Rroxscaffold_7G00168690

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014122
Physical Location & Seq
Reverse (-)
9523275 .. 9527632
4358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00168690.1

Sequence Viewer

Length: 1224 bp
ATGCCCAACATCACCACCATGACCCCCAAGACCTGGTCACGGTTGAGGGGAGGGACCAAATCAAGAGCAGCCATTCTCAATAGACCATCAGCACTTAATGCCCTCAATACTGCTATGGTATCTCGCCTCAAGAGACTTTATGAATCATGGGAAGATAACCCGGATATTGGTTTTGTCCTGATGAAGGGTAATGGCAGAGCCTTCTGTTCTGGTGCGGATGCTGTTACCCTTTTTCAACTAGTCAATCAAGGGAAAGTTGAGGAATGCAGAAAATTTTTCGATACATTGTATAAGTTTGTATATATTCAAGGAACATACTTGAAGCCACATGTAGCTATCTTGGATGGTATAACTATGGGAGGTGGGGCTGGAATTTCACTACCAGGAATGTTTCGTGTGGTGACTGATAAAACTGTTTTTTGCAATCCAGAGGCTCAAATAGGTTTCCATCCTGATGCAGGAGCTTCATATTATCTTTCTCGTCTACCTGGCTACTTAGGAGAATACTTAGCTCTTACAGGGGACAAGCTTAATGGTGTAGAGATGATTGCATGTGGCCTTGCCACTCATTATGTATTAACTGCAAGACTTGCTATGGTTGAAGAACGCGTGGGTAAATTGGTCACAGATGAACCATCTGTTATAAAAGCATCTCTTGCAGAGTATGGTGACCTTGTTAATCTAGACCGGAAGAGTATACTTGCTAAGCTTGAAGGGATTGACAGATGTTTCAGCCATGACACGGTTGAGGAAATTATTGATGCTTTGGAAAAGGAAGCTGCTGATTCTTATGATGAATGGTGCACCACAGCCCTCAAGAAACTAAAAGATGCCTCCCCTTTGAGTTTGAAAGTTTCCCTACGATCTATACGTGAAGGCAGATTTGAATCTCTTCATCAATGTCTGGCTCGTGAATACCGGATATCCCTAAATGGAATTTCCAAACGGGTCTCAAGTGACTTCTGTGAGGGTGTTTGGGCGAGATTAGTGGAAAAGGACTTTGCACCCAAGTGGGACCCTCCTAGTTTGAAAGAAGTGTCTAAAGACATGGTTGACTGCTATTTCTCTCCGCTCCCTGAAGTTGTGCCTGAGCTGGAGTTGCCAAACCGCGTCGCGAGAGCCATTTATGTAGGAGTCATTCACACACAAGAGGAGACTGAATTTTGTGCAATGCTTGATGAAGAAAAACAAGAAGAAACTTGTGCTTTGTCGATTGTGAGCTAG

Protein Analysis

407

Amino Acids

45.25

Weight (kDa)

5.5

Isoelectric Point (pI)

29.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 22 - 355 4.3e-116 Enoyl-CoA hydratase/isomerase
ECH_1 PF00378 26 - 210 5.8e-21 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014122)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31810
fragaria_vesca FvH4_2g41110
malus_domestica MD08G1057200.v1.1 MD15G1040200.v1.1
prunus_persica Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1
pyrus_communis pycom08g04670
rosa_chinensis RchiOBHm_Chr6g0299431
rosa_laevigata RLG00000011432
rosa_multiflora Rmu_sc0037301.1_g000004
rosa_roxburghii Rroxscaffold_7G00168690
rosa_rugosa Rorug06G0288100
rosa_samantha Rh6AG400000 Rh6BG408600 Rh6CG414100
rosa_wichuraiana Rw6G035010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AccB7I CCANNNNNTGG 1 cut(s) 33
AccBSI CCGCTC 1 cut(s) 1072
AccI GTMKAC 2 cut(s) 484, 697
AccII CGCG 3 cut(s) 609, 1110, 1115
AciI CCGC 3 cut(s) 215, 1070, 1108
AcsI RAATTY 4 cut(s) 272, 372, 936, 1160
AcuI CTGAAG 1 cut(s) 1098
AfiI CCNNNNNNNGG 6 cut(s) 33, 39, 167, 184, 458, 742
AflIII ACRYGT 2 cut(s) 328, 607
AgsI TTSAA 8 cut(s) 236, 308, 322, 602, 713, 850, 887, 1030
AhlI ACTAGT 1 cut(s) 238
AjnI CCWGG 3 cut(s) 32, 382, 487
AleI CACNNNNGTG 1 cut(s) 1009
AluBI AGCT 8 cut(s) 335, 464, 512, 529, 709, 779, 1093, 1221
AluI AGCT 8 cut(s) 335, 464, 512, 529, 709, 779, 1093, 1221
Alw21I GWGCWC 1 cut(s) 806
Alw26I GTCTC 3 cut(s) 127, 955, 1148
Alw44I GTGCAC 1 cut(s) 802
AoxI GGCC 1 cut(s) 556
ApaLI GTGCAC 1 cut(s) 802
ApeKI GCWGC 2 cut(s) 68, 779
ApoI RAATTY 4 cut(s) 272, 372, 936, 1160
Asp700I GAANNNNTTC 1 cut(s) 891
AspS9I GGNCC 2 cut(s) 54, 1015
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 3 cut(s) 4, 412, 680
AvaII GGWCC 2 cut(s) 54, 1015
BaeGI GKGCMC 1 cut(s) 806
BauI CACGAG 1 cut(s) 909
Bbv12I GWGCWC 1 cut(s) 806
BbvI GCAGC 2 cut(s) 80, 766
BccI CCATC 4 cut(s) 94, 338, 456, 643
BciT130I CCWGG 3 cut(s) 34, 384, 489
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 3 cut(s) 127, 955, 1148
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 4 cut(s) 239, 683, 1023, 1222
BisI GCNGC 2 cut(s) 69, 780
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 2 cut(s) 70, 781
Bme1390I CCNGG 4 cut(s) 34, 161, 384, 489
Bme18I GGWCC 2 cut(s) 54, 1015
BmgT120I GGNCC 2 cut(s) 54, 1015
BmiI GGNNCC 3 cut(s) 55, 1016, 1017
BmrFI CCNGG 4 cut(s) 34, 161, 384, 489
BmsI GCATC 5 cut(s) 208, 445, 659, 751, 820
BpmI CTGGAG 1 cut(s) 1115
Bpu10I CCTNAGC 1 cut(s) 1089
Bpu1102I GCTNAGC 1 cut(s) 705
BpuEI CTTGAG 3 cut(s) 113, 800, 937
BpuMI CCSGG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 872
BsaBI GATNNNNATC 1 cut(s) 886
BsaI GGTCTC 1 cut(s) 955
BsaWI WCCGGW 2 cut(s) 687, 918
Bsc4I CCNNNNNNNGG 6 cut(s) 33, 39, 167, 184, 458, 742
Bse3DI GCAATG 1 cut(s) 1176
Bse8I GATNNNNATC 1 cut(s) 886
BseBI CCWGG 3 cut(s) 34, 384, 489
BseGI GGATG 3 cut(s) 223, 349, 448
BseJI GATNNNNATC 1 cut(s) 886
BseLI CCNNNNNNNGG 6 cut(s) 33, 39, 167, 184, 458, 742
BseMI GCAATG 1 cut(s) 1176
BseMII CTCAG 1 cut(s) 1080
BseRI GAGGAG 1 cut(s) 1166
BseSI GKGCMC 1 cut(s) 806
BseXI GCAGC 2 cut(s) 80, 766
Bsh1236I CGCG 3 cut(s) 609, 1110, 1115
BshFI GGCC 1 cut(s) 558
BsiHKAI GWGCWC 1 cut(s) 806
BsiSI CCGG 3 cut(s) 161, 688, 919
BslFI GGGAC 3 cut(s) 67, 536, 1028
BslI CCNNNNNNNGG 6 cut(s) 33, 39, 167, 184, 458, 742
BsmAI GTCTC 3 cut(s) 127, 955, 1148
BsmFI GGGAC 3 cut(s) 67, 536, 1028
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 1 cut(s) 558
Bso31I GGTCTC 1 cut(s) 955
Bsp1286I GDGCHC 1 cut(s) 806
Bsp143I GATC 1 cut(s) 863
Bsp1720I GCTNAGC 1 cut(s) 705
Bsp68I TCGCGA 1 cut(s) 1115
BspACI CCGC 3 cut(s) 215, 1070, 1108
BspANI GGCC 1 cut(s) 558
BspCNI CTCAG 1 cut(s) 1081
BspFNI CGCG 3 cut(s) 609, 1110, 1115
BspLI GGNNCC 3 cut(s) 55, 1016, 1017
BspTNI GGTCTC 1 cut(s) 955
BsrBI CCGCTC 1 cut(s) 1072
BsrDI GCAATG 1 cut(s) 1176
BssMI GATC 1 cut(s) 863
BssNAI GTATAC 1 cut(s) 698
BssSI CACGAG 1 cut(s) 909
Bst1107I GTATAC 1 cut(s) 698
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 3 cut(s) 34, 384, 489
Bst4CI ACNGT 3 cut(s) 42, 415, 745
Bst6I CTCTTC 2 cut(s) 686, 897
BstAPI GCANNNNNTGC 3 cut(s) 98, 590, 656
BstBAI YACGTR 1 cut(s) 872
BstDEI CTNAG 4 cut(s) 496, 508, 705, 1089
BstEII GGTNACC 1 cut(s) 668
BstENI CCTNNNNNAGG 2 cut(s) 182, 456
BstF5I GGATG 3 cut(s) 223, 349, 448
BstFNI CGCG 3 cut(s) 609, 1110, 1115
BstKTI GATC 1 cut(s) 866
BstMAI GTCTC 3 cut(s) 127, 955, 1148
BstMBI GATC 1 cut(s) 863
BstMWI GCNNNNNNNGC 4 cut(s) 98, 590, 656, 1099
BstNI CCWGG 3 cut(s) 34, 384, 489
BstNSI RCATGY 2 cut(s) 332, 555
BstPI GGTNACC 1 cut(s) 668
BstSCI CCNGG 4 cut(s) 32, 159, 382, 487
BstSLI GKGCMC 1 cut(s) 806
BstUI CGCG 3 cut(s) 609, 1110, 1115
BstV1I GCAGC 2 cut(s) 80, 766
BstZ17I GTATAC 1 cut(s) 698
BsuRI GGCC 1 cut(s) 558
BtsCI GGATG 3 cut(s) 223, 349, 448
BtuMI TCGCGA 1 cut(s) 1115
Cfr13I GGNCC 2 cut(s) 54, 1015
CseI GACGC 1 cut(s) 1099
CsiI ACCWGGT 1 cut(s) 32
CviAII CATG 6 cut(s) 19, 147, 329, 552, 737, 1048
DdeI CTNAG 4 cut(s) 496, 508, 705, 1089
DpnI GATC 1 cut(s) 865
DpnII GATC 1 cut(s) 863
Eam1104I CTCTTC 2 cut(s) 686, 897
EarI CTCTTC 2 cut(s) 686, 897
Eco31I GGTCTC 1 cut(s) 955
Eco32I GATATC 1 cut(s) 924
Eco47I GGWCC 2 cut(s) 54, 1015
Eco57I CTGAAG 1 cut(s) 1098
Eco91I GGTNACC 1 cut(s) 668
EcoNI CCTNNNNNAGG 2 cut(s) 182, 456
EcoO109I RGGNCCY 1 cut(s) 1015
EcoO65I GGTNACC 1 cut(s) 668
EcoRII CCWGG 3 cut(s) 32, 382, 487
EcoRV GATATC 1 cut(s) 924
FaeI CATG 6 cut(s) 22, 150, 332, 555, 740, 1051
FalI AAGNNNNNCTT 2 cut(s) 639, 671
FaqI GGGAC 3 cut(s) 67, 536, 1028
FatI CATG 6 cut(s) 18, 146, 328, 551, 736, 1047
FblI GTMKAC 2 cut(s) 484, 697
Fnu4HI GCNGC 2 cut(s) 69, 780
FokI GGATG 3 cut(s) 230, 356, 435
Fsp4HI GCNGC 2 cut(s) 69, 780
FspBI CTAG 4 cut(s) 239, 683, 1023, 1222
GluI GCNGC 2 cut(s) 69, 780
GsuI CTGGAG 1 cut(s) 1115
HaeIII GGCC 1 cut(s) 558
HapII CCGG 3 cut(s) 161, 688, 919
HgaI GACGC 1 cut(s) 1099
Hin1II CATG 6 cut(s) 22, 150, 332, 555, 740, 1051
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 2 cut(s) 527, 707
HinfI GANTC 4 cut(s) 143, 785, 887, 1134
HpaII CCGG 3 cut(s) 161, 688, 919
HphI GGTGA 3 cut(s) 4, 412, 680
Hpy166II GTNNAC 4 cut(s) 485, 698, 804, 1054
Hpy188III TCNNGA 9 cut(s) 63, 130, 178, 428, 452, 683, 817, 911, 1114
Hpy8I GTNNAC 4 cut(s) 485, 698, 804, 1054
Hpy99I CGWCG 1 cut(s) 1115
HpyAV CCTTC 4 cut(s) 178, 211, 707, 869
HpyCH4III ACNGT 3 cut(s) 42, 415, 745
HpyCH4IV ACGT 1 cut(s) 871
HpyCH4V TGCA 9 cut(s) 267, 423, 458, 551, 584, 659, 804, 1004, 1169
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 590, 656, 1099
HpyF3I CTNAG 4 cut(s) 496, 508, 705, 1089
HpySE526I ACGT 1 cut(s) 871
Hsp92II CATG 6 cut(s) 22, 150, 332, 555, 740, 1051
KflI GGGWCCC 1 cut(s) 1015
Kzo9I GATC 1 cut(s) 863
LmnI GCTCC 2 cut(s) 461, 1077
Lsp1109I GCAGC 2 cut(s) 80, 766
LweI GCATC 5 cut(s) 208, 445, 659, 751, 820
MabI ACCWGGT 1 cut(s) 32
MaeI CTAG 4 cut(s) 239, 683, 1023, 1222
MaeII ACGT 1 cut(s) 871
MaeIII GTNAC 6 cut(s) 36, 223, 400, 622, 668, 956
MalI GATC 1 cut(s) 865
MbiI CCGCTC 1 cut(s) 1072
MboI GATC 1 cut(s) 863
MboII GAAGA 6 cut(s) 164, 614, 703, 884, 1193, 1205
MhlI GDGCHC 1 cut(s) 806
MluCI AATT 6 cut(s) 272, 372, 617, 753, 936, 1160
MluI ACGCGT 1 cut(s) 607
MlyI GAGTC 1 cut(s) 1143
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 4 cut(s) 96, 531, 578, 678
MslI CAYNNNNRTG 3 cut(s) 17, 453, 1009
MspI CCGG 3 cut(s) 161, 688, 919
MspR9I CCNGG 4 cut(s) 34, 161, 384, 489
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 3 cut(s) 34, 384, 489
MvnI CGCG 3 cut(s) 609, 1110, 1115
MwoI GCNNNNNNNGC 4 cut(s) 98, 590, 656, 1099
NciI CCSGG 1 cut(s) 161
NdeII GATC 1 cut(s) 863
NlaIII CATG 6 cut(s) 22, 150, 332, 555, 740, 1051
NlaIV GGNNCC 3 cut(s) 55, 1016, 1017
NmuCI GTSAC 5 cut(s) 36, 400, 622, 668, 956
NruI TCGCGA 1 cut(s) 1115
NspI RCATGY 2 cut(s) 332, 555
OliI CACNNNNGTG 1 cut(s) 1009
PciI ACATGT 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 868
PctI GAATGC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 3 cut(s) 143, 785, 887
PflFI GACNNNGTC 1 cut(s) 34
PflMI CCANNNNNTGG 1 cut(s) 33
PkrI GCNGC 2 cut(s) 70, 781
PleI GAGTC 1 cut(s) 1142
PpsI GAGTC 1 cut(s) 1142
Ppu21I YACGTR 1 cut(s) 872
PpuMI RGGWCCY 1 cut(s) 1015
PscI ACATGT 1 cut(s) 328
PsiI TTATAA 1 cut(s) 644
Psp5II RGGWCCY 1 cut(s) 1015
Psp6I CCWGG 3 cut(s) 32, 382, 487
PspEI GGTNACC 1 cut(s) 668
PspGI CCWGG 3 cut(s) 32, 382, 487
PspN4I GGNNCC 3 cut(s) 55, 1016, 1017
PspPI GGNCC 2 cut(s) 54, 1015
PspPPI RGGWCCY 1 cut(s) 1015
PsyI GACNNNGTC 1 cut(s) 34
RruI TCGCGA 1 cut(s) 1115
RseI CAYNNNNRTG 3 cut(s) 17, 453, 1009
SaqAI TTAA 4 cut(s) 96, 531, 578, 678
SatI GCNGC 2 cut(s) 69, 780
Sau3AI GATC 1 cut(s) 863
Sau96I GGNCC 2 cut(s) 54, 1015
SchI GAGTC 1 cut(s) 1143
ScrFI CCNGG 4 cut(s) 34, 161, 384, 489
SduI GDGCHC 1 cut(s) 806
SexAI ACCWGGT 1 cut(s) 32
SfaNI GCATC 5 cut(s) 208, 445, 659, 751, 820
SinI GGWCC 2 cut(s) 54, 1015
SmiMI CAYNNNNRTG 3 cut(s) 17, 453, 1009
SmlI CTYRAG 3 cut(s) 128, 815, 952
SmoI CTYRAG 3 cut(s) 128, 815, 952
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 6 cut(s) 272, 372, 617, 753, 936, 1160
SsiI CCGC 3 cut(s) 215, 1070, 1108
SspMI CTAG 4 cut(s) 239, 683, 1023, 1222
StyD4I CCNGG 4 cut(s) 32, 159, 382, 487
TaaI ACNGT 3 cut(s) 42, 415, 745
TaiI ACGT 1 cut(s) 874
TaqI TCGA 2 cut(s) 279, 1211
TasI AATT 6 cut(s) 272, 372, 617, 753, 936, 1160
TfiI GAWTC 3 cut(s) 143, 785, 887
Tru1I TTAA 4 cut(s) 96, 531, 578, 678
Tru9I TTAA 4 cut(s) 96, 531, 578, 678
TseFI GTSAC 5 cut(s) 36, 400, 622, 668, 956
TseI GCWGC 2 cut(s) 68, 779
Tsp45I GTSAC 5 cut(s) 36, 400, 622, 668, 956
TspDTI ATGAA 7 cut(s) 156, 197, 456, 645, 810, 884, 1194
Tth111I GACNNNGTC 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 33
VneI GTGCAC 1 cut(s) 802
VpaK11BI GGWCC 2 cut(s) 54, 1015
XagI CCTNNNNNAGG 2 cut(s) 182, 456
XapI RAATTY 4 cut(s) 272, 372, 936, 1160
XbaI TCTAGA 1 cut(s) 682
XceI RCATGY 2 cut(s) 332, 555
XmiI GTMKAC 2 cut(s) 484, 697
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 4 cut(s) 239, 683, 1023, 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.