MD08G1131000.v1.1

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013264
Physical Location & Seq
Forward (+)
12379430 .. 12381882
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1131000.v1.1.491

Sequence Viewer

Length: 858 bp
ATGTCAGGGCTAACATGCAACTCTTGCAACAAGGAGTTTCTGGACGACTCGGAGCAGAAGCTCCATTACAAGTCCGAATGGCACCGCTACAATCTCAAGCGCAAGGTAGCTGGGGTTCCTGGAGTGACAGAAGCATTATTTATAGCGAGACAAGCTGCACTTGCTCAGGAGAAAAACGGTTTGAGTGAAAGCCCGATGCTCTACAGTTGTGGTCTTTGTGGCAAAGGGTATAGAAGTTCTAAGGCTCATGCTGAGCACCTCAAGTCACGAAGTCACATCCTGCGGGCCTCTCAAGGGGCCAGTGAACAAGAAGAGAAGCCAATAATTAGGCCACTTCCGAGTCGTGTTGTTAACAAAGCTCCCCCGAAAAGAGAGGCAAATGATGAGGAAACTGATGAAAGTGAAGATGAGTGGGTGGAGTTTGATCCTGACGAAGACTTGTCAAAATCATTGACTGATATGAATGTGGATGAGCATGCATCCGAAGAGGATATGGATCAAGATGACGACTTTGAGGAGTTGGATCCAACTTGTTGCTTTATGTGCGATCATGATCACGACACCATAGAGAGCTGCATGGTTCACATGCACAAACAACATGGATTCTTCATTCCTGATATCGAGTATTTAAAGGATCCAAAAGGCCTCCTTACTTATCTTGGTCTCAAGGTTAAAAGGGATTTCATGTGTCTGTACTGCAATGATAGGCGCCATCCTTTTAATAGTTTGGAAGCAGTTAGGAAGCATATGGTGGCAAAAAGCCACTGCAAGGTACATTACGGTGATGGTGACGATGAAGAGGAAGCTGAGTTGGAAGAGTTCTATGATTACAGCAGCAGGCTGGCAAACAACTCGTAG

Protein Analysis

286

Amino Acids

32.75

Weight (kDa)

4.97

Isoelectric Point (pI)

64.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_2 PF12756 179 - 279 9.1e-28 C2H2 type zinc-finger (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24500 AT4G31420 AT4G31420
fragaria_vesca FvH4_2g36910
malus_domestica MD08G1131000.v1.1 MD15G1108800.v1.1
prunus_persica Prupe.1G461000_v2.0.a1
pyrus_communis pycom15g09940
rosa_chinensis RchiOBHm_Chr6g0304871
rosa_laevigata RLG00000010943
rosa_multiflora Rmu_ssc0000278.1_g000006
rosa_roxburghii Rroxscaffold_7G00163390
rosa_rugosa Rorug06G0336000
rosa_samantha Rh6AG447100 Rh6BG454400 Rh6CG460600 Rh6DG447600
rosa_wichuraiana Rw6G038920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 81, 708
AciI CCGC 2 cut(s) 85, 283
AclWI GGATC 6 cut(s) 419, 504, 518, 531, 629, 642
AcyI GRCGYC 1 cut(s) 709
AfaI GTAC 2 cut(s) 695, 774
AfiI CCNNNNNNNGG 2 cut(s) 294, 769
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 6 cut(s) 61, 110, 155, 359, 573, 806
AluI AGCT 6 cut(s) 61, 110, 155, 359, 573, 806
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 142, 668
AlwI GGATC 6 cut(s) 419, 504, 518, 531, 629, 642
AoxI GGCC 4 cut(s) 285, 297, 329, 643
ApeKI GCWGC 3 cut(s) 155, 573, 834
AspLEI GCGC 2 cut(s) 102, 711
AspS9I GGNCC 2 cut(s) 285, 297
AsuHPI GGTGA 2 cut(s) 794, 800
BamHI GGATCC 2 cut(s) 523, 634
BanI GGYRCC 2 cut(s) 81, 708
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 3 cut(s) 142, 560, 846
BccI CCATC 2 cut(s) 720, 779
BcgI CGANNNNNNTGC 1 cut(s) 834
BciT130I CCWGG 1 cut(s) 120
BclI TGATCA 1 cut(s) 553
BcoDI GTCTC 2 cut(s) 142, 668
BfmI CTRYAG 1 cut(s) 202
BfoI RGCGCY 1 cut(s) 712
BisI GCNGC 3 cut(s) 156, 574, 835
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 3 cut(s) 157, 575, 836
Bme1390I CCNGG 1 cut(s) 120
BmgT120I GGNCC 2 cut(s) 285, 297
BmiI GGNNCC 6 cut(s) 83, 117, 298, 525, 636, 710
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 186, 488
BpiI GAAGAC 1 cut(s) 441
BpmI CTGGAG 1 cut(s) 141
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 252
BpuEI CTTGAG 4 cut(s) 80, 245, 276, 650
BsaBI GATNNNNATC 2 cut(s) 495, 552
BsaHI GRCGYC 1 cut(s) 709
BsaI GGTCTC 1 cut(s) 668
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 769
Bse1I ACTGG 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 706
Bse8I GATNNNNATC 2 cut(s) 495, 552
BseBI CCWGG 1 cut(s) 120
BseGI GGATG 4 cut(s) 276, 475, 479, 712
BseJI GATNNNNATC 2 cut(s) 495, 552
BseLI CCNNNNNNNGG 2 cut(s) 294, 769
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 3 cut(s) 179, 243, 798
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 3 cut(s) 142, 560, 846
BseYI CCCAGC 1 cut(s) 110
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 4 cut(s) 287, 299, 331, 645
BshNI GGYRCC 2 cut(s) 81, 708
BsiHKAI GWGCWC 1 cut(s) 258
BslI CCNNNNNNNGG 2 cut(s) 294, 769
BsmAI GTCTC 2 cut(s) 142, 668
BsnI GGCC 4 cut(s) 287, 299, 331, 645
Bso31I GGTCTC 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 6 cut(s) 424, 496, 523, 547, 553, 634
Bsp1720I GCTNAGC 1 cut(s) 252
BspACI CCGC 2 cut(s) 85, 283
BspANI GGCC 4 cut(s) 287, 299, 331, 645
BspCNI CTCAG 3 cut(s) 178, 244, 799
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 6 cut(s) 83, 117, 298, 525, 636, 710
BspPI GGATC 6 cut(s) 419, 504, 518, 531, 629, 642
BspT107I GGYRCC 2 cut(s) 81, 708
BspTNI GGTCTC 1 cut(s) 668
BsrDI GCAATG 1 cut(s) 706
BsrI ACTGG 1 cut(s) 300
BssMI GATC 6 cut(s) 424, 496, 523, 547, 553, 634
BssNI GRCGYC 1 cut(s) 709
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 179, 206, 782
Bst6I CTCTTC 4 cut(s) 306, 480, 792, 810
BstACI GRCGYC 1 cut(s) 709
BstAPI GCANNNNNTGC 1 cut(s) 24
BstC8I GCNNGC 4 cut(s) 285, 477, 839, 843
BstDEI CTNAG 4 cut(s) 165, 240, 252, 807
BstF5I GGATG 4 cut(s) 276, 475, 479, 712
BstH2I RGCGCY 1 cut(s) 712
BstHHI GCGC 2 cut(s) 102, 711
BstKTI GATC 6 cut(s) 427, 499, 526, 550, 556, 637
BstMAI GTCTC 2 cut(s) 142, 668
BstMBI GATC 6 cut(s) 424, 496, 523, 547, 553, 634
BstMWI GCNNNNNNNGC 4 cut(s) 24, 152, 161, 543
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 3 cut(s) 18, 479, 589
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 202
BstV1I GCAGC 3 cut(s) 142, 560, 846
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 2 cut(s) 523, 634
BstYI RGATCY 2 cut(s) 523, 634
BsuRI GGCC 4 cut(s) 287, 299, 331, 645
BtsCI GGATG 4 cut(s) 276, 475, 479, 712
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 2 cut(s) 307, 763
Cac8I GCNNGC 4 cut(s) 285, 477, 839, 843
CciI TCATGA 1 cut(s) 550
CfoI GCGC 2 cut(s) 102, 711
Cfr13I GGNCC 2 cut(s) 285, 297
Csp6I GTAC 2 cut(s) 694, 773
CviAII CATG 8 cut(s) 15, 248, 476, 551, 577, 586, 599, 685
CviQI GTAC 2 cut(s) 694, 773
DdeI CTNAG 4 cut(s) 165, 240, 252, 807
DinI GGCGCC 1 cut(s) 710
DpnI GATC 6 cut(s) 426, 498, 525, 549, 555, 636
DpnII GATC 6 cut(s) 424, 496, 523, 547, 553, 634
DraI TTTAAA 1 cut(s) 630
Eam1104I CTCTTC 4 cut(s) 306, 480, 792, 810
EarI CTCTTC 4 cut(s) 306, 480, 792, 810
Eco147I AGGCCT 1 cut(s) 645
Eco31I GGTCTC 1 cut(s) 668
Eco32I GATATC 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 118
EcoRV GATATC 1 cut(s) 619
EcoT22I ATGCAT 1 cut(s) 481
EgeI GGCGCC 1 cut(s) 710
EheI GGCGCC 1 cut(s) 710
FaeI CATG 8 cut(s) 18, 251, 479, 554, 580, 589, 602, 688
FalI AAGNNNNNCTT 4 cut(s) 144, 176, 633, 665
FatI CATG 8 cut(s) 14, 247, 475, 550, 576, 585, 598, 684
FauI CCCGC 1 cut(s) 276
FauNDI CATATG 1 cut(s) 747
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 3 cut(s) 156, 574, 835
FokI GGATG 4 cut(s) 263, 466, 482, 699
Fsp4HI GCNGC 3 cut(s) 156, 574, 835
GlaI GCGC 2 cut(s) 101, 710
GluI GCNGC 3 cut(s) 156, 574, 835
GsaI CCCAGC 1 cut(s) 114
GsuI CTGGAG 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 712
HaeIII GGCC 4 cut(s) 287, 299, 331, 645
HhaI GCGC 2 cut(s) 102, 711
Hin1I GRCGYC 1 cut(s) 709
Hin1II CATG 8 cut(s) 18, 251, 479, 554, 580, 589, 602, 688
Hin6I GCGC 2 cut(s) 100, 709
HinP1I GCGC 2 cut(s) 100, 709
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 3 cut(s) 47, 340, 603
HpaI GTTAAC 1 cut(s) 352
HphI GGTGA 2 cut(s) 794, 800
Hpy166II GTNNAC 3 cut(s) 305, 352, 583
Hpy188I TCNGA 4 cut(s) 52, 76, 339, 484
Hpy188III TCNNGA 8 cut(s) 41, 167, 267, 428, 500, 551, 557, 614
Hpy8I GTNNAC 3 cut(s) 305, 352, 583
HpyCH4III ACNGT 3 cut(s) 179, 206, 782
HpyCH4V TGCA 8 cut(s) 18, 27, 158, 479, 576, 589, 699, 768
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 152, 161, 543
HpyF3I CTNAG 4 cut(s) 165, 240, 252, 807
Hsp92I GRCGYC 1 cut(s) 709
Hsp92II CATG 8 cut(s) 18, 251, 479, 554, 580, 589, 602, 688
HspAI GCGC 2 cut(s) 100, 709
KasI GGCGCC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 553
KspAI GTTAAC 1 cut(s) 352
Kzo9I GATC 6 cut(s) 424, 496, 523, 547, 553, 634
LmnI GCTCC 3 cut(s) 52, 66, 364
Lsp1109I GCAGC 3 cut(s) 142, 560, 846
LweI GCATC 2 cut(s) 186, 488
MaeIII GTNAC 4 cut(s) 124, 264, 272, 788
MalI GATC 6 cut(s) 426, 498, 525, 549, 555, 636
MboI GATC 6 cut(s) 424, 496, 523, 547, 553, 634
MboII GAAGA 7 cut(s) 323, 416, 446, 497, 598, 809, 827
MflI RGATCY 2 cut(s) 523, 634
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 1 cut(s) 324
Mly113I GGCGCC 1 cut(s) 709
MlyI GAGTC 2 cut(s) 41, 349
MmeI TCCRAC 3 cut(s) 501, 551, 792
MnlI CCTC 8 cut(s) 269, 298, 367, 379, 481, 508, 656, 793
Mph1103I ATGCAT 1 cut(s) 481
MseI TTAA 4 cut(s) 351, 629, 672, 720
MslI CAYNNNNRTG 1 cut(s) 780
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 4 cut(s) 24, 152, 161, 543
NarI GGCGCC 1 cut(s) 709
NdeI CATATG 1 cut(s) 747
NdeII GATC 6 cut(s) 424, 496, 523, 547, 553, 634
NlaIII CATG 8 cut(s) 18, 251, 479, 554, 580, 589, 602, 688
NlaIV GGNNCC 6 cut(s) 83, 117, 298, 525, 636, 710
NmuCI GTSAC 4 cut(s) 124, 264, 272, 788
NsiI ATGCAT 1 cut(s) 481
NspI RCATGY 3 cut(s) 18, 479, 589
PaeI GCATGC 1 cut(s) 479
PagI TCATGA 1 cut(s) 550
PceI AGGCCT 1 cut(s) 645
PfeI GAWTC 1 cut(s) 603
PfoI TCCNGGA 1 cut(s) 118
PkrI GCNGC 3 cut(s) 157, 575, 836
PleI GAGTC 2 cut(s) 41, 348
PluTI GGCGCC 1 cut(s) 712
PpsI GAGTC 2 cut(s) 41, 348
Psp6I CCWGG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 110
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 6 cut(s) 83, 117, 298, 525, 636, 710
PspPI GGNCC 2 cut(s) 285, 297
PsrI GAACNNNNNNTAC 2 cut(s) 99, 131
PsuI RGATCY 2 cut(s) 523, 634
RsaI GTAC 2 cut(s) 695, 774
RsaNI GTAC 2 cut(s) 694, 773
RseI CAYNNNNRTG 1 cut(s) 780
SaqAI TTAA 4 cut(s) 351, 629, 672, 720
SatI GCNGC 3 cut(s) 156, 574, 835
Sau3AI GATC 6 cut(s) 424, 496, 523, 547, 553, 634
Sau96I GGNCC 2 cut(s) 285, 297
SchI GAGTC 2 cut(s) 41, 349
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 2 cut(s) 186, 488
SfcI CTRYAG 1 cut(s) 202
SfoI GGCGCC 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 780
SmlI CTYRAG 4 cut(s) 95, 260, 291, 665
SmoI CTYRAG 4 cut(s) 95, 260, 291, 665
SphI GCATGC 1 cut(s) 479
Sse9I AATT 1 cut(s) 324
SseBI AGGCCT 1 cut(s) 645
SsiI CCGC 2 cut(s) 85, 283
SspDI GGCGCC 1 cut(s) 708
StuI AGGCCT 1 cut(s) 645
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 179, 206, 782
TaqI TCGA 1 cut(s) 621
TasI AATT 1 cut(s) 324
TatI WGTACW 1 cut(s) 693
TfiI GAWTC 1 cut(s) 603
Tru1I TTAA 4 cut(s) 351, 629, 672, 720
Tru9I TTAA 4 cut(s) 351, 629, 672, 720
TscAI CASTG 2 cut(s) 307, 770
TseFI GTSAC 4 cut(s) 124, 264, 272, 788
TseI GCWGC 3 cut(s) 155, 573, 834
Tsp45I GTSAC 4 cut(s) 124, 264, 272, 788
TspDTI ATGAA 5 cut(s) 411, 476, 598, 673, 810
TspRI CASTG 2 cut(s) 307, 770
XceI RCATGY 3 cut(s) 18, 479, 589
Zsp2I ATGCAT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.