AT2G23900

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002711
Physical Location & Seq
Forward (+)
10174515 .. 10176858
2344 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G23900.1

Sequence Viewer

Length: 1434 bp
ATGGAGTTCTCTATCAATCCTTCAACTCTAAAGATGATTCGGACAAGTTTTTGTATATTAGTAGTTTTGGCAATTAGTAGTTTTTCGATGATGGAAGCTCGGGATCTAGCTAGCAAAGGAAAGACAAATATTGAATATATGGCGTTGAATTGTCGAAAACATACCGCAGTTTTGACAGAATTTGGAGCAGTTGGTGATGGAAAGACCTCGAACACAAAAGCGTTCAAAGAAGCCATAACCAAGCTTGCTCCAAAGGCAGCCGACGGTGGAGTGCAACTCATTGTTCCACCAGGAAAATGGCTAACCGGAAGTTTCAACCTCACCAGCCATTTTACTTTGTTCATCCAAAAAGGTGCAACTATTCTTGCTTCTCAGGATGAATCTGAATATCCAGTGGTTGCACCTTTGCCATCATACGGACAAGGAAGAGATGCAGCAGGACCAACATTTGCTAGTCTAATCTCTGGCACAAACCTAACTGATGTTGTTATCACCGGTAACAACGGAACGATCAACGGGCAAGGAAAGTACTGGTGGGTGAAGTATCGCAGTGGTGGATTTAAGGGGATCACAAGACCTTACACAATCGAGATCATCTTCTCTCAAAACGTTCAGATCTCGAACATCACAATCATCGATTCGCCTGCATGGAATATTCATCCTGTATATTGCAACAATGTCATCGTCAAAGGCGTTACCATTCTTGCTCCTATCGATTCTCCTAATACCGATGGAATCAACCCTGATTCATGCACCAACACATTGATAGAAGACTGTTACGTAGTCTCGGGAGACGATTGCATCGCCGTGAAGAGCGGTTGGGATCAGTTTGGTATCAAAGTCGGCATGCCAACTCAACAATTGTCTATCCGACGGCTCACATGCATCTCTCCCGACAGCGCTGGAATAGCACTCGGAAGTGAAATGTCCGGTGGAATCAAAGACGTTAGAATCGAAGACATTACATTACTTCAGACACAATCCGCTATCCGAATCAAAACCGCGGTTGGTCGTGGTGGTTATGTTAAGGATATTTTCGCCAGGAGGTTCACAATGAAAACAATGAAATACGTTTTCTGGATGAGCGGTGCTTATAACCAACACCCCGCTTCCGGTTTTGATCCCAAGGCTATGCCCGTGATTACGAATATCAACTACCGTGACATGACCGCGGATAATGTCACGCAACCTGCGAGGCTCGATGGGTTCAAGAACGATCCTTTCACAAAGATATGTATGTCGAATATAAAGATTGATTTGGCTGCTGAGCCGAAGAAGTTGTTGTGGAATTGTACAAGCATCTCTGGAGTTTCGAGCAAGGTTACACCTAAACCGTGTAGTTTGTTGCCGGAGAAAGGAGCACCGGTGGACTGTGCTTTTCCGGTTGATAAGATTCCTATTGAATCTGTTGTCCTGAACAAATGCTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

477

Amino Acids

51.64

Weight (kDa)

8.78

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 146 - 434 3.2e-41 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1095
Acc36I ACCTGC 1 cut(s) 1198
AccBSI CCGCTC 2 cut(s) 816, 1086
AccII CGCG 2 cut(s) 1004, 1172
AciI CCGC 9 cut(s) 165, 816, 984, 1002, 1004, 1086, 1107, 1170, 1172
AclI AACGTT 1 cut(s) 609
AclWI GGATC 5 cut(s) 111, 575, 831, 1115, 1211
AcsI RAATTY 1 cut(s) 179
AcuI CTGAAG 1 cut(s) 956
AfaI GTAC 2 cut(s) 530, 1294
AfeI AGCGCT 1 cut(s) 901
AfiI CCNNNNNNNGG 3 cut(s) 416, 1112, 1355
AgeI ACCGGT 2 cut(s) 494, 1363
AgsI TTSAA 7 cut(s) 24, 134, 148, 226, 316, 1210, 1403
AjnI CCWGG 2 cut(s) 289, 1040
AluBI AGCT 3 cut(s) 98, 110, 244
AluI AGCT 3 cut(s) 98, 110, 244
Alw21I GWGCWC 1 cut(s) 1363
Alw26I GTCTC 2 cut(s) 786, 790
AlwI GGATC 5 cut(s) 111, 575, 831, 1115, 1211
Ama87I CYCGRG 2 cut(s) 99, 787
Aor51HI AGCGCT 1 cut(s) 901
ApeKI GCWGC 3 cut(s) 257, 434, 1262
ApoI RAATTY 1 cut(s) 179
AsiGI ACCGGT 2 cut(s) 494, 1363
AspLEI GCGC 1 cut(s) 902
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 4 cut(s) 206, 313, 484, 550
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 2 cut(s) 99, 787
AvaII GGWCC 1 cut(s) 440
BbsI GAAGAC 2 cut(s) 777, 963
Bbv12I GWGCWC 1 cut(s) 1363
BbvI GCAGC 3 cut(s) 269, 446, 1249
BccI CCATC 5 cut(s) 85, 191, 418, 725, 1196
BceAI ACGGC 2 cut(s) 791, 890
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 2 cut(s) 291, 1042
BcoDI GTCTC 2 cut(s) 786, 790
BfaI CTAG 3 cut(s) 107, 111, 453
BfoI RGCGCY 1 cut(s) 903
BfuAI ACCTGC 1 cut(s) 1198
BglII AGATCT 1 cut(s) 615
BisI GCNGC 3 cut(s) 258, 435, 1263
BlpI GCTNAGC 1 cut(s) 1266
BlsI GCNGC 3 cut(s) 259, 436, 1264
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 291, 1042
Bme18I GGWCC 1 cut(s) 440
BmeT110I CYCGRG 2 cut(s) 99, 787
BmgT120I GGNCC 1 cut(s) 440
BmrFI CCNGG 2 cut(s) 291, 1042
BmsI GCATC 4 cut(s) 421, 810, 894, 1308
BmtI GCTAGC 1 cut(s) 114
BpiI GAAGAC 2 cut(s) 777, 963
BplI GAGNNNNNCTC 2 cut(s) 261, 293
BpmI CTGGAG 1 cut(s) 1326
Bpu1102I GCTNAGC 1 cut(s) 1266
Bsa29I ATCGAT 2 cut(s) 636, 714
BsaAI YACGTR 1 cut(s) 781
BsaJI CCNNGG 3 cut(s) 1002, 1125, 1170
BsaWI WCCGGW 6 cut(s) 305, 494, 929, 1112, 1363, 1381
BsaXI ACNNNNNCTCC 2 cut(s) 1350, 1380
Bsc4I CCNNNNNNNGG 3 cut(s) 416, 1112, 1355
Bse118I RCCGGY 2 cut(s) 494, 1363
Bse1I ACTGG 2 cut(s) 392, 536
BseBI CCWGG 2 cut(s) 291, 1042
BseCI ATCGAT 2 cut(s) 636, 714
BseDI CCNNGG 3 cut(s) 1002, 1125, 1170
BseGI GGATG 4 cut(s) 342, 382, 658, 1086
BseLI CCNNNNNNNGG 3 cut(s) 416, 1112, 1355
BseMII CTCAG 2 cut(s) 386, 1257
BseNI ACTGG 2 cut(s) 392, 536
BseXI GCAGC 3 cut(s) 269, 446, 1249
Bsh1236I CGCG 2 cut(s) 1004, 1172
BshTI ACCGGT 2 cut(s) 494, 1363
BshVI ATCGAT 2 cut(s) 636, 714
BsiHKAI GWGCWC 1 cut(s) 1363
BsiHKCI CYCGRG 2 cut(s) 99, 787
BsiSI CCGG 7 cut(s) 306, 495, 930, 1113, 1349, 1364, 1382
BslI CCNNNNNNNGG 3 cut(s) 416, 1112, 1355
BsmAI GTCTC 2 cut(s) 786, 790
BsmBI CGTCTC 1 cut(s) 786
BsoBI CYCGRG 2 cut(s) 99, 787
Bsp1286I GDGCHC 1 cut(s) 1363
Bsp1407I TGTACA 1 cut(s) 1292
Bsp143I GATC 8 cut(s) 103, 510, 567, 591, 615, 823, 1120, 1216
Bsp1720I GCTNAGC 1 cut(s) 1266
BspACI CCGC 9 cut(s) 165, 816, 984, 1002, 1004, 1086, 1107, 1170, 1172
BspCNI CTCAG 2 cut(s) 385, 1258
BspDI ATCGAT 2 cut(s) 636, 714
BspFNI CGCG 2 cut(s) 1004, 1172
BspMI ACCTGC 1 cut(s) 1198
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 5 cut(s) 111, 575, 831, 1115, 1211
BspQI GCTCTTC 1 cut(s) 806
BsrBI CCGCTC 2 cut(s) 816, 1086
BsrFI RCCGGY 2 cut(s) 494, 1363
BsrGI TGTACA 1 cut(s) 1292
BsrI ACTGG 2 cut(s) 392, 536
BssAI RCCGGY 2 cut(s) 494, 1363
BssECI CCNNGG 3 cut(s) 1002, 1125, 1170
BssMI GATC 8 cut(s) 103, 510, 567, 591, 615, 823, 1120, 1216
BssT1I CCWWGG 1 cut(s) 1125
Bst2UI CCWGG 2 cut(s) 291, 1042
Bst4CI ACNGT 5 cut(s) 266, 776, 1160, 1335, 1373
Bst6I CTCTTC 2 cut(s) 421, 806
BstAUI TGTACA 1 cut(s) 1292
BstBAI YACGTR 1 cut(s) 781
BstC8I GCNNGC 4 cut(s) 112, 246, 645, 848
BstDEI CTNAG 3 cut(s) 372, 1266, 1431
BstDSI CCRYGG 2 cut(s) 1002, 1170
BstF5I GGATG 4 cut(s) 342, 382, 658, 1086
BstFNI CGCG 2 cut(s) 1004, 1172
BstH2I RGCGCY 1 cut(s) 903
BstHHI GCGC 1 cut(s) 902
BstKTI GATC 8 cut(s) 106, 513, 570, 594, 618, 826, 1123, 1219
BstMAI GTCTC 2 cut(s) 786, 790
BstMBI GATC 8 cut(s) 103, 510, 567, 591, 615, 823, 1120, 1216
BstMWI GCNNNNNNNGC 2 cut(s) 254, 908
BstNI CCWGG 2 cut(s) 291, 1042
BstNSI RCATGY 2 cut(s) 850, 885
BstSCI CCNGG 2 cut(s) 289, 1040
BstSNI TACGTA 1 cut(s) 781
BstUI CGCG 2 cut(s) 1004, 1172
BstV1I GCAGC 3 cut(s) 269, 446, 1249
BstV2I GAAGAC 2 cut(s) 777, 963
BstX2I RGATCY 2 cut(s) 103, 615
BstXI CCANNNNNNTGG 1 cut(s) 297
BstYI RGATCY 2 cut(s) 103, 615
Bsu15I ATCGAT 2 cut(s) 636, 714
BsuTUI ATCGAT 2 cut(s) 636, 714
BtgI CCRYGG 2 cut(s) 1002, 1170
BtgZI GCGATG 1 cut(s) 787
BtsCI GGATG 4 cut(s) 342, 382, 658, 1086
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 2 cut(s) 399, 556
BveI ACCTGC 1 cut(s) 1198
Cac8I GCNNGC 4 cut(s) 112, 246, 645, 848
CfoI GCGC 1 cut(s) 902
Cfr10I RCCGGY 2 cut(s) 494, 1363
Cfr13I GGNCC 1 cut(s) 440
Cfr42I CCGCGG 2 cut(s) 1005, 1173
ClaI ATCGAT 2 cut(s) 636, 714
Csp6I GTAC 2 cut(s) 529, 1293
CspAI ACCGGT 2 cut(s) 494, 1363
CviAII CATG 5 cut(s) 648, 750, 847, 882, 1165
CviQI GTAC 2 cut(s) 529, 1293
DdeI CTNAG 3 cut(s) 372, 1266, 1431
DpnI GATC 8 cut(s) 105, 512, 569, 593, 617, 825, 1122, 1218
DpnII GATC 8 cut(s) 103, 510, 567, 591, 615, 823, 1120, 1216
Eam1104I CTCTTC 2 cut(s) 421, 806
EarI CTCTTC 2 cut(s) 421, 806
Eco105I TACGTA 1 cut(s) 781
Eco130I CCWWGG 1 cut(s) 1125
Eco47I GGWCC 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 901
Eco57I CTGAAG 1 cut(s) 956
Eco88I CYCGRG 2 cut(s) 99, 787
EcoRII CCWGG 2 cut(s) 289, 1040
EcoT14I CCWWGG 1 cut(s) 1125
EcoT22I ATGCAT 1 cut(s) 887
ErhI CCWWGG 1 cut(s) 1125
Esp3I CGTCTC 1 cut(s) 786
FaeI CATG 5 cut(s) 651, 753, 850, 885, 1168
FatI CATG 5 cut(s) 647, 749, 846, 881, 1164
FauI CCCGC 1 cut(s) 1114
Fnu4HI GCNGC 3 cut(s) 258, 435, 1263
FokI GGATG 4 cut(s) 329, 389, 645, 1093
Fsp4HI GCNGC 3 cut(s) 258, 435, 1263
FspBI CTAG 3 cut(s) 107, 111, 453
GlaI GCGC 1 cut(s) 901
GluI GCNGC 3 cut(s) 258, 435, 1263
GsuI CTGGAG 1 cut(s) 1326
HaeII RGCGCY 1 cut(s) 903
HapII CCGG 7 cut(s) 306, 495, 930, 1113, 1349, 1364, 1382
HhaI GCGC 1 cut(s) 902
Hin1II CATG 5 cut(s) 651, 753, 850, 885, 1168
Hin6I GCGC 1 cut(s) 900
HinP1I GCGC 1 cut(s) 900
HindIII AAGCTT 1 cut(s) 242
HpaII CCGG 7 cut(s) 306, 495, 930, 1113, 1349, 1364, 1382
HphI GGTGA 4 cut(s) 206, 313, 484, 550
Hpy166II GTNNAC 2 cut(s) 1050, 1369
Hpy188I TCNGA 7 cut(s) 42, 385, 615, 872, 917, 975, 992
Hpy8I GTNNAC 2 cut(s) 1050, 1369
Hpy99I CGWCG 2 cut(s) 266, 876
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 5 cut(s) 266, 776, 1160, 1335, 1373
HpyCH4IV ACGT 4 cut(s) 609, 780, 945, 1071
HpyCH4V TGCA 9 cut(s) 274, 356, 401, 434, 647, 672, 753, 801, 885
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 908
HpyF3I CTNAG 3 cut(s) 372, 1266, 1431
HpySE526I ACGT 4 cut(s) 609, 780, 945, 1071
Hsp92II CATG 5 cut(s) 651, 753, 850, 885, 1168
HspAI GCGC 1 cut(s) 900
KspI CCGCGG 2 cut(s) 1005, 1173
Kzo9I GATC 8 cut(s) 103, 510, 567, 591, 615, 823, 1120, 1216
LguI GCTCTTC 1 cut(s) 806
LmnI GCTCC 4 cut(s) 185, 253, 712, 1358
Lsp1109I GCAGC 3 cut(s) 269, 446, 1249
LweI GCATC 4 cut(s) 421, 810, 894, 1308
MaeI CTAG 3 cut(s) 107, 111, 453
MaeII ACGT 4 cut(s) 609, 780, 945, 1071
MaeIII GTNAC 6 cut(s) 497, 694, 776, 1160, 1180, 1321
MalI GATC 8 cut(s) 105, 512, 569, 593, 617, 825, 1122, 1218
MbiI CCGCTC 2 cut(s) 816, 1086
MboI GATC 8 cut(s) 103, 510, 567, 591, 615, 823, 1120, 1216
MboII GAAGA 6 cut(s) 438, 589, 782, 823, 968, 1285
MfeI CAATTG 1 cut(s) 860
MflI RGATCY 2 cut(s) 103, 615
MhlI GDGCHC 1 cut(s) 1363
MluCI AATT 5 cut(s) 72, 148, 179, 860, 1288
MmeI TCCRAC 1 cut(s) 895
MnlI CCTC 4 cut(s) 217, 329, 1038, 1188
Mph1103I ATGCAT 1 cut(s) 887
MseI TTAA 2 cut(s) 561, 1026
MslI CAYNNNNRTG 1 cut(s) 806
MspA1I CMGCKG 2 cut(s) 1004, 1172
MspI CCGG 7 cut(s) 306, 495, 930, 1113, 1349, 1364, 1382
MspR9I CCNGG 2 cut(s) 291, 1042
MunI CAATTG 1 cut(s) 860
MvaI CCWGG 2 cut(s) 291, 1042
MvnI CGCG 2 cut(s) 1004, 1172
MwoI GCNNNNNNNGC 2 cut(s) 254, 908
NdeII GATC 8 cut(s) 103, 510, 567, 591, 615, 823, 1120, 1216
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 5 cut(s) 651, 753, 850, 885, 1168
NmuCI GTSAC 2 cut(s) 1160, 1180
NsiI ATGCAT 1 cut(s) 887
NspI RCATGY 2 cut(s) 850, 885
PaeI GCATGC 1 cut(s) 850
PciSI GCTCTTC 1 cut(s) 806
PcsI WCGNNNNNNNCGW 3 cut(s) 690, 951, 1190
PinAI ACCGGT 2 cut(s) 494, 1363
PkrI GCNGC 3 cut(s) 259, 436, 1264
Ppu21I YACGTR 1 cut(s) 781
PsiI TTATAA 1 cut(s) 1095
Psp1406I AACGTT 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 289, 1040
PspGI CCWGG 2 cut(s) 289, 1040
PspPI GGNCC 1 cut(s) 440
PsuI RGATCY 2 cut(s) 103, 615
RsaI GTAC 2 cut(s) 530, 1294
RsaNI GTAC 2 cut(s) 529, 1293
RseI CAYNNNNRTG 1 cut(s) 806
SacII CCGCGG 2 cut(s) 1005, 1173
SapI GCTCTTC 1 cut(s) 806
SaqAI TTAA 2 cut(s) 561, 1026
SatI GCNGC 3 cut(s) 258, 435, 1263
Sau3AI GATC 8 cut(s) 103, 510, 567, 591, 615, 823, 1120, 1216
Sau96I GGNCC 1 cut(s) 440
ScaI AGTACT 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 291, 1042
SduI GDGCHC 1 cut(s) 1363
SfaNI GCATC 4 cut(s) 421, 810, 894, 1308
Sfr303I CCGCGG 2 cut(s) 1005, 1173
SgrAI CRCCGGYG 1 cut(s) 1363
SgrBI CCGCGG 2 cut(s) 1005, 1173
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 806
SnaBI TACGTA 1 cut(s) 781
SphI GCATGC 1 cut(s) 850
Sse9I AATT 5 cut(s) 72, 148, 179, 860, 1288
SsiI CCGC 9 cut(s) 165, 816, 984, 1002, 1004, 1086, 1107, 1170, 1172
SspI AATATT 2 cut(s) 130, 655
SspMI CTAG 3 cut(s) 107, 111, 453
StyD4I CCNGG 2 cut(s) 289, 1040
StyI CCWWGG 1 cut(s) 1125
TaaI ACNGT 5 cut(s) 266, 776, 1160, 1335, 1373
TaiI ACGT 4 cut(s) 612, 783, 948, 1074
TasI AATT 5 cut(s) 72, 148, 179, 860, 1288
TatI WGTACW 2 cut(s) 528, 1292
Tru1I TTAA 2 cut(s) 561, 1026
Tru9I TTAA 2 cut(s) 561, 1026
TscAI CASTG 2 cut(s) 399, 556
TseFI GTSAC 2 cut(s) 1160, 1180
TseI GCWGC 3 cut(s) 257, 434, 1262
Tsp45I GTSAC 2 cut(s) 1160, 1180
TspDTI ATGAA 6 cut(s) 331, 393, 647, 738, 1070, 1079
TspGWI ACGGA 2 cut(s) 432, 519
TspRI CASTG 2 cut(s) 399, 556
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 179
XceI RCATGY 2 cut(s) 850, 885
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XspI CTAG 3 cut(s) 107, 111, 453
ZrmI AGTACT 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.