Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007034

Physical Location & Seq

Genomic Coordinates Forward (+)

13154708 .. 13159382

Gene Structure 4675 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G25880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGCAACTGAGAATCCTATTAGGATATCTGGAAGCAATGAAAGATGGTCTAATTCTAGGAAGGTTTCTGTTCCTAATCGAAGTGGCAGTGCGCCACCGAACATGGAGGGGTCTTTTCTAGCTGTGGATAATCTATTGTCACGACAGGGCGGCTCAGTTTACAATAACTTGATGCTACCGCGTTACGGGTTTGAGGAGCCCGTGACCACTCATCCTTCTTCTAAACATAGCCTTAACCGCATACCTTCTCCACCGATTTATTACCCTACTGAGTATCAGTTCATAGACAATCGTGTAGGTAGATTTAGAAGTAACCAGGGATTGAATAAAGTTAATTCTCCCATTCATTTGTCCCAAGGCAAACTTTCTACTCATAAGGAAGTTTCTGAGGATGAAAGCTCCCAACAGTTATCTGTTAATAGTGTTTCAGATAGGACAGATGGATTGGACATTCGTCTTTCACCTGGTTCTCAAAGTTTGGCCGATTTCAGGCAGGATGATACTTCTTCTGGGCAGACCCCTCAACATAGTCGGTCTAACTCTTCAAATGGTGAAGTGAACACAGCAGATGAGAGTGGTAACTTCTCAGAGTTGTCAGATGATGTCGTGGTTAAAGATAATGCTGCTTCAACGGCAAGAGCTTCCATTGGCAATGAGAAGAGTCCTGATGAATCGACTATCATCTCCAAAATGAAGAGTACTAACATATCCGGACCTGGAACGGCTAAATACCCTCGAGAACCAAGGTATGGACAGCCAGAAAGGCAGCCGCATCAACAGCAAAATAATGCAACTTGGATTCAAGGTGGCAGCAATATGGGTTCTCATGGTGTAAACGATGCAGTTATCGGGGCCGGACAGTTTCATTATGGACAACCTTACAAGTTCTCGGGTGATGGTCAGCCCGTACTTCAGTCTTCCGGTTTTACACCGCCTCTGCTTTACACTGCTACCCAAACGGCTTATATGACTTCACCAGCTCATGTCTATAACATGCAGTCTCCTGCTGTTTATTCTCCTCAGTATGGTTATGGACCATACACCAACATGATCCCTCAGTTTATGCCTGGATACCCTTCCCATGGTTCTGTTCCTGTTGTTGTCAGTCCAGATTTCATTCCTCAGTTATCTGGGCCAAGTGCAGGAAGTGTGGTCCATGGAGGTGAAATGCAGTATGCGGAGAAGCTCTATGTTCCACCAGGACAACCTTCTTTTCCAGATCCTATGTATATGCAGTACTGTCAACAGTCATTTGGGCAGATGGAACCACTGGCTCCTAGGAACCACACCAATGCTCCCGAGTCTCAGAAAGATGATCCAAAGTTCCTTCGACAAATTAGAGGGCCAAGTAACTCTAATATGGCAAGACCGGGAATGGGTGTTAATTACTATGGAATCCAGCCAAATATGGGCATTATGGTGCAGTATCTGCCTACACATCTCGGCCCTCCTCTTTCACCAGGTCATGTTCCTTATGTCGAGGCATATCCTGGATGGCAGCCACAAGGGAGCTTAGAAGGTGCCAATGGCCCTCGATTGTGCAATTTTCTTGAAGAGCTGAAATCTGGGAAAGGTAGGAGGTTTGATCTATCTGACATCACAGGACACATTGTTGAATTCAGTGCGGATCAACATGGGAGCCGATTTATTCAGCAAAAGCTTGAGAATTGTAAACCAGAAGAAAAAGCAGCTGTCTTTAGGGAAATTCTTCCTCATGCTTGCAAACTCATGACAGATGTGTTTGGAAATTATGTCATTCAGAAGTTTTTTGAATATGGAAACTCAACACAGAGAAAGGAACTCGCTGATCAACTCATGGGACAAATAGTACCACTTAGTCTGCAGATGTATGGTTGTCGCGTGATACAAAAGGCTCTTGATGTCATAGAACCTGATCAGAGAGTTCGTTTAGCACGTGAACTAGACGGGCAGGTCATGAGATGTGTTCGGGACCAAAATGGAAACCATGTGATCCAGAAATGCATCGAGAACATCCCGGCAGACAAAGTTGGTTTCATGTTATATGCATTCCGTGGTCAAGTTTCCTCGCTCTCTATGCATCCTTATGGCTGCCGTGTCATACAGAGGCTTTTGGAGCGTTGTTCACATGACCATCAATGTAGGTTCATTACCGAGGAGATACTGGAATCAGTCTGCGTCCTTTCCAAGGACCAATATGGTAATTATGTCACACAGCATGTCTTGGAGAAAGGGACATCTGAAGAAAGGGAGAGGATTGGGAGAAAACTCTCAGGGCACATCGTGCAACTTAGCCTCCACAAATTTGCGTCAAATGTAATAGAGAAGTGTCTGGAGTATGGTGGTCGAGTTGAGCGGGATCTCATCATCAAAGAGATTGCAGGGCCTGACGAGAGCTATAATAGTCTATTGATGATGATGAAGGACCAGTATGGAAACTATGTGGTGCAGAAGATATTCGAGACATGTACGGCTGATCAGCGTTTAACATTGTTCAGCCGAGTGAGAATGCATGCATCTGCATTGAAGAAATACACATATGGGAAACATATTGTTAGTCGTTTGGAACAGCCCTCTATTGAAGAAAACCAAGAATTGAGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632 NABP PUF

Protein Analysis

861

Amino Acids

96.1

Weight (kDa)

6.96

Isoelectric Point (pI)

45.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	536 - 570	1.3e-11	Pumilio-family RNA binding repeat
PUF	PF00806	572 - 605	2e-08	Pumilio-family RNA binding repeat
PUF	PF00806	608 - 641	9.9e-09	Pumilio-family RNA binding repeat
PUF	PF00806	680 - 715	1.2e-10	Pumilio-family RNA binding repeat
PUF	PF00806	718 - 746	2.6e-07	Pumilio-family RNA binding repeat
PUF	PF00806	753 - 786	1.8e-10	Pumilio-family RNA binding repeat
PUF	PF00806	798 - 826	2.4e-09	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25880 AT4G25880 AT4G25880 AT4G25880 AT4G25880
fragaria_vesca	FvH4_4g07600 FvH4_4g07600 FvH4_4g07600
malus_domestica	MD13G1245500.v1.1 MD16G1252800.v1.1
prunus_persica	Prupe.1G079300_v2.0.a1 Prupe.1G079300_v2.0.a1
pyrus_communis	pycom13g21640 pycom16g21410
rosa_chinensis	RchiOBHm_Chr4g0400221
rosa_laevigata	RLG00000009203
rosa_multiflora	Rmu_sc0004338.1_g000002
rosa_roxburghii	Rroxscaffold_5G00345180
rosa_rugosa	Rorug04G0023900 Rorug04G0024000
rosa_samantha	Rh4AG098900 Rh4BG097200 Rh4CG108400 Rh4DG093900
rosa_wichuraiana	Rw4G008220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1931
Acc36I	ACCTGC	1 cut(s)	1921
AccB1I	GGYRCC	1 cut(s)	1520
AccB7I	CCANNNNNTGG	1 cut(s)	749
AccBSI	CCGCTC	1 cut(s)	2335
AccII	CGCG	2 cut(s)	181, 1860
AccIII	TCCGGA	1 cut(s)	710
AciI	CCGC	8 cut(s)	150, 179, 238, 770, 932, 1178, 1625, 2335
AclWI	GGATC	6 cut(s)	1045, 1214, 1310, 1635, 1966, 2346
AcoI	YGGCCR	1 cut(s)	480
AcsI	RAATTY	3 cut(s)	1616, 1704, 2282
AcuI	CTGAAG	2 cut(s)	896, 2241
AcvI	CACGTG	1 cut(s)	1916
AdeI	CACNNNGTG	1 cut(s)	2263
AfaI	GTAC	5 cut(s)	700, 909, 1238, 1830, 2449
AfiI	CCNNNNNNNGG	9 cut(s)	185, 489, 749, 1025, 1082, 1142, 1376, 1409, 2167
AflIII	ACRYGT	1 cut(s)	2444
AgsI	TTSAA	9 cut(s)	325, 546, 630, 803, 1553, 1616, 1772, 2506, 2561
AjnI	CCWGG	7 cut(s)	315, 463, 715, 1066, 1198, 1459, 1489
Alw26I	GTCTC	3 cut(s)	1005, 1308, 2435
AlwI	GGATC	6 cut(s)	1045, 1214, 1310, 1635, 1966, 2346
AlwNI	CAGNNNCTG	2 cut(s)	1429, 2366
Ama87I	CYCGRG	3 cut(s)	735, 889, 1298
Aor13HI	TCCGGA	1 cut(s)	710
AoxI	GGCC	7 cut(s)	480, 852, 1133, 1343, 1444, 1528, 2363
ApeKI	GCWGC	6 cut(s)	623, 766, 810, 1498, 1688, 2070
ApoI	RAATTY	3 cut(s)	1616, 1704, 2282
Asp700I	GAANNNNTTC	2 cut(s)	1707, 2435
AspA2I	CCTAGG	1 cut(s)	1277
AspLEI	GCGC	1 cut(s)	94
AsuC2I	CCSGG	2 cut(s)	1371, 1997
AsuHPI	GGTGA	6 cut(s)	453, 563, 905, 966, 1175, 1449
AvaI	CYCGRG	3 cut(s)	735, 889, 1298
AvaII	GGWCC	6 cut(s)	713, 1034, 1153, 1951, 2170, 2404
AvrII	CCTAGG	1 cut(s)	1277
BaeGI	GKGCMC	1 cut(s)	2259
BanI	GGYRCC	1 cut(s)	1520
BanII	GRGCYC	1 cut(s)	201
BbrPI	CACGTG	1 cut(s)	1916
BbsI	GAAGAC	1 cut(s)	909
BbvI	GCAGC	6 cut(s)	610, 778, 822, 1510, 1700, 2057
BccI	CCATC	6 cut(s)	39, 434, 890, 1255, 1488, 2121
BceAI	ACGGC	5 cut(s)	648, 738, 975, 2058, 2466
BciT130I	CCWGG	7 cut(s)	317, 465, 717, 1068, 1200, 1461, 1491
BciVI	GTATCC	1 cut(s)	1064
BclI	TGATCA	3 cut(s)	1807, 1894, 2455
BcnI	CCSGG	2 cut(s)	1371, 1997
BcoDI	GTCTC	3 cut(s)	1005, 1308, 2435
BfaI	CTAG	4 cut(s)	57, 119, 1278, 1922
BfmI	CTRYAG	1 cut(s)	1841
BfuAI	ACCTGC	1 cut(s)	1921
BfuI	GTATCC	1 cut(s)	1064
BglI	GCCNNNNNGGC	1 cut(s)	763
BisI	GCNGC	8 cut(s)	151, 624, 767, 770, 811, 1499, 1689, 2071
BlnI	CCTAGG	1 cut(s)	1277
BlsI	GCNGC	8 cut(s)	152, 625, 768, 771, 812, 1500, 1690, 2072
BmcAI	AGTACT	2 cut(s)	700, 1238
Bme1390I	CCNGG	9 cut(s)	317, 465, 717, 1068, 1200, 1371, 1461, 1491, 1997
Bme18I	GGWCC	6 cut(s)	713, 1034, 1153, 1951, 2170, 2404
BmeT110I	CYCGRG	3 cut(s)	735, 889, 1298
BmiI	GGNNCC	8 cut(s)	198, 853, 1266, 1275, 1283, 1522, 1640, 1952
BmrFI	CCNGG	9 cut(s)	317, 465, 717, 1068, 1200, 1371, 1461, 1491, 1997
BmsI	GCATC	6 cut(s)	162, 781, 829, 1992, 2068, 2504
BoxI	GACNNNNGTC	1 cut(s)	453
BpiI	GAAGAC	1 cut(s)	909
BpmI	CTGGAG	1 cut(s)	2333
BpuEI	CTTGAG	1 cut(s)	1682
BpuMI	CCSGG	2 cut(s)	1371, 1997
BsaAI	YACGTR	1 cut(s)	1916
BsaJI	CCNNGG	9 cut(s)	316, 355, 743, 1081, 1156, 1277, 2032, 2133, 2166
BsaWI	WCCGGW	2 cut(s)	710, 920
BsaXI	ACNNNNNCTCC	4 cut(s)	1279, 1309, 2259, 2289
Bsc4I	CCNNNNNNNGG	9 cut(s)	185, 489, 749, 1025, 1082, 1142, 1376, 1409, 2167
Bse1I	ACTGG	3 cut(s)	1275, 2148, 2407
Bse3DI	GCAATG	2 cut(s)	43, 658
BseAI	TCCGGA	1 cut(s)	710
BseBI	CCWGG	7 cut(s)	317, 465, 717, 1068, 1200, 1461, 1491
BseDI	CCNNGG	9 cut(s)	316, 355, 743, 1081, 1156, 1277, 2032, 2133, 2166
BseGI	GGATG	6 cut(s)	211, 397, 502, 1499, 1992, 2059
BseLI	CCNNNNNNNGG	9 cut(s)	185, 489, 749, 1025, 1082, 1142, 1376, 1409, 2167
BseMI	GCAATG	2 cut(s)	43, 658
BseMII	CTCAG	9 cut(s)	168, 261, 378, 600, 1034, 1070, 1136, 1319, 2265
BseNI	ACTGG	3 cut(s)	1275, 2148, 2407
BseRI	GAGGAG	4 cut(s)	209, 1008, 1440, 2150
BseSI	GKGCMC	1 cut(s)	2259
BseXI	GCAGC	6 cut(s)	610, 778, 822, 1510, 1700, 2057
BsgI	GTGCAG	3 cut(s)	1161, 1442, 2447
Bsh1236I	CGCG	2 cut(s)	181, 1860
BshFI	GGCC	7 cut(s)	482, 854, 1135, 1345, 1446, 1530, 2365
BshNI	GGYRCC	1 cut(s)	1520
BsiHKCI	CYCGRG	3 cut(s)	735, 889, 1298
BsiSI	CCGG	5 cut(s)	711, 855, 921, 1370, 1997
BslFI	GGGAC	4 cut(s)	337, 1833, 1964, 2227
BslI	CCNNNNNNNGG	9 cut(s)	185, 489, 749, 1025, 1082, 1142, 1376, 1409, 2167
BsmAI	GTCTC	3 cut(s)	1005, 1308, 2435
BsmFI	GGGAC	4 cut(s)	337, 1833, 1964, 2227
BsmI	GAATGC	2 cut(s)	2027, 2493
BsnI	GGCC	7 cut(s)	482, 854, 1135, 1345, 1446, 1530, 2365
BsoBI	CYCGRG	3 cut(s)	735, 889, 1298
Bsp1286I	GDGCHC	2 cut(s)	201, 2259
Bsp13I	TCCGGA	1 cut(s)	710
Bsp19I	CCATGG	2 cut(s)	1081, 1156
BspACI	CCGC	8 cut(s)	150, 179, 238, 770, 932, 1178, 1625, 2335
BspANI	GGCC	7 cut(s)	482, 854, 1135, 1345, 1446, 1530, 2365
BspCNI	CTCAG	9 cut(s)	167, 262, 379, 599, 1033, 1069, 1135, 1318, 2264
BspEI	TCCGGA	1 cut(s)	710
BspFNI	CGCG	2 cut(s)	181, 1860
BspHI	TCATGA	2 cut(s)	1728, 1935
BspLI	GGNNCC	8 cut(s)	198, 853, 1266, 1275, 1283, 1522, 1640, 1952
BspMAI	CTGCAG	1 cut(s)	1845
BspMI	ACCTGC	1 cut(s)	1921
BspPI	GGATC	6 cut(s)	1045, 1214, 1310, 1635, 1966, 2346
BspQI	GCTCTTC	1 cut(s)	1548
BspT107I	GGYRCC	1 cut(s)	1520
BsrBI	CCGCTC	1 cut(s)	2335
BsrDI	GCAATG	2 cut(s)	43, 658
BsrI	ACTGG	3 cut(s)	1275, 2148, 2407
BssECI	CCNNGG	9 cut(s)	316, 355, 743, 1081, 1156, 1277, 2032, 2133, 2166
BssT1I	CCWWGG	6 cut(s)	355, 743, 1081, 1156, 1277, 2166
Bst2UI	CCWGG	7 cut(s)	317, 465, 717, 1068, 1200, 1461, 1491
Bst4CI	ACNGT	4 cut(s)	408, 861, 1241, 1248
Bst6I	CTCTTC	4 cut(s)	547, 653, 689, 1548
BstAPI	GCANNNNNTGC	2 cut(s)	1429, 2263
BstBAI	YACGTR	1 cut(s)	1916
BstC8I	GCNNGC	2 cut(s)	1720, 2493
BstDSI	CCRYGG	3 cut(s)	1081, 1156, 2032
BstENI	CCTNNNNNAGG	1 cut(s)	2165
BstF5I	GGATG	6 cut(s)	211, 397, 502, 1499, 1992, 2059
BstFNI	CGCG	2 cut(s)	181, 1860
BstHHI	GCGC	1 cut(s)	94
BstMAI	GTCTC	3 cut(s)	1005, 1308, 2435
BstMWI	GCNNNNNNNGC	8 cut(s)	237, 632, 763, 778, 1429, 2056, 2095, 2263
BstNI	CCWGG	7 cut(s)	317, 465, 717, 1068, 1200, 1461, 1491
BstNSI	RCATGY	4 cut(s)	997, 2201, 2448, 2495
BstPAI	GACNNNNGTC	1 cut(s)	453
BstSCI	CCNGG	9 cut(s)	315, 463, 715, 1066, 1198, 1369, 1459, 1489, 1995
BstSFI	CTRYAG	1 cut(s)	1841
BstSLI	GKGCMC	1 cut(s)	2259
BstUI	CGCG	2 cut(s)	181, 1860
BstV1I	GCAGC	6 cut(s)	610, 778, 822, 1510, 1700, 2057
BstV2I	GAAGAC	1 cut(s)	909
BstX2I	RGATCY	2 cut(s)	1219, 2338
BstYI	RGATCY	2 cut(s)	1219, 2338
BsuI	GTATCC	1 cut(s)	1064
BsuRI	GGCC	7 cut(s)	482, 854, 1135, 1345, 1446, 1530, 2365
BtgI	CCRYGG	3 cut(s)	1081, 1156, 2032
BtsCI	GGATG	6 cut(s)	211, 397, 502, 1499, 1992, 2059
BtsI	GCAGTG	2 cut(s)	94, 945
BtsIMutI	CAGTG	4 cut(s)	94, 945, 1268, 1627
BveI	ACCTGC	1 cut(s)	1921
Cac8I	GCNNGC	2 cut(s)	1720, 2493
CaiI	CAGNNNCTG	2 cut(s)	1429, 2366
CciI	TCATGA	2 cut(s)	1728, 1935
CfoI	GCGC	1 cut(s)	94
CseI	GACGC	2 cut(s)	2146, 2277
CsiI	ACCWGGT	2 cut(s)	463, 1459
Csp6I	GTAC	5 cut(s)	699, 908, 1237, 1829, 2448
CviQI	GTAC	5 cut(s)	699, 908, 1237, 1829, 2448
DraIII	CACNNNGTG	1 cut(s)	2263
DrdI	GACNNNNNNGTC	1 cut(s)	1931
DseDI	GACNNNNNNGTC	1 cut(s)	1931
EaeI	YGGCCR	1 cut(s)	480
Eam1104I	CTCTTC	4 cut(s)	547, 653, 689, 1548
EarI	CTCTTC	4 cut(s)	547, 653, 689, 1548
Eco130I	CCWWGG	6 cut(s)	355, 743, 1081, 1156, 1277, 2166
Eco24I	GRGCYC	1 cut(s)	201
Eco32I	GATATC	1 cut(s)	27
Eco47I	GGWCC	6 cut(s)	713, 1034, 1153, 1951, 2170, 2404
Eco57I	CTGAAG	2 cut(s)	896, 2241
Eco72I	CACGTG	1 cut(s)	1916
Eco88I	CYCGRG	3 cut(s)	735, 889, 1298
EcoNI	CCTNNNNNAGG	1 cut(s)	2165
EcoO109I	RGGNCCY	1 cut(s)	2363
EcoRI	GAATTC	1 cut(s)	1616
EcoRII	CCWGG	7 cut(s)	315, 463, 715, 1066, 1198, 1459, 1489
EcoRV	GATATC	1 cut(s)	27
EcoT14I	CCWWGG	6 cut(s)	355, 743, 1081, 1156, 1277, 2166
EcoT22I	ATGCAT	5 cut(s)	1985, 2029, 2061, 2493, 2497
EcoT38I	GRGCYC	1 cut(s)	201
ErhI	CCWWGG	6 cut(s)	355, 743, 1081, 1156, 1277, 2166
FalI	AAGNNNNNCTT	2 cut(s)	348, 380
FaqI	GGGAC	4 cut(s)	337, 1833, 1964, 2227
FauI	CCCGC	1 cut(s)	2328
FauNDI	CATATG	1 cut(s)	2518
FbaI	TGATCA	3 cut(s)	1807, 1894, 2455
Fnu4HI	GCNGC	8 cut(s)	151, 624, 767, 770, 811, 1499, 1689, 2071
FokI	GGATG	6 cut(s)	198, 404, 509, 1506, 1979, 2046
FriOI	GRGCYC	1 cut(s)	201
Fsp4HI	GCNGC	8 cut(s)	151, 624, 767, 770, 811, 1499, 1689, 2071
FspBI	CTAG	4 cut(s)	57, 119, 1278, 1922
GlaI	GCGC	1 cut(s)	93
GluI	GCNGC	8 cut(s)	151, 624, 767, 770, 811, 1499, 1689, 2071
GsuI	CTGGAG	1 cut(s)	2333
HaeIII	GGCC	7 cut(s)	482, 854, 1135, 1345, 1446, 1530, 2365
HapII	CCGG	5 cut(s)	711, 855, 921, 1370, 1997
HgaI	GACGC	2 cut(s)	2146, 2277
HhaI	GCGC	1 cut(s)	94
Hin6I	GCGC	1 cut(s)	92
HinP1I	GCGC	1 cut(s)	92
HincII	GTYRAC	1 cut(s)	1244
HindII	GTYRAC	1 cut(s)	1244
HindIII	AAGCTT	1 cut(s)	1658
HinfI	GANTC	7 cut(s)	13, 661, 671, 799, 1301, 1395, 2147
HpaII	CCGG	5 cut(s)	711, 855, 921, 1370, 1997
HphI	GGTGA	6 cut(s)	453, 563, 905, 966, 1175, 1449
Hpy166II	GTNNAC	7 cut(s)	160, 559, 835, 1244, 1673, 1919, 2105
Hpy188I	TCNGA	9 cut(s)	388, 430, 589, 598, 1308, 1594, 1761, 1899, 2221
Hpy8I	GTNNAC	7 cut(s)	160, 559, 835, 1244, 1673, 1919, 2105
HpyAV	CCTTC	8 cut(s)	55, 225, 255, 1086, 1218, 1337, 1511, 2395
HpyCH4III	ACNGT	4 cut(s)	408, 861, 1241, 1248
HpyCH4IV	ACGT	1 cut(s)	1915
HpyF10VI	GCNNNNNNNGC	8 cut(s)	237, 632, 763, 778, 1429, 2056, 2095, 2263
HpySE526I	ACGT	1 cut(s)	1915
HspAI	GCGC	1 cut(s)	92
Kpn2I	TCCGGA	1 cut(s)	710
Ksp22I	TGATCA	3 cut(s)	1807, 1894, 2455
LguI	GCTCTTC	1 cut(s)	1548
LmnI	GCTCC	7 cut(s)	196, 404, 1279, 1300, 1509, 1638, 2095
Lsp1109I	GCAGC	6 cut(s)	610, 778, 822, 1510, 1700, 2057
LweI	GCATC	6 cut(s)	162, 781, 829, 1992, 2068, 2504
MabI	ACCWGGT	2 cut(s)	463, 1459
MaeI	CTAG	4 cut(s)	57, 119, 1278, 1922
MaeII	ACGT	1 cut(s)	1915
MaeIII	GTNAC	7 cut(s)	138, 182, 202, 311, 578, 1349, 2188
MbiI	CCGCTC	1 cut(s)	2335
MflI	RGATCY	2 cut(s)	1219, 2338
MhlI	GDGCHC	2 cut(s)	201, 2259
MlyI	GAGTC	2 cut(s)	670, 1310
Mph1103I	ATGCAT	5 cut(s)	1985, 2029, 2061, 2493, 2497
MroI	TCCGGA	1 cut(s)	710
MroXI	GAANNNNTTC	2 cut(s)	1707, 2435
MseI	TTAA	6 cut(s)	234, 333, 417, 612, 1383, 2465
MslI	CAYNNNNRTG	5 cut(s)	1046, 1161, 1290, 1418, 2064
MspA1I	CMGCKG	1 cut(s)	1691
MspI	CCGG	5 cut(s)	711, 855, 921, 1370, 1997
MspR9I	CCNGG	9 cut(s)	317, 465, 717, 1068, 1200, 1371, 1461, 1491, 1997
Mva1269I	GAATGC	2 cut(s)	2027, 2493
MvaI	CCWGG	7 cut(s)	317, 465, 717, 1068, 1200, 1461, 1491
MvnI	CGCG	2 cut(s)	181, 1860
MwoI	GCNNNNNNNGC	8 cut(s)	237, 632, 763, 778, 1429, 2056, 2095, 2263
NciI	CCSGG	2 cut(s)	1371, 1997
NcoI	CCATGG	2 cut(s)	1081, 1156
NdeI	CATATG	1 cut(s)	2518
NlaIV	GGNNCC	8 cut(s)	198, 853, 1266, 1275, 1283, 1522, 1640, 1952
NmeAIII	GCCGAG	2 cut(s)	1422, 2504
NmuCI	GTSAC	3 cut(s)	138, 202, 2188
NsiI	ATGCAT	5 cut(s)	1985, 2029, 2061, 2493, 2497
NspI	RCATGY	4 cut(s)	997, 2201, 2448, 2495
PaeI	GCATGC	1 cut(s)	2495
PaeR7I	CTCGAG	1 cut(s)	735
PagI	TCATGA	2 cut(s)	1728, 1935
PciI	ACATGT	1 cut(s)	2444
PciSI	GCTCTTC	1 cut(s)	1548
PcsI	WCGNNNNNNNCGW	1 cut(s)	1912
PctI	GAATGC	2 cut(s)	2027, 2493
PdmI	GAANNNNTTC	2 cut(s)	1707, 2435
PfeI	GAWTC	5 cut(s)	13, 671, 799, 1395, 2147
PflMI	CCANNNNNTGG	1 cut(s)	749
PfoI	TCCNGGA	1 cut(s)	1489
PkrI	GCNGC	8 cut(s)	152, 625, 768, 771, 812, 1500, 1690, 2072
PleI	GAGTC	2 cut(s)	669, 1309
PmaCI	CACGTG	1 cut(s)	1916
PmlI	CACGTG	1 cut(s)	1916
PpsI	GAGTC	2 cut(s)	669, 1309
Ppu21I	YACGTR	1 cut(s)	1916
PscI	ACATGT	1 cut(s)	2444
PshAI	GACNNNNGTC	1 cut(s)	453
Psp6I	CCWGG	7 cut(s)	315, 463, 715, 1066, 1198, 1459, 1489
PspCI	CACGTG	1 cut(s)	1916
PspGI	CCWGG	7 cut(s)	315, 463, 715, 1066, 1198, 1459, 1489
PspN4I	GGNNCC	8 cut(s)	198, 853, 1266, 1275, 1283, 1522, 1640, 1952
PstI	CTGCAG	1 cut(s)	1845
PstNI	CAGNNNCTG	2 cut(s)	1429, 2366
PsuI	RGATCY	2 cut(s)	1219, 2338
PvuII	CAGCTG	1 cut(s)	1691
RsaI	GTAC	5 cut(s)	700, 909, 1238, 1830, 2449
RsaNI	GTAC	5 cut(s)	699, 908, 1237, 1829, 2448
RseI	CAYNNNNRTG	5 cut(s)	1046, 1161, 1290, 1418, 2064
SapI	GCTCTTC	1 cut(s)	1548
SaqAI	TTAA	6 cut(s)	234, 333, 417, 612, 1383, 2465
SatI	GCNGC	8 cut(s)	151, 624, 767, 770, 811, 1499, 1689, 2071
ScaI	AGTACT	2 cut(s)	700, 1238
SchI	GAGTC	2 cut(s)	670, 1310
ScrFI	CCNGG	9 cut(s)	317, 465, 717, 1068, 1200, 1371, 1461, 1491, 1997
SduI	GDGCHC	2 cut(s)	201, 2259
SexAI	ACCWGGT	2 cut(s)	463, 1459
SfaNI	GCATC	6 cut(s)	162, 781, 829, 1992, 2068, 2504
SfcI	CTRYAG	1 cut(s)	1841
Sfr274I	CTCGAG	1 cut(s)	735
SinI	GGWCC	6 cut(s)	713, 1034, 1153, 1951, 2170, 2404
SlaI	CTCGAG	1 cut(s)	735
SmiMI	CAYNNNNRTG	5 cut(s)	1046, 1161, 1290, 1418, 2064
SmlI	CTYRAG	2 cut(s)	735, 1661
SmoI	CTYRAG	2 cut(s)	735, 1661
SphI	GCATGC	1 cut(s)	2495
SsiI	CCGC	8 cut(s)	150, 179, 238, 770, 932, 1178, 1625, 2335
SspMI	CTAG	4 cut(s)	57, 119, 1278, 1922
StyD4I	CCNGG	9 cut(s)	315, 463, 715, 1066, 1198, 1369, 1459, 1489, 1995
StyI	CCWWGG	6 cut(s)	355, 743, 1081, 1156, 1277, 2166
TaaI	ACNGT	4 cut(s)	408, 861, 1241, 1248
TaiI	ACGT	1 cut(s)	1918
TaqI	TCGA	9 cut(s)	79, 674, 736, 1330, 1479, 1534, 1986, 2326, 2439
TaqII	GACCGA	1 cut(s)	522
TatI	WGTACW	2 cut(s)	698, 1236
TauI	GCSGC	2 cut(s)	153, 772
TfiI	GAWTC	5 cut(s)	13, 671, 799, 1395, 2147
Tru1I	TTAA	6 cut(s)	234, 333, 417, 612, 1383, 2465
Tru9I	TTAA	6 cut(s)	234, 333, 417, 612, 1383, 2465
TscAI	CASTG	4 cut(s)	94, 952, 1275, 1627
TseFI	GTSAC	3 cut(s)	138, 202, 2188
TseI	GCWGC	6 cut(s)	623, 766, 810, 1498, 1688, 2070
Tsp45I	GTSAC	3 cut(s)	138, 202, 2188
TspGWI	ACGGA	1 cut(s)	2021
TspRI	CASTG	4 cut(s)	94, 952, 1275, 1627
Van91I	CCANNNNNTGG	1 cut(s)	749
VpaK11BI	GGWCC	6 cut(s)	713, 1034, 1153, 1951, 2170, 2404
XagI	CCTNNNNNAGG	1 cut(s)	2165
XapI	RAATTY	3 cut(s)	1616, 1704, 2282
XceI	RCATGY	4 cut(s)	997, 2201, 2448, 2495
XhoI	CTCGAG	1 cut(s)	735
XmaJI	CCTAGG	1 cut(s)	1277
XmnI	GAANNNNTTC	2 cut(s)	1707, 2435
XspI	CTAG	4 cut(s)	57, 119, 1278, 1922
ZrmI	AGTACT	2 cut(s)	700, 1238
Zsp2I	ATGCAT	5 cut(s)	1985, 2029, 2061, 2493, 2497

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.