FvH4_3g21110

Protein of unknown function (DUF1223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013222
Physical Location & Seq
Forward (+)
14141966 .. 14144099
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21110.t1

Sequence Viewer

Length: 834 bp
ATGGGGCGTGGTCTCTTCGCCTGCTTTGGAAAAGGCCACTCCTCTTCTTCCCCCGCCTCGCGTGAGAAACGCACGTCAGCCGTAGCCTCTACCGCTGACGTGTCGGCCGAGGAGCAGAGGAAGAGCGGGCCGGTGTTAGTGGAGCTGTTCTCGTCGCAGGGCTGCGCCACATCGCCGGTGGCGGAGCTGCTCTGTTCGAGGCTTGGGAGAGGGGATTTCAACGGGAGTGACGTGCCTCCGGTGGTGGTGCTCGCGTACCACGTCGATTACTGGGACTACATGGGGTGGAAGGACCCCTATGGTTCGAGCCAGTGGACGGTTCGGCAAAAGAATTACATTGAGGCCTTGAGGCTGGACACCATGTTCACGCCTCAGGTGGTGGTTCAGGGTTCGGTTCATTGTGTTGGCAACGATGAAAACGATTTGCTAACCTGCATCAAACAGGCACCTAGATACGCTGGTCCCACGTTCCAGGCAACTTTCGAAAGATCGGCTTCTTCGGACTCGTTGCAAGTGTCTCTAACAGGAGCTTTAAGGACAAAGGTGGATGGCAATGGCGTCAATGTGATGGTGGCGCTCTATGAAAGTGGGTTAGTCACTGACTGTCCCTCGGGAGAGAACAAGGGACGCGTTCTGTCCAACGACTATGTTGTAAGAAGACTCGAAAAGCTTTGCACAGTTAAGGACATCACAGCCAAGAAGACCATTTCAGGGACTGTTACCTTCCCATTATGGGAAAGTTTCAATGCCACAAAATGTGGGATGGTCGTCTTCATTCAGAGCAGCTCGCATCAAATCTTTAGTTCGCAGAAGTTTCAGCTGCCGGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1223

Protein Analysis

278

Amino Acids

30.18

Weight (kDa)

7.54

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1223 PF06764 46 - 260 5.7e-63 Protein of unknown function (DUF1223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27350 AT5G54240
fragaria_vesca FvH4_3g21110
malus_domestica MD03G1217700.v1.1 MD11G1235500.v1.1
prunus_persica Prupe.4G191500_v2.0.a1
pyrus_communis pycom03g16730 pycom1049g00020
rosa_chinensis RchiOBHm_Chr5g0036121
rosa_laevigata RLG00000033672
rosa_multiflora Rmu_sc0000382.1_g000011
rosa_roxburghii Rroxscaffold_1G00044620
rosa_rugosa Rorug05G0152700
rosa_samantha Rh5AG247200 Rh5BG247600 Rh5CG278500 Rh5DG255900
rosa_wichuraiana Rw5G022670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 440
AccB1I GGYRCC 1 cut(s) 445
AccBSI CCGCTC 1 cut(s) 126
AccII CGCG 3 cut(s) 61, 254, 630
AciI CCGC 4 cut(s) 54, 93, 126, 182
AcoI YGGCCR 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 316, 711, 733
AflIII ACRYGT 2 cut(s) 99, 628
AgsI TTSAA 2 cut(s) 220, 745
AjiI CACGTC 4 cut(s) 75, 100, 232, 262
AjnI CCWGG 1 cut(s) 471
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
AluBI AGCT 6 cut(s) 145, 187, 530, 670, 786, 820
AluI AGCT 6 cut(s) 145, 187, 530, 670, 786, 820
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 17, 522
AlwNI CAGNNNCTG 1 cut(s) 716
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 4 cut(s) 34, 105, 128, 342
ApeKI GCWGC 4 cut(s) 162, 187, 783, 820
AspLEI GCGC 2 cut(s) 167, 577
AspS9I GGNCC 3 cut(s) 128, 292, 461
AsuII TTCGAA 1 cut(s) 483
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 2 cut(s) 292, 461
AxyI CCTNAGG 1 cut(s) 372
BaeI ACNNNNGTAYC 2 cut(s) 445, 478
BanI GGYRCC 1 cut(s) 445
BbsI GAAGAC 3 cut(s) 664, 707, 763
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 4 cut(s) 149, 174, 795, 807
BccI CCATC 3 cut(s) 542, 562, 757
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 1 cut(s) 473
BcoDI GTCTC 2 cut(s) 17, 522
BfaI CTAG 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 578
BfuAI ACCTGC 1 cut(s) 440
BisI GCNGC 4 cut(s) 163, 188, 784, 821
BlsI GCNGC 4 cut(s) 164, 189, 785, 822
Bme1390I CCNGG 1 cut(s) 473
Bme18I GGWCC 2 cut(s) 292, 461
BmeT110I CYCGRG 1 cut(s) 610
BmgBI CACGTC 4 cut(s) 75, 100, 232, 262
BmgT120I GGNCC 3 cut(s) 128, 292, 461
BmiI GGNNCC 3 cut(s) 294, 447, 463
BmrFI CCNGG 1 cut(s) 473
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 2 cut(s) 444, 799
BmuI ACTGGG 1 cut(s) 280
BpiI GAAGAC 3 cut(s) 664, 707, 763
BplI GAGNNNNNCTC 2 cut(s) 134, 166
Bpu14I TTCGAA 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 558
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 108, 609
BsaWI WCCGGW 1 cut(s) 238
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 711, 733
Bse118I RCCGGY 2 cut(s) 130, 175
Bse1I ACTGG 2 cut(s) 275, 310
Bse21I CCTNAGG 1 cut(s) 372
Bse3DI GCAATG 1 cut(s) 559
BseBI CCWGG 1 cut(s) 473
BseDI CCNNGG 2 cut(s) 108, 609
BseGI GGATG 2 cut(s) 553, 768
BseLI CCNNNNNNNGG 3 cut(s) 316, 711, 733
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 2 cut(s) 275, 310
BseRI GAGGAG 2 cut(s) 31, 125
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 4 cut(s) 149, 174, 795, 807
Bsh1236I CGCG 3 cut(s) 61, 254, 630
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 4 cut(s) 36, 107, 130, 344
BshNI GGYRCC 1 cut(s) 445
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 4 cut(s) 131, 176, 239, 824
BslFI GGGAC 5 cut(s) 287, 447, 591, 639, 727
BslI CCNNNNNNNGG 3 cut(s) 316, 711, 733
BsmAI GTCTC 2 cut(s) 17, 522
BsmFI GGGAC 5 cut(s) 287, 447, 591, 639, 727
BsnI GGCC 4 cut(s) 36, 107, 130, 344
Bso31I GGTCTC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 610
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 488
BspACI CCGC 4 cut(s) 54, 93, 126, 182
BspANI GGCC 4 cut(s) 36, 107, 130, 344
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 3 cut(s) 61, 254, 630
BspLI GGNNCC 3 cut(s) 294, 447, 463
BspMI ACCTGC 1 cut(s) 440
BspQI GCTCTTC 1 cut(s) 116
BspT104I TTCGAA 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 445
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 559
BsrFI RCCGGY 2 cut(s) 130, 175
BsrI ACTGG 2 cut(s) 275, 310
BssAI RCCGGY 2 cut(s) 130, 175
BssECI CCNNGG 2 cut(s) 108, 609
BssMI GATC 1 cut(s) 488
BssNI GRCGYC 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 4 cut(s) 319, 605, 679, 718
Bst6I CTCTTC 3 cut(s) 20, 49, 116
BstACI GRCGYC 1 cut(s) 558
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 4 cut(s) 22, 128, 252, 788
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 553, 768
BstFNI CGCG 3 cut(s) 61, 254, 630
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 2 cut(s) 167, 577
BstKTI GATC 1 cut(s) 491
BstMAI GTCTC 2 cut(s) 17, 522
BstMBI GATC 1 cut(s) 488
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNI CCWGG 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 471
BstUI CGCG 3 cut(s) 61, 254, 630
BstV1I GCAGC 4 cut(s) 149, 174, 795, 807
BstV2I GAAGAC 3 cut(s) 664, 707, 763
BstZI CGGCCG 1 cut(s) 105
Bsu36I CCTNAGG 1 cut(s) 372
BsuRI GGCC 4 cut(s) 36, 107, 130, 344
BtgZI GCGATG 1 cut(s) 156
BtrI CACGTC 4 cut(s) 75, 100, 232, 262
BtsCI GGATG 2 cut(s) 553, 768
BtsIMutI CAGTG 2 cut(s) 317, 597
BveI ACCTGC 1 cut(s) 440
Cac8I GCNNGC 4 cut(s) 22, 128, 252, 788
CaiI CAGNNNCTG 1 cut(s) 716
CfoI GCGC 2 cut(s) 167, 577
Cfr10I RCCGGY 2 cut(s) 130, 175
Cfr13I GGNCC 3 cut(s) 128, 292, 461
CseI GACGC 2 cut(s) 547, 636
Csp6I GTAC 1 cut(s) 256
CviAII CATG 2 cut(s) 280, 361
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 1 cut(s) 372
DpnI GATC 1 cut(s) 490
DpnII GATC 1 cut(s) 488
EaeI YGGCCR 1 cut(s) 105
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 3 cut(s) 20, 49, 116
EarI CTCTTC 3 cut(s) 20, 49, 116
EciI GGCGGA 1 cut(s) 197
EclXI CGGCCG 1 cut(s) 105
Eco147I AGGCCT 1 cut(s) 344
Eco31I GGTCTC 1 cut(s) 17
Eco47I GGWCC 2 cut(s) 292, 461
Eco52I CGGCCG 1 cut(s) 105
Eco81I CCTNAGG 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 610
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 471
FaeI CATG 2 cut(s) 283, 364
FaiI YATR 6 cut(s) 281, 300, 362, 582, 648, 733
FalI AAGNNNNNCTT 2 cut(s) 478, 510
FaqI GGGAC 5 cut(s) 287, 447, 591, 639, 727
FatI CATG 2 cut(s) 279, 360
FauI CCCGC 2 cut(s) 61, 119
Fnu4HI GCNGC 4 cut(s) 163, 188, 784, 821
FokI GGATG 2 cut(s) 560, 775
Fsp4HI GCNGC 4 cut(s) 163, 188, 784, 821
FspBI CTAG 1 cut(s) 450
GlaI GCGC 2 cut(s) 166, 576
GluI GCNGC 4 cut(s) 163, 188, 784, 821
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 4 cut(s) 36, 107, 130, 344
HapII CCGG 4 cut(s) 131, 176, 239, 824
HgaI GACGC 2 cut(s) 547, 636
HhaI GCGC 2 cut(s) 167, 577
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 2 cut(s) 283, 364
Hin6I GCGC 2 cut(s) 165, 575
HinP1I GCGC 2 cut(s) 165, 575
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 2 cut(s) 503, 660
HpaII CCGG 4 cut(s) 131, 176, 239, 824
Hpy166II GTNNAC 2 cut(s) 315, 366
Hpy188I TCNGA 2 cut(s) 502, 780
Hpy188III TCNNGA 1 cut(s) 612
Hpy8I GTNNAC 2 cut(s) 315, 366
Hpy99I CGWCG 2 cut(s) 157, 266
HpyAV CCTTC 2 cut(s) 283, 733
HpyCH4III ACNGT 4 cut(s) 319, 605, 679, 718
HpyCH4IV ACGT 5 cut(s) 74, 99, 231, 261, 467
HpyCH4V TGCA 3 cut(s) 435, 511, 675
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 1 cut(s) 372
HpySE526I ACGT 5 cut(s) 74, 99, 231, 261, 467
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 2 cut(s) 283, 364
HspAI GCGC 2 cut(s) 165, 575
Kzo9I GATC 1 cut(s) 488
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 4 cut(s) 112, 142, 184, 527
Lsp1109I GCAGC 4 cut(s) 149, 174, 795, 807
LweI GCATC 2 cut(s) 444, 799
MaeI CTAG 1 cut(s) 450
MaeII ACGT 5 cut(s) 74, 99, 231, 261, 467
MaeIII GTNAC 3 cut(s) 227, 595, 718
MalI GATC 1 cut(s) 490
MbiI CCGCTC 1 cut(s) 126
MboI GATC 1 cut(s) 488
MboII GAAGA 8 cut(s) 7, 36, 39, 133, 489, 669, 712, 763
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 2 cut(s) 331, 829
MluI ACGCGT 1 cut(s) 628
MlyI GAGTC 2 cut(s) 497, 654
MmeI TCCRAC 1 cut(s) 663
MseI TTAA 2 cut(s) 533, 681
MspA1I CMGCKG 2 cut(s) 95, 820
MspI CCGG 4 cut(s) 131, 176, 239, 824
MspR9I CCNGG 1 cut(s) 473
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 3 cut(s) 61, 254, 630
MwoI GCNNNNNNNGC 1 cut(s) 92
NdeII GATC 1 cut(s) 488
NlaIII CATG 2 cut(s) 283, 364
NlaIV GGNNCC 3 cut(s) 294, 447, 463
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 2 cut(s) 227, 595
NspV TTCGAA 1 cut(s) 483
PceI AGGCCT 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 4 cut(s) 105, 228, 258, 417
PkrI GCNGC 4 cut(s) 164, 189, 785, 822
PleI GAGTC 2 cut(s) 497, 654
PpsI GAGTC 2 cut(s) 497, 654
PpuMI RGGWCCY 1 cut(s) 292
Psp5II RGGWCCY 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 3 cut(s) 294, 447, 463
PspPI GGNCC 3 cut(s) 128, 292, 461
PspPPI RGGWCCY 1 cut(s) 292
PstNI CAGNNNCTG 1 cut(s) 716
PvuII CAGCTG 1 cut(s) 820
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 2 cut(s) 533, 681
SatI GCNGC 4 cut(s) 163, 188, 784, 821
Sau3AI GATC 1 cut(s) 488
Sau96I GGNCC 3 cut(s) 128, 292, 461
SchI GAGTC 2 cut(s) 497, 654
ScrFI CCNGG 1 cut(s) 473
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 2 cut(s) 444, 799
SfuI TTCGAA 1 cut(s) 483
SgrAI CRCCGGYG 1 cut(s) 175
SinI GGWCC 2 cut(s) 292, 461
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 2 cut(s) 331, 829
SseBI AGGCCT 1 cut(s) 344
SsiI CCGC 4 cut(s) 54, 93, 126, 182
SspMI CTAG 1 cut(s) 450
StuI AGGCCT 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 471
TaaI ACNGT 4 cut(s) 319, 605, 679, 718
TaiI ACGT 5 cut(s) 77, 102, 234, 264, 470
TaqI TCGA 5 cut(s) 197, 264, 305, 483, 663
TasI AATT 2 cut(s) 331, 829
Tru1I TTAA 2 cut(s) 533, 681
Tru9I TTAA 2 cut(s) 533, 681
TscAI CASTG 2 cut(s) 317, 604
TseFI GTSAC 2 cut(s) 227, 595
TseI GCWGC 4 cut(s) 162, 187, 783, 820
Tsp45I GTSAC 2 cut(s) 227, 595
TspDTI ATGAA 4 cut(s) 386, 429, 597, 763
TspRI CASTG 2 cut(s) 317, 604
VpaK11BI GGWCC 2 cut(s) 292, 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XspI CTAG 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.