MD01G1208600.v1.1

Glutaminyl-peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007377
Physical Location & Seq
Reverse (-)
30311475 .. 30314296
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1208600.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGCCACCGGTTCGCTTAGGAAGAAGCTGAATAACAATAAGCGGTCCGACCGCAGGCCATACAATAACGCTCCCATGGCGGCCTCTCGGCCCAGGTCGCTTCCGAACTACAGGAAAACCTCTCTGATCCTCGCCGCTTTTCTCGTGCTCGGCGTTCTTATTCTCCTCGGCATTTCGTCGAACATGTGGAGCACCGTCATCTACGCCGACGTCTCCCCTGGACTTTACTCGATCGAGGTCGTCCATGAGTTCCCTCACGATCCCGACGCCTTCACCCAGGGGCTACTTTATGCTGGAAATGACAGCTTGTTCGAGTCGACTGGACTTTACGGGAAGTCATCAGTGCGCAAGGTTGCGATTCGAACCGGAAAGGTTGAAGCACTTCAGAAGATGGACCCTTCTTACTTTGGGGAGGGATTAACTCTCCTTGGTGAAAGGCTATTCCAAGTAACTTGGTTGAGGAAAATTGGTTTCATATATGACCGAAAAAATTTAACCAAATTTGAGAAGTTCACGCATCAGATGAAAGATGGCTGGGGACTGGCTACTGATGGAAAAGTGTTGTTTGGAAGTGATGGCAGTTCAGTGTTGTACAAGATTGACCCTCTAACATTAAAAGTTACGGACAGACATATGGTCAAATATAAAGATCAAGAAGTGCACAACCTCAACGAGTTAGAGTTTATAAACGGCGAAGTTTGGGCAAATGTATATCAGACAGATTGCATAGCTAGAATCTCGCATGAAGATGGTAGGGTGCTTGGATGGATCGTCCTTCCAAAATTAAGGGAAGGCTTACTAGCTTCTGGGAATCATGGTATAGATGTTCTAAACGGCATTGCATGGGACAGTGACAAAAACCGTATATTTGTGACCGGAAAACTATGGCCAAAGCTTTACGAGATCAAGCTACATCCGATGAAGAAACAGTTCAAAGACGGGGCCATTGAGCGGCTTTGCATGCGGAGGCCATTCAATTTTTGA

Protein Analysis

328

Amino Acids

37.14

Weight (kDa)

9.52

Isoelectric Point (pI)

29.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_cyclase_2 PF05096 71 - 304 2.4e-92 Glutamine cyclotransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 686
AatII GACGTC 1 cut(s) 213
Acc16I TGCGCA 1 cut(s) 347
AccBSI CCGCTC 1 cut(s) 952
AccI GTMKAC 1 cut(s) 317
AciI CCGC 6 cut(s) 43, 52, 80, 135, 952, 964
AclWI GGATC 3 cut(s) 121, 254, 776
AcoI YGGCCR 2 cut(s) 3, 887
AcsI RAATTY 2 cut(s) 490, 500
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 2 cut(s) 210, 267
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 2 cut(s) 54, 951
AflIII ACRYGT 1 cut(s) 183
AgeI ACCGGT 1 cut(s) 8
AgsI TTSAA 3 cut(s) 377, 934, 976
AhdI GACNNNNNGTC 1 cut(s) 635
AjnI CCWGG 3 cut(s) 92, 217, 276
AluBI AGCT 6 cut(s) 28, 306, 731, 803, 895, 910
AluI AGCT 6 cut(s) 28, 306, 731, 803, 895, 910
Alw21I GWGCWC 3 cut(s) 150, 194, 663
Alw26I GTCTC 1 cut(s) 217
Alw44I GTGCAC 1 cut(s) 659
AlwI GGATC 3 cut(s) 121, 254, 776
AoxI GGCC 7 cut(s) 3, 56, 81, 89, 887, 942, 968
ApaLI GTGCAC 1 cut(s) 659
ApoI RAATTY 2 cut(s) 490, 500
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AsiGI ACCGGT 1 cut(s) 8
Asp700I GAANNNNTTC 2 cut(s) 381, 929
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 4 cut(s) 45, 90, 394, 942
AsuHPI GGTGA 2 cut(s) 265, 443
AsuII TTCGAA 1 cut(s) 361
AvaII GGWCC 2 cut(s) 45, 394
BaeGI GKGCMC 1 cut(s) 663
BalI TGGCCA 2 cut(s) 5, 889
BauI CACGAG 1 cut(s) 143
Bbv12I GWGCWC 3 cut(s) 150, 194, 663
BccI CCATC 6 cut(s) 385, 524, 545, 569, 743, 759
BceAI ACGGC 2 cut(s) 706, 850
BciT130I CCWGG 3 cut(s) 94, 219, 278
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 2 cut(s) 732, 800
BfmI CTRYAG 1 cut(s) 109
BisI GCNGC 3 cut(s) 81, 135, 953
BlsI GCNGC 3 cut(s) 82, 136, 954
Bme1390I CCNGG 3 cut(s) 94, 219, 278
Bme18I GGWCC 2 cut(s) 45, 394
BmeRI GACNNNNNGTC 1 cut(s) 635
BmgT120I GGNCC 4 cut(s) 45, 90, 394, 942
BmiI GGNNCC 2 cut(s) 396, 943
BmrFI CCNGG 3 cut(s) 94, 219, 278
BmsI GCATC 1 cut(s) 526
Bpu10I CCTNAGC 1 cut(s) 17
Bpu14I TTCGAA 1 cut(s) 361
BsaHI GRCGYC 2 cut(s) 210, 267
BsaJI CCNNGG 7 cut(s) 75, 92, 166, 217, 276, 277, 427
BsaWI WCCGGW 3 cut(s) 8, 365, 875
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 951
Bse118I RCCGGY 1 cut(s) 8
Bse1I ACTGG 2 cut(s) 325, 546
Bse3DI GCAATG 1 cut(s) 837
BseBI CCWGG 3 cut(s) 94, 219, 278
BseDI CCNNGG 7 cut(s) 75, 92, 166, 217, 276, 277, 427
BseGI GGATG 2 cut(s) 770, 913
BseLI CCNNNNNNNGG 2 cut(s) 54, 951
BseMI GCAATG 1 cut(s) 837
BseNI ACTGG 2 cut(s) 325, 546
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 663
BseYI CCCAGC 1 cut(s) 534
Bsh1285I CGRYCG 2 cut(s) 52, 234
BshFI GGCC 7 cut(s) 5, 58, 83, 91, 889, 944, 970
BshTI ACCGGT 1 cut(s) 8
BsiEI CGRYCG 2 cut(s) 52, 234
BsiHKAI GWGCWC 3 cut(s) 150, 194, 663
BsiSI CCGG 3 cut(s) 9, 366, 876
BslFI GGGAC 2 cut(s) 552, 860
BslI CCNNNNNNNGG 2 cut(s) 54, 951
BsmAI GTCTC 1 cut(s) 217
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 2 cut(s) 552, 860
BsnI GGCC 7 cut(s) 5, 58, 83, 91, 889, 944, 970
Bsp119I TTCGAA 1 cut(s) 361
Bsp1286I GDGCHC 3 cut(s) 150, 194, 663
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 6 cut(s) 126, 231, 259, 649, 768, 903
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 6 cut(s) 43, 52, 80, 135, 952, 964
BspANI GGCC 7 cut(s) 5, 58, 83, 91, 889, 944, 970
BspLI GGNNCC 2 cut(s) 396, 943
BspPI GGATC 3 cut(s) 121, 254, 776
BspT104I TTCGAA 1 cut(s) 361
BsrBI CCGCTC 1 cut(s) 952
BsrDI GCAATG 1 cut(s) 837
BsrFI RCCGGY 1 cut(s) 8
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 2 cut(s) 325, 546
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 7 cut(s) 75, 92, 166, 217, 276, 277, 427
BssMI GATC 6 cut(s) 126, 231, 259, 649, 768, 903
BssNI GRCGYC 2 cut(s) 210, 267
BssSI CACGAG 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 75, 427
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 3 cut(s) 94, 219, 278
Bst4CI ACNGT 4 cut(s) 196, 851, 863, 930
BstACI GRCGYC 2 cut(s) 210, 267
BstAUI TGTACA 1 cut(s) 591
BstBI TTCGAA 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 56, 962
BstDEI CTNAG 1 cut(s) 17
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 2 cut(s) 770, 913
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 6 cut(s) 129, 234, 262, 652, 771, 906
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 6 cut(s) 126, 231, 259, 649, 768, 903
BstMCI CGRYCG 2 cut(s) 52, 234
BstMWI GCNNNNNNNGC 3 cut(s) 77, 97, 961
BstNI CCWGG 3 cut(s) 94, 219, 278
BstNSI RCATGY 2 cut(s) 187, 964
BstSCI CCNGG 3 cut(s) 92, 217, 276
BstSFI CTRYAG 1 cut(s) 109
BstSLI GKGCMC 1 cut(s) 663
BsuRI GGCC 7 cut(s) 5, 58, 83, 91, 889, 944, 970
BtgI CCRYGG 1 cut(s) 75
BtsCI GGATG 2 cut(s) 770, 913
BtsIMutI CAGTG 3 cut(s) 348, 591, 856
Cac8I GCNNGC 2 cut(s) 56, 962
CfoI GCGC 1 cut(s) 348
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 4 cut(s) 45, 90, 394, 942
CpoI CGGWCCG 1 cut(s) 45
CseI GACGC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 592
CspAI ACCGGT 1 cut(s) 8
CspI CGGWCCG 1 cut(s) 45
CviAII CATG 7 cut(s) 76, 184, 245, 743, 815, 843, 961
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 1 cut(s) 17
DpnI GATC 6 cut(s) 128, 233, 261, 651, 770, 905
DpnII GATC 6 cut(s) 126, 231, 259, 649, 768, 903
DriI GACNNNNNGTC 1 cut(s) 635
EaeI YGGCCR 2 cut(s) 3, 887
Eam1105I GACNNNNNGTC 1 cut(s) 635
Eco130I CCWWGG 2 cut(s) 75, 427
Eco47I GGWCC 2 cut(s) 45, 394
Eco57I CTGAAG 1 cut(s) 368
EcoRII CCWGG 3 cut(s) 92, 217, 276
EcoT14I CCWWGG 2 cut(s) 75, 427
ErhI CCWWGG 2 cut(s) 75, 427
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 7 cut(s) 79, 187, 248, 746, 818, 846, 964
FaqI GGGAC 2 cut(s) 552, 860
FatI CATG 7 cut(s) 75, 183, 244, 742, 814, 842, 960
FauNDI CATATG 1 cut(s) 633
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 3 cut(s) 81, 135, 953
FokI GGATG 2 cut(s) 777, 900
Fsp4HI GCNGC 3 cut(s) 81, 135, 953
FspBI CTAG 2 cut(s) 732, 800
FspI TGCGCA 1 cut(s) 347
GlaI GCGC 1 cut(s) 347
GluI GCNGC 3 cut(s) 81, 135, 953
GsaI CCCAGC 1 cut(s) 538
HaeIII GGCC 7 cut(s) 5, 58, 83, 91, 889, 944, 970
HapII CCGG 3 cut(s) 9, 366, 876
HgaI GACGC 1 cut(s) 275
HhaI GCGC 1 cut(s) 348
Hin1I GRCGYC 2 cut(s) 210, 267
Hin1II CATG 7 cut(s) 79, 187, 248, 746, 818, 846, 964
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 4 cut(s) 314, 358, 735, 811
HpaII CCGG 3 cut(s) 9, 366, 876
HphI GGTGA 2 cut(s) 265, 443
Hpy166II GTNNAC 3 cut(s) 318, 513, 661
Hpy188I TCNGA 7 cut(s) 49, 105, 126, 387, 522, 717, 918
Hpy188III TCNNGA 3 cut(s) 257, 263, 653
Hpy8I GTNNAC 3 cut(s) 318, 513, 661
Hpy99I CGWCG 3 cut(s) 181, 212, 269
HpyAV CCTTC 4 cut(s) 280, 408, 785, 785
HpyCH4III ACNGT 4 cut(s) 196, 851, 863, 930
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 4 cut(s) 661, 726, 842, 960
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 97, 961
HpyF3I CTNAG 1 cut(s) 17
HpySE526I ACGT 1 cut(s) 210
Hsp92I GRCGYC 2 cut(s) 210, 267
Hsp92II CATG 7 cut(s) 79, 187, 248, 746, 818, 846, 964
HspAI GCGC 1 cut(s) 346
Kzo9I GATC 6 cut(s) 126, 231, 259, 649, 768, 903
LmnI GCTCC 2 cut(s) 76, 189
LweI GCATC 1 cut(s) 526
MaeI CTAG 2 cut(s) 732, 800
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 4 cut(s) 448, 619, 851, 871
MalI GATC 6 cut(s) 128, 233, 261, 651, 770, 905
MbiI CCGCTC 1 cut(s) 952
MboI GATC 6 cut(s) 126, 231, 259, 649, 768, 903
MboII GAAGA 4 cut(s) 34, 400, 758, 934
MhlI GDGCHC 3 cut(s) 150, 194, 663
MlsI TGGCCA 2 cut(s) 5, 889
MluCI AATT 5 cut(s) 465, 490, 500, 782, 976
MluNI TGGCCA 2 cut(s) 5, 889
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 72
Mox20I TGGCCA 2 cut(s) 5, 889
MroXI GAANNNNTTC 2 cut(s) 381, 929
MscI TGGCCA 2 cut(s) 5, 889
MseI TTAA 4 cut(s) 419, 494, 614, 785
MslI CAYNNNNRTG 1 cut(s) 747
Msp20I TGGCCA 2 cut(s) 5, 889
MspI CCGG 3 cut(s) 9, 366, 876
MspR9I CCNGG 3 cut(s) 94, 219, 278
MvaI CCWGG 3 cut(s) 94, 219, 278
MwoI GCNNNNNNNGC 3 cut(s) 77, 97, 961
NcoI CCATGG 1 cut(s) 75
NdeI CATATG 1 cut(s) 633
NdeII GATC 6 cut(s) 126, 231, 259, 649, 768, 903
NlaIII CATG 7 cut(s) 79, 187, 248, 746, 818, 846, 964
NlaIV GGNNCC 2 cut(s) 396, 943
NmeAIII GCCGAG 3 cut(s) 67, 129, 147
NmuCI GTSAC 2 cut(s) 851, 871
NsbI TGCGCA 1 cut(s) 347
NspI RCATGY 2 cut(s) 187, 964
NspV TTCGAA 1 cut(s) 361
PaeI GCATGC 1 cut(s) 964
PasI CCCWGGG 1 cut(s) 277
PciI ACATGT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdmI GAANNNNTTC 2 cut(s) 381, 929
PfeI GAWTC 3 cut(s) 358, 735, 811
PinAI ACCGGT 1 cut(s) 8
PkrI GCNGC 3 cut(s) 82, 136, 954
Ple19I CGATCG 1 cut(s) 234
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PscI ACATGT 1 cut(s) 183
PsiI TTATAA 1 cut(s) 686
Psp6I CCWGG 3 cut(s) 92, 217, 276
PspFI CCCAGC 1 cut(s) 534
PspGI CCWGG 3 cut(s) 92, 217, 276
PspN4I GGNNCC 2 cut(s) 396, 943
PspPI GGNCC 4 cut(s) 45, 90, 394, 942
PvuI CGATCG 1 cut(s) 234
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 1 cut(s) 747
Rsr2I CGGWCCG 1 cut(s) 45
RsrII CGGWCCG 1 cut(s) 45
SalI GTCGAC 1 cut(s) 316
SaqAI TTAA 4 cut(s) 419, 494, 614, 785
SatI GCNGC 3 cut(s) 81, 135, 953
Sau3AI GATC 6 cut(s) 126, 231, 259, 649, 768, 903
Sau96I GGNCC 4 cut(s) 45, 90, 394, 942
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 3 cut(s) 94, 219, 278
SduI GDGCHC 3 cut(s) 150, 194, 663
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 109
SfuI TTCGAA 1 cut(s) 361
SinI GGWCC 2 cut(s) 45, 394
SmiMI CAYNNNNRTG 1 cut(s) 747
SphI GCATGC 1 cut(s) 964
Sse9I AATT 5 cut(s) 465, 490, 500, 782, 976
SsiI CCGC 6 cut(s) 43, 52, 80, 135, 952, 964
SspMI CTAG 2 cut(s) 732, 800
StyD4I CCNGG 3 cut(s) 92, 217, 276
StyI CCWWGG 2 cut(s) 75, 427
TaaI ACNGT 4 cut(s) 196, 851, 863, 930
TaiI ACGT 1 cut(s) 213
TaqI TCGA 6 cut(s) 179, 230, 234, 312, 317, 361
TaqII GACCGA 1 cut(s) 498
TasI AATT 5 cut(s) 465, 490, 500, 782, 976
TatI WGTACW 1 cut(s) 591
TauI GCSGC 3 cut(s) 83, 137, 955
TfiI GAWTC 3 cut(s) 358, 735, 811
Tru1I TTAA 4 cut(s) 419, 494, 614, 785
Tru9I TTAA 4 cut(s) 419, 494, 614, 785
TscAI CASTG 3 cut(s) 348, 591, 856
TseFI GTSAC 2 cut(s) 851, 871
Tsp45I GTSAC 2 cut(s) 851, 871
TspDTI ATGAA 4 cut(s) 463, 539, 759, 935
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 3 cut(s) 348, 591, 856
VneI GTGCAC 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 45, 394
XapI RAATTY 2 cut(s) 490, 500
XceI RCATGY 2 cut(s) 187, 964
XmiI GTMKAC 1 cut(s) 317
XmnI GAANNNNTTC 2 cut(s) 381, 929
XspI CTAG 2 cut(s) 732, 800
ZraI GACGTC 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.