Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0007034

Physical Location & Seq

Genomic Coordinates Reverse (-)

48763746 .. 48769900

Gene Structure 6155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009203

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2847 bp

ATGGCGACTGAGAGTCCAATTAGAATGTCAGAAACTGGTGGAAACTGGGGTTCCCATAAGAAGCCGGCAAAACTTGCATCATCATCTGCTAATATGACAGCAGAGGAGTTGAAATTGCTTCTGAGAGACCATAGGTTTCATAGCAGCAGAAAGGATGTTGCCCCCAATCGAAGTGGAAGTGCACCTCCAAGTATGGAGGGCTCATTTTTGGCCATTAATAATCTGTTATCCCAGCAGGACTCTGGCATGACTGGCAGCTTGGCTAGTTTAACTAGTGTTATTGAAAGGTGCGAGTCTGAGGAGCAACTGCGTTCAGATCCAGCTTATCTGGCTTATTACTGTGCCAATGTCAATCTGAATCCAAGACTTCCTCCTCCTCTTATCTCATGGGAGAATCGACGTCTGGCACGCCATATCGGTAGTTTCAGTCCAAACTTGGGACCCGTTGATGACAGTGGTAATGGTTCATTGCATATGTCTCCAGGACTACTGCCTACACACGAGGAAGAGTCAGAGGATGATCAGACACCTCAACAGGCTTCTAATGATTGGGTAGATCAGACAAGTTCTGGAGAGGAAGCAGCTCAATTGATAGGACAACAGAAAAATGAAGATTCTTGTGGGTCTACTTCACCTGTAGATAATCATTCCCATACCTCAAGCAATGGGGTACCTGGGGAGTTTAATCAGAGTGCTGTTTCCAATTCCTTGCATGATCTCCCTAGGCCCAATGTAGACGAAGAACGTGCAGCTGATACAGTGCTTTCACTAAATGATGAAGCCTCATCCAGTCCTGTTGCAATTTCGTCGTCTCATGTTTATACCAGGAGCACAGGTGTTAATGATGCTGGTGTTTCCCTTGTTGAATCCGGAATGAAGTCTCTTAATGTATCAAACATGCCAGAAAATCAAAAGAATCAAGTACAGTGGCCGCCCAGCTACCAGAATAATTTCCTGCAATACCAGATACAGCAACAGCAAATCAATTTATGCCAAGTTCAAAATGCCACATCTCAGATAACTCCTCAAGGAGCTCACTGCACATATATTGGCATGGATCACTATCACCCTAATTCCTCCAAATTTGCAGCGGATGCACAGCCGGTACTCCAGACATCAGGGTTCACACATCCACTTTATGCAAACACAGCAGCCTATACGAGTGCAACACATCCATATTATTCCAATTTTCAGGCACAGAGCTTTGTTTCTCCACAATATGTAGGCGGATATGCTTTAAACCCCCCTGCTTTTTCTCCGTATGTTGGTGGATATCATCCTCCAGGGGCTGTTCCTGTTGTTGTTGAGGGAACTGTGGGTTCAAGCTTTAATGCTCGAACATCTGCGGGAAGCATTTCACCTGGGGCTGATCTGCAACATATAAGCAAGTCATATGGGCAGCCTGGGTTTCCTCTGCAAAGTTCTTATTCCGATCCTATGTATATGCAATACTATCAACAGCCGTTTGTGGAGTCATATGGTGTTTCTGGCCAGTATGCTCCATTGGCATCTAGAGGTGGTCTTGACTTGAACAAAGCATCCAATCATGCCACTAGTTTGGATGACCACAAAATCCAACATCCGAGGAATGGAGGGCCGGGGAATTTGAACTCACAAAGAGGACCCGTAAGTGCCAGCTATTATGGAAGTGCACCAAATGTTGGTGTTTTGATGCAGTATCCTACCTCACCCTTAAATAGTCCTGTTCTGCCAACATCATCCATAAGTTCAGGAAGAAATACTGGCTTATATCATGGATGGCCAGGGCACCGAGGCTTTGAGAGTCTTCATGACCCCAAAATTTATAATTTTCTTGAGGAGTTGAAATCTGGCAAAGGCCGCAGATTTGAGCTATCTGATATCACTGGACATATTGTTGAATTTAGTGCTGATCAACATGGGAGTCGATTTATCCAGCAGAAGTTGGAAAACTGTAGTATTGAAGAGAAGGCTTCTGTATTCAGAGAAGTTCTCCCACATGCTTGTCAATTAATGACTGATGTTTTTGGTAACTATGTGATTCAAAAGTTTTTTGAGTATGGAAGCCCTCAGCAAAGGAAGGATCTTGCCAATCAACTTTCAGGCCAAATTTTGCCTCTAAGTCTGCAGATGTATGGCTGTCGTGTAATCCAAAAGGCACTTGATGTGATTGAGCTTGAACAGAAAGCAAGGCTTGTACATGAGTTGGATGGACAAGTTATGAGATGTGTTCGTGACCAAAATGGAAACCATGTCATACAAAAGTGTATTGAGAGCATCCCAACAGAAAAGATTGGGTTTATAATATCTGCTTTCCATGGTCAAGTTGCACCACTTTCAATGCATCCCTATGGTTGCCGCGTTATACAGAGAGTTCTGGAGCATTGTACAAATGAGTTGCAATGCCAGTTTATAGTTGATGAGATCTTGGACTCTGTGTGTGCTCTTGCTCAGGACCAGTATGGCAATTATGTCTCCCAGCATGTATTGGAGAGGGGGAAACCTCATGAAAGAAGCCGAATTATTAGCAAGTTGTCGGGTCATATTGTACAGCTGAGCCAGCACAAATTTGCATCCAATGTTGTTGAAAAATGTTTGGAGTATGGTGATGCTGCAGAACGAGAACTGTTGATTAGGGAGATTGTTGGCCCTAATGATGGAAATGATAACTTATTGATAATGATGAAGGACCAATTTGCCAATTATGTGGTGCAGAAGGCTCTTGAAATCTGTACAGACAGTCAACGAACGATATTGATTAATCGCATAAGGGCTCATACTCATGCATTGAAGAAATACACTTATGGGAAACACATTGTTGCTCGATTTGAGCAGCTATTTGGAGAAGAAAATCAATCAGGATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632 NABP PUF

Protein Analysis

949

Amino Acids

104.25

Weight (kDa)

5.94

Isoelectric Point (pI)

48.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	624 - 658	1.9e-10	Pumilio-family RNA binding repeat
PUF	PF00806	660 - 693	3.4e-09	Pumilio-family RNA binding repeat
PUF	PF00806	696 - 729	4.6e-09	Pumilio-family RNA binding repeat
PUF	PF00806	768 - 803	3.3e-13	Pumilio-family RNA binding repeat
PUF	PF00806	806 - 838	3.7e-07	Pumilio-family RNA binding repeat
PUF	PF00806	841 - 874	6.8e-11	Pumilio-family RNA binding repeat
PUF	PF00806	887 - 916	4.7e-08	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25880 AT4G25880 AT4G25880 AT4G25880 AT4G25880
fragaria_vesca	FvH4_4g07600 FvH4_4g07600 FvH4_4g07600
malus_domestica	MD13G1245500.v1.1 MD16G1252800.v1.1
prunus_persica	Prupe.1G079300_v2.0.a1 Prupe.1G079300_v2.0.a1
pyrus_communis	pycom13g21640 pycom16g21410
rosa_chinensis	RchiOBHm_Chr4g0400221
rosa_laevigata	RLG00000009203
rosa_multiflora	Rmu_sc0004338.1_g000002
rosa_roxburghii	Rroxscaffold_5G00345180
rosa_rugosa	Rorug04G0023900 Rorug04G0024000
rosa_samantha	Rh4AG098900 Rh4BG097200 Rh4CG108400 Rh4DG093900
rosa_wichuraiana	Rw4G008220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1806, 2282
AatII	GACGTC	1 cut(s)	403
Acc65I	GGTACC	1 cut(s)	670
AccB1I	GGYRCC	2 cut(s)	670, 1767
AccB7I	CCANNNNNTGG	1 cut(s)	1661
AccI	GTMKAC	2 cut(s)	626, 735
AccII	CGCG	1 cut(s)	2340
AccIII	TCCGGA	1 cut(s)	869
AciI	CCGC	6 cut(s)	932, 1091, 1227, 1346, 1840, 2338
AclWI	GGATC	5 cut(s)	311, 1065, 1427, 2070, 2835
AcoI	YGGCCR	4 cut(s)	210, 929, 1489, 1760
AcsI	RAATTY	6 cut(s)	1082, 1603, 1800, 1880, 2088, 2546
AcyI	GRCGYC	1 cut(s)	400
AfaI	GTAC	7 cut(s)	672, 924, 1107, 2178, 2368, 2529, 2713
AfiI	CCNNNNNNNGG	5 cut(s)	437, 1265, 1589, 1661, 2636
AhdI	GACNNNNNGTC	1 cut(s)	12
AhlI	ACTAGT	2 cut(s)	272, 1553
AjnI	CCWGG	7 cut(s)	481, 673, 824, 1282, 1360, 1402, 1762
AloI	GAACNNNNNNTCC	2 cut(s)	1523, 1555
Alw21I	GWGCWC	5 cut(s)	184, 833, 1036, 1654, 2425
Alw26I	GTCTC	5 cut(s)	120, 483, 816, 885, 2458
Alw44I	GTGCAC	2 cut(s)	180, 1650
AlwI	GGATC	5 cut(s)	311, 1065, 1427, 2070, 2835
AlwNI	CAGNNNCTG	2 cut(s)	35, 1289
Aor13HI	TCCGGA	1 cut(s)	869
AoxI	GGCC	9 cut(s)	210, 725, 929, 1489, 1595, 1760, 1837, 2083, 2626
ApaLI	GTGCAC	2 cut(s)	180, 1650
ApeKI	GCWGC	9 cut(s)	144, 255, 581, 749, 1088, 1151, 1399, 2591, 2812
ApoI	RAATTY	6 cut(s)	1082, 1603, 1800, 1880, 2088, 2546
AseI	ATTAAT	3 cut(s)	216, 1991, 2739
Asp700I	GAANNNNTTC	2 cut(s)	950, 1354
Asp718I	GGTACC	1 cut(s)	670
AspA2I	CCTAGG	1 cut(s)	722
AspS9I	GGNCC	7 cut(s)	440, 726, 1595, 1622, 2434, 2627, 2668
AsuC2I	CCSGG	1 cut(s)	1599
AsuHPI	GGTGA	5 cut(s)	624, 1058, 1350, 1680, 2597
AvaII	GGWCC	4 cut(s)	440, 1622, 2434, 2668
AvrII	CCTAGG	1 cut(s)	722
BaeGI	GKGCMC	3 cut(s)	184, 1654, 1770
BalI	TGGCCA	3 cut(s)	212, 1491, 1762
BamHI	GGATCC	1 cut(s)	2840
BanI	GGYRCC	2 cut(s)	670, 1767
BanII	GRGCYC	3 cut(s)	203, 1036, 2755
BauI	CACGAG	1 cut(s)	500
BbsI	GAAGAC	1 cut(s)	1778
Bbv12I	GWGCWC	5 cut(s)	184, 833, 1036, 1654, 2425
BbvCI	CCTCAGC	1 cut(s)	2049
BbvI	GCAGC	9 cut(s)	156, 267, 593, 761, 1100, 1163, 1411, 2578, 2824
BccI	CCATC	3 cut(s)	1752, 2183, 2630
BceAI	ACGGC	1 cut(s)	1447
BcgI	CGANNNNNNTGC	4 cut(s)	728, 762, 789, 823
BciT130I	CCWGG	7 cut(s)	483, 675, 826, 1284, 1362, 1404, 1764
BciVI	GTATCC	1 cut(s)	1689
BclI	TGATCA	2 cut(s)	520, 1891
BcnI	CCSGG	1 cut(s)	1599
BcoDI	GTCTC	5 cut(s)	120, 483, 816, 885, 2458
BcuI	ACTAGT	2 cut(s)	272, 1553
BfaI	CTAG	5 cut(s)	264, 273, 723, 1512, 1554
BfmI	CTRYAG	4 cut(s)	636, 1933, 2105, 2592
BfuI	GTATCC	1 cut(s)	1689
BglII	AGATCT	1 cut(s)	2403
BlnI	CCTAGG	1 cut(s)	722
BlpI	GCTNAGC	1 cut(s)	2534
Bme1390I	CCNGG	8 cut(s)	483, 675, 826, 1284, 1362, 1404, 1599, 1764
Bme18I	GGWCC	4 cut(s)	440, 1622, 2434, 2668
BmeRI	GACNNNNNGTC	1 cut(s)	12
BmgT120I	GGNCC	7 cut(s)	440, 726, 1595, 1622, 2434, 2627, 2668
BmiI	GGNNCC	7 cut(s)	52, 441, 442, 672, 1624, 1769, 2842
BmrFI	CCNGG	8 cut(s)	483, 675, 826, 1284, 1362, 1404, 1599, 1764
BmrI	ACTGGG	1 cut(s)	55
BmuI	ACTGGG	1 cut(s)	55
BpiI	GAAGAC	1 cut(s)	1778
BplI	GAGNNNNNCTC	2 cut(s)	1956, 1988
BpmI	CTGGAG	5 cut(s)	465, 591, 1094, 1266, 2378
Bpu10I	CCTNAGC	2 cut(s)	2049, 2430
Bpu1102I	GCTNAGC	1 cut(s)	2534
BpuEI	CTTGAG	3 cut(s)	643, 1011, 1835
BpuMI	CCSGG	1 cut(s)	1599
BsaBI	GATNNNNATC	1 cut(s)	1062
BsaHI	GRCGYC	1 cut(s)	400
BsaI	GGTCTC	1 cut(s)	120
BsaWI	WCCGGW	1 cut(s)	869
BsaXI	ACNNNNNCTCC	2 cut(s)	820, 850
Bsc4I	CCNNNNNNNGG	5 cut(s)	437, 1265, 1589, 1661, 2636
Bse118I	RCCGGY	2 cut(s)	64, 1102
Bse1I	ACTGG	9 cut(s)	40, 50, 256, 789, 1492, 1747, 1870, 2386, 2437
Bse3DI	GCAATG	3 cut(s)	467, 670, 2387
Bse8I	GATNNNNATC	1 cut(s)	1062
BseAI	TCCGGA	1 cut(s)	869
BseBI	CCWGG	7 cut(s)	483, 675, 826, 1284, 1362, 1404, 1764
BseJI	GATNNNNATC	1 cut(s)	1062
BseLI	CCNNNNNNNGG	5 cut(s)	437, 1265, 1589, 1661, 2636
BseMI	GCAATG	3 cut(s)	467, 670, 2387
BseMII	CTCAG	6 cut(s)	113, 288, 1028, 2063, 2444, 2525
BseNI	ACTGG	9 cut(s)	40, 50, 256, 789, 1492, 1747, 1870, 2386, 2437
BseRI	GAGGAG	6 cut(s)	119, 314, 363, 366, 1014, 1832
BseSI	GKGCMC	3 cut(s)	184, 1654, 1770
BseXI	GCAGC	9 cut(s)	156, 267, 593, 761, 1100, 1163, 1411, 2578, 2824
BseYI	CCCAGC	3 cut(s)	231, 935, 2457
BsgI	GTGCAG	3 cut(s)	768, 1024, 2711
Bsh1236I	CGCG	1 cut(s)	2340
BshFI	GGCC	9 cut(s)	212, 727, 931, 1491, 1597, 1762, 1839, 2085, 2628
BshNI	GGYRCC	2 cut(s)	670, 1767
BsiHKAI	GWGCWC	5 cut(s)	184, 833, 1036, 1654, 2425
BsiSI	CCGG	4 cut(s)	65, 870, 1103, 1598
BslFI	GGGAC	1 cut(s)	453
BslI	CCNNNNNNNGG	5 cut(s)	437, 1265, 1589, 1661, 2636
BsmAI	GTCTC	5 cut(s)	120, 483, 816, 885, 2458
BsmBI	CGTCTC	1 cut(s)	816
BsmFI	GGGAC	1 cut(s)	453
BsnI	GGCC	9 cut(s)	212, 727, 931, 1491, 1597, 1762, 1839, 2085, 2628
Bso31I	GGTCTC	1 cut(s)	120
Bsp1286I	GDGCHC	8 cut(s)	184, 203, 833, 1036, 1654, 1770, 2425, 2755
Bsp13I	TCCGGA	1 cut(s)	869
Bsp1407I	TGTACA	4 cut(s)	2176, 2366, 2527, 2711
Bsp1720I	GCTNAGC	1 cut(s)	2534
Bsp19I	CCATGG	1 cut(s)	2296
BspACI	CCGC	6 cut(s)	932, 1091, 1227, 1346, 1840, 2338
BspANI	GGCC	9 cut(s)	212, 727, 931, 1491, 1597, 1762, 1839, 2085, 2628
BspCNI	CTCAG	6 cut(s)	114, 289, 1027, 2062, 2443, 2526
BspEI	TCCGGA	1 cut(s)	869
BspFNI	CGCG	1 cut(s)	2340
BspHI	TCATGA	2 cut(s)	1789, 2485
BspLI	GGNNCC	7 cut(s)	52, 441, 442, 672, 1624, 1769, 2842
BspMAI	CTGCAG	2 cut(s)	2109, 2596
BspPI	GGATC	5 cut(s)	311, 1065, 1427, 2070, 2835
BspT107I	GGYRCC	2 cut(s)	670, 1767
BspTNI	GGTCTC	1 cut(s)	120
BsrDI	GCAATG	3 cut(s)	467, 670, 2387
BsrFI	RCCGGY	2 cut(s)	64, 1102
BsrGI	TGTACA	4 cut(s)	2176, 2366, 2527, 2711
BsrI	ACTGG	9 cut(s)	40, 50, 256, 789, 1492, 1747, 1870, 2386, 2437
BssAI	RCCGGY	2 cut(s)	64, 1102
BssNI	GRCGYC	1 cut(s)	400
BssSI	CACGAG	1 cut(s)	500
BssT1I	CCWWGG	2 cut(s)	722, 2296
Bst2BI	CACGAG	1 cut(s)	500
Bst2UI	CCWGG	7 cut(s)	483, 675, 826, 1284, 1362, 1404, 1764
Bst4CI	ACNGT	8 cut(s)	341, 455, 760, 927, 1315, 1934, 2607, 2720
Bst6I	CTCTTC	2 cut(s)	501, 1938
BstACI	GRCGYC	1 cut(s)	400
BstAPI	GCANNNNNTGC	2 cut(s)	74, 1094
BstAUI	TGTACA	4 cut(s)	2176, 2366, 2527, 2711
BstC8I	GCNNGC	4 cut(s)	66, 409, 1636, 2540
BstDEI	CTNAG	8 cut(s)	9, 122, 297, 1014, 2049, 2099, 2430, 2534
BstDSI	CCRYGG	1 cut(s)	2296
BstFNI	CGCG	1 cut(s)	2340
BstMAI	GTCTC	5 cut(s)	120, 483, 816, 885, 2458
BstMWI	GCNNNNNNNGC	8 cut(s)	74, 252, 329, 1094, 1148, 1505, 1839, 2539
BstNI	CCWGG	7 cut(s)	483, 675, 826, 1284, 1362, 1404, 1764
BstNSI	RCATGY	3 cut(s)	901, 1982, 2465
BstSCI	CCNGG	8 cut(s)	481, 673, 824, 1282, 1360, 1402, 1597, 1762
BstSFI	CTRYAG	4 cut(s)	636, 1933, 2105, 2592
BstSLI	GKGCMC	3 cut(s)	184, 1654, 1770
BstUI	CGCG	1 cut(s)	2340
BstV1I	GCAGC	9 cut(s)	156, 267, 593, 761, 1100, 1163, 1411, 2578, 2824
BstV2I	GAAGAC	1 cut(s)	1778
BstX2I	RGATCY	4 cut(s)	316, 2062, 2403, 2840
BstXI	CCANNNNNNTGG	2 cut(s)	1558, 2686
BstYI	RGATCY	4 cut(s)	316, 2062, 2403, 2840
BsuI	GTATCC	1 cut(s)	1689
BsuRI	GGCC	9 cut(s)	212, 727, 931, 1491, 1597, 1762, 1839, 2085, 2628
BtgI	CCRYGG	1 cut(s)	2296
BtsI	GCAGTG	1 cut(s)	1036
BtsIMutI	CAGTG	5 cut(s)	460, 765, 932, 1036, 1863
Cac8I	GCNNGC	4 cut(s)	66, 409, 1636, 2540
CaiI	CAGNNNCTG	2 cut(s)	35, 1289
CciI	TCATGA	2 cut(s)	1789, 2485
Cfr10I	RCCGGY	2 cut(s)	64, 1102
Cfr13I	GGNCC	7 cut(s)	440, 726, 1595, 1622, 2434, 2627, 2668
Csp6I	GTAC	7 cut(s)	671, 923, 1106, 2177, 2367, 2528, 2712
CspCI	CAANNNNNGTGG	4 cut(s)	154, 189, 908, 943
CviQI	GTAC	7 cut(s)	671, 923, 1106, 2177, 2367, 2528, 2712
DdeI	CTNAG	8 cut(s)	9, 122, 297, 1014, 2049, 2099, 2430, 2534
DraI	TTTAAA	1 cut(s)	1239
DriI	GACNNNNNGTC	1 cut(s)	12
EaeI	YGGCCR	4 cut(s)	210, 929, 1489, 1760
Eam1104I	CTCTTC	2 cut(s)	501, 1938
Eam1105I	GACNNNNNGTC	1 cut(s)	12
EarI	CTCTTC	2 cut(s)	501, 1938
EciI	GGCGGA	1 cut(s)	1242
Ecl136II	GAGCTC	1 cut(s)	1034
Eco130I	CCWWGG	2 cut(s)	722, 2296
Eco24I	GRGCYC	3 cut(s)	203, 1036, 2755
Eco31I	GGTCTC	1 cut(s)	120
Eco32I	GATATC	2 cut(s)	1274, 1861
Eco47I	GGWCC	4 cut(s)	440, 1622, 2434, 2668
Eco53kI	GAGCTC	1 cut(s)	1034
EcoICRI	GAGCTC	1 cut(s)	1034
EcoO109I	RGGNCCY	2 cut(s)	440, 1622
EcoRII	CCWGG	7 cut(s)	481, 673, 824, 1282, 1360, 1402, 1762
EcoRV	GATATC	2 cut(s)	1274, 1861
EcoT14I	CCWWGG	2 cut(s)	722, 2296
EcoT22I	ATGCAT	2 cut(s)	2325, 2767
EcoT38I	GRGCYC	3 cut(s)	203, 1036, 2755
ErhI	CCWWGG	2 cut(s)	722, 2296
Esp3I	CGTCTC	1 cut(s)	816
FalI	AAGNNNNNCTT	2 cut(s)	2157, 2189
FaqI	GGGAC	1 cut(s)	453
FauI	CCCGC	1 cut(s)	1339
FauNDI	CATATG	3 cut(s)	474, 1393, 1477
FbaI	TGATCA	2 cut(s)	520, 1891
FblI	GTMKAC	2 cut(s)	626, 735
FriOI	GRGCYC	3 cut(s)	203, 1036, 2755
FspBI	CTAG	5 cut(s)	264, 273, 723, 1512, 1554
GsaI	CCCAGC	3 cut(s)	235, 939, 2461
GsuI	CTGGAG	5 cut(s)	465, 591, 1094, 1266, 2378
HaeIII	GGCC	9 cut(s)	212, 727, 931, 1491, 1597, 1762, 1839, 2085, 2628
HapII	CCGG	4 cut(s)	65, 870, 1103, 1598
Hin1I	GRCGYC	1 cut(s)	400
HincII	GTYRAC	1 cut(s)	2723
HindII	GTYRAC	1 cut(s)	2723
HindIII	AAGCTT	1 cut(s)	1324
HpaII	CCGG	4 cut(s)	65, 870, 1103, 1598
HphI	GGTGA	5 cut(s)	624, 1058, 1350, 1680, 2597
Hpy166II	GTNNAC	6 cut(s)	182, 627, 736, 1125, 1652, 2723
Hpy8I	GTNNAC	6 cut(s)	182, 627, 736, 1125, 1652, 2723
Hpy99I	CGWCG	2 cut(s)	402, 811
HpyAV	CCTTC	4 cut(s)	1942, 2053, 2659, 2689
HpyCH4III	ACNGT	8 cut(s)	341, 455, 760, 927, 1315, 1934, 2607, 2720
HpyCH4IV	ACGT	2 cut(s)	400, 745
HpyF10VI	GCNNNNNNNGC	8 cut(s)	74, 252, 329, 1094, 1148, 1505, 1839, 2539
HpyF3I	CTNAG	8 cut(s)	9, 122, 297, 1014, 2049, 2099, 2430, 2534
HpySE526I	ACGT	2 cut(s)	400, 745
Hsp92I	GRCGYC	1 cut(s)	400
KflI	GGGWCCC	1 cut(s)	440
Kpn2I	TCCGGA	1 cut(s)	869
KpnI	GGTACC	1 cut(s)	674
KroI	GCCGGC	1 cut(s)	64
KroNI	GCCGGC	1 cut(s)	66
Ksp22I	TGATCA	2 cut(s)	520, 1891
LmnI	GCTCC	5 cut(s)	301, 828, 1031, 1504, 2359
Lsp1109I	GCAGC	9 cut(s)	156, 267, 593, 761, 1100, 1163, 1411, 2578, 2824
MaeI	CTAG	5 cut(s)	264, 273, 723, 1512, 1554
MaeII	ACGT	2 cut(s)	400, 745
MaeIII	GTNAC	2 cut(s)	2009, 2213
MboII	GAAGA	8 cut(s)	518, 623, 752, 1746, 1778, 1955, 2782, 2837
MfeI	CAATTG	1 cut(s)	587
MflI	RGATCY	4 cut(s)	316, 2062, 2403, 2840
MhlI	GDGCHC	8 cut(s)	184, 203, 833, 1036, 1654, 1770, 2425, 2755
MlsI	TGGCCA	3 cut(s)	212, 1491, 1762
MluNI	TGGCCA	3 cut(s)	212, 1491, 1762
MlyI	GAGTC	8 cut(s)	22, 233, 302, 518, 1481, 1792, 1912, 2405
MmeI	TCCRAC	3 cut(s)	1600, 1905, 2166
Mox20I	TGGCCA	3 cut(s)	212, 1491, 1762
Mph1103I	ATGCAT	2 cut(s)	2325, 2767
MroI	TCCGGA	1 cut(s)	869
MroNI	GCCGGC	1 cut(s)	64
MroXI	GAANNNNTTC	2 cut(s)	950, 1354
MscI	TGGCCA	3 cut(s)	212, 1491, 1762
MslI	CAYNNNNRTG	2 cut(s)	2328, 2760
Msp20I	TGGCCA	3 cut(s)	212, 1491, 1762
MspA1I	CMGCKG	3 cut(s)	752, 1091, 2533
MspI	CCGG	4 cut(s)	65, 870, 1103, 1598
MspR9I	CCNGG	8 cut(s)	483, 675, 826, 1284, 1362, 1404, 1599, 1764
MunI	CAATTG	1 cut(s)	587
MvaI	CCWGG	7 cut(s)	483, 675, 826, 1284, 1362, 1404, 1764
MvnI	CGCG	1 cut(s)	2340
MwoI	GCNNNNNNNGC	8 cut(s)	74, 252, 329, 1094, 1148, 1505, 1839, 2539
NaeI	GCCGGC	1 cut(s)	66
NciI	CCSGG	1 cut(s)	1599
NcoI	CCATGG	1 cut(s)	2296
NdeI	CATATG	3 cut(s)	474, 1393, 1477
NgoMIV	GCCGGC	1 cut(s)	64
NlaIV	GGNNCC	7 cut(s)	52, 441, 442, 672, 1624, 1769, 2842
NmuCI	GTSAC	1 cut(s)	2213
NsiI	ATGCAT	2 cut(s)	2325, 2767
NspI	RCATGY	3 cut(s)	901, 1982, 2465
PagI	TCATGA	2 cut(s)	1789, 2485
PdiI	GCCGGC	1 cut(s)	66
PdmI	GAANNNNTTC	2 cut(s)	950, 1354
PfeI	GAWTC	6 cut(s)	358, 394, 614, 866, 916, 2020
PflMI	CCANNNNNTGG	1 cut(s)	1661
PfoI	TCCNGGA	1 cut(s)	481
PleI	GAGTC	8 cut(s)	21, 233, 301, 517, 1480, 1791, 1911, 2405
PpsI	GAGTC	8 cut(s)	21, 233, 301, 517, 1480, 1791, 1911, 2405
PpuMI	RGGWCCY	2 cut(s)	440, 1622
PshBI	ATTAAT	3 cut(s)	216, 1991, 2739
PsiI	TTATAA	2 cut(s)	1806, 2282
Psp124BI	GAGCTC	1 cut(s)	1036
Psp5II	RGGWCCY	2 cut(s)	440, 1622
Psp6I	CCWGG	7 cut(s)	481, 673, 824, 1282, 1360, 1402, 1762
PspFI	CCCAGC	3 cut(s)	231, 935, 2457
PspGI	CCWGG	7 cut(s)	481, 673, 824, 1282, 1360, 1402, 1762
PspN4I	GGNNCC	7 cut(s)	52, 441, 442, 672, 1624, 1769, 2842
PspPI	GGNCC	7 cut(s)	440, 726, 1595, 1622, 2434, 2627, 2668
PspPPI	RGGWCCY	2 cut(s)	440, 1622
PstI	CTGCAG	2 cut(s)	2109, 2596
PstNI	CAGNNNCTG	2 cut(s)	35, 1289
PsuI	RGATCY	4 cut(s)	316, 2062, 2403, 2840
PvuII	CAGCTG	2 cut(s)	752, 2533
RsaI	GTAC	7 cut(s)	672, 924, 1107, 2178, 2368, 2529, 2713
RsaNI	GTAC	7 cut(s)	671, 923, 1106, 2177, 2367, 2528, 2712
RseI	CAYNNNNRTG	2 cut(s)	2328, 2760
SacI	GAGCTC	1 cut(s)	1036
Sau96I	GGNCC	7 cut(s)	440, 726, 1595, 1622, 2434, 2627, 2668
SchI	GAGTC	8 cut(s)	22, 233, 302, 518, 1481, 1792, 1912, 2405
ScrFI	CCNGG	8 cut(s)	483, 675, 826, 1284, 1362, 1404, 1599, 1764
SduI	GDGCHC	8 cut(s)	184, 203, 833, 1036, 1654, 1770, 2425, 2755
SfcI	CTRYAG	4 cut(s)	636, 1933, 2105, 2592
SinI	GGWCC	4 cut(s)	440, 1622, 2434, 2668
SmiMI	CAYNNNNRTG	2 cut(s)	2328, 2760
SmlI	CTYRAG	3 cut(s)	658, 1026, 1814
SmoI	CTYRAG	3 cut(s)	658, 1026, 1814
SpeI	ACTAGT	2 cut(s)	272, 1553
SsiI	CCGC	6 cut(s)	932, 1091, 1227, 1346, 1840, 2338
SspMI	CTAG	5 cut(s)	264, 273, 723, 1512, 1554
SstI	GAGCTC	1 cut(s)	1036
StyD4I	CCNGG	8 cut(s)	481, 673, 824, 1282, 1360, 1402, 1597, 1762
StyI	CCWWGG	2 cut(s)	722, 2296
TaaI	ACNGT	8 cut(s)	341, 455, 760, 927, 1315, 1934, 2607, 2720
TaiI	ACGT	2 cut(s)	403, 748
TaqI	TCGA	5 cut(s)	169, 397, 1336, 1906, 2803
TatI	WGTACW	5 cut(s)	922, 2176, 2366, 2527, 2711
TauI	GCSGC	3 cut(s)	934, 1842, 2340
TfiI	GAWTC	6 cut(s)	358, 394, 614, 866, 916, 2020
TscAI	CASTG	5 cut(s)	460, 765, 932, 1043, 1870
TseFI	GTSAC	1 cut(s)	2213
TseI	GCWGC	9 cut(s)	144, 255, 581, 749, 1088, 1151, 1399, 2591, 2812
Tsp45I	GTSAC	1 cut(s)	2213
TspDTI	ATGAA	8 cut(s)	128, 456, 624, 792, 890, 1778, 2502, 2678
TspGWI	ACGGA	1 cut(s)	1248
TspRI	CASTG	5 cut(s)	460, 765, 932, 1043, 1870
Van91I	CCANNNNNTGG	1 cut(s)	1661
VneI	GTGCAC	2 cut(s)	180, 1650
VpaK11BI	GGWCC	4 cut(s)	440, 1622, 2434, 2668
VspI	ATTAAT	3 cut(s)	216, 1991, 2739
XapI	RAATTY	6 cut(s)	1082, 1603, 1800, 1880, 2088, 2546
XbaI	TCTAGA	1 cut(s)	1511
XceI	RCATGY	3 cut(s)	901, 1982, 2465
XcmI	CCANNNNNNNNNTGG	1 cut(s)	239
XmaJI	CCTAGG	1 cut(s)	722
XmiI	GTMKAC	2 cut(s)	626, 735
XmnI	GAANNNNTTC	2 cut(s)	950, 1354
XspI	CTAG	5 cut(s)	264, 273, 723, 1512, 1554
ZraI	GACGTC	1 cut(s)	401
Zsp2I	ATGCAT	2 cut(s)	2325, 2767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.