AT4G27350

Protein of unknown function (DUF1223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013222
Physical Location & Seq
Reverse (-)
13690645 .. 13692832
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27350.1

Sequence Viewer

Length: 837 bp
ATGTCGCGCCGTCTTTTCGCCTGTTTCGGCCTCAAGAAATCTTCAACTAAACCTATCTCTGGTGGTAACAATTCCGCCGCCGTAGCCGCAGAAATTCCCGCCGGAGATGGTCCCGTTCTCGTCCAGCTATTCTCGTCTCAGGGATGTAAAACATCGCCCGCGGCGGAGATGTTGATGTCCCGGCTAGGTCGAGGTGATTTCGACGCTCAGATCCGTGGCGAAGATGGTGGATCTGGATCTCCGACGATGGTTTTGGTGTTTCATGTGGATTATTGGGATTACAAGGGGTGGAAAGATCCGTATGGATCGAGCCAATGGACGGTGAGGCAGAAGGCGTATATAGAGGCGTTGAATCTGGATACGATGTTTACTCCGCAGCTTGTGATCCAAGGTCGTTCTCAATTGATCGGAAACGAAGAGGAGACGCTGCTCAAATCTATCTCCGAAGCGCCTAGGTTTCCTTCTCCAGCCTTCCGGGCGACTTTCCAGAGACCAACCTCAGAGACACTGCAAGTATCACTCACCGGAGCTTTGAGAATGAAAGTAGACGGAGACGGAATGGACATAATGGTGGCAATATACGAAACCGGGCTTGTGAACGACTGTGCAAGAGGAGAGAACGAAGGAAGAATGTTGTCGAATGATTACGTTGTAAGAAAGATGGAAAAGATGTGCACTGTGAAAGATGTAGCACCGAAGAAGCCAGTCTCGGGGACTGCTCATTTTACGCTCTGGGATGGATTCAACAGCAGCAAATGTGGAGTCGCCGTCTTTCTTCAGAACTCTTCTCTTCAGATTTTTGGTACACAGAGTTTTAAATTACCTGATGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1223

Protein Analysis

278

Amino Acids

30.38

Weight (kDa)

6.74

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1223 PF06764 40 - 260 1.2e-60 Protein of unknown function (DUF1223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27350 AT5G54240
fragaria_vesca FvH4_3g21110
malus_domestica MD03G1217700.v1.1 MD11G1235500.v1.1
prunus_persica Prupe.4G191500_v2.0.a1
pyrus_communis pycom03g16730 pycom1049g00020
rosa_chinensis RchiOBHm_Chr5g0036121
rosa_laevigata RLG00000033672
rosa_multiflora Rmu_sc0000382.1_g000011
rosa_roxburghii Rroxscaffold_1G00044620
rosa_rugosa Rorug05G0152700
rosa_samantha Rh5AG247200 Rh5BG247600 Rh5CG278500 Rh5DG255900
rosa_wichuraiana Rw5G022670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 546
AccII CGCG 2 cut(s) 7, 161
AciI CCGC 8 cut(s) 75, 78, 87, 99, 159, 161, 164, 374
AclWI GGATC 6 cut(s) 205, 238, 244, 290, 313, 379
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 2 cut(s) 761, 776
AfaI GTAC 1 cut(s) 805
AfiI CCNNNNNNNGG 3 cut(s) 59, 319, 710
AgsI TTSAA 3 cut(s) 45, 352, 745
AluBI AGCT 3 cut(s) 127, 379, 530
AluI AGCT 3 cut(s) 127, 379, 530
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 6 cut(s) 141, 416, 484, 497, 546, 712
Alw44I GTGCAC 1 cut(s) 673
AlwI GGATC 6 cut(s) 205, 238, 244, 290, 313, 379
Ama87I CYCGRG 1 cut(s) 709
AoxI GGCC 1 cut(s) 28
ApaLI GTGCAC 1 cut(s) 673
ApeKI GCWGC 3 cut(s) 376, 427, 750
ApoI RAATTY 1 cut(s) 93
ArsI GACNNNNNNTTYG 4 cut(s) 235, 267, 747, 779
AspA2I CCTAGG 1 cut(s) 452
AspLEI GCGC 2 cut(s) 9, 451
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 3 cut(s) 181, 476, 589
AsuHPI GGTGA 3 cut(s) 206, 334, 514
AvaI CYCGRG 1 cut(s) 709
AvaII GGWCC 1 cut(s) 110
AvrII CCTAGG 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 677
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 3 cut(s) 388, 414, 762
BccI CCATC 5 cut(s) 101, 218, 241, 655, 731
BceAI ACGGC 2 cut(s) 65, 752
BciVI GTATCC 1 cut(s) 352
BcnI CCSGG 3 cut(s) 181, 476, 589
BcoDI GTCTC 6 cut(s) 141, 416, 484, 497, 546, 712
BfaI CTAG 2 cut(s) 185, 453
BfoI RGCGCY 1 cut(s) 452
BfuI GTATCC 1 cut(s) 352
BglI GCCNNNNNGGC 1 cut(s) 476
BisI GCNGC 6 cut(s) 78, 87, 162, 377, 428, 751
BlnI CCTAGG 1 cut(s) 452
BlsI GCNGC 6 cut(s) 79, 88, 163, 378, 429, 752
Bme1390I CCNGG 3 cut(s) 181, 476, 589
Bme18I GGWCC 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 709
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 3 cut(s) 181, 476, 589
BpmI CTGGAG 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 17
BpuMI CCSGG 3 cut(s) 181, 476, 589
BsaBI GATNNNNATC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 484
BsaJI CCNNGG 4 cut(s) 159, 214, 388, 452
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 319, 710
Bse1I ACTGG 1 cut(s) 704
Bse8I GATNNNNATC 1 cut(s) 235
BseDI CCNNGG 4 cut(s) 159, 214, 388, 452
BseGI GGATG 2 cut(s) 149, 742
BseJI GATNNNNATC 1 cut(s) 235
BseLI CCNNNNNNNGG 3 cut(s) 59, 319, 710
BseMII CTCAG 3 cut(s) 152, 221, 513
BseNI ACTGG 1 cut(s) 704
BseRI GAGGAG 2 cut(s) 434, 627
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 3 cut(s) 388, 414, 762
Bsh1236I CGCG 2 cut(s) 7, 161
BshFI GGCC 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 677
BsiHKCI CYCGRG 1 cut(s) 709
BsiSI CCGG 5 cut(s) 102, 181, 475, 525, 588
BslFI GGGAC 3 cut(s) 96, 163, 727
BslI CCNNNNNNNGG 3 cut(s) 59, 319, 710
BsmAI GTCTC 6 cut(s) 141, 416, 484, 497, 546, 712
BsmBI CGTCTC 3 cut(s) 141, 416, 546
BsmFI GGGAC 3 cut(s) 96, 163, 727
BsnI GGCC 1 cut(s) 30
Bso31I GGTCTC 1 cut(s) 484
BsoBI CYCGRG 1 cut(s) 709
Bsp1286I GDGCHC 1 cut(s) 677
Bsp143I GATC 7 cut(s) 210, 230, 236, 295, 305, 384, 405
BspACI CCGC 8 cut(s) 75, 78, 87, 99, 159, 161, 164, 374
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 3 cut(s) 151, 220, 512
BspFNI CGCG 2 cut(s) 7, 161
BspLI GGNNCC 1 cut(s) 112
BspPI GGATC 6 cut(s) 205, 238, 244, 290, 313, 379
BspTNI GGTCTC 1 cut(s) 484
BsrI ACTGG 1 cut(s) 704
BssECI CCNNGG 4 cut(s) 159, 214, 388, 452
BssMI GATC 7 cut(s) 210, 230, 236, 295, 305, 384, 405
BssT1I CCWWGG 2 cut(s) 388, 452
Bst4CI ACNGT 3 cut(s) 322, 605, 679
Bst6I CTCTTC 3 cut(s) 411, 790, 795
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 3 cut(s) 138, 207, 499
BstDSI CCRYGG 2 cut(s) 159, 214
BstF5I GGATG 2 cut(s) 149, 742
BstFNI CGCG 2 cut(s) 7, 161
BstH2I RGCGCY 1 cut(s) 452
BstHHI GCGC 2 cut(s) 9, 451
BstKTI GATC 7 cut(s) 213, 233, 239, 298, 308, 387, 408
BstMAI GTCTC 6 cut(s) 141, 416, 484, 497, 546, 712
BstMBI GATC 7 cut(s) 210, 230, 236, 295, 305, 384, 405
BstMWI GCNNNNNNNGC 3 cut(s) 83, 86, 476
BstSCI CCNGG 3 cut(s) 179, 474, 587
BstSLI GKGCMC 1 cut(s) 677
BstUI CGCG 2 cut(s) 7, 161
BstV1I GCAGC 3 cut(s) 388, 414, 762
BstX2I RGATCY 4 cut(s) 210, 230, 236, 295
BstYI RGATCY 4 cut(s) 210, 230, 236, 295
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 1 cut(s) 30
BtgI CCRYGG 2 cut(s) 159, 214
BtgZI GCGATG 1 cut(s) 138
BtsCI GGATG 2 cut(s) 149, 742
BtsI GCAGTG 1 cut(s) 506
BtsIMutI CAGTG 2 cut(s) 506, 675
Cac8I GCNNGC 1 cut(s) 159
CfoI GCGC 2 cut(s) 9, 451
Cfr13I GGNCC 1 cut(s) 110
Cfr42I CCGCGG 1 cut(s) 162
CseI GACGC 2 cut(s) 212, 433
Csp6I GTAC 1 cut(s) 804
CviAII CATG 1 cut(s) 263
CviQI GTAC 1 cut(s) 804
DdeI CTNAG 3 cut(s) 138, 207, 499
DpnI GATC 7 cut(s) 212, 232, 238, 297, 307, 386, 407
DpnII GATC 7 cut(s) 210, 230, 236, 295, 305, 384, 405
DraI TTTAAA 1 cut(s) 817
Eam1104I CTCTTC 3 cut(s) 411, 790, 795
EarI CTCTTC 3 cut(s) 411, 790, 795
EciI GGCGGA 2 cut(s) 64, 179
Eco130I CCWWGG 2 cut(s) 388, 452
Eco31I GGTCTC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 110
Eco57I CTGAAG 2 cut(s) 761, 776
Eco88I CYCGRG 1 cut(s) 709
EcoT14I CCWWGG 2 cut(s) 388, 452
ErhI CCWWGG 2 cut(s) 388, 452
Esp3I CGTCTC 3 cut(s) 141, 416, 546
FaeI CATG 1 cut(s) 266
FaiI YATR 6 cut(s) 264, 303, 339, 341, 566, 580
FaqI GGGAC 3 cut(s) 96, 163, 727
FatI CATG 1 cut(s) 262
FauI CCCGC 2 cut(s) 106, 166
FblI GTMKAC 1 cut(s) 546
Fnu4HI GCNGC 6 cut(s) 78, 87, 162, 377, 428, 751
FokI GGATG 2 cut(s) 156, 749
Fsp4HI GCNGC 6 cut(s) 78, 87, 162, 377, 428, 751
FspBI CTAG 2 cut(s) 185, 453
GlaI GCGC 2 cut(s) 8, 450
GluI GCNGC 6 cut(s) 78, 87, 162, 377, 428, 751
GsuI CTGGAG 1 cut(s) 450
HaeII RGCGCY 1 cut(s) 452
HaeIII GGCC 1 cut(s) 30
HapII CCGG 5 cut(s) 102, 181, 475, 525, 588
HgaI GACGC 2 cut(s) 212, 433
HhaI GCGC 2 cut(s) 9, 451
Hin1II CATG 1 cut(s) 266
Hin6I GCGC 2 cut(s) 7, 449
HinP1I GCGC 2 cut(s) 7, 449
HinfI GANTC 3 cut(s) 352, 741, 762
HpaII CCGG 5 cut(s) 102, 181, 475, 525, 588
HphI GGTGA 3 cut(s) 206, 334, 514
Hpy166II GTNNAC 5 cut(s) 369, 547, 598, 675, 806
Hpy188I TCNGA 7 cut(s) 210, 243, 410, 445, 502, 780, 795
Hpy188III TCNNGA 4 cut(s) 34, 234, 356, 487
Hpy8I GTNNAC 5 cut(s) 369, 547, 598, 675, 806
Hpy99I CGWCG 2 cut(s) 206, 247
HpyAV CCTTC 4 cut(s) 325, 471, 481, 617
HpyCH4III ACNGT 3 cut(s) 322, 605, 679
HpyCH4IV ACGT 1 cut(s) 648
HpyCH4V TGCA 3 cut(s) 511, 608, 675
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 86, 476
HpyF3I CTNAG 3 cut(s) 138, 207, 499
HpySE526I ACGT 1 cut(s) 648
Hsp92II CATG 1 cut(s) 266
HspAI GCGC 2 cut(s) 7, 449
KspI CCGCGG 1 cut(s) 162
Kzo9I GATC 7 cut(s) 210, 230, 236, 295, 305, 384, 405
LmnI GCTCC 1 cut(s) 527
Lsp1109I GCAGC 3 cut(s) 388, 414, 762
MaeI CTAG 2 cut(s) 185, 453
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 1 cut(s) 65
MalI GATC 7 cut(s) 212, 232, 238, 297, 307, 386, 407
MboI GATC 7 cut(s) 210, 230, 236, 295, 305, 384, 405
MboII GAAGA 8 cut(s) 33, 233, 428, 639, 709, 767, 777, 782
MfeI CAATTG 1 cut(s) 401
MflI RGATCY 4 cut(s) 210, 230, 236, 295
MhlI GDGCHC 1 cut(s) 677
MluCI AATT 4 cut(s) 70, 93, 401, 818
MlyI GAGTC 1 cut(s) 771
MmeI TCCRAC 1 cut(s) 266
MnlI CCTC 7 cut(s) 41, 185, 318, 337, 412, 508, 605
MseI TTAA 1 cut(s) 816
MslI CAYNNNNRTG 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 5 cut(s) 102, 181, 475, 525, 588
MspR9I CCNGG 3 cut(s) 181, 476, 589
MunI CAATTG 1 cut(s) 401
MvnI CGCG 2 cut(s) 7, 161
MwoI GCNNNNNNNGC 3 cut(s) 83, 86, 476
NciI CCSGG 3 cut(s) 181, 476, 589
NdeII GATC 7 cut(s) 210, 230, 236, 295, 305, 384, 405
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 1 cut(s) 112
PfeI GAWTC 2 cut(s) 352, 741
PkrI GCNGC 6 cut(s) 79, 88, 163, 378, 429, 752
PleI GAGTC 1 cut(s) 770
PpsI GAGTC 1 cut(s) 770
PspN4I GGNNCC 1 cut(s) 112
PspPI GGNCC 1 cut(s) 110
PsuI RGATCY 4 cut(s) 210, 230, 236, 295
RsaI GTAC 1 cut(s) 805
RsaNI GTAC 1 cut(s) 804
RseI CAYNNNNRTG 1 cut(s) 569
SacII CCGCGG 1 cut(s) 162
SaqAI TTAA 1 cut(s) 816
SatI GCNGC 6 cut(s) 78, 87, 162, 377, 428, 751
Sau3AI GATC 7 cut(s) 210, 230, 236, 295, 305, 384, 405
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 1 cut(s) 771
ScrFI CCNGG 3 cut(s) 181, 476, 589
SduI GDGCHC 1 cut(s) 677
Sfr303I CCGCGG 1 cut(s) 162
SgrBI CCGCGG 1 cut(s) 162
SinI GGWCC 1 cut(s) 110
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 4 cut(s) 70, 93, 401, 818
SsiI CCGC 8 cut(s) 75, 78, 87, 99, 159, 161, 164, 374
SspMI CTAG 2 cut(s) 185, 453
StyD4I CCNGG 3 cut(s) 179, 474, 587
StyI CCWWGG 2 cut(s) 388, 452
TaaI ACNGT 3 cut(s) 322, 605, 679
TaiI ACGT 1 cut(s) 651
TaqI TCGA 4 cut(s) 190, 201, 308, 638
TasI AATT 4 cut(s) 70, 93, 401, 818
TauI GCSGC 3 cut(s) 80, 89, 164
TfiI GAWTC 2 cut(s) 352, 741
Tru1I TTAA 1 cut(s) 816
Tru9I TTAA 1 cut(s) 816
TscAI CASTG 2 cut(s) 513, 682
TseI GCWGC 3 cut(s) 376, 427, 750
TspDTI ATGAA 2 cut(s) 251, 554
TspGWI ACGGA 4 cut(s) 203, 288, 564, 570
TspRI CASTG 2 cut(s) 513, 682
VneI GTGCAC 1 cut(s) 673
VpaK11BI GGWCC 1 cut(s) 110
XapI RAATTY 1 cut(s) 93
XmaJI CCTAGG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 546
XspI CTAG 2 cut(s) 185, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.