FvH4_4g08360

Uncharacterised protein family UPF0066

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002784
Physical Location & Seq
Forward (+)
8297695 .. 8304575
6881 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08360.t12

Sequence Viewer

Length: 762 bp
ATGGGTTTCGTTACAGATTCCAAATTGTTAAGAGCCACCATAGCCGTCGCCCTCGCAGCTCTCTCCGCCTCCGCTGCACTCTCTCTGCATTTCATGAGAAGAAAATCGAAGGATCTGGACTCCAAGATTAGGGAGCTCGAGAAATCCCTCAAGGCCTCCTTGAAGCATTGCGCTTCCGAAAGGCAAGGTCGAATTCGGGCTCAGCAGGCTTTGAGGAAATCGCTGACGGAACCAAAGGTTGATGATTTGGAGCTGACTTGTTACCCCATGGCACCTATTGGTGTCATCCAGTCCTGCTTCTCTACCAGGAATGGGACGCCCAGGCAACCATTAATTGTACCTCTTTCCAGGGCATGCTTGATCTTTAATTCAGCCCGTGTTCCTCCAGCTTCGCTTGAGGGTCTTGGAGACTACACGCATTGTTGGGTTATCTATGTTTTCCATCTAAATACAAATCTAGAGAGGCTTTGGAAGAATCCAGCTAAATCAAAGTTTAAGGCCAAGGTGAGAGTGCCAAGACTAAAAGGTGAACGCATGGGAGTCTTTGCCACCCGATCACCCCACAGGCCATGCCCCATTGGGCTCACTGTTGCAAAAGTAGAGGCTGTAAAAGGAAATATACTTCTACTCTCTGGTGTGGATTTGGTTGATGGGACACCTGTACTTGATGTCAAACCATATCTTCCTTACTGCGACAGCATCCAGGAAGCAGGAGTGCCAAAATGGTTAATGGTATTATTTCCATGGTCTTTCAAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0066

Protein Analysis

254

Amino Acids

28.15

Weight (kDa)

9.94

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmO_N PF01980 74 - 171 5.1e-22 tRNA-methyltransferase O N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AciI CCGC 2 cut(s) 66, 72
AclWI GGATC 1 cut(s) 120
AcsI RAATTY 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 2 cut(s) 339, 663
AfiI CCNNNNNNNGG 2 cut(s) 129, 312
AgsI TTSAA 2 cut(s) 163, 754
AjnI CCWGG 4 cut(s) 305, 320, 347, 702
AluBI AGCT 5 cut(s) 59, 136, 253, 389, 482
AluI AGCT 5 cut(s) 59, 136, 253, 389, 482
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 1 cut(s) 120
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 3 cut(s) 153, 498, 566
ApeKI GCWGC 2 cut(s) 56, 74
ApoI RAATTY 1 cut(s) 192
AseI ATTAAT 1 cut(s) 332
AspLEI GCGC 1 cut(s) 173
AsuHPI GGTGA 3 cut(s) 517, 539, 549
AvaI CYCGRG 1 cut(s) 137
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 3 cut(s) 138, 202, 585
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 61, 68
BccI CCATC 2 cut(s) 450, 644
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 4 cut(s) 307, 322, 349, 704
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 1 cut(s) 458
BisI GCNGC 2 cut(s) 57, 75
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 2 cut(s) 58, 76
Bme1390I CCNGG 4 cut(s) 307, 322, 349, 704
BmeT110I CYCGRG 1 cut(s) 137
BmiI GGNNCC 2 cut(s) 231, 273
BmrFI CCNGG 4 cut(s) 307, 322, 349, 704
BmsI GCATC 1 cut(s) 708
BpmI CTGGAG 1 cut(s) 369
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 134, 416
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 5 cut(s) 267, 320, 348, 501, 743
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 312
Bse1I ACTGG 1 cut(s) 289
Bse3DI GCAATG 1 cut(s) 166
BseBI CCWGG 4 cut(s) 307, 322, 349, 704
BseDI CCNNGG 5 cut(s) 267, 320, 348, 501, 743
BseGI GGATG 2 cut(s) 285, 699
BseLI CCNNNNNNNGG 2 cut(s) 129, 312
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 289
BseXI GCAGC 2 cut(s) 61, 68
BsgI GTGCAG 1 cut(s) 60
BshFI GGCC 3 cut(s) 155, 500, 568
BshNI GGYRCC 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 137
BslFI GGGAC 2 cut(s) 328, 667
BslI CCNNNNNNNGG 2 cut(s) 129, 312
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 2 cut(s) 328, 667
BsnI GGCC 3 cut(s) 155, 500, 568
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 3 cut(s) 138, 202, 585
Bsp143I GATC 3 cut(s) 112, 360, 554
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 2 cut(s) 267, 743
BspACI CCGC 2 cut(s) 66, 72
BspANI GGCC 3 cut(s) 155, 500, 568
BspCNI CTCAG 1 cut(s) 214
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 231, 273
BspPI GGATC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 271
BsrDI GCAATG 1 cut(s) 166
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 5 cut(s) 267, 320, 348, 501, 743
BssMI GATC 3 cut(s) 112, 360, 554
BssNI GRCGYC 1 cut(s) 317
BssT1I CCWWGG 3 cut(s) 267, 501, 743
Bst2UI CCWGG 4 cut(s) 307, 322, 349, 704
Bst4CI ACNGT 1 cut(s) 589
BstACI GRCGYC 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 207, 355
BstDEI CTNAG 1 cut(s) 201
BstDSI CCRYGG 2 cut(s) 267, 743
BstF5I GGATG 2 cut(s) 285, 699
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 3 cut(s) 115, 363, 557
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 3 cut(s) 112, 360, 554
BstMWI GCNNNNNNNGC 5 cut(s) 41, 56, 65, 74, 206
BstNI CCWGG 4 cut(s) 307, 322, 349, 704
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 4 cut(s) 305, 320, 347, 702
BstV1I GCAGC 2 cut(s) 61, 68
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
BsuRI GGCC 3 cut(s) 155, 500, 568
BtgI CCRYGG 2 cut(s) 267, 743
BtsCI GGATG 2 cut(s) 285, 699
BtsIMutI CAGTG 1 cut(s) 585
Cac8I GCNNGC 2 cut(s) 207, 355
CciI TCATGA 1 cut(s) 93
CfoI GCGC 1 cut(s) 173
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 338, 662
CviAII CATG 6 cut(s) 94, 268, 354, 535, 570, 744
CviQI GTAC 2 cut(s) 338, 662
DdeI CTNAG 1 cut(s) 201
DpnI GATC 3 cut(s) 114, 362, 556
DpnII GATC 3 cut(s) 112, 360, 554
EciI GGCGGA 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 267, 501, 743
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 3 cut(s) 138, 202, 585
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 192
EcoRII CCWGG 4 cut(s) 305, 320, 347, 702
EcoT14I CCWWGG 3 cut(s) 267, 501, 743
EcoT38I GRGCYC 3 cut(s) 138, 202, 585
ErhI CCWWGG 3 cut(s) 267, 501, 743
FaeI CATG 6 cut(s) 97, 271, 357, 538, 573, 747
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 328, 667
FatI CATG 6 cut(s) 93, 267, 353, 534, 569, 743
Fnu4HI GCNGC 2 cut(s) 57, 75
FokI GGATG 2 cut(s) 272, 686
FriOI GRGCYC 3 cut(s) 138, 202, 585
Fsp4HI GCNGC 2 cut(s) 57, 75
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 172
GluI GCNGC 2 cut(s) 57, 75
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 3 cut(s) 155, 500, 568
HgaI GACGC 1 cut(s) 325
HhaI GCGC 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 6 cut(s) 97, 271, 357, 538, 573, 747
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HinfI GANTC 4 cut(s) 17, 119, 475, 540
HphI GGTGA 3 cut(s) 517, 539, 549
Hpy166II GTNNAC 1 cut(s) 530
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 5 cut(s) 94, 116, 139, 458, 754
Hpy8I GTNNAC 1 cut(s) 530
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 1 cut(s) 589
HpyCH4V TGCA 3 cut(s) 77, 88, 593
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 56, 65, 74, 206
HpyF3I CTNAG 1 cut(s) 201
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 6 cut(s) 97, 271, 357, 538, 573, 747
HspAI GCGC 1 cut(s) 171
Kzo9I GATC 3 cut(s) 112, 360, 554
LmnI GCTCC 2 cut(s) 133, 250
Lsp1109I GCAGC 2 cut(s) 61, 68
LweI GCATC 1 cut(s) 708
MaeI CTAG 1 cut(s) 458
MaeIII GTNAC 2 cut(s) 10, 260
MalI GATC 3 cut(s) 114, 362, 556
MboI GATC 3 cut(s) 112, 360, 554
MboII GAAGA 3 cut(s) 111, 484, 674
MflI RGATCY 1 cut(s) 112
MhlI GDGCHC 3 cut(s) 138, 202, 585
MluCI AATT 4 cut(s) 23, 192, 333, 367
MlyI GAGTC 2 cut(s) 113, 549
MseI TTAA 5 cut(s) 29, 332, 366, 495, 728
MspA1I CMGCKG 1 cut(s) 74
MspR9I CCNGG 4 cut(s) 307, 322, 349, 704
MvaI CCWGG 4 cut(s) 307, 322, 349, 704
MwoI GCNNNNNNNGC 5 cut(s) 41, 56, 65, 74, 206
NcoI CCATGG 2 cut(s) 267, 743
NdeII GATC 3 cut(s) 112, 360, 554
NlaIII CATG 6 cut(s) 97, 271, 357, 538, 573, 747
NlaIV GGNNCC 2 cut(s) 231, 273
NspI RCATGY 1 cut(s) 357
PaeI GCATGC 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 137
PagI TCATGA 1 cut(s) 93
PceI AGGCCT 1 cut(s) 155
PfeI GAWTC 2 cut(s) 17, 475
PfoI TCCNGGA 1 cut(s) 702
PkrI GCNGC 2 cut(s) 58, 76
PleI GAGTC 2 cut(s) 113, 548
PpsI GAGTC 2 cut(s) 113, 548
PshBI ATTAAT 1 cut(s) 332
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 4 cut(s) 305, 320, 347, 702
PspGI CCWGG 4 cut(s) 305, 320, 347, 702
PspN4I GGNNCC 2 cut(s) 231, 273
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 2 cut(s) 339, 663
RsaNI GTAC 2 cut(s) 338, 662
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 5 cut(s) 29, 332, 366, 495, 728
SatI GCNGC 2 cut(s) 57, 75
Sau3AI GATC 3 cut(s) 112, 360, 554
SchI GAGTC 2 cut(s) 113, 549
ScrFI CCNGG 4 cut(s) 307, 322, 349, 704
SduI GDGCHC 3 cut(s) 138, 202, 585
SfaNI GCATC 1 cut(s) 708
Sfr274I CTCGAG 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 137
SmlI CTYRAG 3 cut(s) 137, 149, 395
SmoI CTYRAG 3 cut(s) 137, 149, 395
SphI GCATGC 1 cut(s) 357
Sse9I AATT 4 cut(s) 23, 192, 333, 367
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 2 cut(s) 66, 72
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 4 cut(s) 305, 320, 347, 702
StyI CCWWGG 3 cut(s) 267, 501, 743
TaaI ACNGT 1 cut(s) 589
TaqI TCGA 3 cut(s) 107, 138, 190
TasI AATT 4 cut(s) 23, 192, 333, 367
TatI WGTACW 1 cut(s) 661
TfiI GAWTC 2 cut(s) 17, 475
Tru1I TTAA 5 cut(s) 29, 332, 366, 495, 728
Tru9I TTAA 5 cut(s) 29, 332, 366, 495, 728
TscAI CASTG 1 cut(s) 592
TseI GCWGC 2 cut(s) 56, 74
TspDTI ATGAA 1 cut(s) 82
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 1 cut(s) 592
VspI ATTAAT 1 cut(s) 332
XapI RAATTY 1 cut(s) 192
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 1 cut(s) 357
XhoI CTCGAG 1 cut(s) 137
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.