Rorug06G0288100

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014122
Physical Location & Seq
Reverse (-)
43779441 .. 43780493
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0288100.1

Sequence Viewer

Length: 669 bp
ATGGAAGAAGTGAAGCTACTAGGAACATGGCCTAGTCCCTTTTGTTACAGGGTCATATGGGCTCTGAAACTGAAGGGTGTGAAGTATGAAGATGTGGAAGAGGACCTTGTCAACAAGAGTGATCGTCTTTTACAGTACAACCCAGTTCATAAGAAGGTACCGGTGCTCGTTCATGGTGGAAAACCGATCTTAGAGTCTACTGTTATTCTTGAATACATTGAGGAGGTGTGGCCACAGAATCCCTTGCTTTCTGATGACCCTTATGAGAGGGCCACGGCTCGGTTTTGGACTAAATTTGGAGCGGACAAGAGCCCCATTTTTATTAAATTTTTTCATACAGTTGGGGAAGAGCATGAGAGGGCAAGAAAAGAGGGACAAGAAGTGCTGAAAATAATGGAAGAACAAGGGCTTGGAGAAAAGAAATTTTTTGGAGGGGACAAAGTTGGACTGGCAGACTTGGCATTTGGATGGATAGCTGGTTGGCTTCAAGCGATGGAAGAAGCAGCTGGTGTGAAGCTTTTAGAAGCCAACATGTTTCCTCGCTTGGAGGATTGGATCAAGAATTTCAGAGAAGTCACTGTGATCAAGGACAACCTTCCAGGTCATGAAGAATTGTTGGACTATTTCAGGCGCCTAAGAGAGAGGCTTGCTCTATTATCAACGTCGTAG

Protein Analysis

222

Amino Acids

25.73

Weight (kDa)

5.53

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 3 - 75 1.2e-19 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 75 5.3e-16 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 12 - 79 6.5e-15 Glutathione S-transferase, N-terminal domain
GST_C PF00043 112 - 190 2e-07 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 117 - 187 1.1e-06 Glutathione S-transferase, C-terminal domain
DUF7962 PF25907 122 - 193 5.7e-06 Domain of unknown function (DUF7962)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014122)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31810
fragaria_vesca FvH4_2g41110
malus_domestica MD08G1057200.v1.1 MD15G1040200.v1.1
prunus_persica Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1
pyrus_communis pycom08g04670
rosa_chinensis RchiOBHm_Chr6g0299431
rosa_laevigata RLG00000011432
rosa_multiflora Rmu_sc0037301.1_g000004
rosa_roxburghii Rroxscaffold_7G00168690
rosa_rugosa Rorug06G0288100
rosa_samantha Rh6AG400000 Rh6BG408600 Rh6CG414100
rosa_wichuraiana Rw6G035010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 157
AccB1I GGYRCC 2 cut(s) 157, 630
AccBSI CCGCTC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 197
AciI CCGC 1 cut(s) 302
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 230
AcsI RAATTY 4 cut(s) 293, 326, 422, 562
AcuI CTGAAG 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 631
AfaI GTAC 2 cut(s) 137, 159
AfiI CCNNNNNNNGG 1 cut(s) 279
AflIII ACRYGT 1 cut(s) 531
AgeI ACCGGT 1 cut(s) 160
AgsI TTSAA 2 cut(s) 212, 488
AjnI CCWGG 1 cut(s) 598
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
AluBI AGCT 4 cut(s) 16, 476, 506, 517
AluI AGCT 4 cut(s) 16, 476, 506, 517
Alw21I GWGCWC 1 cut(s) 168
AlwI GGATC 1 cut(s) 563
AoxI GGCC 3 cut(s) 29, 230, 270
ApeKI GCWGC 1 cut(s) 503
ApoI RAATTY 4 cut(s) 293, 326, 422, 562
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
AsiGI ACCGGT 1 cut(s) 160
Asp718I GGTACC 1 cut(s) 157
AspLEI GCGC 1 cut(s) 633
AspS9I GGNCC 2 cut(s) 103, 270
AvaII GGWCC 1 cut(s) 103
BalI TGGCCA 1 cut(s) 232
BanI GGYRCC 2 cut(s) 157, 630
BanII GRGCYC 2 cut(s) 64, 314
BarI GAAGNNNNNNTAC 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 515
BccI CCATC 2 cut(s) 462, 487
BceAI ACGGC 1 cut(s) 291
BciT130I CCWGG 1 cut(s) 600
BclI TGATCA 1 cut(s) 582
BfaI CTAG 2 cut(s) 20, 33
BfoI RGCGCY 1 cut(s) 634
BisI GCNGC 1 cut(s) 504
BlsI GCNGC 1 cut(s) 505
Bme1390I CCNGG 1 cut(s) 600
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 103, 270
BmiI GGNNCC 2 cut(s) 159, 632
BmrFI CCNGG 1 cut(s) 600
BmrI ACTGGG 1 cut(s) 137
BmuI ACTGGG 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 634, 666
BsaHI GRCGYC 1 cut(s) 631
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 143, 453
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 473
BseLI CCNNNNNNNGG 1 cut(s) 279
BseNI ACTGG 2 cut(s) 143, 453
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 1 cut(s) 515
BshFI GGCC 3 cut(s) 31, 232, 272
BshNI GGYRCC 2 cut(s) 157, 630
BshTI ACCGGT 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 3 cut(s) 21, 387, 449
BslI CCNNNNNNNGG 1 cut(s) 279
BsmFI GGGAC 3 cut(s) 21, 387, 449
BsnI GGCC 3 cut(s) 31, 232, 272
Bsp1286I GDGCHC 3 cut(s) 64, 168, 314
Bsp143I GATC 4 cut(s) 121, 186, 555, 582
BspACI CCGC 1 cut(s) 302
BspANI GGCC 3 cut(s) 31, 232, 272
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 159, 632
BspPI GGATC 1 cut(s) 563
BspQI GCTCTTC 1 cut(s) 342
BspT107I GGYRCC 2 cut(s) 157, 630
BsrBI CCGCTC 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 2 cut(s) 143, 453
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 4 cut(s) 121, 186, 555, 582
BssNI GRCGYC 1 cut(s) 631
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 4 cut(s) 135, 202, 340, 580
Bst6I CTCTTC 2 cut(s) 93, 342
BstACI GRCGYC 1 cut(s) 631
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 2 cut(s) 190, 635
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 473
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 1 cut(s) 633
BstKTI GATC 4 cut(s) 124, 189, 558, 585
BstMBI GATC 4 cut(s) 121, 186, 555, 582
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 1 cut(s) 600
BstNSI RCATGY 1 cut(s) 535
BstSCI CCNGG 1 cut(s) 598
BstV1I GCAGC 1 cut(s) 515
BsuRI GGCC 3 cut(s) 31, 232, 272
BtgI CCRYGG 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 506
BtsCI GGATG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 576
Cac8I GCNNGC 1 cut(s) 648
CciI TCATGA 1 cut(s) 604
CfoI GCGC 1 cut(s) 633
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 103, 270
Csp6I GTAC 2 cut(s) 136, 158
CspAI ACCGGT 1 cut(s) 160
CviAII CATG 5 cut(s) 27, 173, 353, 532, 605
CviQI GTAC 2 cut(s) 136, 158
DdeI CTNAG 2 cut(s) 190, 635
DinI GGCGCC 1 cut(s) 632
DpnI GATC 4 cut(s) 123, 188, 557, 584
DpnII GATC 4 cut(s) 121, 186, 555, 582
EaeI YGGCCR 1 cut(s) 230
Eam1104I CTCTTC 2 cut(s) 93, 342
EarI CTCTTC 2 cut(s) 93, 342
Eco24I GRGCYC 2 cut(s) 64, 314
Eco47I GGWCC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 598
EcoT38I GRGCYC 2 cut(s) 64, 314
EgeI GGCGCC 1 cut(s) 632
EheI GGCGCC 1 cut(s) 632
FaeI CATG 5 cut(s) 30, 176, 356, 535, 608
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FaqI GGGAC 3 cut(s) 21, 387, 449
FatI CATG 5 cut(s) 26, 172, 352, 531, 604
FauNDI CATATG 1 cut(s) 56
FbaI TGATCA 1 cut(s) 582
FblI GTMKAC 1 cut(s) 197
Fnu4HI GCNGC 1 cut(s) 504
FokI GGATG 1 cut(s) 480
FriOI GRGCYC 2 cut(s) 64, 314
Fsp4HI GCNGC 1 cut(s) 504
FspBI CTAG 2 cut(s) 20, 33
GlaI GCGC 1 cut(s) 632
GluI GCNGC 1 cut(s) 504
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 3 cut(s) 31, 232, 272
HapII CCGG 1 cut(s) 161
HhaI GCGC 1 cut(s) 633
Hin1I GRCGYC 1 cut(s) 631
Hin1II CATG 5 cut(s) 30, 176, 356, 535, 608
Hin6I GCGC 1 cut(s) 631
HinP1I GCGC 1 cut(s) 631
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 2 cut(s) 194, 238
HpaII CCGG 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 112, 198
Hpy188I TCNGA 3 cut(s) 66, 253, 569
Hpy188III TCNNGA 3 cut(s) 209, 559, 605
Hpy8I GTNNAC 2 cut(s) 112, 198
Hpy99I CGWCG 1 cut(s) 667
HpyAV CCTTC 3 cut(s) 67, 148, 605
HpyCH4III ACNGT 4 cut(s) 135, 202, 340, 580
HpyCH4IV ACGT 1 cut(s) 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 2 cut(s) 190, 635
HpySE526I ACGT 1 cut(s) 662
Hsp92I GRCGYC 1 cut(s) 631
Hsp92II CATG 5 cut(s) 30, 176, 356, 535, 608
HspAI GCGC 1 cut(s) 631
KasI GGCGCC 1 cut(s) 630
KpnI GGTACC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 4 cut(s) 121, 186, 555, 582
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 9 cut(s) 34, 156, 174, 434, 462, 492, 585, 612, 613
Lsp1109I GCAGC 1 cut(s) 515
MaeI CTAG 2 cut(s) 20, 33
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 2 cut(s) 44, 574
MalI GATC 4 cut(s) 123, 188, 557, 584
MbiI CCGCTC 1 cut(s) 302
MboI GATC 4 cut(s) 121, 186, 555, 582
MboII GAAGA 7 cut(s) 17, 101, 110, 359, 410, 509, 620
MhlI GDGCHC 3 cut(s) 64, 168, 314
MlsI TGGCCA 1 cut(s) 232
MluCI AATT 5 cut(s) 293, 326, 422, 562, 611
MluNI TGGCCA 1 cut(s) 232
Mly113I GGCGCC 1 cut(s) 631
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 2 cut(s) 424, 597
Mox20I TGGCCA 1 cut(s) 232
MscI TGGCCA 1 cut(s) 232
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 466
Msp20I TGGCCA 1 cut(s) 232
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 600
MvaI CCWGG 1 cut(s) 600
MwoI GCNNNNNNNGC 1 cut(s) 458
NarI GGCGCC 1 cut(s) 631
NdeI CATATG 1 cut(s) 56
NdeII GATC 4 cut(s) 121, 186, 555, 582
NlaIII CATG 5 cut(s) 30, 176, 356, 535, 608
NlaIV GGNNCC 2 cut(s) 159, 632
NmuCI GTSAC 1 cut(s) 574
NspI RCATGY 1 cut(s) 535
PagI TCATGA 1 cut(s) 604
PciI ACATGT 1 cut(s) 531
PciSI GCTCTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 238
PflFI GACNNNGTC 1 cut(s) 107
PinAI ACCGGT 1 cut(s) 160
PkrI GCNGC 1 cut(s) 505
PleI GAGTC 1 cut(s) 202
PluTI GGCGCC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 202
PpuMI RGGWCCY 1 cut(s) 103
PscI ACATGT 1 cut(s) 531
Psp5II RGGWCCY 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 2 cut(s) 159, 632
PspPI GGNCC 2 cut(s) 103, 270
PspPPI RGGWCCY 1 cut(s) 103
PsyI GACNNNGTC 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 506
RsaI GTAC 2 cut(s) 137, 159
RsaNI GTAC 2 cut(s) 136, 158
RseI CAYNNNNRTG 1 cut(s) 466
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 4 cut(s) 121, 186, 555, 582
Sau96I GGNCC 2 cut(s) 103, 270
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 600
SduI GDGCHC 3 cut(s) 64, 168, 314
SfoI GGCGCC 1 cut(s) 632
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 466
Sse9I AATT 5 cut(s) 293, 326, 422, 562, 611
SsiI CCGC 1 cut(s) 302
SspDI GGCGCC 1 cut(s) 630
SspMI CTAG 2 cut(s) 20, 33
StyD4I CCNGG 1 cut(s) 598
TaaI ACNGT 4 cut(s) 135, 202, 340, 580
TaiI ACGT 1 cut(s) 665
TasI AATT 5 cut(s) 293, 326, 422, 562, 611
TatI WGTACW 1 cut(s) 135
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 1 cut(s) 574
TseI GCWGC 1 cut(s) 503
Tsp45I GTSAC 1 cut(s) 574
TspDTI ATGAA 5 cut(s) 102, 137, 161, 323, 621
TspRI CASTG 1 cut(s) 583
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 103
XapI RAATTY 4 cut(s) 293, 326, 422, 562
XceI RCATGY 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 197
XspI CTAG 2 cut(s) 20, 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.