Rw1G037600

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002711
Physical Location & Seq
Forward (+)
65265904 .. 65273530
7627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G037600.1

Sequence Viewer

Length: 1428 bp
ATGGACTCAAAATCACTAACAATGTCTTATGCTGCTGCAACCATTGTTATATTTGGATTACTATTCCTACAAGCGGCAGAATGTAGAAACGTGAAGAAGATTAAGGATGGGGTTACGTACTCTGCCATCAAATGCCGGAAGCACAGTGCTCTTTTGACTGATTTCGGTGCCGTTGGCGATGGAAAAACATTAAACACAAAGGCGTTCAAAGCTGCAGTTGATCATCTAAGCAAGTCCGCATCTAAAGGTGGAGCACAGCTCATTGTGCCTCCCGGGAAATGGCTTACCGGGAGCTTCAACCTCACTAGCCATTTTACCCTTTTCCTCCATAAGGATGCTATAATTCTTGGGACTCAGGATGAGTCAGAGTGGCCTATTCTTCCGGTACTGCCTTCATATGGGAGAGGTAGAGATGCCCCCGATGGAAGGTTTAGCAGTCTCATCTTTGGAACAAACCTAACCGACATTATAATTACTGGTAACAATGGCACCCTTGATGGCCAAGGTGCTCCTTGGTGGGACAAGTTCCACAAGGGTCTTTTGAATGTGACTCGTCCATACTTGATTGAGATTATGTACTCTAATCACATCCAAATCTCTAATCTCACTCTGATCAATTCTCCATCTTGGCATGTCCACCCCATTTACAGCAGCTATATAACAATCCAAGGGATTACTATCCTTGCACCAGTTAACGTTCCTAACACAGATGGGATTAACCCAGACTCGTGTTCACAACTTCGAATCGAAGACTGTTACATAGTATCCGGGGATGACTGCATTGCAGTGAAGAGTGGATGGGATCAGTATGGAATCAAAGTTGGAATGCCGACAGAGCATGTTGTGATCAGGAGGCTCACCTGCATTTCACCTAAAAGTGCCACCATTGCCCTAGGCAGTGAAATGTCCGGAGGAATCAGAGACGTTAGAGTTGAGGACGTCACAGCTATTAACACTGAATCCAGCATTAGGATCAAAACCGCTGTTGGAAGAGGGGCTTATGTCAAAGATATCTATGCAAGAAGAGTGACCTTGAAGACAATGAAATATGTTTTTTGGATGACAGGCGCTTATGGTTCGCATCCGAACAATGATTTTGATCCAAATGCACTTCCTGTGATTCAAAACATCAACTTTAAAGAGTTCGTGGCTGAGAATGTTTCCATTCCAGCACAATTAGATGGAATTGCTAATGACCCTTTCAAGGGAATTTGCATGTCTAATGTTTCTATCTCACTTTCTGCGAATCCTACGAAACTTCTGTGGAATTGTACTGGTATTGAGGGAATTACGAGCAATGTGACTCCGAAGGCATGTGATTTGTTACTCCAGCAGCAAAAACCAGTCGATTGTGCTTTCCCCGAGGATAAGTTAGCCATTGAAGGGTTTAACTTGACGACTTGTTTTGCAGGTTTTGCCAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

475

Amino Acids

51.71

Weight (kDa)

7.93

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 146 - 428 8.4e-36 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 470
AarI CACCTGC 1 cut(s) 869
AatII GACGTC 1 cut(s) 942
Acc36I ACCTGC 2 cut(s) 869, 1400
AccB1I GGYRCC 2 cut(s) 167, 488
AccIII TCCGGA 1 cut(s) 908
AciI CCGC 3 cut(s) 74, 237, 981
AclI AACGTT 1 cut(s) 696
AclWI GGATC 3 cut(s) 810, 980, 1094
AcoI YGGCCR 1 cut(s) 499
AcsI RAATTY 1 cut(s) 1209
AcyI GRCGYC 1 cut(s) 939
AfaI GTAC 4 cut(s) 119, 387, 578, 1273
AfiI CCNNNNNNNGG 9 cut(s) 73, 279, 331, 398, 426, 969, 1204, 1205, 1383
AgsI TTSAA 7 cut(s) 208, 298, 544, 1036, 1124, 1204, 1382
AluBI AGCT 5 cut(s) 212, 259, 294, 654, 947
AluI AGCT 5 cut(s) 212, 259, 294, 654, 947
Alw21I GWGCWC 3 cut(s) 151, 256, 511
Alw26I GTCTC 2 cut(s) 443, 915
AlwI GGATC 3 cut(s) 810, 980, 1094
Ama87I CYCGRG 2 cut(s) 272, 1361
Aor13HI TCCGGA 1 cut(s) 908
AoxI GGCC 2 cut(s) 371, 499
ApeKI GCWGC 5 cut(s) 32, 35, 212, 651, 1333
ApoI RAATTY 1 cut(s) 1209
AspA2I CCTAGG 1 cut(s) 892
AspLEI GCGC 1 cut(s) 1070
AsuC2I CCSGG 4 cut(s) 273, 274, 289, 769
AsuHPI GGTGA 2 cut(s) 850, 861
AsuII TTCGAA 1 cut(s) 742
AvaI CYCGRG 2 cut(s) 272, 1361
AvrII CCTAGG 1 cut(s) 892
BalI TGGCCA 1 cut(s) 501
BanI GGYRCC 2 cut(s) 167, 488
BauI CACGAG 1 cut(s) 727
BbsI GAAGAC 2 cut(s) 756, 1043
Bbv12I GWGCWC 3 cut(s) 151, 256, 511
BbvI GCAGC 5 cut(s) 19, 22, 199, 663, 1345
BccI CCATC 9 cut(s) 101, 134, 173, 416, 491, 631, 704, 792, 1175
BceAI ACGGC 1 cut(s) 155
BciVI GTATCC 1 cut(s) 775
BclI TGATCA 3 cut(s) 220, 612, 846
BcnI CCSGG 4 cut(s) 273, 274, 289, 769
BcoDI GTCTC 2 cut(s) 443, 915
BfaI CTAG 2 cut(s) 306, 893
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 1071
BfuAI ACCTGC 2 cut(s) 869, 1400
BfuI GTATCC 1 cut(s) 775
BisI GCNGC 6 cut(s) 33, 36, 75, 213, 652, 1334
BlnI CCTAGG 1 cut(s) 892
BlsI GCNGC 6 cut(s) 34, 37, 76, 214, 653, 1335
Bme1390I CCNGG 4 cut(s) 273, 274, 289, 769
BmeT110I CYCGRG 2 cut(s) 272, 1361
BmiI GGNNCC 2 cut(s) 169, 490
BmrFI CCNGG 4 cut(s) 273, 274, 289, 769
BmsI GCATC 4 cut(s) 248, 325, 403, 1090
BpiI GAAGAC 2 cut(s) 756, 1043
BplI GAGNNNNNCTC 2 cut(s) 243, 275
BpmI CTGGAG 1 cut(s) 1313
Bpu14I TTCGAA 1 cut(s) 742
BpuMI CCSGG 4 cut(s) 273, 274, 289, 769
BsaAI YACGTR 1 cut(s) 117
BsaBI GATNNNNATC 2 cut(s) 677, 1098
BsaHI GRCGYC 1 cut(s) 939
BsaJI CCNNGG 8 cut(s) 272, 502, 512, 667, 768, 892, 1362, 1419
BsaWI WCCGGW 2 cut(s) 382, 908
Bsc4I CCNNNNNNNGG 9 cut(s) 73, 279, 331, 398, 426, 969, 1204, 1205, 1383
Bse1I ACTGG 4 cut(s) 481, 689, 1279, 1343
Bse3DI GCAATG 3 cut(s) 780, 885, 1303
Bse8I GATNNNNATC 2 cut(s) 677, 1098
BseAI TCCGGA 1 cut(s) 908
BseDI CCNNGG 8 cut(s) 272, 502, 512, 667, 768, 892, 1362, 1419
BseGI GGATG 8 cut(s) 112, 340, 364, 588, 778, 803, 1065, 1081
BseJI GATNNNNATC 2 cut(s) 677, 1098
BseLI CCNNNNNNNGG 9 cut(s) 73, 279, 331, 398, 426, 969, 1204, 1205, 1383
BseMI GCAATG 3 cut(s) 780, 885, 1303
BseMII CTCAG 2 cut(s) 368, 1143
BseNI ACTGG 4 cut(s) 481, 689, 1279, 1343
BseXI GCAGC 5 cut(s) 19, 22, 199, 663, 1345
BshFI GGCC 2 cut(s) 373, 501
BshNI GGYRCC 2 cut(s) 167, 488
BsiHKAI GWGCWC 3 cut(s) 151, 256, 511
BsiHKCI CYCGRG 2 cut(s) 272, 1361
BsiSI CCGG 6 cut(s) 136, 273, 288, 383, 768, 909
BslFI GGGAC 2 cut(s) 364, 533
BslI CCNNNNNNNGG 9 cut(s) 73, 279, 331, 398, 426, 969, 1204, 1205, 1383
BsmAI GTCTC 2 cut(s) 443, 915
BsmBI CGTCTC 1 cut(s) 915
BsmFI GGGAC 2 cut(s) 364, 533
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 2 cut(s) 373, 501
BsoBI CYCGRG 2 cut(s) 272, 1361
Bsp119I TTCGAA 1 cut(s) 742
Bsp1286I GDGCHC 3 cut(s) 151, 256, 511
Bsp13I TCCGGA 1 cut(s) 908
Bsp143I GATC 6 cut(s) 220, 612, 802, 846, 972, 1099
BspACI CCGC 3 cut(s) 74, 237, 981
BspANI GGCC 2 cut(s) 373, 501
BspCNI CTCAG 2 cut(s) 367, 1144
BspEI TCCGGA 1 cut(s) 908
BspLI GGNNCC 2 cut(s) 169, 490
BspMAI CTGCAG 1 cut(s) 217
BspMI ACCTGC 2 cut(s) 869, 1400
BspPI GGATC 3 cut(s) 810, 980, 1094
BspT104I TTCGAA 1 cut(s) 742
BspT107I GGYRCC 2 cut(s) 167, 488
BsrDI GCAATG 3 cut(s) 780, 885, 1303
BsrI ACTGG 4 cut(s) 481, 689, 1279, 1343
BssECI CCNNGG 8 cut(s) 272, 502, 512, 667, 768, 892, 1362, 1419
BssMI GATC 6 cut(s) 220, 612, 802, 846, 972, 1099
BssNI GRCGYC 1 cut(s) 939
BssSI CACGAG 1 cut(s) 727
BssT1I CCWWGG 5 cut(s) 502, 512, 667, 892, 1419
Bst2BI CACGAG 1 cut(s) 727
Bst4CI ACNGT 2 cut(s) 146, 755
Bst6I CTCTTC 3 cut(s) 785, 985, 1018
BstACI GRCGYC 1 cut(s) 939
BstAPI GCANNNNNTGC 1 cut(s) 1415
BstBAI YACGTR 1 cut(s) 117
BstBI TTCGAA 1 cut(s) 742
BstDEI CTNAG 3 cut(s) 227, 354, 1152
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 8 cut(s) 112, 340, 364, 588, 778, 803, 1065, 1081
BstH2I RGCGCY 1 cut(s) 1071
BstHHI GCGC 1 cut(s) 1070
BstKTI GATC 6 cut(s) 223, 615, 805, 849, 975, 1102
BstMAI GTCTC 2 cut(s) 443, 915
BstMBI GATC 6 cut(s) 220, 612, 802, 846, 972, 1099
BstMWI GCNNNNNNNGC 5 cut(s) 209, 265, 835, 887, 1415
BstNSI RCATGY 4 cut(s) 635, 842, 1219, 1317
BstSCI CCNGG 4 cut(s) 271, 272, 287, 767
BstSFI CTRYAG 1 cut(s) 213
BstSNI TACGTA 1 cut(s) 117
BstV1I GCAGC 5 cut(s) 19, 22, 199, 663, 1345
BstV2I GAAGAC 2 cut(s) 756, 1043
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 2 cut(s) 373, 501
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 8 cut(s) 112, 340, 364, 588, 778, 803, 1065, 1081
BtsI GCAGTG 2 cut(s) 792, 904
BtsIMutI CAGTG 4 cut(s) 151, 792, 904, 954
BveI ACCTGC 2 cut(s) 869, 1400
CfoI GCGC 1 cut(s) 1070
Cfr9I CCCGGG 1 cut(s) 272
Csp6I GTAC 4 cut(s) 118, 386, 577, 1272
CviAII CATG 4 cut(s) 632, 839, 1216, 1314
CviQI GTAC 4 cut(s) 118, 386, 577, 1272
DdeI CTNAG 3 cut(s) 227, 354, 1152
DpnI GATC 6 cut(s) 222, 614, 804, 848, 974, 1101
DpnII GATC 6 cut(s) 220, 612, 802, 846, 972, 1099
DraI TTTAAA 1 cut(s) 1138
EaeI YGGCCR 1 cut(s) 499
Eam1104I CTCTTC 3 cut(s) 785, 985, 1018
EarI CTCTTC 3 cut(s) 785, 985, 1018
Eco105I TACGTA 1 cut(s) 117
Eco130I CCWWGG 5 cut(s) 502, 512, 667, 892, 1419
Eco32I GATATC 1 cut(s) 1012
Eco88I CYCGRG 2 cut(s) 272, 1361
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoRV GATATC 1 cut(s) 1012
EcoT14I CCWWGG 5 cut(s) 502, 512, 667, 892, 1419
ErhI CCWWGG 5 cut(s) 502, 512, 667, 892, 1419
Esp3I CGTCTC 1 cut(s) 915
FaeI CATG 4 cut(s) 635, 842, 1219, 1317
FalI AAGNNNNNCTT 2 cut(s) 982, 1014
FaqI GGGAC 2 cut(s) 364, 533
FatI CATG 4 cut(s) 631, 838, 1215, 1313
FauNDI CATATG 1 cut(s) 397
FbaI TGATCA 3 cut(s) 220, 612, 846
Fnu4HI GCNGC 6 cut(s) 33, 36, 75, 213, 652, 1334
FokI GGATG 8 cut(s) 119, 347, 371, 575, 785, 810, 1068, 1072
Fsp4HI GCNGC 6 cut(s) 33, 36, 75, 213, 652, 1334
FspBI CTAG 2 cut(s) 306, 893
GlaI GCGC 1 cut(s) 1069
GluI GCNGC 6 cut(s) 33, 36, 75, 213, 652, 1334
GsuI CTGGAG 1 cut(s) 1313
HaeII RGCGCY 1 cut(s) 1071
HaeIII GGCC 2 cut(s) 373, 501
HapII CCGG 6 cut(s) 136, 273, 288, 383, 768, 909
HhaI GCGC 1 cut(s) 1070
Hin1I GRCGYC 1 cut(s) 939
Hin1II CATG 4 cut(s) 635, 842, 1219, 1317
Hin6I GCGC 1 cut(s) 1068
HinP1I GCGC 1 cut(s) 1068
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HpaI GTTAAC 1 cut(s) 694
HpaII CCGG 6 cut(s) 136, 273, 288, 383, 768, 909
HphI GGTGA 2 cut(s) 850, 861
Hpy166II GTNNAC 3 cut(s) 637, 694, 734
Hpy188I TCNGA 5 cut(s) 367, 612, 920, 1086, 1308
Hpy188III TCNNGA 3 cut(s) 356, 850, 909
Hpy8I GTNNAC 3 cut(s) 637, 694, 734
HpyAV CCTTC 4 cut(s) 402, 420, 1303, 1376
HpyCH4III ACNGT 2 cut(s) 146, 755
HpyCH4IV ACGT 5 cut(s) 90, 116, 696, 924, 939
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 265, 835, 887, 1415
HpyF3I CTNAG 3 cut(s) 227, 354, 1152
HpySE526I ACGT 5 cut(s) 90, 116, 696, 924, 939
Hsp92I GRCGYC 1 cut(s) 939
Hsp92II CATG 4 cut(s) 635, 842, 1219, 1317
HspAI GCGC 1 cut(s) 1068
Kpn2I TCCGGA 1 cut(s) 908
Ksp22I TGATCA 3 cut(s) 220, 612, 846
KspAI GTTAAC 1 cut(s) 694
Kzo9I GATC 6 cut(s) 220, 612, 802, 846, 972, 1099
LmnI GCTCC 3 cut(s) 251, 291, 514
Lsp1109I GCAGC 5 cut(s) 19, 22, 199, 663, 1345
LweI GCATC 4 cut(s) 248, 325, 403, 1090
MaeI CTAG 2 cut(s) 306, 893
MaeII ACGT 5 cut(s) 90, 116, 696, 924, 939
MaeIII GTNAC 8 cut(s) 112, 479, 547, 755, 940, 1027, 1300, 1323
MalI GATC 6 cut(s) 222, 614, 804, 848, 974, 1101
MboI GATC 6 cut(s) 220, 612, 802, 846, 972, 1099
MboII GAAGA 8 cut(s) 106, 109, 371, 761, 802, 1002, 1035, 1048
MhlI GDGCHC 3 cut(s) 151, 256, 511
MlsI TGGCCA 1 cut(s) 501
MluCI AATT 8 cut(s) 342, 471, 616, 1175, 1185, 1209, 1267, 1287
MluNI TGGCCA 1 cut(s) 501
MlyI GAGTC 5 cut(s) 346, 371, 544, 719, 1297
MmeI TCCRAC 2 cut(s) 802, 967
Mox20I TGGCCA 1 cut(s) 501
MroI TCCGGA 1 cut(s) 908
MscI TGGCCA 1 cut(s) 501
MseI TTAA 7 cut(s) 102, 191, 693, 717, 951, 1137, 1389
MslI CAYNNNNRTG 2 cut(s) 333, 785
Msp20I TGGCCA 1 cut(s) 501
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 6 cut(s) 136, 273, 288, 383, 768, 909
MspR9I CCNGG 4 cut(s) 273, 274, 289, 769
Mva1269I GAATGC 1 cut(s) 831
MwoI GCNNNNNNNGC 5 cut(s) 209, 265, 835, 887, 1415
NciI CCSGG 4 cut(s) 273, 274, 289, 769
NdeI CATATG 1 cut(s) 397
NdeII GATC 6 cut(s) 220, 612, 802, 846, 972, 1099
NlaIII CATG 4 cut(s) 635, 842, 1219, 1317
NlaIV GGNNCC 2 cut(s) 169, 490
NmuCI GTSAC 4 cut(s) 547, 940, 1027, 1300
NspI RCATGY 4 cut(s) 635, 842, 1219, 1317
NspV TTCGAA 1 cut(s) 742
PaqCI CACCTGC 1 cut(s) 869
PctI GAATGC 1 cut(s) 831
PfeI GAWTC 6 cut(s) 744, 813, 915, 959, 1120, 1246
PkrI GCNGC 6 cut(s) 34, 37, 76, 214, 653, 1335
PleI GAGTC 5 cut(s) 346, 370, 544, 719, 1297
PpsI GAGTC 5 cut(s) 346, 370, 544, 719, 1297
Ppu21I YACGTR 1 cut(s) 117
PsiI TTATAA 1 cut(s) 470
Psp1406I AACGTT 1 cut(s) 696
PspN4I GGNNCC 2 cut(s) 169, 490
PstI CTGCAG 1 cut(s) 217
RsaI GTAC 4 cut(s) 119, 387, 578, 1273
RsaNI GTAC 4 cut(s) 118, 386, 577, 1272
RseI CAYNNNNRTG 2 cut(s) 333, 785
SaqAI TTAA 7 cut(s) 102, 191, 693, 717, 951, 1137, 1389
SatI GCNGC 6 cut(s) 33, 36, 75, 213, 652, 1334
Sau3AI GATC 6 cut(s) 220, 612, 802, 846, 972, 1099
SchI GAGTC 5 cut(s) 346, 371, 544, 719, 1297
ScrFI CCNGG 4 cut(s) 273, 274, 289, 769
SduI GDGCHC 3 cut(s) 151, 256, 511
SfaNI GCATC 4 cut(s) 248, 325, 403, 1090
SfcI CTRYAG 1 cut(s) 213
SfuI TTCGAA 1 cut(s) 742
SmaI CCCGGG 1 cut(s) 274
SmiMI CAYNNNNRTG 2 cut(s) 333, 785
SnaBI TACGTA 1 cut(s) 117
Sse9I AATT 8 cut(s) 342, 471, 616, 1175, 1185, 1209, 1267, 1287
SsiI CCGC 3 cut(s) 74, 237, 981
SspMI CTAG 2 cut(s) 306, 893
StyD4I CCNGG 4 cut(s) 271, 272, 287, 767
StyI CCWWGG 5 cut(s) 502, 512, 667, 892, 1419
TaaI ACNGT 2 cut(s) 146, 755
TaiI ACGT 5 cut(s) 93, 119, 699, 927, 942
TaqI TCGA 3 cut(s) 742, 747, 1347
TasI AATT 8 cut(s) 342, 471, 616, 1175, 1185, 1209, 1267, 1287
TatI WGTACW 2 cut(s) 576, 1271
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 6 cut(s) 744, 813, 915, 959, 1120, 1246
Tru1I TTAA 7 cut(s) 102, 191, 693, 717, 951, 1137, 1389
Tru9I TTAA 7 cut(s) 102, 191, 693, 717, 951, 1137, 1389
TscAI CASTG 4 cut(s) 151, 792, 904, 961
TseFI GTSAC 4 cut(s) 547, 940, 1027, 1300
TseI GCWGC 5 cut(s) 32, 35, 212, 651, 1333
Tsp45I GTSAC 4 cut(s) 547, 940, 1027, 1300
TspDTI ATGAA 2 cut(s) 384, 1058
TspMI CCCGGG 1 cut(s) 272
TspRI CASTG 4 cut(s) 151, 792, 904, 961
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 1 cut(s) 1209
XceI RCATGY 4 cut(s) 635, 842, 1219, 1317
XmaI CCCGGG 1 cut(s) 272
XmaJI CCTAGG 1 cut(s) 892
XspI CTAG 2 cut(s) 306, 893
ZraI GACGTC 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.