Rw4G009990

Uncharacterised protein family UPF0066

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002784
Physical Location & Seq
Reverse (-)
22790791 .. 22799046
8256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009990.1

Sequence Viewer

Length: 1173 bp
ATGGGTTTCGCTACAGAATCCAAATGGTTAAGAGCCACCATAGCCGTCGCACTCGCAGCTCTCTCCGCCTCCACTGCTCTTTCTCTGTATTTCTTGAAAAGAAAGTCCAAGGATCTGGACTCCAAGATCAGGGAGCTCGAGAAATCCCTCAAGGACTCCTTAAAGCACTGTGCTTCCGAAAGGCAAGGTCGAATTCGGGCTCAGCAGGCTTTGAGGAAATCGCTGACGGAACCAAAGGTTGATGATTTGGAGCTGACTTCTTACCCCATGGCACCTATTGGTGTCATCCAGTCATGCTTCTCCACCAGGAATGGGACACCCAGGCAACCATTAATTGTACCTCTTTCCAGGGCATGCTTGATCTTTAATTCAGCCCGTGTTCCTCCAGCTTCACTTGAGGGTCTTGGAGACTACACGCACTGTTGGGTTATCTATGTTTTCCATCTAAATACAAATCTAGAGAAGCTTTGGAAGGATCCAGCAAAATCGAAGTTTAAGGCCAAGGTGAGAGTGCCAAGACTAAAAGGTGAACGCATGGGAGTCTTTGCCACCCGATCACCACACAGGCCATGCCCCATTGGGCTCACAGTTGCAAAGGTGGAGGCTGTAAAAGGGAATATACTTCTACTCTCTGGTGTGGATTTGGTTGATGGGACGCCTGTACTTGATGTCAAACCGTATCTTCCTTACTGTGACAGCATCCAAGAAGCAGGAGTGCCAAAGTGGTTAACGGTAGACAGGTCATTTTCAGTGGCTTCTATTAGTTTCTCTGAGGGCTTCACTTCAACACTTGCTCAATGTTGGGCCATAACAGGTAAGAATTCACTGTACGCATCACCAGATGAGTTCCAAAACTTGTTAGAGCAGGTGCTGTCGTGGGATATTCGATCAGTATCTCAGCGAACACGTCCTCATGAGTCCTTCATCACATCCCAAAGTGGTAACCACTCGAATGATCTCTCAGATGACTACCAAGACGAAGAAGCTTCTAGCCCCGGAAACGAGCAGCCTCCGCAATCATCTGGCGACATAATTTATCACCTAATTCTAGAAGGATTGGATGTTTGGTACAGGCTCTGTGATGGGAATGTTGTTGTTGAGAAAGTGACAGAACCATCAACCGTCATTAAAAGTAATCAAAAGCGGTGTAATTACTCTATATGGAGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0066

Protein Analysis

390

Amino Acids

43.46

Weight (kDa)

8.61

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TrmO_N PF01980 105 - 232 3e-38 tRNA-methyltransferase O N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 856
Acc36I ACCTGC 1 cut(s) 856
AccB1I GGYRCC 1 cut(s) 271
AccI GTMKAC 1 cut(s) 735
AciI CCGC 3 cut(s) 66, 1013, 1144
AclWI GGATC 3 cut(s) 120, 470, 483
AcsI RAATTY 2 cut(s) 192, 820
AcyI GRCGYC 1 cut(s) 656
AfaI GTAC 4 cut(s) 339, 663, 830, 1070
AfiI CCNNNNNNNGG 2 cut(s) 129, 312
AflIII ACRYGT 1 cut(s) 905
AgsI TTSAA 2 cut(s) 97, 786
AjiI CACGTC 1 cut(s) 908
AjnI CCWGG 3 cut(s) 305, 320, 347
AluBI AGCT 6 cut(s) 59, 136, 253, 389, 466, 986
AluI AGCT 6 cut(s) 59, 136, 253, 389, 466, 986
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 402, 1161
AlwI GGATC 3 cut(s) 120, 470, 483
AlwNI CAGNNNCTG 2 cut(s) 871, 1077
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 3 cut(s) 498, 566, 804
ApeKI GCWGC 2 cut(s) 56, 1006
ApoI RAATTY 2 cut(s) 192, 820
AseI ATTAAT 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 804
AsuC2I CCSGG 1 cut(s) 996
AsuHPI GGTGA 5 cut(s) 517, 539, 549, 828, 1031
AvaI CYCGRG 1 cut(s) 137
BamHI GGATCC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 3 cut(s) 138, 202, 585
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 68, 1018
BccI CCATC 4 cut(s) 450, 644, 1076, 1123
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 307, 322, 349
BcnI CCSGG 1 cut(s) 996
BcoDI GTCTC 2 cut(s) 402, 1161
BfaI CTAG 3 cut(s) 458, 990, 1049
BfmI CTRYAG 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 856
BisI GCNGC 2 cut(s) 57, 1007
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 2 cut(s) 58, 1008
Bme1390I CCNGG 4 cut(s) 307, 322, 349, 996
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 908
BmgT120I GGNCC 1 cut(s) 804
BmiI GGNNCC 3 cut(s) 231, 273, 477
BmrFI CCNGG 4 cut(s) 307, 322, 349, 996
BmsI GCATC 2 cut(s) 708, 842
BpmI CTGGAG 1 cut(s) 369
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 134, 416
BpuMI CCSGG 1 cut(s) 996
BsaBI GATNNNNATC 1 cut(s) 892
BsaHI GRCGYC 1 cut(s) 656
BsaJI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 994
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 312
Bse1I ACTGG 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 892
BseBI CCWGG 3 cut(s) 307, 322, 349
BseDI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 994
BseGI GGATG 4 cut(s) 285, 699, 929, 1066
BseJI GATNNNNATC 1 cut(s) 892
BseLI CCNNNNNNNGG 2 cut(s) 129, 312
BseMII CTCAG 4 cut(s) 215, 762, 911, 975
BseNI ACTGG 1 cut(s) 289
BseXI GCAGC 2 cut(s) 68, 1018
BshFI GGCC 3 cut(s) 500, 568, 806
BshNI GGYRCC 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 996
BslFI GGGAC 2 cut(s) 328, 667
BslI CCNNNNNNNGG 2 cut(s) 129, 312
BsmAI GTCTC 2 cut(s) 402, 1161
BsmFI GGGAC 2 cut(s) 328, 667
BsnI GGCC 3 cut(s) 500, 568, 806
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 3 cut(s) 138, 202, 585
Bsp143I GATC 7 cut(s) 112, 126, 360, 475, 554, 887, 955
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 3 cut(s) 66, 1013, 1144
BspANI GGCC 3 cut(s) 500, 568, 806
BspCNI CTCAG 4 cut(s) 214, 763, 910, 974
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 3 cut(s) 231, 273, 477
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 3 cut(s) 120, 470, 483
BspT107I GGYRCC 1 cut(s) 271
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 6 cut(s) 108, 267, 320, 348, 501, 994
BssMI GATC 7 cut(s) 112, 126, 360, 475, 554, 887, 955
BssNI GRCGYC 1 cut(s) 656
BssT1I CCWWGG 3 cut(s) 108, 267, 501
Bst2UI CCWGG 3 cut(s) 307, 322, 349
Bst4CI ACNGT 8 cut(s) 170, 422, 589, 678, 692, 733, 828, 1123
BstACI GRCGYC 1 cut(s) 656
BstC8I GCNNGC 2 cut(s) 207, 355
BstDEI CTNAG 4 cut(s) 201, 771, 897, 961
BstDSI CCRYGG 1 cut(s) 267
BstEII GGTNACC 1 cut(s) 941
BstF5I GGATG 4 cut(s) 285, 699, 929, 1066
BstKTI GATC 7 cut(s) 115, 129, 363, 478, 557, 890, 958
BstMAI GTCTC 2 cut(s) 402, 1161
BstMBI GATC 7 cut(s) 112, 126, 360, 475, 554, 887, 955
BstMWI GCNNNNNNNGC 6 cut(s) 41, 56, 65, 74, 206, 1012
BstNI CCWGG 3 cut(s) 307, 322, 349
BstNSI RCATGY 1 cut(s) 357
BstPI GGTNACC 1 cut(s) 941
BstSCI CCNGG 4 cut(s) 305, 320, 347, 994
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 68, 1018
BstX2I RGATCY 2 cut(s) 112, 475
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 2 cut(s) 112, 475
BsuRI GGCC 3 cut(s) 500, 568, 806
BtgI CCRYGG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 908
BtsCI GGATG 4 cut(s) 285, 699, 929, 1066
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 5 cut(s) 72, 166, 418, 756, 824
BveI ACCTGC 1 cut(s) 856
Cac8I GCNNGC 2 cut(s) 207, 355
CaiI CAGNNNCTG 2 cut(s) 871, 1077
CciI TCATGA 1 cut(s) 913
Cfr13I GGNCC 1 cut(s) 804
CseI GACGC 1 cut(s) 664
Csp6I GTAC 4 cut(s) 338, 662, 829, 1069
CviAII CATG 6 cut(s) 268, 294, 354, 535, 570, 914
CviQI GTAC 4 cut(s) 338, 662, 829, 1069
DdeI CTNAG 4 cut(s) 201, 771, 897, 961
DpnI GATC 7 cut(s) 114, 128, 362, 477, 556, 889, 957
DpnII GATC 7 cut(s) 112, 126, 360, 475, 554, 887, 955
EciI GGCGGA 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 108, 267, 501
Eco24I GRGCYC 3 cut(s) 138, 202, 585
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 941
EcoICRI GAGCTC 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 941
EcoRI GAATTC 2 cut(s) 192, 820
EcoRII CCWGG 3 cut(s) 305, 320, 347
EcoT14I CCWWGG 3 cut(s) 108, 267, 501
EcoT38I GRGCYC 3 cut(s) 138, 202, 585
ErhI CCWWGG 3 cut(s) 108, 267, 501
FaeI CATG 6 cut(s) 271, 297, 357, 538, 573, 917
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 328, 667
FatI CATG 6 cut(s) 267, 293, 353, 534, 569, 913
FblI GTMKAC 1 cut(s) 735
Fnu4HI GCNGC 2 cut(s) 57, 1007
FokI GGATG 4 cut(s) 272, 686, 916, 1073
FriOI GRGCYC 3 cut(s) 138, 202, 585
Fsp4HI GCNGC 2 cut(s) 57, 1007
FspBI CTAG 3 cut(s) 458, 990, 1049
GluI GCNGC 2 cut(s) 57, 1007
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 3 cut(s) 500, 568, 806
HapII CCGG 1 cut(s) 996
HgaI GACGC 1 cut(s) 664
Hin1I GRCGYC 1 cut(s) 656
Hin1II CATG 6 cut(s) 271, 297, 357, 538, 573, 917
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 2 cut(s) 464, 984
HinfI GANTC 5 cut(s) 17, 119, 155, 540, 917
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 1 cut(s) 996
HphI GGTGA 5 cut(s) 517, 539, 549, 828, 1031
Hpy166II GTNNAC 3 cut(s) 530, 729, 736
Hpy188I TCNGA 3 cut(s) 178, 772, 964
Hpy188III TCNNGA 6 cut(s) 94, 116, 139, 458, 914, 1049
Hpy8I GTNNAC 3 cut(s) 530, 729, 736
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 3 cut(s) 466, 931, 1046
HpyCH4III ACNGT 8 cut(s) 170, 422, 589, 678, 692, 733, 828, 1123
HpyCH4IV ACGT 1 cut(s) 907
HpyCH4V TGCA 1 cut(s) 593
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 56, 65, 74, 206, 1012
HpyF3I CTNAG 4 cut(s) 201, 771, 897, 961
HpySE526I ACGT 1 cut(s) 907
Hsp92I GRCGYC 1 cut(s) 656
Hsp92II CATG 6 cut(s) 271, 297, 357, 538, 573, 917
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 7 cut(s) 112, 126, 360, 475, 554, 887, 955
LmnI GCTCC 2 cut(s) 133, 250
Lsp1109I GCAGC 2 cut(s) 68, 1018
LweI GCATC 2 cut(s) 708, 842
MaeI CTAG 3 cut(s) 458, 990, 1049
MaeII ACGT 1 cut(s) 907
MaeIII GTNAC 3 cut(s) 692, 941, 1105
MalI GATC 7 cut(s) 114, 128, 362, 477, 556, 889, 957
MboI GATC 7 cut(s) 112, 126, 360, 475, 554, 887, 955
MboII GAAGA 2 cut(s) 674, 992
MflI RGATCY 2 cut(s) 112, 475
MhlI GDGCHC 3 cut(s) 138, 202, 585
MluCI AATT 7 cut(s) 192, 333, 367, 820, 1032, 1044, 1150
MlyI GAGTC 4 cut(s) 113, 149, 549, 926
MseI TTAA 7 cut(s) 29, 161, 332, 366, 495, 728, 1128
MslI CAYNNNNRTG 1 cut(s) 951
MspI CCGG 1 cut(s) 996
MspR9I CCNGG 4 cut(s) 307, 322, 349, 996
MvaI CCWGG 3 cut(s) 307, 322, 349
MwoI GCNNNNNNNGC 6 cut(s) 41, 56, 65, 74, 206, 1012
NciI CCSGG 1 cut(s) 996
NcoI CCATGG 1 cut(s) 267
NdeII GATC 7 cut(s) 112, 126, 360, 475, 554, 887, 955
NlaIII CATG 6 cut(s) 271, 297, 357, 538, 573, 917
NlaIV GGNNCC 3 cut(s) 231, 273, 477
NmuCI GTSAC 2 cut(s) 692, 1105
NspI RCATGY 1 cut(s) 357
PaeI GCATGC 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 137
PagI TCATGA 1 cut(s) 913
PaqCI CACCTGC 1 cut(s) 856
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 2 cut(s) 58, 1008
PleI GAGTC 4 cut(s) 113, 149, 548, 925
PpsI GAGTC 4 cut(s) 113, 149, 548, 925
PshBI ATTAAT 1 cut(s) 332
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 305, 320, 347
PspEI GGTNACC 1 cut(s) 941
PspGI CCWGG 3 cut(s) 305, 320, 347
PspN4I GGNNCC 3 cut(s) 231, 273, 477
PspPI GGNCC 1 cut(s) 804
PstNI CAGNNNCTG 2 cut(s) 871, 1077
PsuI RGATCY 2 cut(s) 112, 475
RsaI GTAC 4 cut(s) 339, 663, 830, 1070
RsaNI GTAC 4 cut(s) 338, 662, 829, 1069
RseI CAYNNNNRTG 1 cut(s) 951
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 7 cut(s) 29, 161, 332, 366, 495, 728, 1128
SatI GCNGC 2 cut(s) 57, 1007
Sau3AI GATC 7 cut(s) 112, 126, 360, 475, 554, 887, 955
Sau96I GGNCC 1 cut(s) 804
SchI GAGTC 4 cut(s) 113, 149, 549, 926
ScrFI CCNGG 4 cut(s) 307, 322, 349, 996
SduI GDGCHC 3 cut(s) 138, 202, 585
SfaNI GCATC 2 cut(s) 708, 842
SfcI CTRYAG 1 cut(s) 12
Sfr274I CTCGAG 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 951
SmlI CTYRAG 3 cut(s) 137, 149, 395
SmoI CTYRAG 3 cut(s) 137, 149, 395
SphI GCATGC 1 cut(s) 357
Sse9I AATT 7 cut(s) 192, 333, 367, 820, 1032, 1044, 1150
SsiI CCGC 3 cut(s) 66, 1013, 1144
SspMI CTAG 3 cut(s) 458, 990, 1049
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 4 cut(s) 305, 320, 347, 994
StyI CCWWGG 3 cut(s) 108, 267, 501
TaaI ACNGT 8 cut(s) 170, 422, 589, 678, 692, 733, 828, 1123
TaiI ACGT 1 cut(s) 910
TaqI TCGA 5 cut(s) 138, 190, 488, 886, 950
TasI AATT 7 cut(s) 192, 333, 367, 820, 1032, 1044, 1150
TatI WGTACW 1 cut(s) 661
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 7 cut(s) 29, 161, 332, 366, 495, 728, 1128
Tru9I TTAA 7 cut(s) 29, 161, 332, 366, 495, 728, 1128
TscAI CASTG 5 cut(s) 79, 173, 425, 756, 831
TseFI GTSAC 2 cut(s) 692, 1105
TseI GCWGC 2 cut(s) 56, 1006
Tsp45I GTSAC 2 cut(s) 692, 1105
TspDTI ATGAA 1 cut(s) 913
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 5 cut(s) 79, 173, 425, 756, 831
VspI ATTAAT 1 cut(s) 332
XapI RAATTY 2 cut(s) 192, 820
XbaI TCTAGA 2 cut(s) 457, 1048
XceI RCATGY 1 cut(s) 357
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 735
XspI CTAG 3 cut(s) 458, 990, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.