Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013189

Physical Location & Seq

Genomic Coordinates Forward (+)

10989469 .. 10992174

Gene Structure 2706 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1148600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2706 bp

ATGATGACCTCACTGAGATTTTCAATCTCTTTGGACACGTTTGATTCTTCAAACAAGTTCAATTCTTCTTTTTGCTACGGTCATGCATATGTTAGCAGAGCTTGTGTAGTTAATGCTCTGAATTGGGGAAATCGGATTAAGGTTTCTGGGTTCCCATTCGAATTGTCGGGCACTTCGGAACTGAGTCAGATGGGTGAGGAAACCAATTTGAGCTTGGTTGAGGGAAACCCGGATTTTCGACACGAAAGTGAGAAGGGCTCTGGGGGGCCAAAGAAAGAGAGTAGGAGAGAAAAGGATTCGAAGTTAAGTTCAAGAAAGAGTAGGTGGGTGAGGGAATTGGAGAATTTGTTTGTGAATGGTGGTGAATTTGATGTTGATTATTCTGTTATTAGCTCTCACTTGAGCATGGAGCATTGTAACGATATTTTGAGACGGCTAGAGAGGTGTAATGATGTCAAAGCTCTTAGATTCTTCGAGTGGATGAGAAGCAACGGGAAATTAGAGCGCAATGTGAGCGCTTTTAATTCAGTTTTAAGAGTGATGGGTAGGAGAGAAGATTGGGATGCAGCTGAAAAATTGGTTCAGGAATTGCTTGCTGGTTTGGGAGGTGAGCTGAATTATCAGGTTTTCAACACCCTTATTTATGCGTGTTGTAAGTTGGGGCGCGCTGAGTTGGGAGCCAAGTGGTTTCGAATGATGCTGGATCACAGGATCCAGCCGAACATTGCAACTTTTGGTATGCTAATGGGACTTTACCAGAAGGGTTGGAATGTTGAGGAGGCGGAGTTTACTTTCTCTCAAATGAGGAACTGTGGAATTGTATGTCAATCAGCGTACTCTGCGATGATCACAATTTACACCCGCTTGAACTTGTATGACAAAGCGGAAGAGGTCATTGGCTTGATGAGAGAAGATAGAGTGAGATTGAATTTGGATAATTGGTTGGTAATGATTAATGCCTATTGTCAGCAGGGTAAAGTAGACGATGCTGAAGTAGTACTGGTCTCCATGCAAGAAGCGGGGTTTTCTCCAAACATTATTGCATACAATACTTTGATAACTGGATATGGGAAGGCGTCTAAAATGGATGCTGCTCATCACCTATTTCTGGGCATAAAGAACGCTGGATTAGAGCCTGATGAAACAACTTACCGTTCTATGATTGAAGGTTGGGGTCGAGCTGACAAGTATAAGGAAGCAGAATGGTACTACAAAGAGCTCAAGCGGTTGGGGTACAAGCCTAATTCGTCTAACCTGTACACACTAGTAAACTTGCAAGCCAAGCATGAGGATGAAGAGGGGGCCATCAGGACTCTTGACGATATGCTGACAATGGGGTGCCAATATTCCTCGATTCTTGGTACTCTTCTGCAAGCATACGAGAAGGTAGGAAGGGTAGATAAAGTGCCTCGCCTTTTGAGAGGTTCTTTCTATCAACATATTCTTGTCAGCCAGACTTCTTGTTCCATTCTTGTCATGGCTTATGTGAAACACTGCTTGGTGGATGATACTATGAAAGTGTTGAGGGAGAAACTGTGGAAGGACCCGCCTTTTGAAGATAACTTGTATCATTTGTTAATTTGCTCGTGTAAAGAGTTGGGTCGTCTTGAAGATGCTGTTAAAATATACAAGCAAATGCTGAGACATTTTAACAAGCCAAACATGCACATCATGTGCACAATGATTGACATCTATAGCATCATGGGCCTATTCACAGAAGCGGAAAAAACTTACGTGGAGTTAAAATCTTCTGGAATCGTATTGGACCTGATTGCCTATAGCATTGCTGTGAGAATGTATGTAAAAGCTGGTTCTCTGGAAGATGCTTGCTCTGTTCTAGAGGCAATGGAAGAACAGGAGGGAATTGTTCCAGACATTTACATGTTCCGTGATATGCTCCGGATTTATCAACGATGTGGCAGGCTTGATAAGTTGAAAGACCTGTACTATAAACTCTTGAAGAGTGGAGTGACTTGGGATCGGGAAATGTACAACTGTGTCATAAACTGCTGTTCTCATGCTTTGCCAGTCGATGAGATCTCAGAGATCTTTGATGAGATGCTTCAATGTGGTTTTGTTCCTAATACCATAACCTTCAATGTCATGCTCGATGTATACGGGAAAGCAAGGCTTCTGAAGAAAGCTAGGGAGTTGTTCAGGATGGCCCAAAAGTGGGGTTTGGTTGACATGATCTCTTACAATACTATTATAGCTGCTTACGGGCGAAATAAAGATTTCAGAAGCATGTCTTCAACATTTCAAGAGATGCAGTTCAAGGGCTTTTCAGTTTCCCTGGAAGCCTACAATTCCATGTTGGATGCTTATGGGAAGGAAAGCCAAATGGAAAGATTTAGAAGCGTTTTGCAGAGAATGAAGAAAACAAGCTGTGCTTCTGACCATTACACGTACAACATTATGATCAATGTCTATGGAGAGCAAGGATGGATTGATGAAGTTGCTGGTGTGCTGACTGAGTTGAAAGAATGCGAACTTGGACCTGATCTGTGCAGCTATAACACGTTGATTAAGGCATACGGAATTGCAGGAATGGTCGAAGATGCTGTCCACTTGGTCAAGGAAATGAGAGAAAATGGTATAGAGCCTGATAAGATAACCTATGTCAATCTTATCGCTGCGCTGCAAAGAAACGATGAATATTTGGAGGCTGTAAAATGGTCCCTGTGGATGAAGCAGATGGGGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000079 GO:0000428 GO:0001932 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005675 GO:0006139 GO:0006259 GO:0006281 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016591 GO:0019219 GO:0019220 GO:0019222 GO:0030880 GO:0031323 GO:0031326 GO:0031399 GO:0031974 GO:0031981 GO:0032268 GO:0032806 GO:0032991 GO:0033554 GO:0034641 GO:0042325 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043549 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045859 GO:0046483 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051174 GO:0051246 GO:0051252 GO:0051338 GO:0051716 GO:0051726 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0071704 GO:0071900 GO:0080090 GO:0090304 GO:0090575 GO:1901360 GO:1902494 GO:1902554 GO:1902911 GO:1903506 GO:1904029 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

902

Amino Acids

103.54

Weight (kDa)

6.01

Isoelectric Point (pI)

36.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	209 - 249	1.8e-06	PPR repeat family
PPR_3	PF13812	307 - 358	4.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	344 - 390	2.7e-12	PPR repeat family
PPR_3	PF13812	654 - 708	7.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	663 - 706	2.8e-08	PPR repeat family
PPR_long	PF17177	741 - 877	3.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	753 - 812	2.4e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	767 - 810	1.8e-10	PPR repeat family
PPR_3	PF13812	822 - 879	1.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	833 - 880	6.8e-13	PPR repeat family
PPR_1	PF12854	833 - 862	3.8e-07	PPR repeat
PPR	PF01535	837 - 866	6.5e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013189)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30825
fragaria_vesca	FvH4_1g00580
malus_domestica	MD15G1148600.v1.1
prunus_persica	Prupe.7G266300_v2.0.a1
pyrus_communis	pycom15g13290 pycom15g13300
rosa_chinensis	RchiOBHm_Chr2g0085211
rosa_laevigata	RLG00000015667
rosa_multiflora	Rmu_sc0008510.1_g000001 Rmu_sc0008647.1_g000001 Rmu_sc0011989.1_g000006
rosa_roxburghii	Rroxscaffold_2G00155560
rosa_rugosa	Rorug01G0458400
rosa_samantha	Rh2AG007000 Rh2BG007600 Rh2CG008400 Rh2DG008700
rosa_wichuraiana	Rw2G000650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1338
AccI	GTMKAC	2 cut(s)	981, 2115
AccII	CGCG	1 cut(s)	666
AccIII	TCCGGA	1 cut(s)	1899
AciI	CCGC	7 cut(s)	782, 862, 884, 1019, 1225, 1547, 1721
AclWI	GGATC	4 cut(s)	706, 711, 719, 1986
AcsI	RAATTY	3 cut(s)	343, 365, 928
AcuI	CTGAAG	2 cut(s)	1011, 2156
AcyI	GRCGYC	1 cut(s)	1076
AfaI	GTAC	9 cut(s)	836, 999, 1208, 1235, 1259, 1363, 1946, 1991, 2408
AfeI	AGCGCT	1 cut(s)	517
AfiI	CCNNNNNNNGG	3 cut(s)	1108, 2172, 2173
AflIII	ACRYGT	4 cut(s)	36, 1881, 2403, 2517
AhlI	ACTAGT	1 cut(s)	1264
AjnI	CCWGG	1 cut(s)	2292
AjuI	GAANNNNNNNTTGG	6 cut(s)	879, 911, 1481, 1513, 2475, 2507
AleI	CACNNNNGTG	1 cut(s)	246
Alw21I	GWGCWC	2 cut(s)	1221, 1679
Alw26I	GTCTC	3 cut(s)	424, 1009, 1636
Alw44I	GTGCAC	1 cut(s)	1675
AlwI	GGATC	4 cut(s)	706, 711, 719, 1986
Aor13HI	TCCGGA	1 cut(s)	1899
Aor51HI	AGCGCT	1 cut(s)	517
AoxI	GGCC	4 cut(s)	266, 1302, 1705, 2163
ApaLI	GTGCAC	1 cut(s)	1675
ApeKI	GCWGC	6 cut(s)	566, 1091, 2213, 2508, 2633, 2638
ApoI	RAATTY	3 cut(s)	343, 365, 928
ArsI	GACNNNNNNTTYG	4 cut(s)	1535, 1567, 2547, 2579
AseI	ATTAAT	1 cut(s)	954
AspLEI	GCGC	5 cut(s)	507, 518, 666, 668, 2638
AspS9I	GGNCC	8 cut(s)	266, 1302, 1543, 1705, 1765, 2164, 2495, 2676
AsuC2I	CCSGG	1 cut(s)	230
AsuHPI	GGTGA	5 cut(s)	206, 340, 374, 620, 1091
AsuII	TTCGAA	3 cut(s)	159, 299, 691
AvaII	GGWCC	4 cut(s)	1543, 1765, 2495, 2676
BaeGI	GKGCMC	2 cut(s)	173, 1679
BamHI	GGATCC	1 cut(s)	711
BanI	GGYRCC	1 cut(s)	1338
BanII	GRGCYC	2 cut(s)	260, 1221
BauI	CACGAG	1 cut(s)	1585
BbsI	GAAGAC	1 cut(s)	2241
Bbv12I	GWGCWC	2 cut(s)	1221, 1679
BbvI	GCAGC	6 cut(s)	578, 1078, 2200, 2520, 2620, 2625
BccI	CCATC	6 cut(s)	184, 535, 1313, 2155, 2436, 2689
BceAI	ACGGC	1 cut(s)	449
BciT130I	CCWGG	1 cut(s)	2294
BclI	TGATCA	2 cut(s)	846, 2418
BcnI	CCSGG	1 cut(s)	230
BcoDI	GTCTC	3 cut(s)	424, 1009, 1636
BcuI	ACTAGT	1 cut(s)	1264
BfaI	CTAG	4 cut(s)	437, 1265, 1838, 2145
BfmI	CTRYAG	2 cut(s)	1693, 1777
BfoI	RGCGCY	1 cut(s)	519
BglII	AGATCT	2 cut(s)	2037, 2046
BisI	GCNGC	6 cut(s)	567, 1092, 2214, 2509, 2634, 2639
BlsI	GCNGC	6 cut(s)	568, 1093, 2215, 2510, 2635, 2640
BmcAI	AGTACT	1 cut(s)	999
Bme1390I	CCNGG	2 cut(s)	230, 2294
Bme18I	GGWCC	4 cut(s)	1543, 1765, 2495, 2676
BmgT120I	GGNCC	8 cut(s)	266, 1302, 1543, 1705, 1765, 2164, 2495, 2676
BmiI	GGNNCC	8 cut(s)	152, 267, 679, 713, 1303, 1340, 1545, 2678
BmrFI	CCNGG	2 cut(s)	230, 2294
BpiI	GAAGAC	1 cut(s)	2241
BplI	GAGNNNNNCTC	2 cut(s)	242, 274
Bpu14I	TTCGAA	3 cut(s)	159, 299, 691
BpuEI	CTTGAG	2 cut(s)	421, 1205
BpuMI	CCSGG	1 cut(s)	230
BsaAI	YACGTR	2 cut(s)	1735, 2406
BsaBI	GATNNNNATC	1 cut(s)	1688
BsaHI	GRCGYC	1 cut(s)	1076
BsaI	GGTCTC	1 cut(s)	1009
BsaJI	CCNNGG	1 cut(s)	2292
BsaWI	WCCGGW	1 cut(s)	1899
BsaXI	ACNNNNNCTCC	6 cut(s)	332, 362, 770, 800, 1520, 1550
Bsc4I	CCNNNNNNNGG	3 cut(s)	1108, 2172, 2173
Bse1I	ACTGG	3 cut(s)	1005, 1066, 2027
Bse3DI	GCAATG	4 cut(s)	514, 723, 1782, 1851
Bse8I	GATNNNNATC	1 cut(s)	1688
BseAI	TCCGGA	1 cut(s)	1899
BseBI	CCWGG	1 cut(s)	2294
BseDI	CCNNGG	1 cut(s)	2292
BseGI	GGATG	9 cut(s)	486, 568, 1093, 1297, 1510, 2166, 2323, 2447, 2691
BseJI	GATNNNNATC	1 cut(s)	1688
BseLI	CCNNNNNNNGG	3 cut(s)	1108, 2172, 2173
BseMI	GCAATG	4 cut(s)	514, 723, 1782, 1851
BseMII	CTCAG	6 cut(s)	5, 173, 660, 1631, 2055, 2463
BseNI	ACTGG	3 cut(s)	1005, 1066, 2027
BsePI	GCGCGC	1 cut(s)	664
BseRI	GAGGAG	1 cut(s)	791
BseSI	GKGCMC	2 cut(s)	173, 1679
BseXI	GCAGC	6 cut(s)	578, 1078, 2200, 2520, 2620, 2625
BsgI	GTGCAG	1 cut(s)	2527
Bsh1236I	CGCG	1 cut(s)	666
BshFI	GGCC	4 cut(s)	268, 1304, 1707, 2165
BshNI	GGYRCC	1 cut(s)	1338
BsiHKAI	GWGCWC	2 cut(s)	1221, 1679
BsiSI	CCGG	2 cut(s)	230, 1900
BslFI	GGGAC	2 cut(s)	762, 2662
BslI	CCNNNNNNNGG	3 cut(s)	1108, 2172, 2173
BsmAI	GTCTC	3 cut(s)	424, 1009, 1636
BsmBI	CGTCTC	1 cut(s)	424
BsmFI	GGGAC	2 cut(s)	762, 2662
BsmI	GAATGC	1 cut(s)	2489
BsnI	GGCC	4 cut(s)	268, 1304, 1707, 2165
Bso31I	GGTCTC	1 cut(s)	1009
Bsp119I	TTCGAA	3 cut(s)	159, 299, 691
Bsp1286I	GDGCHC	4 cut(s)	173, 260, 1221, 1679
Bsp13I	TCCGGA	1 cut(s)	1899
Bsp1407I	TGTACA	2 cut(s)	1257, 1989
Bsp143I	GATC	9 cut(s)	703, 711, 846, 1978, 2037, 2046, 2190, 2418, 2500
BspACI	CCGC	7 cut(s)	782, 862, 884, 1019, 1225, 1547, 1721
BspANI	GGCC	4 cut(s)	268, 1304, 1707, 2165
BspCNI	CTCAG	6 cut(s)	6, 174, 661, 1632, 2054, 2464
BspEI	TCCGGA	1 cut(s)	1899
BspFNI	CGCG	1 cut(s)	666
BspLI	GGNNCC	8 cut(s)	152, 267, 679, 713, 1303, 1340, 1545, 2678
BspPI	GGATC	4 cut(s)	706, 711, 719, 1986
BspT104I	TTCGAA	3 cut(s)	159, 299, 691
BspT107I	GGYRCC	1 cut(s)	1338
BspTNI	GGTCTC	1 cut(s)	1009
BsrDI	GCAATG	4 cut(s)	514, 723, 1782, 1851
BsrGI	TGTACA	2 cut(s)	1257, 1989
BsrI	ACTGG	3 cut(s)	1005, 1066, 2027
BssECI	CCNNGG	1 cut(s)	2292
BssHII	GCGCGC	1 cut(s)	664
BssMI	GATC	9 cut(s)	703, 711, 846, 1978, 2037, 2046, 2190, 2418, 2500
BssNAI	GTATAC	1 cut(s)	2116
BssNI	GRCGYC	1 cut(s)	1076
BssSI	CACGAG	1 cut(s)	1585
Bst1107I	GTATAC	1 cut(s)	2116
Bst2BI	CACGAG	1 cut(s)	1585
Bst2UI	CCWGG	1 cut(s)	2294
Bst4CI	ACNGT	5 cut(s)	80, 812, 1154, 1536, 1997
Bst6I	CTCTTC	4 cut(s)	882, 1290, 1371, 1955
BstACI	GRCGYC	1 cut(s)	1076
BstAUI	TGTACA	2 cut(s)	1257, 1989
BstBAI	YACGTR	2 cut(s)	1735, 2406
BstBI	TTCGAA	3 cut(s)	159, 299, 691
BstC8I	GCNNGC	6 cut(s)	594, 666, 1278, 1374, 1828, 1922
BstDEI	CTNAG	7 cut(s)	14, 182, 464, 669, 1640, 2041, 2472
BstF5I	GGATG	9 cut(s)	486, 568, 1093, 1297, 1510, 2166, 2323, 2447, 2691
BstFNI	CGCG	1 cut(s)	666
BstH2I	RGCGCY	1 cut(s)	519
BstHHI	GCGC	5 cut(s)	507, 518, 666, 668, 2638
BstKTI	GATC	9 cut(s)	706, 714, 849, 1981, 2040, 2049, 2193, 2421, 2503
BstMAI	GTCTC	3 cut(s)	424, 1009, 1636
BstMBI	GATC	9 cut(s)	703, 711, 846, 1978, 2037, 2046, 2190, 2418, 2500
BstMWI	GCNNNNNNNGC	5 cut(s)	513, 839, 1282, 1663, 1704
BstNI	CCWGG	1 cut(s)	2294
BstNSI	RCATGY	3 cut(s)	1666, 1885, 2248
BstSCI	CCNGG	2 cut(s)	228, 2292
BstSFI	CTRYAG	2 cut(s)	1693, 1777
BstSLI	GKGCMC	2 cut(s)	173, 1679
BstUI	CGCG	1 cut(s)	666
BstV1I	GCAGC	6 cut(s)	578, 1078, 2200, 2520, 2620, 2625
BstV2I	GAAGAC	1 cut(s)	2241
BstX2I	RGATCY	3 cut(s)	711, 2037, 2046
BstYI	RGATCY	3 cut(s)	711, 2037, 2046
BstZ17I	GTATAC	1 cut(s)	2116
BsuRI	GGCC	4 cut(s)	268, 1304, 1707, 2165
BtgZI	GCGATG	1 cut(s)	857
BtsCI	GGATG	9 cut(s)	486, 568, 1093, 1297, 1510, 2166, 2323, 2447, 2691
BtsI	GCAGTG	1 cut(s)	1492
BtsIMutI	CAGTG	2 cut(s)	11, 1492
Cac8I	GCNNGC	6 cut(s)	594, 666, 1278, 1374, 1828, 1922
CfoI	GCGC	5 cut(s)	507, 518, 666, 668, 2638
Cfr13I	GGNCC	8 cut(s)	266, 1302, 1543, 1705, 1765, 2164, 2495, 2676
CseI	GACGC	1 cut(s)	1065
Csp6I	GTAC	9 cut(s)	835, 998, 1207, 1234, 1258, 1362, 1945, 1990, 2407
CviQI	GTAC	9 cut(s)	835, 998, 1207, 1234, 1258, 1362, 1945, 1990, 2407
DdeI	CTNAG	7 cut(s)	14, 182, 464, 669, 1640, 2041, 2472
DpnI	GATC	9 cut(s)	705, 713, 848, 1980, 2039, 2048, 2192, 2420, 2502
DpnII	GATC	9 cut(s)	703, 711, 846, 1978, 2037, 2046, 2190, 2418, 2500
Eam1104I	CTCTTC	4 cut(s)	882, 1290, 1371, 1955
EarI	CTCTTC	4 cut(s)	882, 1290, 1371, 1955
EciI	GGCGGA	1 cut(s)	797
Ecl136II	GAGCTC	1 cut(s)	1219
Eco24I	GRGCYC	2 cut(s)	260, 1221
Eco31I	GGTCTC	1 cut(s)	1009
Eco47I	GGWCC	4 cut(s)	1543, 1765, 2495, 2676
Eco47III	AGCGCT	1 cut(s)	517
Eco53kI	GAGCTC	1 cut(s)	1219
Eco57I	CTGAAG	2 cut(s)	1011, 2156
EcoICRI	GAGCTC	1 cut(s)	1219
EcoO109I	RGGNCCY	1 cut(s)	1543
EcoRII	CCWGG	1 cut(s)	2292
EcoT22I	ATGCAT	1 cut(s)	88
EcoT38I	GRGCYC	2 cut(s)	260, 1221
Esp3I	CGTCTC	1 cut(s)	424
FalI	AAGNNNNNCTT	6 cut(s)	2115, 2147, 2233, 2265, 2374, 2406
FaqI	GGGAC	2 cut(s)	762, 2662
FauI	CCCGC	3 cut(s)	869, 1012, 1554
FauNDI	CATATG	1 cut(s)	88
FbaI	TGATCA	2 cut(s)	846, 2418
FblI	GTMKAC	2 cut(s)	981, 2115
Fnu4HI	GCNGC	6 cut(s)	567, 1092, 2214, 2509, 2634, 2639
FokI	GGATG	9 cut(s)	493, 575, 1100, 1304, 1517, 2173, 2330, 2454, 2698
FriOI	GRGCYC	2 cut(s)	260, 1221
Fsp4HI	GCNGC	6 cut(s)	567, 1092, 2214, 2509, 2634, 2639
FspBI	CTAG	4 cut(s)	437, 1265, 1838, 2145
GlaI	GCGC	5 cut(s)	506, 517, 665, 667, 2637
GluI	GCNGC	6 cut(s)	567, 1092, 2214, 2509, 2634, 2639
HaeII	RGCGCY	1 cut(s)	519
HaeIII	GGCC	4 cut(s)	268, 1304, 1707, 2165
HapII	CCGG	2 cut(s)	230, 1900
HgaI	GACGC	1 cut(s)	1065
HhaI	GCGC	5 cut(s)	507, 518, 666, 668, 2638
Hin1I	GRCGYC	1 cut(s)	1076
Hin6I	GCGC	5 cut(s)	505, 516, 664, 666, 2636
HinP1I	GCGC	5 cut(s)	505, 516, 664, 666, 2636
HincII	GTYRAC	1 cut(s)	2185
HindII	GTYRAC	1 cut(s)	2185
HinfI	GANTC	7 cut(s)	44, 184, 296, 468, 1312, 1354, 1755
HpaII	CCGG	2 cut(s)	230, 1900
HphI	GGTGA	5 cut(s)	206, 340, 374, 620, 1091
Hpy166II	GTNNAC	8 cut(s)	789, 982, 1260, 1270, 1677, 2116, 2185, 2566
Hpy188I	TCNGA	8 cut(s)	120, 135, 178, 189, 2044, 2136, 2240, 2395
Hpy8I	GTNNAC	8 cut(s)	789, 982, 1260, 1270, 1677, 2116, 2185, 2566
HpyAV	CCTTC	9 cut(s)	247, 754, 1066, 1160, 1378, 1386, 1534, 2104, 2323
HpyCH4III	ACNGT	5 cut(s)	80, 812, 1154, 1536, 1997
HpyCH4IV	ACGT	4 cut(s)	38, 1734, 2405, 2519
HpyF10VI	GCNNNNNNNGC	5 cut(s)	513, 839, 1282, 1663, 1704
HpyF3I	CTNAG	7 cut(s)	14, 182, 464, 669, 1640, 2041, 2472
HpySE526I	ACGT	4 cut(s)	38, 1734, 2405, 2519
Hsp92I	GRCGYC	1 cut(s)	1076
HspAI	GCGC	5 cut(s)	505, 516, 664, 666, 2636
Kpn2I	TCCGGA	1 cut(s)	1899
Ksp22I	TGATCA	2 cut(s)	846, 2418
Kzo9I	GATC	9 cut(s)	703, 711, 846, 1978, 2037, 2046, 2190, 2418, 2500
LmnI	GCTCC	3 cut(s)	409, 677, 1902
Lsp1109I	GCAGC	6 cut(s)	578, 1078, 2200, 2520, 2620, 2625
MaeI	CTAG	4 cut(s)	437, 1265, 1838, 2145
MaeII	ACGT	4 cut(s)	38, 1734, 2405, 2519
MaeIII	GTNAC	2 cut(s)	416, 1969
MalI	GATC	9 cut(s)	705, 713, 848, 1980, 2039, 2048, 2192, 2420, 2502
MboI	GATC	9 cut(s)	703, 711, 846, 1978, 2037, 2046, 2190, 2418, 2500
MflI	RGATCY	3 cut(s)	711, 2037, 2046
MhlI	GDGCHC	4 cut(s)	173, 260, 1221, 1679
MlyI	GAGTC	2 cut(s)	193, 1306
MmeI	TCCRAC	2 cut(s)	746, 2295
Mph1103I	ATGCAT	1 cut(s)	88
MroI	TCCGGA	1 cut(s)	1899
MslI	CAYNNNNRTG	5 cut(s)	87, 246, 1290, 1787, 1880
MspA1I	CMGCKG	1 cut(s)	569
MspI	CCGG	2 cut(s)	230, 1900
MspR9I	CCNGG	2 cut(s)	230, 2294
Mva1269I	GAATGC	1 cut(s)	2489
MvaI	CCWGG	1 cut(s)	2294
MvnI	CGCG	1 cut(s)	666
MwoI	GCNNNNNNNGC	5 cut(s)	513, 839, 1282, 1663, 1704
NciI	CCSGG	1 cut(s)	230
NdeI	CATATG	1 cut(s)	88
NdeII	GATC	9 cut(s)	703, 711, 846, 1978, 2037, 2046, 2190, 2418, 2500
NlaIV	GGNNCC	8 cut(s)	152, 267, 679, 713, 1303, 1340, 1545, 2678
NmuCI	GTSAC	1 cut(s)	1969
NsiI	ATGCAT	1 cut(s)	88
NspI	RCATGY	3 cut(s)	1666, 1885, 2248
NspV	TTCGAA	3 cut(s)	159, 299, 691
OliI	CACNNNNGTG	1 cut(s)	246
PauI	GCGCGC	1 cut(s)	664
PciI	ACATGT	1 cut(s)	1881
PctI	GAATGC	1 cut(s)	2489
PfeI	GAWTC	5 cut(s)	44, 296, 468, 1354, 1755
PkrI	GCNGC	6 cut(s)	568, 1093, 2215, 2510, 2635, 2640
PleI	GAGTC	2 cut(s)	192, 1306
PpsI	GAGTC	2 cut(s)	192, 1306
Ppu21I	YACGTR	2 cut(s)	1735, 2406
PpuMI	RGGWCCY	1 cut(s)	1543
PscI	ACATGT	1 cut(s)	1881
PshBI	ATTAAT	1 cut(s)	954
Psp124BI	GAGCTC	1 cut(s)	1221
Psp5II	RGGWCCY	1 cut(s)	1543
Psp6I	CCWGG	1 cut(s)	2292
PspGI	CCWGG	1 cut(s)	2292
PspN4I	GGNNCC	8 cut(s)	152, 267, 679, 713, 1303, 1340, 1545, 2678
PspPI	GGNCC	8 cut(s)	266, 1302, 1543, 1705, 1765, 2164, 2495, 2676
PspPPI	RGGWCCY	1 cut(s)	1543
PsuI	RGATCY	3 cut(s)	711, 2037, 2046
PteI	GCGCGC	1 cut(s)	664
PvuII	CAGCTG	1 cut(s)	569
RsaI	GTAC	9 cut(s)	836, 999, 1208, 1235, 1259, 1363, 1946, 1991, 2408
RsaNI	GTAC	9 cut(s)	835, 998, 1207, 1234, 1258, 1362, 1945, 1990, 2407
RseI	CAYNNNNRTG	5 cut(s)	87, 246, 1290, 1787, 1880
SacI	GAGCTC	1 cut(s)	1221
SatI	GCNGC	6 cut(s)	567, 1092, 2214, 2509, 2634, 2639
Sau3AI	GATC	9 cut(s)	703, 711, 846, 1978, 2037, 2046, 2190, 2418, 2500
Sau96I	GGNCC	8 cut(s)	266, 1302, 1543, 1705, 1765, 2164, 2495, 2676
ScaI	AGTACT	1 cut(s)	999
SchI	GAGTC	2 cut(s)	193, 1306
ScrFI	CCNGG	2 cut(s)	230, 2294
SduI	GDGCHC	4 cut(s)	173, 260, 1221, 1679
SfcI	CTRYAG	2 cut(s)	1693, 1777
SfuI	TTCGAA	3 cut(s)	159, 299, 691
SinI	GGWCC	4 cut(s)	1543, 1765, 2495, 2676
SmiMI	CAYNNNNRTG	5 cut(s)	87, 246, 1290, 1787, 1880
SmlI	CTYRAG	2 cut(s)	400, 1220
SmoI	CTYRAG	2 cut(s)	400, 1220
SpeI	ACTAGT	1 cut(s)	1264
SsiI	CCGC	7 cut(s)	782, 862, 884, 1019, 1225, 1547, 1721
SspI	AATATT	2 cut(s)	1346, 2657
SspMI	CTAG	4 cut(s)	437, 1265, 1838, 2145
SstI	GAGCTC	1 cut(s)	1221
StyD4I	CCNGG	2 cut(s)	228, 2292
TaaI	ACNGT	5 cut(s)	80, 812, 1154, 1536, 1997
TaiI	ACGT	4 cut(s)	41, 1737, 2408, 2522
TatI	WGTACW	4 cut(s)	997, 1257, 1944, 1989
TfiI	GAWTC	5 cut(s)	44, 296, 468, 1354, 1755
TscAI	CASTG	2 cut(s)	18, 1499
TseFI	GTSAC	1 cut(s)	1969
TseI	GCWGC	6 cut(s)	566, 1091, 2213, 2508, 2633, 2638
Tsp45I	GTSAC	1 cut(s)	1969
TspDTI	ATGAA	7 cut(s)	1155, 1308, 1529, 2387, 2466, 2667, 2702
TspGWI	ACGGA	2 cut(s)	1877, 2550
TspRI	CASTG	2 cut(s)	18, 1499
VneI	GTGCAC	1 cut(s)	1675
VpaK11BI	GGWCC	4 cut(s)	1543, 1765, 2495, 2676
VspI	ATTAAT	1 cut(s)	954
XapI	RAATTY	3 cut(s)	343, 365, 928
XbaI	TCTAGA	1 cut(s)	1837
XceI	RCATGY	3 cut(s)	1666, 1885, 2248
XcmI	CCANNNNNNNNNTGG	2 cut(s)	211, 1474
XmiI	GTMKAC	2 cut(s)	981, 2115
XspI	CTAG	4 cut(s)	437, 1265, 1838, 2145
ZrmI	AGTACT	1 cut(s)	999
Zsp2I	ATGCAT	1 cut(s)	88

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.