Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0013189

Physical Location & Seq

Genomic Coordinates Forward (+)

15009471 .. 15012588

Gene Structure 3118 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G30825.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2715 bp

ATGGGTTCGTTAAGGTTTTCCATTCCATTGGACCCATTTGACTCAAAAAGAAAAAGGTTTCATTTTTCTGCAAACCCTTCTCAGTTTCCTGACCAATTTCCAATTCACTTTGTTACCAGTAGTATTCACGCCACTAGAGCTTCCTCAATCGGTTCTTCGACTAGGGTGCTTGACAAAATTCGAGTCTCTAGTCTCGGTACTGAAGCAAACGAGAATGCTATTAATTCTGCAAGTGCAGCTCCTGTGGAGAGAAGCCGGAGTTCTAAATTGAGTGGTGATCAGAGAGGGACAAAGAAATATGTAGCTCGGAAGTTCAGTTTCCGAAGAGGAAGCAATGATCTTGAGCTTGAGAATTTGTTTGTGAACAATGGGGAAATAGATGTTAACTATTCCGCTATTAAGCCCGGTCAGAGTTTGGAGCATTGCAATGGAATATTGAAACGGCTTGAGAGTTGCAGTGACACGAATGCCATCAAATTCTTTGATTGGATGAGATGTAATGGTAAATTGGTAGGTAACTTTGTTGCTTATAGTTTGATTCTCAGAGTTTTAGGAAGGAGAGAAGAATGGGACCGAGCCGAGGATCTGATCAAGGAGCTGTGTGGATTTCATGAGTTTCAGAAGAGTTATCAGGTCTTCAACACGGTTATATATGCATGTACTAAAAAGGGAAATGTGAAATTAGCTTCAAAGTGGTTTCACATGATGTTGGAGTTTGGTGTTAGACCAAATGTGGCTACAATAGGTATGCTCATGGGTCTTTACCAGAAGAACTGGAATGTTGAAGAAGCGGAATTTGCATTTTCTCATATGAGAAAATTCGGGATTGTGTGTGAATCTGCTTACTCCTCGATGATAACGATTTACACGCGTTTGAGATTATATGATAAAGCAGAGGAAGTTATAGACTTGATGAAACAAGATAGAGTGAGGCTGAAGTTAGAGAATTGGTTAGTGATGCTTAATGCTTATAGTCAACAAGGAAAAATGGAGCTAGCTGAATCTATATTGGTTTCTATGGAAGCTGCGGGGTTTTCTCCGAATATCATTGCATACAATACTCTGATAACTGGTTATGGTAAGATTTTTAAGATGGAGGCTGCACAGGGTTTGTTCCACCGCCTCTGTAATATTGGGCTGGAACCAGATGAGACAAGTTACAGGTCAATGATTGAAGGTTGGGGCCGCGCTGACAATTACGAGGAGGCAAAACATTACTATCAAGAGCTGAAGCGGTGTGGTTATAAACCCAATTCATTCAATCTTTTTACTTTAATCAATTTACAGGCTAAATATGGAGACAGAGATGGTGCTATTAAGACAATCGAAGATATGACTGGTATCGGGTGCCAATATTCCTCGATTCTTGGCATCATTCTGCAGGCTTATGAGAAGGTGGGGAAGATTGATGTGGTGCCTTGCGTTCTCAAAGGTTCTTTTCATAATCATATCCGTTTAAACCAGACTTCTTTCTCGAGTCTAGTGATGGCATATGTCAAACATGGTATGGTTGATGATTGCTTGGGGTTGTTGCGAGAAAAAAAGTGGAGAGACTCAGCATTTGAGTCCCACTTGTATCATCTGTTGATTTGCTCTTGCAAAGAATCTGGTCAACTTACTGATGCTGTGAAAATATACAACCATAAAATGGAATCTGATGAAGAGATAAATCTGCACATTACGTCTACAATGATTGACATTTATACTGTGATGGGTGAGTTTAGTGAAGCGGAGAAGCTTTATTTAAATTTGAAATCTTCTGGAGTTGTGTTGGACAGGATTGGATTCAGTATTGTGGTGCGGATGTATGTGAAAGCCGGATCCTTGGAAGAAGCCTGTTCTGTCCTAGAAATCATGGATGAGCAGAAAGACATTGTTCCGGATGTGTACTTGTTCCGCGATATGCTTCGGATATATCAGAAGTGTGACCTTCAGGATAAACTCCAGCATCTGTACTACAGGATTCGAAAGAGTGGGATCCATTGGAATCAGGAGATGTACAATTGTGTTATAAACTGCTGCGCACGTGCTCTTCCGCTTGATGAACTCTCAGGAACCTTTGAGGAAATGATTCGATATGGATTCACTCCTAATACTGTCACATTCAATGTCCTCCTTGATGTATATGGTAAAGCCAAGCTCTTCAAAAAGGTTAACGAGCTTTTCTTGTTGGCGAAAAGACACGGAGTTGTGGATGTTATATCCTACAACACCATTATAGCTGCCTATGGAAAGAACAAGGACTATACAAACATGTCATCAGCGATTAAGAATATGCAATTTGACGGGTTTTCTGTGTCACTTGAAGCTTATAATACTTTGTTGGATGCATATGGGAAAGATAAACAGATGGAGAAATTCAGAAGCATCTTGAAAAGAATGAAGAAGTCGACTAGTGGACCGGATCATTATACATACAACATCATGATCAATATATATGGAGAGCAAGGATGGATCGATGAAGTCGCTGATGTTCTGAAAGAATTGAAAGAATCGGGACTTGGTCCTGACTTGTGTAGCTATAACACGCTGATAAAGGCGTATGGTATAGGTGGAATGGTTGAAGAAGCTGTTGGGTTGGTGAAGGAGATGAGGGGAAGGAACATAATACCTGATAAAGTAACTTACACCAATTTGGTCACAGCTTTGCGCAGGAATGATGAATTTCTTGAGGCTATTAAATGGTCTTTGTGGATGAAACAGATGGGTATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000079 GO:0000428 GO:0001932 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005675 GO:0006139 GO:0006259 GO:0006281 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016591 GO:0019219 GO:0019220 GO:0019222 GO:0030880 GO:0031323 GO:0031326 GO:0031399 GO:0031974 GO:0031981 GO:0032268 GO:0032806 GO:0032991 GO:0033554 GO:0034641 GO:0042325 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043549 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045859 GO:0046483 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051174 GO:0051246 GO:0051252 GO:0051338 GO:0051716 GO:0051726 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0071900 GO:0080090 GO:0090304 GO:0090575 GO:1901360 GO:1902494 GO:1902554 GO:1902911 GO:1903506 GO:1904029 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

904

Amino Acids

103.31

Weight (kDa)

8.28

Isoelectric Point (pI)

34.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	212 - 252	8.8e-07	PPR repeat family
PPR_2	PF13041	319 - 360	5.2e-08	PPR repeat family
PPR_3	PF13812	319 - 361	1.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	347 - 393	1.4e-09	PPR repeat family
PPR_3	PF13812	656 - 711	3.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	666 - 709	1.9e-07	PPR repeat family
PPR_long	PF17177	744 - 880	3e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	757 - 815	1e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	770 - 811	2.2e-10	PPR repeat family
PPR_2	PF13041	801 - 848	1.6e-09	PPR repeat family
PPR	PF01535	804 - 834	6.3e-06	PPR repeat
PPR_1	PF12854	833 - 865	6.8e-07	PPR repeat
PPR_2	PF13041	836 - 883	1e-11	PPR repeat family
PPR	PF01535	840 - 869	3.8e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013189)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30825
fragaria_vesca	FvH4_1g00580
malus_domestica	MD15G1148600.v1.1
prunus_persica	Prupe.7G266300_v2.0.a1
pyrus_communis	pycom15g13290 pycom15g13300
rosa_chinensis	RchiOBHm_Chr2g0085211
rosa_laevigata	RLG00000015667
rosa_multiflora	Rmu_sc0008510.1_g000001 Rmu_sc0008647.1_g000001 Rmu_sc0011989.1_g000006
rosa_roxburghii	Rroxscaffold_2G00155560
rosa_rugosa	Rorug01G0458400
rosa_samantha	Rh2AG007000 Rh2BG007600 Rh2CG008400 Rh2DG008700
rosa_wichuraiana	Rw2G000650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1245, 2012, 2313
Acc16I	TGCGCA	2 cut(s)	2023, 2651
AccB1I	GGYRCC	2 cut(s)	1347, 1414
AccB7I	CCANNNNNTGG	1 cut(s)	1648
AccI	GTMKAC	2 cut(s)	1685, 2390
AccII	CGCG	3 cut(s)	871, 1188, 1899
AccIII	TCCGGA	1 cut(s)	1879
AclWI	GGATC	7 cut(s)	591, 1815, 1828, 1972, 1985, 2412, 2462
AcsI	RAATTY	8 cut(s)	177, 352, 476, 794, 818, 1747, 2357, 2663
AcuI	CTGAAG	4 cut(s)	222, 956, 1250, 1916
AcvI	CACGTG	1 cut(s)	2027
AfaI	GTAC	5 cut(s)	199, 661, 1889, 1955, 2000
AfiI	CCNNNNNNNGG	2 cut(s)	580, 1648
AflIII	ACRYGT	2 cut(s)	869, 2253
AhlI	ACTAGT	1 cut(s)	2393
AjuI	GAANNNNNNNTTGG	6 cut(s)	87, 119, 2484, 2516, 2556, 2588
Alw21I	GWGCWC	1 cut(s)	2032
Alw26I	GTCTC	5 cut(s)	190, 197, 1145, 1293, 1545
AlwI	GGATC	7 cut(s)	591, 1815, 1828, 1972, 1985, 2412, 2462
AlwNI	CAGNNNCTG	2 cut(s)	242, 1951
Ama87I	CYCGRG	1 cut(s)	1474
Aor13HI	TCCGGA	1 cut(s)	1879
AoxI	GGCC	1 cut(s)	1183
ApeKI	GCWGC	5 cut(s)	236, 1025, 1100, 2019, 2222
ApoI	RAATTY	8 cut(s)	177, 352, 476, 794, 818, 1747, 2357, 2663
ArsI	GACNNNNNNTTYG	4 cut(s)	168, 200, 1544, 1576
AseI	ATTAAT	1 cut(s)	222
Asp700I	GAANNNNTTC	1 cut(s)	2070
AspLEI	GCGC	3 cut(s)	1190, 2024, 2652
AspS9I	GGNCC	5 cut(s)	31, 571, 1183, 2399, 2504
AsuC2I	CCSGG	1 cut(s)	405
AsuHPI	GGTGA	3 cut(s)	287, 1727, 2593
AsuII	TTCGAA	1 cut(s)	1966
AsuNHI	GCTAGC	1 cut(s)	994
AvaI	CYCGRG	1 cut(s)	1474
AvaII	GGWCC	4 cut(s)	31, 571, 2399, 2504
BamHI	GGATCC	2 cut(s)	1820, 1977
BanI	GGYRCC	2 cut(s)	1347, 1414
BbrPI	CACGTG	1 cut(s)	2027
BbsI	GAAGAC	1 cut(s)	628
Bbv12I	GWGCWC	1 cut(s)	2032
BbvI	GCAGC	5 cut(s)	248, 1012, 1087, 2006, 2209
BccI	CCATC	8 cut(s)	479, 1087, 1301, 1480, 1705, 2344, 2445, 2698
BceAI	ACGGC	1 cut(s)	458
BcgI	CGANNNNNNTGC	2 cut(s)	148, 182
BclI	TGATCA	3 cut(s)	277, 588, 2427
BcnI	CCSGG	1 cut(s)	405
BcoDI	GTCTC	5 cut(s)	190, 197, 1145, 1293, 1545
BcuI	ACTAGT	1 cut(s)	2393
BfaI	CTAG	7 cut(s)	135, 162, 189, 995, 1481, 1847, 2394
BfmI	CTRYAG	2 cut(s)	1379, 1957
BisI	GCNGC	6 cut(s)	237, 1026, 1101, 1186, 2020, 2223
BlsI	GCNGC	6 cut(s)	238, 1027, 1102, 1187, 2021, 2224
Bme1390I	CCNGG	1 cut(s)	405
Bme18I	GGWCC	4 cut(s)	31, 571, 2399, 2504
BmeT110I	CYCGRG	1 cut(s)	1474
BmgT120I	GGNCC	5 cut(s)	31, 571, 1183, 2399, 2504
BmiI	GGNNCC	9 cut(s)	33, 572, 1143, 1184, 1349, 1416, 1822, 1979, 2056
BmrFI	CCNGG	1 cut(s)	405
BmsI	GCATC	6 cut(s)	948, 1380, 1612, 1957, 2317, 2376
BmtI	GCTAGC	1 cut(s)	998
BpiI	GAAGAC	1 cut(s)	628
BpmI	CTGGAG	2 cut(s)	1782, 1928
Bpu14I	TTCGAA	1 cut(s)	1966
BpuEI	CTTGAG	4 cut(s)	362, 368, 467, 2690
BpuMI	CCSGG	1 cut(s)	405
Bsa29I	ATCGAT	1 cut(s)	2457
BsaAI	YACGTR	1 cut(s)	2027
BsaJI	CCNNGG	2 cut(s)	579, 1824
BsaWI	WCCGGW	2 cut(s)	1879, 2401
Bsc4I	CCNNNNNNNGG	2 cut(s)	580, 1648
Bse1I	ACTGG	4 cut(s)	117, 779, 1075, 1342
Bse3DI	GCAATG	4 cut(s)	340, 421, 433, 1047
BseAI	TCCGGA	1 cut(s)	1879
BseCI	ATCGAT	1 cut(s)	2457
BseDI	CCNNGG	2 cut(s)	579, 1824
BseGI	GGATG	8 cut(s)	495, 1809, 1864, 1888, 2200, 2332, 2456, 2700
BseLI	CCNNNNNNNGG	2 cut(s)	580, 1648
BseMI	GCAATG	4 cut(s)	340, 421, 433, 1047
BseMII	CTCAG	4 cut(s)	95, 556, 1569, 2064
BseNI	ACTGG	4 cut(s)	117, 779, 1075, 1342
BseRI	GAGGAG	2 cut(s)	838, 1217
BseXI	GCAGC	5 cut(s)	248, 1012, 1087, 2006, 2209
BsgI	GTGCAG	3 cut(s)	255, 1086, 1658
Bsh1236I	CGCG	3 cut(s)	871, 1188, 1899
BshFI	GGCC	1 cut(s)	1185
BshNI	GGYRCC	2 cut(s)	1347, 1414
BshVI	ATCGAT	1 cut(s)	2457
BsiHKAI	GWGCWC	1 cut(s)	2032
BsiHKCI	CYCGRG	1 cut(s)	1474
BsiSI	CCGG	5 cut(s)	256, 405, 1818, 1880, 2402
BslFI	GGGAC	4 cut(s)	301, 584, 1552, 2511
BslI	CCNNNNNNNGG	2 cut(s)	580, 1648
BsmAI	GTCTC	5 cut(s)	190, 197, 1145, 1293, 1545
BsmFI	GGGAC	4 cut(s)	301, 584, 1552, 2511
BsmI	GAATGC	2 cut(s)	220, 472
BsnI	GGCC	1 cut(s)	1185
BsoBI	CYCGRG	1 cut(s)	1474
Bsp119I	TTCGAA	1 cut(s)	1966
Bsp1286I	GDGCHC	1 cut(s)	2032
Bsp13I	TCCGGA	1 cut(s)	1879
Bsp1407I	TGTACA	1 cut(s)	1998
Bsp143I	GATC	9 cut(s)	277, 337, 583, 588, 1820, 1977, 2404, 2427, 2454
BspANI	GGCC	1 cut(s)	1185
BspCNI	CTCAG	4 cut(s)	94, 555, 1568, 2063
BspDI	ATCGAT	1 cut(s)	2457
BspEI	TCCGGA	1 cut(s)	1879
BspFNI	CGCG	3 cut(s)	871, 1188, 1899
BspHI	TCATGA	2 cut(s)	610, 2424
BspLI	GGNNCC	9 cut(s)	33, 572, 1143, 1184, 1349, 1416, 1822, 1979, 2056
BspMAI	CTGCAG	1 cut(s)	1383
BspOI	GCTAGC	1 cut(s)	998
BspPI	GGATC	7 cut(s)	591, 1815, 1828, 1972, 1985, 2412, 2462
BspQI	GCTCTTC	2 cut(s)	2037, 2147
BspT104I	TTCGAA	1 cut(s)	1966
BspT107I	GGYRCC	2 cut(s)	1347, 1414
BsrDI	GCAATG	4 cut(s)	340, 421, 433, 1047
BsrGI	TGTACA	1 cut(s)	1998
BsrI	ACTGG	4 cut(s)	117, 779, 1075, 1342
BssECI	CCNNGG	2 cut(s)	579, 1824
BssMI	GATC	9 cut(s)	277, 337, 583, 588, 1820, 1977, 2404, 2427, 2454
BssT1I	CCWWGG	1 cut(s)	1824
Bst4CI	ACNGT	3 cut(s)	646, 1708, 2098
Bst6I	CTCTTC	5 cut(s)	319, 617, 1656, 2037, 2147
BstAUI	TGTACA	1 cut(s)	1998
BstBAI	YACGTR	1 cut(s)	2027
BstBI	TTCGAA	1 cut(s)	1966
BstC8I	GCNNGC	2 cut(s)	996, 1383
BstDEI	CTNAG	4 cut(s)	81, 542, 1555, 2050
BstF5I	GGATG	8 cut(s)	495, 1809, 1864, 1888, 2200, 2332, 2456, 2700
BstFNI	CGCG	3 cut(s)	871, 1188, 1899
BstHHI	GCGC	3 cut(s)	1190, 2024, 2652
BstKTI	GATC	9 cut(s)	280, 340, 586, 591, 1823, 1980, 2407, 2430, 2457
BstMAI	GTCTC	5 cut(s)	190, 197, 1145, 1293, 1545
BstMBI	GATC	9 cut(s)	277, 337, 583, 588, 1820, 1977, 2404, 2427, 2454
BstMWI	GCNNNNNNNGC	3 cut(s)	137, 236, 797
BstNSI	RCATGY	2 cut(s)	660, 2257
BstSCI	CCNGG	1 cut(s)	403
BstSFI	CTRYAG	2 cut(s)	1379, 1957
BstUI	CGCG	3 cut(s)	871, 1188, 1899
BstV1I	GCAGC	5 cut(s)	248, 1012, 1087, 2006, 2209
BstV2I	GAAGAC	1 cut(s)	628
BstX2I	RGATCY	3 cut(s)	583, 1820, 1977
BstXI	CCANNNNNNTGG	1 cut(s)	28
BstYI	RGATCY	3 cut(s)	583, 1820, 1977
Bsu15I	ATCGAT	1 cut(s)	2457
BsuRI	GGCC	1 cut(s)	1185
BsuTUI	ATCGAT	1 cut(s)	2457
BtsCI	GGATG	8 cut(s)	495, 1809, 1864, 1888, 2200, 2332, 2456, 2700
BtsI	GCAGTG	1 cut(s)	463
BtsIMutI	CAGTG	1 cut(s)	463
Cac8I	GCNNGC	2 cut(s)	996, 1383
CaiI	CAGNNNCTG	2 cut(s)	242, 1951
CciI	TCATGA	2 cut(s)	610, 2424
CfoI	GCGC	3 cut(s)	1190, 2024, 2652
Cfr13I	GGNCC	5 cut(s)	31, 571, 1183, 2399, 2504
ClaI	ATCGAT	1 cut(s)	2457
Csp6I	GTAC	5 cut(s)	198, 660, 1888, 1954, 1999
CviAII	CATG	8 cut(s)	611, 657, 703, 754, 1502, 1855, 2254, 2425
CviQI	GTAC	5 cut(s)	198, 660, 1888, 1954, 1999
DdeI	CTNAG	4 cut(s)	81, 542, 1555, 2050
DpnI	GATC	9 cut(s)	279, 339, 585, 590, 1822, 1979, 2406, 2429, 2456
DpnII	GATC	9 cut(s)	277, 337, 583, 588, 1820, 1977, 2404, 2427, 2454
DraI	TTTAAA	2 cut(s)	1458, 1746
Eam1104I	CTCTTC	5 cut(s)	319, 617, 1656, 2037, 2147
EarI	CTCTTC	5 cut(s)	319, 617, 1656, 2037, 2147
Eco130I	CCWWGG	1 cut(s)	1824
Eco47I	GGWCC	4 cut(s)	31, 571, 2399, 2504
Eco57I	CTGAAG	4 cut(s)	222, 956, 1250, 1916
Eco72I	CACGTG	1 cut(s)	2027
Eco88I	CYCGRG	1 cut(s)	1474
EcoT14I	CCWWGG	1 cut(s)	1824
EcoT22I	ATGCAT	2 cut(s)	658, 2332
ErhI	CCWWGG	1 cut(s)	1824
FaeI	CATG	8 cut(s)	614, 660, 706, 757, 1505, 1858, 2257, 2428
FaqI	GGGAC	4 cut(s)	301, 584, 1552, 2511
FatI	CATG	8 cut(s)	610, 656, 702, 753, 1501, 1854, 2253, 2424
FauI	CCCGC	1 cut(s)	1021
FauNDI	CATATG	3 cut(s)	810, 1492, 2332
FbaI	TGATCA	3 cut(s)	277, 588, 2427
FblI	GTMKAC	2 cut(s)	1685, 2390
Fnu4HI	GCNGC	6 cut(s)	237, 1026, 1101, 1186, 2020, 2223
FokI	GGATG	8 cut(s)	502, 1816, 1871, 1895, 2207, 2339, 2463, 2707
Fsp4HI	GCNGC	6 cut(s)	237, 1026, 1101, 1186, 2020, 2223
FspBI	CTAG	7 cut(s)	135, 162, 189, 995, 1481, 1847, 2394
FspI	TGCGCA	2 cut(s)	2023, 2651
GlaI	GCGC	3 cut(s)	1189, 2023, 2651
GluI	GCNGC	6 cut(s)	237, 1026, 1101, 1186, 2020, 2223
GsuI	CTGGAG	2 cut(s)	1782, 1928
HaeIII	GGCC	1 cut(s)	1185
HapII	CCGG	5 cut(s)	256, 405, 1818, 1880, 2402
HhaI	GCGC	3 cut(s)	1190, 2024, 2652
Hin1II	CATG	8 cut(s)	614, 660, 706, 757, 1505, 1858, 2257, 2428
Hin6I	GCGC	3 cut(s)	1188, 2022, 2650
HinP1I	GCGC	3 cut(s)	1188, 2022, 2650
HincII	GTYRAC	5 cut(s)	385, 977, 1613, 2155, 2391
HindII	GTYRAC	5 cut(s)	385, 977, 1613, 2155, 2391
HindIII	AAGCTT	2 cut(s)	1736, 2307
HpaI	GTTAAC	2 cut(s)	385, 2155
HpaII	CCGG	5 cut(s)	256, 405, 1818, 1880, 2402
HphI	GGTGA	3 cut(s)	287, 1727, 2593
Hpy166II	GTNNAC	9 cut(s)	364, 385, 977, 1613, 1686, 1888, 2155, 2391, 2399
Hpy8I	GTNNAC	9 cut(s)	364, 385, 977, 1613, 1686, 1888, 2155, 2391, 2399
HpyAV	CCTTC	7 cut(s)	87, 549, 1169, 1387, 1940, 2578, 2592
HpyCH4III	ACNGT	3 cut(s)	646, 1708, 2098
HpyCH4IV	ACGT	2 cut(s)	1682, 2026
HpyF10VI	GCNNNNNNNGC	3 cut(s)	137, 236, 797
HpyF3I	CTNAG	4 cut(s)	81, 542, 1555, 2050
HpySE526I	ACGT	2 cut(s)	1682, 2026
Hsp92II	CATG	8 cut(s)	614, 660, 706, 757, 1505, 1858, 2257, 2428
HspAI	GCGC	3 cut(s)	1188, 2022, 2650
Kpn2I	TCCGGA	1 cut(s)	1879
Ksp22I	TGATCA	3 cut(s)	277, 588, 2427
KspAI	GTTAAC	2 cut(s)	385, 2155
Kzo9I	GATC	9 cut(s)	277, 337, 583, 588, 1820, 1977, 2404, 2427, 2454
LguI	GCTCTTC	2 cut(s)	2037, 2147
LmnI	GCTCC	4 cut(s)	244, 418, 595, 991
Lsp1109I	GCAGC	5 cut(s)	248, 1012, 1087, 2006, 2209
LweI	GCATC	6 cut(s)	948, 1380, 1612, 1957, 2317, 2376
MaeI	CTAG	7 cut(s)	135, 162, 189, 995, 1481, 1847, 2394
MaeII	ACGT	2 cut(s)	1682, 2026
MaeIII	GTNAC	9 cut(s)	112, 458, 515, 1157, 1925, 2098, 2298, 2620, 2638
MalI	GATC	9 cut(s)	279, 339, 585, 590, 1822, 1979, 2406, 2429, 2456
MboI	GATC	9 cut(s)	277, 337, 583, 588, 1820, 1977, 2404, 2427, 2454
MfeI	CAATTG	1 cut(s)	2002
MflI	RGATCY	3 cut(s)	583, 1820, 1977
MhlI	GDGCHC	1 cut(s)	2032
MluI	ACGCGT	1 cut(s)	869
MlyI	GAGTC	5 cut(s)	35, 192, 1486, 1547, 1574
MmeI	TCCRAC	3 cut(s)	690, 1752, 2304
Mph1103I	ATGCAT	2 cut(s)	658, 2332
MroI	TCCGGA	1 cut(s)	1879
MroXI	GAANNNNTTC	1 cut(s)	2070
MslI	CAYNNNNRTG	1 cut(s)	426
MspI	CCGG	5 cut(s)	256, 405, 1818, 1880, 2402
MspR9I	CCNGG	1 cut(s)	405
MssI	GTTTAAAC	1 cut(s)	1458
MunI	CAATTG	1 cut(s)	2002
Mva1269I	GAATGC	2 cut(s)	220, 472
MvnI	CGCG	3 cut(s)	871, 1188, 1899
MwoI	GCNNNNNNNGC	3 cut(s)	137, 236, 797
NciI	CCSGG	1 cut(s)	405
NdeI	CATATG	3 cut(s)	810, 1492, 2332
NdeII	GATC	9 cut(s)	277, 337, 583, 588, 1820, 1977, 2404, 2427, 2454
NheI	GCTAGC	1 cut(s)	994
NlaIII	CATG	8 cut(s)	614, 660, 706, 757, 1505, 1858, 2257, 2428
NlaIV	GGNNCC	9 cut(s)	33, 572, 1143, 1184, 1349, 1416, 1822, 1979, 2056
NmeAIII	GCCGAG	1 cut(s)	604
NmuCI	GTSAC	5 cut(s)	458, 1925, 2098, 2298, 2638
NsbI	TGCGCA	2 cut(s)	2023, 2651
NsiI	ATGCAT	2 cut(s)	658, 2332
NspI	RCATGY	2 cut(s)	660, 2257
NspV	TTCGAA	1 cut(s)	1966
PaeR7I	CTCGAG	1 cut(s)	1474
PagI	TCATGA	2 cut(s)	610, 2424
PciI	ACATGT	1 cut(s)	2253
PciSI	GCTCTTC	2 cut(s)	2037, 2147
PcsI	WCGNNNNNNNCGW	1 cut(s)	857
PctI	GAATGC	2 cut(s)	220, 472
PdmI	GAANNNNTTC	1 cut(s)	2070
PflFI	GACNNNGTC	1 cut(s)	2502
PflMI	CCANNNNNTGG	1 cut(s)	1648
PkrI	GCNGC	6 cut(s)	238, 1027, 1102, 1187, 2021, 2224
PleI	GAGTC	5 cut(s)	35, 191, 1485, 1547, 1573
PmaCI	CACGTG	1 cut(s)	2027
PmeI	GTTTAAAC	1 cut(s)	1458
PmlI	CACGTG	1 cut(s)	2027
PpsI	GAGTC	5 cut(s)	35, 191, 1485, 1547, 1573
Ppu21I	YACGTR	1 cut(s)	2027
PscI	ACATGT	1 cut(s)	2253
PshBI	ATTAAT	1 cut(s)	222
PsiI	TTATAA	3 cut(s)	1245, 2012, 2313
PspCI	CACGTG	1 cut(s)	2027
PspN4I	GGNNCC	9 cut(s)	33, 572, 1143, 1184, 1349, 1416, 1822, 1979, 2056
PspPI	GGNCC	5 cut(s)	31, 571, 1183, 2399, 2504
PstI	CTGCAG	1 cut(s)	1383
PstNI	CAGNNNCTG	2 cut(s)	242, 1951
PsuI	RGATCY	3 cut(s)	583, 1820, 1977
PsyI	GACNNNGTC	1 cut(s)	2502
RsaI	GTAC	5 cut(s)	199, 661, 1889, 1955, 2000
RsaNI	GTAC	5 cut(s)	198, 660, 1888, 1954, 1999
RseI	CAYNNNNRTG	1 cut(s)	426
SalI	GTCGAC	1 cut(s)	2389
SapI	GCTCTTC	2 cut(s)	2037, 2147
SatI	GCNGC	6 cut(s)	237, 1026, 1101, 1186, 2020, 2223
Sau3AI	GATC	9 cut(s)	277, 337, 583, 588, 1820, 1977, 2404, 2427, 2454
Sau96I	GGNCC	5 cut(s)	31, 571, 1183, 2399, 2504
SchI	GAGTC	5 cut(s)	35, 192, 1486, 1547, 1574
ScrFI	CCNGG	1 cut(s)	405
SduI	GDGCHC	1 cut(s)	2032
SfaNI	GCATC	6 cut(s)	948, 1380, 1612, 1957, 2317, 2376
SfcI	CTRYAG	2 cut(s)	1379, 1957
Sfr274I	CTCGAG	1 cut(s)	1474
SfuI	TTCGAA	1 cut(s)	1966
SinI	GGWCC	4 cut(s)	31, 571, 2399, 2504
SlaI	CTCGAG	1 cut(s)	1474
SmiI	ATTTAAAT	1 cut(s)	1746
SmiMI	CAYNNNNRTG	1 cut(s)	426
SmlI	CTYRAG	5 cut(s)	341, 347, 446, 1474, 2669
SmoI	CTYRAG	5 cut(s)	341, 347, 446, 1474, 2669
SpeI	ACTAGT	1 cut(s)	2393
SspI	AATATT	3 cut(s)	435, 1132, 1355
SspMI	CTAG	7 cut(s)	135, 162, 189, 995, 1481, 1847, 2394
StyD4I	CCNGG	1 cut(s)	403
StyI	CCWWGG	1 cut(s)	1824
SwaI	ATTTAAAT	1 cut(s)	1746
TaaI	ACNGT	3 cut(s)	646, 1708, 2098
TaiI	ACGT	2 cut(s)	1685, 2029
TaqII	GACCGA	1 cut(s)	588
TatI	WGTACW	4 cut(s)	659, 1887, 1953, 1998
TauI	GCSGC	1 cut(s)	1188
TscAI	CASTG	1 cut(s)	463
TseFI	GTSAC	5 cut(s)	458, 1925, 2098, 2298, 2638
TseI	GCWGC	5 cut(s)	236, 1025, 1100, 2019, 2222
Tsp45I	GTSAC	5 cut(s)	458, 1925, 2098, 2298, 2638
TspGWI	ACGGA	2 cut(s)	1442, 2199
TspRI	CASTG	1 cut(s)	463
Tth111I	GACNNNGTC	1 cut(s)	2502
Van91I	CCANNNNNTGG	1 cut(s)	1648
VpaK11BI	GGWCC	4 cut(s)	31, 571, 2399, 2504
VspI	ATTAAT	1 cut(s)	222
XapI	RAATTY	8 cut(s)	177, 352, 476, 794, 818, 1747, 2357, 2663
XceI	RCATGY	2 cut(s)	660, 2257
XhoI	CTCGAG	1 cut(s)	1474
XmiI	GTMKAC	2 cut(s)	1685, 2390
XmnI	GAANNNNTTC	1 cut(s)	2070
XspI	CTAG	7 cut(s)	135, 162, 189, 995, 1481, 1847, 2394
Zsp2I	ATGCAT	2 cut(s)	658, 2332

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.