Rw5G025260

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018719
Physical Location & Seq
Reverse (-)
35923807 .. 35929141
5335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025260.1

Sequence Viewer

Length: 1317 bp
ATGGCTTCTCAACATGCTATTGAGGGAATATTAAACAGTCTCCCAAAGCCTGGAGGTGGCAAATTCGGAAAATTTTACAACAGGCTTCCATATTCAATAAAGATACTTTTGGAATCTGCAATCCATAACTGTGACAACTTCCAAATTACCAAGGAAGATGTTGAAAAGATGGCTGATTGGGAGAATACATCACCAAAGCAAGTTGAAATACCCTTTAAGCCTGCTCGTGTTCTCCTCCAGGACTTCACTGGAGTACCAGCTGTGATTGACCTTGCATGTATCTGGGATGCCATGGAGGAGCTTGGAAATGATCCCAACAAGATAAATCCACTGGTTCCTGTAGACCTTGTTGTCAATCATTCGGTTACAGTTTATGTAGCAAGAAGCCAAGAACTAAATAAAGAGAGATTTGCTTTCCTTAAATGGGGATCTTTTGCATTCCATAACATGCTGCTCCTGGTTAATCTGGAATACCTTGCCAGGTTTGTTTTCGAAACTGATGGAATTCTATACCCTGATAGCGTGGTTGATACCGATTCCCACACAACCATGATTGATAGGGTTAGGAGTTGCTTGCTGGGAGTGGGTGGAATTGAAGCTGAGGCAACAATTAATGCTTGCCTATTAGCATGGTCTTACCTGGTGTTGTTGGGTTCAAGCTTACTGGGCAACTGCGTGAAGGTGTCACTGCTACCGGCCGACTTAGTTTTAACTGTGACACAAATGTTGAAGAAACATGGTGTTGTTGGCAAGTTTGTCGAGTTCTATGGTGAGGGCATGGGTGAAATATCACTCGCTGACAGGGCCACAATTGCAAACATGTCTCCTGAATATGGAGCAACCATGGGCTTCTTTCCTGTAGATCATGTTACATTGCAGTATCTTAAACTGACTGGAAGAAGTGATGAGAAGGTTGCAATGATAGAATCATACTTGAGGGCAAACAAAATGTTCATCGACTACAATGAGCCTCAACAAGAAACAGAATACACATCTCATCTACAGTTAGACCTTGCAGATGTTGAACCCTGTGTTTCAGGACCAAAACGACCTCATGACCGAGTTCCTTTAAAGGACATGAAGGCTGATTGGCATGCATGCCTAAATAGCAAAGTTGGATTCAAAGGTTTTGATGTGCCAAAAGAGAAACAAGGTGAAGTTGCAAAGTTTTTGTTTCATGGACAACCTGCCGAACTTACGCATGGCAGCTTTGTCATTGCAGCAGTTACAAGTTGTACAAACACATCTAATCCCACCGTAATGCTTGGGGCTGGACTAGTTGCGAAGAAAGCTTGTGAACTAGGCTTGGAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230
Pfam Domains
Protein Families
Aconitase Aconitase_C

Protein Analysis

438

Amino Acids

48.38

Weight (kDa)

5.43

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aconitase PF00330 60 - 438 9.2e-87 Aconitase family (aconitate hydratase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018719)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26970
malus_domestica MD03G1199800.v1.1
rosa_multiflora Rmu_sc0000103.1_g000062
rosa_roxburghii Rroxscaffold_1G00041170
rosa_samantha Rh5AG270700 Rh5BG273700 Rh5BG273800 Rh5CG306800
rosa_wichuraiana Rw5G025260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
Acc36I ACCTGC 1 cut(s) 1195
AccB7I CCANNNNNTGG 1 cut(s) 50
AccI GTMKAC 1 cut(s) 342
AclWI GGATC 2 cut(s) 305, 436
AcoI YGGCCR 1 cut(s) 696
AcsI RAATTY 3 cut(s) 62, 71, 504
AfaI GTAC 2 cut(s) 255, 1237
AfiI CCNNNNNNNGG 4 cut(s) 50, 56, 424, 833
AflIII ACRYGT 1 cut(s) 819
AgsI TTSAA 8 cut(s) 96, 164, 206, 596, 657, 730, 1025, 1123
AhlI ACTAGT 1 cut(s) 1276
AjnI CCWGG 5 cut(s) 49, 237, 456, 479, 639
AluBI AGCT 6 cut(s) 260, 301, 599, 660, 1209, 1292
AluI AGCT 6 cut(s) 260, 301, 599, 660, 1209, 1292
Alw26I GTCTC 2 cut(s) 44, 828
AlwI GGATC 2 cut(s) 305, 436
AoxI GGCC 2 cut(s) 696, 804
ApeKI GCWGC 3 cut(s) 451, 1206, 1220
ApoI RAATTY 3 cut(s) 62, 71, 504
AseI ATTAAT 1 cut(s) 612
AspS9I GGNCC 2 cut(s) 804, 1040
AsuHPI GGTGA 4 cut(s) 183, 782, 794, 1166
AsuII TTCGAA 1 cut(s) 492
AvaII GGWCC 1 cut(s) 1040
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BauI CACGAG 1 cut(s) 225
BbvCI CCTCAGC 1 cut(s) 600
BbvI GCAGC 3 cut(s) 438, 1218, 1232
BccI CCATC 2 cut(s) 163, 494
BcgI CGANNNNNNTGC 2 cut(s) 739, 773
BciT130I CCWGG 5 cut(s) 51, 239, 458, 481, 641
BcoDI GTCTC 2 cut(s) 44, 828
BcuI ACTAGT 1 cut(s) 1276
BfaI CTAG 2 cut(s) 1277, 1301
BfmI CTRYAG 3 cut(s) 339, 858, 1001
BfuAI ACCTGC 1 cut(s) 1195
BisI GCNGC 3 cut(s) 452, 1207, 1221
BlsI GCNGC 3 cut(s) 453, 1208, 1222
Bme1390I CCNGG 5 cut(s) 51, 239, 458, 481, 641
Bme18I GGWCC 1 cut(s) 1040
BmgT120I GGNCC 2 cut(s) 804, 1040
BmiI GGNNCC 1 cut(s) 336
BmrFI CCNGG 5 cut(s) 51, 239, 458, 481, 641
BmrI ACTGGG 1 cut(s) 674
BmsI GCATC 1 cut(s) 277
BmuI ACTGGG 1 cut(s) 674
BpmI CTGGAG 3 cut(s) 72, 221, 270
Bpu10I CCTNAGC 1 cut(s) 600
Bpu14I TTCGAA 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 955
BsaJI CCNNGG 3 cut(s) 150, 291, 843
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 56, 424, 833
Bse118I RCCGGY 1 cut(s) 694
Bse1I ACTGG 4 cut(s) 253, 336, 669, 898
Bse3DI GCAATG 3 cut(s) 872, 924, 1215
BseBI CCWGG 5 cut(s) 51, 239, 458, 481, 641
BseDI CCNNGG 3 cut(s) 150, 291, 843
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 4 cut(s) 50, 56, 424, 833
BseMI GCAATG 3 cut(s) 872, 924, 1215
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 4 cut(s) 253, 336, 669, 898
BseRI GAGGAG 2 cut(s) 224, 311
BseX3I CGGCCG 1 cut(s) 696
BseXI GCAGC 3 cut(s) 438, 1218, 1232
BseYI CCCAGC 1 cut(s) 577
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 2 cut(s) 698, 806
BsiEI CGRYCG 1 cut(s) 699
BsiSI CCGG 1 cut(s) 695
BslI CCNNNNNNNGG 4 cut(s) 50, 56, 424, 833
BsmAI GTCTC 2 cut(s) 44, 828
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 2 cut(s) 698, 806
Bsp119I TTCGAA 1 cut(s) 492
Bsp1407I TGTACA 1 cut(s) 1235
Bsp143I GATC 3 cut(s) 310, 428, 862
Bsp19I CCATGG 2 cut(s) 291, 843
BspANI GGCC 2 cut(s) 698, 806
BspCNI CTCAG 1 cut(s) 592
BspHI TCATGA 1 cut(s) 1054
BspLI GGNNCC 1 cut(s) 336
BspMI ACCTGC 1 cut(s) 1195
BspPI GGATC 2 cut(s) 305, 436
BspT104I TTCGAA 1 cut(s) 492
BsrDI GCAATG 3 cut(s) 872, 924, 1215
BsrFI RCCGGY 1 cut(s) 694
BsrGI TGTACA 1 cut(s) 1235
BsrI ACTGG 4 cut(s) 253, 336, 669, 898
BssAI RCCGGY 1 cut(s) 694
BssECI CCNNGG 3 cut(s) 150, 291, 843
BssMI GATC 3 cut(s) 310, 428, 862
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 3 cut(s) 150, 291, 843
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 5 cut(s) 51, 239, 458, 481, 641
Bst4CI ACNGT 6 cut(s) 38, 131, 370, 715, 1005, 1258
BstAUI TGTACA 1 cut(s) 1235
BstBI TTCGAA 1 cut(s) 492
BstC8I GCNNGC 5 cut(s) 222, 575, 619, 1095, 1099
BstDEI CTNAG 2 cut(s) 600, 703
BstDSI CCRYGG 2 cut(s) 291, 843
BstF5I GGATG 1 cut(s) 292
BstKTI GATC 3 cut(s) 313, 431, 865
BstMAI GTCTC 2 cut(s) 44, 828
BstMBI GATC 3 cut(s) 310, 428, 862
BstMCI CGRYCG 1 cut(s) 699
BstMWI GCNNNNNNNGC 5 cut(s) 666, 803, 812, 1107, 1289
BstNI CCWGG 5 cut(s) 51, 239, 458, 481, 641
BstNSI RCATGY 6 cut(s) 17, 279, 451, 823, 1097, 1101
BstSCI CCNGG 5 cut(s) 49, 237, 456, 479, 639
BstSFI CTRYAG 3 cut(s) 339, 858, 1001
BstV1I GCAGC 3 cut(s) 438, 1218, 1232
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BstZI CGGCCG 1 cut(s) 696
BsuRI GGCC 2 cut(s) 698, 806
BtgI CCRYGG 2 cut(s) 291, 843
BtsCI GGATG 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 686
BtsIMutI CAGTG 3 cut(s) 246, 329, 686
BveI ACCTGC 1 cut(s) 1195
Cac8I GCNNGC 5 cut(s) 222, 575, 619, 1095, 1099
CciI TCATGA 1 cut(s) 1054
Cfr10I RCCGGY 1 cut(s) 694
Cfr13I GGNCC 2 cut(s) 804, 1040
CsiI ACCWGGT 1 cut(s) 639
Csp6I GTAC 2 cut(s) 254, 1236
CviQI GTAC 2 cut(s) 254, 1236
DdeI CTNAG 2 cut(s) 600, 703
DpnI GATC 3 cut(s) 312, 430, 864
DpnII GATC 3 cut(s) 310, 428, 862
DraI TTTAAA 1 cut(s) 1071
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 1 cut(s) 696
EagI CGGCCG 1 cut(s) 696
EclXI CGGCCG 1 cut(s) 696
Eco130I CCWWGG 3 cut(s) 150, 291, 843
Eco47I GGWCC 1 cut(s) 1040
Eco52I CGGCCG 1 cut(s) 696
EcoRI GAATTC 1 cut(s) 504
EcoRII CCWGG 5 cut(s) 49, 237, 456, 479, 639
EcoT14I CCWWGG 3 cut(s) 150, 291, 843
EcoT22I ATGCAT 1 cut(s) 1099
ErhI CCWWGG 3 cut(s) 150, 291, 843
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 3 cut(s) 452, 1207, 1221
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 3 cut(s) 452, 1207, 1221
FspBI CTAG 2 cut(s) 1277, 1301
GluI GCNGC 3 cut(s) 452, 1207, 1221
GsaI CCCAGC 1 cut(s) 581
GsuI CTGGAG 3 cut(s) 72, 221, 270
HaeIII GGCC 2 cut(s) 698, 806
HapII CCGG 1 cut(s) 695
HindIII AAGCTT 2 cut(s) 658, 1290
HinfI GANTC 4 cut(s) 113, 536, 926, 1119
HpaII CCGG 1 cut(s) 695
HphI GGTGA 4 cut(s) 183, 782, 794, 1166
Hpy166II GTNNAC 2 cut(s) 343, 1298
Hpy188I TCNGA 1 cut(s) 68
Hpy188III TCNNGA 4 cut(s) 467, 827, 1038, 1055
Hpy8I GTNNAC 2 cut(s) 343, 1298
HpyAV CCTTC 3 cut(s) 673, 904, 1075
HpyCH4III ACNGT 6 cut(s) 38, 131, 370, 715, 1005, 1258
HpyF10VI GCNNNNNNNGC 5 cut(s) 666, 803, 812, 1107, 1289
HpyF3I CTNAG 2 cut(s) 600, 703
Kzo9I GATC 3 cut(s) 310, 428, 862
LmnI GCTCC 3 cut(s) 298, 459, 836
Lsp1109I GCAGC 3 cut(s) 438, 1218, 1232
LweI GCATC 1 cut(s) 277
MabI ACCWGGT 1 cut(s) 639
MaeI CTAG 2 cut(s) 1277, 1301
MaeIII GTNAC 6 cut(s) 131, 364, 684, 715, 868, 1225
MalI GATC 3 cut(s) 312, 430, 864
MboI GATC 3 cut(s) 310, 428, 862
MboII GAAGA 4 cut(s) 167, 742, 909, 1297
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 428
MluCI AATT 7 cut(s) 62, 71, 144, 504, 591, 609, 810
MmeI TCCRAC 1 cut(s) 1096
Mph1103I ATGCAT 1 cut(s) 1099
MseI TTAA 8 cut(s) 32, 216, 420, 462, 612, 710, 885, 1070
MslI CAYNNNNRTG 3 cut(s) 129, 548, 1259
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 1 cut(s) 695
MspR9I CCNGG 5 cut(s) 51, 239, 458, 481, 641
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 5 cut(s) 51, 239, 458, 481, 641
MwoI GCNNNNNNNGC 5 cut(s) 666, 803, 812, 1107, 1289
NcoI CCATGG 2 cut(s) 291, 843
NdeII GATC 3 cut(s) 310, 428, 862
NlaIV GGNNCC 1 cut(s) 336
NmuCI GTSAC 3 cut(s) 131, 684, 715
NsiI ATGCAT 1 cut(s) 1099
NspI RCATGY 6 cut(s) 17, 279, 451, 823, 1097, 1101
NspV TTCGAA 1 cut(s) 492
PaeI GCATGC 2 cut(s) 1097, 1101
PagI TCATGA 1 cut(s) 1054
PciI ACATGT 1 cut(s) 819
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 4 cut(s) 113, 536, 926, 1119
PflMI CCANNNNNTGG 1 cut(s) 50
PfoI TCCNGGA 1 cut(s) 237
PkrI GCNGC 3 cut(s) 453, 1208, 1222
PscI ACATGT 1 cut(s) 819
PshBI ATTAAT 1 cut(s) 612
Psp6I CCWGG 5 cut(s) 49, 237, 456, 479, 639
PspFI CCCAGC 1 cut(s) 577
PspGI CCWGG 5 cut(s) 49, 237, 456, 479, 639
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 2 cut(s) 804, 1040
PsuI RGATCY 1 cut(s) 428
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 2 cut(s) 255, 1237
RsaNI GTAC 2 cut(s) 254, 1236
RseI CAYNNNNRTG 3 cut(s) 129, 548, 1259
SaqAI TTAA 8 cut(s) 32, 216, 420, 462, 612, 710, 885, 1070
SatI GCNGC 3 cut(s) 452, 1207, 1221
Sau3AI GATC 3 cut(s) 310, 428, 862
Sau96I GGNCC 2 cut(s) 804, 1040
ScrFI CCNGG 5 cut(s) 51, 239, 458, 481, 641
SexAI ACCWGGT 1 cut(s) 639
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 3 cut(s) 339, 858, 1001
SfuI TTCGAA 1 cut(s) 492
SinI GGWCC 1 cut(s) 1040
SmiMI CAYNNNNRTG 3 cut(s) 129, 548, 1259
SmlI CTYRAG 1 cut(s) 934
SmoI CTYRAG 1 cut(s) 934
SpeI ACTAGT 1 cut(s) 1276
SphI GCATGC 2 cut(s) 1097, 1101
Sse9I AATT 7 cut(s) 62, 71, 144, 504, 591, 609, 810
SspI AATATT 1 cut(s) 30
SspMI CTAG 2 cut(s) 1277, 1301
StyD4I CCNGG 5 cut(s) 49, 237, 456, 479, 639
StyI CCWWGG 3 cut(s) 150, 291, 843
TaaI ACNGT 6 cut(s) 38, 131, 370, 715, 1005, 1258
TaqI TCGA 3 cut(s) 492, 759, 957
TaqII GACCGA 1 cut(s) 1074
TasI AATT 7 cut(s) 62, 71, 144, 504, 591, 609, 810
TatI WGTACW 1 cut(s) 1235
TfiI GAWTC 4 cut(s) 113, 536, 926, 1119
Tru1I TTAA 8 cut(s) 32, 216, 420, 462, 612, 710, 885, 1070
Tru9I TTAA 8 cut(s) 32, 216, 420, 462, 612, 710, 885, 1070
TscAI CASTG 3 cut(s) 253, 336, 693
TseFI GTSAC 3 cut(s) 131, 684, 715
TseI GCWGC 3 cut(s) 451, 1206, 1220
Tsp45I GTSAC 3 cut(s) 131, 684, 715
TspDTI ATGAA 3 cut(s) 943, 1094, 1166
TspRI CASTG 3 cut(s) 253, 336, 693
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 1040
VspI ATTAAT 1 cut(s) 612
XapI RAATTY 3 cut(s) 62, 71, 504
XceI RCATGY 6 cut(s) 17, 279, 451, 823, 1097, 1101
XcmI CCANNNNNNNNNTGG 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 2 cut(s) 1277, 1301
Zsp2I ATGCAT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.