MD07G1279000.v1.1

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002711
Physical Location & Seq
Forward (+)
34347336 .. 34350072
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1279000.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGGAGGCCTCCTCAAAATTCCATGTTGCTTCTGCGATCATTGTTGCGCTGGGATTTTTCTTCCTAACAGCGGCAGAATGTCGAAAACCGGTTTCGGGCACGATCGAGTACTCGGCCATTAACTGTCGAAAACATAGTGCTCTTTTGACCGACTTTGGAGCCGTTGGCGACGGAAAAACTTCCAACACAAAGGCCTTCAAGGCTGCTGTTGGCCATCTAAGTCAGCTTGCATCCGACGGCGGAGCGCAGCTTATTGTGCCGCCGGGGAGGTGGCTCACCGGAAGCTTCAACCTCACTAGCCACTTTACCCTCTTCCTCCATAAGGATGCCGTGATTCTTGGAACTCAGGATGAGTCAGAGTGGCCTCATCTTCCAGTGCTGCCTTCATATGGGAGAGGCAGAGATGCCCCCGGTGGAAGGTTCAGCAGTCTCGTCTTCGGAACAAACCTCACCGACGTTGTAATTACCGGTAACAACGGCACAATCGACGGCCAAGGTGCTTCTTGGTGGGCAAAGTTCAAACAAGGTCACTTGACCCTTACCCGACCATACATGATCGAGATCATGTACTCTAATCAGATTCAAATTTCTAATCTCACTTTGGTCAACTCCCCTTCGTGGTTTGTCCATCCCATATACAGCAGCAATATAACAATCCAAGGGCTCACAATCCTTGCACCAGTTGATTCTCCCAACACCGATGGGATTGACCCAGATTCATGTTCACAAACAAGAATCGAAGACTGCTACATTGTCTCTGGAGATGACTGCATTGCAGTAAAGAGTGGATGGGACGAATACGGAATCAAAGTCGGAATCCCAACGGAGCACCTTGTGATCAGAAGGCTCACTTGCATTTCACCTGACAGTGCCACCATTGCCCTAGGCAGTGAAATGTCGGGTGGAATCCGCGACGTTAGAGCTGAGGACATCACAGCCATTGACACTCAATCGAGTGTGAGGATCAAAACTGCCGTGGGAAGAGGAGCTTATGTCAAAGACATTTATGTGAGAAGAATGACCTTGAAGACAATGAAGTATGTCTTTTGGATGACCGGCTCTTATGGATCGCACCCTGACCCCAGCTTTGATCCTAAAGCATTGCCTGAGATCACAAACATCAATTACAAGCAGGTTGTGGCAGAAAATGTAACTTATTCCGCAAGGCTGGACGGAGTCCCTAATGATCCTTTTAAGGGAATTTGCATTTCGAATGTGACCATCACACTAACTGAGAAGCCGAAAAAATTGCAATGGAACTGCACTAATGTTGAGGGAATCACAAGCAATGTGACCCCAAAGGCGTGCGATTTGTTGCCCGAGAAGAAAGAAGTGGTTGATTGTGCTTTCCCTGAGGATAGGTTGGCGATCGAGGATGTTGAGTTGGTGACTTGCTCTGCAAGCACCCCCTTCTTCCGAGGGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

476

Amino Acids

51.74

Weight (kDa)

5.96

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 144 - 425 3e-37 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1123
AccII CGCG 1 cut(s) 912
AciI CCGC 5 cut(s) 71, 240, 260, 910, 1161
AclWI GGATC 4 cut(s) 971, 1075, 1085, 1181
AcoI YGGCCR 3 cut(s) 114, 211, 490
AcsI RAATTY 3 cut(s) 17, 585, 1200
AdeI CACNNNGTG 1 cut(s) 835
AfaI GTAC 2 cut(s) 110, 569
AfiI CCNNNNNNNGG 7 cut(s) 70, 95, 322, 389, 417, 618, 1196
AgeI ACCGGT 2 cut(s) 88, 467
AgsI TTSAA 5 cut(s) 199, 289, 520, 584, 1027
AluBI AGCT 6 cut(s) 226, 250, 285, 923, 989, 1086
AluI AGCT 6 cut(s) 226, 250, 285, 923, 989, 1086
Alw21I GWGCWC 2 cut(s) 142, 831
Alw26I GTCTC 2 cut(s) 434, 760
AlwI GGATC 4 cut(s) 971, 1075, 1085, 1181
Ama87I CYCGRG 1 cut(s) 1319
AoxI GGCC 6 cut(s) 6, 114, 192, 211, 362, 490
ApeKI GCWGC 4 cut(s) 203, 247, 379, 642
ApoI RAATTY 3 cut(s) 17, 585, 1200
ArsI GACNNNNNNTTYG 2 cut(s) 1070, 1102
AsiGI ACCGGT 2 cut(s) 88, 467
Asp700I GAANNNNTTC 1 cut(s) 178
AspA2I CCTAGG 1 cut(s) 883
AspLEI GCGC 2 cut(s) 49, 247
AsuC2I CCSGG 2 cut(s) 264, 411
AsuHPI GGTGA 4 cut(s) 268, 442, 852, 1399
AsuII TTCGAA 1 cut(s) 1211
AvaI CYCGRG 1 cut(s) 1319
AvrII CCTAGG 1 cut(s) 883
AxyI CCTNAGG 1 cut(s) 1353
BaeGI GKGCMC 1 cut(s) 101
BalI TGGCCA 1 cut(s) 213
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 3 cut(s) 427, 747, 1034
Bbv12I GWGCWC 2 cut(s) 142, 831
BbvCI CCTCAGC 1 cut(s) 924
BbvI GCAGC 4 cut(s) 190, 259, 366, 654
BccI CCATC 5 cut(s) 222, 636, 695, 783, 1229
BceAI ACGGC 6 cut(s) 146, 253, 314, 493, 505, 959
BcgI CGANNNNNNTGC 2 cut(s) 1231, 1265
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 2 cut(s) 264, 411
BcoDI GTCTC 2 cut(s) 434, 760
BfaI CTAG 2 cut(s) 297, 884
BfuAI ACCTGC 1 cut(s) 1123
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 6 cut(s) 72, 204, 248, 260, 380, 643
BlnI CCTAGG 1 cut(s) 883
BlsI GCNGC 6 cut(s) 73, 205, 249, 261, 381, 644
BmcAI AGTACT 1 cut(s) 110
Bme1390I CCNGG 2 cut(s) 264, 411
BmeT110I CYCGRG 1 cut(s) 1319
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 2 cut(s) 264, 411
BmsI GCATC 3 cut(s) 239, 316, 394
BpiI GAAGAC 3 cut(s) 427, 747, 1034
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 780
Bpu10I CCTNAGC 1 cut(s) 924
Bpu14I TTCGAA 1 cut(s) 1211
BpuMI CCSGG 2 cut(s) 264, 411
BsaBI GATNNNNATC 1 cut(s) 560
BsaJI CCNNGG 7 cut(s) 263, 409, 493, 658, 883, 975, 1417
BsaWI WCCGGW 3 cut(s) 88, 278, 467
BsaXI ACNNNNNCTCC 2 cut(s) 978, 1008
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 95, 322, 389, 417, 618, 1196
Bse118I RCCGGY 3 cut(s) 88, 467, 1055
Bse1I ACTGG 2 cut(s) 374, 680
Bse21I CCTNAGG 1 cut(s) 1353
Bse3DI GCAATG 5 cut(s) 771, 876, 1100, 1259, 1294
Bse8I GATNNNNATC 1 cut(s) 560
BseDI CCNNGG 7 cut(s) 263, 409, 493, 658, 883, 975, 1417
BseGI GGATG 7 cut(s) 230, 331, 355, 628, 794, 1056, 1381
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 7 cut(s) 70, 95, 322, 389, 417, 618, 1196
BseMI GCAATG 5 cut(s) 771, 876, 1100, 1259, 1294
BseMII CTCAG 5 cut(s) 359, 915, 1098, 1224, 1344
BseNI ACTGG 2 cut(s) 374, 680
BseRI GAGGAG 1 cut(s) 999
BseSI GKGCMC 1 cut(s) 101
BseXI GCAGC 4 cut(s) 190, 259, 366, 654
BseYI CCCAGC 2 cut(s) 49, 1082
BsgI GTGCAG 1 cut(s) 1246
Bsh1236I CGCG 1 cut(s) 912
Bsh1285I CGRYCG 2 cut(s) 105, 1371
BshFI GGCC 6 cut(s) 8, 116, 194, 213, 364, 492
BshTI ACCGGT 2 cut(s) 88, 467
BsiEI CGRYCG 2 cut(s) 105, 1371
BsiHKAI GWGCWC 2 cut(s) 142, 831
BsiHKCI CYCGRG 1 cut(s) 1319
BsiSI CCGG 6 cut(s) 89, 263, 279, 411, 468, 1056
BslFI GGGAC 2 cut(s) 806, 1163
BslI CCNNNNNNNGG 7 cut(s) 70, 95, 322, 389, 417, 618, 1196
BsmAI GTCTC 2 cut(s) 434, 760
BsmFI GGGAC 2 cut(s) 806, 1163
BsnI GGCC 6 cut(s) 8, 116, 194, 213, 364, 492
BsoBI CYCGRG 1 cut(s) 1319
Bsp119I TTCGAA 1 cut(s) 1211
Bsp1286I GDGCHC 4 cut(s) 101, 142, 666, 831
BspACI CCGC 5 cut(s) 71, 240, 260, 910, 1161
BspANI GGCC 6 cut(s) 8, 116, 194, 213, 364, 492
BspCNI CTCAG 5 cut(s) 358, 916, 1099, 1225, 1345
BspFNI CGCG 1 cut(s) 912
BspLI GGNNCC 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 1123
BspPI GGATC 4 cut(s) 971, 1075, 1085, 1181
BspT104I TTCGAA 1 cut(s) 1211
BsrDI GCAATG 5 cut(s) 771, 876, 1100, 1259, 1294
BsrFI RCCGGY 3 cut(s) 88, 467, 1055
BsrI ACTGG 2 cut(s) 374, 680
BssAI RCCGGY 3 cut(s) 88, 467, 1055
BssECI CCNNGG 7 cut(s) 263, 409, 493, 658, 883, 975, 1417
BssT1I CCWWGG 3 cut(s) 493, 658, 883
Bst4CI ACNGT 2 cut(s) 125, 869
Bst6I CTCTTC 2 cut(s) 317, 976
BstBI TTCGAA 1 cut(s) 1211
BstC8I GCNNGC 3 cut(s) 228, 1306, 1402
BstDEI CTNAG 6 cut(s) 218, 345, 924, 1107, 1233, 1353
BstDSI CCRYGG 1 cut(s) 975
BstENI CCTNNNNNAGG 1 cut(s) 320
BstF5I GGATG 7 cut(s) 230, 331, 355, 628, 794, 1056, 1381
BstFNI CGCG 1 cut(s) 912
BstHHI GCGC 2 cut(s) 49, 247
BstMAI GTCTC 2 cut(s) 434, 760
BstMCI CGRYCG 2 cut(s) 105, 1371
BstMWI GCNNNNNNNGC 4 cut(s) 200, 256, 878, 1401
BstSCI CCNGG 2 cut(s) 262, 409
BstSLI GKGCMC 1 cut(s) 101
BstUI CGCG 1 cut(s) 912
BstV1I GCAGC 4 cut(s) 190, 259, 366, 654
BstV2I GAAGAC 3 cut(s) 427, 747, 1034
Bsu36I CCTNAGG 1 cut(s) 1353
BsuRI GGCC 6 cut(s) 8, 116, 194, 213, 364, 492
BtgI CCRYGG 1 cut(s) 975
BtsCI GGATG 7 cut(s) 230, 331, 355, 628, 794, 1056, 1381
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 3 cut(s) 381, 874, 895
BveI ACCTGC 1 cut(s) 1123
Cac8I GCNNGC 3 cut(s) 228, 1306, 1402
CfoI GCGC 2 cut(s) 49, 247
Cfr10I RCCGGY 3 cut(s) 88, 467, 1055
Csp6I GTAC 2 cut(s) 109, 568
CspAI ACCGGT 2 cut(s) 88, 467
CviAII CATG 4 cut(s) 23, 553, 565, 720
CviQI GTAC 2 cut(s) 109, 568
DdeI CTNAG 6 cut(s) 218, 345, 924, 1107, 1233, 1353
DraIII CACNNNGTG 1 cut(s) 835
EaeI YGGCCR 3 cut(s) 114, 211, 490
Eam1104I CTCTTC 2 cut(s) 317, 976
EarI CTCTTC 2 cut(s) 317, 976
EciI GGCGGA 1 cut(s) 255
Eco130I CCWWGG 3 cut(s) 493, 658, 883
Eco147I AGGCCT 2 cut(s) 8, 194
Eco24I GRGCYC 1 cut(s) 666
Eco81I CCTNAGG 1 cut(s) 1353
Eco88I CYCGRG 1 cut(s) 1319
EcoNI CCTNNNNNAGG 1 cut(s) 320
EcoT14I CCWWGG 3 cut(s) 493, 658, 883
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 3 cut(s) 493, 658, 883
FaeI CATG 4 cut(s) 26, 556, 568, 723
FalI AAGNNNNNCTT 6 cut(s) 835, 867, 973, 1005, 1028, 1060
FaqI GGGAC 2 cut(s) 806, 1163
FatI CATG 4 cut(s) 22, 552, 564, 719
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 837
Fnu4HI GCNGC 6 cut(s) 72, 204, 248, 260, 380, 643
FokI GGATG 7 cut(s) 217, 338, 362, 615, 801, 1063, 1388
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 6 cut(s) 72, 204, 248, 260, 380, 643
FspBI CTAG 2 cut(s) 297, 884
GlaI GCGC 2 cut(s) 48, 246
GluI GCNGC 6 cut(s) 72, 204, 248, 260, 380, 643
GsaI CCCAGC 2 cut(s) 53, 1086
GsuI CTGGAG 1 cut(s) 780
HaeIII GGCC 6 cut(s) 8, 116, 194, 213, 364, 492
HapII CCGG 6 cut(s) 89, 263, 279, 411, 468, 1056
HhaI GCGC 2 cut(s) 49, 247
Hin1II CATG 4 cut(s) 26, 556, 568, 723
Hin6I GCGC 2 cut(s) 47, 245
HinP1I GCGC 2 cut(s) 47, 245
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HindIII AAGCTT 1 cut(s) 283
HpaII CCGG 6 cut(s) 89, 263, 279, 411, 468, 1056
HphI GGTGA 4 cut(s) 268, 442, 852, 1399
Hpy166II GTNNAC 2 cut(s) 607, 725
Hpy188I TCNGA 7 cut(s) 235, 358, 440, 579, 815, 842, 1418
Hpy188III TCNNGA 3 cut(s) 347, 559, 759
Hpy8I GTNNAC 2 cut(s) 607, 725
Hpy99I CGWCG 5 cut(s) 173, 239, 458, 491, 917
HpyAV CCTTC 6 cut(s) 205, 393, 411, 624, 837, 1420
HpyCH4III ACNGT 2 cut(s) 125, 869
HpyCH4IV ACGT 2 cut(s) 456, 915
HpyCH4V TGCA 9 cut(s) 230, 677, 771, 776, 855, 1206, 1252, 1263, 1400
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 256, 878, 1401
HpyF3I CTNAG 6 cut(s) 218, 345, 924, 1107, 1233, 1353
HpySE526I ACGT 2 cut(s) 456, 915
Hsp92II CATG 4 cut(s) 26, 556, 568, 723
HspAI GCGC 2 cut(s) 47, 245
Ksp22I TGATCA 1 cut(s) 837
LmnI GCTCC 4 cut(s) 158, 242, 826, 986
Lsp1109I GCAGC 4 cut(s) 190, 259, 366, 654
LweI GCATC 3 cut(s) 239, 316, 394
MaeI CTAG 2 cut(s) 297, 884
MaeII ACGT 2 cut(s) 456, 915
MaeIII GTNAC 6 cut(s) 470, 527, 1150, 1216, 1291, 1387
MhlI GDGCHC 4 cut(s) 101, 142, 666, 831
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 6 cut(s) 17, 462, 585, 1123, 1200, 1247
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 2 cut(s) 362, 1185
MmeI TCCRAC 3 cut(s) 207, 258, 793
Mox20I TGGCCA 1 cut(s) 213
MroXI GAANNNNTTC 1 cut(s) 178
MscI TGGCCA 1 cut(s) 213
MseI TTAA 2 cut(s) 120, 1194
MslI CAYNNNNRTG 2 cut(s) 324, 1007
Msp20I TGGCCA 1 cut(s) 213
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 6 cut(s) 89, 263, 279, 411, 468, 1056
MspR9I CCNGG 2 cut(s) 264, 411
MvnI CGCG 1 cut(s) 912
MwoI GCNNNNNNNGC 4 cut(s) 200, 256, 878, 1401
NciI CCSGG 2 cut(s) 264, 411
NdeI CATATG 1 cut(s) 388
NlaIII CATG 4 cut(s) 26, 556, 568, 723
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 4 cut(s) 527, 1216, 1291, 1387
NspV TTCGAA 1 cut(s) 1211
PceI AGGCCT 2 cut(s) 8, 194
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 9 cut(s) 334, 580, 686, 716, 735, 804, 816, 906, 1278
PflFI GACNNNGTC 1 cut(s) 1175
PinAI ACCGGT 2 cut(s) 88, 467
PkrI GCNGC 6 cut(s) 73, 205, 249, 261, 381, 644
Ple19I CGATCG 2 cut(s) 105, 1371
PleI GAGTC 2 cut(s) 361, 1184
PpsI GAGTC 2 cut(s) 361, 1184
PspFI CCCAGC 2 cut(s) 49, 1082
PspN4I GGNNCC 1 cut(s) 160
PsyI GACNNNGTC 1 cut(s) 1175
PvuI CGATCG 2 cut(s) 105, 1371
RsaI GTAC 2 cut(s) 110, 569
RsaNI GTAC 2 cut(s) 109, 568
RseI CAYNNNNRTG 2 cut(s) 324, 1007
SaqAI TTAA 2 cut(s) 120, 1194
SatI GCNGC 6 cut(s) 72, 204, 248, 260, 380, 643
ScaI AGTACT 1 cut(s) 110
SchI GAGTC 2 cut(s) 362, 1185
ScrFI CCNGG 2 cut(s) 264, 411
SduI GDGCHC 4 cut(s) 101, 142, 666, 831
SfaNI GCATC 3 cut(s) 239, 316, 394
SfuI TTCGAA 1 cut(s) 1211
SmiMI CAYNNNNRTG 2 cut(s) 324, 1007
Sse9I AATT 6 cut(s) 17, 462, 585, 1123, 1200, 1247
SseBI AGGCCT 2 cut(s) 8, 194
SsiI CCGC 5 cut(s) 71, 240, 260, 910, 1161
SspMI CTAG 2 cut(s) 297, 884
StuI AGGCCT 2 cut(s) 8, 194
StyD4I CCNGG 2 cut(s) 262, 409
StyI CCWWGG 3 cut(s) 493, 658, 883
TaaI ACNGT 2 cut(s) 125, 869
TaiI ACGT 2 cut(s) 459, 918
TaqI TCGA 9 cut(s) 82, 105, 127, 486, 558, 738, 953, 1211, 1371
TaqII GACCGA 1 cut(s) 164
TasI AATT 6 cut(s) 17, 462, 585, 1123, 1200, 1247
TatI WGTACW 2 cut(s) 108, 567
TauI GCSGC 2 cut(s) 74, 262
TfiI GAWTC 9 cut(s) 334, 580, 686, 716, 735, 804, 816, 906, 1278
Tru1I TTAA 2 cut(s) 120, 1194
Tru9I TTAA 2 cut(s) 120, 1194
TscAI CASTG 3 cut(s) 381, 874, 895
TseFI GTSAC 4 cut(s) 527, 1216, 1291, 1387
TseI GCWGC 4 cut(s) 203, 247, 379, 642
Tsp45I GTSAC 4 cut(s) 527, 1216, 1291, 1387
TspDTI ATGAA 3 cut(s) 375, 708, 1049
TspGWI ACGGA 4 cut(s) 186, 816, 839, 1188
TspRI CASTG 3 cut(s) 381, 874, 895
Tth111I GACNNNGTC 1 cut(s) 1175
XagI CCTNNNNNAGG 1 cut(s) 320
XapI RAATTY 3 cut(s) 17, 585, 1200
XmaJI CCTAGG 1 cut(s) 883
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 2 cut(s) 297, 884
ZrmI AGTACT 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.