pycom1049g00020

Protein of unknown function (DUF1223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00001049
OG0013222
Physical Location & Seq
Forward (+)
13938 .. 15950
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom1049g00020.1

Sequence Viewer

Length: 825 bp
ATGGCACGTGGTCTCCTCTCCTGCTTTGGATCAAAAGGGGGCTCGGGCACGTCCCACGAAACGCACGCGGCCGGGGCGACCGCGCATGTTTCGGCAGAGGAGCAGCAGCGGAGCGGACCGGTTCTGGTGGAGCTGTTCTCGTCGCAGGGCTGCACCACCTCGCCTGTCGCGGAGCTGCTGGTGTCGAGGCTTGGGAGGGGGGATTTTGATGGGACGGACCTGCCTCCTGTGGTTGCGCTGGCCTTCCACGTGGACTACTGGGACTACATGGGCTGGAAGGACCCTTATGGGTCCAGCCAGTGGACTGTACGGCAGAAGGCCTATGTTGAGGCCTTGGGTCTGGACACCATGTTCACCCCCCAGATTGTGCTTCAAGGTAGGGCCCAATGTGTTGGCAACGATGAGAGTGCTTTGATAACGTCTATCAAGGATGCACCTAGATATCCTGCACCGGCGTTCCAGGAACAGGCAACATTCCAAAGGCCATCACAGGCATCCTTGCAAGTATCCCTAACAGGATCATTGAGGTCGAAGGTGGACAACTACGGTGCCAATGTGATGGTGGCGCTGTACGAGAACGGCCTGATTACCGACTGCCCCAAGGGAGAGAACCAAGGACGTGTCCTGTCCAATGACTTCGTCGTCCGACGACTTGAAAAGCTCTGCACTGTCAAAGACATTGCTGCAAAGAAGACCATTTCTGGAACCATAAACTTCTCCTTGTGGGACGGTTACAATCCAGCCAAATGTGGCATGGCCCTCTTTGTTCAGAACCCTTCCCATCAAATTTTTGGTTTGCAGGACTTTCAGCTGCCGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1223

Protein Analysis

275

Amino Acids

29.72

Weight (kDa)

5.74

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1223 PF06764 42 - 257 2.2e-62 Protein of unknown function (DUF1223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27350 AT5G54240
fragaria_vesca FvH4_3g21110
malus_domestica MD03G1217700.v1.1 MD11G1235500.v1.1
prunus_persica Prupe.4G191500_v2.0.a1
pyrus_communis pycom03g16730 pycom1049g00020
rosa_chinensis RchiOBHm_Chr5g0036121
rosa_laevigata RLG00000033672
rosa_multiflora Rmu_sc0000382.1_g000011
rosa_roxburghii Rroxscaffold_1G00044620
rosa_rugosa Rorug05G0152700
rosa_samantha Rh5AG247200 Rh5BG247600 Rh5CG278500 Rh5DG255900
rosa_wichuraiana Rw5G022670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
Acc36I ACCTGC 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 548
AccB7I CCANNNNNTGG 1 cut(s) 300
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 3 cut(s) 68, 83, 170
AciI CCGC 5 cut(s) 68, 81, 109, 114, 170
AclWI GGATC 2 cut(s) 37, 526
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 1 cut(s) 786
AcvI CACGTG 2 cut(s) 8, 250
AfaI GTAC 2 cut(s) 309, 572
AfiI CCNNNNNNNGG 2 cut(s) 300, 466
AflIII ACRYGT 1 cut(s) 619
AgeI ACCGGT 1 cut(s) 118
AgsI TTSAA 2 cut(s) 374, 656
AjiI CACGTC 2 cut(s) 51, 620
AjnI CCWGG 1 cut(s) 459
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 4 cut(s) 133, 175, 661, 811
AluI AGCT 4 cut(s) 133, 175, 661, 811
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 2 cut(s) 37, 526
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 8 cut(s) 69, 240, 318, 330, 381, 482, 580, 756
ApaI GGGCCC 1 cut(s) 385
ApeKI GCWGC 6 cut(s) 103, 106, 150, 175, 683, 811
ApoI RAATTY 1 cut(s) 786
AsiGI ACCGGT 1 cut(s) 118
AspLEI GCGC 3 cut(s) 85, 238, 568
AspS9I GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 757
AsuC2I CCSGG 1 cut(s) 73
AsuHPI GGTGA 1 cut(s) 346
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 4 cut(s) 116, 217, 280, 291
BaeGI GKGCMC 2 cut(s) 50, 385
BanI GGYRCC 1 cut(s) 548
BanII GRGCYC 2 cut(s) 44, 385
BbrPI CACGTG 2 cut(s) 8, 250
BbsI GAAGAC 1 cut(s) 698
BbvI GCAGC 6 cut(s) 115, 118, 137, 162, 670, 798
BccI CCATC 4 cut(s) 203, 493, 553, 789
BceAI ACGGC 2 cut(s) 326, 595
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 461
BciVI GTATCC 1 cut(s) 517
BcnI CCSGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 569
BfuAI ACCTGC 1 cut(s) 228
BfuI GTATCC 1 cut(s) 517
BisI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 684, 812
BlsI GCNGC 7 cut(s) 70, 105, 108, 152, 177, 685, 813
Bme1390I CCNGG 2 cut(s) 73, 461
Bme18I GGWCC 4 cut(s) 116, 217, 280, 291
BmeT110I CYCGRG 1 cut(s) 43
BmgBI CACGTC 2 cut(s) 51, 620
BmgT120I GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 757
BmiI GGNNCC 5 cut(s) 282, 292, 383, 550, 706
BmrFI CCNGG 2 cut(s) 73, 461
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 2 cut(s) 421, 503
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 698
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpuMI CCSGG 1 cut(s) 73
BsaAI YACGTR 2 cut(s) 8, 250
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 72, 333, 600, 613
BsaWI WCCGGW 1 cut(s) 118
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 300, 466
Bse118I RCCGGY 2 cut(s) 118, 451
Bse1I ACTGG 2 cut(s) 263, 298
Bse3DI GCAATG 1 cut(s) 678
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 4 cut(s) 72, 333, 600, 613
BseGI GGATG 2 cut(s) 436, 494
BseLI CCNNNNNNNGG 2 cut(s) 300, 466
BseMI GCAATG 1 cut(s) 678
BseNI ACTGG 2 cut(s) 263, 298
BseRI GAGGAG 2 cut(s) 5, 113
BseSI GKGCMC 2 cut(s) 50, 385
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 6 cut(s) 115, 118, 137, 162, 670, 798
BsgI GTGCAG 3 cut(s) 136, 432, 649
Bsh1236I CGCG 3 cut(s) 68, 83, 170
Bsh1285I CGRYCG 2 cut(s) 72, 81
BshFI GGCC 8 cut(s) 71, 242, 320, 332, 383, 484, 582, 758
BshNI GGYRCC 1 cut(s) 548
BshTI ACCGGT 1 cut(s) 118
BsiEI CGRYCG 2 cut(s) 72, 81
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 4 cut(s) 72, 119, 452, 815
BslFI GGGAC 4 cut(s) 37, 226, 275, 740
BslI CCNNNNNNNGG 2 cut(s) 300, 466
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 4 cut(s) 37, 226, 275, 740
BsnI GGCC 8 cut(s) 71, 242, 320, 332, 383, 484, 582, 758
Bso31I GGTCTC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 43
Bsp120I GGGCCC 1 cut(s) 381
Bsp1286I GDGCHC 3 cut(s) 44, 50, 385
Bsp143I GATC 2 cut(s) 29, 518
BspACI CCGC 5 cut(s) 68, 81, 109, 114, 170
BspANI GGCC 8 cut(s) 71, 242, 320, 332, 383, 484, 582, 758
BspFNI CGCG 3 cut(s) 68, 83, 170
BspLI GGNNCC 5 cut(s) 282, 292, 383, 550, 706
BspMI ACCTGC 1 cut(s) 228
BspPI GGATC 2 cut(s) 37, 526
BspT107I GGYRCC 1 cut(s) 548
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 2 cut(s) 118, 451
BsrI ACTGG 2 cut(s) 263, 298
BssAI RCCGGY 2 cut(s) 118, 451
BssECI CCNNGG 4 cut(s) 72, 333, 600, 613
BssMI GATC 2 cut(s) 29, 518
BssT1I CCWWGG 3 cut(s) 333, 600, 613
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 4 cut(s) 307, 548, 670, 731
BstBAI YACGTR 2 cut(s) 8, 250
BstC8I GCNNGC 2 cut(s) 66, 240
BstF5I GGATG 2 cut(s) 436, 494
BstFNI CGCG 3 cut(s) 68, 83, 170
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 3 cut(s) 85, 238, 568
BstKTI GATC 2 cut(s) 32, 521
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 2 cut(s) 29, 518
BstMCI CGRYCG 2 cut(s) 72, 81
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 1 cut(s) 461
BstNSI RCATGY 1 cut(s) 89
BstSCI CCNGG 2 cut(s) 71, 459
BstSLI GKGCMC 2 cut(s) 50, 385
BstUI CGCG 3 cut(s) 68, 83, 170
BstV1I GCAGC 6 cut(s) 115, 118, 137, 162, 670, 798
BstV2I GAAGAC 1 cut(s) 698
BstXI CCANNNNNNTGG 2 cut(s) 392, 559
BstZI CGGCCG 1 cut(s) 69
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 8 cut(s) 71, 242, 320, 332, 383, 484, 582, 758
BtrI CACGTC 2 cut(s) 51, 620
BtsCI GGATG 2 cut(s) 436, 494
BtsIMutI CAGTG 2 cut(s) 305, 666
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 2 cut(s) 66, 240
CfoI GCGC 3 cut(s) 85, 238, 568
Cfr10I RCCGGY 2 cut(s) 118, 451
Cfr13I GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 757
CpoI CGGWCCG 1 cut(s) 116
Csp6I GTAC 2 cut(s) 308, 571
CspAI ACCGGT 1 cut(s) 118
CspI CGGWCCG 1 cut(s) 116
CviAII CATG 4 cut(s) 86, 268, 349, 754
CviQI GTAC 2 cut(s) 308, 571
DpnI GATC 2 cut(s) 31, 520
DpnII GATC 2 cut(s) 29, 518
DrdI GACNNNNNNGTC 1 cut(s) 641
DseDI GACNNNNNNGTC 1 cut(s) 641
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 333, 600, 613
Eco147I AGGCCT 2 cut(s) 320, 332
Eco24I GRGCYC 2 cut(s) 44, 385
Eco31I GGTCTC 1 cut(s) 17
Eco32I GATATC 1 cut(s) 443
Eco47I GGWCC 4 cut(s) 116, 217, 280, 291
Eco52I CGGCCG 1 cut(s) 69
Eco72I CACGTG 2 cut(s) 8, 250
Eco88I CYCGRG 1 cut(s) 43
EcoO109I RGGNCCY 2 cut(s) 280, 381
EcoRII CCWGG 1 cut(s) 459
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 3 cut(s) 333, 600, 613
EcoT38I GRGCYC 2 cut(s) 44, 385
ErhI CCWWGG 3 cut(s) 333, 600, 613
FaeI CATG 4 cut(s) 89, 271, 352, 757
FaiI YATR 7 cut(s) 87, 269, 288, 324, 350, 710, 755
FaqI GGGAC 4 cut(s) 37, 226, 275, 740
FatI CATG 4 cut(s) 85, 267, 348, 753
Fnu4HI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 684, 812
FokI GGATG 2 cut(s) 443, 481
FriOI GRGCYC 2 cut(s) 44, 385
Fsp4HI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 684, 812
FspBI CTAG 1 cut(s) 438
GlaI GCGC 3 cut(s) 84, 237, 567
GluI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 684, 812
HaeII RGCGCY 1 cut(s) 569
HaeIII GGCC 8 cut(s) 71, 242, 320, 332, 383, 484, 582, 758
HapII CCGG 4 cut(s) 72, 119, 452, 815
HhaI GCGC 3 cut(s) 85, 238, 568
Hin1II CATG 4 cut(s) 89, 271, 352, 757
Hin6I GCGC 3 cut(s) 83, 236, 566
HinP1I GCGC 3 cut(s) 83, 236, 566
HpaII CCGG 4 cut(s) 72, 119, 452, 815
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 4 cut(s) 253, 303, 354, 538
Hpy188I TCNGA 2 cut(s) 647, 771
Hpy188III TCNNGA 2 cut(s) 341, 702
Hpy8I GTNNAC 4 cut(s) 253, 303, 354, 538
Hpy99I CGWCG 3 cut(s) 145, 644, 651
HpyAV CCTTC 5 cut(s) 253, 271, 310, 526, 786
HpyCH4III ACNGT 4 cut(s) 307, 548, 670, 731
HpyCH4IV ACGT 5 cut(s) 7, 50, 249, 419, 619
HpyCH4V TGCA 7 cut(s) 153, 434, 449, 502, 666, 686, 799
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpySE526I ACGT 5 cut(s) 7, 50, 249, 419, 619
Hsp92II CATG 4 cut(s) 89, 271, 352, 757
HspAI GCGC 3 cut(s) 83, 236, 566
Kzo9I GATC 2 cut(s) 29, 518
LmnI GCTCC 4 cut(s) 100, 111, 130, 172
Lsp1109I GCAGC 6 cut(s) 115, 118, 137, 162, 670, 798
LweI GCATC 2 cut(s) 421, 503
MaeI CTAG 1 cut(s) 438
MaeII ACGT 5 cut(s) 7, 50, 249, 419, 619
MaeIII GTNAC 1 cut(s) 731
MalI GATC 2 cut(s) 31, 520
MbiI CCGCTC 1 cut(s) 114
MboI GATC 2 cut(s) 29, 518
MboII GAAGA 1 cut(s) 703
MfeI CAATTG 1 cut(s) 820
MhlI GDGCHC 3 cut(s) 44, 50, 385
MluCI AATT 2 cut(s) 786, 820
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 9 cut(s) 26, 91, 169, 180, 189, 234, 322, 519, 770
MspA1I CMGCKG 2 cut(s) 109, 811
MspI CCGG 4 cut(s) 72, 119, 452, 815
MspR9I CCNGG 2 cut(s) 73, 461
MunI CAATTG 1 cut(s) 820
MvaI CCWGG 1 cut(s) 461
MvnI CGCG 3 cut(s) 68, 83, 170
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 1 cut(s) 73
NdeII GATC 2 cut(s) 29, 518
NlaIII CATG 4 cut(s) 89, 271, 352, 757
NlaIV GGNNCC 5 cut(s) 282, 292, 383, 550, 706
NspI RCATGY 1 cut(s) 89
PceI AGGCCT 2 cut(s) 320, 332
PflFI GACNNNGTC 1 cut(s) 638
PflMI CCANNNNNTGG 1 cut(s) 300
PfoI TCCNGGA 1 cut(s) 459
PinAI ACCGGT 1 cut(s) 118
PkrI GCNGC 7 cut(s) 70, 105, 108, 152, 177, 685, 813
PmaCI CACGTG 2 cut(s) 8, 250
PmlI CACGTG 2 cut(s) 8, 250
Ppu21I YACGTR 2 cut(s) 8, 250
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 459
PspCI CACGTG 2 cut(s) 8, 250
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 5 cut(s) 282, 292, 383, 550, 706
PspOMI GGGCCC 1 cut(s) 381
PspPI GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 757
PspPPI RGGWCCY 1 cut(s) 280
PsyI GACNNNGTC 1 cut(s) 638
PvuII CAGCTG 1 cut(s) 811
RsaI GTAC 2 cut(s) 309, 572
RsaNI GTAC 2 cut(s) 308, 571
Rsr2I CGGWCCG 1 cut(s) 116
RsrII CGGWCCG 1 cut(s) 116
SatI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 684, 812
Sau3AI GATC 2 cut(s) 29, 518
Sau96I GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 757
ScrFI CCNGG 2 cut(s) 73, 461
SduI GDGCHC 3 cut(s) 44, 50, 385
SfaNI GCATC 2 cut(s) 421, 503
SgrAI CRCCGGYG 1 cut(s) 451
SinI GGWCC 4 cut(s) 116, 217, 280, 291
Sse9I AATT 2 cut(s) 786, 820
SseBI AGGCCT 2 cut(s) 320, 332
SsiI CCGC 5 cut(s) 68, 81, 109, 114, 170
SspMI CTAG 1 cut(s) 438
StuI AGGCCT 2 cut(s) 320, 332
StyD4I CCNGG 2 cut(s) 71, 459
StyI CCWWGG 3 cut(s) 333, 600, 613
TaaI ACNGT 4 cut(s) 307, 548, 670, 731
TaiI ACGT 5 cut(s) 10, 53, 252, 422, 622
TaqI TCGA 2 cut(s) 185, 530
TasI AATT 2 cut(s) 786, 820
TauI GCSGC 1 cut(s) 71
TscAI CASTG 2 cut(s) 305, 673
TseI GCWGC 6 cut(s) 103, 106, 150, 175, 683, 811
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 2 cut(s) 305, 673
Tth111I GACNNNGTC 1 cut(s) 638
Van91I CCANNNNNTGG 1 cut(s) 300
VpaK11BI GGWCC 4 cut(s) 116, 217, 280, 291
XapI RAATTY 1 cut(s) 786
XceI RCATGY 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 3 cut(s) 559, 751, 788
XspI CTAG 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.