Rroxscaffold_5G00348650

Uncharacterised protein family UPF0066

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002784
Physical Location & Seq
Reverse (-)
20986678 .. 20995294
8617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348650.1

Sequence Viewer

Length: 1170 bp
ATGGGTTTCGCTACAGAATCCAAATGGTTAAGAGCCACCATAGCCGTCGCACTCGCAGCTCTCTCCGCCTCCACTGCCCTTTCTCTGTATTTCTTGAAAAGAAAATCCAAGGATCTGGACTCCAAGATTAGGGAGCTCGAGAAATCCCTCAAGGACTCCTTAAAGCATTGTGCTTCCGAAAGGCAAGGTCGAATTCGGGCTCAGCAGGCTTTGAGGAAATCGCTGACGGAACCAAAGGTTGATGATTTGGAGCTGACTTCTTACCCCATGGCACCTATTGGTGTCATCCAGTCATGCTTCTCCACCAGGAATGGGACACCCAGGCAACCATTAATTGTACCTCTTTCCAGGGCATGCTTGATCTTTAATTCAGCCCGTGTTCCTCCAGCTTCGCTTGAGGGTCTTGGAGACTACACGCACTGTTGGGTTATCTATGTTTTCCATCTAAATACAAATCTAGAGAAGCTTTGGAAGGATCCAGCAAAATCGAAGTTTAAGGCCAAGGTGAGAGTGCCAAGACTAAAAGGTGAACGCATGGGAGTCTTTGCCACCCGATCACCACACAGGCCATGCCCCATTGGGCTCACTGTTGCAAAGGTGGAGGCTGTAAAAGGGAATATACTTCTACTCTCTGGTGTGGATTTGGTTGATGGGACACCTGTACTTGATGTCAAACCGTATCTTCCTTACTGTGACAGCATCCAAGAAGCAGGAGTGCCAAAGTGGTTAACGGTAGACAGGTCATTTTCAGTGGCTTCTATTAGTTTCTCTGAGGGCTTCACTTCAACACTGGCTCAATGTTGGGCCATAACAGGTAAGAATTCACTGTACGCATCACCAGATGAGTTCCAAAACTTGTTAAAGCAGGTGCTGTCATGGGATATTCGATCAGTATCTCAGCGAACACGTCCTCATGAGTCCATCACATCCCAAAATGGTAACCACTCGAATGATCTCTCAGATGACTACCAAGACGAAGAAGCTTCTAGCCCCGGAAACGAGCAGCCTCCGCAATCATCTGGCGACATAATTTATCACCTAATTCTAGAAGGATTGGATGTTTGGTACAGGCTCTGTGATGGGAATGTTGTTGTTGAGAAAGTGACAGAAGCATCAACCGTCATTAAAAGTAATCAAAAGCGGTGTAATTACTCTATATGGAGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0066

Protein Analysis

389

Amino Acids

43.32

Weight (kDa)

8.83

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmO_N PF01980 105 - 232 3e-38 tRNA-methyltransferase O N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 856
Acc36I ACCTGC 1 cut(s) 856
AccB1I GGYRCC 1 cut(s) 271
AccI GTMKAC 1 cut(s) 735
AciI CCGC 3 cut(s) 66, 1010, 1141
AclWI GGATC 3 cut(s) 120, 470, 483
AcsI RAATTY 2 cut(s) 192, 820
AfaI GTAC 4 cut(s) 339, 663, 830, 1067
AfiI CCNNNNNNNGG 2 cut(s) 129, 312
AflIII ACRYGT 1 cut(s) 905
AgsI TTSAA 2 cut(s) 97, 786
AjiI CACGTC 1 cut(s) 908
AjnI CCWGG 3 cut(s) 305, 320, 347
AluBI AGCT 6 cut(s) 59, 136, 253, 389, 466, 983
AluI AGCT 6 cut(s) 59, 136, 253, 389, 466, 983
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 402, 1158
AlwI GGATC 3 cut(s) 120, 470, 483
AlwNI CAGNNNCTG 2 cut(s) 871, 1074
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 3 cut(s) 498, 566, 804
ApeKI GCWGC 2 cut(s) 56, 1003
ApoI RAATTY 2 cut(s) 192, 820
AseI ATTAAT 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 804
AsuC2I CCSGG 1 cut(s) 993
AsuHPI GGTGA 5 cut(s) 517, 539, 549, 828, 1028
AvaI CYCGRG 1 cut(s) 137
BamHI GGATCC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 3 cut(s) 138, 202, 585
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 68, 1015
BccI CCATC 4 cut(s) 450, 644, 929, 1073
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 307, 322, 349
BcnI CCSGG 1 cut(s) 993
BcoDI GTCTC 2 cut(s) 402, 1158
BfaI CTAG 3 cut(s) 458, 987, 1046
BfmI CTRYAG 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 856
BisI GCNGC 2 cut(s) 57, 1004
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 2 cut(s) 58, 1005
Bme1390I CCNGG 4 cut(s) 307, 322, 349, 993
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 908
BmgT120I GGNCC 1 cut(s) 804
BmiI GGNNCC 3 cut(s) 231, 273, 477
BmrFI CCNGG 4 cut(s) 307, 322, 349, 993
BmsI GCATC 3 cut(s) 708, 842, 1121
BpmI CTGGAG 1 cut(s) 369
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 134, 416
BpuMI CCSGG 1 cut(s) 993
BsaBI GATNNNNATC 1 cut(s) 892
BsaJI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 991
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 312
Bse1I ACTGG 2 cut(s) 289, 795
Bse8I GATNNNNATC 1 cut(s) 892
BseBI CCWGG 3 cut(s) 307, 322, 349
BseDI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 991
BseGI GGATG 4 cut(s) 285, 699, 926, 1063
BseJI GATNNNNATC 1 cut(s) 892
BseLI CCNNNNNNNGG 2 cut(s) 129, 312
BseMII CTCAG 4 cut(s) 215, 762, 911, 972
BseNI ACTGG 2 cut(s) 289, 795
BseXI GCAGC 2 cut(s) 68, 1015
BshFI GGCC 3 cut(s) 500, 568, 806
BshNI GGYRCC 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 993
BslFI GGGAC 2 cut(s) 328, 667
BslI CCNNNNNNNGG 2 cut(s) 129, 312
BsmAI GTCTC 2 cut(s) 402, 1158
BsmFI GGGAC 2 cut(s) 328, 667
BsnI GGCC 3 cut(s) 500, 568, 806
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 3 cut(s) 138, 202, 585
Bsp143I GATC 6 cut(s) 112, 360, 475, 554, 887, 952
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 3 cut(s) 66, 1010, 1141
BspANI GGCC 3 cut(s) 500, 568, 806
BspCNI CTCAG 4 cut(s) 214, 763, 910, 971
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 3 cut(s) 231, 273, 477
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 3 cut(s) 120, 470, 483
BspT107I GGYRCC 1 cut(s) 271
BsrI ACTGG 2 cut(s) 289, 795
BssECI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 991
BssMI GATC 6 cut(s) 112, 360, 475, 554, 887, 952
BssT1I CCWWGG 3 cut(s) 108, 267, 501
Bst2UI CCWGG 3 cut(s) 307, 322, 349
Bst4CI ACNGT 7 cut(s) 422, 589, 678, 692, 733, 828, 1120
BstC8I GCNNGC 2 cut(s) 207, 355
BstDEI CTNAG 4 cut(s) 201, 771, 897, 958
BstDSI CCRYGG 1 cut(s) 267
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 4 cut(s) 285, 699, 926, 1063
BstKTI GATC 6 cut(s) 115, 363, 478, 557, 890, 955
BstMAI GTCTC 2 cut(s) 402, 1158
BstMBI GATC 6 cut(s) 112, 360, 475, 554, 887, 952
BstMWI GCNNNNNNNGC 6 cut(s) 41, 56, 65, 74, 206, 1009
BstNI CCWGG 3 cut(s) 307, 322, 349
BstNSI RCATGY 1 cut(s) 357
BstPI GGTNACC 1 cut(s) 938
BstSCI CCNGG 4 cut(s) 305, 320, 347, 991
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 68, 1015
BstX2I RGATCY 2 cut(s) 112, 475
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 2 cut(s) 112, 475
BsuRI GGCC 3 cut(s) 500, 568, 806
BtgI CCRYGG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 908
BtsCI GGATG 4 cut(s) 285, 699, 926, 1063
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 6 cut(s) 72, 418, 585, 756, 788, 824
BveI ACCTGC 1 cut(s) 856
Cac8I GCNNGC 2 cut(s) 207, 355
CaiI CAGNNNCTG 2 cut(s) 871, 1074
CciI TCATGA 1 cut(s) 913
Cfr13I GGNCC 1 cut(s) 804
Csp6I GTAC 4 cut(s) 338, 662, 829, 1066
CviAII CATG 7 cut(s) 268, 294, 354, 535, 570, 876, 914
CviQI GTAC 4 cut(s) 338, 662, 829, 1066
DdeI CTNAG 4 cut(s) 201, 771, 897, 958
DpnI GATC 6 cut(s) 114, 362, 477, 556, 889, 954
DpnII GATC 6 cut(s) 112, 360, 475, 554, 887, 952
EciI GGCGGA 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 108, 267, 501
Eco24I GRGCYC 3 cut(s) 138, 202, 585
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 938
EcoICRI GAGCTC 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 938
EcoRI GAATTC 2 cut(s) 192, 820
EcoRII CCWGG 3 cut(s) 305, 320, 347
EcoT14I CCWWGG 3 cut(s) 108, 267, 501
EcoT38I GRGCYC 3 cut(s) 138, 202, 585
ErhI CCWWGG 3 cut(s) 108, 267, 501
FaeI CATG 7 cut(s) 271, 297, 357, 538, 573, 879, 917
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 328, 667
FatI CATG 7 cut(s) 267, 293, 353, 534, 569, 875, 913
FblI GTMKAC 1 cut(s) 735
Fnu4HI GCNGC 2 cut(s) 57, 1004
FokI GGATG 4 cut(s) 272, 686, 913, 1070
FriOI GRGCYC 3 cut(s) 138, 202, 585
Fsp4HI GCNGC 2 cut(s) 57, 1004
FspBI CTAG 3 cut(s) 458, 987, 1046
GluI GCNGC 2 cut(s) 57, 1004
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 3 cut(s) 500, 568, 806
HapII CCGG 1 cut(s) 993
Hin1II CATG 7 cut(s) 271, 297, 357, 538, 573, 879, 917
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 2 cut(s) 464, 981
HinfI GANTC 5 cut(s) 17, 119, 155, 540, 917
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 1 cut(s) 993
HphI GGTGA 5 cut(s) 517, 539, 549, 828, 1028
Hpy166II GTNNAC 3 cut(s) 530, 729, 736
Hpy188I TCNGA 3 cut(s) 178, 772, 961
Hpy188III TCNNGA 6 cut(s) 94, 116, 139, 458, 914, 1046
Hpy8I GTNNAC 3 cut(s) 530, 729, 736
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 466, 1043
HpyCH4III ACNGT 7 cut(s) 422, 589, 678, 692, 733, 828, 1120
HpyCH4IV ACGT 1 cut(s) 907
HpyCH4V TGCA 1 cut(s) 593
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 56, 65, 74, 206, 1009
HpyF3I CTNAG 4 cut(s) 201, 771, 897, 958
HpySE526I ACGT 1 cut(s) 907
Hsp92II CATG 7 cut(s) 271, 297, 357, 538, 573, 879, 917
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 6 cut(s) 112, 360, 475, 554, 887, 952
LmnI GCTCC 2 cut(s) 133, 250
Lsp1109I GCAGC 2 cut(s) 68, 1015
LweI GCATC 3 cut(s) 708, 842, 1121
MaeI CTAG 3 cut(s) 458, 987, 1046
MaeII ACGT 1 cut(s) 907
MaeIII GTNAC 3 cut(s) 692, 938, 1102
MalI GATC 6 cut(s) 114, 362, 477, 556, 889, 954
MboI GATC 6 cut(s) 112, 360, 475, 554, 887, 952
MboII GAAGA 2 cut(s) 674, 989
MflI RGATCY 2 cut(s) 112, 475
MhlI GDGCHC 3 cut(s) 138, 202, 585
MluCI AATT 7 cut(s) 192, 333, 367, 820, 1029, 1041, 1147
MlyI GAGTC 4 cut(s) 113, 149, 549, 926
MseI TTAA 8 cut(s) 29, 161, 332, 366, 495, 728, 860, 1125
MslI CAYNNNNRTG 1 cut(s) 948
MspI CCGG 1 cut(s) 993
MspR9I CCNGG 4 cut(s) 307, 322, 349, 993
MvaI CCWGG 3 cut(s) 307, 322, 349
MwoI GCNNNNNNNGC 6 cut(s) 41, 56, 65, 74, 206, 1009
NciI CCSGG 1 cut(s) 993
NcoI CCATGG 1 cut(s) 267
NdeII GATC 6 cut(s) 112, 360, 475, 554, 887, 952
NlaIII CATG 7 cut(s) 271, 297, 357, 538, 573, 879, 917
NlaIV GGNNCC 3 cut(s) 231, 273, 477
NmuCI GTSAC 2 cut(s) 692, 1102
NspI RCATGY 1 cut(s) 357
PaeI GCATGC 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 137
PagI TCATGA 1 cut(s) 913
PaqCI CACCTGC 1 cut(s) 856
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 2 cut(s) 58, 1005
PleI GAGTC 4 cut(s) 113, 149, 548, 925
PpsI GAGTC 4 cut(s) 113, 149, 548, 925
PshBI ATTAAT 1 cut(s) 332
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 305, 320, 347
PspEI GGTNACC 1 cut(s) 938
PspGI CCWGG 3 cut(s) 305, 320, 347
PspN4I GGNNCC 3 cut(s) 231, 273, 477
PspPI GGNCC 1 cut(s) 804
PstNI CAGNNNCTG 2 cut(s) 871, 1074
PsuI RGATCY 2 cut(s) 112, 475
RsaI GTAC 4 cut(s) 339, 663, 830, 1067
RsaNI GTAC 4 cut(s) 338, 662, 829, 1066
RseI CAYNNNNRTG 1 cut(s) 948
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 8 cut(s) 29, 161, 332, 366, 495, 728, 860, 1125
SatI GCNGC 2 cut(s) 57, 1004
Sau3AI GATC 6 cut(s) 112, 360, 475, 554, 887, 952
Sau96I GGNCC 1 cut(s) 804
SchI GAGTC 4 cut(s) 113, 149, 549, 926
ScrFI CCNGG 4 cut(s) 307, 322, 349, 993
SduI GDGCHC 3 cut(s) 138, 202, 585
SfaNI GCATC 3 cut(s) 708, 842, 1121
SfcI CTRYAG 1 cut(s) 12
Sfr274I CTCGAG 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 3 cut(s) 137, 149, 395
SmoI CTYRAG 3 cut(s) 137, 149, 395
SphI GCATGC 1 cut(s) 357
Sse9I AATT 7 cut(s) 192, 333, 367, 820, 1029, 1041, 1147
SsiI CCGC 3 cut(s) 66, 1010, 1141
SspMI CTAG 3 cut(s) 458, 987, 1046
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 4 cut(s) 305, 320, 347, 991
StyI CCWWGG 3 cut(s) 108, 267, 501
TaaI ACNGT 7 cut(s) 422, 589, 678, 692, 733, 828, 1120
TaiI ACGT 1 cut(s) 910
TaqI TCGA 5 cut(s) 138, 190, 488, 886, 947
TasI AATT 7 cut(s) 192, 333, 367, 820, 1029, 1041, 1147
TatI WGTACW 1 cut(s) 661
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 8 cut(s) 29, 161, 332, 366, 495, 728, 860, 1125
Tru9I TTAA 8 cut(s) 29, 161, 332, 366, 495, 728, 860, 1125
TscAI CASTG 6 cut(s) 79, 425, 592, 756, 795, 831
TseFI GTSAC 2 cut(s) 692, 1102
TseI GCWGC 2 cut(s) 56, 1003
Tsp45I GTSAC 2 cut(s) 692, 1102
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 6 cut(s) 79, 425, 592, 756, 795, 831
VspI ATTAAT 1 cut(s) 332
XapI RAATTY 2 cut(s) 192, 820
XbaI TCTAGA 2 cut(s) 457, 1045
XceI RCATGY 1 cut(s) 357
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 735
XspI CTAG 3 cut(s) 458, 987, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.