Rh5AG270700

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018719
Physical Location & Seq
Reverse (-)
35879596 .. 35887845
8250 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG270700.1

Sequence Viewer

Length: 591 bp
ATGGCCGCCACTGCTTCTCAACATGCTTTTGAGGGAATATTAAACAGTCTCCCAAAGCCTGGAGGTGGCGAATTCGGAAAATTTTACAGTCTACCTGCTCTAATTGATCCAAGAATTGACAGGCTTCCATATTCAATAAAGATACTTTTGGAATCTGCAATCCGTAACTGTGACAACTTCCAAATTACCAAGGAAGATGTTGAAAAGATAGCTGATTGGGAGAATGCATCACCAAAGCAAGTTGAAATACCCTTTAAGCCCGCTCGTGTTCTCCTCCAGGACTTCACTGGAGTACCAGCTGTGATTGACCTTGCATGTATCCGGGATGCCATGGAGGAGCTTGGAAATGATCCCAACAAGATAAATCCATTGGTTCCTGTAGACCTTGTTGTTGATCATTCGGTTACAGTTGATGTAGCAAGAACTGAAGGTGCACTACAGGAAAATATGGAACGAGAGTTTCAGAGAAATAAAGAGAGATTTGCTTTCCTTAAATGGGGATCTTTTGCATTCCATAACATGCTTGTAGTACCTCCTGGTTCTGGAATTGTCCATCAGGTTAATCTGGAATACCTTGCCAGGGTTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230
Pfam Domains
Protein Families
Aconitase Aconitase_C

Protein Analysis

196

Amino Acids

21.87

Weight (kDa)

5.13

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aconitase PF00330 69 - 192 3.1e-29 Aconitase family (aconitate hydratase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018719)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26970
malus_domestica MD03G1199800.v1.1
rosa_multiflora Rmu_sc0000103.1_g000062
rosa_roxburghii Rroxscaffold_1G00041170
rosa_samantha Rh5AG270700 Rh5BG273700 Rh5BG273800 Rh5CG306800
rosa_wichuraiana Rw5G025260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 103
AccB7I CCANNNNNTGG 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 263
AccI GTMKAC 2 cut(s) 91, 381
AciI CCGC 2 cut(s) 6, 261
AclWI GGATC 3 cut(s) 101, 344, 508
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 71, 80
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 2 cut(s) 294, 531
AfiI CCNNNNNNNGG 5 cut(s) 59, 65, 496, 542, 580
AgsI TTSAA 3 cut(s) 135, 203, 245
AjnI CCWGG 4 cut(s) 58, 276, 535, 578
AluBI AGCT 3 cut(s) 212, 299, 340
AluI AGCT 3 cut(s) 212, 299, 340
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 1 cut(s) 53
Alw44I GTGCAC 1 cut(s) 432
AlwI GGATC 3 cut(s) 101, 344, 508
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 432
ApoI RAATTY 2 cut(s) 71, 80
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 222
BaeGI GKGCMC 1 cut(s) 436
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BauI CACGAG 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 436
BccI CCATC 1 cut(s) 561
BciT130I CCWGG 4 cut(s) 60, 278, 537, 580
BciVI GTATCC 1 cut(s) 329
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 53
BfmI CTRYAG 2 cut(s) 378, 437
BfuAI ACCTGC 1 cut(s) 103
BfuI GTATCC 1 cut(s) 329
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 5 cut(s) 60, 278, 323, 537, 580
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 5 cut(s) 60, 278, 323, 537, 580
BmsI GCATC 2 cut(s) 236, 316
BpmI CTGGAG 3 cut(s) 81, 260, 309
BpuMI CCSGG 1 cut(s) 323
BsaJI CCNNGG 3 cut(s) 189, 330, 579
Bsc4I CCNNNNNNNGG 5 cut(s) 59, 65, 496, 542, 580
Bse1I ACTGG 1 cut(s) 292
BseBI CCWGG 4 cut(s) 60, 278, 537, 580
BseDI CCNNGG 3 cut(s) 189, 330, 579
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 5 cut(s) 59, 65, 496, 542, 580
BseNI ACTGG 1 cut(s) 292
BseRI GAGGAG 2 cut(s) 263, 350
BseSI GKGCMC 1 cut(s) 436
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 1 cut(s) 322
BslI CCNNNNNNNGG 5 cut(s) 59, 65, 496, 542, 580
BsmAI GTCTC 1 cut(s) 53
BsmI GAATGC 2 cut(s) 229, 509
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 4 cut(s) 106, 349, 394, 500
Bsp19I CCATGG 1 cut(s) 330
BspACI CCGC 2 cut(s) 6, 261
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 1 cut(s) 375
BspMI ACCTGC 1 cut(s) 103
BspPI GGATC 3 cut(s) 101, 344, 508
BsrBI CCGCTC 1 cut(s) 263
BsrI ACTGG 1 cut(s) 292
BssECI CCNNGG 3 cut(s) 189, 330, 579
BssMI GATC 4 cut(s) 106, 349, 394, 500
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 2 cut(s) 189, 330
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 4 cut(s) 60, 278, 537, 580
Bst4CI ACNGT 4 cut(s) 47, 89, 170, 409
BstC8I GCNNGC 1 cut(s) 261
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 4 cut(s) 109, 352, 397, 503
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 4 cut(s) 106, 349, 394, 500
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 4 cut(s) 60, 278, 537, 580
BstNSI RCATGY 3 cut(s) 26, 318, 523
BstSCI CCNGG 5 cut(s) 58, 276, 321, 535, 578
BstSFI CTRYAG 2 cut(s) 378, 437
BstSLI GKGCMC 1 cut(s) 436
BstX2I RGATCY 1 cut(s) 500
BstYI RGATCY 1 cut(s) 500
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 331
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 2 cut(s) 9, 285
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 261
Csp6I GTAC 2 cut(s) 293, 530
CviAII CATG 4 cut(s) 23, 315, 331, 520
CviJI RGCY 7 cut(s) 5, 58, 124, 212, 259, 299, 340
CviKI_1 RGCY 7 cut(s) 5, 58, 124, 212, 259, 299, 340
CviQI GTAC 2 cut(s) 293, 530
DpnI GATC 4 cut(s) 108, 351, 396, 502
DpnII GATC 4 cut(s) 106, 349, 394, 500
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 189, 330
Eco57I CTGAAG 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 4 cut(s) 58, 276, 535, 578
EcoT14I CCWWGG 2 cut(s) 189, 330
EcoT22I ATGCAT 1 cut(s) 229
ErhI CCWWGG 2 cut(s) 189, 330
FaeI CATG 4 cut(s) 26, 318, 334, 523
FaiI YATR 7 cut(s) 24, 130, 316, 332, 449, 516, 521
FatI CATG 4 cut(s) 22, 314, 330, 519
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 2 cut(s) 91, 381
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 338
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 3 cut(s) 81, 260, 309
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 322
Hin1II CATG 4 cut(s) 26, 318, 334, 523
HinfI GANTC 1 cut(s) 152
HpaII CCGG 1 cut(s) 322
HphI GGTGA 1 cut(s) 222
Hpy166II GTNNAC 3 cut(s) 92, 382, 434
Hpy188I TCNGA 3 cut(s) 77, 465, 590
Hpy188III TCNNGA 2 cut(s) 543, 566
Hpy8I GTNNAC 3 cut(s) 92, 382, 434
HpyAV CCTTC 1 cut(s) 422
HpyCH4III ACNGT 4 cut(s) 47, 89, 170, 409
HpyCH4V TGCA 5 cut(s) 158, 227, 314, 434, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
Hsp92II CATG 4 cut(s) 26, 318, 334, 523
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 4 cut(s) 106, 349, 394, 500
LmnI GCTCC 1 cut(s) 337
LweI GCATC 2 cut(s) 236, 316
MaeIII GTNAC 3 cut(s) 164, 170, 403
MalI GATC 4 cut(s) 108, 351, 396, 502
MbiI CCGCTC 1 cut(s) 263
MboI GATC 4 cut(s) 106, 349, 394, 500
MboII GAAGA 1 cut(s) 206
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 6 cut(s) 71, 80, 102, 114, 183, 546
MnlI CCTC 5 cut(s) 25, 56, 284, 328, 543
Mph1103I ATGCAT 1 cut(s) 229
MseI TTAA 4 cut(s) 41, 255, 492, 561
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 5 cut(s) 60, 278, 323, 537, 580
Mva1269I GAATGC 2 cut(s) 229, 509
MvaI CCWGG 4 cut(s) 60, 278, 537, 580
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 323
NcoI CCATGG 1 cut(s) 330
NdeII GATC 4 cut(s) 106, 349, 394, 500
NlaIII CATG 4 cut(s) 26, 318, 334, 523
NlaIV GGNNCC 1 cut(s) 375
NmuCI GTSAC 1 cut(s) 170
NsiI ATGCAT 1 cut(s) 229
NspI RCATGY 3 cut(s) 26, 318, 523
PctI GAATGC 2 cut(s) 229, 509
PfeI GAWTC 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 59
PfoI TCCNGGA 2 cut(s) 276, 321
PkrI GCNGC 1 cut(s) 7
Psp6I CCWGG 4 cut(s) 58, 276, 535, 578
PspGI CCWGG 4 cut(s) 58, 276, 535, 578
PspN4I GGNNCC 1 cut(s) 375
PsuI RGATCY 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 2 cut(s) 294, 531
RsaNI GTAC 2 cut(s) 293, 530
SaqAI TTAA 4 cut(s) 41, 255, 492, 561
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 4 cut(s) 106, 349, 394, 500
ScrFI CCNGG 5 cut(s) 60, 278, 323, 537, 580
SduI GDGCHC 1 cut(s) 436
SfaNI GCATC 2 cut(s) 236, 316
SfcI CTRYAG 2 cut(s) 378, 437
Sse9I AATT 6 cut(s) 71, 80, 102, 114, 183, 546
SsiI CCGC 2 cut(s) 6, 261
SspI AATATT 1 cut(s) 39
StyD4I CCNGG 5 cut(s) 58, 276, 321, 535, 578
StyI CCWWGG 2 cut(s) 189, 330
TaaI ACNGT 4 cut(s) 47, 89, 170, 409
TasI AATT 6 cut(s) 71, 80, 102, 114, 183, 546
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 4 cut(s) 41, 255, 492, 561
Tru9I TTAA 4 cut(s) 41, 255, 492, 561
TscAI CASTG 2 cut(s) 16, 292
TseFI GTSAC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 170
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 2 cut(s) 16, 292
Van91I CCANNNNNTGG 1 cut(s) 59
VneI GTGCAC 1 cut(s) 432
XapI RAATTY 2 cut(s) 71, 80
XceI RCATGY 3 cut(s) 26, 318, 523
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XmiI GTMKAC 2 cut(s) 91, 381
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.