Rh5CG306800

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0018719
Physical Location & Seq
Reverse (-)
33833729 .. 33835116
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG306800.1

Sequence Viewer

Length: 576 bp
ATGTTTGTTTCCATAGTGCCTCTCTCTTCCTCGCTCTCCATAATCTCACCGACGTCGTTTTTGCACCGCTCTCTCAGCTTCTTGTCCGCTCTTCCGTCTCCGGGCTACTCCGCCGTGCTCCGGAACCACCGACTCTATTGGAAATCTCCGGTTAGCTTCCTTGCTCATCAAACAACTGCCCCACCACTCATCAACCACCGCTTCCGGCGAAACATGGCCGCCACTGCTTCTCAACATGCTTTTGAGGGAATATTAAACAGTCTCCCAAAGCCTGGAGGTGGCGAATTTGGAAAATTTTACAGTCTACCTGCTCTAAATGATCCAAGAATTGACAGGCTTCCATATTCAATAAAGATACTTTTGGAATCTGCAATCCGTAACTGTGACAACTTCCAAATTATCAAGGAAGATGTTGAAAAGATAGCTGATTGGGAGAATACATCACCAAAGCAAGTTGAAATACCCTTTAAGCCCGCTCGTGTTCTCCTCCAGGACTTCACTGGAGTACCAGCTGTGATTGACCTTGCATGTATCCGGGATGCCATGGAGGAGCTGGAAATGATCCCAACAAAATAA

Protein Analysis

191

Amino Acids

21.36

Weight (kDa)

8.58

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018719)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26970
malus_domestica MD03G1199800.v1.1
rosa_multiflora Rmu_sc0000103.1_g000062
rosa_roxburghii Rroxscaffold_1G00041170
rosa_samantha Rh5AG270700 Rh5BG273700 Rh5BG273800 Rh5CG306800
rosa_wichuraiana Rw5G025260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 56
Acc36I ACCTGC 1 cut(s) 316
AccB7I CCANNNNNTGG 1 cut(s) 272
AccBSI CCGCTC 3 cut(s) 69, 89, 476
AccI GTMKAC 1 cut(s) 304
AccIII TCCGGA 1 cut(s) 120
AciI CCGC 6 cut(s) 67, 87, 111, 199, 219, 474
AclWI GGATC 2 cut(s) 314, 556
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 2 cut(s) 284, 293
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 1 cut(s) 507
AfiI CCNNNNNNNGG 4 cut(s) 101, 120, 272, 278
AgsI TTSAA 3 cut(s) 348, 416, 458
AjnI CCWGG 2 cut(s) 271, 489
AluBI AGCT 5 cut(s) 78, 156, 425, 512, 553
AluI AGCT 5 cut(s) 78, 156, 425, 512, 553
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 2 cut(s) 102, 266
AlwI GGATC 2 cut(s) 314, 556
Aor13HI TCCGGA 1 cut(s) 120
AoxI GGCC 1 cut(s) 216
ApoI RAATTY 2 cut(s) 284, 293
ArsI GACNNNNNNTTYG 2 cut(s) 43, 75
AsuC2I CCSGG 2 cut(s) 102, 536
AsuHPI GGTGA 2 cut(s) 39, 435
BaeI ACNNNNGTAYC 2 cut(s) 489, 522
BauI CACGAG 1 cut(s) 477
Bbv12I GWGCWC 1 cut(s) 120
BceAI ACGGC 1 cut(s) 98
BciT130I CCWGG 2 cut(s) 273, 491
BciVI GTATCC 1 cut(s) 542
BcnI CCSGG 2 cut(s) 102, 536
BcoDI GTCTC 2 cut(s) 102, 266
BfuAI ACCTGC 1 cut(s) 316
BfuI GTATCC 1 cut(s) 542
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
Bme1390I CCNGG 4 cut(s) 102, 273, 491, 536
BmiI GGNNCC 1 cut(s) 125
BmrFI CCNGG 4 cut(s) 102, 273, 491, 536
BmsI GCATC 1 cut(s) 529
BpmI CTGGAG 3 cut(s) 294, 473, 522
BpuMI CCSGG 2 cut(s) 102, 536
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 543
BsaWI WCCGGW 2 cut(s) 120, 148
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 120, 272, 278
Bse1I ACTGG 1 cut(s) 505
BseAI TCCGGA 1 cut(s) 120
BseBI CCWGG 2 cut(s) 273, 491
BseDI CCNNGG 1 cut(s) 543
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 4 cut(s) 101, 120, 272, 278
BseMII CTCAG 1 cut(s) 88
BseNI ACTGG 1 cut(s) 505
BseRI GAGGAG 2 cut(s) 476, 563
BshFI GGCC 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 5 cut(s) 101, 121, 149, 205, 535
BslI CCNNNNNNNGG 4 cut(s) 101, 120, 272, 278
BsmAI GTCTC 2 cut(s) 102, 266
BsmBI CGTCTC 1 cut(s) 102
BsnI GGCC 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 120
Bsp13I TCCGGA 1 cut(s) 120
Bsp143I GATC 2 cut(s) 319, 561
Bsp19I CCATGG 1 cut(s) 543
BspACI CCGC 6 cut(s) 67, 87, 111, 199, 219, 474
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 1 cut(s) 87
BspEI TCCGGA 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 125
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 2 cut(s) 314, 556
BspQI GCTCTTC 1 cut(s) 96
BsrBI CCGCTC 3 cut(s) 69, 89, 476
BsrI ACTGG 1 cut(s) 505
BssECI CCNNGG 1 cut(s) 543
BssMI GATC 2 cut(s) 319, 561
BssNI GRCGYC 1 cut(s) 53
BssSI CACGAG 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 543
Bst2BI CACGAG 1 cut(s) 477
Bst2UI CCWGG 2 cut(s) 273, 491
Bst4CI ACNGT 3 cut(s) 260, 302, 383
Bst6I CTCTTC 2 cut(s) 31, 96
BstACI GRCGYC 1 cut(s) 53
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 1 cut(s) 74
BstDSI CCRYGG 1 cut(s) 543
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 2 cut(s) 322, 564
BstMAI GTCTC 2 cut(s) 102, 266
BstMBI GATC 2 cut(s) 319, 561
BstMWI GCNNNNNNNGC 2 cut(s) 75, 224
BstNI CCWGG 2 cut(s) 273, 491
BstNSI RCATGY 2 cut(s) 239, 531
BstSCI CCNGG 4 cut(s) 100, 271, 489, 534
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 1 cut(s) 218
BtgI CCRYGG 1 cut(s) 543
BtsCI GGATG 1 cut(s) 544
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 222, 498
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 474
Csp6I GTAC 1 cut(s) 506
CviAII CATG 4 cut(s) 214, 236, 528, 544
CviQI GTAC 1 cut(s) 506
DdeI CTNAG 1 cut(s) 74
DpnI GATC 2 cut(s) 321, 563
DpnII GATC 2 cut(s) 319, 561
EaeI YGGCCR 1 cut(s) 216
Eam1104I CTCTTC 2 cut(s) 31, 96
EarI CTCTTC 2 cut(s) 31, 96
EciI GGCGGA 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 543
EcoRII CCWGG 2 cut(s) 271, 489
EcoT14I CCWWGG 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 543
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 4 cut(s) 217, 239, 531, 547
FaiI YATR 7 cut(s) 14, 41, 215, 237, 343, 529, 545
FatI CATG 4 cut(s) 213, 235, 527, 543
FauI CCCGC 1 cut(s) 481
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 219
GsuI CTGGAG 3 cut(s) 294, 473, 522
HaeIII GGCC 1 cut(s) 218
HapII CCGG 5 cut(s) 101, 121, 149, 205, 535
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 4 cut(s) 217, 239, 531, 547
HinfI GANTC 2 cut(s) 132, 365
HpaII CCGG 5 cut(s) 101, 121, 149, 205, 535
HphI GGTGA 2 cut(s) 39, 435
Hpy166II GTNNAC 1 cut(s) 305
Hpy188III TCNNGA 1 cut(s) 121
Hpy8I GTNNAC 1 cut(s) 305
Hpy99I CGWCG 2 cut(s) 55, 58
HpyCH4III ACNGT 3 cut(s) 260, 302, 383
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 3 cut(s) 64, 371, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 224
HpyF3I CTNAG 1 cut(s) 74
HpySE526I ACGT 1 cut(s) 53
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 4 cut(s) 217, 239, 531, 547
Kpn2I TCCGGA 1 cut(s) 120
Kzo9I GATC 2 cut(s) 319, 561
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 2 cut(s) 123, 550
LweI GCATC 1 cut(s) 529
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 2 cut(s) 377, 383
MalI GATC 2 cut(s) 321, 563
MbiI CCGCTC 3 cut(s) 69, 89, 476
MboI GATC 2 cut(s) 319, 561
MboII GAAGA 3 cut(s) 18, 83, 419
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 4 cut(s) 284, 293, 327, 396
MlyI GAGTC 1 cut(s) 126
MnlI CCTC 6 cut(s) 30, 40, 238, 269, 497, 541
MroI TCCGGA 1 cut(s) 120
MseI TTAA 2 cut(s) 254, 468
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 5 cut(s) 101, 121, 149, 205, 535
MspR9I CCNGG 4 cut(s) 102, 273, 491, 536
MvaI CCWGG 2 cut(s) 273, 491
MwoI GCNNNNNNNGC 2 cut(s) 75, 224
NciI CCSGG 2 cut(s) 102, 536
NcoI CCATGG 1 cut(s) 543
NdeII GATC 2 cut(s) 319, 561
NlaIII CATG 4 cut(s) 217, 239, 531, 547
NlaIV GGNNCC 1 cut(s) 125
NmuCI GTSAC 1 cut(s) 383
NspI RCATGY 2 cut(s) 239, 531
PciSI GCTCTTC 1 cut(s) 96
PfeI GAWTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 272
PfoI TCCNGGA 2 cut(s) 489, 534
PkrI GCNGC 1 cut(s) 220
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 271, 489
PspGI CCWGG 2 cut(s) 271, 489
PspN4I GGNNCC 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 2 cut(s) 254, 468
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 2 cut(s) 319, 561
SchI GAGTC 1 cut(s) 126
ScrFI CCNGG 4 cut(s) 102, 273, 491, 536
SduI GDGCHC 1 cut(s) 120
SetI ASST 9 cut(s) 56, 80, 158, 280, 310, 427, 514, 525, 555
SfaNI GCATC 1 cut(s) 529
Sse9I AATT 4 cut(s) 284, 293, 327, 396
SsiI CCGC 6 cut(s) 67, 87, 111, 199, 219, 474
SspI AATATT 1 cut(s) 252
StyD4I CCNGG 4 cut(s) 100, 271, 489, 534
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 3 cut(s) 260, 302, 383
TaiI ACGT 1 cut(s) 56
TasI AATT 4 cut(s) 284, 293, 327, 396
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 2 cut(s) 254, 468
Tru9I TTAA 2 cut(s) 254, 468
TscAI CASTG 2 cut(s) 229, 505
TseFI GTSAC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 383
TspGWI ACGGA 2 cut(s) 84, 365
TspRI CASTG 2 cut(s) 229, 505
Van91I CCANNNNNTGG 1 cut(s) 272
XapI RAATTY 2 cut(s) 284, 293
XceI RCATGY 2 cut(s) 239, 531
XcmI CCANNNNNNNNNTGG 2 cut(s) 497, 550
XmiI GTMKAC 1 cut(s) 304
ZraI GACGTC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.