AT5G54240

Protein of unknown function (DUF1223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013222
Physical Location & Seq
Reverse (-)
22022978 .. 22024658
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54240.1

Sequence Viewer

Length: 849 bp
ATGGCGCCTCGTCTTTTCACATGTTTTGGCGTCAAAGGATCTTCAACTTCCACCACCACCGGAAAAGGTATTTCCGGTCATAACGCGGCTAATGTAGCTGCTGATGTTCCGGTAGGTGATGGACCTATTCTTGTTCAGCTTTTCTCATCTCAAGGATGTAAAACTTCGCCTGAGGCGGAGATGCTTGTATCACGTCTTGGACGTGGTGATTTCGATGGAAAGATCCGTCGAGACGGTGGATCTAGTGGTTCTCCGGCGATGGTTTTGGTTTTTCACGTTGATTATTGGGATTATCTTGGATGGAAAGATCCTTATGGATCGAGTCAGTGGACCGTGAGACAAAAGGCCTATATCGAGGCTTTGAATCAAGATACAATGTTTACTCCACAGTTTGTTGTTCAAGGTCGTGTTCAATTACTTGGGAATGAAGAAGAGTCCTTGCTTAAGTCTATTGTTGAAGCTCCTAGGTTTCCGTCTCCTGCGTTTCGGGCCACATTTCAAAGACCAACATCAGAAACCCTTCAAGTATCTCTCACCGGAGCTTTGAGGATGAAAGTGGACTCGAGCGGGATCGATATAATGGTGGCGCTATATGAAAACGGTTTGGTGAATGATTGCGCTCGAGGAGAGAACTCAGGAAGAGTCTTGTCCAATGACTACGTTGTAAGAAAGCTAGAAAAGTTATGCACCGTTAAAGATTTAACGGGAAAAAAGACGGTGTCAGGGACAGCTCATTTCACGGTTTGGGATGGATTCAACAGCTCTAAATGTGGAGTCGCTGTTTTTCTACAGAACACATCTCTTCAAATTTTTGGTACGCAGAGTTTTCAATTGCCTGATGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1223

Protein Analysis

282

Amino Acids

30.63

Weight (kDa)

6.83

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1223 PF06764 44 - 264 2.7e-60 Protein of unknown function (DUF1223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27350 AT5G54240
fragaria_vesca FvH4_3g21110
malus_domestica MD03G1217700.v1.1 MD11G1235500.v1.1
prunus_persica Prupe.4G191500_v2.0.a1
pyrus_communis pycom03g16730 pycom1049g00020
rosa_chinensis RchiOBHm_Chr5g0036121
rosa_laevigata RLG00000033672
rosa_multiflora Rmu_sc0000382.1_g000011
rosa_roxburghii Rroxscaffold_1G00044620
rosa_rugosa Rorug05G0152700
rosa_samantha Rh5AG247200 Rh5BG247600 Rh5CG278500 Rh5DG255900
rosa_wichuraiana Rw5G022670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 567
AccII CGCG 1 cut(s) 86
AciI CCGC 3 cut(s) 86, 176, 567
AclWI GGATC 6 cut(s) 46, 217, 247, 302, 325, 578
AcsI RAATTY 1 cut(s) 807
AcyI GRCGYC 2 cut(s) 5, 30
AfaI GTAC 1 cut(s) 817
AflII CTTAAG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 20
AjiI CACGTC 2 cut(s) 194, 203
AluBI AGCT 7 cut(s) 98, 139, 461, 542, 673, 731, 762
AluI AGCT 7 cut(s) 98, 139, 461, 542, 673, 731, 762
Alw26I GTCTC 3 cut(s) 225, 331, 480
AlwI GGATC 6 cut(s) 46, 217, 247, 302, 325, 578
Ama87I CYCGRG 2 cut(s) 562, 621
AoxI GGCC 2 cut(s) 345, 489
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 1 cut(s) 807
Asp700I GAANNNNTTC 1 cut(s) 519
AspA2I CCTAGG 1 cut(s) 464
AspLEI GCGC 3 cut(s) 7, 589, 620
AspS9I GGNCC 3 cut(s) 122, 330, 489
AsuHPI GGTGA 4 cut(s) 128, 218, 526, 619
AvaI CYCGRG 2 cut(s) 562, 621
AvaII GGWCC 2 cut(s) 122, 330
AvrII CCTAGG 1 cut(s) 464
AxyI CCTNAGG 1 cut(s) 171
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 1 cut(s) 85
BccI CCATC 5 cut(s) 113, 209, 253, 294, 743
BcoDI GTCTC 3 cut(s) 225, 331, 480
BfaI CTAG 3 cut(s) 243, 465, 674
BfmI CTRYAG 1 cut(s) 788
BfoI RGCGCY 2 cut(s) 8, 590
BfrI CTTAAG 1 cut(s) 443
BisI GCNGC 2 cut(s) 87, 99
BlnI CCTAGG 1 cut(s) 464
BlsI GCNGC 2 cut(s) 88, 100
Bme18I GGWCC 2 cut(s) 122, 330
BmeT110I CYCGRG 2 cut(s) 562, 621
BmgBI CACGTC 2 cut(s) 194, 203
BmgT120I GGNCC 3 cut(s) 122, 330, 489
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 573
BsaHI GRCGYC 2 cut(s) 5, 30
BsaJI CCNNGG 1 cut(s) 464
BsaWI WCCGGW 4 cut(s) 59, 74, 109, 536
BsaXI ACNNNNNCTCC 2 cut(s) 765, 795
Bse21I CCTNAGG 1 cut(s) 171
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 464
BseGI GGATG 4 cut(s) 161, 305, 555, 754
BseMII CTCAG 2 cut(s) 162, 648
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 1 cut(s) 85
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 2 cut(s) 347, 491
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 1 cut(s) 573
BsiHKCI CYCGRG 2 cut(s) 562, 621
BsiSI CCGG 5 cut(s) 60, 75, 110, 254, 537
BslFI GGGAC 1 cut(s) 739
BsmAI GTCTC 3 cut(s) 225, 331, 480
BsmBI CGTCTC 2 cut(s) 225, 480
BsmFI GGGAC 1 cut(s) 739
BsnI GGCC 2 cut(s) 347, 491
BsoBI CYCGRG 2 cut(s) 562, 621
Bsp143I GATC 6 cut(s) 38, 222, 239, 307, 317, 570
BspACI CCGC 3 cut(s) 86, 176, 567
BspANI GGCC 2 cut(s) 347, 491
BspCNI CTCAG 2 cut(s) 163, 647
BspDI ATCGAT 1 cut(s) 573
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 6 cut(s) 46, 217, 247, 302, 325, 578
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 567
BssECI CCNNGG 1 cut(s) 464
BssMI GATC 6 cut(s) 38, 222, 239, 307, 317, 570
BssNI GRCGYC 2 cut(s) 5, 30
BssT1I CCWWGG 1 cut(s) 464
Bst4CI ACNGT 7 cut(s) 236, 334, 390, 602, 691, 718, 742
Bst6I CTCTTC 3 cut(s) 426, 634, 807
BstACI GRCGYC 2 cut(s) 5, 30
BstAFI CTTAAG 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 171, 634
BstF5I GGATG 4 cut(s) 161, 305, 555, 754
BstFNI CGCG 1 cut(s) 86
BstH2I RGCGCY 2 cut(s) 8, 590
BstHHI GCGC 3 cut(s) 7, 589, 620
BstKTI GATC 6 cut(s) 41, 225, 242, 310, 320, 573
BstMAI GTCTC 3 cut(s) 225, 331, 480
BstMBI GATC 6 cut(s) 38, 222, 239, 307, 317, 570
BstMWI GCNNNNNNNGC 2 cut(s) 95, 488
BstNSI RCATGY 1 cut(s) 24
BstSFI CTRYAG 1 cut(s) 788
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 85
BstX2I RGATCY 4 cut(s) 38, 222, 239, 307
BstYI RGATCY 4 cut(s) 38, 222, 239, 307
Bsu15I ATCGAT 1 cut(s) 573
Bsu36I CCTNAGG 1 cut(s) 171
BsuRI GGCC 2 cut(s) 347, 491
BsuTUI ATCGAT 1 cut(s) 573
BtgZI GCGATG 1 cut(s) 272
BtrI CACGTC 2 cut(s) 194, 203
BtsCI GGATG 4 cut(s) 161, 305, 555, 754
BtsIMutI CAGTG 1 cut(s) 332
CfoI GCGC 3 cut(s) 7, 589, 620
Cfr13I GGNCC 3 cut(s) 122, 330, 489
ClaI ATCGAT 1 cut(s) 573
CseI GACGC 1 cut(s) 19
Csp6I GTAC 1 cut(s) 816
CviAII CATG 1 cut(s) 21
CviQI GTAC 1 cut(s) 816
DdeI CTNAG 2 cut(s) 171, 634
DinI GGCGCC 1 cut(s) 6
DpnI GATC 6 cut(s) 40, 224, 241, 309, 319, 572
DpnII GATC 6 cut(s) 38, 222, 239, 307, 317, 570
Eam1104I CTCTTC 3 cut(s) 426, 634, 807
EarI CTCTTC 3 cut(s) 426, 634, 807
EciI GGCGGA 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 464
Eco147I AGGCCT 1 cut(s) 347
Eco47I GGWCC 2 cut(s) 122, 330
Eco81I CCTNAGG 1 cut(s) 171
Eco88I CYCGRG 2 cut(s) 562, 621
EcoT14I CCWWGG 1 cut(s) 464
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 464
Esp3I CGTCTC 2 cut(s) 225, 480
FaeI CATG 1 cut(s) 24
FaiI YATR 8 cut(s) 22, 81, 315, 351, 578, 592, 594, 685
FaqI GGGAC 1 cut(s) 739
FatI CATG 1 cut(s) 20
FauI CCCGC 1 cut(s) 560
Fnu4HI GCNGC 2 cut(s) 87, 99
FokI GGATG 4 cut(s) 168, 312, 562, 761
Fsp4HI GCNGC 2 cut(s) 87, 99
FspBI CTAG 3 cut(s) 243, 465, 674
GlaI GCGC 3 cut(s) 6, 588, 619
GluI GCNGC 2 cut(s) 87, 99
HaeII RGCGCY 2 cut(s) 8, 590
HaeIII GGCC 2 cut(s) 347, 491
HapII CCGG 5 cut(s) 60, 75, 110, 254, 537
HgaI GACGC 1 cut(s) 19
HhaI GCGC 3 cut(s) 7, 589, 620
Hin1I GRCGYC 2 cut(s) 5, 30
Hin1II CATG 1 cut(s) 24
Hin6I GCGC 3 cut(s) 5, 587, 618
HinP1I GCGC 3 cut(s) 5, 587, 618
HinfI GANTC 7 cut(s) 322, 364, 434, 560, 642, 753, 774
HpaII CCGG 5 cut(s) 60, 75, 110, 254, 537
HphI GGTGA 4 cut(s) 128, 218, 526, 619
Hpy166II GTNNAC 3 cut(s) 330, 381, 559
Hpy188I TCNGA 1 cut(s) 514
Hpy188III TCNNGA 3 cut(s) 230, 368, 636
Hpy8I GTNNAC 3 cut(s) 330, 381, 559
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 1 cut(s) 530
HpyCH4III ACNGT 7 cut(s) 236, 334, 390, 602, 691, 718, 742
HpyCH4IV ACGT 4 cut(s) 193, 202, 276, 660
HpyCH4V TGCA 1 cut(s) 687
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 488
HpyF3I CTNAG 2 cut(s) 171, 634
HpySE526I ACGT 4 cut(s) 193, 202, 276, 660
Hsp92I GRCGYC 2 cut(s) 5, 30
Hsp92II CATG 1 cut(s) 24
HspAI GCGC 3 cut(s) 5, 587, 618
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 6 cut(s) 38, 222, 239, 307, 317, 570
LmnI GCTCC 2 cut(s) 466, 539
LpnPI CCDG 9 cut(s) 73, 88, 123, 183, 267, 492, 550, 621, 708
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 1 cut(s) 171
MaeI CTAG 3 cut(s) 243, 465, 674
MaeII ACGT 4 cut(s) 193, 202, 276, 660
MalI GATC 6 cut(s) 40, 224, 241, 309, 319, 572
MbiI CCGCTC 1 cut(s) 567
MboI GATC 6 cut(s) 38, 222, 239, 307, 317, 570
MboII GAAGA 5 cut(s) 33, 440, 443, 651, 794
MfeI CAATTG 1 cut(s) 830
MflI RGATCY 4 cut(s) 38, 222, 239, 307
MluCI AATT 3 cut(s) 413, 807, 830
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 5 cut(s) 331, 443, 554, 651, 783
MnlI CCTC 5 cut(s) 18, 166, 349, 540, 617
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 3 cut(s) 444, 693, 701
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 5 cut(s) 60, 75, 110, 254, 537
MunI CAATTG 1 cut(s) 830
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 95, 488
NarI GGCGCC 1 cut(s) 5
NdeII GATC 6 cut(s) 38, 222, 239, 307, 317, 570
NlaIII CATG 1 cut(s) 24
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 24
PaeR7I CTCGAG 2 cut(s) 562, 621
PceI AGGCCT 1 cut(s) 347
PciI ACATGT 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 2 cut(s) 364, 753
PflFI GACNNNGTC 1 cut(s) 718
PkrI GCNGC 2 cut(s) 88, 100
PleI GAGTC 5 cut(s) 330, 442, 554, 650, 782
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 5 cut(s) 330, 442, 554, 650, 782
PscI ACATGT 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 122, 330, 489
PspXI VCTCGAGB 2 cut(s) 562, 621
PsuI RGATCY 4 cut(s) 38, 222, 239, 307
PsyI GACNNNGTC 1 cut(s) 718
RsaI GTAC 1 cut(s) 817
RsaNI GTAC 1 cut(s) 816
SaqAI TTAA 3 cut(s) 444, 693, 701
SatI GCNGC 2 cut(s) 87, 99
Sau3AI GATC 6 cut(s) 38, 222, 239, 307, 317, 570
Sau96I GGNCC 3 cut(s) 122, 330, 489
SchI GAGTC 5 cut(s) 331, 443, 554, 651, 783
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 1 cut(s) 788
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 2 cut(s) 562, 621
SinI GGWCC 2 cut(s) 122, 330
SlaI CTCGAG 2 cut(s) 562, 621
SmlI CTYRAG 4 cut(s) 150, 443, 562, 621
SmoI CTYRAG 4 cut(s) 150, 443, 562, 621
Sse9I AATT 3 cut(s) 413, 807, 830
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 3 cut(s) 86, 176, 567
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 243, 465, 674
StuI AGGCCT 1 cut(s) 347
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 7 cut(s) 236, 334, 390, 602, 691, 718, 742
TaiI ACGT 4 cut(s) 196, 205, 279, 663
TaqI TCGA 7 cut(s) 213, 229, 320, 354, 563, 573, 622
TasI AATT 3 cut(s) 413, 807, 830
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 2 cut(s) 364, 753
Tru1I TTAA 3 cut(s) 444, 693, 701
Tru9I TTAA 3 cut(s) 444, 693, 701
TscAI CASTG 1 cut(s) 332
TseI GCWGC 1 cut(s) 98
TspDTI ATGAA 3 cut(s) 441, 566, 609
TspGWI ACGGA 2 cut(s) 215, 462
TspRI CASTG 1 cut(s) 332
Tth111I GACNNNGTC 1 cut(s) 718
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 2 cut(s) 122, 330
XapI RAATTY 1 cut(s) 807
XceI RCATGY 1 cut(s) 24
XhoI CTCGAG 2 cut(s) 562, 621
XmaJI CCTAGG 1 cut(s) 464
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 3 cut(s) 243, 465, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.