MD05G1182200.v1.1

Zinc finger HIT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010377
Physical Location & Seq
Forward (+)
31072888 .. 31075241
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1182200.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGGTCCTCGAGTAGTGTGCCAAGTCTGCAACGAAGCACAATCCAAATACAAGTGCCCCTCCTGCCGCGCACCTTATTGTTCTGTGCCCTGCTTCAAGAAGCACAAGGAAAACCCATGTTCTCTTCCAGTTTCTTCCGAGGAAAAACCACCAACTGCAGGTCCAGAGCTACTGGTAGAGAGGCCATTACTTGTGGAGGAACCGAGCCAAGTGCTGCAAAGGCCGCAACTTGAGGCGATAGCTTCTTCCGGTGAAATTTGCAGTGCTTTGAAAGATGAGAACCTTCAGAAACTTATCTTGAGTATAGATTGTTCACCAGATGCAGAGAAGGAACTTGAGAAAGCTATGGGCGTGGACGTGTTTCGCGTATTTACCGACAAGATCCTATCTACCATCGAGACGTCTTCGTCTTCAGCAAATGCAAACTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-HIT

Protein Analysis

144

Amino Acids

15.57

Weight (kDa)

5.11

Isoelectric Point (pI)

53.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 3 - 33 2.7e-11 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010377)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28820 AT4G28820 AT4G28820 AT4G28820 AT4G28820 AT4G28820
fragaria_vesca FvH4_2g19780
malus_domestica MD05G1182200.v1.1 MD10G1170500.v1.1
prunus_persica Prupe.8G209000_v2.0.a1
pyrus_communis pycom05g16760
rosa_chinensis RchiOBHm_Chr6g0285481
rosa_laevigata RLG00000012636
rosa_multiflora Rmu_sc0000284.1_g000002
rosa_roxburghii Rroxscaffold_7G00183110
rosa_rugosa Rorug06G0174800
rosa_samantha Rh6AG286400 Rh6BG287600 Rh6CG288700 Rh6DG281500
rosa_wichuraiana Rw6G024600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 406
AatII GACGTC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 149
AccII CGCG 2 cut(s) 69, 366
AciI CCGC 2 cut(s) 67, 224
AclWI GGATC 1 cut(s) 376
AcsI RAATTY 1 cut(s) 255
AcuI CTGAAG 2 cut(s) 269, 396
AcyI GRCGYC 1 cut(s) 401
AfiI CCNNNNNNNGG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 2 cut(s) 97, 271
AjiI CACGTC 1 cut(s) 358
AluBI AGCT 3 cut(s) 169, 242, 344
AluI AGCT 3 cut(s) 169, 242, 344
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 1 cut(s) 376
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 2 cut(s) 182, 221
ApeKI GCWGC 1 cut(s) 214
ApoI RAATTY 1 cut(s) 255
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 2 cut(s) 5, 161
AsuHPI GGTGA 2 cut(s) 263, 306
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 2 cut(s) 5, 161
BaeGI GKGCMC 2 cut(s) 59, 90
BbsI GAAGAC 2 cut(s) 396, 402
BbvI GCAGC 1 cut(s) 201
BccI CCATC 1 cut(s) 401
BcgI CGANNNNNNTGC 3 cut(s) 34, 193, 227
BcoDI GTCTC 1 cut(s) 392
BfmI CTRYAG 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 149
BisI GCNGC 3 cut(s) 67, 215, 224
BlsI GCNGC 3 cut(s) 68, 216, 225
Bme18I GGWCC 2 cut(s) 5, 161
BmeT110I CYCGRG 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 5, 161
BmiI GGNNCC 2 cut(s) 6, 201
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 2 cut(s) 396, 402
BpuEI CTTGAG 3 cut(s) 251, 319, 356
BsaHI GRCGYC 1 cut(s) 401
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 1 cut(s) 248
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 128, 177
BseDI CCNNGG 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 128, 177
BseSI GKGCMC 2 cut(s) 59, 90
BseXI GCAGC 1 cut(s) 201
Bsh1236I CGCG 2 cut(s) 69, 366
BshFI GGCC 2 cut(s) 184, 223
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 249
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 1 cut(s) 392
BsmBI CGTCTC 1 cut(s) 392
BsnI GGCC 2 cut(s) 184, 223
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 59, 90
Bsp143I GATC 1 cut(s) 381
BspACI CCGC 2 cut(s) 67, 224
BspANI GGCC 2 cut(s) 184, 223
BspFNI CGCG 2 cut(s) 69, 366
BspHI TCATGA 1 cut(s) 428
BspLI GGNNCC 2 cut(s) 6, 201
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 1 cut(s) 376
BsrI ACTGG 2 cut(s) 128, 177
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 1 cut(s) 381
BssNI GRCGYC 1 cut(s) 401
Bst6I CTCTTC 1 cut(s) 129
BstACI GRCGYC 1 cut(s) 401
BstFNI CGCG 2 cut(s) 69, 366
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 1 cut(s) 384
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 1 cut(s) 381
BstMWI GCNNNNNNNGC 4 cut(s) 27, 63, 220, 223
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 2 cut(s) 59, 90
BstUI CGCG 2 cut(s) 69, 366
BstV1I GCAGC 1 cut(s) 201
BstV2I GAAGAC 2 cut(s) 396, 402
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BsuRI GGCC 2 cut(s) 184, 223
BtrI CACGTC 1 cut(s) 358
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 268
BveI ACCTGC 1 cut(s) 149
CciI TCATGA 1 cut(s) 428
CfoI GCGC 1 cut(s) 71
Cfr13I GGNCC 2 cut(s) 5, 161
CviAII CATG 2 cut(s) 117, 429
CviJI RGCY 6 cut(s) 169, 184, 207, 223, 242, 344
CviKI_1 RGCY 6 cut(s) 169, 184, 207, 223, 242, 344
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
DrdI GACNNNNNNGTC 1 cut(s) 406
DseDI GACNNNNNNGTC 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 5, 161
Eco57I CTGAAG 2 cut(s) 269, 396
Eco88I CYCGRG 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 5
Esp3I CGTCTC 1 cut(s) 392
FaeI CATG 2 cut(s) 120, 432
FaiI YATR 4 cut(s) 118, 305, 347, 430
FatI CATG 2 cut(s) 116, 428
Fnu4HI GCNGC 3 cut(s) 67, 215, 224
Fsp4HI GCNGC 3 cut(s) 67, 215, 224
GlaI GCGC 1 cut(s) 70
GluI GCNGC 3 cut(s) 67, 215, 224
HaeIII GGCC 2 cut(s) 184, 223
HapII CCGG 1 cut(s) 249
HhaI GCGC 1 cut(s) 71
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 2 cut(s) 120, 432
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HpaII CCGG 1 cut(s) 249
HphI GGTGA 2 cut(s) 263, 306
Hpy166II GTNNAC 2 cut(s) 314, 355
Hpy188I TCNGA 2 cut(s) 139, 288
Hpy188III TCNNGA 5 cut(s) 97, 164, 298, 397, 429
Hpy8I GTNNAC 2 cut(s) 314, 355
HpyAV CCTTC 2 cut(s) 293, 322
HpyCH4IV ACGT 2 cut(s) 357, 401
HpyCH4V TGCA 6 cut(s) 30, 158, 217, 261, 323, 422
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 63, 220, 223
HpySE526I ACGT 2 cut(s) 357, 401
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 2 cut(s) 120, 432
HspAI GCGC 1 cut(s) 69
Kzo9I GATC 1 cut(s) 381
LpnPI CCDG 8 cut(s) 76, 103, 141, 144, 158, 177, 262, 330
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 1 cut(s) 310
MaeII ACGT 2 cut(s) 357, 401
MalI GATC 1 cut(s) 383
MboI GATC 1 cut(s) 381
MboII GAAGA 5 cut(s) 116, 126, 237, 396, 402
MflI RGATCY 1 cut(s) 381
MhlI GDGCHC 2 cut(s) 59, 90
MluCI AATT 1 cut(s) 255
MnlI CCTC 6 cut(s) 18, 70, 133, 174, 190, 226
MspI CCGG 1 cut(s) 249
MvnI CGCG 2 cut(s) 69, 366
MwoI GCNNNNNNNGC 4 cut(s) 27, 63, 220, 223
NdeII GATC 1 cut(s) 381
NlaIII CATG 2 cut(s) 120, 432
NlaIV GGNNCC 2 cut(s) 6, 201
PaeR7I CTCGAG 1 cut(s) 9
PagI TCATGA 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PkrI GCNGC 3 cut(s) 68, 216, 225
PpuMI RGGWCCY 1 cut(s) 5
Psp5II RGGWCCY 1 cut(s) 5
PspN4I GGNNCC 2 cut(s) 6, 201
PspPI GGNCC 2 cut(s) 5, 161
PspPPI RGGWCCY 1 cut(s) 5
PspXI VCTCGAGB 1 cut(s) 9
PsrI GAACNNNNNNTAC 2 cut(s) 295, 327
PstI CTGCAG 1 cut(s) 160
PsuI RGATCY 1 cut(s) 381
SatI GCNGC 3 cut(s) 67, 215, 224
Sau3AI GATC 1 cut(s) 381
Sau96I GGNCC 2 cut(s) 5, 161
SduI GDGCHC 2 cut(s) 59, 90
SetI ASST 8 cut(s) 76, 163, 171, 244, 285, 346, 360, 404
SfaNI GCATC 1 cut(s) 310
SfcI CTRYAG 1 cut(s) 156
Sfr274I CTCGAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 5, 161
SlaI CTCGAG 1 cut(s) 9
SmlI CTYRAG 4 cut(s) 9, 230, 298, 335
SmoI CTYRAG 4 cut(s) 9, 230, 298, 335
Sse9I AATT 1 cut(s) 255
SsiI CCGC 2 cut(s) 67, 224
TaiI ACGT 2 cut(s) 360, 404
TaqI TCGA 2 cut(s) 10, 396
TasI AATT 1 cut(s) 255
TauI GCSGC 2 cut(s) 69, 226
TscAI CASTG 1 cut(s) 268
TseI GCWGC 1 cut(s) 214
TspRI CASTG 1 cut(s) 268
VpaK11BI GGWCC 2 cut(s) 5, 161
XapI RAATTY 1 cut(s) 255
XhoI CTCGAG 1 cut(s) 9
ZraI GACGTC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.