MD03G1199800.v1.1

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0018719
Physical Location & Seq
Forward (+)
27350302 .. 27351889
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1199800.v1.1.491

Sequence Viewer

Length: 645 bp
ATGATCCCAGAATCGCTGCGCTTGATATTATTGTACAGGTTTCCTTATTCTATAAAGATACTCTTGGAATCTGCAATTCGCAACTGTGACAATTTTCAGGTGACCAAGGAGGATGTTGAGAAAATAGTTGATTGGGAGAATACATCGCCAAAGCAAGTCGAGGTTCCATTTAAGCCAGCTCGCGTTCTTCTCCAGGACTTTACTGGAGTACCAGCCGTGATTGACCTTGCATGTATCCGGGATGCCTTGAAGAAGCTTGGAAAGGATCCTAACAAGATAAATCCATTGGTTCCGGTAGATCTTGTCCTTGATCATTCAGTCCAGGTTGATGTAGCAAGAACTGAAAATGCACTTAGGGAAAATATGGAACTTGAATTTGAGAGGAATAAGGAGAGATTTGCTTTCCTCAAATGGGGAGCATCTGCTTTTCATAACATGCTCGTTGTACCTCCCGGTTGTGGAATTGTCCATCAGGTTGGTAGTGGTTCTATTGTGCACAGAAGTATTTGCGCTATTGAAACTTTATGTATCATAAGCCCGAAAGTAAAAACTTCAGAAGCCTTTTTTTTGTTGAGGCCAAGATTTTCCTTGGATGTCTTAAGCCATCAAAATTCACTTCGAAGCTATGAATATTGTATATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230
Pfam Domains
Protein Families
Aconitase Aconitase_C

Protein Analysis

215

Amino Acids

24.39

Weight (kDa)

6.95

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aconitase PF00330 40 - 161 5.5e-27 Aconitase family (aconitate hydratase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018719)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26970
malus_domestica MD03G1199800.v1.1
rosa_multiflora Rmu_sc0000103.1_g000062
rosa_roxburghii Rroxscaffold_1G00041170
rosa_samantha Rh5AG270700 Rh5BG273700 Rh5BG273800 Rh5CG306800
rosa_wichuraiana Rw5G025260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 183
AclWI GGATC 2 cut(s) 260, 273
AcsI RAATTY 2 cut(s) 374, 610
AcuI CTGAAG 1 cut(s) 537
AfaI GTAC 3 cut(s) 35, 210, 447
AfiI CCNNNNNNNGG 2 cut(s) 412, 458
AflII CTTAAG 1 cut(s) 598
AgsI TTSAA 3 cut(s) 250, 374, 518
AjnI CCWGG 2 cut(s) 192, 321
AluBI AGCT 3 cut(s) 179, 256, 624
AluI AGCT 3 cut(s) 179, 256, 624
Alw21I GWGCWC 1 cut(s) 498
Alw44I GTGCAC 1 cut(s) 494
AlwI GGATC 2 cut(s) 260, 273
AoxI GGCC 1 cut(s) 575
ApaLI GTGCAC 1 cut(s) 494
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 2 cut(s) 374, 610
AspLEI GCGC 2 cut(s) 21, 512
AsuC2I CCSGG 2 cut(s) 239, 453
AsuHPI GGTGA 1 cut(s) 112
AsuII TTCGAA 1 cut(s) 619
BaeGI GKGCMC 1 cut(s) 498
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BamHI GGATCC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 1 cut(s) 3
BccI CCATC 2 cut(s) 477, 612
BceAI ACGGC 1 cut(s) 200
BciT130I CCWGG 2 cut(s) 194, 323
BciVI GTATCC 1 cut(s) 245
BclI TGATCA 1 cut(s) 310
BcnI CCSGG 2 cut(s) 239, 453
BfrI CTTAAG 1 cut(s) 598
BfuI GTATCC 1 cut(s) 245
BglII AGATCT 1 cut(s) 298
BisI GCNGC 1 cut(s) 17
BlsI GCNGC 1 cut(s) 18
Bme1390I CCNGG 4 cut(s) 194, 239, 323, 453
BmiI GGNNCC 3 cut(s) 165, 267, 291
BmrFI CCNGG 4 cut(s) 194, 239, 323, 453
BmsI GCATC 2 cut(s) 232, 428
BpmI CTGGAG 2 cut(s) 176, 225
Bpu14I TTCGAA 1 cut(s) 619
BpuMI CCSGG 2 cut(s) 239, 453
BsaJI CCNNGG 2 cut(s) 105, 588
BsaWI WCCGGW 1 cut(s) 292
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 458
Bse1I ACTGG 1 cut(s) 208
BseBI CCWGG 2 cut(s) 194, 323
BseDI CCNNGG 2 cut(s) 105, 588
BseGI GGATG 3 cut(s) 118, 247, 598
BseLI CCNNNNNNNGG 2 cut(s) 412, 458
BseNI ACTGG 1 cut(s) 208
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 1 cut(s) 3
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 1 cut(s) 577
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 3 cut(s) 238, 293, 453
BslI CCNNNNNNNGG 2 cut(s) 412, 458
BsnI GGCC 1 cut(s) 577
Bsp119I TTCGAA 1 cut(s) 619
Bsp1286I GDGCHC 1 cut(s) 498
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 4 cut(s) 3, 265, 298, 310
BspANI GGCC 1 cut(s) 577
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 3 cut(s) 165, 267, 291
BspPI GGATC 2 cut(s) 260, 273
BspT104I TTCGAA 1 cut(s) 619
BspTI CTTAAG 1 cut(s) 598
BsrGI TGTACA 1 cut(s) 33
BsrI ACTGG 1 cut(s) 208
BssECI CCNNGG 2 cut(s) 105, 588
BssMI GATC 4 cut(s) 3, 265, 298, 310
BssT1I CCWWGG 2 cut(s) 105, 588
Bst2UI CCWGG 2 cut(s) 194, 323
Bst4CI ACNGT 1 cut(s) 86
BstAFI CTTAAG 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 33
BstBI TTCGAA 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 177, 181
BstDEI CTNAG 1 cut(s) 353
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 3 cut(s) 118, 247, 598
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 2 cut(s) 21, 512
BstKTI GATC 4 cut(s) 6, 268, 301, 313
BstMBI GATC 4 cut(s) 3, 265, 298, 310
BstNI CCWGG 2 cut(s) 194, 323
BstNSI RCATGY 2 cut(s) 234, 439
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 4 cut(s) 192, 237, 321, 451
BstSLI GKGCMC 1 cut(s) 498
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 3
BstX2I RGATCY 2 cut(s) 265, 298
BstXI CCANNNNNNTGG 1 cut(s) 476
BstYI RGATCY 2 cut(s) 265, 298
BsuI GTATCC 1 cut(s) 245
BsuRI GGCC 1 cut(s) 577
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 3 cut(s) 118, 247, 598
Cac8I GCNNGC 2 cut(s) 177, 181
CfoI GCGC 2 cut(s) 21, 512
Csp6I GTAC 3 cut(s) 34, 209, 446
CviAII CATG 2 cut(s) 231, 436
CviJI RGCY 9 cut(s) 175, 179, 215, 256, 537, 560, 577, 603, 624
CviKI_1 RGCY 9 cut(s) 175, 179, 215, 256, 537, 560, 577, 603, 624
CviQI GTAC 3 cut(s) 34, 209, 446
DdeI CTNAG 1 cut(s) 353
DpnI GATC 4 cut(s) 5, 267, 300, 312
DpnII GATC 4 cut(s) 3, 265, 298, 310
Eco130I CCWWGG 2 cut(s) 105, 588
Eco57I CTGAAG 1 cut(s) 537
Eco91I GGTNACC 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 100
EcoRII CCWGG 2 cut(s) 192, 321
EcoT14I CCWWGG 2 cut(s) 105, 588
ErhI CCWWGG 2 cut(s) 105, 588
FaeI CATG 2 cut(s) 234, 439
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FatI CATG 2 cut(s) 230, 435
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 17
FokI GGATG 3 cut(s) 125, 254, 605
Fsp4HI GCNGC 1 cut(s) 17
GlaI GCGC 2 cut(s) 20, 511
GluI GCNGC 1 cut(s) 17
GsuI CTGGAG 2 cut(s) 176, 225
HaeIII GGCC 1 cut(s) 577
HapII CCGG 3 cut(s) 238, 293, 453
HhaI GCGC 2 cut(s) 21, 512
Hin1II CATG 2 cut(s) 234, 439
Hin6I GCGC 2 cut(s) 19, 510
HinP1I GCGC 2 cut(s) 19, 510
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 2 cut(s) 11, 68
HpaII CCGG 3 cut(s) 238, 293, 453
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 1 cut(s) 556
Hpy8I GTNNAC 1 cut(s) 496
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 4 cut(s) 74, 230, 350, 496
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 2 cut(s) 234, 439
HspAI GCGC 2 cut(s) 19, 510
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 4 cut(s) 3, 265, 298, 310
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 1 cut(s) 3
LweI GCATC 2 cut(s) 232, 428
MaeIII GTNAC 2 cut(s) 86, 100
MalI GATC 4 cut(s) 5, 267, 300, 312
MboI GATC 4 cut(s) 3, 265, 298, 310
MboII GAAGA 2 cut(s) 179, 262
MflI RGATCY 2 cut(s) 265, 298
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 5 cut(s) 75, 91, 374, 462, 610
MnlI CCTC 6 cut(s) 103, 154, 375, 416, 459, 567
MseI TTAA 2 cut(s) 171, 599
MspCI CTTAAG 1 cut(s) 598
MspI CCGG 3 cut(s) 238, 293, 453
MspR9I CCNGG 4 cut(s) 194, 239, 323, 453
MvaI CCWGG 2 cut(s) 194, 323
MvnI CGCG 1 cut(s) 183
NciI CCSGG 2 cut(s) 239, 453
NdeII GATC 4 cut(s) 3, 265, 298, 310
NlaIII CATG 2 cut(s) 234, 439
NlaIV GGNNCC 3 cut(s) 165, 267, 291
NmuCI GTSAC 2 cut(s) 86, 100
NspI RCATGY 2 cut(s) 234, 439
NspV TTCGAA 1 cut(s) 619
PfeI GAWTC 2 cut(s) 11, 68
PfoI TCCNGGA 2 cut(s) 192, 237
PkrI GCNGC 1 cut(s) 18
Psp6I CCWGG 2 cut(s) 192, 321
PspEI GGTNACC 1 cut(s) 100
PspGI CCWGG 2 cut(s) 192, 321
PspN4I GGNNCC 3 cut(s) 165, 267, 291
PsuI RGATCY 2 cut(s) 265, 298
RsaI GTAC 3 cut(s) 35, 210, 447
RsaNI GTAC 3 cut(s) 34, 209, 446
SaqAI TTAA 2 cut(s) 171, 599
SatI GCNGC 1 cut(s) 17
Sau3AI GATC 4 cut(s) 3, 265, 298, 310
ScrFI CCNGG 4 cut(s) 194, 239, 323, 453
SduI GDGCHC 1 cut(s) 498
SfaNI GCATC 2 cut(s) 232, 428
SfuI TTCGAA 1 cut(s) 619
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 5 cut(s) 75, 91, 374, 462, 610
SspI AATATT 1 cut(s) 632
StyD4I CCNGG 4 cut(s) 192, 237, 321, 451
StyI CCWWGG 2 cut(s) 105, 588
TaaI ACNGT 1 cut(s) 86
TaqI TCGA 2 cut(s) 159, 619
TasI AATT 5 cut(s) 75, 91, 374, 462, 610
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 2 cut(s) 11, 68
Tru1I TTAA 2 cut(s) 171, 599
Tru9I TTAA 2 cut(s) 171, 599
TseFI GTSAC 2 cut(s) 86, 100
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 2 cut(s) 86, 100
TspDTI ATGAA 2 cut(s) 419, 642
Vha464I CTTAAG 1 cut(s) 598
VneI GTGCAC 1 cut(s) 494
XapI RAATTY 2 cut(s) 374, 610
XceI RCATGY 2 cut(s) 234, 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.