Rmu_sc0000382.1_g000011

Protein of unknown function (DUF1223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000382.1
OG0013222
Physical Location & Seq
Reverse (-)
60259 .. 62026
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000382.1_g000011.1.cds

Sequence Viewer

Length: 831 bp
atggggcgtcgtctcttcgcctgctttggaaaaagcctctcctcctccccctcctcctcggccgcgcatgagaaacacacgtcagcggctacagctgacgtgtcggccgaggagcagaggaagagcgggccggtgctggtggagctgttctcgtcgcagggctgcaccacctcgcctgtggcggagctgcttctgtcgaggcttgggagaggggatttcaacgggagtgacgtgcctccggtggtggtgctggcgtaccacgtggactactgggactacatggggtggaaggacccgtacggttcgagccagtggaccgttcggcaaaaggcttatattgaggccttgaagcaggacaccatgttcacgcctcaggtggtggttcaaggtacggctcaatgtgttggcaacgatgagaatgatttgctaacctccatcaagcaggcaaccagatatgctgcacccacattccaggcaactttcgaaagaccatctccagactcgttacaagtatctctaacaggagctttaaggacaaaggtggatagcaatggcgccaatgtgatggtggcgctctatgaaagtgggttagtcactgactgcccctcgggagagaacaaaggacgcgtcctgtccaacgactatgttgttcgaagactcgaaaagctctgcacggttaaggacgtcccagccaagaagaccatttcagggactattaccttcccattatggcaaagtttcaatgccaccaaatgtgggatggtcgtcttcattcaaaacagctcgcatcaaatttttggttctcagaagtttcagctgccggacaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1223

Protein Analysis

276

Amino Acids

30.11

Weight (kDa)

7.59

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27350 AT5G54240
fragaria_vesca FvH4_3g21110
malus_domestica MD03G1217700.v1.1 MD11G1235500.v1.1
prunus_persica Prupe.4G191500_v2.0.a1
pyrus_communis pycom03g16730 pycom1049g00020
rosa_chinensis RchiOBHm_Chr5g0036121
rosa_laevigata RLG00000033672
rosa_multiflora Rmu_sc0000382.1_g000011
rosa_roxburghii Rroxscaffold_1G00044620
rosa_rugosa Rorug05G0152700
rosa_samantha Rh5AG247200 Rh5BG247600 Rh5CG278500 Rh5DG255900
rosa_wichuraiana Rw5G022670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 554
AccBSI CCGCTC 1 cut(s) 126
AccII CGCG 2 cut(s) 65, 627
AciI CCGC 4 cut(s) 63, 86, 126, 182
AcoI YGGCCR 2 cut(s) 60, 105
AcsI RAATTY 1 cut(s) 792
AcvI CACGTG 1 cut(s) 262
AcyI GRCGYC 3 cut(s) 7, 555, 684
AfaI GTAC 3 cut(s) 257, 299, 391
AfiI CCNNNNNNNGG 2 cut(s) 708, 756
AflIII ACRYGT 3 cut(s) 78, 99, 625
AgsI TTSAA 5 cut(s) 220, 349, 386, 742, 776
AjiI CACGTC 3 cut(s) 81, 100, 232
AjnI CCWGG 1 cut(s) 471
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
AluBI AGCT 7 cut(s) 95, 145, 187, 527, 667, 783, 817
AluI AGCT 7 cut(s) 95, 145, 187, 527, 667, 783, 817
Alw26I GTCTC 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 607
AoxI GGCC 4 cut(s) 60, 105, 128, 342
ApeKI GCWGC 4 cut(s) 162, 187, 458, 817
ApoI RAATTY 1 cut(s) 792
ArsI GACNNNNNNTTYG 2 cut(s) 612, 644
AspLEI GCGC 3 cut(s) 67, 557, 574
AspS9I GGNCC 3 cut(s) 128, 292, 315
AsuII TTCGAA 2 cut(s) 483, 652
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 2 cut(s) 292, 315
AxyI CCTNAGG 1 cut(s) 372
BanI GGYRCC 1 cut(s) 554
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
BbrPI CACGTG 1 cut(s) 262
BbsI GAAGAC 3 cut(s) 661, 704, 760
BbvI GCAGC 4 cut(s) 149, 174, 445, 804
BccI CCATC 4 cut(s) 443, 499, 559, 754
BceAI ACGGC 1 cut(s) 408
BciT130I CCWGG 1 cut(s) 473
BcoDI GTCTC 1 cut(s) 17
BfmI CTRYAG 1 cut(s) 90
BfoI RGCGCY 2 cut(s) 558, 575
BisI GCNGC 6 cut(s) 63, 87, 163, 188, 459, 818
BlsI GCNGC 6 cut(s) 64, 88, 164, 189, 460, 819
Bme1390I CCNGG 1 cut(s) 473
Bme18I GGWCC 2 cut(s) 292, 315
BmeT110I CYCGRG 1 cut(s) 607
BmgBI CACGTC 3 cut(s) 81, 100, 232
BmgT120I GGNCC 3 cut(s) 128, 292, 315
BmiI GGNNCC 2 cut(s) 294, 556
BmrFI CCNGG 1 cut(s) 473
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 796
BmuI ACTGGG 1 cut(s) 280
BpiI GAAGAC 3 cut(s) 661, 704, 760
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 480
Bpu14I TTCGAA 2 cut(s) 483, 652
BsaAI YACGTR 1 cut(s) 262
BsaHI GRCGYC 3 cut(s) 7, 555, 684
BsaJI CCNNGG 3 cut(s) 57, 108, 606
BsaWI WCCGGW 1 cut(s) 238
Bsc4I CCNNNNNNNGG 2 cut(s) 708, 756
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 275, 310
Bse21I CCTNAGG 1 cut(s) 372
Bse3DI GCAATG 1 cut(s) 556
BseBI CCWGG 1 cut(s) 473
BseDI CCNNGG 3 cut(s) 57, 108, 606
BseGI GGATG 1 cut(s) 765
BseLI CCNNNNNNNGG 2 cut(s) 708, 756
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 2 cut(s) 386, 818
BseNI ACTGG 2 cut(s) 275, 310
BseRI GAGGAG 5 cut(s) 31, 34, 43, 46, 125
BseX3I CGGCCG 2 cut(s) 60, 105
BseXI GCAGC 4 cut(s) 149, 174, 445, 804
BseYI CCCAGC 1 cut(s) 688
BsgI GTGCAG 3 cut(s) 148, 444, 655
Bsh1236I CGCG 2 cut(s) 65, 627
Bsh1285I CGRYCG 2 cut(s) 63, 108
BshFI GGCC 4 cut(s) 62, 107, 130, 344
BshNI GGYRCC 1 cut(s) 554
BsiEI CGRYCG 2 cut(s) 63, 108
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 3 cut(s) 131, 239, 821
BsiWI CGTACG 1 cut(s) 297
BslFI GGGAC 3 cut(s) 287, 671, 724
BslI CCNNNNNNNGG 2 cut(s) 708, 756
BsmAI GTCTC 1 cut(s) 17
BsmBI CGTCTC 1 cut(s) 17
BsmFI GGGAC 3 cut(s) 287, 671, 724
BsnI GGCC 4 cut(s) 62, 107, 130, 344
BsoBI CYCGRG 1 cut(s) 607
Bsp119I TTCGAA 2 cut(s) 483, 652
BspACI CCGC 4 cut(s) 63, 86, 126, 182
BspANI GGCC 4 cut(s) 62, 107, 130, 344
BspCNI CTCAG 2 cut(s) 385, 817
BspFNI CGCG 2 cut(s) 65, 627
BspLI GGNNCC 2 cut(s) 294, 556
BspQI GCTCTTC 1 cut(s) 116
BspT104I TTCGAA 2 cut(s) 483, 652
BspT107I GGYRCC 1 cut(s) 554
BsrBI CCGCTC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 556
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 275, 310
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 57, 108, 606
BssNI GRCGYC 3 cut(s) 7, 555, 684
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 3 cut(s) 302, 319, 676
Bst6I CTCTTC 2 cut(s) 20, 116
BstACI GRCGYC 3 cut(s) 7, 555, 684
BstBAI YACGTR 1 cut(s) 262
BstBI TTCGAA 2 cut(s) 483, 652
BstC8I GCNNGC 5 cut(s) 22, 128, 252, 444, 785
BstDEI CTNAG 2 cut(s) 372, 804
BstF5I GGATG 1 cut(s) 765
BstFNI CGCG 2 cut(s) 65, 627
BstH2I RGCGCY 2 cut(s) 558, 575
BstHHI GCGC 3 cut(s) 67, 557, 574
BstMAI GTCTC 1 cut(s) 17
BstMCI CGRYCG 2 cut(s) 63, 108
BstMWI GCNNNNNNNGC 2 cut(s) 92, 142
BstNI CCWGG 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 471
BstSFI CTRYAG 1 cut(s) 90
BstUI CGCG 2 cut(s) 65, 627
BstV1I GCAGC 4 cut(s) 149, 174, 445, 804
BstV2I GAAGAC 3 cut(s) 661, 704, 760
BstXI CCANNNNNNTGG 1 cut(s) 565
BstZI CGGCCG 2 cut(s) 60, 105
Bsu36I CCTNAGG 1 cut(s) 372
BsuRI GGCC 4 cut(s) 62, 107, 130, 344
BtrI CACGTC 3 cut(s) 81, 100, 232
BtsCI GGATG 1 cut(s) 765
BtsIMutI CAGTG 2 cut(s) 317, 594
Cac8I GCNNGC 5 cut(s) 22, 128, 252, 444, 785
CfoI GCGC 3 cut(s) 67, 557, 574
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 3 cut(s) 128, 292, 315
CseI GACGC 2 cut(s) 616, 633
Csp6I GTAC 3 cut(s) 256, 298, 390
CviAII CATG 3 cut(s) 68, 280, 361
CviQI GTAC 3 cut(s) 256, 298, 390
DdeI CTNAG 2 cut(s) 372, 804
DinI GGCGCC 1 cut(s) 556
EaeI YGGCCR 2 cut(s) 60, 105
EagI CGGCCG 2 cut(s) 60, 105
Eam1104I CTCTTC 2 cut(s) 20, 116
EarI CTCTTC 2 cut(s) 20, 116
EciI GGCGGA 1 cut(s) 197
EclXI CGGCCG 2 cut(s) 60, 105
Eco147I AGGCCT 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 292, 315
Eco52I CGGCCG 2 cut(s) 60, 105
Eco72I CACGTG 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 607
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 471
EgeI GGCGCC 1 cut(s) 556
EheI GGCGCC 1 cut(s) 556
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 3 cut(s) 71, 283, 364
FaiI YATR 8 cut(s) 69, 281, 336, 362, 456, 579, 645, 730
FaqI GGGAC 3 cut(s) 287, 671, 724
FatI CATG 3 cut(s) 67, 279, 360
FauI CCCGC 1 cut(s) 119
Fnu4HI GCNGC 6 cut(s) 63, 87, 163, 188, 459, 818
FokI GGATG 1 cut(s) 772
Fsp4HI GCNGC 6 cut(s) 63, 87, 163, 188, 459, 818
GlaI GCGC 3 cut(s) 66, 556, 573
GluI GCNGC 6 cut(s) 63, 87, 163, 188, 459, 818
GsaI CCCAGC 1 cut(s) 692
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 2 cut(s) 558, 575
HaeIII GGCC 4 cut(s) 62, 107, 130, 344
HapII CCGG 3 cut(s) 131, 239, 821
HgaI GACGC 2 cut(s) 616, 633
HhaI GCGC 3 cut(s) 67, 557, 574
Hin1I GRCGYC 3 cut(s) 7, 555, 684
Hin1II CATG 3 cut(s) 71, 283, 364
Hin6I GCGC 3 cut(s) 65, 555, 572
HinP1I GCGC 3 cut(s) 65, 555, 572
HinfI GANTC 2 cut(s) 500, 657
HpaII CCGG 3 cut(s) 131, 239, 821
Hpy166II GTNNAC 3 cut(s) 265, 315, 366
Hpy188I TCNGA 1 cut(s) 807
Hpy188III TCNNGA 2 cut(s) 497, 609
Hpy8I GTNNAC 3 cut(s) 265, 315, 366
Hpy99I CGWCG 2 cut(s) 12, 157
HpyAV CCTTC 2 cut(s) 283, 730
HpyCH4III ACNGT 3 cut(s) 302, 319, 676
HpyCH4IV ACGT 5 cut(s) 80, 99, 231, 261, 684
HpyCH4V TGCA 3 cut(s) 165, 461, 672
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 142
HpyF3I CTNAG 2 cut(s) 372, 804
HpySE526I ACGT 5 cut(s) 80, 99, 231, 261, 684
Hsp92I GRCGYC 3 cut(s) 7, 555, 684
Hsp92II CATG 3 cut(s) 71, 283, 364
HspAI GCGC 3 cut(s) 65, 555, 572
KasI GGCGCC 1 cut(s) 554
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 4 cut(s) 112, 142, 184, 524
Lsp1109I GCAGC 4 cut(s) 149, 174, 445, 804
LweI GCATC 1 cut(s) 796
MaeII ACGT 5 cut(s) 80, 99, 231, 261, 684
MaeIII GTNAC 3 cut(s) 227, 504, 592
MbiI CCGCTC 1 cut(s) 126
MboII GAAGA 5 cut(s) 7, 133, 666, 709, 760
MfeI CAATTG 1 cut(s) 826
MluCI AATT 2 cut(s) 792, 826
MluI ACGCGT 1 cut(s) 625
Mly113I GGCGCC 1 cut(s) 555
MlyI GAGTC 2 cut(s) 494, 651
MmeI TCCRAC 1 cut(s) 660
MseI TTAA 2 cut(s) 530, 678
MspA1I CMGCKG 3 cut(s) 86, 95, 817
MspI CCGG 3 cut(s) 131, 239, 821
MspR9I CCNGG 1 cut(s) 473
MunI CAATTG 1 cut(s) 826
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 2 cut(s) 65, 627
MwoI GCNNNNNNNGC 2 cut(s) 92, 142
NarI GGCGCC 1 cut(s) 555
NlaIII CATG 3 cut(s) 71, 283, 364
NlaIV GGNNCC 2 cut(s) 294, 556
NmeAIII GCCGAG 2 cut(s) 38, 133
NmuCI GTSAC 2 cut(s) 227, 592
NspV TTCGAA 2 cut(s) 483, 652
PceI AGGCCT 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 228
Pfl23II CGTACG 1 cut(s) 297
PkrI GCNGC 6 cut(s) 64, 88, 164, 189, 460, 819
PleI GAGTC 2 cut(s) 494, 651
PluTI GGCGCC 1 cut(s) 558
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 2 cut(s) 494, 651
Ppu21I YACGTR 1 cut(s) 262
PpuMI RGGWCCY 1 cut(s) 292
Psp5II RGGWCCY 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 471
PspCI CACGTG 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 688
PspGI CCWGG 1 cut(s) 471
PspLI CGTACG 1 cut(s) 297
PspN4I GGNNCC 2 cut(s) 294, 556
PspPI GGNCC 3 cut(s) 128, 292, 315
PspPPI RGGWCCY 1 cut(s) 292
PvuII CAGCTG 2 cut(s) 95, 817
RsaI GTAC 3 cut(s) 257, 299, 391
RsaNI GTAC 3 cut(s) 256, 298, 390
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 2 cut(s) 530, 678
SatI GCNGC 6 cut(s) 63, 87, 163, 188, 459, 818
Sau96I GGNCC 3 cut(s) 128, 292, 315
SchI GAGTC 2 cut(s) 494, 651
ScrFI CCNGG 1 cut(s) 473
SfaNI GCATC 1 cut(s) 796
SfcI CTRYAG 1 cut(s) 90
SfoI GGCGCC 1 cut(s) 556
SfuI TTCGAA 2 cut(s) 483, 652
SinI GGWCC 2 cut(s) 292, 315
Sse9I AATT 2 cut(s) 792, 826
SseBI AGGCCT 1 cut(s) 344
SsiI CCGC 4 cut(s) 63, 86, 126, 182
SspDI GGCGCC 1 cut(s) 554
StuI AGGCCT 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 471
TaaI ACNGT 3 cut(s) 302, 319, 676
TaiI ACGT 5 cut(s) 83, 102, 234, 264, 687
TaqI TCGA 5 cut(s) 197, 305, 483, 652, 660
TasI AATT 2 cut(s) 792, 826
TauI GCSGC 2 cut(s) 65, 89
Tru1I TTAA 2 cut(s) 530, 678
Tru9I TTAA 2 cut(s) 530, 678
TscAI CASTG 2 cut(s) 317, 601
TseFI GTSAC 2 cut(s) 227, 592
TseI GCWGC 4 cut(s) 162, 187, 458, 817
Tsp45I GTSAC 2 cut(s) 227, 592
TspDTI ATGAA 2 cut(s) 594, 760
TspRI CASTG 2 cut(s) 317, 601
VpaK11BI GGWCC 2 cut(s) 292, 315
XapI RAATTY 1 cut(s) 792
XcmI CCANNNNNNNNNTGG 3 cut(s) 175, 565, 757
ZraI GACGTC 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.