Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0007034

Physical Location & Seq

Genomic Coordinates Forward (+)

15513038 .. 15522633

Gene Structure 9596 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00345180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2943 bp

ATGGCGACTGAGAGTCCAATTAGAATGTCAGAAACTGGTGGAAACTGGGGTTCCCATAAGAAGCCGGCAAAACTTGCATCATCATCTGCTAATATGACAGCAGAGGAGTTGAAATTGCTTCTGAGAGACCATAGGTTTCATAGCAGCAGAAAGGATGTTGCCCCCAATCGAAGTGGAAGTGCACCACCAAGTATGGAGGGCTCATTTTTGGCCATTAATAATCTGATATCCCAGCAGGACTCTGGCATGACTGGAAGCTTGGCTAGTTTAACTAGTGTTATTGAAAGGTGCGAGTCTGAGGAGCAACTGCGTTCAGATCCAGCTTATCTGGCTTATTACTGTGCCAATGTCAATCTGAATCCAAGACTTCCTTCTCCCCTTATCTCATGGGAGAATCGACGTCTGGCACGCCATATCGGTAGTTTCAGTCCAAACTTGGGACCCGTTGATGACAGTGGTAATGGTTCATTGCATATGTCTCCAGGACTACTGCCTACACACGAGGAAGAGTCAGAGGATGATCAGACACCTCAACAGACTTCTAATGATTGGGTAGATCAGACAAGTTCTGGAGAGGAAGCAGCTCAATTGATAGGACGACAGAAAAATGAAGATTCTTGTGGGTCTACTTCACCTGTAGATAATCATTCACATACCTCAAGCAATGGGGTACCTGAGGAGTTTAATCAGAGTGCTGTTTCCAATTCCTTGCATGATCTCCCTAGGCCCAATGTAGATGAAGAACGTGCAGCTGATACAGTGCTTTCACTAAATGATGAAGCCTCATCCAGTCCTGTTGCAATTTCGTCGTCTCATGTTTATACCAGGAGCACAGGTGTTAATGATGCAGGTGTTTCCCTTGTTGAATCTGGAATGAAGTCTCTTAATGTATCAAACATGCCAGAAAATCAAAAGAATCAAGTACAGTGGCCGCCCAGCTACCAGAATAATTTCCTGCAATACCAGATACAGCAACAGCAAATCAATTTATGCCAAGTTCAAAATGCCACATCTCAGATAACTCCTCAAGGAGCTCACTGCACATATATTGGCATGGATCACTATCACCCTAATACCTCCAAATTTGCAGCGGATGCACAGCCGGTACTCCAGACATCAGGGTTCGCACATCCACTTTATGCAAACGCAGCAGCCTACACGAGTGCAACACATCCGTGGATATGCTTTAAACCCCCTGGTTTTTCTCCATATGTTGGTGGATATCATCCTCCAGGGGCTGTTCCTGTTGTTGTTGAGGGAACTGTGGGTTCAAGCTTTAATACTCGAACATCTGCGGGAAGCATTTCACCTGGGGCTGATGTGCAACATATAAGCAAGTCATATGGGCAGCCTGGGTTTCCTCTGCAAACTTCTTATTCCGATCCTATGTATATGCAATACTATCAACAGCCGTTTGTGGAGTCATATGGTGTTTCTGGCCAGTATGCTCCATTGGCATCTAGAGGTGGTCTTGACTTGAACAAAGCGTCCAATCATGCTGCTAGTTTGGATGACCACAAAATCCAACATCCGAGGAATGGAGGGCCGGGGAATTTGAACTCACAAAGAGGACCAGTAAGTACCAGCTATTATGGAAGTGCACCAAATGTTGGTGTTTTGATGCAGTATCCTACCTCACCCCTAAATAGTCCTGTTCTGCCAACATCATCCATAAGTTCAGGAAGAAATACTGGCTTATATCATGGATGGCCAGGGCACCGAGGCTTTGAGAGTCTTCATGACCCCAAAATTTATAATTTTCTCGAGGAGTTGAAATCTGGCAAAGGCCGCAGATTTGAGCTATCTGATATCACAGGACATATTGTTGAATTTAGTGCTGATCAACATGGGAGTCGATTTATCCAGCAGAAGTTGGAAAACTGTAGTATTGAAGAGAAGGCTTCTGTATTCAGAGAAGTTCTCCCACATGCTTGTCAATTAATGACTGATGTTTTTGGTAACTATGTGATTCAAAAGTTTTTTGAGTATGGAAGCCCTCAGCAAAGGAAGGATCTTGCCAATCAACTTTCAGGCCAAATTTTGCCTCTAAGTCTGCAGATGTATGGCTGTCGTGTAATCCAAAAGGGATTTGAAGGCGCTGAAGAGGCCTTTGATATCAAGTGTCCCAGTGACGTTTGTGTCAAACTGAATGCTGATTCCTGTGGTTGTAAACTTGCACTTGATGTGATTGAGCTTGAACAGAAAGCAAGGCTTGTACATGAGTTGGATGGACATGTTATGAGATGTGTTCGTGACCAAAATGGAAACCATGTCATACAAAAGTGTATTGAGAGCATCCCAACAGAAAAGATTGGGTTTATAATATCTGCTTTCCATGATCAAGTTGCAACACTTTCAATGCATCCCTATGGTTGCCGAGTTATACAGAGAGTTCTGGAGCATTGTACAAATGAGTTGCAATGCCAGTTTATAGTTGATGAGATCTTGGACTCTGTGCATACTCTTGCTCAGGACCAGTATGGCAATTATGTCTCCCAGCATGTATTGGAGAGGGGGAAACCTCATGAAAGAAGCCGAATTATTAGCAAGTTGTCGGGTCATATTGTACAGCTGAGCCAGCACAAATTTGCATCCAATGTTGTTGAGAAATGTTTGGAGTATGGTGATGCTGCAGAACGAGAACTGTTGATAAGGGAGATTGTTGGCCCTAATGATGGAAATGATAACTTATTGATAATGATGAAGGACCAATTTGCTAATTATGTGGTGCAGAAGGCTCTTGAAATCTGTACAGACAGTCAACGAACGATATTGATTAATCGCATAAGGGCTCATACTCATGCGTTGAAGAAATACACTTATGGGAAACACATTGTTGCTCGATTTGAGCAGCTATTTGGAGAAGGTGCTTACTATGCGCAGAATCTGAAGGAAAAGCAAGCAGTAGGTGCAGATGGGGGCGTTATTGAACCTGAGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632 NABP PUF

Protein Analysis

980

Amino Acids

107.78

Weight (kDa)

5.85

Isoelectric Point (pI)

46.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	607 - 641	1.9e-10	Pumilio-family RNA binding repeat
PUF	PF00806	643 - 676	3.5e-09	Pumilio-family RNA binding repeat
PUF_NOP9	PF22493	742 - 826	9.8e-06	NOP9-like PUF repeat domain
PUF	PF00806	781 - 816	1.7e-13	Pumilio-family RNA binding repeat
PUF	PF00806	819 - 851	5.7e-07	Pumilio-family RNA binding repeat
PUF	PF00806	854 - 887	7.1e-11	Pumilio-family RNA binding repeat
PUF	PF00806	900 - 929	4.9e-08	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25880 AT4G25880 AT4G25880 AT4G25880 AT4G25880
fragaria_vesca	FvH4_4g07600 FvH4_4g07600 FvH4_4g07600
malus_domestica	MD13G1245500.v1.1 MD16G1252800.v1.1
prunus_persica	Prupe.1G079300_v2.0.a1 Prupe.1G079300_v2.0.a1
pyrus_communis	pycom13g21640 pycom16g21410
rosa_chinensis	RchiOBHm_Chr4g0400221
rosa_laevigata	RLG00000009203
rosa_multiflora	Rmu_sc0004338.1_g000002
rosa_roxburghii	Rroxscaffold_5G00345180
rosa_rugosa	Rorug04G0023900 Rorug04G0024000
rosa_samantha	Rh4AG098900 Rh4BG097200 Rh4CG108400 Rh4DG093900
rosa_wichuraiana	Rw4G008220

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1755, 2321
AarI	CACCTGC	1 cut(s)	839
AasI	GACNNNNNNGTC	1 cut(s)	2138
AatII	GACGTC	1 cut(s)	403
Acc16I	TGCGCA	1 cut(s)	2880
Acc36I	ACCTGC	1 cut(s)	839
Acc65I	GGTACC	1 cut(s)	670
AccB1I	GGYRCC	2 cut(s)	670, 1716
AccB7I	CCANNNNNTGG	2 cut(s)	1214, 1610
AccI	GTMKAC	1 cut(s)	626
AciI	CCGC	4 cut(s)	932, 1091, 1295, 1789
AclWI	GGATC	4 cut(s)	311, 1065, 1376, 2019
AcoI	YGGCCR	4 cut(s)	210, 929, 1438, 1709
AcsI	RAATTY	6 cut(s)	1082, 1552, 1749, 1829, 2037, 2585
AcuI	CTGAAG	2 cut(s)	2121, 2909
AcyI	GRCGYC	1 cut(s)	400
AfaI	GTAC	8 cut(s)	672, 924, 1107, 1582, 2217, 2407, 2568, 2752
AfiI	CCNNNNNNNGG	5 cut(s)	437, 1214, 1538, 1610, 2675
AflIII	ACRYGT	1 cut(s)	2233
AhdI	GACNNNNNGTC	1 cut(s)	12
AhlI	ACTAGT	1 cut(s)	272
AjnI	CCWGG	7 cut(s)	481, 824, 1195, 1231, 1309, 1351, 1711
AjuI	GAANNNNNNNTTGG	2 cut(s)	355, 387
AleI	CACNNNNGTG	1 cut(s)	1174
AloI	GAACNNNNNNTCC	2 cut(s)	1472, 1504
Alw21I	GWGCWC	4 cut(s)	184, 833, 1036, 1603
Alw26I	GTCTC	5 cut(s)	120, 483, 816, 885, 2497
Alw44I	GTGCAC	2 cut(s)	180, 1599
AlwI	GGATC	4 cut(s)	311, 1065, 1376, 2019
AlwNI	CAGNNNCTG	3 cut(s)	35, 1238, 2887
Ama87I	CYCGRG	1 cut(s)	1763
ApaLI	GTGCAC	2 cut(s)	180, 1599
ApoI	RAATTY	6 cut(s)	1082, 1552, 1749, 1829, 2037, 2585
AseI	ATTAAT	3 cut(s)	216, 1940, 2778
Asp700I	GAANNNNTTC	2 cut(s)	950, 1303
Asp718I	GGTACC	1 cut(s)	670
AspA2I	CCTAGG	1 cut(s)	722
AspLEI	GCGC	2 cut(s)	2099, 2881
AspS9I	GGNCC	7 cut(s)	440, 726, 1544, 1571, 2473, 2666, 2707
AsuC2I	CCSGG	1 cut(s)	1548
AsuHPI	GGTGA	5 cut(s)	624, 1058, 1299, 1629, 2636
AvaI	CYCGRG	1 cut(s)	1763
AvaII	GGWCC	4 cut(s)	440, 1571, 2473, 2707
AvrII	CCTAGG	1 cut(s)	722
AxyI	CCTNAGG	1 cut(s)	675
BaeGI	GKGCMC	3 cut(s)	184, 1603, 1719
BalI	TGGCCA	3 cut(s)	212, 1440, 1711
BanI	GGYRCC	2 cut(s)	670, 1716
BanII	GRGCYC	3 cut(s)	203, 1036, 2794
BauI	CACGAG	2 cut(s)	500, 1159
BbsI	GAAGAC	1 cut(s)	1727
Bbv12I	GWGCWC	4 cut(s)	184, 833, 1036, 1603
BbvCI	CCTCAGC	1 cut(s)	1998
BccI	CCATC	4 cut(s)	1701, 2222, 2669, 2909
BceAI	ACGGC	1 cut(s)	1396
BcgI	CGANNNNNNTGC	2 cut(s)	789, 823
BciT130I	CCWGG	7 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1713
BciVI	GTATCC	1 cut(s)	1638
BclI	TGATCA	3 cut(s)	520, 1840, 2338
BcnI	CCSGG	1 cut(s)	1548
BcoDI	GTCTC	5 cut(s)	120, 483, 816, 885, 2497
BcuI	ACTAGT	1 cut(s)	272
BfaI	CTAG	5 cut(s)	264, 273, 723, 1461, 1503
BfmI	CTRYAG	4 cut(s)	636, 1882, 2054, 2631
BfoI	RGCGCY	1 cut(s)	2100
BfuAI	ACCTGC	1 cut(s)	839
BfuI	GTATCC	1 cut(s)	1638
BglII	AGATCT	1 cut(s)	2442
BlnI	CCTAGG	1 cut(s)	722
BlpI	GCTNAGC	1 cut(s)	2573
Bme1390I	CCNGG	8 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1548, 1713
Bme18I	GGWCC	4 cut(s)	440, 1571, 2473, 2707
BmeRI	GACNNNNNGTC	1 cut(s)	12
BmeT110I	CYCGRG	1 cut(s)	1763
BmgT120I	GGNCC	7 cut(s)	440, 726, 1544, 1571, 2473, 2666, 2707
BmiI	GGNNCC	5 cut(s)	52, 441, 442, 672, 1718
BmrFI	CCNGG	8 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1548, 1713
BmrI	ACTGGG	2 cut(s)	55, 2121
BmsI	GCATC	9 cut(s)	86, 835, 1084, 1466, 1611, 2304, 2371, 2600, 2617
BmuI	ACTGGG	2 cut(s)	55, 2121
BpiI	GAAGAC	1 cut(s)	1727
BplI	GAGNNNNNCTC	2 cut(s)	1905, 1937
BpmI	CTGGAG	5 cut(s)	465, 591, 1094, 1215, 2417
Bpu10I	CCTNAGC	2 cut(s)	1998, 2469
Bpu1102I	GCTNAGC	1 cut(s)	2573
BpuEI	CTTGAG	2 cut(s)	643, 1011
BpuMI	CCSGG	1 cut(s)	1548
BsaBI	GATNNNNATC	1 cut(s)	1062
BsaHI	GRCGYC	1 cut(s)	400
BsaI	GGTCTC	1 cut(s)	120
BsaXI	ACNNNNNCTCC	2 cut(s)	820, 850
Bsc4I	CCNNNNNNNGG	5 cut(s)	437, 1214, 1538, 1610, 2675
Bse118I	RCCGGY	2 cut(s)	64, 1102
Bse21I	CCTNAGG	1 cut(s)	675
Bse3DI	GCAATG	3 cut(s)	467, 670, 2426
Bse8I	GATNNNNATC	1 cut(s)	1062
BseBI	CCWGG	7 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1713
BseJI	GATNNNNATC	1 cut(s)	1062
BseLI	CCNNNNNNNGG	5 cut(s)	437, 1214, 1538, 1610, 2675
BseMI	GCAATG	3 cut(s)	467, 670, 2426
BseMII	CTCAG	8 cut(s)	113, 288, 666, 1028, 2012, 2483, 2564, 2925
BseRI	GAGGAG	5 cut(s)	119, 314, 692, 1014, 1781
BseSI	GKGCMC	3 cut(s)	184, 1603, 1719
BseYI	CCCAGC	3 cut(s)	231, 935, 2496
BsgI	GTGCAG	4 cut(s)	768, 1024, 2750, 2931
BshNI	GGYRCC	2 cut(s)	670, 1716
BsiHKAI	GWGCWC	4 cut(s)	184, 833, 1036, 1603
BsiHKCI	CYCGRG	1 cut(s)	1763
BsiSI	CCGG	3 cut(s)	65, 1103, 1547
BslFI	GGGAC	2 cut(s)	453, 2109
BslI	CCNNNNNNNGG	5 cut(s)	437, 1214, 1538, 1610, 2675
BsmAI	GTCTC	5 cut(s)	120, 483, 816, 885, 2497
BsmBI	CGTCTC	1 cut(s)	816
BsmFI	GGGAC	2 cut(s)	453, 2109
BsmI	GAATGC	1 cut(s)	2155
Bso31I	GGTCTC	1 cut(s)	120
BsoBI	CYCGRG	1 cut(s)	1763
Bsp1286I	GDGCHC	7 cut(s)	184, 203, 833, 1036, 1603, 1719, 2794
Bsp1407I	TGTACA	4 cut(s)	2215, 2405, 2566, 2750
Bsp1720I	GCTNAGC	1 cut(s)	2573
BspACI	CCGC	4 cut(s)	932, 1091, 1295, 1789
BspCNI	CTCAG	8 cut(s)	114, 289, 667, 1027, 2011, 2482, 2565, 2926
BspHI	TCATGA	2 cut(s)	1738, 2524
BspLI	GGNNCC	5 cut(s)	52, 441, 442, 672, 1718
BspMAI	CTGCAG	2 cut(s)	2058, 2635
BspMI	ACCTGC	1 cut(s)	839
BspPI	GGATC	4 cut(s)	311, 1065, 1376, 2019
BspT107I	GGYRCC	2 cut(s)	670, 1716
BspTNI	GGTCTC	1 cut(s)	120
BsrDI	GCAATG	3 cut(s)	467, 670, 2426
BsrFI	RCCGGY	2 cut(s)	64, 1102
BsrGI	TGTACA	4 cut(s)	2215, 2405, 2566, 2750
BssAI	RCCGGY	2 cut(s)	64, 1102
BssNI	GRCGYC	1 cut(s)	400
BssSI	CACGAG	2 cut(s)	500, 1159
BssT1I	CCWWGG	1 cut(s)	722
Bst2BI	CACGAG	2 cut(s)	500, 1159
Bst2UI	CCWGG	7 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1713
Bst4CI	ACNGT	8 cut(s)	341, 455, 760, 927, 1264, 1883, 2646, 2759
Bst6I	CTCTTC	3 cut(s)	501, 1887, 2097
BstACI	GRCGYC	1 cut(s)	400
BstAPI	GCANNNNNTGC	3 cut(s)	74, 1094, 2909
BstAUI	TGTACA	4 cut(s)	2215, 2405, 2566, 2750
BstC8I	GCNNGC	4 cut(s)	66, 409, 2579, 2901
BstDSI	CCRYGG	1 cut(s)	1175
BstH2I	RGCGCY	1 cut(s)	2100
BstHHI	GCGC	2 cut(s)	2099, 2881
BstMAI	GTCTC	5 cut(s)	120, 483, 816, 885, 2497
BstNI	CCWGG	7 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1713
BstNSI	RCATGY	4 cut(s)	901, 1931, 2237, 2504
BstSCI	CCNGG	8 cut(s)	481, 824, 1195, 1231, 1309, 1351, 1546, 1711
BstSFI	CTRYAG	4 cut(s)	636, 1882, 2054, 2631
BstSLI	GKGCMC	3 cut(s)	184, 1603, 1719
BstV2I	GAAGAC	1 cut(s)	1727
BstX2I	RGATCY	3 cut(s)	316, 2011, 2442
BstYI	RGATCY	3 cut(s)	316, 2011, 2442
Bsu36I	CCTNAGG	1 cut(s)	675
BsuI	GTATCC	1 cut(s)	1638
BtgI	CCRYGG	1 cut(s)	1175
BtsI	GCAGTG	1 cut(s)	1036
BtsIMutI	CAGTG	5 cut(s)	460, 765, 932, 1036, 2134
BveI	ACCTGC	1 cut(s)	839
Cac8I	GCNNGC	4 cut(s)	66, 409, 2579, 2901
CaiI	CAGNNNCTG	3 cut(s)	35, 1238, 2887
CciI	TCATGA	2 cut(s)	1738, 2524
CfoI	GCGC	2 cut(s)	2099, 2881
Cfr10I	RCCGGY	2 cut(s)	64, 1102
Cfr13I	GGNCC	7 cut(s)	440, 726, 1544, 1571, 2473, 2666, 2707
CseI	GACGC	1 cut(s)	1476
Csp6I	GTAC	8 cut(s)	671, 923, 1106, 1581, 2216, 2406, 2567, 2751
CspCI	CAANNNNNGTGG	4 cut(s)	154, 189, 908, 943
CviQI	GTAC	8 cut(s)	671, 923, 1106, 1581, 2216, 2406, 2567, 2751
DraI	TTTAAA	1 cut(s)	1189
DrdI	GACNNNNNNGTC	1 cut(s)	2138
DriI	GACNNNNNGTC	1 cut(s)	12
DseDI	GACNNNNNNGTC	1 cut(s)	2138
EaeI	YGGCCR	4 cut(s)	210, 929, 1438, 1709
Eam1104I	CTCTTC	3 cut(s)	501, 1887, 2097
Eam1105I	GACNNNNNGTC	1 cut(s)	12
EarI	CTCTTC	3 cut(s)	501, 1887, 2097
Ecl136II	GAGCTC	1 cut(s)	1034
Eco130I	CCWWGG	1 cut(s)	722
Eco147I	AGGCCT	1 cut(s)	2108
Eco24I	GRGCYC	3 cut(s)	203, 1036, 2794
Eco31I	GGTCTC	1 cut(s)	120
Eco32I	GATATC	4 cut(s)	228, 1223, 1810, 2116
Eco47I	GGWCC	4 cut(s)	440, 1571, 2473, 2707
Eco53kI	GAGCTC	1 cut(s)	1034
Eco57I	CTGAAG	2 cut(s)	2121, 2909
Eco81I	CCTNAGG	1 cut(s)	675
Eco88I	CYCGRG	1 cut(s)	1763
EcoICRI	GAGCTC	1 cut(s)	1034
EcoO109I	RGGNCCY	1 cut(s)	440
EcoRII	CCWGG	7 cut(s)	481, 824, 1195, 1231, 1309, 1351, 1711
EcoRV	GATATC	4 cut(s)	228, 1223, 1810, 2116
EcoT14I	CCWWGG	1 cut(s)	722
EcoT22I	ATGCAT	1 cut(s)	2364
EcoT38I	GRGCYC	3 cut(s)	203, 1036, 2794
ErhI	CCWWGG	1 cut(s)	722
Esp3I	CGTCTC	1 cut(s)	816
FalI	AAGNNNNNCTT	4 cut(s)	355, 387, 2196, 2228
FaqI	GGGAC	2 cut(s)	453, 2109
FauI	CCCGC	1 cut(s)	1288
FauNDI	CATATG	4 cut(s)	474, 1210, 1342, 1426
FbaI	TGATCA	3 cut(s)	520, 1840, 2338
FblI	GTMKAC	1 cut(s)	626
FriOI	GRGCYC	3 cut(s)	203, 1036, 2794
FspBI	CTAG	5 cut(s)	264, 273, 723, 1461, 1503
FspI	TGCGCA	1 cut(s)	2880
GlaI	GCGC	2 cut(s)	2098, 2880
GsaI	CCCAGC	3 cut(s)	235, 939, 2500
GsuI	CTGGAG	5 cut(s)	465, 591, 1094, 1215, 2417
HaeII	RGCGCY	1 cut(s)	2100
HapII	CCGG	3 cut(s)	65, 1103, 1547
HgaI	GACGC	1 cut(s)	1476
HhaI	GCGC	2 cut(s)	2099, 2881
Hin1I	GRCGYC	1 cut(s)	400
Hin6I	GCGC	2 cut(s)	2097, 2879
HinP1I	GCGC	2 cut(s)	2097, 2879
HincII	GTYRAC	1 cut(s)	2762
HindII	GTYRAC	1 cut(s)	2762
HindIII	AAGCTT	2 cut(s)	256, 1273
HpaII	CCGG	3 cut(s)	65, 1103, 1547
HphI	GGTGA	5 cut(s)	624, 1058, 1299, 1629, 2636
Hpy166II	GTNNAC	5 cut(s)	182, 627, 1601, 2171, 2762
Hpy8I	GTNNAC	5 cut(s)	182, 627, 1601, 2171, 2762
Hpy99I	CGWCG	2 cut(s)	402, 811
HpyAV	CCTTC	8 cut(s)	381, 1891, 2002, 2087, 2698, 2728, 2858, 2884
HpyCH4III	ACNGT	8 cut(s)	341, 455, 760, 927, 1264, 1883, 2646, 2759
HpyCH4IV	ACGT	3 cut(s)	400, 745, 2133
HpySE526I	ACGT	3 cut(s)	400, 745, 2133
Hsp92I	GRCGYC	1 cut(s)	400
HspAI	GCGC	2 cut(s)	2097, 2879
KflI	GGGWCCC	1 cut(s)	440
KpnI	GGTACC	1 cut(s)	674
KroI	GCCGGC	1 cut(s)	64
KroNI	GCCGGC	1 cut(s)	66
Ksp22I	TGATCA	3 cut(s)	520, 1840, 2338
LmnI	GCTCC	5 cut(s)	301, 828, 1031, 1453, 2398
LweI	GCATC	9 cut(s)	86, 835, 1084, 1466, 1611, 2304, 2371, 2600, 2617
MaeI	CTAG	5 cut(s)	264, 273, 723, 1461, 1503
MaeII	ACGT	3 cut(s)	400, 745, 2133
MaeIII	GTNAC	3 cut(s)	1958, 2129, 2252
MboII	GAAGA	8 cut(s)	518, 623, 752, 1695, 1727, 1904, 2114, 2821
MfeI	CAATTG	1 cut(s)	587
MflI	RGATCY	3 cut(s)	316, 2011, 2442
MhlI	GDGCHC	7 cut(s)	184, 203, 833, 1036, 1603, 1719, 2794
MlsI	TGGCCA	3 cut(s)	212, 1440, 1711
MluNI	TGGCCA	3 cut(s)	212, 1440, 1711
MlyI	GAGTC	8 cut(s)	22, 233, 302, 518, 1430, 1741, 1861, 2444
MmeI	TCCRAC	3 cut(s)	1549, 1854, 2205
Mox20I	TGGCCA	3 cut(s)	212, 1440, 1711
Mph1103I	ATGCAT	1 cut(s)	2364
MroNI	GCCGGC	1 cut(s)	64
MroXI	GAANNNNTTC	2 cut(s)	950, 1303
MscI	TGGCCA	3 cut(s)	212, 1440, 1711
MseI	TTAA	9 cut(s)	216, 269, 684, 840, 885, 1188, 1278, 1940, 2778
MslI	CAYNNNNRTG	3 cut(s)	1174, 2367, 2799
Msp20I	TGGCCA	3 cut(s)	212, 1440, 1711
MspA1I	CMGCKG	3 cut(s)	752, 1091, 2572
MspI	CCGG	3 cut(s)	65, 1103, 1547
MspR9I	CCNGG	8 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1548, 1713
MunI	CAATTG	1 cut(s)	587
Mva1269I	GAATGC	1 cut(s)	2155
MvaI	CCWGG	7 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1713
NaeI	GCCGGC	1 cut(s)	66
NciI	CCSGG	1 cut(s)	1548
NdeI	CATATG	4 cut(s)	474, 1210, 1342, 1426
NgoMIV	GCCGGC	1 cut(s)	64
NlaIV	GGNNCC	5 cut(s)	52, 441, 442, 672, 1718
NmeAIII	GCCGAG	1 cut(s)	2402
NmuCI	GTSAC	2 cut(s)	2129, 2252
NsbI	TGCGCA	1 cut(s)	2880
NsiI	ATGCAT	1 cut(s)	2364
NspI	RCATGY	4 cut(s)	901, 1931, 2237, 2504
OliI	CACNNNNGTG	1 cut(s)	1174
PaeR7I	CTCGAG	1 cut(s)	1763
PagI	TCATGA	2 cut(s)	1738, 2524
PaqCI	CACCTGC	1 cut(s)	839
PceI	AGGCCT	1 cut(s)	2108
PciI	ACATGT	1 cut(s)	2233
PctI	GAATGC	1 cut(s)	2155
PdiI	GCCGGC	1 cut(s)	66
PdmI	GAANNNNTTC	2 cut(s)	950, 1303
PfeI	GAWTC	8 cut(s)	358, 394, 614, 866, 916, 1969, 2156, 2884
PflMI	CCANNNNNTGG	2 cut(s)	1214, 1610
PfoI	TCCNGGA	1 cut(s)	481
PleI	GAGTC	8 cut(s)	21, 233, 301, 517, 1429, 1740, 1860, 2444
PpsI	GAGTC	8 cut(s)	21, 233, 301, 517, 1429, 1740, 1860, 2444
PpuMI	RGGWCCY	1 cut(s)	440
PscI	ACATGT	1 cut(s)	2233
PshBI	ATTAAT	3 cut(s)	216, 1940, 2778
PsiI	TTATAA	2 cut(s)	1755, 2321
Psp124BI	GAGCTC	1 cut(s)	1036
Psp5II	RGGWCCY	1 cut(s)	440
Psp6I	CCWGG	7 cut(s)	481, 824, 1195, 1231, 1309, 1351, 1711
PspFI	CCCAGC	3 cut(s)	231, 935, 2496
PspGI	CCWGG	7 cut(s)	481, 824, 1195, 1231, 1309, 1351, 1711
PspN4I	GGNNCC	5 cut(s)	52, 441, 442, 672, 1718
PspPI	GGNCC	7 cut(s)	440, 726, 1544, 1571, 2473, 2666, 2707
PspPPI	RGGWCCY	1 cut(s)	440
PstI	CTGCAG	2 cut(s)	2058, 2635
PstNI	CAGNNNCTG	3 cut(s)	35, 1238, 2887
PsuI	RGATCY	3 cut(s)	316, 2011, 2442
PvuII	CAGCTG	2 cut(s)	752, 2572
RsaI	GTAC	8 cut(s)	672, 924, 1107, 1582, 2217, 2407, 2568, 2752
RsaNI	GTAC	8 cut(s)	671, 923, 1106, 1581, 2216, 2406, 2567, 2751
RseI	CAYNNNNRTG	3 cut(s)	1174, 2367, 2799
SacI	GAGCTC	1 cut(s)	1036
SaqAI	TTAA	9 cut(s)	216, 269, 684, 840, 885, 1188, 1278, 1940, 2778
Sau96I	GGNCC	7 cut(s)	440, 726, 1544, 1571, 2473, 2666, 2707
SchI	GAGTC	8 cut(s)	22, 233, 302, 518, 1430, 1741, 1861, 2444
ScrFI	CCNGG	8 cut(s)	483, 826, 1197, 1233, 1311, 1353, 1548, 1713
SduI	GDGCHC	7 cut(s)	184, 203, 833, 1036, 1603, 1719, 2794
SfaNI	GCATC	9 cut(s)	86, 835, 1084, 1466, 1611, 2304, 2371, 2600, 2617
SfcI	CTRYAG	4 cut(s)	636, 1882, 2054, 2631
Sfr274I	CTCGAG	1 cut(s)	1763
SinI	GGWCC	4 cut(s)	440, 1571, 2473, 2707
SlaI	CTCGAG	1 cut(s)	1763
SmiMI	CAYNNNNRTG	3 cut(s)	1174, 2367, 2799
SmlI	CTYRAG	3 cut(s)	658, 1026, 1763
SmoI	CTYRAG	3 cut(s)	658, 1026, 1763
SpeI	ACTAGT	1 cut(s)	272
SseBI	AGGCCT	1 cut(s)	2108
SsiI	CCGC	4 cut(s)	932, 1091, 1295, 1789
SspMI	CTAG	5 cut(s)	264, 273, 723, 1461, 1503
SstI	GAGCTC	1 cut(s)	1036
StuI	AGGCCT	1 cut(s)	2108
StyD4I	CCNGG	8 cut(s)	481, 824, 1195, 1231, 1309, 1351, 1546, 1711
StyI	CCWWGG	1 cut(s)	722
TaaI	ACNGT	8 cut(s)	341, 455, 760, 927, 1264, 1883, 2646, 2759
TaiI	ACGT	3 cut(s)	403, 748, 2136
TaqI	TCGA	6 cut(s)	169, 397, 1285, 1764, 1855, 2842
TatI	WGTACW	5 cut(s)	922, 2215, 2405, 2566, 2750
TauI	GCSGC	2 cut(s)	934, 1791
TfiI	GAWTC	8 cut(s)	358, 394, 614, 866, 916, 1969, 2156, 2884
Tru1I	TTAA	9 cut(s)	216, 269, 684, 840, 885, 1188, 1278, 1940, 2778
Tru9I	TTAA	9 cut(s)	216, 269, 684, 840, 885, 1188, 1278, 1940, 2778
TscAI	CASTG	5 cut(s)	460, 765, 932, 1043, 2134
TseFI	GTSAC	2 cut(s)	2129, 2252
Tsp45I	GTSAC	2 cut(s)	2129, 2252
TspDTI	ATGAA	9 cut(s)	128, 456, 624, 753, 792, 890, 1727, 2541, 2717
TspGWI	ACGGA	1 cut(s)	1164
TspRI	CASTG	5 cut(s)	460, 765, 932, 1043, 2134
Van91I	CCANNNNNTGG	2 cut(s)	1214, 1610
VneI	GTGCAC	2 cut(s)	180, 1599
VpaK11BI	GGWCC	4 cut(s)	440, 1571, 2473, 2707
VspI	ATTAAT	3 cut(s)	216, 1940, 2778
XapI	RAATTY	6 cut(s)	1082, 1552, 1749, 1829, 2037, 2585
XbaI	TCTAGA	1 cut(s)	1460
XceI	RCATGY	4 cut(s)	901, 1931, 2237, 2504
XcmI	CCANNNNNNNNNTGG	1 cut(s)	239
XhoI	CTCGAG	1 cut(s)	1763
XmaJI	CCTAGG	1 cut(s)	722
XmiI	GTMKAC	1 cut(s)	626
XmnI	GAANNNNTTC	2 cut(s)	950, 1303
XspI	CTAG	5 cut(s)	264, 273, 723, 1461, 1503
ZraI	GACGTC	1 cut(s)	401
Zsp2I	ATGCAT	1 cut(s)	2364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.