AT4G25835

ATPase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013397
Physical Location & Seq
Forward (+)
13135693 .. 13137923
2231 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25835.1

Sequence Viewer

Length: 1521 bp
ATGAAAGAGTATTGGACATCTTTGGCTTCTCTACTCGGCGTTTTAGCCTTTTGCCAAAGCCTAATGAATTCTGTATTCCCACCAGAGCTCCGTTTCGCCATCTCGAAACTCTTCAACAAATTCTTTAAACTCTTCTCCACCTTCTGTTACTTCGACATAACAGAGATTGACGGCGTCAACACCAACGAGCTTTACAATGCCGTCCAGCTTTACCTCAGCTCCTCTGTCTCCATCGCAGGTAACCGTCTCAGCCTCACACGCGCCGTCAACTCCTCCTCCGTCACATTCGGTCTCTCCAACAACGACTCCATCGTCGACACGTTTAACTCCGTCACCGTCGTCTGGGAACACATCGTTACACAGAGACAAACTCAAACCTTCGCTTGGAGACCAATGCCGGAAGAGAAACGTGGCTTCACGCTCCGGATCAAGAAGAAAGACAAGAACTTGATCCTTGATTCTTACTTGGATTACATCATGGAGAAAGCTAACGAGATTCGTCGGTTGAATCAAGATCGGCTTCTTTACACGAATTCACGAGGTGGGTCTCTTGATTCTAGAGGTCTTCCTTGGGAATCTGTTCCCTTTAAGCATCCGAGTACTTTCGATACTCTCGCTATGGATCCGGTTAAGAAGCAACAGATCATGGAGGATCTTAAAGATTTCGCTGAGTGTCAATCTTTTTACGAGAGAACTGGTCGGGCTTGGAAAAGAGGTTACTTGCTCTATGGACCTCCCGGAACAGGGAAGTCGAGCATGATCGCCGCCATGGCTAACTATCTCCGGTACGACATCTACGATCTTGAGCTCACGGAGGTCAAAAGCAATTCTGAACTGAGGAAGCTTCTGATGAAGACTAGCTCCAAGTCGATAATTGTTATCGAGGATATTGATTGTTCTATCAATTTAACGAATCGTAACAAGAAACAGAGCACAGGGAGCTATAACGAACCGGAGATGTTAACCGGGTCCGGTTTAGGTGATGATTTGGGTGATGGGAACACTATAACGCTTTCAGGTTTGTTGAATTTCACTGATGGACTTTGGTCTTGTTGTGGTAGCGAGAGGATTTTTGTGTTCACGACGAATCACATTGAGAAGCTTGATCCTGCATTGCTTAGAAGTGGAAGAATGGATATGCATATACACATGAGTTACTGTACGTTTTCTTCGGTCAAGATTTTATTGAGGAATTACTTGGGGTTTGAGGAAGGTGATCTTAACGACGTTGTTTTGAAGGAGTTAGCGGAAGTGGTTGATAGGGCTGAGATTACACCGGCTGACGTAAGTGAGGCTTTGATTAAGAATCGGAGGGATAAAGAGAGAGCTGTGAGGGAGCTTTTGGTTGATTTGAGAAGTAGAGTGGAGAGAAATGAGAAGAATGGGAAATCGAGGGTTCAAAATGTGAGCTTGGAAGAGCAAGAGAATAGAGCTTTCGACAGTCTTTACGCAGAAGAGAATGGAGGAGAAGAAGAAGAGATTGAGGATAACATTTGCAAAAGTAGTGATGATTGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

506

Amino Acids

57.61

Weight (kDa)

5.24

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 26 - 118 2.6e-22 Domain associated at C-terminal with AAA
AAA PF00004 240 - 385 7.8e-18 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 387 - 459 7e-10 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25835 AT4G30250 AT4G30250 AT5G57480
fragaria_vesca FvH4_4g08330
malus_domestica MD13G1255400.v1.1
prunus_persica Prupe.1G086500_v2.0.a1
pyrus_communis pycom13g22720
rosa_chinensis RchiOBHm_Chr4g0402591
rosa_laevigata RLG00000009064
rosa_multiflora Rmu_co8332339.1_g000001 Rmu_sc0004921.1_g000012
rosa_roxburghii Rroxscaffold_5G00347310
rosa_rugosa Rorug04G0036900
rosa_samantha Rh4AG114500 Rh4BG106800 Rh4DG106700
rosa_wichuraiana Rw4G009130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 227
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 423
AciI CCGC 2 cut(s) 765, 1247
AclWI GGATC 6 cut(s) 434, 445, 617, 630, 660, 1100
AcsI RAATTY 4 cut(s) 67, 119, 532, 1027
AcyI GRCGYC 1 cut(s) 174
AdeI CACNNNGTG 1 cut(s) 542
AfaI GTAC 3 cut(s) 601, 788, 1162
AfiI CCNNNNNNNGG 4 cut(s) 342, 384, 743, 744
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 5 cut(s) 115, 508, 1027, 1237, 1400
Alw21I GWGCWC 3 cut(s) 90, 810, 935
Alw26I GTCTC 6 cut(s) 232, 251, 296, 358, 382, 552
AlwI GGATC 6 cut(s) 434, 445, 617, 630, 660, 1100
Aor13HI TCCGGA 1 cut(s) 423
ApoI RAATTY 4 cut(s) 67, 119, 532, 1027
Asp700I GAANNNNTTC 2 cut(s) 110, 579
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 2 cut(s) 731, 969
AsuC2I CCSGG 2 cut(s) 738, 967
AsuHPI GGTGA 4 cut(s) 325, 992, 1004, 1226
AvaII GGWCC 2 cut(s) 731, 969
BamHI GGATCC 1 cut(s) 622
BanII GRGCYC 2 cut(s) 90, 810
BarI GAAGNNNNNNTAC 2 cut(s) 1153, 1185
BauI CACGAG 1 cut(s) 537
BbsI GAAGAC 2 cut(s) 557, 860
Bbv12I GWGCWC 3 cut(s) 90, 810, 935
BbvCI CCTCAGC 1 cut(s) 215
BccI CCATC 5 cut(s) 107, 239, 317, 989, 1031
BceAI ACGGC 3 cut(s) 185, 187, 248
BcnI CCSGG 2 cut(s) 738, 967
BcoDI GTCTC 6 cut(s) 232, 251, 296, 358, 382, 552
BfaI CTAG 2 cut(s) 558, 858
BfuAI ACCTGC 1 cut(s) 227
BglI GCCNNNNNGGC 1 cut(s) 770
BisI GCNGC 1 cut(s) 765
BlsI GCNGC 1 cut(s) 766
BmcAI AGTACT 1 cut(s) 601
Bme1390I CCNGG 2 cut(s) 738, 967
Bme18I GGWCC 2 cut(s) 731, 969
BmgT120I GGNCC 2 cut(s) 731, 969
BmiI GGNNCC 2 cut(s) 624, 970
BmrFI CCNGG 2 cut(s) 738, 967
BmsI GCATC 1 cut(s) 601
BoxI GACNNNNGTC 1 cut(s) 1045
BpiI GAAGAC 2 cut(s) 557, 860
BplI GAGNNNNNCTC 2 cut(s) 355, 387
Bpu10I CCTNAGC 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 824
BpuMI CCSGG 2 cut(s) 738, 967
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 3 cut(s) 296, 382, 552
BsaJI CCNNGG 2 cut(s) 569, 768
BsaWI WCCGGW 5 cut(s) 423, 625, 783, 952, 971
BsaXI ACNNNNNCTCC 8 cut(s) 72, 102, 203, 233, 260, 290, 290, 320
Bsc4I CCNNNNNNNGG 4 cut(s) 342, 384, 743, 744
Bse118I RCCGGY 1 cut(s) 1276
Bse1I ACTGG 1 cut(s) 700
Bse3DI GCAATG 1 cut(s) 1112
BseAI TCCGGA 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 569, 768
BseGI GGATG 1 cut(s) 592
BseLI CCNNNNNNNGG 4 cut(s) 342, 384, 743, 744
BseMI GCAATG 1 cut(s) 1112
BseMII CTCAG 5 cut(s) 229, 262, 660, 827, 1257
BseNI ACTGG 1 cut(s) 700
BseRI GAGGAG 4 cut(s) 211, 262, 265, 1479
Bsh1236I CGCG 1 cut(s) 261
BsiHKAI GWGCWC 3 cut(s) 90, 810, 935
BsiSI CCGG 9 cut(s) 398, 424, 626, 738, 784, 953, 966, 972, 1277
BslI CCNNNNNNNGG 4 cut(s) 342, 384, 743, 744
BsmAI GTCTC 6 cut(s) 232, 251, 296, 358, 382, 552
BsmBI CGTCTC 1 cut(s) 251
Bso31I GGTCTC 3 cut(s) 296, 382, 552
Bsp1286I GDGCHC 3 cut(s) 90, 810, 935
Bsp13I TCCGGA 1 cut(s) 423
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 2 cut(s) 765, 1247
BspCNI CTCAG 5 cut(s) 228, 261, 661, 828, 1258
BspEI TCCGGA 1 cut(s) 423
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 2 cut(s) 624, 970
BspMI ACCTGC 1 cut(s) 227
BspPI GGATC 6 cut(s) 434, 445, 617, 630, 660, 1100
BspQI GCTCTTC 1 cut(s) 1410
BspTNI GGTCTC 3 cut(s) 296, 382, 552
BsrDI GCAATG 1 cut(s) 1112
BsrFI RCCGGY 1 cut(s) 1276
BsrI ACTGG 1 cut(s) 700
BssAI RCCGGY 1 cut(s) 1276
BssECI CCNNGG 2 cut(s) 569, 768
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 2 cut(s) 569, 768
Bst2BI CACGAG 1 cut(s) 537
Bst4CI ACNGT 4 cut(s) 245, 337, 1160, 1442
Bst6I CTCTTC 6 cut(s) 116, 137, 396, 1410, 1449, 1470
BstACI GRCGYC 1 cut(s) 174
BstDEI CTNAG 6 cut(s) 215, 248, 669, 836, 1118, 1266
BstDSI CCRYGG 1 cut(s) 768
BstEII GGTNACC 1 cut(s) 239
BstF5I GGATG 1 cut(s) 592
BstFNI CGCG 1 cut(s) 261
BstHHI GCGC 1 cut(s) 263
BstMAI GTCTC 6 cut(s) 232, 251, 296, 358, 382, 552
BstMWI GCNNNNNNNGC 3 cut(s) 258, 770, 939
BstPAI GACNNNNGTC 1 cut(s) 1045
BstPI GGTNACC 1 cut(s) 239
BstSCI CCNGG 2 cut(s) 736, 965
BstUI CGCG 1 cut(s) 261
BstV2I GAAGAC 2 cut(s) 557, 860
BstX2I RGATCY 2 cut(s) 622, 652
BstYI RGATCY 2 cut(s) 622, 652
BtgI CCRYGG 1 cut(s) 768
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 1 cut(s) 592
BtsIMutI CAGTG 1 cut(s) 1032
BveI ACCTGC 1 cut(s) 227
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 1276
Cfr13I GGNCC 2 cut(s) 731, 969
CseI GACGC 1 cut(s) 163
Csp6I GTAC 3 cut(s) 600, 787, 1161
CviAII CATG 5 cut(s) 478, 646, 757, 769, 1150
CviQI GTAC 3 cut(s) 600, 787, 1161
DdeI CTNAG 6 cut(s) 215, 248, 669, 836, 1118, 1266
DraI TTTAAA 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 542
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
Eam1104I CTCTTC 6 cut(s) 116, 137, 396, 1410, 1449, 1470
EarI CTCTTC 6 cut(s) 116, 137, 396, 1410, 1449, 1470
Ecl136II GAGCTC 2 cut(s) 88, 808
Eco130I CCWWGG 2 cut(s) 569, 768
Eco24I GRGCYC 2 cut(s) 90, 810
Eco31I GGTCTC 3 cut(s) 296, 382, 552
Eco47I GGWCC 2 cut(s) 731, 969
Eco53kI GAGCTC 2 cut(s) 88, 808
Eco91I GGTNACC 1 cut(s) 239
EcoICRI GAGCTC 2 cut(s) 88, 808
EcoO65I GGTNACC 1 cut(s) 239
EcoRI GAATTC 2 cut(s) 67, 532
EcoT14I CCWWGG 2 cut(s) 569, 768
EcoT22I ATGCAT 1 cut(s) 1143
EcoT38I GRGCYC 2 cut(s) 90, 810
ErhI CCWWGG 2 cut(s) 569, 768
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 5 cut(s) 481, 649, 760, 772, 1153
FalI AAGNNNNNCTT 6 cut(s) 504, 536, 1203, 1235, 1279, 1311
FatI CATG 5 cut(s) 477, 645, 756, 768, 1149
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 1 cut(s) 765
FokI GGATG 1 cut(s) 579
FriOI GRGCYC 2 cut(s) 90, 810
Fsp4HI GCNGC 1 cut(s) 765
FspBI CTAG 2 cut(s) 558, 858
GlaI GCGC 1 cut(s) 262
GluI GCNGC 1 cut(s) 765
HapII CCGG 9 cut(s) 398, 424, 626, 738, 784, 953, 966, 972, 1277
HgaI GACGC 1 cut(s) 163
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 5 cut(s) 481, 649, 760, 772, 1153
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HincII GTYRAC 4 cut(s) 178, 268, 316, 963
HindII GTYRAC 4 cut(s) 178, 268, 316, 963
HindIII AAGCTT 2 cut(s) 842, 1100
HinfI GANTC 9 cut(s) 305, 458, 496, 508, 554, 575, 913, 1087, 1306
HpaI GTTAAC 1 cut(s) 963
HpaII CCGG 9 cut(s) 398, 424, 626, 738, 784, 953, 966, 972, 1277
HphI GGTGA 4 cut(s) 325, 992, 1004, 1226
Hpy166II GTNNAC 5 cut(s) 178, 268, 316, 963, 1080
Hpy188I TCNGA 4 cut(s) 597, 832, 849, 1311
Hpy8I GTNNAC 5 cut(s) 178, 268, 316, 963, 1080
Hpy99I CGWCG 5 cut(s) 317, 341, 504, 1087, 1229
HpyAV CCTTC 4 cut(s) 151, 388, 1205, 1231
HpyCH4III ACNGT 4 cut(s) 245, 337, 1160, 1442
HpyCH4IV ACGT 5 cut(s) 320, 409, 1163, 1227, 1284
HpyCH4V TGCA 3 cut(s) 1112, 1141, 1497
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 770, 939
HpyF3I CTNAG 6 cut(s) 215, 248, 669, 836, 1118, 1266
HpySE526I ACGT 5 cut(s) 320, 409, 1163, 1227, 1284
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 5 cut(s) 481, 649, 760, 772, 1153
HspAI GCGC 1 cut(s) 261
Kpn2I TCCGGA 1 cut(s) 423
KspAI GTTAAC 1 cut(s) 963
LguI GCTCTTC 1 cut(s) 1410
LmnI GCTCC 6 cut(s) 93, 224, 426, 866, 939, 1336
LweI GCATC 1 cut(s) 601
MaeI CTAG 2 cut(s) 558, 858
MaeII ACGT 5 cut(s) 320, 409, 1163, 1227, 1284
MaeIII GTNAC 8 cut(s) 146, 239, 280, 331, 355, 716, 917, 1154
MflI RGATCY 2 cut(s) 622, 652
MhlI GDGCHC 3 cut(s) 90, 810, 935
MluCI AATT 8 cut(s) 67, 119, 532, 826, 873, 904, 1027, 1192
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 321
Mph1103I ATGCAT 1 cut(s) 1143
MroI TCCGGA 1 cut(s) 423
MroXI GAANNNNTTC 2 cut(s) 110, 579
MspI CCGG 9 cut(s) 398, 424, 626, 738, 784, 953, 966, 972, 1277
MspR9I CCNGG 2 cut(s) 738, 967
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 3 cut(s) 258, 770, 939
NciI CCSGG 2 cut(s) 738, 967
NcoI CCATGG 1 cut(s) 768
NlaIII CATG 5 cut(s) 481, 649, 760, 772, 1153
NlaIV GGNNCC 2 cut(s) 624, 970
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 2 cut(s) 280, 331
NsiI ATGCAT 1 cut(s) 1143
PciSI GCTCTTC 1 cut(s) 1410
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 795
PdmI GAANNNNTTC 2 cut(s) 110, 579
PfeI GAWTC 8 cut(s) 458, 496, 508, 554, 575, 913, 1087, 1306
PflFI GACNNNGTC 1 cut(s) 173
PfoI TCCNGGA 1 cut(s) 736
PkrI GCNGC 1 cut(s) 766
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
PshAI GACNNNNGTC 1 cut(s) 1045
Psp124BI GAGCTC 2 cut(s) 90, 810
PspEI GGTNACC 1 cut(s) 239
PspN4I GGNNCC 2 cut(s) 624, 970
PspPI GGNCC 2 cut(s) 731, 969
PsuI RGATCY 2 cut(s) 622, 652
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 3 cut(s) 601, 788, 1162
RsaNI GTAC 3 cut(s) 600, 787, 1161
SacI GAGCTC 2 cut(s) 90, 810
SalI GTCGAC 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 1410
SatI GCNGC 1 cut(s) 765
Sau96I GGNCC 2 cut(s) 731, 969
ScaI AGTACT 1 cut(s) 601
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 2 cut(s) 738, 967
SduI GDGCHC 3 cut(s) 90, 810, 935
SfaNI GCATC 1 cut(s) 601
SinI GGWCC 2 cut(s) 731, 969
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 8 cut(s) 67, 119, 532, 826, 873, 904, 1027, 1192
SsiI CCGC 2 cut(s) 765, 1247
SspMI CTAG 2 cut(s) 558, 858
SstI GAGCTC 2 cut(s) 90, 810
StyD4I CCNGG 2 cut(s) 736, 965
StyI CCWWGG 2 cut(s) 569, 768
TaaI ACNGT 4 cut(s) 245, 337, 1160, 1442
TaiI ACGT 5 cut(s) 323, 412, 1166, 1230, 1287
TaqI TCGA 9 cut(s) 104, 153, 315, 606, 752, 869, 882, 1391, 1437
TaqII GACCGA 2 cut(s) 278, 1162
TasI AATT 8 cut(s) 67, 119, 532, 826, 873, 904, 1027, 1192
TatI WGTACW 1 cut(s) 599
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 8 cut(s) 458, 496, 508, 554, 575, 913, 1087, 1306
TscAI CASTG 1 cut(s) 1039
TseFI GTSAC 2 cut(s) 280, 331
Tsp45I GTSAC 2 cut(s) 280, 331
TspDTI ATGAA 3 cut(s) 17, 80, 866
TspGWI ACGGA 4 cut(s) 80, 268, 319, 827
TspRI CASTG 1 cut(s) 1039
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 2 cut(s) 731, 969
XapI RAATTY 4 cut(s) 67, 119, 532, 1027
XbaI TCTAGA 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 2 cut(s) 110, 579
XspI CTAG 2 cut(s) 558, 858
ZrmI AGTACT 1 cut(s) 601
Zsp2I ATGCAT 1 cut(s) 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.