Prupe.4G191500_v2.0.a1

Protein of unknown function (DUF1223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0013222
Physical Location & Seq
Forward (+)
11513082 .. 11515147
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G191500.1

Sequence Viewer

Length: 819 bp
ATGGCACGTGGTCTCTTCGCCTGCTTTGGATCAAAAGGGGTCGTCGAGGGTACGCCCCGCGAAAAGCACGCGGGAGCGACGGCGGACATTTCTGCCGAGGAGCAGCGGCGGAGCGGGCCAGTTCTGGTGGAGCTGTTCTCGTCGCAGGGCTGCACCACCTCGCCGGTGGCCGACCTGCTGGTGTCGAGGCTTGGGAGGGGGGATTTTAATGAGATGGACCTGCCCCCAGTTGTGGTCCTGGCCTACCACGTGGACTACTGGGACTACATGGGCTGGAAGGACCCATTTGGGTCCAGCCAGTGGACTGTACGGCAGAAGGCCTATGTGGAGGCCTTGGGTCTGGACACCATGTTCACCCCCCAGATTGTGCTTCAAGGTAGGGACCAGTGTGTTGGCAATGATGAGAGTGCCTTGCTGTCGTCGATCAGGGCTGCACCTCGATACACTGCACCGACGTTCCAGGCAACTTTCGAAAGGCCCTCCCCAGACTCCTTGCAAGTATCTCTAACAGGATCCTTAAGGTCAAAGGTGGATAGTTATGGTGCCAATGTGATGGTGGCGCTCTATGAAAACGGGCTGATCACAGACTGCCCCAAGGGAGAGAACCAAGGACGTGTCCTGTCCAGTGACTTCGTTGTCCGAAGACTTGAAAAGCTCTCTACTGTCAAAGACGTCAATGCCAAGAAGACCATTTCGGGGACTCTCAACTTCCCCTTGTGGGAAGGCTTCAATCCCTCCAAGTGTGGTTTGGTCCTCTTTGTTCAAAACCCCTCCCATCAAATTTTTGGTTCACAGAACTTTCAGCTGCCGGATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1223

Protein Analysis

273

Amino Acids

29.81

Weight (kDa)

5.69

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27350 AT5G54240
fragaria_vesca FvH4_3g21110
malus_domestica MD03G1217700.v1.1 MD11G1235500.v1.1
prunus_persica Prupe.4G191500_v2.0.a1
pyrus_communis pycom03g16730 pycom1049g00020
rosa_chinensis RchiOBHm_Chr5g0036121
rosa_laevigata RLG00000033672
rosa_multiflora Rmu_sc0000382.1_g000011
rosa_roxburghii Rroxscaffold_1G00044620
rosa_rugosa Rorug05G0152700
rosa_samantha Rh5AG247200 Rh5BG247600 Rh5CG278500 Rh5DG255900
rosa_wichuraiana Rw5G022670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 635
AatII GACGTC 1 cut(s) 675
Acc36I ACCTGC 2 cut(s) 183, 228
AccB1I GGYRCC 1 cut(s) 542
AccB7I CCANNNNNTGG 1 cut(s) 300
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 2 cut(s) 60, 71
AciI CCGC 6 cut(s) 58, 71, 83, 106, 109, 114
AclWI GGATC 3 cut(s) 37, 507, 520
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 1 cut(s) 780
AcvI CACGTG 2 cut(s) 8, 250
AcyI GRCGYC 1 cut(s) 672
AfaI GTAC 2 cut(s) 52, 309
AfiI CCNNNNNNNGG 4 cut(s) 232, 300, 696, 718
AflII CTTAAG 1 cut(s) 517
AflIII ACRYGT 1 cut(s) 613
AgsI TTSAA 4 cut(s) 374, 650, 730, 764
AjiI CACGTC 1 cut(s) 614
AjnI CCWGG 2 cut(s) 237, 459
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 3 cut(s) 133, 655, 805
AluI AGCT 3 cut(s) 133, 655, 805
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 3 cut(s) 37, 507, 520
AoxI GGCC 6 cut(s) 116, 168, 240, 318, 330, 476
ApeKI GCWGC 4 cut(s) 103, 150, 431, 805
ApoI RAATTY 1 cut(s) 780
AspLEI GCGC 1 cut(s) 562
AspS9I GGNCC 8 cut(s) 116, 217, 235, 280, 291, 382, 477, 751
AsuHPI GGTGA 1 cut(s) 346
AsuII TTCGAA 1 cut(s) 471
AvaII GGWCC 6 cut(s) 217, 235, 280, 291, 382, 751
BamHI GGATCC 1 cut(s) 512
BanI GGYRCC 1 cut(s) 542
BbrPI CACGTG 2 cut(s) 8, 250
BbsI GAAGAC 2 cut(s) 649, 692
BbvI GCAGC 4 cut(s) 115, 137, 418, 792
BccI CCATC 3 cut(s) 208, 547, 783
BceAI ACGGC 2 cut(s) 96, 326
BciT130I CCWGG 2 cut(s) 239, 461
BclI TGATCA 1 cut(s) 579
BcoDI GTCTC 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 563
BfrI CTTAAG 1 cut(s) 517
BfuAI ACCTGC 2 cut(s) 183, 228
BisI GCNGC 5 cut(s) 104, 107, 151, 432, 806
BlsI GCNGC 5 cut(s) 105, 108, 152, 433, 807
Bme1390I CCNGG 2 cut(s) 239, 461
Bme18I GGWCC 6 cut(s) 217, 235, 280, 291, 382, 751
BmgBI CACGTC 1 cut(s) 614
BmgT120I GGNCC 8 cut(s) 116, 217, 235, 280, 291, 382, 477, 751
BmiI GGNNCC 5 cut(s) 282, 292, 383, 514, 544
BmrFI CCNGG 2 cut(s) 239, 461
BmrI ACTGGG 2 cut(s) 221, 268
BmuI ACTGGG 2 cut(s) 221, 268
BpiI GAAGAC 2 cut(s) 649, 692
BplI GAGNNNNNCTC 2 cut(s) 122, 154
Bpu14I TTCGAA 1 cut(s) 471
BsaAI YACGTR 2 cut(s) 8, 250
BsaHI GRCGYC 1 cut(s) 672
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 96, 333, 594, 607
Bsc4I CCNNNNNNNGG 4 cut(s) 232, 300, 696, 718
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 6 cut(s) 119, 227, 263, 298, 385, 624
Bse3DI GCAATG 1 cut(s) 403
BseBI CCWGG 2 cut(s) 239, 461
BseDI CCNNGG 4 cut(s) 96, 333, 594, 607
BseLI CCNNNNNNNGG 4 cut(s) 232, 300, 696, 718
BseMI GCAATG 1 cut(s) 403
BseNI ACTGG 6 cut(s) 119, 227, 263, 298, 385, 624
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 4 cut(s) 115, 137, 418, 792
BsgI GTGCAG 3 cut(s) 136, 417, 432
Bsh1236I CGCG 2 cut(s) 60, 71
BshFI GGCC 6 cut(s) 118, 170, 242, 320, 332, 478
BshNI GGYRCC 1 cut(s) 542
BsiSI CCGG 2 cut(s) 164, 809
BslFI GGGAC 3 cut(s) 275, 395, 712
BslI CCNNNNNNNGG 4 cut(s) 232, 300, 696, 718
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 3 cut(s) 275, 395, 712
BsnI GGCC 6 cut(s) 118, 170, 242, 320, 332, 478
Bso31I GGTCTC 1 cut(s) 17
Bsp119I TTCGAA 1 cut(s) 471
Bsp143I GATC 4 cut(s) 29, 423, 512, 579
BspACI CCGC 6 cut(s) 58, 71, 83, 106, 109, 114
BspANI GGCC 6 cut(s) 118, 170, 242, 320, 332, 478
BspFNI CGCG 2 cut(s) 60, 71
BspLI GGNNCC 5 cut(s) 282, 292, 383, 514, 544
BspMI ACCTGC 2 cut(s) 183, 228
BspPI GGATC 3 cut(s) 37, 507, 520
BspT104I TTCGAA 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 542
BspTI CTTAAG 1 cut(s) 517
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 403
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 6 cut(s) 119, 227, 263, 298, 385, 624
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 4 cut(s) 96, 333, 594, 607
BssMI GATC 4 cut(s) 29, 423, 512, 579
BssNI GRCGYC 1 cut(s) 672
BssT1I CCWWGG 3 cut(s) 333, 594, 607
Bst2UI CCWGG 2 cut(s) 239, 461
Bst4CI ACNGT 2 cut(s) 307, 664
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 672
BstAFI CTTAAG 1 cut(s) 517
BstBAI YACGTR 2 cut(s) 8, 250
BstBI TTCGAA 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 22, 69, 116
BstFNI CGCG 2 cut(s) 60, 71
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 1 cut(s) 562
BstKTI GATC 4 cut(s) 32, 426, 515, 582
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 4 cut(s) 29, 423, 512, 579
BstMWI GCNNNNNNNGC 1 cut(s) 115
BstNI CCWGG 2 cut(s) 239, 461
BstSCI CCNGG 2 cut(s) 237, 459
BstUI CGCG 2 cut(s) 60, 71
BstV1I GCAGC 4 cut(s) 115, 137, 418, 792
BstV2I GAAGAC 2 cut(s) 649, 692
BstX2I RGATCY 1 cut(s) 512
BstXI CCANNNNNNTGG 2 cut(s) 392, 553
BstYI RGATCY 1 cut(s) 512
BsuRI GGCC 6 cut(s) 118, 170, 242, 320, 332, 478
BtrI CACGTC 1 cut(s) 614
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 4 cut(s) 305, 392, 444, 631
BveI ACCTGC 2 cut(s) 183, 228
Cac8I GCNNGC 3 cut(s) 22, 69, 116
CfoI GCGC 1 cut(s) 562
Cfr10I RCCGGY 1 cut(s) 163
Cfr13I GGNCC 8 cut(s) 116, 217, 235, 280, 291, 382, 477, 751
Csp6I GTAC 2 cut(s) 51, 308
CviAII CATG 2 cut(s) 268, 349
CviQI GTAC 2 cut(s) 51, 308
DpnI GATC 4 cut(s) 31, 425, 514, 581
DpnII GATC 4 cut(s) 29, 423, 512, 579
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 2 cut(s) 98, 124
Eco130I CCWWGG 3 cut(s) 333, 594, 607
Eco147I AGGCCT 2 cut(s) 320, 332
Eco31I GGTCTC 1 cut(s) 17
Eco47I GGWCC 6 cut(s) 217, 235, 280, 291, 382, 751
Eco72I CACGTG 2 cut(s) 8, 250
EcoO109I RGGNCCY 2 cut(s) 280, 477
EcoRII CCWGG 2 cut(s) 237, 459
EcoT14I CCWWGG 3 cut(s) 333, 594, 607
ErhI CCWWGG 3 cut(s) 333, 594, 607
FaeI CATG 2 cut(s) 271, 352
FaiI YATR 5 cut(s) 269, 324, 350, 540, 567
FaqI GGGAC 3 cut(s) 275, 395, 712
FatI CATG 2 cut(s) 267, 348
FauI CCCGC 3 cut(s) 64, 65, 107
FbaI TGATCA 1 cut(s) 579
Fnu4HI GCNGC 5 cut(s) 104, 107, 151, 432, 806
Fsp4HI GCNGC 5 cut(s) 104, 107, 151, 432, 806
GlaI GCGC 1 cut(s) 561
GluI GCNGC 5 cut(s) 104, 107, 151, 432, 806
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 6 cut(s) 118, 170, 242, 320, 332, 478
HapII CCGG 2 cut(s) 164, 809
HhaI GCGC 1 cut(s) 562
Hin1I GRCGYC 1 cut(s) 672
Hin1II CATG 2 cut(s) 271, 352
Hin6I GCGC 1 cut(s) 560
HinP1I GCGC 1 cut(s) 560
HinfI GANTC 2 cut(s) 488, 700
HpaII CCGG 2 cut(s) 164, 809
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 4 cut(s) 253, 303, 354, 791
Hpy188I TCNGA 1 cut(s) 641
Hpy188III TCNNGA 1 cut(s) 341
Hpy8I GTNNAC 4 cut(s) 253, 303, 354, 791
Hpy99I CGWCG 5 cut(s) 47, 82, 145, 424, 457
HpyAV CCTTC 3 cut(s) 271, 310, 716
HpyCH4III ACNGT 2 cut(s) 307, 664
HpyCH4IV ACGT 5 cut(s) 7, 249, 455, 613, 672
HpyCH4V TGCA 4 cut(s) 153, 434, 449, 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 115
HpySE526I ACGT 5 cut(s) 7, 249, 455, 613, 672
Hsp92I GRCGYC 1 cut(s) 672
Hsp92II CATG 2 cut(s) 271, 352
HspAI GCGC 1 cut(s) 560
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 4 cut(s) 29, 423, 512, 579
LmnI GCTCC 4 cut(s) 74, 100, 111, 130
Lsp1109I GCAGC 4 cut(s) 115, 137, 418, 792
MaeII ACGT 5 cut(s) 7, 249, 455, 613, 672
MaeIII GTNAC 1 cut(s) 626
MalI GATC 4 cut(s) 31, 425, 514, 581
MbiI CCGCTC 1 cut(s) 114
MboI GATC 4 cut(s) 29, 423, 512, 579
MboII GAAGA 3 cut(s) 7, 654, 697
MflI RGATCY 1 cut(s) 512
MluCI AATT 2 cut(s) 780, 814
MlyI GAGTC 2 cut(s) 482, 694
MseI TTAA 2 cut(s) 207, 518
MspA1I CMGCKG 2 cut(s) 106, 805
MspCI CTTAAG 1 cut(s) 517
MspI CCGG 2 cut(s) 164, 809
MspR9I CCNGG 2 cut(s) 239, 461
MvaI CCWGG 2 cut(s) 239, 461
MvnI CGCG 2 cut(s) 60, 71
MwoI GCNNNNNNNGC 1 cut(s) 115
NdeII GATC 4 cut(s) 29, 423, 512, 579
NlaIII CATG 2 cut(s) 271, 352
NlaIV GGNNCC 5 cut(s) 282, 292, 383, 514, 544
NmeAIII GCCGAG 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 626
NspV TTCGAA 1 cut(s) 471
PceI AGGCCT 2 cut(s) 320, 332
PflMI CCANNNNNTGG 1 cut(s) 300
PkrI GCNGC 5 cut(s) 105, 108, 152, 433, 807
PleI GAGTC 2 cut(s) 482, 694
PmaCI CACGTG 2 cut(s) 8, 250
PmlI CACGTG 2 cut(s) 8, 250
PpsI GAGTC 2 cut(s) 482, 694
Ppu21I YACGTR 2 cut(s) 8, 250
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 237, 459
PspCI CACGTG 2 cut(s) 8, 250
PspGI CCWGG 2 cut(s) 237, 459
PspN4I GGNNCC 5 cut(s) 282, 292, 383, 514, 544
PspPI GGNCC 8 cut(s) 116, 217, 235, 280, 291, 382, 477, 751
PspPPI RGGWCCY 1 cut(s) 280
PsuI RGATCY 1 cut(s) 512
PvuII CAGCTG 1 cut(s) 805
RsaI GTAC 2 cut(s) 52, 309
RsaNI GTAC 2 cut(s) 51, 308
SaqAI TTAA 2 cut(s) 207, 518
SatI GCNGC 5 cut(s) 104, 107, 151, 432, 806
Sau3AI GATC 4 cut(s) 29, 423, 512, 579
Sau96I GGNCC 8 cut(s) 116, 217, 235, 280, 291, 382, 477, 751
SchI GAGTC 2 cut(s) 482, 694
ScrFI CCNGG 2 cut(s) 239, 461
SfuI TTCGAA 1 cut(s) 471
SgrAI CRCCGGYG 1 cut(s) 163
SinI GGWCC 6 cut(s) 217, 235, 280, 291, 382, 751
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
Sse9I AATT 2 cut(s) 780, 814
SseBI AGGCCT 2 cut(s) 320, 332
SsiI CCGC 6 cut(s) 58, 71, 83, 106, 109, 114
StuI AGGCCT 2 cut(s) 320, 332
StyD4I CCNGG 2 cut(s) 237, 459
StyI CCWWGG 3 cut(s) 333, 594, 607
TaaI ACNGT 2 cut(s) 307, 664
TaiI ACGT 5 cut(s) 10, 252, 458, 616, 675
TaqI TCGA 5 cut(s) 45, 185, 422, 439, 471
TasI AATT 2 cut(s) 780, 814
TauI GCSGC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 207, 518
Tru9I TTAA 2 cut(s) 207, 518
TscAI CASTG 4 cut(s) 305, 392, 451, 631
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 4 cut(s) 103, 150, 431, 805
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 1 cut(s) 582
TspRI CASTG 4 cut(s) 305, 392, 451, 631
Van91I CCANNNNNTGG 1 cut(s) 300
Vha464I CTTAAG 1 cut(s) 517
VpaK11BI GGWCC 6 cut(s) 217, 235, 280, 291, 382, 751
XapI RAATTY 1 cut(s) 780
XcmI CCANNNNNNNNNTGG 4 cut(s) 163, 553, 745, 782
ZraI GACGTC 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.