Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0018719

Physical Location & Seq

Genomic Coordinates Forward (+)

13542918 .. 13548629

Gene Structure 5712 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G26970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2988 bp

ATGTATCGACGCGCCACTTCCGGCGTGCGTTCTGCCTCCGCAAGGCTTTCTTCTTCCCTCTCCAGGATCGCCTCTAGCGAGACAGCTTCGGTTTCGGCTCCATCTGCGTCGTCGCTTCGCAATCAGACGAACCGATCTAAGAGTTTCTCCTCGGCTCTCCGCTCCTTTCGAGTTTGCTCTGCTTCCACGCGGTGGAGCCATGGTGGGAGCTGGGGATCGCCTGCTAGCCTTCGCGCTCAAGCTAGGAATTCCACTCCGGTTATGGAGAAATTTGAACGGAAATACGCAACCATGGCTTCTGAGCATTCCTACAAGGATATTTTGACAAGTCTTCCTAAACCTGGTGGTGGAGAGTATGGAAAGTACTACTCGTTACCAGCTTTAAACGATCCAAGAATTGATAAGCTGCCATTTTCTGTAAGGATACTATTGGAATCAGCGATTCGTAACTGTGACAACTATCAAGTCACAAAGGATGACGTTGAGAAAATCCTTGACTGGGAGAATACATCTACTAAGCAGGTTGAAATTGCCTTCAAGCCAGCCCGTGTTATCTTACAGGACTTCACTGGAGTACCAGTTCTAGTTGATCTTGCTTCTATGAGGGATGCAGTGAAGAATCTTGGTAGTGATCCTAGCAAGATCAACCCCTTGGTTCCTGTTGATCTTGTCGTTGATCACTCGATCCAAGTTGACTTTGCAAGGTCAGAAGATGCCGCACAGAAAAATTTGGAGCTTGAATTCAAAAGGAACAAAGAAAGATTTACGTTCCTTAAGTGGGGTTCAACAGCCTTCCAAAACATGCTGGTCGTTCCTCCTGGGTCTGGGATTGTCCATCAGGTCAACTTGGAATACCTTGGACGTGTTGTTTTCAACTCGAAAGGATTTCTCTACCCTGATAGCGTTGTTGGAACCGACTCCCACACAACCATGATTGATGGATTAGGAGTCGCTGGGTGGGGTGTTGGAGGAATTGAGGCAGAGGCAGCAATGCTTGGTCAGCCCATGAGCATGGTGTTACCTGGCGTGGTCGGGTTTAAGTTGGATGGAAAGTTGAAGGAAGGGGTTACTGCTACTGATTTAGTTCTCACTGTGACCCAAATATTGAGGAAGCATGGTGTTGTCGGCAAGTTTGTTGAATTTTATGGTGAGGGGATGAGTGAACTTTCACTGGCTGATAGAGCCACAATTGCAAATATGTCTCCCGAGTATGGAGCAACTATGGGTTTCTTCCCAGTGGATCATGTTACACTGGAGTACCTGAAGTTGACAGGAAGAAGCGATGAAACTGTGTCAATGATAGAATCATATTTGCGCGCAAACAATATGTTTGTTGACTACAACGAGCCTCAGCAAGAAAGAGCATACACATCTTATCTGCAGTTAGATTTGGGACATGTTGAACCATGTATTTCTGGTCCTAAAAGGCCTCATGACCGAGTGCCTCTGAAAGATATGAAGGCTGACTGGCATGCATGCCTTGACAATCCTGTTGGATTCAAGGGTTTTGCAGTGCCAAAAGAAAAACAAGAAGAAGTTGTGAAGTTCTCATACAACGGACAACCTGCTGAGATCAAGCATGGTAGTGTTGTTATTGCGGCAATTACTAGTTGTACAAACACGTCAAACCCTAGTGTCATGATCGGAGCAGCACTCGTTGCAAAAAAAGCTTCAGATCTTGGCCTGAAGGTAAAACCATGGGTTAAGACAAGTCTTGCTCCAGGGTCTAGAGTTGTCGAGAAATATTTGGATCGAAGTGGGCTCCGAGAGTCCTTGACCAAGCAAGGATTCGAAATTGTCGGCTACGGCTGCACAACATGCATTGGGAATTCTGGCAACCTTGACCCAGAAGTTGCATCTGCTATAGAAGGAACTGATATCATCCCAGCTGCTGTGCTGTCTGGAAACCGAAACTTTGAAGGTCGTGTTCATCCACAAACAAGAGCAAACTATCTTGCGTCACCACCATTAGTTGTTGCTTATGCCCTTGCTGGAACGGTGGACATTGATTTTGAGAAAGAGCCTATAGGAACCAGAAGTGATGGAAAAAGTGTGTACCTGAGGGATGTATGGCCAAGCAATGAGGAAGTTGCTCAGGTTGTTCAATATAGCGTGCTACCAAGCATGTTCAAGAGCTCGTACGAAACAATAACAGAGGGAAACCCCTTGTGGAATGAACTCTCTGCACCGAGTTCCACGTTATATTCTTGGGATCCAAACTCCACGTACATTCATGAACCTCCGTATTTCAAGAATATGACCGCAAATCCACCTGGTCCTCGTGAAGTGAAGGATGCTTACTGCTTGTTGAACTTTGGGGACAGTGTAACAACAGACCACATCTCTCCAGCAGGAAACATTCAAAAAACTAGTCCTGCTGCAAAGTTTCTAATGGACCGTGGTGTGATTTCGGAAGATTTCAACTCATATGGAAGTCGAAGGGGAAACGACGAGGTTATGGCTCGTGGTACATTTGCCAATATCCGTATTGTTAACAAGCTCTTGAAAGGAGAAGTTGGCCCCAACACTGTTCACATTCCGACTGGAGAGAAGCTTAGCGTTTTCGATGCAGCAAGTAAATACAAGACCGCTGAACAGGATACGATCATCCTGGCTGGTGCTGAGTATGGAAGTGGTAGCTCTCGGGATTGGGCTGCAAAGGGTCCCTTGCTTTTGGGAGTGAAAGCTGTAATTGCCAAGAGCTTTGAGAGAATCCATCGCAGCAACCTGGCTGGTATGGGGATCATTCCTCTGTGTTTCAAGGCTGGGGAGGACGCGGAAACCCTTGGACTCACTGGTCATGAACGCTACACAGTCCACCTTCCTACAAAAGTTAGTGACATTAGACCCGGTCAAGACGTCACTGTAACCACTGACAGTGGCAAATCCTTTGTCTGCACCCTGCGTTTTGATACAGAGGTGGAATTGGCATACTATGATCACGGCGGTATTCTACCATACGTCATCCGGAGTTTGAGCGCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0003674 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0006979 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030312 GO:0030554 GO:0031090 GO:0031667 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0033993 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048856 GO:0050896 GO:0071704 GO:0071944 GO:0072350 GO:0090351 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1901700 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

995

Amino Acids

108.48

Weight (kDa)

6.71

Isoelectric Point (pI)

38.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	162 - 665	1.1e-171	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	794 - 922	1.8e-39	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0018719)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26970
malus_domestica	MD03G1199800.v1.1
rosa_multiflora	Rmu_sc0000103.1_g000062
rosa_roxburghii	Rroxscaffold_1G00041170
rosa_samantha	Rh5AG270700 Rh5BG273700 Rh5BG273800 Rh5CG306800
rosa_wichuraiana	Rw5G025260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2796
AatII	GACGTC	1 cut(s)	2862
Acc36I	ACCTGC	2 cut(s)	511, 1573
AccB7I	CCANNNNNTGG	1 cut(s)	192
AccBSI	CCGCTC	1 cut(s)	162
AccII	CGCG	5 cut(s)	12, 190, 234, 1317, 2777
AccIII	TCCGGA	1 cut(s)	2967
AciI	CCGC	9 cut(s)	39, 160, 190, 717, 1598, 2262, 2589, 2777, 2946
AcoI	YGGCCR	1 cut(s)	2072
AcsI	RAATTY	6 cut(s)	247, 269, 727, 740, 1139, 1828
AcuI	CTGAAG	3 cut(s)	1283, 1656, 1706
AcyI	GRCGYC	1 cut(s)	2859
AdeI	CACNNNGTG	1 cut(s)	192
AfaI	GTAC	8 cut(s)	365, 576, 1259, 1615, 2057, 2141, 2228, 2470
AfiI	CCNNNNNNNGG	9 cut(s)	42, 63, 192, 341, 347, 499, 778, 824, 1211
AflII	CTTAAG	1 cut(s)	773
AflIII	ACRYGT	3 cut(s)	862, 1396, 1620
AhlI	ACTAGT	2 cut(s)	1607, 2369
AjiI	CACGTC	2 cut(s)	863, 1623
AjnI	CCWGG	8 cut(s)	62, 340, 817, 1021, 1720, 2272, 2610, 2727
AjuI	GAANNNNNNNTTGG	2 cut(s)	1772, 1804
AloI	GAACNNNNNNTCC	2 cut(s)	564, 596
Alw21I	GWGCWC	1 cut(s)	2138
Alw26I	GTCTC	2 cut(s)	74, 1206
AlwNI	CAGNNNCTG	1 cut(s)	1892
Ama87I	CYCGRG	2 cut(s)	1205, 2643
Aor13HI	TCCGGA	1 cut(s)	2967
AoxI	GGCC	4 cut(s)	1427, 1681, 2072, 2518
ApeKI	GCWGC	9 cut(s)	406, 986, 1649, 1809, 1889, 2378, 2570, 2654, 2721
ApoI	RAATTY	6 cut(s)	247, 269, 727, 740, 1139, 1828
ArsI	GACNNNNNNTTYG	6 cut(s)	1994, 2026, 2356, 2388, 2877, 2909
AspLEI	GCGC	5 cut(s)	14, 236, 1317, 1319, 2981
AspS9I	GGNCC	5 cut(s)	1418, 2276, 2395, 2519, 2663
AsuC2I	CCSGG	1 cut(s)	2850
AsuHPI	GGTGA	2 cut(s)	1160, 1953
AsuII	TTCGAA	1 cut(s)	1791
AsuNHI	GCTAGC	1 cut(s)	224
AvaI	CYCGRG	2 cut(s)	1205, 2643
AvaII	GGWCC	4 cut(s)	1418, 2276, 2395, 2663
AxyI	CCTNAGG	1 cut(s)	2060
BaeI	ACNNNNGTAYC	6 cut(s)	558, 591, 1241, 1274, 2904, 2937
BalI	TGGCCA	1 cut(s)	2074
BamHI	GGATCC	1 cut(s)	2212
BanII	GRGCYC	2 cut(s)	1764, 2138
BarI	GAAGNNNNNNTAC	4 cut(s)	1535, 1567, 2282, 2314
BauI	CACGAG	2 cut(s)	2280, 2463
BbsI	GAAGAC	1 cut(s)	323
Bbv12I	GWGCWC	1 cut(s)	2138
BbvCI	CCTCAGC	1 cut(s)	1350
BbvI	GCAGC	9 cut(s)	393, 998, 1661, 1796, 1876, 2365, 2582, 2641, 2733
BccI	CCATC	6 cut(s)	109, 843, 932, 1040, 2036, 2724
BceAI	ACGGC	2 cut(s)	1822, 2959
BciT130I	CCWGG	8 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729
BciVI	GTATCC	2 cut(s)	417, 2593
BclI	TGATCA	2 cut(s)	676, 2938
BcnI	CCSGG	1 cut(s)	2850
BcoDI	GTCTC	2 cut(s)	74, 1206
BcuI	ACTAGT	2 cut(s)	1607, 2369
BfaI	CTAG	9 cut(s)	75, 225, 243, 584, 636, 1608, 1632, 1728, 2370
BfmI	CTRYAG	3 cut(s)	1379, 1863, 2025
BfoI	RGCGCY	1 cut(s)	2982
BfrI	CTTAAG	1 cut(s)	773
BfuAI	ACCTGC	2 cut(s)	511, 1573
BfuI	GTATCC	2 cut(s)	417, 2593
BglII	AGATCT	1 cut(s)	1675
BlpI	GCTNAGC	1 cut(s)	2555
BmcAI	AGTACT	1 cut(s)	365
Bme1390I	CCNGG	9 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729, 2850
Bme18I	GGWCC	4 cut(s)	1418, 2276, 2395, 2663
BmeT110I	CYCGRG	2 cut(s)	1205, 2643
BmgBI	CACGTC	2 cut(s)	863, 1623
BmgT120I	GGNCC	5 cut(s)	1418, 2276, 2395, 2519, 2663
BmrFI	CCNGG	9 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729, 2850
BmrI	ACTGGG	2 cut(s)	508, 1229
BmsI	GCATC	5 cut(s)	598, 703, 1865, 2284, 2557
BmtI	GCTAGC	1 cut(s)	228
BmuI	ACTGGG	2 cut(s)	508, 1229
BpiI	GAAGAC	1 cut(s)	323
BplI	GAGNNNNNCTC	2 cut(s)	1638, 1670
BpmI	CTGGAG	6 cut(s)	46, 591, 1274, 1704, 2331, 2565
Bpu10I	CCTNAGC	2 cut(s)	1350, 2094
Bpu1102I	GCTNAGC	1 cut(s)	2555
Bpu14I	TTCGAA	1 cut(s)	1791
BpuEI	CTTGAG	1 cut(s)	222
BpuMI	CCSGG	1 cut(s)	2850
BsaAI	YACGTR	1 cut(s)	2226
BsaHI	GRCGYC	1 cut(s)	2859
BsaWI	WCCGGW	2 cut(s)	256, 2967
BsaXI	ACNNNNNCTCC	4 cut(s)	187, 217, 564, 594
Bsc4I	CCNNNNNNNGG	9 cut(s)	42, 63, 192, 341, 347, 499, 778, 824, 1211
Bse1I	ACTGG	9 cut(s)	503, 574, 578, 1176, 1235, 1257, 1472, 2548, 2800
Bse21I	CCTNAGG	1 cut(s)	2060
Bse3DI	GCAATG	2 cut(s)	996, 2086
BseAI	TCCGGA	1 cut(s)	2967
BseBI	CCWGG	8 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729
BseLI	CCNNNNNNNGG	9 cut(s)	42, 63, 192, 341, 347, 499, 778, 824, 1211
BseMI	GCAATG	2 cut(s)	996, 2086
BseMII	CTCAG	6 cut(s)	291, 1364, 1560, 2051, 2108, 2613
BseNI	ACTGG	9 cut(s)	503, 574, 578, 1176, 1235, 1257, 1472, 2548, 2800
BsePI	GCGCGC	1 cut(s)	1315
BseRI	GAGGAG	1 cut(s)	139
BseXI	GCAGC	9 cut(s)	393, 998, 1661, 1796, 1876, 2365, 2582, 2641, 2733
BseYI	CCCAGC	4 cut(s)	210, 953, 1885, 2765
BsgI	GTGCAG	3 cut(s)	1795, 2169, 2881
Bsh1236I	CGCG	5 cut(s)	12, 190, 234, 1317, 2777
BshFI	GGCC	4 cut(s)	1429, 1683, 2074, 2520
BsiHKAI	GWGCWC	1 cut(s)	2138
BsiHKCI	CYCGRG	2 cut(s)	1205, 2643
BsiSI	CCGG	4 cut(s)	21, 257, 2850, 2968
BsiWI	CGTACG	1 cut(s)	2139
BslFI	GGGAC	3 cut(s)	1407, 2333, 2649
BslI	CCNNNNNNNGG	9 cut(s)	42, 63, 192, 341, 347, 499, 778, 824, 1211
BsmAI	GTCTC	2 cut(s)	74, 1206
BsmFI	GGGAC	3 cut(s)	1407, 2333, 2649
BsmI	GAATGC	1 cut(s)	304
BsnI	GGCC	4 cut(s)	1429, 1683, 2074, 2520
BsoBI	CYCGRG	2 cut(s)	1205, 2643
Bsp119I	TTCGAA	1 cut(s)	1791
Bsp1286I	GDGCHC	2 cut(s)	1764, 2138
Bsp13I	TCCGGA	1 cut(s)	2967
Bsp1407I	TGTACA	1 cut(s)	1613
Bsp1720I	GCTNAGC	1 cut(s)	2555
Bsp19I	CCATGG	3 cut(s)	199, 291, 1697
BspACI	CCGC	9 cut(s)	39, 160, 190, 717, 1598, 2262, 2589, 2777, 2946
BspANI	GGCC	4 cut(s)	1429, 1683, 2074, 2520
BspCNI	CTCAG	6 cut(s)	292, 1363, 1561, 2052, 2107, 2614
BspEI	TCCGGA	1 cut(s)	2967
BspFNI	CGCG	5 cut(s)	12, 190, 234, 1317, 2777
BspHI	TCATGA	4 cut(s)	1432, 1638, 2233, 2800
BspMAI	CTGCAG	1 cut(s)	1383
BspMI	ACCTGC	2 cut(s)	511, 1573
BspOI	GCTAGC	1 cut(s)	228
BspT104I	TTCGAA	1 cut(s)	1791
BspTI	CTTAAG	1 cut(s)	773
BsrBI	CCGCTC	1 cut(s)	162
BsrDI	GCAATG	2 cut(s)	996, 2086
BsrGI	TGTACA	1 cut(s)	1613
BsrI	ACTGG	9 cut(s)	503, 574, 578, 1176, 1235, 1257, 1472, 2548, 2800
BssHII	GCGCGC	1 cut(s)	1315
BssNI	GRCGYC	1 cut(s)	2859
BssSI	CACGAG	2 cut(s)	2280, 2463
BssT1I	CCWWGG	6 cut(s)	199, 291, 651, 856, 1697, 2785
Bst2BI	CACGAG	2 cut(s)	2280, 2463
Bst2UI	CCWGG	8 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729
BstACI	GRCGYC	1 cut(s)	2859
BstAFI	CTTAAG	1 cut(s)	773
BstAPI	GCANNNNNTGC	2 cut(s)	1658, 1818
BstAUI	TGTACA	1 cut(s)	1613
BstBAI	YACGTR	1 cut(s)	2226
BstBI	TTCGAA	1 cut(s)	1791
BstC8I	GCNNGC	8 cut(s)	26, 222, 226, 543, 1317, 1473, 1477, 2114
BstDEI	CTNAG	9 cut(s)	138, 300, 516, 1350, 1569, 2060, 2094, 2555, 2622
BstDSI	CCRYGG	4 cut(s)	199, 291, 1697, 2398
BstENI	CCTNNNNNAGG	1 cut(s)	40
BstFNI	CGCG	5 cut(s)	12, 190, 234, 1317, 2777
BstH2I	RGCGCY	1 cut(s)	2982
BstHHI	GCGC	5 cut(s)	14, 236, 1317, 1319, 2981
BstMAI	GTCTC	2 cut(s)	74, 1206
BstNI	CCWGG	8 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729
BstNSI	RCATGY	6 cut(s)	805, 1400, 1475, 1479, 1821, 2128
BstSCI	CCNGG	9 cut(s)	62, 340, 817, 1021, 1720, 2272, 2610, 2727, 2848
BstSFI	CTRYAG	3 cut(s)	1379, 1863, 2025
BstUI	CGCG	5 cut(s)	12, 190, 234, 1317, 2777
BstV1I	GCAGC	9 cut(s)	393, 998, 1661, 1796, 1876, 2365, 2582, 2641, 2733
BstV2I	GAAGAC	1 cut(s)	323
BstX2I	RGATCY	2 cut(s)	1675, 2212
BstXI	CCANNNNNNTGG	1 cut(s)	1012
BstYI	RGATCY	2 cut(s)	1675, 2212
Bsu36I	CCTNAGG	1 cut(s)	2060
BsuI	GTATCC	2 cut(s)	417, 2593
BsuRI	GGCC	4 cut(s)	1429, 1683, 2074, 2520
BtgI	CCRYGG	4 cut(s)	199, 291, 1697, 2398
BtgZI	GCGATG	2 cut(s)	1296, 2702
BtrI	CACGTC	2 cut(s)	863, 1623
BtsI	GCAGTG	2 cut(s)	618, 1518
BveI	ACCTGC	2 cut(s)	511, 1573
Cac8I	GCNNGC	8 cut(s)	26, 222, 226, 543, 1317, 1473, 1477, 2114
CaiI	CAGNNNCTG	1 cut(s)	1892
CciI	TCATGA	4 cut(s)	1432, 1638, 2233, 2800
CfoI	GCGC	5 cut(s)	14, 236, 1317, 1319, 2981
Cfr13I	GGNCC	5 cut(s)	1418, 2276, 2395, 2519, 2663
CseI	GACGC	4 cut(s)	18, 96, 1947, 2783
CsiI	ACCWGGT	2 cut(s)	340, 2272
Csp6I	GTAC	8 cut(s)	364, 575, 1258, 1614, 2056, 2140, 2227, 2469
CviQI	GTAC	8 cut(s)	364, 575, 1258, 1614, 2056, 2140, 2227, 2469
DdeI	CTNAG	9 cut(s)	138, 300, 516, 1350, 1569, 2060, 2094, 2555, 2622
DraI	TTTAAA	1 cut(s)	384
DraIII	CACNNNGTG	1 cut(s)	192
DrdI	GACNNNNNNGTC	1 cut(s)	2796
DseDI	GACNNNNNNGTC	1 cut(s)	2796
EaeI	YGGCCR	1 cut(s)	2072
Ecl136II	GAGCTC	1 cut(s)	2136
Eco130I	CCWWGG	6 cut(s)	199, 291, 651, 856, 1697, 2785
Eco147I	AGGCCT	1 cut(s)	1429
Eco24I	GRGCYC	2 cut(s)	1764, 2138
Eco32I	GATATC	1 cut(s)	1879
Eco47I	GGWCC	4 cut(s)	1418, 2276, 2395, 2663
Eco53kI	GAGCTC	1 cut(s)	2136
Eco57I	CTGAAG	3 cut(s)	1283, 1656, 1706
Eco81I	CCTNAGG	1 cut(s)	2060
Eco88I	CYCGRG	2 cut(s)	1205, 2643
EcoICRI	GAGCTC	1 cut(s)	2136
EcoNI	CCTNNNNNAGG	1 cut(s)	40
EcoO109I	RGGNCCY	1 cut(s)	2663
EcoRI	GAATTC	3 cut(s)	247, 740, 1828
EcoRII	CCWGG	8 cut(s)	62, 340, 817, 1021, 1720, 2272, 2610, 2727
EcoRV	GATATC	1 cut(s)	1879
EcoT14I	CCWWGG	6 cut(s)	199, 291, 651, 856, 1697, 2785
EcoT22I	ATGCAT	2 cut(s)	1477, 1823
EcoT38I	GRGCYC	2 cut(s)	1764, 2138
ErhI	CCWWGG	6 cut(s)	199, 291, 651, 856, 1697, 2785
FalI	AAGNNNNNCTT	4 cut(s)	34, 66, 2651, 2683
FaqI	GGGAC	3 cut(s)	1407, 2333, 2649
FauNDI	CATATG	1 cut(s)	2428
FbaI	TGATCA	2 cut(s)	676, 2938
FriOI	GRGCYC	2 cut(s)	1764, 2138
FspBI	CTAG	9 cut(s)	75, 225, 243, 584, 636, 1608, 1632, 1728, 2370
GlaI	GCGC	5 cut(s)	13, 235, 1316, 1318, 2980
GsaI	CCCAGC	4 cut(s)	214, 957, 1889, 2769
GsuI	CTGGAG	6 cut(s)	46, 591, 1274, 1704, 2331, 2565
HaeII	RGCGCY	1 cut(s)	2982
HaeIII	GGCC	4 cut(s)	1429, 1683, 2074, 2520
HapII	CCGG	4 cut(s)	21, 257, 2850, 2968
HgaI	GACGC	4 cut(s)	18, 96, 1947, 2783
HhaI	GCGC	5 cut(s)	14, 236, 1317, 1319, 2981
Hin1I	GRCGYC	1 cut(s)	2859
Hin6I	GCGC	5 cut(s)	12, 234, 1315, 1317, 2979
HinP1I	GCGC	5 cut(s)	12, 234, 1315, 1317, 2979
HincII	GTYRAC	5 cut(s)	694, 844, 1269, 1336, 2494
HindII	GTYRAC	5 cut(s)	694, 844, 1269, 1336, 2494
HindIII	AAGCTT	2 cut(s)	1668, 2552
HpaI	GTTAAC	1 cut(s)	2494
HpaII	CCGG	4 cut(s)	21, 257, 2850, 2968
HphI	GGTGA	2 cut(s)	1160, 1953
Hpy188I	TCNGA	9 cut(s)	126, 301, 709, 1449, 1646, 1675, 1766, 2413, 2541
Hpy99I	CGWCG	4 cut(s)	12, 112, 115, 2453
HpyCH4IV	ACGT	8 cut(s)	480, 767, 862, 1622, 2198, 2225, 2859, 2961
HpyF3I	CTNAG	9 cut(s)	138, 300, 516, 1350, 1569, 2060, 2094, 2555, 2622
HpySE526I	ACGT	8 cut(s)	480, 767, 862, 1622, 2198, 2225, 2859, 2961
Hsp92I	GRCGYC	1 cut(s)	2859
HspAI	GCGC	5 cut(s)	12, 234, 1315, 1317, 2979
KflI	GGGWCCC	1 cut(s)	2663
Kpn2I	TCCGGA	1 cut(s)	2967
Ksp22I	TGATCA	2 cut(s)	676, 2938
KspAI	GTTAAC	1 cut(s)	2494
LmnI	GCTCC	9 cut(s)	103, 167, 195, 207, 733, 1214, 1646, 1723, 1767
Lsp1109I	GCAGC	9 cut(s)	393, 998, 1661, 1796, 1876, 2365, 2582, 2641, 2733
LweI	GCATC	5 cut(s)	598, 703, 1865, 2284, 2557
MabI	ACCWGGT	2 cut(s)	340, 2272
MaeI	CTAG	9 cut(s)	75, 225, 243, 584, 636, 1608, 1632, 1728, 2370
MaeII	ACGT	8 cut(s)	480, 767, 862, 1622, 2198, 2225, 2859, 2961
MbiI	CCGCTC	1 cut(s)	162
MboII	GAAGA	9 cut(s)	42, 45, 323, 628, 722, 1222, 1287, 1544, 2426
MfeI	CAATTG	1 cut(s)	1188
MflI	RGATCY	2 cut(s)	1675, 2212
MhlI	GDGCHC	2 cut(s)	1764, 2138
MlsI	TGGCCA	1 cut(s)	2074
MluNI	TGGCCA	1 cut(s)	2074
MlyI	GAGTC	4 cut(s)	911, 957, 1778, 2784
MmeI	TCCRAC	5 cut(s)	889, 946, 1023, 1474, 2564
Mox20I	TGGCCA	1 cut(s)	2074
Mph1103I	ATGCAT	2 cut(s)	1477, 1823
MroI	TCCGGA	1 cut(s)	2967
MscI	TGGCCA	1 cut(s)	2074
MseI	TTAA	5 cut(s)	383, 774, 1038, 1704, 2493
MslI	CAYNNNNRTG	3 cut(s)	929, 1010, 1584
Msp20I	TGGCCA	1 cut(s)	2074
MspA1I	CMGCKG	2 cut(s)	1889, 2591
MspCI	CTTAAG	1 cut(s)	773
MspI	CCGG	4 cut(s)	21, 257, 2850, 2968
MspR9I	CCNGG	9 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729, 2850
MunI	CAATTG	1 cut(s)	1188
Mva1269I	GAATGC	1 cut(s)	304
MvaI	CCWGG	8 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729
MvnI	CGCG	5 cut(s)	12, 190, 234, 1317, 2777
NciI	CCSGG	1 cut(s)	2850
NcoI	CCATGG	3 cut(s)	199, 291, 1697
NdeI	CATATG	1 cut(s)	2428
NheI	GCTAGC	1 cut(s)	224
NmeAIII	GCCGAG	1 cut(s)	131
NmuCI	GTSAC	6 cut(s)	452, 466, 1093, 1959, 2837, 2860
NsiI	ATGCAT	2 cut(s)	1477, 1823
NspI	RCATGY	6 cut(s)	805, 1400, 1475, 1479, 1821, 2128
NspV	TTCGAA	1 cut(s)	1791
PaeI	GCATGC	2 cut(s)	1475, 1479
PagI	TCATGA	4 cut(s)	1432, 1638, 2233, 2800
PauI	GCGCGC	1 cut(s)	1315
PceI	AGGCCT	1 cut(s)	1429
PciI	ACATGT	1 cut(s)	1396
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	304
PfeI	GAWTC	7 cut(s)	434, 442, 619, 1304, 1497, 1788, 2712
Pfl23II	CGTACG	1 cut(s)	2139
PflFI	GACNNNGTC	1 cut(s)	2850
PflMI	CCANNNNNTGG	1 cut(s)	192
PfoI	TCCNGGA	1 cut(s)	62
PleI	GAGTC	4 cut(s)	911, 956, 1777, 2784
PpsI	GAGTC	4 cut(s)	911, 956, 1777, 2784
Ppu21I	YACGTR	1 cut(s)	2226
PpuMI	RGGWCCY	1 cut(s)	2663
PscI	ACATGT	1 cut(s)	1396
Psp124BI	GAGCTC	1 cut(s)	2138
Psp5II	RGGWCCY	1 cut(s)	2663
Psp6I	CCWGG	8 cut(s)	62, 340, 817, 1021, 1720, 2272, 2610, 2727
PspFI	CCCAGC	4 cut(s)	210, 953, 1885, 2765
PspGI	CCWGG	8 cut(s)	62, 340, 817, 1021, 1720, 2272, 2610, 2727
PspLI	CGTACG	1 cut(s)	2139
PspPI	GGNCC	5 cut(s)	1418, 2276, 2395, 2519, 2663
PspPPI	RGGWCCY	1 cut(s)	2663
PstI	CTGCAG	1 cut(s)	1383
PstNI	CAGNNNCTG	1 cut(s)	1892
PsuI	RGATCY	2 cut(s)	1675, 2212
PsyI	GACNNNGTC	1 cut(s)	2850
PteI	GCGCGC	1 cut(s)	1315
PvuII	CAGCTG	1 cut(s)	1889
RsaI	GTAC	8 cut(s)	365, 576, 1259, 1615, 2057, 2141, 2228, 2470
RsaNI	GTAC	8 cut(s)	364, 575, 1258, 1614, 2056, 2140, 2227, 2469
RseI	CAYNNNNRTG	3 cut(s)	929, 1010, 1584
SacI	GAGCTC	1 cut(s)	2138
SaqAI	TTAA	5 cut(s)	383, 774, 1038, 1704, 2493
Sau96I	GGNCC	5 cut(s)	1418, 2276, 2395, 2519, 2663
ScaI	AGTACT	1 cut(s)	365
SchI	GAGTC	4 cut(s)	911, 957, 1778, 2784
ScrFI	CCNGG	9 cut(s)	64, 342, 819, 1023, 1722, 2274, 2612, 2729, 2850
SduI	GDGCHC	2 cut(s)	1764, 2138
SexAI	ACCWGGT	2 cut(s)	340, 2272
SfaNI	GCATC	5 cut(s)	598, 703, 1865, 2284, 2557
SfcI	CTRYAG	3 cut(s)	1379, 1863, 2025
SfuI	TTCGAA	1 cut(s)	1791
SinI	GGWCC	4 cut(s)	1418, 2276, 2395, 2663
SmiMI	CAYNNNNRTG	3 cut(s)	929, 1010, 1584
SmlI	CTYRAG	2 cut(s)	237, 773
SmoI	CTYRAG	2 cut(s)	237, 773
SpeI	ACTAGT	2 cut(s)	1607, 2369
SphI	GCATGC	2 cut(s)	1475, 1479
SseBI	AGGCCT	1 cut(s)	1429
SsiI	CCGC	9 cut(s)	39, 160, 190, 717, 1598, 2262, 2589, 2777, 2946
SspI	AATATT	2 cut(s)	1104, 1745
SspMI	CTAG	9 cut(s)	75, 225, 243, 584, 636, 1608, 1632, 1728, 2370
SstI	GAGCTC	1 cut(s)	2138
StuI	AGGCCT	1 cut(s)	1429
StyD4I	CCNGG	9 cut(s)	62, 340, 817, 1021, 1720, 2272, 2610, 2727, 2848
StyI	CCWWGG	6 cut(s)	199, 291, 651, 856, 1697, 2785
TaiI	ACGT	8 cut(s)	483, 770, 865, 1625, 2201, 2228, 2862, 2964
TaqI	TCGA	9 cut(s)	7, 169, 683, 878, 1737, 1753, 1791, 2437, 2565
TaqII	GACCGA	1 cut(s)	1452
TatI	WGTACW	2 cut(s)	363, 1613
TauI	GCSGC	2 cut(s)	719, 1601
TfiI	GAWTC	7 cut(s)	434, 442, 619, 1304, 1497, 1788, 2712
Tru1I	TTAA	5 cut(s)	383, 774, 1038, 1704, 2493
Tru9I	TTAA	5 cut(s)	383, 774, 1038, 1704, 2493
TseFI	GTSAC	6 cut(s)	452, 466, 1093, 1959, 2837, 2860
TseI	GCWGC	9 cut(s)	406, 986, 1649, 1809, 1889, 2378, 2570, 2654, 2721
Tsp45I	GTSAC	6 cut(s)	452, 466, 1093, 1959, 2837, 2860
TspDTI	ATGAA	7 cut(s)	1299, 1472, 1919, 2190, 2222, 2250, 2817
TspGWI	ACGGA	4 cut(s)	292, 1572, 2232, 2474
Tth111I	GACNNNGTC	1 cut(s)	2850
Van91I	CCANNNNNTGG	1 cut(s)	192
Vha464I	CTTAAG	1 cut(s)	773
VpaK11BI	GGWCC	4 cut(s)	1418, 2276, 2395, 2663
XagI	CCTNNNNNAGG	1 cut(s)	40
XapI	RAATTY	6 cut(s)	247, 269, 727, 740, 1139, 1828
XbaI	TCTAGA	1 cut(s)	1727
XceI	RCATGY	6 cut(s)	805, 1400, 1475, 1479, 1821, 2128
XcmI	CCANNNNNNNNNTGG	1 cut(s)	259
XspI	CTAG	9 cut(s)	75, 225, 243, 584, 636, 1608, 1632, 1728, 2370
ZraI	GACGTC	1 cut(s)	2860
ZrmI	AGTACT	1 cut(s)	365
Zsp2I	ATGCAT	2 cut(s)	1477, 1823

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.