AT4G30250

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013397
Physical Location & Seq
Reverse (-)
14810936 .. 14813107
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30250.2

Sequence Viewer

Length: 1560 bp
ATGAGTGATTATTGGACAACAATGGCTTCTCTACTTGGCATGTTAGCCTTTTGTCAAACAATCGTCCAACTCGTGTTTCCGCCTGAACTCCGACTAGCTTTCCTCCACTTCTTGACACGTATCCGCCACGTGTTCTCTTCTCACATCTACTTCGACATAACGGAGATTGACGGCGTCAACACCAACGAGCTCTACAACGCCGTTCAGCTTTACCTCAGCTCTTCCGTCACCGTCAACGACGCAGTTTCTAGCAGCAACAACAACACACGTCTCAGCTTGACACGTGTTCCTAACTCAAGCTCCGTTACTTTCGGCCTCTCCAACAACGACAGAATCACCGACGTATTTAACGGCGTCACTATCCTCTGGGAACACGTCGTTGTACAACGTCAAGTACAGAGCTTTTCGTGGAGACCAATGCCGGAAGAGAAACGAGGGTTTACGTTACAGATCAACAAAAGAGACAAAGCCCTTGTCCTAGATTCTTACCTTGATTACATTGTCGGAAAATCAGAGGAGATTCGTCGGAGAAACGAGGAGAGACTGCTTTACACGAACTCAAGAGGTGTTTCGCTCGACGCGAGGAGCCATCCATGGGATTCTGTAAGATTCAAGCATCCAAGCACGTTCGATACTCTGGCTATGGATCCTGAGAAGAAGAAGCGAATCATGGAGGATCTAAGGGAGTTTGCGAACGGACAAGGGTTTTATCAGAAAACGGGAAGAGCTTGGAAACGAGGTTACTTGTTGTATGGACCACCTGGAACTGGCAAATCGAGTTTGATTGCTGCAATGGCGAATTACCTTGGTTACGATATTTACGATCTTGAACTCACTGAGGTTCAGAACAACTCTGAATTGAGGAAGCTGTTGATGAAGACTAGCTCTAAGTCTATCATTGTCATCGAAGACATTGATTGTTCTATCAGTTTGACGAAACGAGGAAAGAACAAAAAGAAGAATGGGAGCTATGAATATGATCCAGGTTTGACAAACGGGTCGGGTTTGGAGGAACCGGGAAGCTCGGTGACTCTGTCGGGGCTATTGAACTTCACAGATGGGCTTTGGTCTTGTTGTGGGAGTGAGAAGATATTTGTGTTCACGACAAATCATATAGAGAAGTTAGATTCTGCTCTGATGAGAAGTGGGAGGATGGATATGCATGTTCACATGGGTTTTTGTAAGTTTCCGGCTTTGAAGATTCTGTTGAAGAATTATCTGAGGCTTGAGGAAGAAGATATGGATAGTGTTGTTTTGAAGGAGATGGAAGAATGTGTGGAGGAAGCAGAGATTACTCCGGCTGATGTTAGTGAAGTGTTGATTAGGAATAGGAGTGATGCGGAGAAGGCGGTGAGGGAGATTGTGAGTGTGTTGAAGGAGAGAGTTGTGAAGAGAAGGAAGAGTGTTGGATTGAAGAAGAAGAAACAGGAAGGTCAAGAAGAAGAAGAAGAAGCAGAGGAAGAACAAGAGAAGAGAGCTTTGGATAGTCCTAATAGAAGAAACAGAGAGGTTTGTGGGTTTCGAGAGGAGGAAGAAGAAGAAGATGAGAAGGAGAAGTGA

Protein Analysis

519

Amino Acids

59.51

Weight (kDa)

5.73

Isoelectric Point (pI)

58.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 28 - 128 1.2e-20 Domain associated at C-terminal with AAA
AAA PF00004 250 - 394 1.5e-17 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 396 - 464 1.7e-10 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25835 AT4G30250 AT4G30250 AT5G57480
fragaria_vesca FvH4_4g08330
malus_domestica MD13G1255400.v1.1
prunus_persica Prupe.1G086500_v2.0.a1
pyrus_communis pycom13g22720
rosa_chinensis RchiOBHm_Chr4g0402591
rosa_laevigata RLG00000009064
rosa_multiflora Rmu_co8332339.1_g000001 Rmu_sc0004921.1_g000012
rosa_roxburghii Rroxscaffold_5G00347310
rosa_rugosa Rorug04G0036900
rosa_samantha Rh4AG114500 Rh4BG106800 Rh4DG106700
rosa_wichuraiana Rw4G009130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 997
AccII CGCG 1 cut(s) 581
AciI CCGC 4 cut(s) 80, 124, 1340, 1349
AclWI GGATC 4 cut(s) 641, 654, 684, 974
AcvI CACGTG 2 cut(s) 130, 284
AcyI GRCGYC 2 cut(s) 174, 354
AfaI GTAC 2 cut(s) 384, 396
AfiI CCNNNNNNNGG 2 cut(s) 595, 767
AflIII ACRYGT 6 cut(s) 116, 129, 266, 281, 283, 373
AgsI TTSAA 8 cut(s) 613, 830, 1048, 1198, 1210, 1258, 1375, 1414
AjiI CACGTC 2 cut(s) 269, 376
AjnI CCWGG 2 cut(s) 760, 982
AjuI GAANNNNNNNTTGG 4 cut(s) 60, 92, 1463, 1495
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 4 cut(s) 275, 406, 456, 535
AlwI GGATC 4 cut(s) 641, 654, 684, 974
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 252, 788
ArsI GACNNNNNNTTYG 2 cut(s) 459, 491
AspS9I GGNCC 1 cut(s) 755
AsuC2I CCSGG 1 cut(s) 1017
AsuHPI GGTGA 4 cut(s) 220, 328, 1039, 1363
AvaII GGWCC 1 cut(s) 755
BamHI GGATCC 1 cut(s) 646
BanII GRGCYC 1 cut(s) 192
BauI CACGAG 1 cut(s) 71
BbrPI CACGTG 2 cut(s) 130, 284
BbsI GAAGAC 2 cut(s) 884, 915
Bbv12I GWGCWC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 264, 775
BccI CCATC 4 cut(s) 597, 1052, 1147, 1258
BceAI ACGGC 3 cut(s) 185, 187, 367
BciT130I CCWGG 2 cut(s) 762, 984
BciVI GTATCC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 1017
BcoDI GTCTC 4 cut(s) 275, 406, 456, 535
BfaI CTAG 4 cut(s) 95, 249, 479, 882
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 2 cut(s) 253, 789
BlsI GCNGC 2 cut(s) 254, 790
Bme1390I CCNGG 3 cut(s) 762, 984, 1017
Bme18I GGWCC 1 cut(s) 755
BmgBI CACGTC 2 cut(s) 269, 376
BmgT120I GGNCC 1 cut(s) 755
BmiI GGNNCC 3 cut(s) 587, 648, 1014
BmrFI CCNGG 3 cut(s) 762, 984, 1017
BmsI GCATC 2 cut(s) 625, 1327
BpiI GAAGAC 2 cut(s) 884, 915
Bpu10I CCTNAGC 1 cut(s) 215
BpuEI CTTGAG 3 cut(s) 280, 544, 1247
BpuMI CCSGG 1 cut(s) 1017
BsaAI YACGTR 3 cut(s) 119, 130, 284
BsaHI GRCGYC 2 cut(s) 174, 354
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 2 cut(s) 593, 805
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 2 cut(s) 595, 767
Bse1I ACTGG 1 cut(s) 772
Bse3DI GCAATG 1 cut(s) 798
BseBI CCWGG 2 cut(s) 762, 984
BseDI CCNNGG 2 cut(s) 593, 805
BseGI GGATG 3 cut(s) 589, 616, 1158
BseLI CCNNNNNNNGG 2 cut(s) 595, 767
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 5 cut(s) 229, 286, 642, 828, 1211
BseNI ACTGG 1 cut(s) 772
BseRI GAGGAG 4 cut(s) 530, 551, 598, 1541
BseXI GCAGC 2 cut(s) 264, 775
Bsh1236I CGCG 1 cut(s) 581
BshFI GGCC 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 4 cut(s) 422, 1016, 1190, 1298
BslI CCNNNNNNNGG 2 cut(s) 595, 767
BsmAI GTCTC 4 cut(s) 275, 406, 456, 535
BsmBI CGTCTC 1 cut(s) 275
BsnI GGCC 1 cut(s) 315
Bso31I GGTCTC 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 192
Bsp1407I TGTACA 1 cut(s) 382
Bsp143I GATC 5 cut(s) 450, 646, 676, 823, 979
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 4 cut(s) 80, 124, 1340, 1349
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 5 cut(s) 228, 285, 643, 829, 1212
BspFNI CGCG 1 cut(s) 581
BspLI GGNNCC 3 cut(s) 587, 648, 1014
BspPI GGATC 4 cut(s) 641, 654, 684, 974
BspQI GCTCTTC 2 cut(s) 226, 718
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 798
BsrGI TGTACA 1 cut(s) 382
BsrI ACTGG 1 cut(s) 772
BssECI CCNNGG 2 cut(s) 593, 805
BssMI GATC 5 cut(s) 450, 646, 676, 823, 979
BssNI GRCGYC 2 cut(s) 174, 354
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 2 cut(s) 593, 805
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 2 cut(s) 762, 984
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 7 cut(s) 142, 226, 420, 718, 1385, 1394, 1466
BstACI GRCGYC 2 cut(s) 174, 354
BstAUI TGTACA 1 cut(s) 382
BstBAI YACGTR 3 cut(s) 119, 130, 284
BstDEI CTNAG 7 cut(s) 215, 272, 651, 680, 837, 888, 1220
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 3 cut(s) 589, 616, 1158
BstFNI CGCG 1 cut(s) 581
BstKTI GATC 5 cut(s) 453, 649, 679, 826, 982
BstMAI GTCTC 4 cut(s) 275, 406, 456, 535
BstMBI GATC 5 cut(s) 450, 646, 676, 823, 979
BstMWI GCNNNNNNNGC 2 cut(s) 794, 1346
BstNI CCWGG 2 cut(s) 762, 984
BstNSI RCATGY 2 cut(s) 43, 1166
BstSCI CCNGG 3 cut(s) 760, 982, 1015
BstUI CGCG 1 cut(s) 581
BstV1I GCAGC 2 cut(s) 264, 775
BstV2I GAAGAC 2 cut(s) 884, 915
BstX2I RGATCY 2 cut(s) 646, 676
BstYI RGATCY 2 cut(s) 646, 676
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 593
BtrI CACGTC 2 cut(s) 269, 376
BtsCI GGATG 3 cut(s) 589, 616, 1158
BtsIMutI CAGTG 1 cut(s) 834
Cfr13I GGNCC 1 cut(s) 755
CseI GACGC 4 cut(s) 163, 248, 343, 587
Csp6I GTAC 2 cut(s) 383, 395
CviAII CATG 5 cut(s) 40, 594, 670, 1163, 1171
CviQI GTAC 2 cut(s) 383, 395
DdeI CTNAG 7 cut(s) 215, 272, 651, 680, 837, 888, 1220
DpnI GATC 5 cut(s) 452, 648, 678, 825, 981
DpnII GATC 5 cut(s) 450, 646, 676, 823, 979
DrdI GACNNNNNNGTC 1 cut(s) 997
DseDI GACNNNNNNGTC 1 cut(s) 997
Eam1104I CTCTTC 7 cut(s) 142, 226, 420, 718, 1385, 1394, 1466
EarI CTCTTC 7 cut(s) 142, 226, 420, 718, 1385, 1394, 1466
EciI GGCGGA 2 cut(s) 69, 113
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 593, 805
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 755
Eco53kI GAGCTC 1 cut(s) 190
Eco72I CACGTG 2 cut(s) 130, 284
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 760, 982
EcoT14I CCWWGG 2 cut(s) 593, 805
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 2 cut(s) 593, 805
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 5 cut(s) 43, 597, 673, 1166, 1174
FatI CATG 5 cut(s) 39, 593, 669, 1162, 1170
Fnu4HI GCNGC 2 cut(s) 253, 789
FokI GGATG 3 cut(s) 576, 603, 1165
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 2 cut(s) 253, 789
FspBI CTAG 4 cut(s) 95, 249, 479, 882
GluI GCNGC 2 cut(s) 253, 789
HaeIII GGCC 1 cut(s) 315
HapII CCGG 4 cut(s) 422, 1016, 1190, 1298
HgaI GACGC 4 cut(s) 163, 248, 343, 587
Hin1I GRCGYC 2 cut(s) 174, 354
Hin1II CATG 5 cut(s) 43, 597, 673, 1166, 1174
HincII GTYRAC 2 cut(s) 178, 235
HindII GTYRAC 2 cut(s) 178, 235
HinfI GANTC 9 cut(s) 333, 482, 520, 599, 609, 666, 1030, 1127, 1201
HpaII CCGG 4 cut(s) 422, 1016, 1190, 1298
HphI GGTGA 4 cut(s) 220, 328, 1039, 1363
Hpy166II GTNNAC 5 cut(s) 178, 235, 441, 1101, 1168
Hpy188I TCNGA 9 cut(s) 92, 506, 514, 528, 714, 846, 856, 1137, 1221
Hpy188III TCNNGA 7 cut(s) 112, 561, 650, 827, 1102, 1436, 1523
Hpy8I GTNNAC 5 cut(s) 178, 235, 441, 1101, 1168
Hpy99I CGWCG 5 cut(s) 242, 344, 380, 528, 581
HpyAV CCTTC 6 cut(s) 1252, 1339, 1369, 1389, 1424, 1543
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 9 cut(s) 118, 129, 268, 283, 342, 375, 388, 443, 626
HpyCH4V TGCA 2 cut(s) 791, 1162
HpyF10VI GCNNNNNNNGC 2 cut(s) 794, 1346
HpyF3I CTNAG 7 cut(s) 215, 272, 651, 680, 837, 888, 1220
HpySE526I ACGT 9 cut(s) 118, 129, 268, 283, 342, 375, 388, 443, 626
Hsp92I GRCGYC 2 cut(s) 174, 354
Hsp92II CATG 5 cut(s) 43, 597, 673, 1166, 1174
Kzo9I GATC 5 cut(s) 450, 646, 676, 823, 979
LguI GCTCTTC 2 cut(s) 226, 718
LmnI GCTCC 3 cut(s) 305, 585, 966
Lsp1109I GCAGC 2 cut(s) 264, 775
LweI GCATC 2 cut(s) 625, 1327
MaeI CTAG 4 cut(s) 95, 249, 479, 882
MaeII ACGT 9 cut(s) 118, 129, 268, 283, 342, 375, 388, 443, 626
MaeIII GTNAC 7 cut(s) 226, 304, 355, 444, 740, 809, 1027
MalI GATC 5 cut(s) 452, 648, 678, 825, 981
MboI GATC 5 cut(s) 450, 646, 676, 823, 979
MflI RGATCY 2 cut(s) 646, 676
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 3 cut(s) 799, 857, 1213
MlyI GAGTC 1 cut(s) 1024
MmeI TCCRAC 6 cut(s) 91, 115, 345, 484, 506, 1387
Mph1103I ATGCAT 1 cut(s) 1164
MseI TTAA 1 cut(s) 348
MspI CCGG 4 cut(s) 422, 1016, 1190, 1298
MspR9I CCNGG 3 cut(s) 762, 984, 1017
MvaI CCWGG 2 cut(s) 762, 984
MvnI CGCG 1 cut(s) 581
MwoI GCNNNNNNNGC 2 cut(s) 794, 1346
NciI CCSGG 1 cut(s) 1017
NcoI CCATGG 1 cut(s) 593
NdeII GATC 5 cut(s) 450, 646, 676, 823, 979
NlaIII CATG 5 cut(s) 43, 597, 673, 1166, 1174
NlaIV GGNNCC 3 cut(s) 587, 648, 1014
NmuCI GTSAC 3 cut(s) 226, 355, 1027
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 2 cut(s) 43, 1166
PciSI GCTCTTC 2 cut(s) 226, 718
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 578, 819
PfeI GAWTC 8 cut(s) 333, 482, 520, 599, 609, 666, 1127, 1201
PflFI GACNNNGTC 2 cut(s) 173, 1033
PkrI GCNGC 2 cut(s) 254, 790
PleI GAGTC 1 cut(s) 1024
PmaCI CACGTG 2 cut(s) 130, 284
PmlI CACGTG 2 cut(s) 130, 284
PpsI GAGTC 1 cut(s) 1024
Ppu21I YACGTR 3 cut(s) 119, 130, 284
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 760, 982
PspCI CACGTG 2 cut(s) 130, 284
PspGI CCWGG 2 cut(s) 760, 982
PspN4I GGNNCC 3 cut(s) 587, 648, 1014
PspPI GGNCC 1 cut(s) 755
PsuI RGATCY 2 cut(s) 646, 676
PsyI GACNNNGTC 2 cut(s) 173, 1033
RsaI GTAC 2 cut(s) 384, 396
RsaNI GTAC 2 cut(s) 383, 395
SacI GAGCTC 1 cut(s) 192
SapI GCTCTTC 2 cut(s) 226, 718
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 2 cut(s) 253, 789
Sau3AI GATC 5 cut(s) 450, 646, 676, 823, 979
Sau96I GGNCC 1 cut(s) 755
SchI GAGTC 1 cut(s) 1024
ScrFI CCNGG 3 cut(s) 762, 984, 1017
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 2 cut(s) 625, 1327
SinI GGWCC 1 cut(s) 755
SmlI CTYRAG 3 cut(s) 295, 559, 1226
SmoI CTYRAG 3 cut(s) 295, 559, 1226
Sse9I AATT 3 cut(s) 799, 857, 1213
SsiI CCGC 4 cut(s) 80, 124, 1340, 1349
SspMI CTAG 4 cut(s) 95, 249, 479, 882
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 3 cut(s) 760, 982, 1015
StyI CCWWGG 2 cut(s) 593, 805
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 9 cut(s) 121, 132, 271, 286, 345, 378, 391, 446, 629
TaqI TCGA 6 cut(s) 153, 576, 630, 776, 906, 1522
TasI AATT 3 cut(s) 799, 857, 1213
TatI WGTACW 2 cut(s) 382, 394
TfiI GAWTC 8 cut(s) 333, 482, 520, 599, 609, 666, 1127, 1201
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 841
TseFI GTSAC 3 cut(s) 226, 355, 1027
TseI GCWGC 2 cut(s) 252, 788
Tsp45I GTSAC 3 cut(s) 226, 355, 1027
TspDTI ATGAA 2 cut(s) 890, 987
TspGWI ACGGA 4 cut(s) 176, 214, 292, 711
TspRI CASTG 1 cut(s) 841
Tth111I GACNNNGTC 2 cut(s) 173, 1033
VpaK11BI GGWCC 1 cut(s) 755
XceI RCATGY 2 cut(s) 43, 1166
XspI CTAG 4 cut(s) 95, 249, 479, 882
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.