Rh4AG189700

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002711
Physical Location & Seq
Forward (+)
47068459 .. 47071005
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG189700.1

Sequence Viewer

Length: 1407 bp
ATGGACTCAAACTCTTCAACAATGTCTCATGTTGCTACTATTGTTATACTGGGATTACTATTCCTACAAGCGGCAGAATGTAGAATACAGAATATTAAGGATGTAGTTACGTACTCCGCCATTAACTGCCGGAAGCACAGTGCTCTTTTGACTGATTTCGGAGCTGTTGGCGATGGAAAGACATTGAACACAAAGGCCTTTAAAGATGCAGTTGATAAACTAAGCCAGTATGCATCTGATGGTGGAGCACAGCTAATTGTGCCCCCCGGTAACTGGCTTACCGGGAGCTTCAACCTCACTAGCCACTTTACCCTTTTCCTCCACAAGGATGCTGTTATTCTTGGGTCTCAGGATGAGTCCGAGTGGCCTCTTCTTCCAGCACTGCCTTCATACGGGCAAGGGAGAGAGGGCCCTGGTGGAAGGTTAAGCAGTCTGATCACTGGAACAAACCTTACCGACGTCGTAATTACCGGTAACAATGGGACCATTGATGGGCAAGGCGCTCCCTGGTGGGAAAAATTCAAGAAGGGTCTTTTGAATGTCACCCGACCATACTTGGTTGAGATTATGTACTCTAATGAGATCCAAATCTCCAATCTCACTTTTACGAATTCTCCATCCTGGCACATCCATCCCATTTACAGCAGTAACATTACCATACAAGGCCTCACTATCCTTGCACCAGTTAGCGTTCCTAACACAGATGGGATTAACCCTGATTCGTGTTCACAAACTCGAATTGAAGACTGTTACATAGTCTCCGGGGATGACTGCATTGCAGTGAAGAGTGGCTGGGATCAGTATGGAATCAAATTTGGAATGCCAACCGAGCATCTTGTTATCAGGAGGCTTACCTGTATTTCACCTAAAAGTGCTACTGTTGCCCTAGGCAGTGAAATGTCTGGTGGAATCAGGGATGTAAGAGTTGAAGATGTCACAGCCATTCACACCGAATCCAGCATTAGGATCAAAACAGCTGTAGGCAGAGGGGCTTATGTCAAAGACATTTATGTACGGAGATTGAAGTTGAAGACTATGAAATACGTTTTTTGGATGACAGGCGGCTATGGTTCACATCCAGGCGAGGGCTTTGATCCAAAGGCACTTCCTGAGATTCAAAACATCAACTATAGAGAGGTTGTTGCTGAGAATGTTACAATGCCTGCCCAGTTGGATGGAATTGCTAGTGATCCTTTCAAGGGAATTTGCATCTCTAATGTGAGTATCTCACTCACTGAAAAGCCAAAGAAATTGTTGTGGAACTGTACTGATGTTGAGGGCATTACGAGCAATGTGACTCCAAAGGCTTGCGATTTGTTGCCCGAGCAGCAAAAACCAATCGATTGTGCTTTCCCTGAGGATAAGTTAGCCATTGAGGGCGTTAAGCTGACCACTTGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

468

Amino Acids

50.86

Weight (kDa)

5.89

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 144 - 426 2.4e-35 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 462
AciI CCGC 3 cut(s) 71, 117, 1062
AclWI GGATC 5 cut(s) 577, 804, 974, 1088, 1184
AcsI RAATTY 4 cut(s) 518, 610, 812, 1203
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 4 cut(s) 113, 572, 1014, 1267
AfiI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
AgeI ACCGGT 1 cut(s) 470
AjnI CCWGG 4 cut(s) 412, 506, 620, 1078
AluBI AGCT 5 cut(s) 164, 253, 288, 977, 1387
AluI AGCT 5 cut(s) 164, 253, 288, 977, 1387
Alw21I GWGCWC 2 cut(s) 145, 250
Alw26I GTCTC 3 cut(s) 30, 351, 763
AlwI GGATC 5 cut(s) 577, 804, 974, 1088, 1184
Ama87I CYCGRG 1 cut(s) 1322
AoxI GGCC 4 cut(s) 195, 365, 409, 664
ApaI GGGCCC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 1327
ApoI RAATTY 4 cut(s) 518, 610, 812, 1203
AsiGI ACCGGT 1 cut(s) 470
AspA2I CCTAGG 1 cut(s) 886
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 3 cut(s) 409, 410, 483
AsuC2I CCSGG 3 cut(s) 267, 283, 763
AsuHPI GGTGA 2 cut(s) 535, 855
AvaI CYCGRG 1 cut(s) 1322
AvaII GGWCC 1 cut(s) 483
AvrII CCTAGG 1 cut(s) 886
AxyI CCTNAGG 1 cut(s) 1356
BaeGI GKGCMC 2 cut(s) 264, 413
BanII GRGCYC 1 cut(s) 413
BbsI GAAGAC 2 cut(s) 750, 1037
Bbv12I GWGCWC 2 cut(s) 145, 250
BbvI GCAGC 1 cut(s) 1339
BccI CCATC 7 cut(s) 167, 233, 485, 625, 639, 698, 1169
BciT130I CCWGG 4 cut(s) 414, 508, 622, 1080
BclI TGATCA 1 cut(s) 435
BcnI CCSGG 3 cut(s) 267, 283, 763
BcoDI GTCTC 3 cut(s) 30, 351, 763
BfaI CTAG 3 cut(s) 300, 887, 1185
BfmI CTRYAG 2 cut(s) 978, 1129
BfoI RGCGCY 1 cut(s) 504
BisI GCNGC 3 cut(s) 72, 1063, 1328
BlnI CCTAGG 1 cut(s) 886
BlsI GCNGC 3 cut(s) 73, 1064, 1329
Bme1390I CCNGG 7 cut(s) 267, 283, 414, 508, 622, 763, 1080
Bme18I GGWCC 1 cut(s) 483
BmeT110I CYCGRG 1 cut(s) 1322
BmgT120I GGNCC 3 cut(s) 409, 410, 483
BmiI GGNNCC 2 cut(s) 411, 484
BmrFI CCNGG 7 cut(s) 267, 283, 414, 508, 622, 763, 1080
BmrI ACTGGG 2 cut(s) 59, 1162
BmsI GCATC 5 cut(s) 196, 242, 319, 841, 1218
BmuI ACTGGG 2 cut(s) 59, 1162
BpiI GAAGAC 2 cut(s) 750, 1037
BpuMI CCSGG 3 cut(s) 267, 283, 763
Bsa29I ATCGAT 1 cut(s) 1341
BsaAI YACGTR 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 351
BsaJI CCNNGG 5 cut(s) 265, 412, 506, 762, 886
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 4 cut(s) 598, 628, 743, 773
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 6 cut(s) 54, 226, 278, 445, 683, 1168
Bse21I CCTNAGG 1 cut(s) 1356
Bse3DI GCAATG 2 cut(s) 774, 1297
Bse8I GATNNNNATC 1 cut(s) 587
BseBI CCWGG 4 cut(s) 414, 508, 622, 1080
BseCI ATCGAT 1 cut(s) 1341
BseDI CCNNGG 5 cut(s) 265, 412, 506, 762, 886
BseJI GATNNNNATC 1 cut(s) 587
BseLI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
BseMI GCAATG 2 cut(s) 774, 1297
BseMII CTCAG 4 cut(s) 362, 1101, 1137, 1347
BseNI ACTGG 6 cut(s) 54, 226, 278, 445, 683, 1168
BseSI GKGCMC 2 cut(s) 264, 413
BseXI GCAGC 1 cut(s) 1339
BseYI CCCAGC 1 cut(s) 792
BshFI GGCC 4 cut(s) 197, 367, 411, 666
BshTI ACCGGT 1 cut(s) 470
BshVI ATCGAT 1 cut(s) 1341
BsiHKAI GWGCWC 2 cut(s) 145, 250
BsiHKCI CYCGRG 1 cut(s) 1322
BsiSI CCGG 5 cut(s) 130, 267, 282, 471, 762
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 8 cut(s) 70, 273, 325, 392, 492, 963, 1085, 1199
BsmAI GTCTC 3 cut(s) 30, 351, 763
BsmFI GGGAC 1 cut(s) 496
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 4 cut(s) 197, 367, 411, 666
Bso31I GGTCTC 1 cut(s) 351
BsoBI CYCGRG 1 cut(s) 1322
Bsp120I GGGCCC 1 cut(s) 409
Bsp1286I GDGCHC 4 cut(s) 145, 250, 264, 413
Bsp143I GATC 6 cut(s) 435, 582, 796, 966, 1093, 1189
BspACI CCGC 3 cut(s) 71, 117, 1062
BspANI GGCC 4 cut(s) 197, 367, 411, 666
BspCNI CTCAG 4 cut(s) 361, 1102, 1138, 1348
BspDI ATCGAT 1 cut(s) 1341
BspLI GGNNCC 2 cut(s) 411, 484
BspPI GGATC 5 cut(s) 577, 804, 974, 1088, 1184
BspTNI GGTCTC 1 cut(s) 351
BsrDI GCAATG 2 cut(s) 774, 1297
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 6 cut(s) 54, 226, 278, 445, 683, 1168
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 5 cut(s) 265, 412, 506, 762, 886
BssMI GATC 6 cut(s) 435, 582, 796, 966, 1093, 1189
BssNI GRCGYC 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 886
Bst2UI CCWGG 4 cut(s) 414, 508, 622, 1080
Bst4CI ACNGT 4 cut(s) 140, 749, 880, 1265
Bst6I CTCTTC 3 cut(s) 19, 375, 779
BstACI GRCGYC 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 1164, 1309
BstDEI CTNAG 5 cut(s) 221, 348, 1110, 1146, 1356
BstENI CCTNNNNNAGG 1 cut(s) 323
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 6 cut(s) 438, 585, 799, 969, 1096, 1192
BstMAI GTCTC 3 cut(s) 30, 351, 763
BstMBI GATC 6 cut(s) 435, 582, 796, 966, 1093, 1189
BstMWI GCNNNNNNNGC 5 cut(s) 259, 829, 881, 1287, 1327
BstNI CCWGG 4 cut(s) 414, 508, 622, 1080
BstSCI CCNGG 7 cut(s) 265, 281, 412, 506, 620, 761, 1078
BstSFI CTRYAG 2 cut(s) 978, 1129
BstSLI GKGCMC 2 cut(s) 264, 413
BstSNI TACGTA 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 1339
BstV2I GAAGAC 2 cut(s) 750, 1037
BstX2I RGATCY 1 cut(s) 582
BstXI CCANNNNNNTGG 1 cut(s) 1175
BstYI RGATCY 1 cut(s) 582
Bsu15I ATCGAT 1 cut(s) 1341
Bsu36I CCTNAGG 1 cut(s) 1356
BsuRI GGCC 4 cut(s) 197, 367, 411, 666
BsuTUI ATCGAT 1 cut(s) 1341
BtgZI GCGATG 1 cut(s) 186
BtsI GCAGTG 3 cut(s) 380, 786, 898
BtsIMutI CAGTG 6 cut(s) 145, 380, 438, 786, 898, 1233
Cac8I GCNNGC 2 cut(s) 1164, 1309
CfoI GCGC 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 3 cut(s) 409, 410, 483
ClaI ATCGAT 1 cut(s) 1341
Csp6I GTAC 4 cut(s) 112, 571, 1013, 1266
CspAI ACCGGT 1 cut(s) 470
CviAII CATG 1 cut(s) 29
CviQI GTAC 4 cut(s) 112, 571, 1013, 1266
DdeI CTNAG 5 cut(s) 221, 348, 1110, 1146, 1356
DpnI GATC 6 cut(s) 437, 584, 798, 968, 1095, 1191
DpnII GATC 6 cut(s) 435, 582, 796, 966, 1093, 1189
DraI TTTAAA 1 cut(s) 202
Eam1104I CTCTTC 3 cut(s) 19, 375, 779
EarI CTCTTC 3 cut(s) 19, 375, 779
EciI GGCGGA 1 cut(s) 106
Eco105I TACGTA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 886
Eco147I AGGCCT 2 cut(s) 197, 666
Eco24I GRGCYC 1 cut(s) 413
Eco31I GGTCTC 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 483
Eco81I CCTNAGG 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 1322
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO109I RGGNCCY 2 cut(s) 409, 410
EcoRI GAATTC 1 cut(s) 610
EcoRII CCWGG 4 cut(s) 412, 506, 620, 1078
EcoT14I CCWWGG 1 cut(s) 886
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 886
FaeI CATG 1 cut(s) 32
FaqI GGGAC 1 cut(s) 496
FatI CATG 1 cut(s) 28
FbaI TGATCA 1 cut(s) 435
Fnu4HI GCNGC 3 cut(s) 72, 1063, 1328
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 3 cut(s) 72, 1063, 1328
FspBI CTAG 3 cut(s) 300, 887, 1185
GlaI GCGC 1 cut(s) 502
GluI GCNGC 3 cut(s) 72, 1063, 1328
GsaI CCCAGC 1 cut(s) 796
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 4 cut(s) 197, 367, 411, 666
HapII CCGG 5 cut(s) 130, 267, 282, 471, 762
HhaI GCGC 1 cut(s) 503
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HinfI GANTC 8 cut(s) 5, 356, 719, 807, 909, 953, 1114, 1297
HpaII CCGG 5 cut(s) 130, 267, 282, 471, 762
HphI GGTGA 2 cut(s) 535, 855
Hpy166II GTNNAC 2 cut(s) 728, 1073
Hpy188I TCNGA 4 cut(s) 161, 238, 361, 435
Hpy188III TCNNGA 4 cut(s) 350, 523, 844, 1109
Hpy8I GTNNAC 2 cut(s) 728, 1073
Hpy99I CGWCG 2 cut(s) 461, 464
HpyAV CCTTC 3 cut(s) 396, 414, 520
HpyCH4III ACNGT 4 cut(s) 140, 749, 880, 1265
HpyCH4IV ACGT 3 cut(s) 110, 459, 1044
HpyCH4V TGCA 6 cut(s) 209, 233, 680, 774, 779, 1209
HpyF10VI GCNNNNNNNGC 5 cut(s) 259, 829, 881, 1287, 1327
HpyF3I CTNAG 5 cut(s) 221, 348, 1110, 1146, 1356
HpySE526I ACGT 3 cut(s) 110, 459, 1044
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 1 cut(s) 501
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 6 cut(s) 435, 582, 796, 966, 1093, 1189
LmnI GCTCC 4 cut(s) 161, 245, 285, 508
Lsp1109I GCAGC 1 cut(s) 1339
LweI GCATC 5 cut(s) 196, 242, 319, 841, 1218
MaeI CTAG 3 cut(s) 300, 887, 1185
MaeII ACGT 3 cut(s) 110, 459, 1044
MaeIII GTNAC 9 cut(s) 106, 269, 473, 541, 647, 749, 934, 1153, 1294
MalI GATC 6 cut(s) 437, 584, 798, 968, 1095, 1191
MboI GATC 6 cut(s) 435, 582, 796, 966, 1093, 1189
MboII GAAGA 7 cut(s) 6, 362, 365, 755, 796, 941, 1042
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 4 cut(s) 145, 250, 264, 413
MluCI AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
MlyI GAGTC 2 cut(s) 365, 1291
MmeI TCCRAC 1 cut(s) 1152
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 7 cut(s) 96, 123, 201, 425, 711, 1383, 1405
MslI CAYNNNNRTG 2 cut(s) 327, 779
MspA1I CMGCKG 1 cut(s) 977
MspI CCGG 5 cut(s) 130, 267, 282, 471, 762
MspR9I CCNGG 7 cut(s) 267, 283, 414, 508, 622, 763, 1080
Mva1269I GAATGC 1 cut(s) 825
MvaI CCWGG 4 cut(s) 414, 508, 622, 1080
MwoI GCNNNNNNNGC 5 cut(s) 259, 829, 881, 1287, 1327
NciI CCSGG 3 cut(s) 267, 283, 763
NdeII GATC 6 cut(s) 435, 582, 796, 966, 1093, 1189
NlaIII CATG 1 cut(s) 32
NlaIV GGNNCC 2 cut(s) 411, 484
NmuCI GTSAC 3 cut(s) 541, 934, 1294
NsiI ATGCAT 1 cut(s) 235
PceI AGGCCT 2 cut(s) 197, 666
PctI GAATGC 1 cut(s) 825
PfeI GAWTC 5 cut(s) 719, 807, 909, 953, 1114
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 3 cut(s) 73, 1064, 1329
PleI GAGTC 2 cut(s) 364, 1291
PpsI GAGTC 2 cut(s) 364, 1291
Ppu21I YACGTR 1 cut(s) 111
Psp6I CCWGG 4 cut(s) 412, 506, 620, 1078
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 4 cut(s) 412, 506, 620, 1078
PspN4I GGNNCC 2 cut(s) 411, 484
PspOMI GGGCCC 1 cut(s) 409
PspPI GGNCC 3 cut(s) 409, 410, 483
PsrI GAACNNNNNNTAC 2 cut(s) 436, 468
PsuI RGATCY 1 cut(s) 582
PvuII CAGCTG 1 cut(s) 977
RsaI GTAC 4 cut(s) 113, 572, 1014, 1267
RsaNI GTAC 4 cut(s) 112, 571, 1013, 1266
RseI CAYNNNNRTG 2 cut(s) 327, 779
SaqAI TTAA 7 cut(s) 96, 123, 201, 425, 711, 1383, 1405
SatI GCNGC 3 cut(s) 72, 1063, 1328
Sau3AI GATC 6 cut(s) 435, 582, 796, 966, 1093, 1189
Sau96I GGNCC 3 cut(s) 409, 410, 483
SchI GAGTC 2 cut(s) 365, 1291
ScrFI CCNGG 7 cut(s) 267, 283, 414, 508, 622, 763, 1080
SduI GDGCHC 4 cut(s) 145, 250, 264, 413
SfaNI GCATC 5 cut(s) 196, 242, 319, 841, 1218
SfcI CTRYAG 2 cut(s) 978, 1129
SinI GGWCC 1 cut(s) 483
SmiMI CAYNNNNRTG 2 cut(s) 327, 779
SnaBI TACGTA 1 cut(s) 111
Sse9I AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
SseBI AGGCCT 2 cut(s) 197, 666
SsiI CCGC 3 cut(s) 71, 117, 1062
SspI AATATT 1 cut(s) 94
SspMI CTAG 3 cut(s) 300, 887, 1185
StuI AGGCCT 2 cut(s) 197, 666
StyD4I CCNGG 7 cut(s) 265, 281, 412, 506, 620, 761, 1078
StyI CCWWGG 1 cut(s) 886
TaaI ACNGT 4 cut(s) 140, 749, 880, 1265
TaiI ACGT 3 cut(s) 113, 462, 1047
TaqI TCGA 2 cut(s) 736, 1341
TasI AATT 9 cut(s) 255, 465, 518, 610, 738, 812, 1179, 1203, 1250
TatI WGTACW 2 cut(s) 570, 1265
TauI GCSGC 2 cut(s) 74, 1065
TfiI GAWTC 5 cut(s) 719, 807, 909, 953, 1114
Tru1I TTAA 7 cut(s) 96, 123, 201, 425, 711, 1383, 1405
Tru9I TTAA 7 cut(s) 96, 123, 201, 425, 711, 1383, 1405
TscAI CASTG 6 cut(s) 145, 387, 445, 786, 898, 1240
TseFI GTSAC 3 cut(s) 541, 934, 1294
TseI GCWGC 1 cut(s) 1327
Tsp45I GTSAC 3 cut(s) 541, 934, 1294
TspDTI ATGAA 2 cut(s) 378, 1052
TspGWI ACGGA 1 cut(s) 1030
TspRI CASTG 6 cut(s) 145, 387, 445, 786, 898, 1240
VpaK11BI GGWCC 1 cut(s) 483
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 4 cut(s) 518, 610, 812, 1203
XmaJI CCTAGG 1 cut(s) 886
XspI CTAG 3 cut(s) 300, 887, 1185
ZraI GACGTC 1 cut(s) 460
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.