pycom03g16730

Protein of unknown function (DUF1223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013222
Physical Location & Seq
Reverse (-)
17898921 .. 17900933
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16730.1

Sequence Viewer

Length: 819 bp
ATGGCACGTGGTCTCCTCTCCTGCTTTGGATCAAAAGGGGGCTCGGGCACGTCCCACGAAACGCACGCGGCCGGGGCGACCGCGCATGTTTCGGCAGAGGAGCAGCAGCGGAGCGGACCGGTTCTGGTGGAGCTGTTCTCGTCGCAGGGCTGCACCACCTCGCCTGTCGCGGAGCTGCTGGTGTCGAGGCTTGGGAGGGGGGATTTTGATGGGACGGACCTGCCTCCTGTGGTTGCGCTGGCCTTCCACGTGGACTACTGGGACTACATGGGCTGGAAGGACCCTTATGGGTCCAGCCAGTGGACTGTACGGCAGAAGGCCTATGTTGAGGCCTTGGGTCTGGACACCATGTTCACCCCCCAGATTGTGCTTCAAGGTAGGGCCCAATGTGTTGGCAACGATGAGAGTGCTTTGATAACGTCTATCAAGGATGCACCTAGATATCCTGCACCGGCGTTCCAGGCAACATTCCAAAGGCCATCACAGGCATCCTTGCAAGTATCCCTAACAGGATCATTGAGGTCGAAGGTGGACAACTACGGTGCCAATGTGATGGTGGCGCTGTACGAGAACGGCCTGATTACCGACTGCCCCAAGGGAGAGAACCAAGGACGTGTCCTGTCCAATGACTTCGTCGTCCGACGACTTGAAAAGCTCTGCACTGTCAAAGACATTGCTGCAAAGAAGACCATTTCTGGAACCATAAACTTCTCCTTGTGGGACGGTTACAATCCAGCCAAATGTGGCATGGCCCTCTTTGTTCAGAACCCTTCCCATCAAATTTTTGGTTTGCAGGACTTTCAGCTGCCGGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1223

Protein Analysis

273

Amino Acids

29.46

Weight (kDa)

5.95

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1223 PF06764 42 - 255 1.8e-63 Protein of unknown function (DUF1223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27350 AT5G54240
fragaria_vesca FvH4_3g21110
malus_domestica MD03G1217700.v1.1 MD11G1235500.v1.1
prunus_persica Prupe.4G191500_v2.0.a1
pyrus_communis pycom03g16730 pycom1049g00020
rosa_chinensis RchiOBHm_Chr5g0036121
rosa_laevigata RLG00000033672
rosa_multiflora Rmu_sc0000382.1_g000011
rosa_roxburghii Rroxscaffold_1G00044620
rosa_rugosa Rorug05G0152700
rosa_samantha Rh5AG247200 Rh5BG247600 Rh5CG278500 Rh5DG255900
rosa_wichuraiana Rw5G022670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 542
AccB7I CCANNNNNTGG 1 cut(s) 300
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 3 cut(s) 68, 83, 170
AciI CCGC 5 cut(s) 68, 81, 109, 114, 170
AclWI GGATC 2 cut(s) 37, 520
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 1 cut(s) 780
AcvI CACGTG 2 cut(s) 8, 250
AfaI GTAC 2 cut(s) 309, 566
AfiI CCNNNNNNNGG 1 cut(s) 300
AflIII ACRYGT 1 cut(s) 613
AgeI ACCGGT 1 cut(s) 118
AgsI TTSAA 2 cut(s) 374, 650
AjiI CACGTC 2 cut(s) 51, 614
AjnI CCWGG 1 cut(s) 459
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 4 cut(s) 133, 175, 655, 805
AluI AGCT 4 cut(s) 133, 175, 655, 805
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 2 cut(s) 37, 520
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 8 cut(s) 69, 240, 318, 330, 381, 476, 574, 750
ApaI GGGCCC 1 cut(s) 385
ApeKI GCWGC 6 cut(s) 103, 106, 150, 175, 677, 805
ApoI RAATTY 1 cut(s) 780
AsiGI ACCGGT 1 cut(s) 118
AspLEI GCGC 3 cut(s) 85, 238, 562
AspS9I GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 751
AsuC2I CCSGG 1 cut(s) 73
AsuHPI GGTGA 1 cut(s) 346
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 4 cut(s) 116, 217, 280, 291
BaeGI GKGCMC 2 cut(s) 50, 385
BanI GGYRCC 1 cut(s) 542
BanII GRGCYC 2 cut(s) 44, 385
BbrPI CACGTG 2 cut(s) 8, 250
BbsI GAAGAC 1 cut(s) 692
BbvI GCAGC 6 cut(s) 115, 118, 137, 162, 664, 792
BccI CCATC 4 cut(s) 203, 487, 547, 783
BceAI ACGGC 2 cut(s) 326, 589
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 461
BciVI GTATCC 1 cut(s) 511
BcnI CCSGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 563
BfuAI ACCTGC 1 cut(s) 228
BfuI GTATCC 1 cut(s) 511
BisI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 678, 806
BlsI GCNGC 7 cut(s) 70, 105, 108, 152, 177, 679, 807
Bme1390I CCNGG 2 cut(s) 73, 461
Bme18I GGWCC 4 cut(s) 116, 217, 280, 291
BmeT110I CYCGRG 1 cut(s) 43
BmgBI CACGTC 2 cut(s) 51, 614
BmgT120I GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 751
BmiI GGNNCC 5 cut(s) 282, 292, 383, 544, 700
BmrFI CCNGG 2 cut(s) 73, 461
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 2 cut(s) 421, 497
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 692
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpuMI CCSGG 1 cut(s) 73
BsaAI YACGTR 2 cut(s) 8, 250
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 72, 333, 594, 607
BsaWI WCCGGW 1 cut(s) 118
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 300
Bse118I RCCGGY 2 cut(s) 118, 451
Bse1I ACTGG 2 cut(s) 263, 298
Bse3DI GCAATG 1 cut(s) 672
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 4 cut(s) 72, 333, 594, 607
BseGI GGATG 2 cut(s) 436, 488
BseLI CCNNNNNNNGG 1 cut(s) 300
BseMI GCAATG 1 cut(s) 672
BseNI ACTGG 2 cut(s) 263, 298
BseRI GAGGAG 2 cut(s) 5, 113
BseSI GKGCMC 2 cut(s) 50, 385
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 6 cut(s) 115, 118, 137, 162, 664, 792
BsgI GTGCAG 3 cut(s) 136, 432, 643
Bsh1236I CGCG 3 cut(s) 68, 83, 170
Bsh1285I CGRYCG 2 cut(s) 72, 81
BshFI GGCC 8 cut(s) 71, 242, 320, 332, 383, 478, 576, 752
BshNI GGYRCC 1 cut(s) 542
BshTI ACCGGT 1 cut(s) 118
BsiEI CGRYCG 2 cut(s) 72, 81
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 4 cut(s) 72, 119, 452, 809
BslFI GGGAC 4 cut(s) 37, 226, 275, 734
BslI CCNNNNNNNGG 1 cut(s) 300
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 4 cut(s) 37, 226, 275, 734
BsnI GGCC 8 cut(s) 71, 242, 320, 332, 383, 478, 576, 752
Bso31I GGTCTC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 43
Bsp120I GGGCCC 1 cut(s) 381
Bsp1286I GDGCHC 3 cut(s) 44, 50, 385
Bsp143I GATC 2 cut(s) 29, 512
BspACI CCGC 5 cut(s) 68, 81, 109, 114, 170
BspANI GGCC 8 cut(s) 71, 242, 320, 332, 383, 478, 576, 752
BspFNI CGCG 3 cut(s) 68, 83, 170
BspLI GGNNCC 5 cut(s) 282, 292, 383, 544, 700
BspMI ACCTGC 1 cut(s) 228
BspPI GGATC 2 cut(s) 37, 520
BspT107I GGYRCC 1 cut(s) 542
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 672
BsrFI RCCGGY 2 cut(s) 118, 451
BsrI ACTGG 2 cut(s) 263, 298
BssAI RCCGGY 2 cut(s) 118, 451
BssECI CCNNGG 4 cut(s) 72, 333, 594, 607
BssMI GATC 2 cut(s) 29, 512
BssT1I CCWWGG 3 cut(s) 333, 594, 607
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 4 cut(s) 307, 542, 664, 725
BstBAI YACGTR 2 cut(s) 8, 250
BstC8I GCNNGC 2 cut(s) 66, 240
BstF5I GGATG 2 cut(s) 436, 488
BstFNI CGCG 3 cut(s) 68, 83, 170
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 3 cut(s) 85, 238, 562
BstKTI GATC 2 cut(s) 32, 515
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 2 cut(s) 29, 512
BstMCI CGRYCG 2 cut(s) 72, 81
BstMWI GCNNNNNNNGC 2 cut(s) 74, 461
BstNI CCWGG 1 cut(s) 461
BstNSI RCATGY 1 cut(s) 89
BstSCI CCNGG 2 cut(s) 71, 459
BstSLI GKGCMC 2 cut(s) 50, 385
BstUI CGCG 3 cut(s) 68, 83, 170
BstV1I GCAGC 6 cut(s) 115, 118, 137, 162, 664, 792
BstV2I GAAGAC 1 cut(s) 692
BstXI CCANNNNNNTGG 2 cut(s) 392, 553
BstZI CGGCCG 1 cut(s) 69
BsuI GTATCC 1 cut(s) 511
BsuRI GGCC 8 cut(s) 71, 242, 320, 332, 383, 478, 576, 752
BtrI CACGTC 2 cut(s) 51, 614
BtsCI GGATG 2 cut(s) 436, 488
BtsIMutI CAGTG 2 cut(s) 305, 660
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 2 cut(s) 66, 240
CfoI GCGC 3 cut(s) 85, 238, 562
Cfr10I RCCGGY 2 cut(s) 118, 451
Cfr13I GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 751
CpoI CGGWCCG 1 cut(s) 116
Csp6I GTAC 2 cut(s) 308, 565
CspAI ACCGGT 1 cut(s) 118
CspI CGGWCCG 1 cut(s) 116
CviAII CATG 4 cut(s) 86, 268, 349, 748
CviQI GTAC 2 cut(s) 308, 565
DpnI GATC 2 cut(s) 31, 514
DpnII GATC 2 cut(s) 29, 512
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 333, 594, 607
Eco147I AGGCCT 2 cut(s) 320, 332
Eco24I GRGCYC 2 cut(s) 44, 385
Eco31I GGTCTC 1 cut(s) 17
Eco32I GATATC 1 cut(s) 443
Eco47I GGWCC 4 cut(s) 116, 217, 280, 291
Eco52I CGGCCG 1 cut(s) 69
Eco72I CACGTG 2 cut(s) 8, 250
Eco88I CYCGRG 1 cut(s) 43
EcoO109I RGGNCCY 2 cut(s) 280, 381
EcoRII CCWGG 1 cut(s) 459
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 3 cut(s) 333, 594, 607
EcoT38I GRGCYC 2 cut(s) 44, 385
ErhI CCWWGG 3 cut(s) 333, 594, 607
FaeI CATG 4 cut(s) 89, 271, 352, 751
FaiI YATR 7 cut(s) 87, 269, 288, 324, 350, 704, 749
FaqI GGGAC 4 cut(s) 37, 226, 275, 734
FatI CATG 4 cut(s) 85, 267, 348, 747
Fnu4HI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 678, 806
FokI GGATG 2 cut(s) 443, 475
FriOI GRGCYC 2 cut(s) 44, 385
Fsp4HI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 678, 806
FspBI CTAG 1 cut(s) 438
GlaI GCGC 3 cut(s) 84, 237, 561
GluI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 678, 806
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 8 cut(s) 71, 242, 320, 332, 383, 478, 576, 752
HapII CCGG 4 cut(s) 72, 119, 452, 809
HhaI GCGC 3 cut(s) 85, 238, 562
Hin1II CATG 4 cut(s) 89, 271, 352, 751
Hin6I GCGC 3 cut(s) 83, 236, 560
HinP1I GCGC 3 cut(s) 83, 236, 560
HpaII CCGG 4 cut(s) 72, 119, 452, 809
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 4 cut(s) 253, 303, 354, 532
Hpy188I TCNGA 2 cut(s) 641, 765
Hpy188III TCNNGA 2 cut(s) 341, 696
Hpy8I GTNNAC 4 cut(s) 253, 303, 354, 532
Hpy99I CGWCG 3 cut(s) 145, 638, 645
HpyAV CCTTC 5 cut(s) 253, 271, 310, 520, 780
HpyCH4III ACNGT 4 cut(s) 307, 542, 664, 725
HpyCH4IV ACGT 5 cut(s) 7, 50, 249, 419, 613
HpyCH4V TGCA 7 cut(s) 153, 434, 449, 496, 660, 680, 793
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 461
HpySE526I ACGT 5 cut(s) 7, 50, 249, 419, 613
Hsp92II CATG 4 cut(s) 89, 271, 352, 751
HspAI GCGC 3 cut(s) 83, 236, 560
Kzo9I GATC 2 cut(s) 29, 512
LmnI GCTCC 4 cut(s) 100, 111, 130, 172
Lsp1109I GCAGC 6 cut(s) 115, 118, 137, 162, 664, 792
LweI GCATC 2 cut(s) 421, 497
MaeI CTAG 1 cut(s) 438
MaeII ACGT 5 cut(s) 7, 50, 249, 419, 613
MaeIII GTNAC 1 cut(s) 725
MalI GATC 2 cut(s) 31, 514
MbiI CCGCTC 1 cut(s) 114
MboI GATC 2 cut(s) 29, 512
MboII GAAGA 1 cut(s) 697
MfeI CAATTG 1 cut(s) 814
MhlI GDGCHC 3 cut(s) 44, 50, 385
MluCI AATT 2 cut(s) 780, 814
MmeI TCCRAC 1 cut(s) 664
MnlI CCTC 9 cut(s) 26, 91, 169, 180, 189, 234, 322, 513, 764
MspA1I CMGCKG 2 cut(s) 109, 805
MspI CCGG 4 cut(s) 72, 119, 452, 809
MspR9I CCNGG 2 cut(s) 73, 461
MunI CAATTG 1 cut(s) 814
MvaI CCWGG 1 cut(s) 461
MvnI CGCG 3 cut(s) 68, 83, 170
MwoI GCNNNNNNNGC 2 cut(s) 74, 461
NciI CCSGG 1 cut(s) 73
NdeII GATC 2 cut(s) 29, 512
NlaIII CATG 4 cut(s) 89, 271, 352, 751
NlaIV GGNNCC 5 cut(s) 282, 292, 383, 544, 700
NspI RCATGY 1 cut(s) 89
PceI AGGCCT 2 cut(s) 320, 332
PflFI GACNNNGTC 1 cut(s) 632
PflMI CCANNNNNTGG 1 cut(s) 300
PinAI ACCGGT 1 cut(s) 118
PkrI GCNGC 7 cut(s) 70, 105, 108, 152, 177, 679, 807
PmaCI CACGTG 2 cut(s) 8, 250
PmlI CACGTG 2 cut(s) 8, 250
Ppu21I YACGTR 2 cut(s) 8, 250
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 459
PspCI CACGTG 2 cut(s) 8, 250
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 5 cut(s) 282, 292, 383, 544, 700
PspOMI GGGCCC 1 cut(s) 381
PspPI GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 751
PspPPI RGGWCCY 1 cut(s) 280
PsyI GACNNNGTC 1 cut(s) 632
PvuII CAGCTG 1 cut(s) 805
RsaI GTAC 2 cut(s) 309, 566
RsaNI GTAC 2 cut(s) 308, 565
Rsr2I CGGWCCG 1 cut(s) 116
RsrII CGGWCCG 1 cut(s) 116
SatI GCNGC 7 cut(s) 69, 104, 107, 151, 176, 678, 806
Sau3AI GATC 2 cut(s) 29, 512
Sau96I GGNCC 7 cut(s) 116, 217, 280, 291, 381, 382, 751
ScrFI CCNGG 2 cut(s) 73, 461
SduI GDGCHC 3 cut(s) 44, 50, 385
SfaNI GCATC 2 cut(s) 421, 497
SgrAI CRCCGGYG 1 cut(s) 451
SinI GGWCC 4 cut(s) 116, 217, 280, 291
Sse9I AATT 2 cut(s) 780, 814
SseBI AGGCCT 2 cut(s) 320, 332
SsiI CCGC 5 cut(s) 68, 81, 109, 114, 170
SspMI CTAG 1 cut(s) 438
StuI AGGCCT 2 cut(s) 320, 332
StyD4I CCNGG 2 cut(s) 71, 459
StyI CCWWGG 3 cut(s) 333, 594, 607
TaaI ACNGT 4 cut(s) 307, 542, 664, 725
TaiI ACGT 5 cut(s) 10, 53, 252, 422, 616
TaqI TCGA 2 cut(s) 185, 524
TasI AATT 2 cut(s) 780, 814
TauI GCSGC 1 cut(s) 71
TscAI CASTG 2 cut(s) 305, 667
TseI GCWGC 6 cut(s) 103, 106, 150, 175, 677, 805
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 2 cut(s) 305, 667
Tth111I GACNNNGTC 1 cut(s) 632
Van91I CCANNNNNTGG 1 cut(s) 300
VpaK11BI GGWCC 4 cut(s) 116, 217, 280, 291
XapI RAATTY 1 cut(s) 780
XceI RCATGY 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 3 cut(s) 553, 745, 782
XspI CTAG 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.