FvH4_7g30250

Glutaminyl-peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007377
Physical Location & Seq
Reverse (-)
22087261 .. 22090522
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30250.t5

Sequence Viewer

Length: 975 bp
ATGCCCACCCGATCACTTCGCCACAGGCCCAATAAGCGACCCTCAAACCCCAAACCCCACACTCCCATGGCTCCCGGCCGTCCTACTTCCTCCAAGTCCTACAAAACCACCTCTCTCATTCTCCTCATGTTTCTCATCCTCGCCGCTCTCGCTCTCTTCAGCGTCTCCTCCAACACGTGGAGCTCACCCGGGAACGACGCCGCGTTTAACGCAGTTTACTCCATTCAGGTCGTTAATCAGTTCCCTCATGACCCCAATGCCTTCACTCAGGGGCTTCTTTATGCCGGAAATGGGAGCCTGTTTGAGTCCACTGGGCTTTATGGCAGGTCAACAGTGCGCAAGGTGGATATTCAAACAGGCAAGGTTGAGGTTCTTCAGGAGATGGATTCTAAATATTTTGGGGAGGGATTAACTCTTCTCGGTGAAAAGCTATTCCAAGTAACTTGGTTGACGCAAATTGGTTTCAAATATGACCGAAATGATTTCAGCAAATCTGAAAAGTTCACTCATCAAATGAAAGATGGTTGGGGATTGGCCACTGATGGAAAAGTGCTATTTGGGAGTGATGGCACGTCCATGTTGTACAAGATTGACCCTCAAACATTGAAAGTTATAGACAAGCACGTAGTCAAATACAAAGATCATGAAGTGCACAACCTCAATGAGTTGGAGTTTATAAAAGGCGAAGTTTGGGCAAATGTTTATCAGACAGACTGCATTGCTAGAATCTCGCAAGACGATGGTAGAGTGCTTGGATGGATTCTCCTTCCAAATTTAAGGGAGGGCTTAGTAGCTGCTGGGAATCATGCTATAGATGTGCTAAATGGCATTGCATGGGATAGCAACACCAACCGTATCTTTGTGACTGGCAAACTATGGCCAAAGCTTTATGAGATCAAGCTGCGTCCAATAAAGAAACAGTTCAAGGACGGAGCCATCAAGCAGCTATGCCTGCGGCAGACATTCTTTCGTTGA

Protein Analysis

325

Amino Acids

36.57

Weight (kDa)

9.49

Isoelectric Point (pI)

25.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_cyclase_2 PF05096 62 - 167 6.4e-35 Glutamine cyclotransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 677
Acc16I TGCGCA 1 cut(s) 338
Acc36I ACCTGC 1 cut(s) 315
AccB7I CCANNNNNTGG 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 146
AccII CGCG 1 cut(s) 203
AciI CCGC 3 cut(s) 144, 201, 955
AcoI YGGCCR 3 cut(s) 76, 534, 878
AcsI RAATTY 1 cut(s) 772
AcuI CTGAAG 2 cut(s) 142, 359
AcvI CACGTG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 1 cut(s) 584
AfiI CCNNNNNNNGG 2 cut(s) 177, 291
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 4 cut(s) 353, 466, 607, 925
AjiI CACGTC 1 cut(s) 573
AluBI AGCT 6 cut(s) 183, 430, 794, 886, 901, 946
AluI AGCT 6 cut(s) 183, 430, 794, 886, 901, 946
Alw21I GWGCWC 2 cut(s) 185, 654
Alw26I GTCTC 1 cut(s) 169
Alw44I GTGCAC 1 cut(s) 650
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 4 cut(s) 26, 76, 534, 878
ApaLI GTGCAC 1 cut(s) 650
ApeKI GCWGC 3 cut(s) 794, 901, 943
ApoI RAATTY 1 cut(s) 772
Asp700I GAANNNNTTC 1 cut(s) 920
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 27
AsuC2I CCSGG 3 cut(s) 75, 189, 190
AsuHPI GGTGA 2 cut(s) 177, 434
AvaI CYCGRG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 654
BalI TGGCCA 2 cut(s) 536, 880
BanII GRGCYC 1 cut(s) 185
BbrPI CACGTG 1 cut(s) 177
Bbv12I GWGCWC 2 cut(s) 185, 654
BbvI GCAGC 3 cut(s) 781, 888, 955
BccI CCATC 7 cut(s) 376, 515, 536, 560, 734, 750, 944
BceAI ACGGC 1 cut(s) 63
BcnI CCSGG 3 cut(s) 75, 189, 190
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 1 cut(s) 723
BfmI CTRYAG 1 cut(s) 810
BfuAI ACCTGC 1 cut(s) 315
BisI GCNGC 6 cut(s) 144, 201, 795, 902, 944, 956
BlsI GCNGC 6 cut(s) 145, 202, 796, 903, 945, 957
Bme1390I CCNGG 3 cut(s) 75, 189, 190
BmeT110I CYCGRG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 573
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 3 cut(s) 72, 296, 934
BmrFI CCNGG 3 cut(s) 75, 189, 190
BmrI ACTGGG 1 cut(s) 321
BmuI ACTGGG 1 cut(s) 321
BpuMI CCSGG 3 cut(s) 75, 189, 190
BsaAI YACGTR 2 cut(s) 177, 625
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 66, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 291
Bse1I ACTGG 2 cut(s) 316, 871
Bse3DI GCAATG 2 cut(s) 717, 828
BseDI CCNNGG 2 cut(s) 66, 188
BseGI GGATG 2 cut(s) 135, 761
BseLI CCNNNNNNNGG 2 cut(s) 177, 291
BseMI GCAATG 2 cut(s) 717, 828
BseMII CTCAG 1 cut(s) 281
BseNI ACTGG 2 cut(s) 316, 871
BseRI GAGGAG 2 cut(s) 113, 157
BseSI GKGCMC 1 cut(s) 654
BseX3I CGGCCG 1 cut(s) 76
BseXI GCAGC 3 cut(s) 781, 888, 955
BseYI CCCAGC 1 cut(s) 797
Bsh1236I CGCG 1 cut(s) 203
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 4 cut(s) 28, 78, 536, 880
BsiEI CGRYCG 1 cut(s) 79
BsiHKAI GWGCWC 2 cut(s) 185, 654
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 3 cut(s) 75, 189, 285
BslI CCNNNNNNNGG 2 cut(s) 177, 291
BsmAI GTCTC 1 cut(s) 169
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 4 cut(s) 28, 78, 536, 880
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 185, 654
Bsp1407I TGTACA 1 cut(s) 582
Bsp143I GATC 3 cut(s) 11, 640, 894
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 3 cut(s) 144, 201, 955
BspANI GGCC 4 cut(s) 28, 78, 536, 880
BspCNI CTCAG 1 cut(s) 280
BspFNI CGCG 1 cut(s) 203
BspHI TCATGA 2 cut(s) 247, 643
BspLI GGNNCC 3 cut(s) 72, 296, 934
BspMI ACCTGC 1 cut(s) 315
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 2 cut(s) 717, 828
BsrGI TGTACA 1 cut(s) 582
BsrI ACTGG 2 cut(s) 316, 871
BssECI CCNNGG 2 cut(s) 66, 188
BssMI GATC 3 cut(s) 11, 640, 894
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 66
Bst4CI ACNGT 3 cut(s) 334, 854, 921
Bst6I CTCTTC 2 cut(s) 161, 420
BstACI GRCGYC 1 cut(s) 198
BstAUI TGTACA 1 cut(s) 582
BstBAI YACGTR 2 cut(s) 177, 625
BstC8I GCNNGC 1 cut(s) 953
BstDEI CTNAG 2 cut(s) 267, 787
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 135, 761
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 3 cut(s) 14, 643, 897
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 3 cut(s) 11, 640, 894
BstMCI CGRYCG 1 cut(s) 79
BstMWI GCNNNNNNNGC 4 cut(s) 34, 149, 209, 952
BstSCI CCNGG 3 cut(s) 73, 187, 188
BstSFI CTRYAG 1 cut(s) 810
BstSLI GKGCMC 1 cut(s) 654
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 3 cut(s) 781, 888, 955
BstZI CGGCCG 1 cut(s) 76
BsuRI GGCC 4 cut(s) 28, 78, 536, 880
BtgI CCRYGG 1 cut(s) 66
BtrI CACGTC 1 cut(s) 573
BtsCI GGATG 2 cut(s) 135, 761
BtsIMutI CAGTG 3 cut(s) 309, 339, 537
BveI ACCTGC 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 953
CciI TCATGA 2 cut(s) 247, 643
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 27
Cfr9I CCCGGG 1 cut(s) 188
CseI GACGC 4 cut(s) 151, 206, 460, 893
Csp6I GTAC 1 cut(s) 583
CviAII CATG 7 cut(s) 67, 127, 248, 577, 644, 806, 834
CviQI GTAC 1 cut(s) 583
DdeI CTNAG 2 cut(s) 267, 787
DpnI GATC 3 cut(s) 13, 642, 896
DpnII GATC 3 cut(s) 11, 640, 894
EaeI YGGCCR 3 cut(s) 76, 534, 878
EagI CGGCCG 1 cut(s) 76
Eam1104I CTCTTC 2 cut(s) 161, 420
EarI CTCTTC 2 cut(s) 161, 420
Ecl136II GAGCTC 1 cut(s) 183
EclXI CGGCCG 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 185
Eco52I CGGCCG 1 cut(s) 76
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 2 cut(s) 142, 359
Eco72I CACGTG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 7 cut(s) 70, 130, 251, 580, 647, 809, 837
FatI CATG 7 cut(s) 66, 126, 247, 576, 643, 805, 833
Fnu4HI GCNGC 6 cut(s) 144, 201, 795, 902, 944, 956
FokI GGATG 2 cut(s) 122, 768
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 6 cut(s) 144, 201, 795, 902, 944, 956
FspBI CTAG 1 cut(s) 723
FspI TGCGCA 1 cut(s) 338
GlaI GCGC 1 cut(s) 338
GluI GCNGC 6 cut(s) 144, 201, 795, 902, 944, 956
GsaI CCCAGC 1 cut(s) 801
HaeIII GGCC 4 cut(s) 28, 78, 536, 880
HapII CCGG 3 cut(s) 75, 189, 285
HgaI GACGC 4 cut(s) 151, 206, 460, 893
HhaI GCGC 1 cut(s) 339
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 7 cut(s) 70, 130, 251, 580, 647, 809, 837
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HincII GTYRAC 2 cut(s) 330, 450
HindII GTYRAC 2 cut(s) 330, 450
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 5 cut(s) 305, 386, 726, 760, 802
HpaII CCGG 3 cut(s) 75, 189, 285
HphI GGTGA 2 cut(s) 177, 434
Hpy166II GTNNAC 6 cut(s) 217, 309, 330, 450, 504, 652
Hpy188I TCNGA 2 cut(s) 496, 708
Hpy188III TCNNGA 3 cut(s) 248, 377, 644
Hpy8I GTNNAC 6 cut(s) 217, 309, 330, 450, 504, 652
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 2 cut(s) 271, 776
HpyCH4III ACNGT 3 cut(s) 334, 854, 921
HpyCH4IV ACGT 3 cut(s) 176, 572, 624
HpyCH4V TGCA 3 cut(s) 652, 717, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 149, 209, 952
HpyF3I CTNAG 2 cut(s) 267, 787
HpySE526I ACGT 3 cut(s) 176, 572, 624
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 7 cut(s) 70, 130, 251, 580, 647, 809, 837
HspAI GCGC 1 cut(s) 337
Kzo9I GATC 3 cut(s) 11, 640, 894
LmnI GCTCC 4 cut(s) 76, 180, 294, 932
Lsp1109I GCAGC 3 cut(s) 781, 888, 955
MaeI CTAG 1 cut(s) 723
MaeII ACGT 3 cut(s) 176, 572, 624
MaeIII GTNAC 2 cut(s) 439, 862
MalI GATC 3 cut(s) 13, 642, 896
MbiI CCGCTC 1 cut(s) 146
MboI GATC 3 cut(s) 11, 640, 894
MboII GAAGA 3 cut(s) 148, 365, 407
MhlI GDGCHC 2 cut(s) 185, 654
MlsI TGGCCA 2 cut(s) 536, 880
MluCI AATT 2 cut(s) 456, 772
MluNI TGGCCA 2 cut(s) 536, 880
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 2 cut(s) 195, 648
Mox20I TGGCCA 2 cut(s) 536, 880
MroXI GAANNNNTTC 1 cut(s) 920
MscI TGGCCA 2 cut(s) 536, 880
MseI TTAA 4 cut(s) 207, 234, 410, 776
MslI CAYNNNNRTG 2 cut(s) 65, 575
Msp20I TGGCCA 2 cut(s) 536, 880
MspI CCGG 3 cut(s) 75, 189, 285
MspR9I CCNGG 3 cut(s) 75, 189, 190
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 34, 149, 209, 952
NciI CCSGG 3 cut(s) 75, 189, 190
NcoI CCATGG 1 cut(s) 66
NdeII GATC 3 cut(s) 11, 640, 894
NlaIII CATG 7 cut(s) 70, 130, 251, 580, 647, 809, 837
NlaIV GGNNCC 3 cut(s) 72, 296, 934
NmuCI GTSAC 1 cut(s) 862
NsbI TGCGCA 1 cut(s) 338
PagI TCATGA 2 cut(s) 247, 643
PdmI GAANNNNTTC 1 cut(s) 920
PfeI GAWTC 4 cut(s) 386, 726, 760, 802
PflMI CCANNNNNTGG 1 cut(s) 177
PkrI GCNGC 6 cut(s) 145, 202, 796, 903, 945, 957
PleI GAGTC 1 cut(s) 313
PmaCI CACGTG 1 cut(s) 177
PmlI CACGTG 1 cut(s) 177
PpsI GAGTC 1 cut(s) 313
Ppu21I YACGTR 2 cut(s) 177, 625
PsiI TTATAA 1 cut(s) 677
Psp124BI GAGCTC 1 cut(s) 185
PspCI CACGTG 1 cut(s) 177
PspFI CCCAGC 1 cut(s) 797
PspN4I GGNNCC 3 cut(s) 72, 296, 934
PspPI GGNCC 1 cut(s) 27
RsaI GTAC 1 cut(s) 584
RsaNI GTAC 1 cut(s) 583
RseI CAYNNNNRTG 2 cut(s) 65, 575
SacI GAGCTC 1 cut(s) 185
SaqAI TTAA 4 cut(s) 207, 234, 410, 776
SatI GCNGC 6 cut(s) 144, 201, 795, 902, 944, 956
Sau3AI GATC 3 cut(s) 11, 640, 894
Sau96I GGNCC 1 cut(s) 27
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 75, 189, 190
SduI GDGCHC 2 cut(s) 185, 654
SfcI CTRYAG 1 cut(s) 810
SmaI CCCGGG 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 65, 575
Sse9I AATT 2 cut(s) 456, 772
SsiI CCGC 3 cut(s) 144, 201, 955
SspI AATATT 1 cut(s) 395
SspMI CTAG 1 cut(s) 723
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 3 cut(s) 73, 187, 188
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 3 cut(s) 334, 854, 921
TaiI ACGT 3 cut(s) 179, 575, 627
TaqII GACCGA 1 cut(s) 489
TasI AATT 2 cut(s) 456, 772
TatI WGTACW 1 cut(s) 582
TauI GCSGC 3 cut(s) 146, 203, 958
TfiI GAWTC 4 cut(s) 386, 726, 760, 802
Tru1I TTAA 4 cut(s) 207, 234, 410, 776
Tru9I TTAA 4 cut(s) 207, 234, 410, 776
TscAI CASTG 3 cut(s) 316, 339, 544
TseFI GTSAC 1 cut(s) 862
TseI GCWGC 3 cut(s) 794, 901, 943
Tsp45I GTSAC 1 cut(s) 862
TspDTI ATGAA 2 cut(s) 530, 660
TspGWI ACGGA 1 cut(s) 945
TspMI CCCGGG 1 cut(s) 188
TspRI CASTG 3 cut(s) 316, 339, 544
Van91I CCANNNNNTGG 1 cut(s) 177
VneI GTGCAC 1 cut(s) 650
XapI RAATTY 1 cut(s) 772
XmaI CCCGGG 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 920
XspI CTAG 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.