AT4G31420

zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013264
Physical Location & Seq
Reverse (-)
15245813 .. 15248058
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31420.2

Sequence Viewer

Length: 1215 bp
ATGCCTGGTTTAACATGTAACGCGTGTAACATGGAGTTCAAGGACGAAGAAGAACGTAACCTTCATTACAAATCCGATTGGCACCGCTACAATCTCAAGCGCAAGGTGGCTGGAGTTCCTGGAGTTACAGAAGCCTTGTTTGAGGCTAGACAATCTGCTCTTGCTCAGGAGAAGAATAAATCAAATGAAGCACCTATGCTTTATACTTGTGCAATCTGTGCTAAAGGTTACAGGAGTTCCAAGGCTCATGAGCAGCATCTTCAGTCGCGTAGCCATGTTTTGCGGGTTTCACAAGGGACATCAATCAACGGAGAGGAGGATATAGCGATTATCAGGCAACTTCCTCGCCGTGTTCAACACAGGGGTTCCATTGATGATGATAGTGAAGATGAATGGGTGGAGGTTGATTCTGACGAGGAATTGGCTGCTGAAGAAGCCTCTGATTCTTTGTCTAAGTTGAATGTGAATGAGTCTGGATCTGCTGAAGATATGGATGATGATGGTGATGCAGATAAGTATGAGCTGGATCCAACCTGCTGTTTGATGTGTGACAAAAAACATAAGACCTTAGAGAGCTGTATGCTTCACATGCATAAGCATCATGGTTTCTTTATTCCCGATATTGAGTATCTCAAGGATCCTGAAGGGCTTCTCACTTACCTTGGTCTTAAGGTCAAGAGAGACTTCATGTGTCTGTACTGCAATGAGCTGTGCCGTCCATTCAGCAGCTTAGAAGCTGTAAGAAAACATATGGAAGCAAAGAGTCATTGCAAACTGCATTATGGTGATGGTGATGATGAAGAAGATGCTGAGCTAGAAGAGTTTTATGACTACAGCAGCAGCTATGTTGATGAAGCTGGAAAGCAAATTGTTGTATCTGGTGAAACAGACAATACTGTAGAACTCGTTGGTGGTTCTGAGCTCTTGATCACGGAGAAGTCCGAGAACACAACAACGTCTAAAACTCTCGGGTCCCGTGAATTCATGCGTTACTACAGACAGAAGCCACGCCCAACTTCTCAAGACAGCAACCAGATAATTGCCTCTTTATCTTCAAGGTACAAGAGCCTGGGTCTGAAGACAGTACCGTCGAAAGAGGAGACTTTGAGGATGAAAGTGCGCAAGGAGATGAGCAAGAGAGGCGAGACAATGCGTACCAAGATCGGAGTGAAGAGTAATGTCATAAGAAACTTGCCCAATAACGTCCCATATTAG

Protein Analysis

404

Amino Acids

46.0

Weight (kDa)

5.44

Isoelectric Point (pI)

50.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_2 PF12756 180 - 281 6.9e-28 C2H2 type zinc-finger (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24500 AT4G31420 AT4G31420
fragaria_vesca FvH4_2g36910
malus_domestica MD08G1131000.v1.1 MD15G1108800.v1.1
prunus_persica Prupe.1G461000_v2.0.a1
pyrus_communis pycom15g09940
rosa_chinensis RchiOBHm_Chr6g0304871
rosa_laevigata RLG00000010943
rosa_multiflora Rmu_ssc0000278.1_g000006
rosa_roxburghii Rroxscaffold_7G00163390
rosa_rugosa Rorug06G0336000
rosa_samantha Rh6AG447100 Rh6BG454400 Rh6CG460600 Rh6DG447600
rosa_wichuraiana Rw6G038920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1087
Acc16I TGCGCA 1 cut(s) 1121
Acc36I ACCTGC 1 cut(s) 542
AccB1I GGYRCC 1 cut(s) 81
AccII CGCG 2 cut(s) 23, 268
AciI CCGC 2 cut(s) 85, 283
AclWI GGATC 5 cut(s) 484, 521, 534, 632, 645
AcsI RAATTY 1 cut(s) 980
AcuI CTGAAG 5 cut(s) 245, 450, 504, 663, 1097
AfaI GTAC 4 cut(s) 698, 1061, 1086, 1156
AflII CTTAAG 1 cut(s) 668
AflIII ACRYGT 2 cut(s) 14, 21
AgsI TTSAA 4 cut(s) 40, 356, 460, 1056
AjnI CCWGG 3 cut(s) 4, 118, 1068
AluBI AGCT 9 cut(s) 523, 576, 709, 729, 737, 814, 843, 857, 922
AluI AGCT 9 cut(s) 523, 576, 709, 729, 737, 814, 843, 857, 922
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 3 cut(s) 675, 1094, 1139
AlwI GGATC 5 cut(s) 484, 521, 534, 632, 645
Ama87I CYCGRG 1 cut(s) 968
ApeKI GCWGC 5 cut(s) 253, 425, 726, 837, 840
ApoI RAATTY 1 cut(s) 980
Asp700I GAANNNNTTC 1 cut(s) 648
AspLEI GCGC 2 cut(s) 102, 1122
AspS9I GGNCC 1 cut(s) 972
AsuHPI GGTGA 4 cut(s) 515, 797, 803, 893
AvaI CYCGRG 1 cut(s) 968
AvaII GGWCC 1 cut(s) 972
BamHI GGATCC 2 cut(s) 526, 637
BanI GGYRCC 1 cut(s) 81
BanII GRGCYC 1 cut(s) 924
BbsI GAAGAC 1 cut(s) 1085
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 5 cut(s) 265, 412, 738, 849, 852
BccI CCATC 2 cut(s) 494, 782
BceAI ACGGC 2 cut(s) 333, 699
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 3 cut(s) 6, 120, 1070
BclI TGATCA 1 cut(s) 927
BcoDI GTCTC 3 cut(s) 675, 1094, 1139
BfaI CTAG 2 cut(s) 147, 815
BfmI CTRYAG 3 cut(s) 832, 897, 994
BfrI CTTAAG 1 cut(s) 668
BfuAI ACCTGC 1 cut(s) 542
BisI GCNGC 5 cut(s) 254, 426, 727, 838, 841
BlpI GCTNAGC 1 cut(s) 810
BlsI GCNGC 5 cut(s) 255, 427, 728, 839, 842
Bme1390I CCNGG 3 cut(s) 6, 120, 1070
Bme18I GGWCC 1 cut(s) 972
BmeT110I CYCGRG 1 cut(s) 968
BmgT120I GGNCC 1 cut(s) 972
BmiI GGNNCC 6 cut(s) 83, 367, 528, 639, 973, 974
BmrFI CCNGG 3 cut(s) 6, 120, 1070
BmsI GCATC 4 cut(s) 265, 496, 607, 796
BpiI GAAGAC 1 cut(s) 1085
BpmI CTGGAG 2 cut(s) 132, 141
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 810
BpuEI CTTGAG 3 cut(s) 80, 617, 1005
BsaJI CCNNGG 3 cut(s) 240, 661, 1069
Bse3DI GCAATG 2 cut(s) 709, 766
BseBI CCWGG 3 cut(s) 6, 120, 1070
BseDI CCNNGG 3 cut(s) 240, 661, 1069
BseGI GGATG 2 cut(s) 499, 1116
BseMI GCAATG 2 cut(s) 709, 766
BseMII CTCAG 3 cut(s) 179, 801, 909
BseRI GAGGAG 2 cut(s) 329, 1112
BseXI GCAGC 5 cut(s) 265, 412, 738, 849, 852
Bsh1236I CGCG 2 cut(s) 23, 268
BshNI GGYRCC 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 924
BsiHKCI CYCGRG 1 cut(s) 968
BslFI GGGAC 3 cut(s) 310, 958, 1190
BsmAI GTCTC 3 cut(s) 675, 1094, 1139
BsmFI GGGAC 3 cut(s) 310, 958, 1190
BsoBI CYCGRG 1 cut(s) 968
Bsp1286I GDGCHC 1 cut(s) 924
Bsp143I GATC 5 cut(s) 476, 526, 637, 927, 1161
Bsp1720I GCTNAGC 1 cut(s) 810
BspACI CCGC 2 cut(s) 85, 283
BspCNI CTCAG 3 cut(s) 178, 802, 910
BspFNI CGCG 2 cut(s) 23, 268
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 6 cut(s) 83, 367, 528, 639, 973, 974
BspMI ACCTGC 1 cut(s) 542
BspPI GGATC 5 cut(s) 484, 521, 534, 632, 645
BspT107I GGYRCC 1 cut(s) 81
BspTI CTTAAG 1 cut(s) 668
BsrDI GCAATG 2 cut(s) 709, 766
BssECI CCNNGG 3 cut(s) 240, 661, 1069
BssMI GATC 5 cut(s) 476, 526, 637, 927, 1161
BssT1I CCWWGG 2 cut(s) 240, 661
Bst2UI CCWGG 3 cut(s) 6, 120, 1070
Bst4CI ACNGT 3 cut(s) 898, 1084, 1089
Bst6I CTCTTC 2 cut(s) 813, 1166
BstAFI CTTAAG 1 cut(s) 668
BstAPI GCANNNNNTGC 1 cut(s) 218
BstDEI CTNAG 6 cut(s) 165, 453, 568, 730, 810, 918
BstF5I GGATG 2 cut(s) 499, 1116
BstFNI CGCG 2 cut(s) 23, 268
BstHHI GCGC 2 cut(s) 102, 1122
BstKTI GATC 5 cut(s) 479, 529, 640, 930, 1164
BstMAI GTCTC 3 cut(s) 675, 1094, 1139
BstMBI GATC 5 cut(s) 476, 526, 637, 927, 1161
BstMWI GCNNNNNNNGC 4 cut(s) 218, 434, 589, 1140
BstNI CCWGG 3 cut(s) 6, 120, 1070
BstNSI RCATGY 2 cut(s) 18, 592
BstSCI CCNGG 3 cut(s) 4, 118, 1068
BstSFI CTRYAG 3 cut(s) 832, 897, 994
BstUI CGCG 2 cut(s) 23, 268
BstV1I GCAGC 5 cut(s) 265, 412, 738, 849, 852
BstV2I GAAGAC 1 cut(s) 1085
BstX2I RGATCY 3 cut(s) 476, 526, 637
BstYI RGATCY 3 cut(s) 476, 526, 637
BtsCI GGATG 2 cut(s) 499, 1116
BveI ACCTGC 1 cut(s) 542
CciI TCATGA 1 cut(s) 247
CfoI GCGC 2 cut(s) 102, 1122
Cfr13I GGNCC 1 cut(s) 972
Csp6I GTAC 4 cut(s) 697, 1060, 1085, 1155
CviAII CATG 8 cut(s) 15, 31, 248, 275, 589, 602, 688, 985
CviQI GTAC 4 cut(s) 697, 1060, 1085, 1155
DdeI CTNAG 6 cut(s) 165, 453, 568, 730, 810, 918
DpnI GATC 5 cut(s) 478, 528, 639, 929, 1163
DpnII GATC 5 cut(s) 476, 526, 637, 927, 1161
DrdI GACNNNNNNGTC 1 cut(s) 1087
DseDI GACNNNNNNGTC 1 cut(s) 1087
Eam1104I CTCTTC 2 cut(s) 813, 1166
EarI CTCTTC 2 cut(s) 813, 1166
Ecl136II GAGCTC 1 cut(s) 922
Eco130I CCWWGG 2 cut(s) 240, 661
Eco24I GRGCYC 1 cut(s) 924
Eco47I GGWCC 1 cut(s) 972
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 5 cut(s) 245, 450, 504, 663, 1097
Eco88I CYCGRG 1 cut(s) 968
EcoICRI GAGCTC 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 972
EcoRI GAATTC 1 cut(s) 980
EcoRII CCWGG 3 cut(s) 4, 118, 1068
EcoT14I CCWWGG 2 cut(s) 240, 661
EcoT22I ATGCAT 1 cut(s) 594
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 2 cut(s) 240, 661
FaeI CATG 8 cut(s) 18, 34, 251, 278, 592, 605, 691, 988
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 3 cut(s) 310, 958, 1190
FatI CATG 8 cut(s) 14, 30, 247, 274, 588, 601, 687, 984
FauI CCCGC 1 cut(s) 276
FauNDI CATATG 1 cut(s) 750
FbaI TGATCA 1 cut(s) 927
Fnu4HI GCNGC 5 cut(s) 254, 426, 727, 838, 841
FokI GGATG 2 cut(s) 506, 1123
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 5 cut(s) 254, 426, 727, 838, 841
FspBI CTAG 2 cut(s) 147, 815
FspI TGCGCA 1 cut(s) 1121
GlaI GCGC 2 cut(s) 101, 1121
GluI GCNGC 5 cut(s) 254, 426, 727, 838, 841
GsuI CTGGAG 2 cut(s) 132, 141
HhaI GCGC 2 cut(s) 102, 1122
Hin1II CATG 8 cut(s) 18, 34, 251, 278, 592, 605, 691, 988
Hin6I GCGC 2 cut(s) 100, 1120
HinP1I GCGC 2 cut(s) 100, 1120
HinfI GANTC 4 cut(s) 407, 443, 470, 763
HphI GGTGA 4 cut(s) 515, 797, 803, 893
Hpy188I TCNGA 7 cut(s) 76, 412, 442, 919, 943, 1077, 1166
Hpy188III TCNNGA 8 cut(s) 167, 248, 474, 617, 641, 676, 925, 1022
Hpy99I CGWCG 1 cut(s) 1093
HpyAV CCTTC 2 cut(s) 71, 638
HpyCH4III ACNGT 3 cut(s) 898, 1084, 1089
HpyCH4IV ACGT 3 cut(s) 55, 956, 1203
HpyCH4V TGCA 6 cut(s) 212, 509, 592, 702, 771, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 434, 589, 1140
HpyF3I CTNAG 6 cut(s) 165, 453, 568, 730, 810, 918
HpySE526I ACGT 3 cut(s) 55, 956, 1203
Hsp92II CATG 8 cut(s) 18, 34, 251, 278, 592, 605, 691, 988
HspAI GCGC 2 cut(s) 100, 1120
KflI GGGWCCC 1 cut(s) 972
Ksp22I TGATCA 1 cut(s) 927
Kzo9I GATC 5 cut(s) 476, 526, 637, 927, 1161
Lsp1109I GCAGC 5 cut(s) 265, 412, 738, 849, 852
LweI GCATC 4 cut(s) 265, 496, 607, 796
MaeI CTAG 2 cut(s) 147, 815
MaeII ACGT 3 cut(s) 55, 956, 1203
MaeIII GTNAC 7 cut(s) 17, 26, 56, 124, 227, 548, 989
MalI GATC 5 cut(s) 478, 528, 639, 929, 1163
MboI GATC 5 cut(s) 476, 526, 637, 927, 1161
MflI RGATCY 3 cut(s) 476, 526, 637
MhlI GDGCHC 1 cut(s) 924
MluCI AATT 4 cut(s) 419, 867, 980, 1038
MluI ACGCGT 1 cut(s) 21
MlyI GAGTC 2 cut(s) 479, 772
MmeI TCCRAC 1 cut(s) 554
Mph1103I ATGCAT 1 cut(s) 594
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 2 cut(s) 11, 669
MslI CAYNNNNRTG 1 cut(s) 783
MspCI CTTAAG 1 cut(s) 668
MspR9I CCNGG 3 cut(s) 6, 120, 1070
MvaI CCWGG 3 cut(s) 6, 120, 1070
MvnI CGCG 2 cut(s) 23, 268
MwoI GCNNNNNNNGC 4 cut(s) 218, 434, 589, 1140
NdeI CATATG 1 cut(s) 750
NdeII GATC 5 cut(s) 476, 526, 637, 927, 1161
NlaIII CATG 8 cut(s) 18, 34, 251, 278, 592, 605, 691, 988
NlaIV GGNNCC 6 cut(s) 83, 367, 528, 639, 973, 974
NmuCI GTSAC 1 cut(s) 548
NsbI TGCGCA 1 cut(s) 1121
NsiI ATGCAT 1 cut(s) 594
NspI RCATGY 2 cut(s) 18, 592
PagI TCATGA 1 cut(s) 247
PciI ACATGT 1 cut(s) 14
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 2 cut(s) 407, 443
PfoI TCCNGGA 1 cut(s) 118
PkrI GCNGC 5 cut(s) 255, 427, 728, 839, 842
PleI GAGTC 2 cut(s) 478, 771
PpsI GAGTC 2 cut(s) 478, 771
PpuMI RGGWCCY 1 cut(s) 972
PscI ACATGT 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 924
Psp5II RGGWCCY 1 cut(s) 972
Psp6I CCWGG 3 cut(s) 4, 118, 1068
PspGI CCWGG 3 cut(s) 4, 118, 1068
PspN4I GGNNCC 6 cut(s) 83, 367, 528, 639, 973, 974
PspPI GGNCC 1 cut(s) 972
PspPPI RGGWCCY 1 cut(s) 972
PsuI RGATCY 3 cut(s) 476, 526, 637
RsaI GTAC 4 cut(s) 698, 1061, 1086, 1156
RsaNI GTAC 4 cut(s) 697, 1060, 1085, 1155
RseI CAYNNNNRTG 1 cut(s) 783
SacI GAGCTC 1 cut(s) 924
SaqAI TTAA 2 cut(s) 11, 669
SatI GCNGC 5 cut(s) 254, 426, 727, 838, 841
Sau3AI GATC 5 cut(s) 476, 526, 637, 927, 1161
Sau96I GGNCC 1 cut(s) 972
SchI GAGTC 2 cut(s) 479, 772
ScrFI CCNGG 3 cut(s) 6, 120, 1070
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 4 cut(s) 265, 496, 607, 796
SfcI CTRYAG 3 cut(s) 832, 897, 994
SinI GGWCC 1 cut(s) 972
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 4 cut(s) 95, 632, 668, 1020
SmoI CTYRAG 4 cut(s) 95, 632, 668, 1020
Sse9I AATT 4 cut(s) 419, 867, 980, 1038
SsiI CCGC 2 cut(s) 85, 283
SspMI CTAG 2 cut(s) 147, 815
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 3 cut(s) 4, 118, 1068
StyI CCWWGG 2 cut(s) 240, 661
TaaI ACNGT 3 cut(s) 898, 1084, 1089
TaiI ACGT 3 cut(s) 58, 959, 1206
TaqI TCGA 1 cut(s) 1091
TasI AATT 4 cut(s) 419, 867, 980, 1038
TatI WGTACW 1 cut(s) 696
TfiI GAWTC 2 cut(s) 407, 443
Tru1I TTAA 2 cut(s) 11, 669
Tru9I TTAA 2 cut(s) 11, 669
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 5 cut(s) 253, 425, 726, 837, 840
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 8 cut(s) 53, 201, 405, 676, 813, 867, 973, 1127
TspGWI ACGGA 2 cut(s) 324, 947
Vha464I CTTAAG 1 cut(s) 668
VpaK11BI GGWCC 1 cut(s) 972
XapI RAATTY 1 cut(s) 980
XceI RCATGY 2 cut(s) 18, 592
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 2 cut(s) 147, 815
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.