Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0013397

Physical Location & Seq

Genomic Coordinates Reverse (-)

27903749 .. 27905329

Gene Structure 1581 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1255400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGAAGGAGTATTGGACCTCTTTGGCTTCTTTGCTAGGCGTTTTGGCCTTCTGCCAGAGCCTCCTCCAAGCAGTGTTCCCACCGGAGCTCCGCTACGCCTTCGCCAAGCTCTTCAACAAGATCTTCCACTGGTTCTCCTCCTACTACTACTTCGACATCACGGAGATCGATGGCGTCAACACCAACGAGCTCTACAACGCCGTGCAGCTCTACCTCAGCTCCACGGTCTCCATCACTGGCACCCGCCTCAGCCTCACGCGCGCCCTCAACTCCAGCGCCATCACCTTCGGCCTCTCCAACAACGACTGCATGGTGGACAACTTCAACGGCGTTACCGTCCTCTGGGAGCACGTTGTCACGCAACGGCAGAACCAGACCTTCTCCTGGCGGCCGCTGCCGGAGGAGAAGCGAGGATTCACCCTCCGCATCAAGAAGAAGGACAAGTTACTCATCCTCAACAACTACTTGGACTATATCATGGAAAAGGCCAATGAAATCCGGAGAAAAAACCAGGATCGGCTTCTGTACACGAATTCCCGTGGCGGATCGTTGGATTCCCGCGGACATCCTTGGGAATCCGTGCCGTTCAAGCATCCGAGCACGTTCGAGACGCTGGCGATGGACCCGAAGACGAAGAGGGAGATCATGGACGATCTCATGGATTTCGCCAATGGCCAAAACTTTTACCAGAAAACAGGGAGAGCTTGGAAGAGGGGTTACCTTTTGTACGGACCTCCGGGGACCGGAAAATCGAGCATGATCGCTGCAATGGCGAATTATCTTGGTTACGATATTTATGACCTTGAACTCACGGAGGTGCACACCAACTCCGAGCTGCGGAAGTTGCTGATGAAGACTAGTTCGAAGTCGATTATTGTGATCGAAGATATTGATTGTTCGATCAATCTGACGAACAGGAAGAACATTTGTGGCGGAGGGAATGCGGGTAGGAATTACTATGAATCCGGGGAGATAAGAGGTGGTGTTGGTGGTTCTGGGGAGGAGGGTGGCGGAGGAAATTCGATTACGCTTTCGGGGTTGTTGAATTTCACTGATGGGTTGTGGTCGTGCTGTGGGAGTGAGAGAATTTTCGTGTTCACAACCAACCACATTGAGAAGCTTGATCCTGCATTGCTGAGGAGTGGGAGAATGGATATGCATATTTTCATGAGCCACTGTTCGTTCCCGGCGTTGAAGATTCTGCTGAGGAATTATTTGGGGTACGACGAGGGGGATTTGGATGAGGAGATTACAAGGGAGTTTGAAGAGGTTTTGGAGAAGGCGGCAATGACTCCTGCGGATGTGAGTGAGGTGTTGATTAAGAACAGGAGGGATAAGGGCAATGCGGTGATGGAATTGCTGGAGGCCTTGAAGGCGAGGGCGGAGAGGAGGAAGAAGAAAGAGAGTAATGGGGGTGGTGAGTTGGAAGAGGAAGAAGATGTAGAAGAGGAAGAAGAGCAGGAGAAGAGGGCATTGGAGAGTCCTAAAGATGAAGGGTCATGTGACCTTGTTCAGGACAGCTGCAAGAAAGGTGATGAAGAGGATGATCAAAAACAAGATGATGACATTATAGAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006810 GO:0008104 GO:0008150 GO:0008320 GO:0008565 GO:0009987 GO:0015031 GO:0015833 GO:0016043 GO:0022607 GO:0022857 GO:0022884 GO:0033036 GO:0034622 GO:0042886 GO:0042887 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0051131 GO:0051179 GO:0051234 GO:0055085 GO:0065003 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:1904680

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA AAA_assoc

Protein Analysis

527

Amino Acids

59.7

Weight (kDa)

5.1

Isoelectric Point (pI)

51.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_assoc	PF14363	26 - 118	8.8e-22	Domain associated at C-terminal with AAA
AAA	PF00004	240 - 301	1.1e-10	ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540	PF25568	393 - 468	1e-11	At3g28540-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013397)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25835 AT4G30250 AT4G30250 AT5G57480
fragaria_vesca	FvH4_4g08330
malus_domestica	MD13G1255400.v1.1
prunus_persica	Prupe.1G086500_v2.0.a1
pyrus_communis	pycom13g22720
rosa_chinensis	RchiOBHm_Chr4g0402591
rosa_laevigata	RLG00000009064
rosa_multiflora	Rmu_co8332339.1_g000001 Rmu_sc0004921.1_g000012
rosa_roxburghii	Rroxscaffold_5G00347310
rosa_rugosa	Rorug04G0036900
rosa_samantha	Rh4AG114500 Rh4BG106800 Rh4DG106700
rosa_wichuraiana	Rw4G009130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	239
AccII	CGCG	3 cut(s)	259, 261, 561
AccIII	TCCGGA	1 cut(s)	498
AclWI	GGATC	3 cut(s)	522, 553, 1118
AcoI	YGGCCR	2 cut(s)	389, 673
AcsI	RAATTY	4 cut(s)	532, 1018, 1045, 1086
AcyI	GRCGYC	1 cut(s)	174
AfaI	GTAC	3 cut(s)	527, 728, 1223
AfiI	CCNNNNNNNGG	5 cut(s)	342, 384, 743, 837, 1513
AgsI	TTSAA	8 cut(s)	115, 325, 589, 806, 1045, 1195, 1265, 1372
AhlI	ACTAGT	1 cut(s)	857
AjnI	CCWGG	2 cut(s)	383, 510
AjuI	GAANNNNNNNTTGG	2 cut(s)	1457, 1489
AleI	CACNNNNGTG	1 cut(s)	815
AluBI	AGCT	9 cut(s)	88, 109, 190, 208, 219, 704, 835, 1120, 1521
AluI	AGCT	9 cut(s)	88, 109, 190, 208, 219, 704, 835, 1120, 1521
Alw21I	GWGCWC	5 cut(s)	90, 192, 351, 602, 822
Alw26I	GTCTC	2 cut(s)	232, 602
Alw44I	GTGCAC	1 cut(s)	818
AlwI	GGATC	3 cut(s)	522, 553, 1118
Aor13HI	TCCGGA	1 cut(s)	498
AoxI	GGCC	6 cut(s)	45, 289, 389, 486, 673, 1365
ApaLI	GTGCAC	1 cut(s)	818
ApeKI	GCWGC	5 cut(s)	205, 394, 764, 835, 1521
ApoI	RAATTY	4 cut(s)	532, 1018, 1045, 1086
AspLEI	GCGC	3 cut(s)	261, 263, 278
AspS9I	GGNCC	4 cut(s)	15, 622, 731, 741
AsuC2I	CCSGG	3 cut(s)	738, 967, 1187
AsuHPI	GGTGA	5 cut(s)	274, 409, 1360, 1430, 1544
AsuII	TTCGAA	1 cut(s)	863
AvaII	GGWCC	4 cut(s)	15, 622, 731, 741
BaeGI	GKGCMC	1 cut(s)	822
BalI	TGGCCA	1 cut(s)	675
BanI	GGYRCC	1 cut(s)	239
BanII	GRGCYC	2 cut(s)	90, 192
BarI	GAAGNNNNNNTAC	2 cut(s)	701, 733
BbsI	GAAGAC	2 cut(s)	635, 860
Bbv12I	GWGCWC	5 cut(s)	90, 192, 351, 602, 822
BbvCI	CCTCAGC	4 cut(s)	215, 248, 1136, 1205
BbvI	GCAGC	5 cut(s)	217, 381, 751, 822, 1508
BccI	CCATC	6 cut(s)	164, 239, 287, 613, 1049, 1345
BceAI	ACGGC	4 cut(s)	185, 343, 380, 568
BciT130I	CCWGG	2 cut(s)	385, 512
BclI	TGATCA	1 cut(s)	1546
BcnI	CCSGG	3 cut(s)	738, 967, 1187
BcoDI	GTCTC	2 cut(s)	232, 602
BcuI	ACTAGT	1 cut(s)	857
BfaI	CTAG	2 cut(s)	35, 858
BfoI	RGCGCY	1 cut(s)	279
BglI	GCCNNNNNGGC	1 cut(s)	1373
BglII	AGATCT	1 cut(s)	120
BisI	GCNGC	8 cut(s)	206, 389, 392, 395, 765, 836, 1284, 1522
BlsI	GCNGC	8 cut(s)	207, 390, 393, 396, 766, 837, 1285, 1523
Bme1390I	CCNGG	5 cut(s)	385, 512, 738, 967, 1187
Bme18I	GGWCC	4 cut(s)	15, 622, 731, 741
BmgT120I	GGNCC	4 cut(s)	15, 622, 731, 741
BmiI	GGNNCC	3 cut(s)	241, 624, 742
BmrFI	CCNGG	5 cut(s)	385, 512, 738, 967, 1187
BmsI	GCATC	2 cut(s)	435, 601
BpiI	GAAGAC	2 cut(s)	635, 860
BpmI	CTGGAG	2 cut(s)	256, 1382
Bpu10I	CCTNAGC	4 cut(s)	215, 248, 1136, 1205
Bpu14I	TTCGAA	1 cut(s)	863
BpuMI	CCSGG	3 cut(s)	738, 967, 1187
Bsa29I	ATCGAT	1 cut(s)	168
BsaHI	GRCGYC	1 cut(s)	174
BsaI	GGTCTC	1 cut(s)	232
BsaJI	CCNNGG	6 cut(s)	222, 538, 559, 569, 737, 966
BsaWI	WCCGGW	3 cut(s)	82, 498, 743
BsaXI	ACNNNNNCTCC	8 cut(s)	72, 102, 119, 149, 203, 233, 812, 842
Bsc4I	CCNNNNNNNGG	5 cut(s)	342, 384, 743, 837, 1513
Bse1I	ACTGG	2 cut(s)	134, 241
Bse3DI	GCAATG	4 cut(s)	774, 1130, 1293, 1348
BseAI	TCCGGA	1 cut(s)	498
BseBI	CCWGG	2 cut(s)	385, 512
BseCI	ATCGAT	1 cut(s)	168
BseDI	CCNNGG	6 cut(s)	222, 538, 559, 569, 737, 966
BseGI	GGATG	6 cut(s)	450, 565, 592, 1246, 1306, 1549
BseLI	CCNNNNNNNGG	5 cut(s)	342, 384, 743, 837, 1513
BseMI	GCAATG	4 cut(s)	774, 1130, 1293, 1348
BseMII	CTCAG	4 cut(s)	229, 262, 1127, 1196
BseNI	ACTGG	2 cut(s)	134, 241
BsePI	GCGCGC	1 cut(s)	259
BseRI	GAGGAG	7 cut(s)	53, 127, 416, 1016, 1153, 1259, 1402
BseSI	GKGCMC	1 cut(s)	822
BseX3I	CGGCCG	1 cut(s)	389
BseXI	GCAGC	5 cut(s)	217, 381, 751, 822, 1508
BsgI	GTGCAG	1 cut(s)	224
Bsh1236I	CGCG	3 cut(s)	259, 261, 561
Bsh1285I	CGRYCG	1 cut(s)	392
BshFI	GGCC	6 cut(s)	47, 291, 391, 488, 675, 1367
BshNI	GGYRCC	1 cut(s)	239
BshVI	ATCGAT	1 cut(s)	168
BsiEI	CGRYCG	1 cut(s)	392
BsiHKAI	GWGCWC	5 cut(s)	90, 192, 351, 602, 822
BsiSI	CCGG	7 cut(s)	83, 398, 499, 737, 744, 966, 1187
BslFI	GGGAC	1 cut(s)	754
BslI	CCNNNNNNNGG	5 cut(s)	342, 384, 743, 837, 1513
BsmAI	GTCTC	2 cut(s)	232, 602
BsmBI	CGTCTC	1 cut(s)	602
BsmFI	GGGAC	1 cut(s)	754
BsmI	GAATGC	1 cut(s)	946
BsnI	GGCC	6 cut(s)	47, 291, 391, 488, 675, 1367
Bso31I	GGTCTC	1 cut(s)	232
Bsp119I	TTCGAA	1 cut(s)	863
Bsp1286I	GDGCHC	5 cut(s)	90, 192, 351, 602, 822
Bsp13I	TCCGGA	1 cut(s)	498
Bsp1407I	TGTACA	1 cut(s)	525
BspANI	GGCC	6 cut(s)	47, 291, 391, 488, 675, 1367
BspCNI	CTCAG	4 cut(s)	228, 261, 1128, 1197
BspDI	ATCGAT	1 cut(s)	168
BspEI	TCCGGA	1 cut(s)	498
BspFNI	CGCG	3 cut(s)	259, 261, 561
BspHI	TCATGA	1 cut(s)	1167
BspLI	GGNNCC	3 cut(s)	241, 624, 742
BspPI	GGATC	3 cut(s)	522, 553, 1118
BspQI	GCTCTTC	2 cut(s)	116, 1449
BspT104I	TTCGAA	1 cut(s)	863
BspT107I	GGYRCC	1 cut(s)	239
BspTNI	GGTCTC	1 cut(s)	232
BsrDI	GCAATG	4 cut(s)	774, 1130, 1293, 1348
BsrGI	TGTACA	1 cut(s)	525
BsrI	ACTGG	2 cut(s)	134, 241
BssECI	CCNNGG	6 cut(s)	222, 538, 559, 569, 737, 966
BssHII	GCGCGC	1 cut(s)	259
BssNI	GRCGYC	1 cut(s)	174
BssT1I	CCWWGG	1 cut(s)	569
Bst2UI	CCWGG	2 cut(s)	385, 512
Bst4CI	ACNGT	3 cut(s)	226, 337, 1178
Bst6I	CTCTTC	9 cut(s)	116, 629, 704, 1260, 1422, 1440, 1449, 1460, 1533
BstACI	GRCGYC	1 cut(s)	174
BstAUI	TGTACA	1 cut(s)	525
BstBI	TTCGAA	1 cut(s)	863
BstC8I	GCNNGC	2 cut(s)	261, 615
BstDEI	CTNAG	4 cut(s)	215, 248, 1136, 1205
BstDSI	CCRYGG	3 cut(s)	222, 538, 559
BstEII	GGTNACC	1 cut(s)	716
BstENI	CCTNNNNNAGG	1 cut(s)	1511
BstF5I	GGATG	6 cut(s)	450, 565, 592, 1246, 1306, 1549
BstFNI	CGCG	3 cut(s)	259, 261, 561
BstH2I	RGCGCY	1 cut(s)	279
BstHHI	GCGC	3 cut(s)	261, 263, 278
BstMAI	GTCTC	2 cut(s)	232, 602
BstMCI	CGRYCG	1 cut(s)	392
BstMWI	GCNNNNNNNGC	6 cut(s)	258, 394, 589, 770, 844, 1373
BstNI	CCWGG	2 cut(s)	385, 512
BstPI	GGTNACC	1 cut(s)	716
BstSCI	CCNGG	5 cut(s)	383, 510, 736, 965, 1185
BstSLI	GKGCMC	1 cut(s)	822
BstUI	CGCG	3 cut(s)	259, 261, 561
BstV1I	GCAGC	5 cut(s)	217, 381, 751, 822, 1508
BstV2I	GAAGAC	2 cut(s)	635, 860
BstX2I	RGATCY	1 cut(s)	120
BstYI	RGATCY	1 cut(s)	120
BstZI	CGGCCG	1 cut(s)	389
Bsu15I	ATCGAT	1 cut(s)	168
BsuRI	GGCC	6 cut(s)	47, 291, 391, 488, 675, 1367
BsuTUI	ATCGAT	1 cut(s)	168
BtgI	CCRYGG	3 cut(s)	222, 538, 559
BtgZI	GCGATG	1 cut(s)	632
BtsCI	GGATG	6 cut(s)	450, 565, 592, 1246, 1306, 1549
BtsI	GCAGTG	1 cut(s)	78
BtsIMutI	CAGTG	5 cut(s)	78, 127, 234, 1050, 1174
Cac8I	GCNNGC	2 cut(s)	261, 615
CciI	TCATGA	1 cut(s)	1167
CciNI	GCGGCCGC	1 cut(s)	389
CfoI	GCGC	3 cut(s)	261, 263, 278
Cfr13I	GGNCC	4 cut(s)	15, 622, 731, 741
Cfr42I	CCGCGG	1 cut(s)	562
ClaI	ATCGAT	1 cut(s)	168
CseI	GACGC	2 cut(s)	163, 619
Csp6I	GTAC	3 cut(s)	526, 727, 1222
CviAII	CATG	7 cut(s)	310, 478, 646, 658, 757, 1168, 1500
CviQI	GTAC	3 cut(s)	526, 727, 1222
DdeI	CTNAG	4 cut(s)	215, 248, 1136, 1205
EaeI	YGGCCR	2 cut(s)	389, 673
EagI	CGGCCG	1 cut(s)	389
Eam1104I	CTCTTC	9 cut(s)	116, 629, 704, 1260, 1422, 1440, 1449, 1460, 1533
EarI	CTCTTC	9 cut(s)	116, 629, 704, 1260, 1422, 1440, 1449, 1460, 1533
EciI	GGCGGA	4 cut(s)	558, 948, 1026, 1397
Ecl136II	GAGCTC	2 cut(s)	88, 190
EclXI	CGGCCG	1 cut(s)	389
Eco130I	CCWWGG	1 cut(s)	569
Eco147I	AGGCCT	1 cut(s)	1367
Eco24I	GRGCYC	2 cut(s)	90, 192
Eco31I	GGTCTC	1 cut(s)	232
Eco47I	GGWCC	4 cut(s)	15, 622, 731, 741
Eco52I	CGGCCG	1 cut(s)	389
Eco53kI	GAGCTC	2 cut(s)	88, 190
Eco91I	GGTNACC	1 cut(s)	716
EcoICRI	GAGCTC	2 cut(s)	88, 190
EcoNI	CCTNNNNNAGG	1 cut(s)	1511
EcoO65I	GGTNACC	1 cut(s)	716
EcoRI	GAATTC	1 cut(s)	532
EcoRII	CCWGG	2 cut(s)	383, 510
EcoT14I	CCWWGG	1 cut(s)	569
EcoT22I	ATGCAT	1 cut(s)	1161
EcoT38I	GRGCYC	2 cut(s)	90, 192
ErhI	CCWWGG	1 cut(s)	569
Esp3I	CGTCTC	1 cut(s)	602
FaeI	CATG	7 cut(s)	313, 481, 649, 661, 760, 1171, 1503
FaqI	GGGAC	1 cut(s)	754
FatI	CATG	7 cut(s)	309, 477, 645, 657, 756, 1167, 1499
FauI	CCCGC	3 cut(s)	251, 566, 937
FbaI	TGATCA	1 cut(s)	1546
Fnu4HI	GCNGC	8 cut(s)	206, 389, 392, 395, 765, 836, 1284, 1522
FokI	GGATG	6 cut(s)	437, 552, 579, 1253, 1313, 1556
FriOI	GRGCYC	2 cut(s)	90, 192
Fsp4HI	GCNGC	8 cut(s)	206, 389, 392, 395, 765, 836, 1284, 1522
FspBI	CTAG	2 cut(s)	35, 858
GlaI	GCGC	3 cut(s)	260, 262, 277
GluI	GCNGC	8 cut(s)	206, 389, 392, 395, 765, 836, 1284, 1522
GsuI	CTGGAG	2 cut(s)	256, 1382
HaeII	RGCGCY	1 cut(s)	279
HaeIII	GGCC	6 cut(s)	47, 291, 391, 488, 675, 1367
HapII	CCGG	7 cut(s)	83, 398, 499, 737, 744, 966, 1187
HgaI	GACGC	2 cut(s)	163, 619
HhaI	GCGC	3 cut(s)	261, 263, 278
Hin1I	GRCGYC	1 cut(s)	174
Hin1II	CATG	7 cut(s)	313, 481, 649, 661, 760, 1171, 1503
Hin6I	GCGC	3 cut(s)	259, 261, 276
HinP1I	GCGC	3 cut(s)	259, 261, 276
HincII	GTYRAC	1 cut(s)	178
HindII	GTYRAC	1 cut(s)	178
HindIII	AAGCTT	1 cut(s)	1118
HinfI	GANTC	7 cut(s)	414, 554, 575, 962, 1198, 1291, 1480
HpaII	CCGG	7 cut(s)	83, 398, 499, 737, 744, 966, 1187
HphI	GGTGA	5 cut(s)	274, 409, 1360, 1430, 1544
Hpy166II	GTNNAC	5 cut(s)	178, 316, 528, 820, 1098
Hpy188I	TCNGA	3 cut(s)	597, 832, 909
Hpy188III	TCNNGA	5 cut(s)	430, 499, 607, 1168, 1514
Hpy8I	GTNNAC	5 cut(s)	178, 316, 528, 820, 1098
Hpy99I	CGWCG	1 cut(s)	1229
HpyAV	CCTTC	8 cut(s)	58, 109, 295, 388, 430, 1273, 1366, 1487
HpyCH4III	ACNGT	3 cut(s)	226, 337, 1178
HpyCH4IV	ACGT	2 cut(s)	351, 602
HpyCH4V	TGCA	7 cut(s)	205, 309, 767, 820, 1130, 1159, 1524
HpyF10VI	GCNNNNNNNGC	6 cut(s)	258, 394, 589, 770, 844, 1373
HpyF3I	CTNAG	4 cut(s)	215, 248, 1136, 1205
HpySE526I	ACGT	2 cut(s)	351, 602
Hsp92I	GRCGYC	1 cut(s)	174
Hsp92II	CATG	7 cut(s)	313, 481, 649, 661, 760, 1171, 1503
HspAI	GCGC	3 cut(s)	259, 261, 276
Kpn2I	TCCGGA	1 cut(s)	498
Ksp22I	TGATCA	1 cut(s)	1546
KspI	CCGCGG	1 cut(s)	562
LguI	GCTCTTC	2 cut(s)	116, 1449
LmnI	GCTCC	4 cut(s)	85, 93, 224, 346
Lsp1109I	GCAGC	5 cut(s)	217, 381, 751, 822, 1508
LweI	GCATC	2 cut(s)	435, 601
MaeI	CTAG	2 cut(s)	35, 858
MaeII	ACGT	2 cut(s)	351, 602
MaeIII	GTNAC	6 cut(s)	331, 355, 444, 716, 785, 1502
MflI	RGATCY	1 cut(s)	120
MhlI	GDGCHC	5 cut(s)	90, 192, 351, 602, 822
MlsI	TGGCCA	1 cut(s)	675
MluCI	AATT	8 cut(s)	532, 775, 952, 1018, 1045, 1086, 1210, 1355
MluNI	TGGCCA	1 cut(s)	675
MlyI	GAGTC	2 cut(s)	1285, 1489
MmeI	TCCRAC	3 cut(s)	321, 531, 1404
Mox20I	TGGCCA	1 cut(s)	675
Mph1103I	ATGCAT	1 cut(s)	1161
MroI	TCCGGA	1 cut(s)	498
MscI	TGGCCA	1 cut(s)	675
MseI	TTAA	1 cut(s)	1320
MslI	CAYNNNNRTG	1 cut(s)	815
Msp20I	TGGCCA	1 cut(s)	675
MspA1I	CMGCKG	3 cut(s)	394, 561, 1521
MspI	CCGG	7 cut(s)	83, 398, 499, 737, 744, 966, 1187
MspR9I	CCNGG	5 cut(s)	385, 512, 738, 967, 1187
Mva1269I	GAATGC	1 cut(s)	946
MvaI	CCWGG	2 cut(s)	385, 512
MvnI	CGCG	3 cut(s)	259, 261, 561
MwoI	GCNNNNNNNGC	6 cut(s)	258, 394, 589, 770, 844, 1373
NciI	CCSGG	3 cut(s)	738, 967, 1187
NlaIII	CATG	7 cut(s)	313, 481, 649, 661, 760, 1171, 1503
NlaIV	GGNNCC	3 cut(s)	241, 624, 742
NmuCI	GTSAC	2 cut(s)	355, 1502
NotI	GCGGCCGC	1 cut(s)	389
NsiI	ATGCAT	1 cut(s)	1161
NspV	TTCGAA	1 cut(s)	863
OliI	CACNNNNGTG	1 cut(s)	815
PagI	TCATGA	1 cut(s)	1167
PauI	GCGCGC	1 cut(s)	259
PceI	AGGCCT	1 cut(s)	1367
PciSI	GCTCTTC	2 cut(s)	116, 1449
PcsI	WCGNNNNNNNCGW	2 cut(s)	333, 1187
PctI	GAATGC	1 cut(s)	946
PfeI	GAWTC	5 cut(s)	414, 554, 575, 962, 1198
PkrI	GCNGC	8 cut(s)	207, 390, 393, 396, 766, 837, 1285, 1523
PleI	GAGTC	2 cut(s)	1285, 1488
PpsI	GAGTC	2 cut(s)	1285, 1488
Psp124BI	GAGCTC	2 cut(s)	90, 192
Psp6I	CCWGG	2 cut(s)	383, 510
PspEI	GGTNACC	1 cut(s)	716
PspGI	CCWGG	2 cut(s)	383, 510
PspN4I	GGNNCC	3 cut(s)	241, 624, 742
PspPI	GGNCC	4 cut(s)	15, 622, 731, 741
PsuI	RGATCY	1 cut(s)	120
PteI	GCGCGC	1 cut(s)	259
PvuII	CAGCTG	1 cut(s)	1521
RsaI	GTAC	3 cut(s)	527, 728, 1223
RsaNI	GTAC	3 cut(s)	526, 727, 1222
RseI	CAYNNNNRTG	1 cut(s)	815
SacI	GAGCTC	2 cut(s)	90, 192
SacII	CCGCGG	1 cut(s)	562
SapI	GCTCTTC	2 cut(s)	116, 1449
SaqAI	TTAA	1 cut(s)	1320
SatI	GCNGC	8 cut(s)	206, 389, 392, 395, 765, 836, 1284, 1522
Sau96I	GGNCC	4 cut(s)	15, 622, 731, 741
SchI	GAGTC	2 cut(s)	1285, 1489
ScrFI	CCNGG	5 cut(s)	385, 512, 738, 967, 1187
SduI	GDGCHC	5 cut(s)	90, 192, 351, 602, 822
SfaNI	GCATC	2 cut(s)	435, 601
Sfr303I	CCGCGG	1 cut(s)	562
SfuI	TTCGAA	1 cut(s)	863
SgrBI	CCGCGG	1 cut(s)	562
SinI	GGWCC	4 cut(s)	15, 622, 731, 741
SmiMI	CAYNNNNRTG	1 cut(s)	815
SpeI	ACTAGT	1 cut(s)	857
Sse9I	AATT	8 cut(s)	532, 775, 952, 1018, 1045, 1086, 1210, 1355
SseBI	AGGCCT	1 cut(s)	1367
SspMI	CTAG	2 cut(s)	35, 858
SstI	GAGCTC	2 cut(s)	90, 192
StuI	AGGCCT	1 cut(s)	1367
StyD4I	CCNGG	5 cut(s)	383, 510, 736, 965, 1185
StyI	CCWWGG	1 cut(s)	569
TaaI	ACNGT	3 cut(s)	226, 337, 1178
TaiI	ACGT	2 cut(s)	354, 605
TaqI	TCGA	9 cut(s)	153, 168, 606, 752, 863, 869, 882, 899, 1022
TasI	AATT	8 cut(s)	532, 775, 952, 1018, 1045, 1086, 1210, 1355
TatI	WGTACW	1 cut(s)	525
TauI	GCSGC	3 cut(s)	391, 394, 1286
TfiI	GAWTC	5 cut(s)	414, 554, 575, 962, 1198
Tru1I	TTAA	1 cut(s)	1320
Tru9I	TTAA	1 cut(s)	1320
TscAI	CASTG	5 cut(s)	78, 134, 241, 1057, 1181
TseFI	GTSAC	2 cut(s)	355, 1502
TseI	GCWGC	5 cut(s)	205, 394, 764, 835, 1521
Tsp45I	GTSAC	2 cut(s)	355, 1502
TspDTI	ATGAA	7 cut(s)	17, 507, 866, 975, 1156, 1506, 1551
TspGWI	ACGGA	4 cut(s)	176, 568, 744, 827
TspRI	CASTG	5 cut(s)	78, 134, 241, 1057, 1181
VneI	GTGCAC	1 cut(s)	818
VpaK11BI	GGWCC	4 cut(s)	15, 622, 731, 741
XagI	CCTNNNNNAGG	1 cut(s)	1511
XapI	RAATTY	4 cut(s)	532, 1018, 1045, 1086
XspI	CTAG	2 cut(s)	35, 858
Zsp2I	ATGCAT	1 cut(s)	1161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.