AT4G31810

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014122
Physical Location & Seq
Reverse (-)
15386788 .. 15390430
3643 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31810.1

Sequence Viewer

Length: 1230 bp
ATGCAAACAGTGAAAGCTTTGAGGAGAGTGAGTGAACCCTTACAATGGGTTCGGTCTGTTTCTTATGGAAGACGCTTTTCTGCTCTCCCAAACTATTCCGCATCAGATGCAGATTTCGAAGACCAGGTTCTGGTGGAAGGAAAAGCTAAATCAAGAGCTGCCATTCTCAATAACCCATCTTCTCTCAATGCTCTTTCTGCGCCTATGGTTGGTCGGTTAAAGAGGCTATACGAATCATGGGAAGAGAACCCAGCTATTTCCTTTGTTTTGATGAAGGGTAGCGGTAAAACTTTCTGCTCTGGTGCAGATGTCTTGTCTCTTTATCACTCGATCAATGAAGGAAATACTGAAGAATCTAAACTCTTTTTCGAGAACTTGTACAAGTTTGTATACCTCCAAGGAACGTATTTAAAACCAAATATAGCAATAATGGATGGTGTGACCATGGGTTGTGGTGGTGGAATTTCACTTCCAGGGATGTTTCGTGTGGCTACAGATAAAACTGTGTTGGCCCATCCAGAGGTCCAAATTGGTTTTCATCCTGATGCAGGAGCTTCCTATTATCTTTCACGGCTTCCTGGTTATTTAGGGGAATACTTGGCTCTAACGGGGCAGAAACTTAATGGTGTCGAAATGATAGCATGTGGCCTTGCCACCCACTATTGCTTAAACGCGAGACTTCCGTTGATTGAAGAGAGGATTGGTAAACTGTTGACCGATGATCCTGCTGTCATTGAGGATTCTCTTGCTCAATATGGTGATCTTGTTTACCCTGACAGTAGCAGCGTACTGCACAAGATAGAGTTGATTGATAAATATTTTGGGCTTGATACCGTTGAAGAAATCATTGAAGCTATGGAAAATGAAGCTGCTAATTCGTGCAATGAATGGTGCAAGAAAACTCTCAAACAGATCAAAGAAGCTTCACCTTTGAGCTTAAAGATTACTTTGCAATCTATACGAGAAGGTAGATTCCAAACCCTTGATCAATGTCTCACACATGAATACCGTATATCCATTTGTGGAGTCTCAAAAGTAGTCTCTGGCGACTTTTGCGAGGGTATTCGAGCCCGTTTGGTAGATAAAGACTTTGCTCCAAAGTGGGATCCTCCACGCCTAGAAGATGTGAGCAAAGACATGGTGGATTGCTACTTCACGCCAGCCTCAGAGCTCGATGATTCAGATTCTGAGTTGAAGCTGCCAACAGCTCAACGAGAGCCTTATTTTTGA

Protein Analysis

409

Amino Acids

45.5

Weight (kDa)

5.1

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 52 - 385 5.8e-116 Enoyl-CoA hydratase/isomerase
ECH_1 PF00378 56 - 221 5.4e-19 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014122)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31810
fragaria_vesca FvH4_2g41110
malus_domestica MD08G1057200.v1.1 MD15G1040200.v1.1
prunus_persica Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1
pyrus_communis pycom08g04670
rosa_chinensis RchiOBHm_Chr6g0299431
rosa_laevigata RLG00000011432
rosa_multiflora Rmu_sc0037301.1_g000004
rosa_roxburghii Rroxscaffold_7G00168690
rosa_rugosa Rorug06G0288100
rosa_samantha Rh6AG400000 Rh6BG408600 Rh6CG414100
rosa_wichuraiana Rw6G035010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 1 cut(s) 390
AccII CGCG 1 cut(s) 674
AciI CCGC 2 cut(s) 99, 282
AclWI GGATC 3 cut(s) 716, 1100, 1113
AcsI RAATTY 1 cut(s) 462
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 2 cut(s) 380, 789
AfiI CCNNNNNNNGG 5 cut(s) 45, 130, 209, 520, 548
AgsI TTSAA 4 cut(s) 692, 839, 851, 1195
AjnI CCWGG 3 cut(s) 123, 472, 577
AjuI GAANNNNNNNTTGG 4 cut(s) 519, 551, 684, 716
Alw21I GWGCWC 1 cut(s) 1173
Alw26I GTCTC 5 cut(s) 321, 670, 998, 1033, 1045
AlwI GGATC 3 cut(s) 716, 1100, 1113
AlwNI CAGNNNCTG 2 cut(s) 130, 1187
AoxI GGCC 2 cut(s) 510, 646
ApeKI GCWGC 4 cut(s) 158, 783, 869, 1198
ApoI RAATTY 1 cut(s) 462
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 2 cut(s) 511, 523
AsuHPI GGTGA 2 cut(s) 770, 918
AsuII TTCGAA 1 cut(s) 117
AvaII GGWCC 1 cut(s) 523
BamHI GGATCC 1 cut(s) 1105
BanII GRGCYC 2 cut(s) 1072, 1173
BbsI GAAGAC 2 cut(s) 76, 126
Bbv12I GWGCWC 1 cut(s) 1173
BbvI GCAGC 4 cut(s) 145, 795, 856, 1185
BccI CCATC 3 cut(s) 184, 428, 522
BceAI ACGGC 1 cut(s) 587
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 3 cut(s) 125, 474, 579
BclI TGATCA 1 cut(s) 985
BcoDI GTCTC 5 cut(s) 321, 670, 998, 1033, 1045
BfaI CTAG 1 cut(s) 1118
BfmI CTRYAG 1 cut(s) 492
BisI GCNGC 4 cut(s) 159, 784, 870, 1199
BlsI GCNGC 4 cut(s) 160, 785, 871, 1200
Bme1390I CCNGG 3 cut(s) 125, 474, 579
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 511, 523
BmiI GGNNCC 1 cut(s) 1107
BmrFI CCNGG 3 cut(s) 125, 474, 579
BmsI GCATC 3 cut(s) 97, 110, 535
BpiI GAAGAC 2 cut(s) 76, 126
Bpu14I TTCGAA 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 397, 444, 473
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 130, 209, 520, 548
Bse3DI GCAATG 1 cut(s) 889
BseBI CCWGG 3 cut(s) 125, 474, 579
BseDI CCNNGG 3 cut(s) 397, 444, 473
BseGI GGATG 4 cut(s) 439, 483, 514, 538
BseLI CCNNNNNNNGG 5 cut(s) 45, 130, 209, 520, 548
BseMI GCAATG 1 cut(s) 889
BseMII CTCAG 2 cut(s) 1179, 1179
BseRI GAGGAG 1 cut(s) 37
BseXI GCAGC 4 cut(s) 145, 795, 856, 1185
BseYI CCCAGC 1 cut(s) 250
BsgI GTGCAG 2 cut(s) 324, 776
Bsh1236I CGCG 1 cut(s) 674
BshFI GGCC 2 cut(s) 512, 648
BsiHKAI GWGCWC 1 cut(s) 1173
BslI CCNNNNNNNGG 5 cut(s) 45, 130, 209, 520, 548
BsmAI GTCTC 5 cut(s) 321, 670, 998, 1033, 1045
BsnI GGCC 2 cut(s) 512, 648
Bsp119I TTCGAA 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 1072, 1173
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 6 cut(s) 330, 721, 760, 912, 985, 1105
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 2 cut(s) 99, 282
BspANI GGCC 2 cut(s) 512, 648
BspCNI CTCAG 2 cut(s) 1178, 1180
BspFNI CGCG 1 cut(s) 674
BspLI GGNNCC 1 cut(s) 1107
BspPI GGATC 3 cut(s) 716, 1100, 1113
BspT104I TTCGAA 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 889
BsrGI TGTACA 1 cut(s) 378
BssECI CCNNGG 3 cut(s) 397, 444, 473
BssMI GATC 6 cut(s) 330, 721, 760, 912, 985, 1105
BssNAI GTATAC 1 cut(s) 391
BssT1I CCWWGG 2 cut(s) 397, 444
Bst1107I GTATAC 1 cut(s) 391
Bst2UI CCWGG 3 cut(s) 125, 474, 579
Bst4CI ACNGT 6 cut(s) 10, 505, 711, 779, 835, 1010
Bst6I CTCTTC 2 cut(s) 237, 687
BstAPI GCANNNNNTGC 1 cut(s) 107
BstAUI TGTACA 1 cut(s) 378
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 1161
BstDEI CTNAG 2 cut(s) 1165, 1188
BstDSI CCRYGG 1 cut(s) 444
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 4 cut(s) 439, 483, 514, 538
BstFNI CGCG 1 cut(s) 674
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 6 cut(s) 333, 724, 763, 915, 988, 1108
BstMAI GTCTC 5 cut(s) 321, 670, 998, 1033, 1045
BstMBI GATC 6 cut(s) 330, 721, 760, 912, 985, 1105
BstMWI GCNNNNNNNGC 3 cut(s) 107, 197, 1053
BstNI CCWGG 3 cut(s) 125, 474, 579
BstNSI RCATGY 1 cut(s) 645
BstSCI CCNGG 3 cut(s) 123, 472, 577
BstSFI CTRYAG 1 cut(s) 492
BstUI CGCG 1 cut(s) 674
BstV1I GCAGC 4 cut(s) 145, 795, 856, 1185
BstV2I GAAGAC 2 cut(s) 76, 126
BstX2I RGATCY 1 cut(s) 1105
BstYI RGATCY 1 cut(s) 1105
BstZ17I GTATAC 1 cut(s) 391
BsuRI GGCC 2 cut(s) 512, 648
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 4 cut(s) 439, 483, 514, 538
BtsIMutI CAGTG 1 cut(s) 15
Cac8I GCNNGC 1 cut(s) 1161
CaiI CAGNNNCTG 2 cut(s) 130, 1187
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 2 cut(s) 511, 523
CseI GACGC 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 123
Csp6I GTAC 2 cut(s) 379, 788
CviAII CATG 5 cut(s) 237, 445, 642, 1001, 1138
CviQI GTAC 2 cut(s) 379, 788
DdeI CTNAG 2 cut(s) 1165, 1188
DpnI GATC 6 cut(s) 332, 723, 762, 914, 987, 1107
DpnII GATC 6 cut(s) 330, 721, 760, 912, 985, 1105
DraI TTTAAA 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 237, 687
EarI CTCTTC 2 cut(s) 237, 687
Ecl136II GAGCTC 1 cut(s) 1171
Eco130I CCWWGG 2 cut(s) 397, 444
Eco24I GRGCYC 2 cut(s) 1072, 1173
Eco47I GGWCC 1 cut(s) 523
Eco53kI GAGCTC 1 cut(s) 1171
Eco57I CTGAAG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 1171
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoRII CCWGG 3 cut(s) 123, 472, 577
EcoT14I CCWWGG 2 cut(s) 397, 444
EcoT38I GRGCYC 2 cut(s) 1072, 1173
ErhI CCWWGG 2 cut(s) 397, 444
FaeI CATG 5 cut(s) 240, 448, 645, 1004, 1141
FatI CATG 5 cut(s) 236, 444, 641, 1000, 1137
FbaI TGATCA 1 cut(s) 985
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 4 cut(s) 159, 784, 870, 1199
FokI GGATG 4 cut(s) 446, 490, 501, 525
FriOI GRGCYC 2 cut(s) 1072, 1173
Fsp4HI GCNGC 4 cut(s) 159, 784, 870, 1199
FspBI CTAG 1 cut(s) 1118
GlaI GCGC 1 cut(s) 201
GluI GCNGC 4 cut(s) 159, 784, 870, 1199
GsaI CCCAGC 1 cut(s) 254
HaeIII GGCC 2 cut(s) 512, 648
HgaI GACGC 1 cut(s) 81
HhaI GCGC 1 cut(s) 202
Hin1II CATG 5 cut(s) 240, 448, 645, 1004, 1141
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 1 cut(s) 714
HindII GTYRAC 1 cut(s) 714
HindIII AAGCTT 2 cut(s) 15, 921
HinfI GANTC 7 cut(s) 233, 353, 740, 972, 1026, 1178, 1184
HphI GGTGA 2 cut(s) 770, 918
Hpy166II GTNNAC 5 cut(s) 35, 391, 707, 714, 769
Hpy188I TCNGA 4 cut(s) 106, 1168, 1183, 1189
Hpy188III TCNNGA 4 cut(s) 153, 370, 518, 542
Hpy8I GTNNAC 5 cut(s) 35, 391, 707, 714, 769
HpyAV CCTTC 4 cut(s) 131, 268, 332, 959
HpyCH4III ACNGT 6 cut(s) 10, 505, 711, 779, 835, 1010
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 8 cut(s) 4, 110, 305, 548, 793, 882, 894, 952
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 197, 1053
HpyF3I CTNAG 2 cut(s) 1165, 1188
HpySE526I ACGT 1 cut(s) 404
Hsp92II CATG 5 cut(s) 240, 448, 645, 1004, 1141
HspAI GCGC 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 985
Kzo9I GATC 6 cut(s) 330, 721, 760, 912, 985, 1105
LmnI GCTCC 2 cut(s) 551, 1099
Lsp1109I GCAGC 4 cut(s) 145, 795, 856, 1185
LweI GCATC 3 cut(s) 97, 110, 535
MabI ACCWGGT 1 cut(s) 123
MaeI CTAG 1 cut(s) 1118
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 439
MalI GATC 6 cut(s) 332, 723, 762, 914, 987, 1107
MboI GATC 6 cut(s) 330, 721, 760, 912, 985, 1105
MboII GAAGA 8 cut(s) 81, 131, 171, 254, 362, 704, 851, 1133
MflI RGATCY 1 cut(s) 1105
MhlI GDGCHC 2 cut(s) 1072, 1173
MluCI AATT 3 cut(s) 462, 528, 874
MlyI GAGTC 1 cut(s) 1035
MnlI CCTC 9 cut(s) 15, 216, 404, 514, 690, 730, 1051, 1119, 1174
MseI TTAA 5 cut(s) 218, 410, 621, 668, 938
MslI CAYNNNNRTG 1 cut(s) 543
MspR9I CCNGG 3 cut(s) 125, 474, 579
MvaI CCWGG 3 cut(s) 125, 474, 579
MvnI CGCG 1 cut(s) 674
MwoI GCNNNNNNNGC 3 cut(s) 107, 197, 1053
NcoI CCATGG 1 cut(s) 444
NdeII GATC 6 cut(s) 330, 721, 760, 912, 985, 1105
NlaIII CATG 5 cut(s) 240, 448, 645, 1004, 1141
NlaIV GGNNCC 1 cut(s) 1107
NmuCI GTSAC 1 cut(s) 439
NspI RCATGY 1 cut(s) 645
NspV TTCGAA 1 cut(s) 117
PfeI GAWTC 6 cut(s) 233, 353, 740, 972, 1178, 1184
PflMI CCANNNNNTGG 1 cut(s) 130
PkrI GCNGC 4 cut(s) 160, 785, 871, 1200
PleI GAGTC 1 cut(s) 1034
PpsI GAGTC 1 cut(s) 1034
Psp124BI GAGCTC 1 cut(s) 1173
Psp6I CCWGG 3 cut(s) 123, 472, 577
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 3 cut(s) 123, 472, 577
PspN4I GGNNCC 1 cut(s) 1107
PspPI GGNCC 2 cut(s) 511, 523
PstNI CAGNNNCTG 2 cut(s) 130, 1187
PsuI RGATCY 1 cut(s) 1105
RsaI GTAC 2 cut(s) 380, 789
RsaNI GTAC 2 cut(s) 379, 788
RseI CAYNNNNRTG 1 cut(s) 543
SacI GAGCTC 1 cut(s) 1173
SaqAI TTAA 5 cut(s) 218, 410, 621, 668, 938
SatI GCNGC 4 cut(s) 159, 784, 870, 1199
Sau3AI GATC 6 cut(s) 330, 721, 760, 912, 985, 1105
Sau96I GGNCC 2 cut(s) 511, 523
SchI GAGTC 1 cut(s) 1035
ScrFI CCNGG 3 cut(s) 125, 474, 579
SduI GDGCHC 2 cut(s) 1072, 1173
SexAI ACCWGGT 1 cut(s) 123
SfaNI GCATC 3 cut(s) 97, 110, 535
SfcI CTRYAG 1 cut(s) 492
SfuI TTCGAA 1 cut(s) 117
SinI GGWCC 1 cut(s) 523
SmiMI CAYNNNNRTG 1 cut(s) 543
Sse9I AATT 3 cut(s) 462, 528, 874
SsiI CCGC 2 cut(s) 99, 282
SspI AATATT 1 cut(s) 818
SspMI CTAG 1 cut(s) 1118
SstI GAGCTC 1 cut(s) 1173
StyD4I CCNGG 3 cut(s) 123, 472, 577
StyI CCWWGG 2 cut(s) 397, 444
TaaI ACNGT 6 cut(s) 10, 505, 711, 779, 835, 1010
TaiI ACGT 1 cut(s) 407
TaqI TCGA 6 cut(s) 117, 329, 369, 630, 1066, 1173
TaqII GACCGA 2 cut(s) 42, 731
TasI AATT 3 cut(s) 462, 528, 874
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 6 cut(s) 233, 353, 740, 972, 1178, 1184
Tru1I TTAA 5 cut(s) 218, 410, 621, 668, 938
Tru9I TTAA 5 cut(s) 218, 410, 621, 668, 938
TscAI CASTG 1 cut(s) 15
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 4 cut(s) 158, 783, 869, 1198
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 6 cut(s) 287, 351, 527, 879, 900, 1017
TspGWI ACGGA 1 cut(s) 672
TspRI CASTG 1 cut(s) 15
Van91I CCANNNNNTGG 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 523
XagI CCTNNNNNAGG 1 cut(s) 546
XapI RAATTY 1 cut(s) 462
XceI RCATGY 1 cut(s) 645
XmiI GTMKAC 1 cut(s) 390
XspI CTAG 1 cut(s) 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.