MD10G1170500.v1.1

Zinc finger HIT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010377
Physical Location & Seq
Forward (+)
26301347 .. 26302796
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1170500.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGGTCATGGAGTAGTGTGCCAAATCTGCAACGAAGCACAATCCAAATACAAGTGCCCCTCCTGCCGCGCACCTTACTGCAGGTCCAGAATTACTGGTAGAGAGGCCGTTAGTTGTGGAGGAACCGCGCGAAGTGCTGCAAAGGCCGCAACTGGAGGCCATAGTGAAATTCGCAGTTCTTTGAAAGATGAGAACCTTCAGAAACTCATTTCGAGTATCGATTGTTCACCAGATGCAGAGAAGGAACTTGAAAAAGCTATGGGCGTGGACGTGTTTCAGATATTTACCGACAAGATCCTATCTACCATCGAGACATCTTCGACTTCAGCAAACCTCGGTGCAAACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-HIT

Protein Analysis

117

Amino Acids

12.29

Weight (kDa)

7.55

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 6 - 27 4.2e-07 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010377)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28820 AT4G28820 AT4G28820 AT4G28820 AT4G28820 AT4G28820
fragaria_vesca FvH4_2g19780
malus_domestica MD05G1182200.v1.1 MD10G1170500.v1.1
prunus_persica Prupe.8G209000_v2.0.a1
pyrus_communis pycom05g16760
rosa_chinensis RchiOBHm_Chr6g0285481
rosa_laevigata RLG00000012636
rosa_multiflora Rmu_sc0000284.1_g000002
rosa_roxburghii Rroxscaffold_7G00183110
rosa_rugosa Rorug06G0174800
rosa_samantha Rh6AG286400 Rh6BG287600 Rh6CG288700 Rh6DG281500
rosa_wichuraiana Rw6G024600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 72
AccII CGCG 3 cut(s) 69, 128, 130
AciI CCGC 3 cut(s) 67, 126, 147
AclWI GGATC 1 cut(s) 289
AcsI RAATTY 1 cut(s) 168
AcuI CTGAAG 2 cut(s) 182, 309
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 2 cut(s) 184, 251
AjiI CACGTC 1 cut(s) 271
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 1 cut(s) 289
AoxI GGCC 3 cut(s) 105, 144, 157
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 1 cut(s) 168
AspLEI GCGC 2 cut(s) 71, 130
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 219
AvaII GGWCC 1 cut(s) 84
BaeGI GKGCMC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 124
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 305
BfmI CTRYAG 1 cut(s) 79
BfuAI ACCTGC 1 cut(s) 72
BisI GCNGC 3 cut(s) 67, 138, 147
BlsI GCNGC 3 cut(s) 68, 139, 148
Bme18I GGWCC 1 cut(s) 84
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 124
BmsI GCATC 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 174
Bsa29I ATCGAT 1 cut(s) 219
BsaJI CCNNGG 1 cut(s) 334
Bse1I ACTGG 2 cut(s) 100, 157
BseCI ATCGAT 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 334
BseNI ACTGG 2 cut(s) 100, 157
BseSI GKGCMC 1 cut(s) 59
BseXI GCAGC 1 cut(s) 124
Bsh1236I CGCG 3 cut(s) 69, 128, 130
BshFI GGCC 3 cut(s) 107, 146, 159
BshVI ATCGAT 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 305
BsnI GGCC 3 cut(s) 107, 146, 159
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 3 cut(s) 67, 126, 147
BspANI GGCC 3 cut(s) 107, 146, 159
BspDI ATCGAT 1 cut(s) 219
BspFNI CGCG 3 cut(s) 69, 128, 130
BspLI GGNNCC 1 cut(s) 124
BspMAI CTGCAG 1 cut(s) 83
BspMI ACCTGC 1 cut(s) 72
BspPI GGATC 1 cut(s) 289
BsrI ACTGG 2 cut(s) 100, 157
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 1 cut(s) 294
BstFNI CGCG 3 cut(s) 69, 128, 130
BstHHI GCGC 2 cut(s) 71, 130
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 5 cut(s) 27, 63, 134, 143, 146
BstSFI CTRYAG 1 cut(s) 79
BstSLI GKGCMC 1 cut(s) 59
BstUI CGCG 3 cut(s) 69, 128, 130
BstV1I GCAGC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
Bsu15I ATCGAT 1 cut(s) 219
BsuRI GGCC 3 cut(s) 107, 146, 159
BsuTUI ATCGAT 1 cut(s) 219
BtrI CACGTC 1 cut(s) 271
BveI ACCTGC 1 cut(s) 72
CfoI GCGC 2 cut(s) 71, 130
Cfr13I GGNCC 1 cut(s) 84
ClaI ATCGAT 1 cut(s) 219
CviAII CATG 1 cut(s) 8
CviJI RGCY 4 cut(s) 107, 146, 159, 257
CviKI_1 RGCY 4 cut(s) 107, 146, 159, 257
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 84
Eco57I CTGAAG 2 cut(s) 182, 309
FaeI CATG 1 cut(s) 11
FaiI YATR 4 cut(s) 9, 162, 260, 349
FatI CATG 1 cut(s) 7
Fnu4HI GCNGC 3 cut(s) 67, 138, 147
Fsp4HI GCNGC 3 cut(s) 67, 138, 147
GlaI GCGC 2 cut(s) 70, 129
GluI GCNGC 3 cut(s) 67, 138, 147
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 3 cut(s) 107, 146, 159
HhaI GCGC 2 cut(s) 71, 130
Hin1II CATG 1 cut(s) 11
Hin6I GCGC 2 cut(s) 69, 128
HinP1I GCGC 2 cut(s) 69, 128
HphI GGTGA 1 cut(s) 219
Hpy166II GTNNAC 2 cut(s) 227, 268
Hpy188I TCNGA 2 cut(s) 201, 279
Hpy188III TCNNGA 2 cut(s) 87, 310
Hpy8I GTNNAC 2 cut(s) 227, 268
HpyAV CCTTC 2 cut(s) 206, 235
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 5 cut(s) 30, 81, 140, 236, 341
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 63, 134, 143, 146
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 1 cut(s) 11
HspAI GCGC 2 cut(s) 69, 128
Kzo9I GATC 1 cut(s) 294
LpnPI CCDG 6 cut(s) 67, 76, 81, 100, 138, 243
Lsp1109I GCAGC 1 cut(s) 124
LweI GCATC 1 cut(s) 223
MaeII ACGT 1 cut(s) 270
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 1 cut(s) 309
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 2 cut(s) 90, 168
MnlI CCTC 5 cut(s) 70, 97, 113, 149, 344
MvnI CGCG 3 cut(s) 69, 128, 130
MwoI GCNNNNNNNGC 5 cut(s) 27, 63, 134, 143, 146
NdeII GATC 1 cut(s) 294
NlaIII CATG 1 cut(s) 11
NlaIV GGNNCC 1 cut(s) 124
PkrI GCNGC 3 cut(s) 68, 139, 148
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 1 cut(s) 84
PsrI GAACNNNNNNTAC 2 cut(s) 208, 240
PstI CTGCAG 1 cut(s) 83
PsuI RGATCY 1 cut(s) 294
SatI GCNGC 3 cut(s) 67, 138, 147
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 1 cut(s) 84
SduI GDGCHC 1 cut(s) 59
SetI ASST 6 cut(s) 76, 86, 198, 259, 273, 336
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 1 cut(s) 79
SinI GGWCC 1 cut(s) 84
Sse9I AATT 2 cut(s) 90, 168
SsiI CCGC 3 cut(s) 67, 126, 147
TaiI ACGT 1 cut(s) 273
TaqI TCGA 4 cut(s) 212, 219, 309, 320
TasI AATT 2 cut(s) 90, 168
TauI GCSGC 2 cut(s) 69, 149
TseI GCWGC 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.