AT4G28020

Uncharacterised protein family UPF0066

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002784
Physical Location & Seq
Reverse (-)
13933232 .. 13935479
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28020.4

Sequence Viewer

Length: 1056 bp
ATGGCTACATCAGGACGCTCCCACGGCTCTACCACGGCGGCTTTAGCCTTTACACTCGCCGTAGTATCTGTATCCGCTGCCTTCTCGCTGTATAGATGGCGAAGACGAGAAGAAGATTTGGAGAATAGGATCAAGGAATTTGAGAAGTCTTTGGGATCTACCTTGGAAAAAAGTGCTTCTGAGAGAAAAGGTCGAGTCAAAGCTCAGCAGGCTTTACGGGAGGCGTTAGCACAGACTGAGTCACATGATTTACACAGCACAATATACCCGATGCGACCTATTGGCACTATCCAGTCTTGCTTCTCTACTAGGAACGGGACACCGAGGCAGCCATTGCTTGTTTCTCTTGCGAGGGCATGTTTGATCTTTGATCCGGCTTTGGTTCCTCCTGCGTCTCTTGAGGGTCTTGAAGAGTATTCTCATTGCTGGATACTCTATGTGTTTCATCTCAATACTGATATCGAGAAGCTATGGAGAAAACCATCTCAGTCGAAGCTCAAGGCAAAGGTGAGAGTGCCACGGCTAAATGGGGAACGGAAGGGAGTCTTTGCTACGCGGTCCCCTCATCGACCTTGTCCCATTGGCCTCACCGTTGCTAAGGTGGAGGAAATCCAAAAGGATAAGGTTCTCCTCTCTGGTGTCGATCTGGTGGATGGGACTCCGGTGCTCGACATCAAACCATATTTACCATACTCGGACAGTATTCAAGGAGCCTCAGTGCCAAACTGGGTAAAGGAGGACTGCTCATTGGCTGTGGCTTCTGTGACTTTCTCAGATACCTTCTCTTCATCCATCACCAGCTGCTGGAAACTGATAGAAAAGAAGTCTCTATATAGTTCAGCAGATGAGTTTAGGAGCTTGATCACGCAGGTCCTGTCATGGGACATACGATCAATGTCGCAACGGAACAAGCCACAAGATACATTGGATGAGGAAATTGTTTATCACTTGGTTCTGGAGGGACTAGACGTGTCTTACATGATAGATAATGAAAGTAATATTCTCGTTCAGGATGTTTCTCTTCCCAAAAACCTGCAAGATGTTGCAGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0066

Protein Analysis

351

Amino Acids

39.17

Weight (kDa)

7.62

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmO_N PF01980 106 - 233 4.3e-40 tRNA-methyltransferase O N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 859, 1041
AccB7I CCANNNNNTGG 1 cut(s) 804
AccII CGCG 1 cut(s) 556
AciI CCGC 3 cut(s) 38, 75, 556
AclWI GGATC 3 cut(s) 137, 163, 365
AcsI RAATTY 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 804, 880
AflIII ACRYGT 1 cut(s) 969
AgsI TTSAA 2 cut(s) 410, 707
AjiI CACGTC 1 cut(s) 970
AluBI AGCT 6 cut(s) 203, 469, 496, 801, 858, 1053
AluI AGCT 6 cut(s) 203, 469, 496, 801, 858, 1053
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 2 cut(s) 399, 831
AlwI GGATC 3 cut(s) 137, 163, 365
AlwNI CAGNNNCTG 2 cut(s) 804, 874
AoxI GGCC 1 cut(s) 583
ApeKI GCWGC 3 cut(s) 77, 328, 801
ApoI RAATTY 1 cut(s) 137
AspS9I GGNCC 2 cut(s) 558, 871
AsuHPI GGTGA 3 cut(s) 520, 580, 787
AvaII GGWCC 2 cut(s) 558, 871
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 3 cut(s) 64, 340, 788
BccI CCATC 4 cut(s) 90, 490, 647, 800
BceAI ACGGC 4 cut(s) 40, 44, 51, 536
BciVI GTATCC 2 cut(s) 82, 423
BclI TGATCA 1 cut(s) 861
BcoDI GTCTC 2 cut(s) 399, 831
BfaI CTAG 2 cut(s) 309, 965
BfuAI ACCTGC 2 cut(s) 859, 1041
BfuI GTATCC 2 cut(s) 82, 423
BisI GCNGC 4 cut(s) 39, 78, 329, 802
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 4 cut(s) 40, 79, 330, 803
Bme18I GGWCC 2 cut(s) 558, 871
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 2 cut(s) 558, 871
BmiI GGNNCC 3 cut(s) 384, 560, 712
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 1 cut(s) 261
BmuI ACTGGG 1 cut(s) 736
BpiI GAAGAC 1 cut(s) 109
BplI GAGNNNNNCTC 2 cut(s) 728, 760
BpmI CTGGAG 1 cut(s) 977
Bpu10I CCTNAGC 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 2 cut(s) 419, 482
BsaJI CCNNGG 5 cut(s) 22, 33, 162, 323, 518
BsaWI WCCGGW 1 cut(s) 661
BsaXI ACNNNNNCTCC 2 cut(s) 702, 732
Bsc4I CCNNNNNNNGG 2 cut(s) 804, 880
Bse1I ACTGG 2 cut(s) 292, 731
Bse3DI GCAATG 2 cut(s) 332, 421
BseDI CCNNGG 5 cut(s) 22, 33, 162, 323, 518
BseGI GGATG 4 cut(s) 658, 788, 934, 1018
BseLI CCNNNNNNNGG 2 cut(s) 804, 880
BseMI GCAATG 2 cut(s) 332, 421
BseMII CTCAG 6 cut(s) 171, 218, 228, 500, 729, 786
BseNI ACTGG 2 cut(s) 292, 731
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 3 cut(s) 64, 340, 788
Bsh1236I CGCG 1 cut(s) 556
BshFI GGCC 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 669
BsiSI CCGG 2 cut(s) 374, 662
BslFI GGGAC 6 cut(s) 331, 544, 561, 670, 896, 975
BslI CCNNNNNNNGG 2 cut(s) 804, 880
BsmAI GTCTC 2 cut(s) 399, 831
BsmBI CGTCTC 1 cut(s) 399
BsmFI GGGAC 6 cut(s) 331, 544, 561, 670, 896, 975
BsnI GGCC 1 cut(s) 585
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 7 cut(s) 129, 155, 363, 370, 643, 861, 890
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 3 cut(s) 38, 75, 556
BspANI GGCC 1 cut(s) 585
BspCNI CTCAG 6 cut(s) 172, 217, 229, 499, 728, 785
BspFNI CGCG 1 cut(s) 556
BspLI GGNNCC 3 cut(s) 384, 560, 712
BspMI ACCTGC 2 cut(s) 859, 1041
BspPI GGATC 3 cut(s) 137, 163, 365
BsrDI GCAATG 2 cut(s) 332, 421
BsrI ACTGG 2 cut(s) 292, 731
BssECI CCNNGG 5 cut(s) 22, 33, 162, 323, 518
BssMI GATC 7 cut(s) 129, 155, 363, 370, 643, 861, 890
BssT1I CCWWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 592, 701
Bst6I CTCTTC 3 cut(s) 405, 790, 1026
BstAPI GCANNNNNTGC 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 7 cut(s) 180, 204, 237, 486, 597, 715, 772
BstDSI CCRYGG 3 cut(s) 22, 33, 518
BstF5I GGATG 4 cut(s) 658, 788, 934, 1018
BstFNI CGCG 1 cut(s) 556
BstKTI GATC 7 cut(s) 132, 158, 366, 373, 646, 864, 893
BstMAI GTCTC 2 cut(s) 399, 831
BstMBI GATC 7 cut(s) 129, 155, 363, 370, 643, 861, 890
BstMWI GCNNNNNNNGC 4 cut(s) 24, 44, 209, 334
BstNSI RCATGY 1 cut(s) 360
BstUI CGCG 1 cut(s) 556
BstV1I GCAGC 3 cut(s) 64, 340, 788
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
BsuI GTATCC 2 cut(s) 82, 423
BsuRI GGCC 1 cut(s) 585
BtgI CCRYGG 3 cut(s) 22, 33, 518
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 4 cut(s) 658, 788, 934, 1018
BtsIMutI CAGTG 1 cut(s) 723
BveI ACCTGC 2 cut(s) 859, 1041
Cac8I GCNNGC 1 cut(s) 210
CaiI CAGNNNCTG 2 cut(s) 804, 874
Cfr13I GGNCC 2 cut(s) 558, 871
CseI GACGC 2 cut(s) 24, 381
CviAII CATG 4 cut(s) 245, 357, 879, 979
DdeI CTNAG 7 cut(s) 180, 204, 237, 486, 597, 715, 772
DpnI GATC 7 cut(s) 131, 157, 365, 372, 645, 863, 892
DpnII GATC 7 cut(s) 129, 155, 363, 370, 643, 861, 890
Eam1104I CTCTTC 3 cut(s) 405, 790, 1026
EarI CTCTTC 3 cut(s) 405, 790, 1026
Eco130I CCWWGG 1 cut(s) 162
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 2 cut(s) 558, 871
EcoO109I RGGNCCY 1 cut(s) 871
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 4 cut(s) 248, 360, 882, 982
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FaqI GGGAC 6 cut(s) 331, 544, 561, 670, 896, 975
FatI CATG 4 cut(s) 244, 356, 878, 978
FbaI TGATCA 1 cut(s) 861
Fnu4HI GCNGC 4 cut(s) 39, 78, 329, 802
FokI GGATG 4 cut(s) 665, 775, 941, 1025
Fsp4HI GCNGC 4 cut(s) 39, 78, 329, 802
FspBI CTAG 2 cut(s) 309, 965
GluI GCNGC 4 cut(s) 39, 78, 329, 802
GsuI CTGGAG 1 cut(s) 977
HaeIII GGCC 1 cut(s) 585
HapII CCGG 2 cut(s) 374, 662
HgaI GACGC 2 cut(s) 24, 381
Hin1II CATG 4 cut(s) 248, 360, 882, 982
HinfI GANTC 4 cut(s) 195, 239, 543, 658
HpaII CCGG 2 cut(s) 374, 662
HphI GGTGA 3 cut(s) 520, 580, 787
Hpy188I TCNGA 3 cut(s) 181, 697, 775
Hpy188III TCNNGA 6 cut(s) 12, 398, 407, 463, 956, 1010
HpyAV CCTTC 3 cut(s) 91, 532, 790
HpyCH4III ACNGT 2 cut(s) 592, 701
HpyCH4IV ACGT 1 cut(s) 969
HpyCH4V TGCA 2 cut(s) 1036, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 44, 209, 334
HpyF3I CTNAG 7 cut(s) 180, 204, 237, 486, 597, 715, 772
HpySE526I ACGT 1 cut(s) 969
Hsp92II CATG 4 cut(s) 248, 360, 882, 982
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 7 cut(s) 129, 155, 363, 370, 643, 861, 890
LmnI GCTCC 3 cut(s) 23, 710, 855
Lsp1109I GCAGC 3 cut(s) 64, 340, 788
LweI GCATC 1 cut(s) 261
MaeI CTAG 2 cut(s) 309, 965
MaeII ACGT 1 cut(s) 969
MaeIII GTNAC 2 cut(s) 240, 763
MalI GATC 7 cut(s) 131, 157, 365, 372, 645, 863, 892
MboI GATC 7 cut(s) 129, 155, 363, 370, 643, 861, 890
MboII GAAGA 6 cut(s) 114, 122, 125, 422, 777, 1013
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 2 cut(s) 137, 936
MlyI GAGTC 4 cut(s) 204, 248, 552, 652
MspA1I CMGCKG 2 cut(s) 77, 801
MspI CCGG 2 cut(s) 374, 662
MvnI CGCG 1 cut(s) 556
MwoI GCNNNNNNNGC 4 cut(s) 24, 44, 209, 334
NdeII GATC 7 cut(s) 129, 155, 363, 370, 643, 861, 890
NlaIII CATG 4 cut(s) 248, 360, 882, 982
NlaIV GGNNCC 3 cut(s) 384, 560, 712
NmuCI GTSAC 2 cut(s) 240, 763
NspI RCATGY 1 cut(s) 360
PflFI GACNNNGTC 2 cut(s) 238, 573
PflMI CCANNNNNTGG 1 cut(s) 804
PkrI GCNGC 4 cut(s) 40, 79, 330, 803
PleI GAGTC 4 cut(s) 203, 247, 551, 652
PpsI GAGTC 4 cut(s) 203, 247, 551, 652
PpuMI RGGWCCY 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 871
PspN4I GGNNCC 3 cut(s) 384, 560, 712
PspPI GGNCC 2 cut(s) 558, 871
PspPPI RGGWCCY 1 cut(s) 871
PstNI CAGNNNCTG 2 cut(s) 804, 874
PsuI RGATCY 1 cut(s) 155
PsyI GACNNNGTC 2 cut(s) 238, 573
PvuII CAGCTG 1 cut(s) 801
SatI GCNGC 4 cut(s) 39, 78, 329, 802
Sau3AI GATC 7 cut(s) 129, 155, 363, 370, 643, 861, 890
Sau96I GGNCC 2 cut(s) 558, 871
SchI GAGTC 4 cut(s) 204, 248, 552, 652
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 1 cut(s) 261
SinI GGWCC 2 cut(s) 558, 871
SmlI CTYRAG 2 cut(s) 398, 497
SmoI CTYRAG 2 cut(s) 398, 497
Sse9I AATT 2 cut(s) 137, 936
SsiI CCGC 3 cut(s) 38, 75, 556
SspI AATATT 1 cut(s) 1000
SspMI CTAG 2 cut(s) 309, 965
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 592, 701
TaiI ACGT 1 cut(s) 972
TaqI TCGA 6 cut(s) 193, 462, 491, 568, 642, 669
TasI AATT 2 cut(s) 137, 936
TauI GCSGC 1 cut(s) 41
TscAI CASTG 1 cut(s) 723
TseFI GTSAC 2 cut(s) 240, 763
TseI GCWGC 3 cut(s) 77, 328, 801
Tsp45I GTSAC 2 cut(s) 240, 763
TspDTI ATGAA 3 cut(s) 434, 777, 1005
TspGWI ACGGA 2 cut(s) 550, 919
TspRI CASTG 1 cut(s) 723
Tth111I GACNNNGTC 2 cut(s) 238, 573
Van91I CCANNNNNTGG 1 cut(s) 804
VpaK11BI GGWCC 2 cut(s) 558, 871
XapI RAATTY 1 cut(s) 137
XceI RCATGY 1 cut(s) 360
XspI CTAG 2 cut(s) 309, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.