Rh5BG273700

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018719
Physical Location & Seq
Reverse (-)
34479442 .. 34481041
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG273700.1

Sequence Viewer

Length: 249 bp
ATGGCTGATTGGGAGAATACATCACCAAAGCAAGTTGAAATACCCTTTAAGCCTGCTCGTGTTCTCCTCCAGGACTTCACTGGAGTACCAGCTGTGATTGACCTTGCATGTATCTGGGATGCCATGGAGGAGCATGGAAATGATCCCAACAAGATAAATCCATTGGTTCCTGTAGACCTTGTTGTCGATCATTCGGTTACAGTTGATGTAGCAAGAACTGTAGATGCACTACAGGAAAATATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230
Pfam Domains
Protein Families
Aconitase Aconitase_C

Protein Analysis

82

Amino Acids

9.11

Weight (kDa)

4.24

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aconitase PF00330 10 - 73 4.5e-12 Aconitase family (aconitate hydratase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018719)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26970
malus_domestica MD03G1199800.v1.1
rosa_multiflora Rmu_sc0000103.1_g000062
rosa_roxburghii Rroxscaffold_1G00041170
rosa_samantha Rh5AG270700 Rh5BG273700 Rh5BG273800 Rh5CG306800
rosa_wichuraiana Rw5G025260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 182
AccI GTMKAC 1 cut(s) 174
AclWI GGATC 1 cut(s) 137
AfaI GTAC 1 cut(s) 87
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
AlwI GGATC 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 15
BaeI ACNNNNGTAYC 2 cut(s) 69, 102
BauI CACGAG 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 71
BfmI CTRYAG 3 cut(s) 171, 219, 230
Bme1390I CCNGG 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 2 cut(s) 109, 214
BpmI CTGGAG 2 cut(s) 53, 102
BsaJI CCNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 85
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 1 cut(s) 124
BseNI ACTGG 1 cut(s) 85
BseRI GAGGAG 2 cut(s) 56, 143
Bsp143I GATC 2 cut(s) 142, 187
Bsp19I CCATGG 1 cut(s) 123
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 1 cut(s) 137
BsrI ACTGG 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 2 cut(s) 142, 187
BssSI CACGAG 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 202, 220
BstC8I GCNNGC 1 cut(s) 54
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 2 cut(s) 145, 190
BstMBI GATC 2 cut(s) 142, 187
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 3 cut(s) 171, 219, 230
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 1 cut(s) 124
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 86
CviAII CATG 3 cut(s) 108, 124, 134
CviJI RGCY 3 cut(s) 5, 52, 92
CviKI_1 RGCY 3 cut(s) 5, 52, 92
CviQI GTAC 1 cut(s) 86
DpnI GATC 2 cut(s) 144, 189
DpnII GATC 2 cut(s) 142, 187
DrdI GACNNNNNNGTC 1 cut(s) 182
DseDI GACNNNNNNGTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 3 cut(s) 111, 127, 137
FaiI YATR 4 cut(s) 109, 125, 135, 242
FatI CATG 3 cut(s) 107, 123, 133
FblI GTMKAC 1 cut(s) 174
FokI GGATG 1 cut(s) 131
GsuI CTGGAG 2 cut(s) 53, 102
Hin1II CATG 3 cut(s) 111, 127, 137
HphI GGTGA 1 cut(s) 15
Hpy166II GTNNAC 1 cut(s) 175
Hpy8I GTNNAC 1 cut(s) 175
HpyCH4III ACNGT 2 cut(s) 202, 220
HpyCH4V TGCA 2 cut(s) 107, 227
Hsp92II CATG 3 cut(s) 111, 127, 137
Kzo9I GATC 2 cut(s) 142, 187
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 8 cut(s) 56, 66, 66, 83, 100, 102, 183, 218
LweI GCATC 2 cut(s) 109, 214
MaeIII GTNAC 1 cut(s) 196
MalI GATC 2 cut(s) 144, 189
MboI GATC 2 cut(s) 142, 187
MnlI CCTC 2 cut(s) 77, 121
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 71
MvaI CCWGG 1 cut(s) 71
NcoI CCATGG 1 cut(s) 123
NdeII GATC 2 cut(s) 142, 187
NlaIII CATG 3 cut(s) 111, 127, 137
NlaIV GGNNCC 1 cut(s) 168
NspI RCATGY 1 cut(s) 111
PfoI TCCNGGA 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 1 cut(s) 168
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
RseI CAYNNNNRTG 1 cut(s) 138
SaqAI TTAA 1 cut(s) 48
Sau3AI GATC 2 cut(s) 142, 187
ScrFI CCNGG 1 cut(s) 71
SetI ASST 3 cut(s) 94, 105, 180
SfaNI GCATC 2 cut(s) 109, 214
SfcI CTRYAG 3 cut(s) 171, 219, 230
SmiMI CAYNNNNRTG 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 202, 220
TaqI TCGA 1 cut(s) 186
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TscAI CASTG 1 cut(s) 85
TspRI CASTG 1 cut(s) 85
XceI RCATGY 1 cut(s) 111
XcmI CCANNNNNNNNNTGG 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.