MD14G1086700.v1.1

Glutaminyl-peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007377
Physical Location & Seq
Reverse (-)
10725419 .. 10726519
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1086700.v1.1.491

Sequence Viewer

Length: 285 bp
ATGGATGGTGATTTTTTGTGTTCGTCTGAACTAATGGCCGCTTTTCTCATGCTCGGCGTTCTAATTCTCCTCGGCATTTCGTCGAACATGTGGAGCACCGTCATCTACGCCGACGTCTCCCCTGGAATTTACTCGATCGAGGTCGTCAATGAGTTCCCTCACGATCCCGACGCCTTCACCCAGGGGCTACTTGATGCTGGAAATGATAGCTTGTTCGAGTCGACTGGGCTTTACGGGAAGTCATCAGTGCGCAAGGTTGCGATTCGAATTGAGATATGTAGGTGA

Protein Analysis

95

Amino Acids

10.22

Weight (kDa)

4.24

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_cyclase_2 PF05096 39 - 88 3.2e-14 Glutamine cyclotransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
Acc16I TGCGCA 1 cut(s) 251
AccI GTMKAC 1 cut(s) 221
AciI CCGC 1 cut(s) 39
AclWI GGATC 1 cut(s) 158
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 126
AcyI GRCGYC 2 cut(s) 114, 171
AflIII ACRYGT 1 cut(s) 87
AjnI CCWGG 2 cut(s) 121, 180
AluBI AGCT 1 cut(s) 210
AluI AGCT 1 cut(s) 210
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 158
AoxI GGCC 1 cut(s) 36
ApoI RAATTY 1 cut(s) 126
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 2 cut(s) 20, 169
AsuII TTCGAA 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 98
BciT130I CCWGG 2 cut(s) 123, 182
BcoDI GTCTC 1 cut(s) 121
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 123, 182
BmrFI CCNGG 2 cut(s) 123, 182
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 184
BmuI ACTGGG 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 265
BsaHI GRCGYC 2 cut(s) 114, 171
BsaJI CCNNGG 4 cut(s) 70, 121, 180, 181
Bse1I ACTGG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 123, 182
BseDI CCNNGG 4 cut(s) 70, 121, 180, 181
BseGI GGATG 1 cut(s) 10
BseNI ACTGG 1 cut(s) 229
BseRI GAGGAG 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 38
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 98
BsmAI GTCTC 1 cut(s) 121
BsmBI CGTCTC 1 cut(s) 121
BsnI GGCC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 265
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 2 cut(s) 135, 163
BspACI CCGC 1 cut(s) 39
BspANI GGCC 1 cut(s) 38
BspPI GGATC 1 cut(s) 158
BspT104I TTCGAA 1 cut(s) 265
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 4 cut(s) 70, 121, 180, 181
BssMI GATC 2 cut(s) 135, 163
BssNI GRCGYC 2 cut(s) 114, 171
Bst2UI CCWGG 2 cut(s) 123, 182
Bst4CI ACNGT 1 cut(s) 100
BstACI GRCGYC 2 cut(s) 114, 171
BstBI TTCGAA 1 cut(s) 265
BstF5I GGATG 1 cut(s) 10
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 2 cut(s) 138, 166
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 2 cut(s) 135, 163
BstMCI CGRYCG 1 cut(s) 138
BstNI CCWGG 2 cut(s) 123, 182
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 2 cut(s) 121, 180
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 252
CfoI GCGC 1 cut(s) 252
CseI GACGC 1 cut(s) 179
CviAII CATG 2 cut(s) 49, 88
CviJI RGCY 4 cut(s) 38, 187, 210, 229
CviKI_1 RGCY 4 cut(s) 38, 187, 210, 229
DpnI GATC 2 cut(s) 137, 165
DpnII GATC 2 cut(s) 135, 163
EaeI YGGCCR 1 cut(s) 36
EcoRII CCWGG 2 cut(s) 121, 180
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 2 cut(s) 52, 91
FaiI YATR 3 cut(s) 50, 89, 277
FatI CATG 2 cut(s) 48, 87
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 39
FspI TGCGCA 1 cut(s) 251
GlaI GCGC 1 cut(s) 251
GluI GCNGC 1 cut(s) 39
HaeIII GGCC 1 cut(s) 38
HgaI GACGC 1 cut(s) 179
HhaI GCGC 1 cut(s) 252
Hin1I GRCGYC 2 cut(s) 114, 171
Hin1II CATG 2 cut(s) 52, 91
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 2 cut(s) 218, 262
HphI GGTGA 2 cut(s) 20, 169
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 2 cut(s) 161, 167
Hpy8I GTNNAC 1 cut(s) 222
Hpy99I CGWCG 3 cut(s) 85, 116, 173
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4IV ACGT 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 114
Hsp92I GRCGYC 2 cut(s) 114, 171
Hsp92II CATG 2 cut(s) 52, 91
HspAI GCGC 1 cut(s) 250
Kzo9I GATC 2 cut(s) 135, 163
LmnI GCTCC 1 cut(s) 93
LpnPI CCDG 6 cut(s) 108, 135, 167, 183, 194, 210
LweI GCATC 1 cut(s) 184
MaeII ACGT 1 cut(s) 114
MalI GATC 2 cut(s) 137, 165
MboI GATC 2 cut(s) 135, 163
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 3 cut(s) 63, 126, 267
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 3 cut(s) 80, 133, 168
MspR9I CCNGG 2 cut(s) 123, 182
MvaI CCWGG 2 cut(s) 123, 182
NdeII GATC 2 cut(s) 135, 163
NlaIII CATG 2 cut(s) 52, 91
NmeAIII GCCGAG 2 cut(s) 33, 51
NsbI TGCGCA 1 cut(s) 251
NspI RCATGY 1 cut(s) 91
NspV TTCGAA 1 cut(s) 265
PasI CCCWGGG 1 cut(s) 181
PciI ACATGT 1 cut(s) 87
PfeI GAWTC 1 cut(s) 262
PkrI GCNGC 1 cut(s) 40
Ple19I CGATCG 1 cut(s) 138
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PscI ACATGT 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 121, 180
PspGI CCWGG 2 cut(s) 121, 180
PvuI CGATCG 1 cut(s) 138
SalI GTCGAC 1 cut(s) 220
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 2 cut(s) 135, 163
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 123, 182
SduI GDGCHC 1 cut(s) 98
SetI ASST 5 cut(s) 117, 144, 212, 258, 284
SfaNI GCATC 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 265
Sse9I AATT 3 cut(s) 63, 126, 267
SsiI CCGC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 121, 180
TaaI ACNGT 1 cut(s) 100
TaiI ACGT 1 cut(s) 117
TaqI TCGA 6 cut(s) 83, 134, 138, 216, 221, 265
TasI AATT 3 cut(s) 63, 126, 267
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 262
TscAI CASTG 1 cut(s) 252
TspRI CASTG 1 cut(s) 252
XapI RAATTY 1 cut(s) 126
XceI RCATGY 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 221
ZraI GACGTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.