RchiOBHm_Chr4g0404161

Uncharacterised protein family UPF0066

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002784
Physical Location & Seq
Reverse (-)
23691530 .. 23699781
8252 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37578

Sequence Viewer

Length: 1152 bp
ATGGTTAAGAGCCACCATAGCCGTCGCACTCGCAGCTCTCTCCGCCTCCACTGCTCTTTCTCGTTGTATTTCTTGAAAAGAAAGTCCAAGGATGTGGACTCCAAGATCAGGGAGCTCGAGAAATCCCTCAAGGACTCCTTAAAGCACTGTGCTTCCGAAAGGCAAGGTCGAATTCGGGCTCAGCAGGCTTTGAGGAAATCGCTGACGGAACCAAAGGTTGATGATTTGGAGCTGACTTCTTACCCCATGGCACCTATTGGTGTCATCCAGTCATGCTTCTCCACCAGGAATGGGACACCCAGGCAACCATTAATTGTACCTCTTTCCAGGGCATGCTTGATCTTTAATTCAGCCCGTGTTCCTCCAGCTTCGCTTGAGGGTCTTGGAGACTACACGCACTGTTGGGTTATCTATGTTTTCCATCTAAATACAAATCTAGAGAAGCTTTGGAAGGATCCAGCAAAATCGAAGTTTAAGGCCAAGGTGAGAGTGCCAAGACTAAAAGGTGAACGCATGGGAGTCTTTGCCACCCGATCACCTCACAGGCCATGCCCCATTGGGCTCACTGTTGCAAAGGTGGAGGCTGTAAAAGGGAATATACTTCTACTCTCTGGTGTGGATTTGGTTGATGGGACGCCTGTACTTGATGTCAAACCGTATCTTCCTTACTGTGACAGCATCCAAGAAGCAGGAGTGCCAAAGTGGTTAACGGTAGACAGGTCATTTTCAGTGGCTTCTATTAGTTTCTCTGAGGGCTTCACTTCAACACTTGCTCAATGTTGGGCCATAACAGGTAAGAATTCACTGTACGCATCACCAGATGAGTTCCAAAACTTGTTAGAGCAGGTGCTGTCATGGGATATTCGATCAGTATCTCAGCGAACACGTCCTCATGAGTCCTTCATCACATCCCAAAATGGTAACCACTCGAATGATCTCTCAGATGACAACCAAGACGAAGAAGCTTCTAGCCCCGGAAACGAGCAGCCTCCGCAATCATCTGGCGACATAATTTATCACCTAATTCTAGAAGGATTGGATGTTTGGTACAGGCTCTGTGATGGGAATGTTGTTGTTGAGAAAGTGACAGAAGCATCAACCGTCATTAAAAGTGATCAAAAGCGGTGTAATTATTCTATATGGAGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0066

Protein Analysis

383

Amino Acids

43.08

Weight (kDa)

8.9

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmO_N PF01980 98 - 225 2.9e-38 tRNA-methyltransferase O N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 835
Acc36I ACCTGC 1 cut(s) 835
AccB1I GGYRCC 1 cut(s) 250
AccI GTMKAC 1 cut(s) 714
AciI CCGC 3 cut(s) 43, 992, 1123
AclWI GGATC 2 cut(s) 449, 462
AcsI RAATTY 2 cut(s) 171, 799
AcyI GRCGYC 1 cut(s) 635
AfaI GTAC 4 cut(s) 318, 642, 809, 1049
AfiI CCNNNNNNNGG 2 cut(s) 108, 291
AflIII ACRYGT 1 cut(s) 884
AgsI TTSAA 2 cut(s) 76, 765
AjiI CACGTC 1 cut(s) 887
AjnI CCWGG 3 cut(s) 284, 299, 326
AluBI AGCT 6 cut(s) 36, 115, 232, 368, 445, 965
AluI AGCT 6 cut(s) 36, 115, 232, 368, 445, 965
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 381, 1140
AlwI GGATC 2 cut(s) 449, 462
AlwNI CAGNNNCTG 2 cut(s) 850, 1056
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 3 cut(s) 477, 545, 783
ApeKI GCWGC 2 cut(s) 33, 985
ApoI RAATTY 2 cut(s) 171, 799
AseI ATTAAT 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 783
AsuC2I CCSGG 1 cut(s) 975
AsuHPI GGTGA 5 cut(s) 496, 518, 528, 807, 1010
AvaI CYCGRG 1 cut(s) 116
BamHI GGATCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 250
BanII GRGCYC 3 cut(s) 117, 181, 564
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 45, 997
BccI CCATC 3 cut(s) 429, 623, 1055
BceAI ACGGC 1 cut(s) 6
BciT130I CCWGG 3 cut(s) 286, 301, 328
BclI TGATCA 1 cut(s) 1114
BcnI CCSGG 1 cut(s) 975
BcoDI GTCTC 2 cut(s) 381, 1140
BfaI CTAG 3 cut(s) 437, 969, 1028
BfuAI ACCTGC 1 cut(s) 835
BisI GCNGC 2 cut(s) 34, 986
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 2 cut(s) 35, 987
Bme1390I CCNGG 4 cut(s) 286, 301, 328, 975
BmeT110I CYCGRG 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 887
BmgT120I GGNCC 1 cut(s) 783
BmiI GGNNCC 3 cut(s) 210, 252, 456
BmrFI CCNGG 4 cut(s) 286, 301, 328, 975
BmsI GCATC 3 cut(s) 687, 821, 1103
BpmI CTGGAG 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 180
BpuEI CTTGAG 2 cut(s) 113, 395
BpuMI CCSGG 1 cut(s) 975
BsaBI GATNNNNATC 1 cut(s) 871
BsaHI GRCGYC 1 cut(s) 635
BsaJI CCNNGG 6 cut(s) 87, 246, 299, 327, 480, 973
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 291
Bse1I ACTGG 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 871
BseBI CCWGG 3 cut(s) 286, 301, 328
BseDI CCNNGG 6 cut(s) 87, 246, 299, 327, 480, 973
BseGI GGATG 5 cut(s) 97, 264, 678, 908, 1045
BseJI GATNNNNATC 1 cut(s) 871
BseLI CCNNNNNNNGG 2 cut(s) 108, 291
BseMII CTCAG 4 cut(s) 194, 741, 890, 954
BseNI ACTGG 1 cut(s) 268
BseXI GCAGC 2 cut(s) 45, 997
BshFI GGCC 3 cut(s) 479, 547, 785
BshNI GGYRCC 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 975
BslFI GGGAC 2 cut(s) 307, 646
BslI CCNNNNNNNGG 2 cut(s) 108, 291
BsmAI GTCTC 2 cut(s) 381, 1140
BsmFI GGGAC 2 cut(s) 307, 646
BsnI GGCC 3 cut(s) 479, 547, 785
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 3 cut(s) 117, 181, 564
Bsp143I GATC 7 cut(s) 105, 339, 454, 533, 866, 934, 1114
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 3 cut(s) 43, 992, 1123
BspANI GGCC 3 cut(s) 479, 547, 785
BspCNI CTCAG 4 cut(s) 193, 742, 889, 953
BspHI TCATGA 1 cut(s) 892
BspLI GGNNCC 3 cut(s) 210, 252, 456
BspMI ACCTGC 1 cut(s) 835
BspPI GGATC 2 cut(s) 449, 462
BspT107I GGYRCC 1 cut(s) 250
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 6 cut(s) 87, 246, 299, 327, 480, 973
BssMI GATC 7 cut(s) 105, 339, 454, 533, 866, 934, 1114
BssNI GRCGYC 1 cut(s) 635
BssT1I CCWWGG 3 cut(s) 87, 246, 480
Bst2UI CCWGG 3 cut(s) 286, 301, 328
Bst4CI ACNGT 8 cut(s) 149, 401, 568, 657, 671, 712, 807, 1102
BstACI GRCGYC 1 cut(s) 635
BstC8I GCNNGC 2 cut(s) 186, 334
BstDEI CTNAG 4 cut(s) 180, 750, 876, 940
BstDSI CCRYGG 1 cut(s) 246
BstEII GGTNACC 1 cut(s) 920
BstF5I GGATG 5 cut(s) 97, 264, 678, 908, 1045
BstKTI GATC 7 cut(s) 108, 342, 457, 536, 869, 937, 1117
BstMAI GTCTC 2 cut(s) 381, 1140
BstMBI GATC 7 cut(s) 105, 339, 454, 533, 866, 934, 1114
BstMWI GCNNNNNNNGC 6 cut(s) 18, 33, 42, 51, 185, 991
BstNI CCWGG 3 cut(s) 286, 301, 328
BstNSI RCATGY 1 cut(s) 336
BstPI GGTNACC 1 cut(s) 920
BstSCI CCNGG 4 cut(s) 284, 299, 326, 973
BstV1I GCAGC 2 cut(s) 45, 997
BstX2I RGATCY 1 cut(s) 454
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 454
BsuRI GGCC 3 cut(s) 479, 547, 785
BtgI CCRYGG 1 cut(s) 246
BtrI CACGTC 1 cut(s) 887
BtsCI GGATG 5 cut(s) 97, 264, 678, 908, 1045
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 6 cut(s) 49, 145, 397, 564, 735, 803
BveI ACCTGC 1 cut(s) 835
Cac8I GCNNGC 2 cut(s) 186, 334
CaiI CAGNNNCTG 2 cut(s) 850, 1056
CciI TCATGA 1 cut(s) 892
Cfr13I GGNCC 1 cut(s) 783
CseI GACGC 1 cut(s) 643
Csp6I GTAC 4 cut(s) 317, 641, 808, 1048
CviAII CATG 7 cut(s) 247, 273, 333, 514, 549, 855, 893
CviQI GTAC 4 cut(s) 317, 641, 808, 1048
DdeI CTNAG 4 cut(s) 180, 750, 876, 940
DpnI GATC 7 cut(s) 107, 341, 456, 535, 868, 936, 1116
DpnII GATC 7 cut(s) 105, 339, 454, 533, 866, 934, 1114
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 3 cut(s) 87, 246, 480
Eco24I GRGCYC 3 cut(s) 117, 181, 564
Eco53kI GAGCTC 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 116
Eco91I GGTNACC 1 cut(s) 920
EcoICRI GAGCTC 1 cut(s) 115
EcoO65I GGTNACC 1 cut(s) 920
EcoRI GAATTC 2 cut(s) 171, 799
EcoRII CCWGG 3 cut(s) 284, 299, 326
EcoT14I CCWWGG 3 cut(s) 87, 246, 480
EcoT38I GRGCYC 3 cut(s) 117, 181, 564
ErhI CCWWGG 3 cut(s) 87, 246, 480
FaeI CATG 7 cut(s) 250, 276, 336, 517, 552, 858, 896
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 2 cut(s) 307, 646
FatI CATG 7 cut(s) 246, 272, 332, 513, 548, 854, 892
FbaI TGATCA 1 cut(s) 1114
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 2 cut(s) 34, 986
FokI GGATG 5 cut(s) 104, 251, 665, 895, 1052
FriOI GRGCYC 3 cut(s) 117, 181, 564
Fsp4HI GCNGC 2 cut(s) 34, 986
FspBI CTAG 3 cut(s) 437, 969, 1028
GluI GCNGC 2 cut(s) 34, 986
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 3 cut(s) 479, 547, 785
HapII CCGG 1 cut(s) 975
HgaI GACGC 1 cut(s) 643
Hin1I GRCGYC 1 cut(s) 635
Hin1II CATG 7 cut(s) 250, 276, 336, 517, 552, 858, 896
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HindIII AAGCTT 2 cut(s) 443, 963
HinfI GANTC 4 cut(s) 98, 134, 519, 896
HpaI GTTAAC 1 cut(s) 708
HpaII CCGG 1 cut(s) 975
HphI GGTGA 5 cut(s) 496, 518, 528, 807, 1010
Hpy166II GTNNAC 4 cut(s) 97, 509, 708, 715
Hpy188I TCNGA 3 cut(s) 157, 751, 943
Hpy188III TCNNGA 5 cut(s) 73, 118, 437, 893, 1028
Hpy8I GTNNAC 4 cut(s) 97, 509, 708, 715
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 3 cut(s) 445, 910, 1025
HpyCH4III ACNGT 8 cut(s) 149, 401, 568, 657, 671, 712, 807, 1102
HpyCH4IV ACGT 1 cut(s) 886
HpyCH4V TGCA 1 cut(s) 572
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 33, 42, 51, 185, 991
HpyF3I CTNAG 4 cut(s) 180, 750, 876, 940
HpySE526I ACGT 1 cut(s) 886
Hsp92I GRCGYC 1 cut(s) 635
Hsp92II CATG 7 cut(s) 250, 276, 336, 517, 552, 858, 896
Ksp22I TGATCA 1 cut(s) 1114
KspAI GTTAAC 1 cut(s) 708
Kzo9I GATC 7 cut(s) 105, 339, 454, 533, 866, 934, 1114
LmnI GCTCC 2 cut(s) 112, 229
Lsp1109I GCAGC 2 cut(s) 45, 997
LweI GCATC 3 cut(s) 687, 821, 1103
MaeI CTAG 3 cut(s) 437, 969, 1028
MaeII ACGT 1 cut(s) 886
MaeIII GTNAC 3 cut(s) 671, 920, 1084
MalI GATC 7 cut(s) 107, 341, 456, 535, 868, 936, 1116
MboI GATC 7 cut(s) 105, 339, 454, 533, 866, 934, 1114
MboII GAAGA 2 cut(s) 653, 971
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 3 cut(s) 117, 181, 564
MluCI AATT 7 cut(s) 171, 312, 346, 799, 1011, 1023, 1129
MlyI GAGTC 4 cut(s) 92, 128, 528, 905
MseI TTAA 7 cut(s) 6, 140, 311, 345, 474, 707, 1107
MslI CAYNNNNRTG 1 cut(s) 930
MspI CCGG 1 cut(s) 975
MspR9I CCNGG 4 cut(s) 286, 301, 328, 975
MvaI CCWGG 3 cut(s) 286, 301, 328
MwoI GCNNNNNNNGC 6 cut(s) 18, 33, 42, 51, 185, 991
NciI CCSGG 1 cut(s) 975
NcoI CCATGG 1 cut(s) 246
NdeII GATC 7 cut(s) 105, 339, 454, 533, 866, 934, 1114
NlaIII CATG 7 cut(s) 250, 276, 336, 517, 552, 858, 896
NlaIV GGNNCC 3 cut(s) 210, 252, 456
NmuCI GTSAC 2 cut(s) 671, 1084
NspI RCATGY 1 cut(s) 336
PaeI GCATGC 1 cut(s) 336
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 1 cut(s) 892
PaqCI CACCTGC 1 cut(s) 835
PkrI GCNGC 2 cut(s) 35, 987
PleI GAGTC 4 cut(s) 92, 128, 527, 904
PpsI GAGTC 4 cut(s) 92, 128, 527, 904
PshBI ATTAAT 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 3 cut(s) 284, 299, 326
PspEI GGTNACC 1 cut(s) 920
PspGI CCWGG 3 cut(s) 284, 299, 326
PspN4I GGNNCC 3 cut(s) 210, 252, 456
PspPI GGNCC 1 cut(s) 783
PstNI CAGNNNCTG 2 cut(s) 850, 1056
PsuI RGATCY 1 cut(s) 454
RsaI GTAC 4 cut(s) 318, 642, 809, 1049
RsaNI GTAC 4 cut(s) 317, 641, 808, 1048
RseI CAYNNNNRTG 1 cut(s) 930
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 7 cut(s) 6, 140, 311, 345, 474, 707, 1107
SatI GCNGC 2 cut(s) 34, 986
Sau3AI GATC 7 cut(s) 105, 339, 454, 533, 866, 934, 1114
Sau96I GGNCC 1 cut(s) 783
SchI GAGTC 4 cut(s) 92, 128, 528, 905
ScrFI CCNGG 4 cut(s) 286, 301, 328, 975
SduI GDGCHC 3 cut(s) 117, 181, 564
SfaNI GCATC 3 cut(s) 687, 821, 1103
Sfr274I CTCGAG 1 cut(s) 116
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 930
SmlI CTYRAG 3 cut(s) 116, 128, 374
SmoI CTYRAG 3 cut(s) 116, 128, 374
SphI GCATGC 1 cut(s) 336
Sse9I AATT 7 cut(s) 171, 312, 346, 799, 1011, 1023, 1129
SsiI CCGC 3 cut(s) 43, 992, 1123
SspMI CTAG 3 cut(s) 437, 969, 1028
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 4 cut(s) 284, 299, 326, 973
StyI CCWWGG 3 cut(s) 87, 246, 480
TaaI ACNGT 8 cut(s) 149, 401, 568, 657, 671, 712, 807, 1102
TaiI ACGT 1 cut(s) 889
TaqI TCGA 5 cut(s) 117, 169, 467, 865, 929
TasI AATT 7 cut(s) 171, 312, 346, 799, 1011, 1023, 1129
TatI WGTACW 1 cut(s) 640
Tru1I TTAA 7 cut(s) 6, 140, 311, 345, 474, 707, 1107
Tru9I TTAA 7 cut(s) 6, 140, 311, 345, 474, 707, 1107
TscAI CASTG 6 cut(s) 56, 152, 404, 571, 735, 810
TseFI GTSAC 2 cut(s) 671, 1084
TseI GCWGC 2 cut(s) 33, 985
Tsp45I GTSAC 2 cut(s) 671, 1084
TspDTI ATGAA 1 cut(s) 892
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 6 cut(s) 56, 152, 404, 571, 735, 810
VspI ATTAAT 1 cut(s) 311
XapI RAATTY 2 cut(s) 171, 799
XbaI TCTAGA 2 cut(s) 436, 1027
XceI RCATGY 1 cut(s) 336
XhoI CTCGAG 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 714
XspI CTAG 3 cut(s) 437, 969, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.