MD11G1235500.v1.1

Protein of unknown function (DUF1223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013222
Physical Location & Seq
Reverse (-)
34265464 .. 34268248
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1235500.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGCGCGTGGTCTCCTCGCTTGCTTTGGATCAAAAGGAGGATCCCGCACGTCCCATGAAAAGCCTGCTGCGGGGGCAACAGCGCATGTCTCCGCTGAGGAGCAGCAGAGGAGCGGGCCGGTGCTGGTGGAGCTGTTCTCGTCGCAGGGCTGCAGCACCTCGCCGGTGGCAGAGCTGCTGGTGTCGAGGCTTGGGAGAGGGGATTTTGATGGGACGGACCTGCCTCCTGTGGTGGTGCTGGCGTTCCACGTGGACTACTGGGACTACATGGGCTGGAAGGACCCATATGGGTCGAGCCAGTGGACTGTACGGCAGAAGGCCTATGTCGAGGCCTTGGGTCTGGACACCATGTTCACCCCCCAGATTGTGCTTCAAGGTAGGGCCCAATGTGTTGGCAATGATGAGGGTGCCTTGCTAACCTCGATCAAGGATGCACCTAGATATCCTGCACCCGCATTCCAGGCAACATTCCAAAGGCCATCGCCGGACTCCATGCAAGTATCCCTAACAGGGTCATTGAGGTCAAAGGTGGACAACTACGGTGTCAATGTGATGGTGGCGCTGTACGAGAACGGGCTGATCACCGACTGCCCCAAGGGAGAGAACCAAGGACGTGTCCTGTCCAACGACTTCGTCGTCCGACGACTCGAAAAGCTCTGCACTGTCAAAGACATTGCTGCAAAGAAGACCATTTCCGGAACCATCAACTTCTCCTTGTGGGAAGGTTTCAATCCAGCCAAATGTAGCATGGCCCTCTTCGTTCAGAACCCTTCCCATCAAATTTTTGGTTTGCAGAACTTTCAGCTGCCGGACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1223

Protein Analysis

273

Amino Acids

29.6

Weight (kDa)

6.43

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1223 PF06764 42 - 255 4.7e-63 Protein of unknown function (DUF1223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27350 AT5G54240
fragaria_vesca FvH4_3g21110
malus_domestica MD03G1217700.v1.1 MD11G1235500.v1.1
prunus_persica Prupe.4G191500_v2.0.a1
pyrus_communis pycom03g16730 pycom1049g00020
rosa_chinensis RchiOBHm_Chr5g0036121
rosa_laevigata RLG00000033672
rosa_multiflora Rmu_sc0000382.1_g000011
rosa_roxburghii Rroxscaffold_1G00044620
rosa_rugosa Rorug05G0152700
rosa_samantha Rh5AG247200 Rh5BG247600 Rh5CG278500 Rh5DG255900
rosa_wichuraiana Rw5G022670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 407
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 695
AciI CCGC 5 cut(s) 46, 71, 93, 114, 453
AclWI GGATC 3 cut(s) 36, 37, 49
AcsI RAATTY 1 cut(s) 780
AcvI CACGTG 1 cut(s) 250
AfaI GTAC 2 cut(s) 309, 566
AfiI CCNNNNNNNGG 2 cut(s) 71, 510
AflIII ACRYGT 1 cut(s) 613
AgsI TTSAA 2 cut(s) 374, 730
AjiI CACGTC 2 cut(s) 51, 614
AjnI CCWGG 1 cut(s) 459
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 4 cut(s) 133, 175, 655, 805
AluI AGCT 4 cut(s) 133, 175, 655, 805
Alw26I GTCTC 2 cut(s) 17, 94
AlwI GGATC 3 cut(s) 36, 37, 49
Aor13HI TCCGGA 1 cut(s) 695
AoxI GGCC 6 cut(s) 116, 318, 330, 381, 476, 750
ApaI GGGCCC 1 cut(s) 385
ApeKI GCWGC 7 cut(s) 68, 103, 150, 153, 175, 677, 805
ApoI RAATTY 1 cut(s) 780
AspLEI GCGC 3 cut(s) 7, 85, 562
AspS9I GGNCC 6 cut(s) 116, 217, 280, 381, 382, 751
AsuHPI GGTGA 2 cut(s) 346, 574
AvaII GGWCC 2 cut(s) 217, 280
BaeGI GKGCMC 1 cut(s) 385
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 407
BanII GRGCYC 1 cut(s) 385
BbrPI CACGTG 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 692
BbvCI CCTCAGC 1 cut(s) 96
BbvI GCAGC 7 cut(s) 55, 115, 137, 162, 165, 664, 792
BccI CCATC 5 cut(s) 203, 487, 547, 710, 783
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 1 cut(s) 461
BciVI GTATCC 1 cut(s) 511
BclI TGATCA 1 cut(s) 579
BcoDI GTCTC 2 cut(s) 17, 94
BfaI CTAG 1 cut(s) 438
BfmI CTRYAG 1 cut(s) 151
BfoI RGCGCY 1 cut(s) 563
BfuAI ACCTGC 1 cut(s) 228
BfuI GTATCC 1 cut(s) 511
BisI GCNGC 7 cut(s) 69, 104, 151, 154, 176, 678, 806
BlsI GCNGC 7 cut(s) 70, 105, 152, 155, 177, 679, 807
Bme1390I CCNGG 1 cut(s) 461
Bme18I GGWCC 2 cut(s) 217, 280
BmgBI CACGTC 2 cut(s) 51, 614
BmgT120I GGNCC 6 cut(s) 116, 217, 280, 381, 382, 751
BmiI GGNNCC 5 cut(s) 43, 282, 383, 409, 700
BmrFI CCNGG 1 cut(s) 461
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 1 cut(s) 421
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 692
BplI GAGNNNNNCTC 2 cut(s) 122, 154
Bpu10I CCTNAGC 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 250
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 333, 594, 607
BsaWI WCCGGW 1 cut(s) 695
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 510
Bse118I RCCGGY 2 cut(s) 118, 163
Bse1I ACTGG 2 cut(s) 263, 298
Bse3DI GCAATG 2 cut(s) 403, 672
BseAI TCCGGA 1 cut(s) 695
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 3 cut(s) 333, 594, 607
BseGI GGATG 1 cut(s) 436
BseLI CCNNNNNNNGG 2 cut(s) 71, 510
BseMI GCAATG 2 cut(s) 403, 672
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 263, 298
BseRI GAGGAG 3 cut(s) 5, 113, 124
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 7 cut(s) 55, 115, 137, 162, 165, 664, 792
BsgI GTGCAG 2 cut(s) 432, 643
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 6 cut(s) 118, 320, 332, 383, 478, 752
BshNI GGYRCC 1 cut(s) 407
BsiSI CCGG 5 cut(s) 119, 164, 485, 696, 809
BslFI GGGAC 3 cut(s) 37, 226, 275
BslI CCNNNNNNNGG 2 cut(s) 71, 510
BsmAI GTCTC 2 cut(s) 17, 94
BsmFI GGGAC 3 cut(s) 37, 226, 275
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 6 cut(s) 118, 320, 332, 383, 478, 752
Bso31I GGTCTC 1 cut(s) 17
Bsp120I GGGCCC 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 385
Bsp13I TCCGGA 1 cut(s) 695
Bsp143I GATC 4 cut(s) 29, 41, 423, 579
BspACI CCGC 5 cut(s) 46, 71, 93, 114, 453
BspANI GGCC 6 cut(s) 118, 320, 332, 383, 478, 752
BspCNI CTCAG 1 cut(s) 88
BspEI TCCGGA 1 cut(s) 695
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 5 cut(s) 43, 282, 383, 409, 700
BspMAI CTGCAG 1 cut(s) 155
BspMI ACCTGC 1 cut(s) 228
BspPI GGATC 3 cut(s) 36, 37, 49
BspT107I GGYRCC 1 cut(s) 407
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 2 cut(s) 403, 672
BsrFI RCCGGY 2 cut(s) 118, 163
BsrI ACTGG 2 cut(s) 263, 298
BssAI RCCGGY 2 cut(s) 118, 163
BssECI CCNNGG 3 cut(s) 333, 594, 607
BssMI GATC 4 cut(s) 29, 41, 423, 579
BssT1I CCWWGG 3 cut(s) 333, 594, 607
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 3 cut(s) 307, 542, 664
Bst6I CTCTTC 1 cut(s) 761
BstBAI YACGTR 1 cut(s) 250
BstC8I GCNNGC 4 cut(s) 22, 66, 116, 240
BstDEI CTNAG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 436
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 3 cut(s) 7, 85, 562
BstKTI GATC 4 cut(s) 32, 44, 426, 582
BstMAI GTCTC 2 cut(s) 17, 94
BstMBI GATC 4 cut(s) 29, 41, 423, 579
BstMWI GCNNNNNNNGC 3 cut(s) 74, 130, 461
BstNI CCWGG 1 cut(s) 461
BstNSI RCATGY 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 459
BstSFI CTRYAG 1 cut(s) 151
BstSLI GKGCMC 1 cut(s) 385
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 7 cut(s) 55, 115, 137, 162, 165, 664, 792
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 1 cut(s) 41
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 1 cut(s) 41
BsuI GTATCC 1 cut(s) 511
BsuRI GGCC 6 cut(s) 118, 320, 332, 383, 478, 752
BtgZI GCGATG 1 cut(s) 465
BtrI CACGTC 2 cut(s) 51, 614
BtsCI GGATG 1 cut(s) 436
BtsIMutI CAGTG 2 cut(s) 305, 660
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 4 cut(s) 22, 66, 116, 240
CfoI GCGC 3 cut(s) 7, 85, 562
Cfr10I RCCGGY 2 cut(s) 118, 163
Cfr13I GGNCC 6 cut(s) 116, 217, 280, 381, 382, 751
Csp6I GTAC 2 cut(s) 308, 565
CviAII CATG 6 cut(s) 56, 86, 268, 349, 493, 748
CviQI GTAC 2 cut(s) 308, 565
DdeI CTNAG 1 cut(s) 96
DpnI GATC 4 cut(s) 31, 43, 425, 581
DpnII GATC 4 cut(s) 29, 41, 423, 579
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
Eam1104I CTCTTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 761
Eco130I CCWWGG 3 cut(s) 333, 594, 607
Eco147I AGGCCT 2 cut(s) 320, 332
Eco24I GRGCYC 1 cut(s) 385
Eco31I GGTCTC 1 cut(s) 17
Eco32I GATATC 1 cut(s) 443
Eco47I GGWCC 2 cut(s) 217, 280
Eco72I CACGTG 1 cut(s) 250
EcoO109I RGGNCCY 2 cut(s) 280, 381
EcoRII CCWGG 1 cut(s) 459
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 3 cut(s) 333, 594, 607
EcoT38I GRGCYC 1 cut(s) 385
ErhI CCWWGG 3 cut(s) 333, 594, 607
FaeI CATG 6 cut(s) 59, 89, 271, 352, 496, 751
FaiI YATR 9 cut(s) 57, 87, 269, 286, 288, 324, 350, 494, 749
FaqI GGGAC 3 cut(s) 37, 226, 275
FatI CATG 6 cut(s) 55, 85, 267, 348, 492, 747
FauI CCCGC 4 cut(s) 53, 64, 107, 460
FauNDI CATATG 1 cut(s) 286
FbaI TGATCA 1 cut(s) 579
Fnu4HI GCNGC 7 cut(s) 69, 104, 151, 154, 176, 678, 806
FokI GGATG 1 cut(s) 443
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 7 cut(s) 69, 104, 151, 154, 176, 678, 806
FspBI CTAG 1 cut(s) 438
GlaI GCGC 3 cut(s) 6, 84, 561
GluI GCNGC 7 cut(s) 69, 104, 151, 154, 176, 678, 806
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 6 cut(s) 118, 320, 332, 383, 478, 752
HapII CCGG 5 cut(s) 119, 164, 485, 696, 809
HhaI GCGC 3 cut(s) 7, 85, 562
Hin1II CATG 6 cut(s) 59, 89, 271, 352, 496, 751
Hin6I GCGC 3 cut(s) 5, 83, 560
HinP1I GCGC 3 cut(s) 5, 83, 560
HinfI GANTC 2 cut(s) 488, 645
HpaII CCGG 5 cut(s) 119, 164, 485, 696, 809
HphI GGTGA 2 cut(s) 346, 574
Hpy166II GTNNAC 4 cut(s) 253, 303, 354, 532
Hpy188I TCNGA 2 cut(s) 641, 765
Hpy188III TCNNGA 2 cut(s) 341, 696
Hpy8I GTNNAC 4 cut(s) 253, 303, 354, 532
Hpy99I CGWCG 3 cut(s) 145, 638, 645
HpyAV CCTTC 4 cut(s) 271, 310, 716, 780
HpyCH4III ACNGT 3 cut(s) 307, 542, 664
HpyCH4IV ACGT 3 cut(s) 50, 249, 613
HpyCH4V TGCA 7 cut(s) 153, 434, 449, 496, 660, 680, 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 130, 461
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 3 cut(s) 50, 249, 613
Hsp92II CATG 6 cut(s) 59, 89, 271, 352, 496, 751
HspAI GCGC 3 cut(s) 5, 83, 560
Kpn2I TCCGGA 1 cut(s) 695
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 4 cut(s) 29, 41, 423, 579
LmnI GCTCC 3 cut(s) 100, 111, 130
Lsp1109I GCAGC 7 cut(s) 55, 115, 137, 162, 165, 664, 792
LweI GCATC 1 cut(s) 421
MaeI CTAG 1 cut(s) 438
MaeII ACGT 3 cut(s) 50, 249, 613
MalI GATC 4 cut(s) 31, 43, 425, 581
MbiI CCGCTC 1 cut(s) 114
MboI GATC 4 cut(s) 29, 41, 423, 579
MboII GAAGA 2 cut(s) 697, 748
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 1 cut(s) 780
MlyI GAGTC 2 cut(s) 482, 639
MmeI TCCRAC 2 cut(s) 648, 664
MroI TCCGGA 1 cut(s) 695
MspA1I CMGCKG 2 cut(s) 95, 805
MspI CCGG 5 cut(s) 119, 164, 485, 696, 809
MspR9I CCNGG 1 cut(s) 461
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 1 cut(s) 461
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 74, 130, 461
NdeI CATATG 1 cut(s) 286
NdeII GATC 4 cut(s) 29, 41, 423, 579
NlaIII CATG 6 cut(s) 59, 89, 271, 352, 496, 751
NlaIV GGNNCC 5 cut(s) 43, 282, 383, 409, 700
NspI RCATGY 1 cut(s) 89
PceI AGGCCT 2 cut(s) 320, 332
PcsI WCGNNNNNNNCGW 1 cut(s) 633
PctI GAATGC 1 cut(s) 455
PflFI GACNNNGTC 1 cut(s) 632
PkrI GCNGC 7 cut(s) 70, 105, 152, 155, 177, 679, 807
PleI GAGTC 2 cut(s) 482, 639
PmaCI CACGTG 1 cut(s) 250
PmlI CACGTG 1 cut(s) 250
PpsI GAGTC 2 cut(s) 482, 639
Ppu21I YACGTR 1 cut(s) 250
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 459
PspCI CACGTG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 5 cut(s) 43, 282, 383, 409, 700
PspOMI GGGCCC 1 cut(s) 381
PspPI GGNCC 6 cut(s) 116, 217, 280, 381, 382, 751
PspPPI RGGWCCY 1 cut(s) 280
PstI CTGCAG 1 cut(s) 155
PsuI RGATCY 1 cut(s) 41
PsyI GACNNNGTC 1 cut(s) 632
PvuII CAGCTG 1 cut(s) 805
RsaI GTAC 2 cut(s) 309, 566
RsaNI GTAC 2 cut(s) 308, 565
SatI GCNGC 7 cut(s) 69, 104, 151, 154, 176, 678, 806
Sau3AI GATC 4 cut(s) 29, 41, 423, 579
Sau96I GGNCC 6 cut(s) 116, 217, 280, 381, 382, 751
SchI GAGTC 2 cut(s) 482, 639
ScrFI CCNGG 1 cut(s) 461
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 1 cut(s) 421
SfcI CTRYAG 1 cut(s) 151
SgrAI CRCCGGYG 1 cut(s) 163
SinI GGWCC 2 cut(s) 217, 280
Sse9I AATT 1 cut(s) 780
SseBI AGGCCT 2 cut(s) 320, 332
SsiI CCGC 5 cut(s) 46, 71, 93, 114, 453
SspMI CTAG 1 cut(s) 438
StuI AGGCCT 2 cut(s) 320, 332
StyD4I CCNGG 1 cut(s) 459
StyI CCWWGG 3 cut(s) 333, 594, 607
TaaI ACNGT 3 cut(s) 307, 542, 664
TaiI ACGT 3 cut(s) 53, 252, 616
TaqI TCGA 5 cut(s) 185, 293, 327, 422, 648
TasI AATT 1 cut(s) 780
TscAI CASTG 2 cut(s) 305, 667
TseI GCWGC 7 cut(s) 68, 103, 150, 153, 175, 677, 805
TspDTI ATGAA 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 2 cut(s) 305, 667
Tth111I GACNNNGTC 1 cut(s) 632
VpaK11BI GGWCC 2 cut(s) 217, 280
XapI RAATTY 1 cut(s) 780
XceI RCATGY 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 2 cut(s) 745, 782
XspI CTAG 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.