AT4G28820

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010377
Physical Location & Seq
Reverse (-)
14229408 .. 14232380
2973 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28820.4

Sequence Viewer

Length: 522 bp
ATGTGTCCTCGAGCTACTCAAACATGCGAAATCTGCGAGAAGGTTGTATCCAAATACAAGTGTCCTTCTTGTCTTGTTCCTTACTGCTCATTGGGTTGTTTCAAGATACACAAAGAGACTCCATGTGCTAAACCGTCTGATCCTTCTTCAACCGAAGAAAAGCCAGCAGCTTCTCCAGCAAAGGAAGTCCCGGTGAAAAGACCTGAGGAAGCTAATGATGTTGTAGAGAAGACACAGCAAAAGGCTAGTGCTGCATCTCCAGCGAAGGAAATTCCGGTAGCAAGACCGATAATTGTAGAGGAAGAAAAGTATATTTTAGAAAAGACACAGTTCGAGGCTATCGCTTCTTCTAGTGAAATCCGCGAAGCTCTAAAGGATGAACCTCTTCAGAAACTCATCTACAGTATTGATAGTTCTTCCAACCCTTTACAGGAACTCGATGAAGCCATGGGAATAGAAGCATTCCGCGAATTCACAGACAAGATTTTATCAAACATTTCGAAGAGTAATGACGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-HIT

Protein Analysis

173

Amino Acids

19.15

Weight (kDa)

4.97

Isoelectric Point (pI)

65.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 6 - 35 5.4e-11 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010377)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28820 AT4G28820 AT4G28820 AT4G28820 AT4G28820 AT4G28820
fragaria_vesca FvH4_2g19780
malus_domestica MD05G1182200.v1.1 MD10G1170500.v1.1
prunus_persica Prupe.8G209000_v2.0.a1
pyrus_communis pycom05g16760
rosa_chinensis RchiOBHm_Chr6g0285481
rosa_laevigata RLG00000012636
rosa_multiflora Rmu_sc0000284.1_g000002
rosa_roxburghii Rroxscaffold_7G00183110
rosa_rugosa Rorug06G0174800
rosa_samantha Rh6AG286400 Rh6BG287600 Rh6CG288700 Rh6DG281500
rosa_wichuraiana Rw6G024600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 363, 468
AciI CCGC 2 cut(s) 361, 466
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 2 cut(s) 270, 470
AcuI CTGAAG 1 cut(s) 371
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 2 cut(s) 103, 150
AluBI AGCT 4 cut(s) 14, 170, 212, 368
AluI AGCT 4 cut(s) 14, 170, 212, 368
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 9
ApeKI GCWGC 2 cut(s) 167, 251
ApoI RAATTY 2 cut(s) 270, 470
Asp700I GAANNNNTTC 1 cut(s) 384
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 205
AsuII TTCGAA 1 cut(s) 500
AvaI CYCGRG 1 cut(s) 9
AxyI CCTNAGG 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 236
BbvI GCAGC 2 cut(s) 179, 238
BciVI GTATCC 1 cut(s) 58
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 2 cut(s) 246, 351
BfmI CTRYAG 1 cut(s) 400
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 2 cut(s) 168, 252
BlsI GCNGC 2 cut(s) 169, 253
Bme1390I CCNGG 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 236
BpmI CTGGAG 2 cut(s) 159, 243
Bpu14I TTCGAA 1 cut(s) 500
BpuMI CCSGG 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 447
BsaWI WCCGGW 1 cut(s) 274
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse21I CCTNAGG 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 447
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 179, 238
Bsh1236I CGCG 2 cut(s) 363, 468
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 2 cut(s) 191, 275
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 173
BsmI GAATGC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 9
Bsp119I TTCGAA 1 cut(s) 500
Bsp143I GATC 1 cut(s) 139
Bsp19I CCATGG 1 cut(s) 447
BspACI CCGC 2 cut(s) 361, 466
BspCNI CTCAG 1 cut(s) 196
BspFNI CGCG 2 cut(s) 363, 468
BspPI GGATC 1 cut(s) 134
BspT104I TTCGAA 1 cut(s) 500
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 1 cut(s) 139
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 3 cut(s) 135, 330, 404
Bst6I CTCTTC 2 cut(s) 390, 497
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 1 cut(s) 204
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 2 cut(s) 363, 468
BstKTI GATC 1 cut(s) 142
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 139
BstMWI GCNNNNNNNGC 4 cut(s) 33, 176, 251, 260
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 400
BstUI CGCG 2 cut(s) 363, 468
BstV1I GCAGC 2 cut(s) 179, 238
BstV2I GAAGAC 1 cut(s) 236
Bsu36I CCTNAGG 1 cut(s) 204
BsuI GTATCC 1 cut(s) 58
BtgI CCRYGG 1 cut(s) 447
BtsCI GGATG 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 165
CviAII CATG 3 cut(s) 24, 123, 448
CviJI RGCY 8 cut(s) 14, 163, 170, 212, 245, 338, 368, 446
CviKI_1 RGCY 8 cut(s) 14, 163, 170, 212, 245, 338, 368, 446
DdeI CTNAG 1 cut(s) 204
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 390, 497
EarI CTCTTC 2 cut(s) 390, 497
Eco130I CCWWGG 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 371
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 9
EcoRI GAATTC 1 cut(s) 470
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 3 cut(s) 27, 126, 451
FaiI YATR 4 cut(s) 25, 124, 312, 449
FaqI GGGAC 1 cut(s) 173
FatI CATG 3 cut(s) 23, 122, 447
Fnu4HI GCNGC 2 cut(s) 168, 252
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 2 cut(s) 168, 252
FspBI CTAG 2 cut(s) 246, 351
GluI GCNGC 2 cut(s) 168, 252
GsuI CTGGAG 2 cut(s) 159, 243
HapII CCGG 2 cut(s) 191, 275
Hin1II CATG 3 cut(s) 27, 126, 451
HinfI GANTC 1 cut(s) 118
HpaII CCGG 2 cut(s) 191, 275
HphI GGTGA 1 cut(s) 205
Hpy188I TCNGA 2 cut(s) 139, 390
Hpy188III TCNNGA 1 cut(s) 103
HpyAV CCTTC 4 cut(s) 34, 75, 153, 259
HpyCH4III ACNGT 3 cut(s) 135, 330, 404
HpyCH4V TGCA 1 cut(s) 254
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 176, 251, 260
HpyF3I CTNAG 1 cut(s) 204
Hsp92II CATG 3 cut(s) 27, 126, 451
Kzo9I GATC 1 cut(s) 139
LpnPI CCDG 7 cut(s) 177, 189, 204, 216, 273, 288, 416
Lsp1109I GCAGC 2 cut(s) 179, 238
LweI GCATC 1 cut(s) 263
MaeI CTAG 2 cut(s) 246, 351
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 8 cut(s) 138, 167, 241, 314, 339, 377, 408, 514
MluCI AATT 3 cut(s) 270, 291, 470
MlyI GAGTC 1 cut(s) 112
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 5 cut(s) 18, 199, 292, 328, 393
MroXI GAANNNNTTC 1 cut(s) 384
MspI CCGG 2 cut(s) 191, 275
MspR9I CCNGG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 461
MvnI CGCG 2 cut(s) 363, 468
MwoI GCNNNNNNNGC 4 cut(s) 33, 176, 251, 260
NciI CCSGG 1 cut(s) 191
NcoI CCATGG 1 cut(s) 447
NdeII GATC 1 cut(s) 139
NlaIII CATG 3 cut(s) 27, 126, 451
NspI RCATGY 1 cut(s) 27
NspV TTCGAA 1 cut(s) 500
PaeR7I CTCGAG 1 cut(s) 9
PctI GAATGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 384
PkrI GCNGC 2 cut(s) 169, 253
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PspXI VCTCGAGB 1 cut(s) 9
PsrI GAACNNNNNNTAC 2 cut(s) 397, 429
SatI GCNGC 2 cut(s) 168, 252
Sau3AI GATC 1 cut(s) 139
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 1 cut(s) 191
SetI ASST 7 cut(s) 16, 45, 172, 205, 214, 370, 385
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 1 cut(s) 400
Sfr274I CTCGAG 1 cut(s) 9
SfuI TTCGAA 1 cut(s) 500
SlaI CTCGAG 1 cut(s) 9
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
Sse9I AATT 3 cut(s) 270, 291, 470
SsiI CCGC 2 cut(s) 361, 466
SspMI CTAG 2 cut(s) 246, 351
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 3 cut(s) 135, 330, 404
TaqI TCGA 4 cut(s) 10, 333, 438, 500
TaqII GACCGA 1 cut(s) 301
TasI AATT 3 cut(s) 270, 291, 470
TseI GCWGC 2 cut(s) 167, 251
TspDTI ATGAA 2 cut(s) 393, 456
XapI RAATTY 2 cut(s) 270, 470
XceI RCATGY 1 cut(s) 27
XhoI CTCGAG 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 2 cut(s) 246, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.