Rw1G037620

Glutaminyl-peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007377
Physical Location & Seq
Reverse (-)
65276704 .. 65279353
2650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G037620.1

Sequence Viewer

Length: 870 bp
ATGGCCACTGGACCGCTTCGCAAGAGGCTCAATAAGCGATCCTCAGACCCCAAACCCCGCACTGCGATGGCAACCCGGCCTATTGGTTACAGCCCTGTGTTTACTGGAATTTATTCGATTGAGGTCGTCAATGAGTTCCCTCATGATCCCAATGCCTTCACCCAGGGGCTTCTTTATGCTGGAAATGGGAGCTTATTTGAGTCCACTGGGCTTCGTGGCCGGTCATCAGTGCGTAAGGTGGATATTCAAACAGGGAAGGTTGAGGTTCTTCAGGAGATGGATTCTAGATATTTTGGGGAGGGATTAACTCTTCTCGGTGAATGGCTATTCCAAGTAACTTGGTTGACGAAAATTGGTTTCAAATATGACCGAAATGATTTAAGCAAATCTGAAAAGTTCACTCATCAAATGCAAGATGGTTGGGGACTGGCTACTGATGGAAAAGTGTTATTTGGAAGTGATGGCTCATCCGTGTTGTACAAGATTGACCCTCAAACATTGAAAGTTATAGACAAACACGTAGTCAAATACAAAGATCATGAAGTGCACAACCTCAATGAGTTGGAGTTTATAAAAGGCGAAGTTTGGGCAAATGTTTATCAGACAGACTGCATTGCTAGAATCTCACAGGATGATGGTAGGGTGCTTGGATGGATTCTCCTTCCAAATTTAAGGGAGGGTTTACTAGCTGCTGGAAATAATGCTATCGATGTGCTAAACGGCATTGCATGGGATAGCGACACCAACCGTATTTTTGTGACTGGAAAACTATGGCCGAAGCTTTATGAGATCAAGCTGCATCCAATAAAGAAACAGTTCAAGGATGGAGCCATTGAGCAGCTATGCCTACGGCAGCCATTCTTTCTTTGA

Protein Analysis

289

Amino Acids

32.72

Weight (kDa)

8.67

Isoelectric Point (pI)

28.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_cyclase_2 PF05096 37 - 266 2.4e-91 Glutamine cyclotransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AciI CCGC 2 cut(s) 14, 58
AclWI GGATC 2 cut(s) 33, 140
AcoI YGGCCR 3 cut(s) 3, 217, 773
AcsI RAATTY 2 cut(s) 108, 667
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 1 cut(s) 479
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 4 cut(s) 248, 361, 502, 820
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 5 cut(s) 192, 689, 781, 796, 841
AluI AGCT 5 cut(s) 192, 689, 781, 796, 841
Alw21I GWGCWC 1 cut(s) 549
Alw44I GTGCAC 1 cut(s) 545
AlwI GGATC 2 cut(s) 33, 140
AoxI GGCC 4 cut(s) 3, 77, 217, 773
ApaLI GTGCAC 1 cut(s) 545
ApeKI GCWGC 4 cut(s) 689, 796, 838, 853
ApoI RAATTY 2 cut(s) 108, 667
ArsI GACNNNNNNTTYG 2 cut(s) 507, 539
Asp700I GAANNNNTTC 2 cut(s) 112, 815
AspS9I GGNCC 1 cut(s) 11
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 151, 329
AvaII GGWCC 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 549
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 676, 783, 850, 865
BccI CCATC 8 cut(s) 61, 271, 410, 431, 455, 629, 645, 818
BceAI ACGGC 2 cut(s) 736, 866
BciT130I CCWGG 1 cut(s) 164
BcnI CCSGG 1 cut(s) 76
BfaI CTAG 3 cut(s) 285, 618, 686
BisI GCNGC 4 cut(s) 690, 797, 839, 854
BlsI GCNGC 4 cut(s) 691, 798, 840, 855
Bme1390I CCNGG 2 cut(s) 76, 164
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 1 cut(s) 829
BmrFI CCNGG 2 cut(s) 76, 164
BmrI ACTGGG 1 cut(s) 216
BmsI GCATC 1 cut(s) 808
BmuI ACTGGG 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 76
Bsa29I ATCGAT 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 520
BsaJI CCNNGG 2 cut(s) 162, 163
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 5 cut(s) 13, 109, 211, 432, 766
Bse3DI GCAATG 2 cut(s) 612, 723
BseBI CCWGG 1 cut(s) 164
BseCI ATCGAT 1 cut(s) 708
BseDI CCNNGG 2 cut(s) 162, 163
BseGI GGATG 5 cut(s) 467, 637, 656, 799, 829
BseMI GCAATG 2 cut(s) 612, 723
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 5 cut(s) 13, 109, 211, 432, 766
BseSI GKGCMC 1 cut(s) 549
BseXI GCAGC 4 cut(s) 676, 783, 850, 865
BshFI GGCC 4 cut(s) 5, 79, 219, 775
BshVI ATCGAT 1 cut(s) 708
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 2 cut(s) 76, 220
BslFI GGGAC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 4 cut(s) 5, 79, 219, 775
Bsp1286I GDGCHC 1 cut(s) 549
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 4 cut(s) 38, 145, 535, 789
BspACI CCGC 2 cut(s) 14, 58
BspANI GGCC 4 cut(s) 5, 79, 219, 775
BspCNI CTCAG 1 cut(s) 56
BspDI ATCGAT 1 cut(s) 708
BspHI TCATGA 2 cut(s) 142, 538
BspLI GGNNCC 1 cut(s) 829
BspPI GGATC 2 cut(s) 33, 140
BsrDI GCAATG 2 cut(s) 612, 723
BsrFI RCCGGY 1 cut(s) 219
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 5 cut(s) 13, 109, 211, 432, 766
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 2 cut(s) 162, 163
BssMI GATC 4 cut(s) 38, 145, 535, 789
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 749, 816
Bst6I CTCTTC 1 cut(s) 315
BstAUI TGTACA 1 cut(s) 477
BstBAI YACGTR 1 cut(s) 520
BstDEI CTNAG 1 cut(s) 43
BstF5I GGATG 5 cut(s) 467, 637, 656, 799, 829
BstKTI GATC 4 cut(s) 41, 148, 538, 792
BstMBI GATC 4 cut(s) 38, 145, 535, 789
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNI CCWGG 1 cut(s) 164
BstSCI CCNGG 2 cut(s) 74, 162
BstSLI GKGCMC 1 cut(s) 549
BstV1I GCAGC 4 cut(s) 676, 783, 850, 865
Bsu15I ATCGAT 1 cut(s) 708
BsuRI GGCC 4 cut(s) 5, 79, 219, 775
BsuTUI ATCGAT 1 cut(s) 708
BtgZI GCGATG 1 cut(s) 80
BtsCI GGATG 5 cut(s) 467, 637, 656, 799, 829
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 4 cut(s) 6, 60, 204, 234
CciI TCATGA 2 cut(s) 142, 538
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 11
ClaI ATCGAT 1 cut(s) 708
Csp6I GTAC 1 cut(s) 478
CviAII CATG 3 cut(s) 143, 539, 729
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 1 cut(s) 43
DpnI GATC 4 cut(s) 40, 147, 537, 791
DpnII GATC 4 cut(s) 38, 145, 535, 789
EaeI YGGCCR 3 cut(s) 3, 217, 773
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 162
FaeI CATG 3 cut(s) 146, 542, 732
FaqI GGGAC 1 cut(s) 438
FatI CATG 3 cut(s) 142, 538, 728
FauI CCCGC 1 cut(s) 65
Fnu4HI GCNGC 4 cut(s) 690, 797, 839, 854
FokI GGATG 5 cut(s) 454, 644, 663, 786, 836
Fsp4HI GCNGC 4 cut(s) 690, 797, 839, 854
FspBI CTAG 3 cut(s) 285, 618, 686
GluI GCNGC 4 cut(s) 690, 797, 839, 854
HaeIII GGCC 4 cut(s) 5, 79, 219, 775
HapII CCGG 2 cut(s) 76, 220
Hin1II CATG 3 cut(s) 146, 542, 732
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 4 cut(s) 200, 281, 621, 655
HpaII CCGG 2 cut(s) 76, 220
HphI GGTGA 2 cut(s) 151, 329
Hpy166II GTNNAC 6 cut(s) 102, 204, 345, 399, 547, 683
Hpy188I TCNGA 3 cut(s) 46, 391, 603
Hpy188III TCNNGA 4 cut(s) 143, 272, 285, 539
Hpy8I GTNNAC 6 cut(s) 102, 204, 345, 399, 547, 683
HpyAV CCTTC 3 cut(s) 166, 250, 671
HpyCH4III ACNGT 2 cut(s) 749, 816
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 5 cut(s) 412, 547, 612, 728, 799
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 1 cut(s) 519
Hsp92II CATG 3 cut(s) 146, 542, 732
Kzo9I GATC 4 cut(s) 38, 145, 535, 789
LmnI GCTCC 2 cut(s) 189, 827
Lsp1109I GCAGC 4 cut(s) 676, 783, 850, 865
LweI GCATC 1 cut(s) 808
MaeI CTAG 3 cut(s) 285, 618, 686
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 3 cut(s) 86, 334, 757
MalI GATC 4 cut(s) 40, 147, 537, 791
MboI GATC 4 cut(s) 38, 145, 535, 789
MboII GAAGA 2 cut(s) 260, 302
MhlI GDGCHC 1 cut(s) 549
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 108, 351, 667
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 9 cut(s) 18, 52, 115, 150, 256, 292, 501, 563, 670
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 112, 815
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 305, 380, 671
MslI CAYNNNNRTG 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 76, 220
MspR9I CCNGG 2 cut(s) 76, 164
MvaI CCWGG 1 cut(s) 164
MwoI GCNNNNNNNGC 1 cut(s) 34
NciI CCSGG 1 cut(s) 76
NdeII GATC 4 cut(s) 38, 145, 535, 789
NlaIII CATG 3 cut(s) 146, 542, 732
NlaIV GGNNCC 1 cut(s) 829
NmuCI GTSAC 1 cut(s) 757
PagI TCATGA 2 cut(s) 142, 538
PasI CCCWGGG 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 112, 815
PfeI GAWTC 3 cut(s) 281, 621, 655
PkrI GCNGC 4 cut(s) 691, 798, 840, 855
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
Ppu21I YACGTR 1 cut(s) 520
PsiI TTATAA 1 cut(s) 572
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 829
PspPI GGNCC 1 cut(s) 11
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 3 cut(s) 305, 380, 671
SatI GCNGC 4 cut(s) 690, 797, 839, 854
Sau3AI GATC 4 cut(s) 38, 145, 535, 789
Sau96I GGNCC 1 cut(s) 11
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 2 cut(s) 76, 164
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 1 cut(s) 808
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 65
Sse9I AATT 3 cut(s) 108, 351, 667
SsiI CCGC 2 cut(s) 14, 58
SspMI CTAG 3 cut(s) 285, 618, 686
StyD4I CCNGG 2 cut(s) 74, 162
TaaI ACNGT 2 cut(s) 749, 816
TaiI ACGT 1 cut(s) 522
TaqI TCGA 2 cut(s) 116, 708
TaqII GACCGA 1 cut(s) 384
TasI AATT 3 cut(s) 108, 351, 667
TatI WGTACW 1 cut(s) 477
TfiI GAWTC 3 cut(s) 281, 621, 655
Tru1I TTAA 3 cut(s) 305, 380, 671
Tru9I TTAA 3 cut(s) 305, 380, 671
TscAI CASTG 4 cut(s) 13, 67, 211, 234
TseFI GTSAC 1 cut(s) 757
TseI GCWGC 4 cut(s) 689, 796, 838, 853
Tsp45I GTSAC 1 cut(s) 757
TspDTI ATGAA 1 cut(s) 555
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 4 cut(s) 13, 67, 211, 234
VneI GTGCAC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 2 cut(s) 108, 667
XbaI TCTAGA 1 cut(s) 284
XmnI GAANNNNTTC 2 cut(s) 112, 815
XspI CTAG 3 cut(s) 285, 618, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.