Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007034

Physical Location & Seq

Genomic Coordinates Reverse (-)

25725152 .. 25732562

Gene Structure 7411 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1245500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2976 bp

ATGGCGACAGAAAGTCCAATAAGAATGTCGGAAACTAGTGGAAAATGGGCTCCTCGCAAGAAGACTGCAAAACTTGCACCTTCATCTGCTAATATGGCAGCAGAGGAGTTGAAATTGCTTCTTAGGGGCCATAAGTTTCAAAGCAGCGGACAGGATGTTTCCCCCAATCGAAGTGGAAGTGCACCCCCAAGTATGGAGGGTTCGTTTTTGGCCATCGATAACCTGCTGTCCCAGCAGGACTCGAGCATGAATGGAAGCTTGGCAACTCTAGGTAGTGTTATTGAAAGGTGTGAGACTGAGGAACAATTACGGGCTGATCCAGCTTATTTGGCATATTACTGTGCCAATGTCAATTTGAATCCTAGACTTCCTCGTCCACTTATTTCATGGGAGAATCGACGTCTGGCACGCAATATTGGTAGTTTCAGCCAGAGTCAGGGACCTGTAGATGGGAGTGGCAATTTTCTGTTGCATATGTCTCAAGGATCACTTCCTACACACAAGGAAGAATCTGAGGATGATCAGTCTTCCCAACTCTTTTCTAATGATTGGGTAGATCAGACAAGTGAAGTTTGGTTGGGAGAAGGTGCTGCGTCATTGGTAGGCCAACACCAAAATGTGGGTGATTTAATACAGGAAGATTTTGGTTGCTCTTCACCACCTGTCCCTAATCACTCTCATACATTAGGCAAGGAAGTACCTGAGGAATTCAATGATCACAGTACCGATTCCAGTTCCATGCATGATCCTGCTATTAATGTGACAACTACCATTAAGCCCAGCGTGGATGCAGAAACTGCTGATGCTACAGCGCTTTCACCAAATGACGATTCCTGGCCCGCCTCTGAAATTTCCAGGAGCACAGCTGTTAATGATGCAAGTGTTGCGGTCATTGAATCTGAAATGAAGGCTCTTAACATATCGGACTTGATAGAGAATCAAAAGAAACAAGAACAATGGGAGCGCAGTTGCCAGAATCATTTCTTGCAGCAGCATCAGCAACAAAACAATTTTTTTCAAGTTCAAAGTGGCAAGTCGCAAATAACCTCTCAAGGAACTGACGGTACATATGTTAGCATGGATCAGTATCTTCATAATACATCCAAGTATGCAGCGGATGTACAACCAGTTCATCAGACATCTGGGTTCACACCTCCACTATATGCAACTGCAGCCTACTTGAATTCAGCTAATCCATATTACTCAAATATTCAAGGACCGGGCTTTCTCACTCCACCATATCTTAGTGGATATACTTTGAATCCCACTGCCTTTTCTCCATATATTGGTGGATACCATCCTCCGGGTGCTGTTCCTGTGGTTGTTGATGGAACTGTGGGTCCAAGCTTTAATGCTCGAACATCTGGGGTTGCAACTGGGGGCATCCTCTCACCTGGTGCTGATATGCAACATTCAAGCAAGTTCTATGGTCAACTTGGATATCCGCTGCAAACTTCTTTTTCTGAACCTATGTACATGCAATACCACCAGCAGCCTTTTGTGGACTCGTATGGTGGTCCAAGACAATTTGATCCATTGGCATCAAGAGGTGGTGTTGACTCAAAGAAAGTGTCCAGTCACGGTTCTTATTTGGATGAACACAAAAGCCAACATCAGAGAATTGGAAACATGGGGAATTTGAACCCACAAAGAGGAGGGTCAATGACTCCTAACTATCTTGGAAGTGCACCAAATGTTGGTATTTTGATGCAGTATCCAACCCCTCCCCTTACTAGTCCAGTTTCGCCAGTACCCCTCATAGGTTCAGGTATAAATACTGGCTTGTATCCTGCATGGCCAAGGCACAGGGGCTTTGAGAGTTATGACGACCCCAAGATTTATAATTTCCTTGAAGAGTTGAAATCTGGCAAAGGCCGCAGATTTGAGTTATCTGATATCACAGGACATATTGTTGAATTCAGTGCTGATCAACATGGGAGTCGATTTATTCAGCAGAAGTTGGAAAACTGTAGTGTTGAAGAGAAGGCTTCTGTATTCAAAGAAGTTCTCCCACATGCTTCTAAACTAATGACTGATGTTTTTGGTAACTATGTGATTCAGAAGTTTTTTGAGTATGGAACCCCTCAGCAGAGAAAAGAACTTGCCAATCAACTTTCAGGTCAAATTTTGCCTTTAAGTTTGCAGATGTATGGCTGTCGTGTAATCCAAAAGGCACTTGAGGTTATTGAACTTGAACAGAAAGTGCAGCTTGTACATGAGCTGGATGGGCATGTTATGAGATGCGTTCGTGATCAAAATGGAAACCATGTCATACAAAAGTGCATTGAGAGCATTCCAACAGAAAAAATTAGGTTTATAATCTCTGCTTTCCGTGGTCAAGTTGCAACACTTTCAATCCATCCTTATGGCTGTCGTGTTATACAGAGAGTTCTGGAACATTGTACAGATGAGCTGCAATGCCAGTTCATAGTTGATGAGATCTTGGAGTCTGTTGCTGGTCTTGCCCAGGACCAGTATGGCAACTATGTCACACAGCATGTATTGGAGAGGGGAAAACCTAATGAAAGAAGGAAAATTATTAACAAGTTGTCGGGTCATTTCGTACCTCTGAGCCAGCACAAGTTTGCTTCAAATGTTGTTGAGAAATGTTTGGAATATGGTGGTGCTGCAGAACGAGAACTATTAATTAGGGAGATCGTTGGGCATAATGAAGGAAATGATAATTTGTTGGTAATGATGAAGGACCAATATGCAAATTATGTGGTGCAGAAGACCCTTGAAATCTGTAATGATAGTCAACGGGTGTTATTAATTAATCGTATAAGAGCTCACACAAACGCATTGAAGAAGTACACTTATGGGAAGCACATCGTAGCACGGTTTGAGCAGCTATTTGGAGAAGGTAAAATCTATCAGGATCGTGATGGGGACGAAGGCAAAATATCCAAGAAAGTACCTTCAGTGAATCTGTGGTTTGTCAATGCATACAGGGAATGTGTATCCCATGGAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632 NABP PUF

Protein Analysis

992

Amino Acids

109.86

Weight (kDa)

5.95

Isoelectric Point (pI)

44.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	636 - 670	6.8e-11	Pumilio-family RNA binding repeat
PUF	PF00806	674 - 705	1.2e-08	Pumilio-family RNA binding repeat
PUF	PF00806	708 - 741	6.3e-09	Pumilio-family RNA binding repeat
PUF_NOP9	PF22493	743 - 826	2.2e-06	NOP9-like PUF repeat domain
PUF	PF00806	780 - 815	3.1e-13	Pumilio-family RNA binding repeat
PUF	PF00806	818 - 850	1.5e-07	Pumilio-family RNA binding repeat
PUF	PF00806	853 - 886	2.7e-09	Pumilio-family RNA binding repeat
PUF	PF00806	898 - 928	3.8e-07	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25880 AT4G25880 AT4G25880 AT4G25880 AT4G25880
fragaria_vesca	FvH4_4g07600 FvH4_4g07600 FvH4_4g07600
malus_domestica	MD13G1245500.v1.1 MD16G1252800.v1.1
prunus_persica	Prupe.1G079300_v2.0.a1 Prupe.1G079300_v2.0.a1
pyrus_communis	pycom13g21640 pycom16g21410
rosa_chinensis	RchiOBHm_Chr4g0400221
rosa_laevigata	RLG00000009203
rosa_multiflora	Rmu_sc0004338.1_g000002
rosa_roxburghii	Rroxscaffold_5G00345180
rosa_rugosa	Rorug04G0023900 Rorug04G0024000
rosa_samantha	Rh4AG098900 Rh4BG097200 Rh4CG108400 Rh4DG093900
rosa_wichuraiana	Rw4G008220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1842, 2318
AasI	GACNNNNNNGTC	1 cut(s)	372
AatII	GACGTC	1 cut(s)	403
Acc36I	ACCTGC	1 cut(s)	231
AccB7I	CCANNNNNTGG	3 cut(s)	619, 1286, 1697
AciI	CCGC	6 cut(s)	147, 840, 887, 1115, 1445, 1876
AclWI	GGATC	6 cut(s)	311, 493, 740, 1089, 1526, 2886
AcoI	YGGCCR	2 cut(s)	210, 1796
AcsI	RAATTY	6 cut(s)	707, 849, 1183, 1636, 1916, 2124
AcuI	CTGAAG	1 cut(s)	2904
AcyI	GRCGYC	1 cut(s)	400
AdeI	CACNNNGTG	1 cut(s)	1397
AfeI	AGCGCT	1 cut(s)	813
AfiI	CCNNNNNNNGG	9 cut(s)	193, 436, 449, 619, 1286, 1303, 1652, 1697, 1760
AhdI	GACNNNNNGTC	1 cut(s)	12
AhlI	ACTAGT	2 cut(s)	35, 1733
AjnI	CCWGG	4 cut(s)	833, 854, 1393, 2466
AjuI	GAANNNNNNNTTGG	4 cut(s)	242, 274, 814, 846
AloI	GAACNNNNNNTCC	2 cut(s)	1324, 1356
Alw21I	GWGCWC	4 cut(s)	184, 863, 1690, 2791
Alw26I	GTCTC	2 cut(s)	287, 483
Alw44I	GTGCAC	2 cut(s)	180, 1686
AlwI	GGATC	6 cut(s)	311, 493, 740, 1089, 1526, 2886
AlwNI	CAGNNNCTG	1 cut(s)	797
Ama87I	CYCGRG	1 cut(s)	241
Aor51HI	AGCGCT	1 cut(s)	813
AoxI	GGCC	6 cut(s)	127, 210, 604, 836, 1796, 1873
ApaLI	GTGCAC	2 cut(s)	180, 1686
ApoI	RAATTY	6 cut(s)	707, 849, 1183, 1636, 1916, 2124
ArsI	GACNNNNNNTTYG	2 cut(s)	1556, 1588
AseI	ATTAAT	4 cut(s)	756, 2645, 2771, 2775
Asp700I	GAANNNNTTC	1 cut(s)	980
AspLEI	GCGC	2 cut(s)	814, 966
AspS9I	GGNCC	8 cut(s)	127, 440, 837, 1217, 1340, 1517, 2470, 2704
AsuC2I	CCSGG	2 cut(s)	1221, 1305
AsuHPI	GGTGA	4 cut(s)	635, 648, 810, 1383
AvaI	CYCGRG	1 cut(s)	241
AvaII	GGWCC	6 cut(s)	440, 1217, 1340, 1517, 2470, 2704
AxyI	CCTNAGG	1 cut(s)	702
BaeGI	GKGCMC	2 cut(s)	184, 1690
BaeI	ACNNNNGTAYC	2 cut(s)	1056, 1089
BalI	TGGCCA	2 cut(s)	212, 1798
BanII	GRGCYC	2 cut(s)	52, 2791
BbsI	GAAGAC	3 cut(s)	68, 519, 2738
Bbv12I	GWGCWC	4 cut(s)	184, 863, 1690, 2791
BbvCI	CCTCAGC	1 cut(s)	2085
BccI	CCATC	7 cut(s)	221, 443, 1305, 1322, 2219, 2367, 2879
BciT130I	CCWGG	4 cut(s)	835, 856, 1395, 2468
BciVI	GTATCC	4 cut(s)	1286, 1725, 1797, 2971
BclI	TGATCA	4 cut(s)	520, 715, 1927, 2251
BcnI	CCSGG	2 cut(s)	1221, 1305
BcoDI	GTCTC	2 cut(s)	287, 483
BcuI	ACTAGT	2 cut(s)	35, 1733
BfaI	CTAG	4 cut(s)	36, 269, 363, 1734
BfmI	CTRYAG	5 cut(s)	444, 807, 1170, 1969, 2628
BfoI	RGCGCY	1 cut(s)	815
BfuAI	ACCTGC	1 cut(s)	231
BfuI	GTATCC	4 cut(s)	1286, 1725, 1797, 2971
BglII	AGATCT	1 cut(s)	2439
Bme1390I	CCNGG	6 cut(s)	835, 856, 1221, 1305, 1395, 2468
Bme18I	GGWCC	6 cut(s)	440, 1217, 1340, 1517, 2470, 2704
BmeRI	GACNNNNNGTC	1 cut(s)	12
BmeT110I	CYCGRG	1 cut(s)	241
BmgT120I	GGNCC	8 cut(s)	127, 440, 837, 1217, 1340, 1517, 2470, 2704
BmiI	GGNNCC	5 cut(s)	51, 128, 441, 1341, 2080
BmrFI	CCNGG	6 cut(s)	835, 856, 1221, 1305, 1395, 2468
BmrI	ACTGGG	1 cut(s)	1386
BmsI	GCATC	8 cut(s)	778, 793, 865, 1003, 1392, 1550, 1698, 2231
BmuI	ACTGGG	1 cut(s)	1386
BpiI	GAAGAC	3 cut(s)	68, 519, 2738
Bpu10I	CCTNAGC	1 cut(s)	2085
BpuEI	CTTGAG	3 cut(s)	465, 1035, 2198
BpuMI	CCSGG	2 cut(s)	1221, 1305
Bsa29I	ATCGAT	1 cut(s)	216
BsaBI	GATNNNNATC	1 cut(s)	1086
BsaHI	GRCGYC	1 cut(s)	400
BsaJI	CCNNGG	4 cut(s)	1799, 2332, 2466, 2965
BsaXI	ACNNNNNCTCC	2 cut(s)	188, 218
Bsc4I	CCNNNNNNNGG	9 cut(s)	193, 436, 449, 619, 1286, 1303, 1652, 1697, 1760
Bse1I	ACTGG	9 cut(s)	732, 1127, 1381, 1575, 1739, 1748, 1783, 2422, 2473
Bse21I	CCTNAGG	1 cut(s)	702
Bse3DI	GCAATG	1 cut(s)	2423
Bse8I	GATNNNNATC	1 cut(s)	1086
BseBI	CCWGG	4 cut(s)	835, 856, 1395, 2468
BseCI	ATCGAT	1 cut(s)	216
BseDI	CCNNGG	4 cut(s)	1799, 2332, 2466, 2965
BseJI	GATNNNNATC	1 cut(s)	1086
BseLI	CCNNNNNNNGG	9 cut(s)	193, 436, 449, 619, 1286, 1303, 1652, 1697, 1760
BseMI	GCAATG	1 cut(s)	2423
BseMII	CTCAG	5 cut(s)	288, 504, 693, 2099, 2561
BseNI	ACTGG	9 cut(s)	732, 1127, 1381, 1575, 1739, 1748, 1783, 2422, 2473
BseRI	GAGGAG	3 cut(s)	42, 119, 1668
BseSI	GKGCMC	2 cut(s)	184, 1690
BseYI	CCCAGC	2 cut(s)	231, 779
BsgI	GTGCAG	2 cut(s)	2225, 2747
BshFI	GGCC	6 cut(s)	129, 212, 606, 838, 1798, 1875
BshVI	ATCGAT	1 cut(s)	216
BsiHKAI	GWGCWC	4 cut(s)	184, 863, 1690, 2791
BsiHKCI	CYCGRG	1 cut(s)	241
BsiSI	CCGG	2 cut(s)	1220, 1304
BslFI	GGGAC	4 cut(s)	214, 453, 650, 2903
BslI	CCNNNNNNNGG	9 cut(s)	193, 436, 449, 619, 1286, 1303, 1652, 1697, 1760
BsmAI	GTCTC	2 cut(s)	287, 483
BsmFI	GGGAC	4 cut(s)	214, 453, 650, 2903
BsmI	GAATGC	1 cut(s)	2292
BsnI	GGCC	6 cut(s)	129, 212, 606, 838, 1798, 1875
BsoBI	CYCGRG	1 cut(s)	241
Bsp1286I	GDGCHC	5 cut(s)	52, 184, 863, 1690, 2791
Bsp1407I	TGTACA	4 cut(s)	1120, 1473, 2212, 2402
Bsp19I	CCATGG	1 cut(s)	2965
BspACI	CCGC	6 cut(s)	147, 840, 887, 1115, 1445, 1876
BspANI	GGCC	6 cut(s)	129, 212, 606, 838, 1798, 1875
BspCNI	CTCAG	5 cut(s)	289, 505, 694, 2098, 2562
BspDI	ATCGAT	1 cut(s)	216
BspLI	GGNNCC	5 cut(s)	51, 128, 441, 1341, 2080
BspMAI	CTGCAG	2 cut(s)	1174, 2632
BspMI	ACCTGC	1 cut(s)	231
BspPI	GGATC	6 cut(s)	311, 493, 740, 1089, 1526, 2886
BspQI	GCTCTTC	1 cut(s)	658
BsrDI	GCAATG	1 cut(s)	2423
BsrGI	TGTACA	4 cut(s)	1120, 1473, 2212, 2402
BsrI	ACTGG	9 cut(s)	732, 1127, 1381, 1575, 1739, 1748, 1783, 2422, 2473
BssECI	CCNNGG	4 cut(s)	1799, 2332, 2466, 2965
BssNI	GRCGYC	1 cut(s)	400
BssT1I	CCWWGG	2 cut(s)	1799, 2965
Bst2UI	CCWGG	4 cut(s)	835, 856, 1395, 2468
Bst4CI	ACNGT	7 cut(s)	341, 722, 1064, 1336, 1583, 1970, 2841
Bst6I	CTCTTC	3 cut(s)	658, 1848, 1974
BstACI	GRCGYC	1 cut(s)	400
BstAPI	GCANNNNNTGC	3 cut(s)	74, 797, 884
BstAUI	TGTACA	4 cut(s)	1120, 1473, 2212, 2402
BstC8I	GCNNGC	3 cut(s)	409, 840, 2576
BstDEI	CTNAG	7 cut(s)	122, 297, 513, 702, 1244, 2085, 2570
BstDSI	CCRYGG	2 cut(s)	2332, 2965
BstH2I	RGCGCY	1 cut(s)	815
BstHHI	GCGC	2 cut(s)	814, 966
BstMAI	GTCTC	2 cut(s)	287, 483
BstNI	CCWGG	4 cut(s)	835, 856, 1395, 2468
BstNSI	RCATGY	4 cut(s)	1480, 2018, 2234, 2501
BstSCI	CCNGG	6 cut(s)	833, 854, 1219, 1303, 1393, 2466
BstSFI	CTRYAG	5 cut(s)	444, 807, 1170, 1969, 2628
BstSLI	GKGCMC	2 cut(s)	184, 1690
BstV2I	GAAGAC	3 cut(s)	68, 519, 2738
BstX2I	RGATCY	1 cut(s)	2439
BstXI	CCANNNNNNTGG	1 cut(s)	2366
BstYI	RGATCY	1 cut(s)	2439
Bsu15I	ATCGAT	1 cut(s)	216
Bsu36I	CCTNAGG	1 cut(s)	702
BsuI	GTATCC	4 cut(s)	1286, 1725, 1797, 2971
BsuRI	GGCC	6 cut(s)	129, 212, 606, 838, 1798, 1875
BsuTUI	ATCGAT	1 cut(s)	216
BtgI	CCRYGG	2 cut(s)	2332, 2965
BtsI	GCAGTG	1 cut(s)	1266
BtsIMutI	CAGTG	3 cut(s)	1266, 1927, 2928
BveI	ACCTGC	1 cut(s)	231
Cac8I	GCNNGC	3 cut(s)	409, 840, 2576
CaiI	CAGNNNCTG	1 cut(s)	797
CfoI	GCGC	2 cut(s)	814, 966
Cfr13I	GGNCC	8 cut(s)	127, 440, 837, 1217, 1340, 1517, 2470, 2704
ClaI	ATCGAT	1 cut(s)	216
CseI	GACGC	1 cut(s)	582
CsiI	ACCWGGT	1 cut(s)	1393
CspCI	CAANNNNNGTGG	4 cut(s)	154, 189, 2703, 2738
DdeI	CTNAG	7 cut(s)	122, 297, 513, 702, 1244, 2085, 2570
DraIII	CACNNNGTG	1 cut(s)	1397
DrdI	GACNNNNNNGTC	1 cut(s)	372
DriI	GACNNNNNGTC	1 cut(s)	12
DseDI	GACNNNNNNGTC	1 cut(s)	372
EaeI	YGGCCR	2 cut(s)	210, 1796
Eam1104I	CTCTTC	3 cut(s)	658, 1848, 1974
Eam1105I	GACNNNNNGTC	1 cut(s)	12
EarI	CTCTTC	3 cut(s)	658, 1848, 1974
Ecl136II	GAGCTC	1 cut(s)	2789
Eco130I	CCWWGG	2 cut(s)	1799, 2965
Eco24I	GRGCYC	2 cut(s)	52, 2791
Eco32I	GATATC	2 cut(s)	1442, 1897
Eco47I	GGWCC	6 cut(s)	440, 1217, 1340, 1517, 2470, 2704
Eco47III	AGCGCT	1 cut(s)	813
Eco53kI	GAGCTC	1 cut(s)	2789
Eco57I	CTGAAG	1 cut(s)	2904
Eco81I	CCTNAGG	1 cut(s)	702
Eco88I	CYCGRG	1 cut(s)	241
EcoICRI	GAGCTC	1 cut(s)	2789
EcoO109I	RGGNCCY	1 cut(s)	440
EcoRI	GAATTC	3 cut(s)	707, 1183, 1916
EcoRII	CCWGG	4 cut(s)	833, 854, 1393, 2466
EcoRV	GATATC	2 cut(s)	1442, 1897
EcoT14I	CCWWGG	2 cut(s)	1799, 2965
EcoT22I	ATGCAT	2 cut(s)	744, 2947
EcoT38I	GRGCYC	2 cut(s)	52, 2791
ErhI	CCWWGG	2 cut(s)	1799, 2965
FalI	AAGNNNNNCTT	4 cut(s)	474, 506, 2193, 2225
FaqI	GGGAC	4 cut(s)	214, 453, 650, 2903
FauI	CCCGC	1 cut(s)	847
FauNDI	CATATG	2 cut(s)	474, 1069
FbaI	TGATCA	4 cut(s)	520, 715, 1927, 2251
FriOI	GRGCYC	2 cut(s)	52, 2791
FspBI	CTAG	4 cut(s)	36, 269, 363, 1734
GlaI	GCGC	2 cut(s)	813, 965
GsaI	CCCAGC	2 cut(s)	235, 783
HaeII	RGCGCY	1 cut(s)	815
HaeIII	GGCC	6 cut(s)	129, 212, 606, 838, 1798, 1875
HapII	CCGG	2 cut(s)	1220, 1304
HgaI	GACGC	1 cut(s)	582
HhaI	GCGC	2 cut(s)	814, 966
Hin1I	GRCGYC	1 cut(s)	400
Hin6I	GCGC	2 cut(s)	812, 964
HinP1I	GCGC	2 cut(s)	812, 964
HincII	GTYRAC	3 cut(s)	1433, 1558, 2759
HindII	GTYRAC	3 cut(s)	1433, 1558, 2759
HindIII	AAGCTT	2 cut(s)	256, 1345
HpaII	CCGG	2 cut(s)	1220, 1304
HphI	GGTGA	4 cut(s)	635, 648, 810, 1383
Hpy166II	GTNNAC	9 cut(s)	182, 377, 1149, 1433, 1504, 1558, 1688, 2759, 2814
Hpy188III	TCNNGA	5 cut(s)	1545, 2249, 2393, 2876, 2882
Hpy8I	GTNNAC	9 cut(s)	182, 377, 1149, 1433, 1504, 1558, 1688, 2759, 2814
Hpy99I	CGWCG	1 cut(s)	402
HpyCH4III	ACNGT	7 cut(s)	341, 722, 1064, 1336, 1583, 1970, 2841
HpyCH4IV	ACGT	1 cut(s)	400
HpyF3I	CTNAG	7 cut(s)	122, 297, 513, 702, 1244, 2085, 2570
HpySE526I	ACGT	1 cut(s)	400
Hsp92I	GRCGYC	1 cut(s)	400
HspAI	GCGC	2 cut(s)	812, 964
Ksp22I	TGATCA	4 cut(s)	520, 715, 1927, 2251
LguI	GCTCTTC	1 cut(s)	658
LmnI	GCTCC	3 cut(s)	55, 858, 961
LweI	GCATC	8 cut(s)	778, 793, 865, 1003, 1392, 1550, 1698, 2231
MabI	ACCWGGT	1 cut(s)	1393
MaeI	CTAG	4 cut(s)	36, 269, 363, 1734
MaeII	ACGT	1 cut(s)	400
MaeIII	GTNAC	4 cut(s)	760, 1577, 2045, 2488
MflI	RGATCY	1 cut(s)	2439
MhlI	GDGCHC	5 cut(s)	52, 184, 863, 1690, 2791
MlsI	TGGCCA	2 cut(s)	212, 1798
MluNI	TGGCCA	2 cut(s)	212, 1798
MlyI	GAGTC	7 cut(s)	233, 442, 1499, 1553, 1660, 1948, 2456
MmeI	TCCRAC	4 cut(s)	9, 1742, 1941, 2321
Mox20I	TGGCCA	2 cut(s)	212, 1798
Mph1103I	ATGCAT	2 cut(s)	744, 2947
MroXI	GAANNNNTTC	1 cut(s)	980
MscI	TGGCCA	2 cut(s)	212, 1798
MslI	CAYNNNNRTG	2 cut(s)	615, 2364
Msp20I	TGGCCA	2 cut(s)	212, 1798
MspA1I	CMGCKG	4 cut(s)	147, 866, 1115, 1447
MspI	CCGG	2 cut(s)	1220, 1304
MspR9I	CCNGG	6 cut(s)	835, 856, 1221, 1305, 1395, 2468
Mva1269I	GAATGC	1 cut(s)	2292
MvaI	CCWGG	4 cut(s)	835, 856, 1395, 2468
NciI	CCSGG	2 cut(s)	1221, 1305
NcoI	CCATGG	1 cut(s)	2965
NdeI	CATATG	2 cut(s)	474, 1069
NlaIV	GGNNCC	5 cut(s)	51, 128, 441, 1341, 2080
NmuCI	GTSAC	3 cut(s)	760, 1577, 2488
NsiI	ATGCAT	2 cut(s)	744, 2947
NspI	RCATGY	4 cut(s)	1480, 2018, 2234, 2501
PacI	TTAATTAA	1 cut(s)	2775
PaeR7I	CTCGAG	1 cut(s)	241
PciSI	GCTCTTC	1 cut(s)	658
PctI	GAATGC	1 cut(s)	2292
PdmI	GAANNNNTTC	1 cut(s)	980
PflMI	CCANNNNNTGG	3 cut(s)	619, 1286, 1697
PfoI	TCCNGGA	1 cut(s)	854
PleI	GAGTC	7 cut(s)	233, 441, 1499, 1553, 1660, 1947, 2455
PpsI	GAGTC	7 cut(s)	233, 441, 1499, 1553, 1660, 1947, 2455
PpuMI	RGGWCCY	1 cut(s)	440
PshBI	ATTAAT	4 cut(s)	756, 2645, 2771, 2775
PsiI	TTATAA	2 cut(s)	1842, 2318
Psp124BI	GAGCTC	1 cut(s)	2791
Psp5II	RGGWCCY	1 cut(s)	440
Psp6I	CCWGG	4 cut(s)	833, 854, 1393, 2466
PspFI	CCCAGC	2 cut(s)	231, 779
PspGI	CCWGG	4 cut(s)	833, 854, 1393, 2466
PspN4I	GGNNCC	5 cut(s)	51, 128, 441, 1341, 2080
PspPI	GGNCC	8 cut(s)	127, 440, 837, 1217, 1340, 1517, 2470, 2704
PspPPI	RGGWCCY	1 cut(s)	440
PspXI	VCTCGAGB	1 cut(s)	241
PsrI	GAACNNNNNNTAC	6 cut(s)	184, 216, 1048, 1080, 1113, 1145
PstI	CTGCAG	2 cut(s)	1174, 2632
PstNI	CAGNNNCTG	1 cut(s)	797
PsuI	RGATCY	1 cut(s)	2439
PvuII	CAGCTG	1 cut(s)	866
RseI	CAYNNNNRTG	2 cut(s)	615, 2364
SacI	GAGCTC	1 cut(s)	2791
SapI	GCTCTTC	1 cut(s)	658
Sau96I	GGNCC	8 cut(s)	127, 440, 837, 1217, 1340, 1517, 2470, 2704
SchI	GAGTC	7 cut(s)	233, 442, 1499, 1553, 1660, 1948, 2456
ScrFI	CCNGG	6 cut(s)	835, 856, 1221, 1305, 1395, 2468
SduI	GDGCHC	5 cut(s)	52, 184, 863, 1690, 2791
SexAI	ACCWGGT	1 cut(s)	1393
SfaNI	GCATC	8 cut(s)	778, 793, 865, 1003, 1392, 1550, 1698, 2231
SfcI	CTRYAG	5 cut(s)	444, 807, 1170, 1969, 2628
Sfr274I	CTCGAG	1 cut(s)	241
SinI	GGWCC	6 cut(s)	440, 1217, 1340, 1517, 2470, 2704
SlaI	CTCGAG	1 cut(s)	241
SmiMI	CAYNNNNRTG	2 cut(s)	615, 2364
SmlI	CTYRAG	4 cut(s)	241, 480, 1050, 2177
SmoI	CTYRAG	4 cut(s)	241, 480, 1050, 2177
SpeI	ACTAGT	2 cut(s)	35, 1733
SsiI	CCGC	6 cut(s)	147, 840, 887, 1115, 1445, 1876
SspI	AATATT	2 cut(s)	415, 1210
SspMI	CTAG	4 cut(s)	36, 269, 363, 1734
SstI	GAGCTC	1 cut(s)	2791
StyD4I	CCNGG	6 cut(s)	833, 854, 1219, 1303, 1393, 2466
StyI	CCWWGG	2 cut(s)	1799, 2965
TaaI	ACNGT	7 cut(s)	341, 722, 1064, 1336, 1583, 1970, 2841
TaiI	ACGT	1 cut(s)	403
TaqI	TCGA	6 cut(s)	169, 216, 242, 397, 1357, 1942
TatI	WGTACW	5 cut(s)	1120, 1473, 2212, 2402, 2811
TauI	GCSGC	1 cut(s)	1878
TscAI	CASTG	3 cut(s)	1273, 1927, 2928
TseFI	GTSAC	3 cut(s)	760, 1577, 2488
Tsp45I	GTSAC	3 cut(s)	760, 1577, 2488
TspGWI	ACGGA	1 cut(s)	2321
TspRI	CASTG	3 cut(s)	1273, 1927, 2928
Van91I	CCANNNNNTGG	3 cut(s)	619, 1286, 1697
VneI	GTGCAC	2 cut(s)	180, 1686
VpaK11BI	GGWCC	6 cut(s)	440, 1217, 1340, 1517, 2470, 2704
VspI	ATTAAT	4 cut(s)	756, 2645, 2771, 2775
XapI	RAATTY	6 cut(s)	707, 849, 1183, 1636, 1916, 2124
XceI	RCATGY	4 cut(s)	1480, 2018, 2234, 2501
XcmI	CCANNNNNNNNNTGG	2 cut(s)	384, 2474
XhoI	CTCGAG	1 cut(s)	241
XmnI	GAANNNNTTC	1 cut(s)	980
XspI	CTAG	4 cut(s)	36, 269, 363, 1734
ZraI	GACGTC	1 cut(s)	401
Zsp2I	ATGCAT	2 cut(s)	744, 2947

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.