Rh4AG123400

Uncharacterised protein family UPF0066

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002784
Physical Location & Seq
Reverse (-)
26618095 .. 26626641
8547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG123400.1

Sequence Viewer

Length: 867 bp
ATGGGTTTCGCTACAGAATCCAAATGGTTAAGAGCCACCATAGCCGTCGCACTCGCAGCTCTCTCCGCCTCCACTGTTCTTTCTCTGTATTTCTTGAAAAGAAAGTCCAAGGATCTGGACTCCAAGATTAGGGAGCTCGAGAAATCCCTCAAGGACTCCTTAAAGCACTGTGCTTCCGAAAGGCAAGGTCGAATTCGGGCTCAGCAGGCTTTGAGGAAATCGCTGACGGAACCAAAGGTTGATGATTTGGAGCTGACTTCTTACCCCATGGCACCTATTGGTGTCATCCAGTCATGCTTCTCCACCAGGAATGGGACACCCAGGCAACCATTAATTGTACCTCTTTCCAGGGCATGCTTGATCTTTAATTCAGCCCGTGTTCCTCCAGCTTCGCTTGAGGGTCTTGGAGACTACACGCACTGTTGGGTTATCTATGTTTTCCATCTAAATACAAATCTAGAGAAGCTTTGGAAGGATCCAGCAAAATCGAAGTTTAAGGCCAAGGTGAGAGTGCCAAGACTAAAAGGTGAACGCATGGGAGTCTTTGCCACCCGATCACCTCACAGGCCATGCCCCATTGGGCTCACTGTTGCAAAGGTGGAGGCTGTAAAAGGGAATATACTTCTACTCTCTGGTGTGGATTTGGTTGATGGGACGCCTGTACTTGATGTCAAACCGTATCTTCCTTACTGTGACAGCATCCAAGAAGCAGGAGTGCCAAAGTGGTTAACGGTATTATTTCCATGGTCTTTCAGGAAGTTGTTAATACCTCTATTTCTCCATGATATAAAAGATTTAACTTTTAATGATAAGAGGATCATCTTCCTTTATAATCCAAGAACTTTGGGCTTTCAAGGTGGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0066

Protein Analysis

288

Amino Acids

32.25

Weight (kDa)

9.78

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmO_N PF01980 105 - 232 1.5e-38 tRNA-methyltransferase O N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 831
AccB1I GGYRCC 1 cut(s) 271
AciI CCGC 1 cut(s) 66
AclWI GGATC 4 cut(s) 120, 470, 483, 824
AcsI RAATTY 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 656
AfaI GTAC 2 cut(s) 339, 663
AfiI CCNNNNNNNGG 2 cut(s) 129, 312
AgsI TTSAA 2 cut(s) 97, 854
AjnI CCWGG 3 cut(s) 305, 320, 347
AluBI AGCT 5 cut(s) 59, 136, 253, 389, 466
AluI AGCT 5 cut(s) 59, 136, 253, 389, 466
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 4 cut(s) 120, 470, 483, 824
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 2 cut(s) 498, 566
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 1 cut(s) 192
AseI ATTAAT 1 cut(s) 332
AsuHPI GGTGA 3 cut(s) 517, 539, 549
AvaI CYCGRG 1 cut(s) 137
BamHI GGATCC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 3 cut(s) 138, 202, 585
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 68
BccI CCATC 2 cut(s) 450, 644
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 307, 322, 349
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 12
BisI GCNGC 1 cut(s) 57
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 3 cut(s) 307, 322, 349
BmeT110I CYCGRG 1 cut(s) 137
BmiI GGNNCC 3 cut(s) 231, 273, 477
BmrFI CCNGG 3 cut(s) 307, 322, 349
BmsI GCATC 1 cut(s) 708
BpmI CTGGAG 1 cut(s) 369
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 134, 416
BsaHI GRCGYC 1 cut(s) 656
BsaJI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 743
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 312
Bse1I ACTGG 1 cut(s) 289
BseBI CCWGG 3 cut(s) 307, 322, 349
BseDI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 743
BseGI GGATG 2 cut(s) 285, 699
BseLI CCNNNNNNNGG 2 cut(s) 129, 312
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 289
BseXI GCAGC 1 cut(s) 68
BshFI GGCC 2 cut(s) 500, 568
BshNI GGYRCC 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 137
BslFI GGGAC 2 cut(s) 328, 667
BslI CCNNNNNNNGG 2 cut(s) 129, 312
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 2 cut(s) 328, 667
BsnI GGCC 2 cut(s) 500, 568
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 3 cut(s) 138, 202, 585
Bsp143I GATC 5 cut(s) 112, 360, 475, 554, 816
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 2 cut(s) 267, 743
BspACI CCGC 1 cut(s) 66
BspANI GGCC 2 cut(s) 500, 568
BspCNI CTCAG 1 cut(s) 214
BspLI GGNNCC 3 cut(s) 231, 273, 477
BspPI GGATC 4 cut(s) 120, 470, 483, 824
BspT107I GGYRCC 1 cut(s) 271
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 743
BssMI GATC 5 cut(s) 112, 360, 475, 554, 816
BssNI GRCGYC 1 cut(s) 656
BssT1I CCWWGG 4 cut(s) 108, 267, 501, 743
Bst2UI CCWGG 3 cut(s) 307, 322, 349
Bst4CI ACNGT 7 cut(s) 76, 170, 422, 589, 678, 692, 733
BstACI GRCGYC 1 cut(s) 656
BstC8I GCNNGC 2 cut(s) 207, 355
BstDEI CTNAG 1 cut(s) 201
BstDSI CCRYGG 2 cut(s) 267, 743
BstF5I GGATG 2 cut(s) 285, 699
BstKTI GATC 5 cut(s) 115, 363, 478, 557, 819
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 5 cut(s) 112, 360, 475, 554, 816
BstMWI GCNNNNNNNGC 4 cut(s) 41, 56, 65, 206
BstNI CCWGG 3 cut(s) 307, 322, 349
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 3 cut(s) 305, 320, 347
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 68
BstX2I RGATCY 2 cut(s) 112, 475
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 2 cut(s) 112, 475
BsuRI GGCC 2 cut(s) 500, 568
BtgI CCRYGG 2 cut(s) 267, 743
BtsCI GGATG 2 cut(s) 285, 699
BtsIMutI CAGTG 4 cut(s) 72, 166, 418, 585
Cac8I GCNNGC 2 cut(s) 207, 355
CseI GACGC 1 cut(s) 664
Csp6I GTAC 2 cut(s) 338, 662
CviAII CATG 7 cut(s) 268, 294, 354, 535, 570, 744, 782
CviQI GTAC 2 cut(s) 338, 662
DdeI CTNAG 1 cut(s) 201
DpnI GATC 5 cut(s) 114, 362, 477, 556, 818
DpnII GATC 5 cut(s) 112, 360, 475, 554, 816
EciI GGCGGA 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 4 cut(s) 108, 267, 501, 743
Eco24I GRGCYC 3 cut(s) 138, 202, 585
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 192
EcoRII CCWGG 3 cut(s) 305, 320, 347
EcoT14I CCWWGG 4 cut(s) 108, 267, 501, 743
EcoT38I GRGCYC 3 cut(s) 138, 202, 585
ErhI CCWWGG 4 cut(s) 108, 267, 501, 743
FaeI CATG 7 cut(s) 271, 297, 357, 538, 573, 747, 785
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 328, 667
FatI CATG 7 cut(s) 267, 293, 353, 534, 569, 743, 781
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 2 cut(s) 272, 686
FriOI GRGCYC 3 cut(s) 138, 202, 585
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 1 cut(s) 458
GluI GCNGC 1 cut(s) 57
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 2 cut(s) 500, 568
HgaI GACGC 1 cut(s) 664
Hin1I GRCGYC 1 cut(s) 656
Hin1II CATG 7 cut(s) 271, 297, 357, 538, 573, 747, 785
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 4 cut(s) 17, 119, 155, 540
HpaI GTTAAC 1 cut(s) 729
HphI GGTGA 3 cut(s) 517, 539, 549
Hpy166II GTNNAC 2 cut(s) 530, 729
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 5 cut(s) 94, 116, 139, 458, 754
Hpy8I GTNNAC 2 cut(s) 530, 729
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 7 cut(s) 76, 170, 422, 589, 678, 692, 733
HpyCH4V TGCA 1 cut(s) 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 56, 65, 206
HpyF3I CTNAG 1 cut(s) 201
Hsp92I GRCGYC 1 cut(s) 656
Hsp92II CATG 7 cut(s) 271, 297, 357, 538, 573, 747, 785
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 5 cut(s) 112, 360, 475, 554, 816
LmnI GCTCC 2 cut(s) 133, 250
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 1 cut(s) 708
MaeI CTAG 1 cut(s) 458
MaeIII GTNAC 1 cut(s) 692
MalI GATC 5 cut(s) 114, 362, 477, 556, 818
MboI GATC 5 cut(s) 112, 360, 475, 554, 816
MboII GAAGA 2 cut(s) 674, 814
MflI RGATCY 2 cut(s) 112, 475
MhlI GDGCHC 3 cut(s) 138, 202, 585
MluCI AATT 3 cut(s) 192, 333, 367
MlyI GAGTC 3 cut(s) 113, 149, 549
MseI TTAA 9 cut(s) 29, 161, 332, 366, 495, 728, 764, 797, 804
MspR9I CCNGG 3 cut(s) 307, 322, 349
MvaI CCWGG 3 cut(s) 307, 322, 349
MwoI GCNNNNNNNGC 4 cut(s) 41, 56, 65, 206
NcoI CCATGG 2 cut(s) 267, 743
NdeII GATC 5 cut(s) 112, 360, 475, 554, 816
NlaIII CATG 7 cut(s) 271, 297, 357, 538, 573, 747, 785
NlaIV GGNNCC 3 cut(s) 231, 273, 477
NmuCI GTSAC 1 cut(s) 692
NspI RCATGY 1 cut(s) 357
PaeI GCATGC 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 137
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 3 cut(s) 113, 149, 548
PpsI GAGTC 3 cut(s) 113, 149, 548
PshBI ATTAAT 1 cut(s) 332
PsiI TTATAA 1 cut(s) 831
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 305, 320, 347
PspGI CCWGG 3 cut(s) 305, 320, 347
PspN4I GGNNCC 3 cut(s) 231, 273, 477
PsuI RGATCY 2 cut(s) 112, 475
RsaI GTAC 2 cut(s) 339, 663
RsaNI GTAC 2 cut(s) 338, 662
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 9 cut(s) 29, 161, 332, 366, 495, 728, 764, 797, 804
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 5 cut(s) 112, 360, 475, 554, 816
SchI GAGTC 3 cut(s) 113, 149, 549
ScrFI CCNGG 3 cut(s) 307, 322, 349
SduI GDGCHC 3 cut(s) 138, 202, 585
SfaNI GCATC 1 cut(s) 708
SfcI CTRYAG 1 cut(s) 12
Sfr274I CTCGAG 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 137
SmlI CTYRAG 3 cut(s) 137, 149, 395
SmoI CTYRAG 3 cut(s) 137, 149, 395
SphI GCATGC 1 cut(s) 357
Sse9I AATT 3 cut(s) 192, 333, 367
SsiI CCGC 1 cut(s) 66
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 3 cut(s) 305, 320, 347
StyI CCWWGG 4 cut(s) 108, 267, 501, 743
TaaI ACNGT 7 cut(s) 76, 170, 422, 589, 678, 692, 733
TaqI TCGA 3 cut(s) 138, 190, 488
TasI AATT 3 cut(s) 192, 333, 367
TatI WGTACW 1 cut(s) 661
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 9 cut(s) 29, 161, 332, 366, 495, 728, 764, 797, 804
Tru9I TTAA 9 cut(s) 29, 161, 332, 366, 495, 728, 764, 797, 804
TscAI CASTG 4 cut(s) 79, 173, 425, 592
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 1 cut(s) 692
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 4 cut(s) 79, 173, 425, 592
VspI ATTAAT 1 cut(s) 332
XapI RAATTY 1 cut(s) 192
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 1 cut(s) 357
XhoI CTCGAG 1 cut(s) 137
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.