC 4 cut(s) 145, 250, 264, 505
Bsp143I GATC 5 cut(s) 582, 796, 966, 1093, 1189
BspACI CCGC 3 cut(s) 71, 117, 243
BspANI GGCC 4 cut(s) 367, 410, 666, 1166
BspCNI CTCAG 3 cut(s) 361, 1138, 1348
BspDI ATCGAT 1 cut(s) 1341
BspLI GGNNCC 4 cut(s) 163, 411, 484, 1063
BspPI GGATC 5 cut(s) 577, 804, 974, 1088, 1184
BspT104I TTCGAA 1 cut(s) 817
BspT107I GGYRCC 2 cut(s) 482, 1061
BspTNI GGTCTC 1 cut(s) 351
BsrDI GCAATG 2 cut(s) 774, 1297
BsrFI RCCGGY 1 cut(s) 1162
BsrI ACTGG 4 cut(s) 54, 226, 278, 1168
BssAI RCCGGY 1 cut(s) 1162
BssECI CCNNGG 5 cut(s) 265, 412, 506, 762, 886
BssMI GATC 5 cut(s) 582, 796, 966, 1093, 1189
BssNI GRCGYC 1 cut(s) 1062
BssSI CACGAG 1 cut(s) 721
BssT1I CCWWGG 1 cut(s) 886
Bst2BI CACGAG 1 cut(s) 721
Bst2UI CCWGG 4 cut(s) 414, 508, 622, 1080
Bst4CI ACNGT 5 cut(s) 140, 691, 749, 880, 1265
Bst6I CTCTTC 2 cut(s) 375, 779
BstACI GRCGYC 1 cut(s) 1062
BstBAI YACGTR 1 cut(s) 111
BstBI TTCGAA 1 cut(s) 817
BstC8I GCNNGC 3 cut(s) 69, 1164, 1309
BstDEI CTNAG 4 cut(s) 221, 348, 1146, 1356
BstENI CCTNNNNNAGG 1 cut(s) 323
BstH2I RGCGCY 1 cut(s) 1065
BstHHI GCGC 1 cut(s) 1064
BstKTI GATC 5 cut(s) 585, 799, 969, 1096, 1192
BstMAI GTCTC 3 cut(s) 30, 351, 763
BstMBI GATC 5 cut(s) 582, 796, 966, 1093, 1189
BstMWI GCNNNNNNNGC 5 cut(s) 259, 829, 881, 1287, 1327
BstNI CCWGG 4 cut(s) 414, 508, 622, 1080
BstSCI CCNGG 6 cut(s) 265, 412, 506, 620, 761, 1078
BstSFI CTRYAG 2 cut(s) 978, 1129
BstSLI GKGCMC 1 cut(s) 264
BstSNI TACGTA 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 1339
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 1 cut(s) 582
BstXI CCANNNNNNTGG 1 cut(s) 1175
BstYI RGATCY 1 cut(s) 582
Bsu15I ATCGAT 1 cut(s) 1341
Bsu36I CCTNAGG 1 cut(s) 1356
BsuRI GGCC 4 cut(s) 367, 410, 666, 1166
BsuTUI ATCGAT 1 cut(s) 1341
BtgZI GCGATG 1 cut(s) 186
BtsI GCAGTG 3 cut(s) 380, 786, 898
BtsIMutI CAGTG 5 cut(s) 145, 380, 786, 898, 1233
Cac8I GCNNGC 3 cut(s) 69, 1164, 1309
CfoI GCGC 1 cut(s) 1064
Cfr10I RCCGGY 1 cut(s) 1162
Cfr13I GGNCC 3 cut(s) 409, 664, 1165
ClaI ATCGAT 1 cut(s) 1341
Csp6I GTAC 4 cut(s) 112, 571, 1013, 1266
CviAII CATG 2 cut(s) 29, 803
CviQI GTAC 4 cut(s) 112, 571, 1013, 1266
DdeI CTNAG 4 cut(s) 221, 348, 1146, 1356
DinI GGCGCC 1 cut(s) 1063
DpnI GATC 5 cut(s) 584, 798, 968, 1095, 1191
DpnII GATC 5 cut(s) 582, 796, 966, 1093, 1189
DraI TTTAAA 1 cut(s) 202
Eam1104I CTCTTC 2 cut(s) 375, 779
EarI CTCTTC 2 cut(s) 375, 779
EciI GGCGGA 2 cut(s) 106, 258
Eco105I TACGTA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 886
Eco31I GGTCTC 1 cut(s) 351
Eco81I CCTNAGG 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 1322
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO109I RGGNCCY 2 cut(s) 409, 664
EcoRII CCWGG 4 cut(s) 412, 506, 620, 1078
EcoT14I CCWWGG 1 cut(s) 886
EcoT22I ATGCAT 1 cut(s) 235
EgeI GGCGCC 1 cut(s) 1063
EheI GGCGCC 1 cut(s) 1063
ErhI CCWWGG 1 cut(s) 886
FaeI CATG 2 cut(s) 32, 806
FaqI GGGAC 1 cut(s) 527
FatI CATG 2 cut(s) 28, 802
Fnu4HI GCNGC 2 cut(s) 72, 1328
Fsp4HI GCNGC 2 cut(s) 72, 1328
FspBI CTAG 2 cut(s) 887, 1185
GlaI GCGC 1 cut(s) 1063
GluI GCNGC 2 cut(s) 72, 1328
GsaI CCCAGC 1 cut(s) 796
HaeII RGCGCY 1 cut(s) 1065
HaeIII GGCC 4 cut(s) 367, 410, 666, 1166
HapII CCGG 5 cut(s) 130, 267, 282, 762, 1163
HhaI GCGC 1 cut(s) 1064
Hin1I GRCGYC 1 cut(s) 1062
Hin1II CATG 2 cut(s) 32, 806
Hin6I GCGC 1 cut(s) 1062
HinP1I GCGC 1 cut(s) 1062
HindIII AAGCTT 1 cut(s) 286
HinfI GANTC 9 cut(s) 5, 356, 719, 807, 909, 953, 1114, 1297, 1313
HpaII CCGG 5 cut(s) 130, 267, 282, 762, 1163
HphI GGTGA 2 cut(s) 535, 855
Hpy166II GTNNAC 2 cut(s) 728, 1073
Hpy188I TCNGA 4 cut(s) 161, 238, 361, 582
Hpy188III TCNNGA 2 cut(s) 350, 523
Hpy8I GTNNAC 2 cut(s) 728, 1073
HpyAV CCTTC 3 cut(s) 396, 414, 520
HpyCH4III ACNGT 5 cut(s) 140, 691, 749, 880, 1265
HpyCH4IV ACGT 2 cut(s) 110, 1025
HpyCH4V TGCA 8 cut(s) 67, 104, 209, 233, 680, 774, 779, 1209
HpyF10VI GCNNNNNNNGC 5 cut(s) 259, 829, 881, 1287, 1327
HpyF3I CTNAG 4 cut(s) 221, 348, 1146, 1356
HpySE526I ACGT 2 cut(s) 110, 1025
Hsp92I GRCGYC 1 cut(s) 1062
Hsp92II CATG 2 cut(s) 32, 806
HspAI GCGC 1 cut(s) 1062
KasI GGCGCC 1 cut(s) 1061
KroI GCCGGC 1 cut(s) 1162
KroNI GCCGGC 1 cut(s) 1164
Kzo9I GATC 5 cut(s) 582, 796, 966, 1093, 1189
LmnI GCTCC 4 cut(s) 161, 245, 508, 989
Lsp1109I GCAGC 1 cut(s) 1339
LweI GCATC 6 cut(s) 91, 196, 242, 319, 841, 1218
MaeI CTAG 2 cut(s) 887, 1185
MaeII ACGT 2 cut(s) 110, 1025
MalI GATC 5 cut(s) 584, 798, 968, 1095, 1191
MboI GATC 5 cut(s) 582, 796, 966, 1093, 1189
MboII GAAGA 6 cut(s) 362, 365, 755, 796, 941, 1029
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 4 cut(s) 145, 250, 264, 505
MluCI AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
Mly113I GGCGCC 1 cut(s) 1062
MlyI GAGTC 3 cut(s) 365, 713, 1291
MmeI TCCRAC 1 cut(s) 1152
Mph1103I ATGCAT 1 cut(s) 235
MroNI GCCGGC 1 cut(s) 1162
MseI TTAA 8 cut(s) 17, 96, 123, 201, 425, 945, 1383, 1405
MslI CAYNNNNRTG 2 cut(s) 327, 779
MspI CCGG 5 cut(s) 130, 267, 282, 762, 1163
MspR9I CCNGG 6 cut(s) 267, 414, 508, 622, 763, 1080
Mva1269I GAATGC 1 cut(s) 825
MvaI CCWGG 4 cut(s) 414, 508, 622, 1080
MwoI GCNNNNNNNGC 5 cut(s) 259, 829, 881, 1287, 1327
NaeI GCCGGC 1 cut(s) 1164
NarI GGCGCC 1 cut(s) 1062
NciI CCSGG 2 cut(s) 267, 763
NdeII GATC 5 cut(s) 582, 796, 966, 1093, 1189
NgoMIV GCCGGC 1 cut(s) 1162
NlaIII CATG 2 cut(s) 32, 806
NlaIV GGNNCC 4 cut(s) 163, 411, 484, 1063
NmuCI GTSAC 3 cut(s) 541, 934, 1294
NsiI ATGCAT 1 cut(s) 235
NspV TTCGAA 1 cut(s) 817
PctI GAATGC 1 cut(s) 825
PdiI GCCGGC 1 cut(s) 1164
PfeI GAWTC 5 cut(s) 807, 909, 953, 1114, 1313
PfoI TCCNGGA 1 cut(s) 1078
PkrI GCNGC 2 cut(s) 73, 1329
PleI GAGTC 3 cut(s) 364, 713, 1291
PluTI GGCGCC 1 cut(s) 1065
PpsI GAGTC 3 cut(s) 364, 713, 1291
Ppu21I YACGTR 1 cut(s) 111
Psp6I CCWGG 4 cut(s) 412, 506, 620, 1078
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 4 cut(s) 412, 506, 620, 1078
PspN4I GGNNCC 4 cut(s) 163, 411, 484, 1063
PspPI GGNCC 3 cut(s) 409, 664, 1165
PsrI GAACNNNNNNTAC 2 cut(s) 436, 468
PsuI RGATCY 1 cut(s) 582
RsaI GTAC 4 cut(s) 113, 572, 1014, 1267
RsaNI GTAC 4 cut(s) 112, 571, 1013, 1266
RseI CAYNNNNRTG 2 cut(s) 327, 779
SaqAI TTAA 8 cut(s) 17, 96, 123, 201, 425, 945, 1383, 1405
SatI GCNGC 2 cut(s) 72, 1328
Sau3AI GATC 5 cut(s) 582, 796, 966, 1093, 1189
Sau96I GGNCC 3 cut(s) 409, 664, 1165
SchI GAGTC 3 cut(s) 365, 713, 1291
ScrFI CCNGG 6 cut(s) 267, 414, 508, 622, 763, 1080
SduI GDGCHC 4 cut(s) 145, 250, 264, 505
SfaNI GCATC 6 cut(s) 91, 196, 242, 319, 841, 1218
SfcI CTRYAG 2 cut(s) 978, 1129
SfoI GGCGCC 1 cut(s) 1063
SfuI TTCGAA 1 cut(s) 817
SmiMI CAYNNNNRTG 2 cut(s) 327, 779
SnaBI TACGTA 1 cut(s) 111
Sse9I AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
SsiI CCGC 3 cut(s) 71, 117, 243
SspDI GGCGCC 1 cut(s) 1061
SspI AATATT 1 cut(s) 94
SspMI CTAG 2 cut(s) 887, 1185
StyD4I CCNGG 6 cut(s) 265, 412, 506, 620, 761, 1078
StyI CCWWGG 1 cut(s) 886
TaaI ACNGT 5 cut(s) 140, 691, 749, 880, 1265
TaiI ACGT 2 cut(s) 113, 1028
TaqI TCGA 3 cut(s) 736, 817, 1341
TasI AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
TatI WGTACW 2 cut(s) 570, 1265
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 5 cut(s) 807, 909, 953, 1114, 1313
Tru1I TTAA 8 cut(s) 17, 96, 123, 201, 425, 945, 1383, 1405
Tru9I TTAA 8 cut(s) 17, 96, 123, 201, 425, 945, 1383, 1405
TscAI CASTG 5 cut(s) 145, 387, 786, 898, 1240
TseFI GTSAC 3 cut(s) 541, 934, 1294
TseI GCWGC 1 cut(s) 1327
Tsp45I GTSAC 3 cut(s) 541, 934, 1294
TspDTI ATGAA 2 cut(s) 378, 1052
TspRI CASTG 5 cut(s) 145, 387, 786, 898, 1240
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 3 cut(s) 518, 812, 1203
XmaJI CCTAGG 1 cut(s) 886
XspI CTAG 2 cut(s) 887, 1185
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.