Rh6BG408600

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0014122
Physical Location & Seq
Forward (+)
63430670 .. 63437508
6839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG408600.1

Sequence Viewer

Length: 807 bp
ATGCAGGTAGCTATCTTGGATGGTATAACTATGGGAGGTGGGGCTGGGATTTCACTCCCAGGAATGTTTCGTGTGGTGACTGATAAAACTGTTTTTTGCAATCCAGAGGCTCAAATAGGTTTCCATCCTGATGCAGGAGCTTCATATTATCTTTCTCGTCTACCTGGCTACTTAGGAGAATACTTAGCTCTTACAGGGGACAAGCTTAATGGTGTAGAGATGATTGCATGTGGCCTTGCCACTCATTATGTATTAAATGCAAGACTTGCTATGGTTGAAGAACGCGTGGGTAAATTGGTCACAGATGAACCATCTGTTATAAAAGCATCTCTTGCAGAGTATGGTGACCTTGTTAATCTAGACCAGAAGAGTATACTTGCTAAGCTTGAAGGGATTGACAGATGTTTCAGCCATGACACGGTTGAGGAAATTATTGATGCTTTGGAAAAGGAAGCTGCTGATTCTTATGATGAATGGTGCACCACAGCCCTCAAGAAACTAAAAGAAGCCTCCCCTTTGAGTTTGAAAGTTTCCCTACGATCTATACGTGAAGGCAGATTTGAATCTCTTCATCAATGTCTGGCTCGTGAATACCGGATATCCCTAAATGGAATTTCCAAACGGGTCTCAAGTGACTTCTGTGAGGGTGTTTGGGCGAGATTAGTGGAAAAGGACTTTGCACCCAAGTGGGACCCTCCTAGTTTGAAAGAAGTGTCTAAAGACATGGTTGACTGCTATTTCTCTCCGCTCCCTGAAGTTGAGCCTGAGCTGGAGTTGCCAACCGCGTTGCGAGAGCCATTTATGTAG

Protein Analysis

268

Amino Acids

29.64

Weight (kDa)

4.86

Isoelectric Point (pI)

28.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 2 - 247 1e-85 Enoyl-CoA hydratase/isomerase
ECH_1 PF00378 3 - 113 8e-08 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014122)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31810
fragaria_vesca FvH4_2g41110
malus_domestica MD08G1057200.v1.1 MD15G1040200.v1.1
prunus_persica Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1
pyrus_communis pycom08g04670
rosa_chinensis RchiOBHm_Chr6g0299431
rosa_laevigata RLG00000011432
rosa_multiflora Rmu_sc0037301.1_g000004
rosa_roxburghii Rroxscaffold_7G00168690
rosa_rugosa Rorug06G0288100
rosa_samantha Rh6AG400000 Rh6BG408600 Rh6CG414100
rosa_wichuraiana Rw6G035010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 320
AccBSI CCGCTC 1 cut(s) 748
AccI GTMKAC 2 cut(s) 160, 373
AccII CGCG 2 cut(s) 285, 785
AciI CCGC 2 cut(s) 746, 783
AcsI RAATTY 1 cut(s) 612
AcuI CTGAAG 1 cut(s) 774
AfiI CCNNNNNNNGG 2 cut(s) 134, 418
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 5 cut(s) 278, 389, 526, 563, 706
AjnI CCWGG 2 cut(s) 58, 163
AleI CACNNNNGTG 1 cut(s) 685
AluBI AGCT 7 cut(s) 11, 140, 188, 205, 385, 455, 769
AluI AGCT 7 cut(s) 11, 140, 188, 205, 385, 455, 769
Alw21I GWGCWC 1 cut(s) 482
Alw26I GTCTC 1 cut(s) 631
Alw44I GTGCAC 1 cut(s) 478
AoxI GGCC 1 cut(s) 232
ApaLI GTGCAC 1 cut(s) 478
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 567
AspS9I GGNCC 1 cut(s) 691
AsuHPI GGTGA 2 cut(s) 88, 356
AvaII GGWCC 1 cut(s) 691
BaeGI GKGCMC 1 cut(s) 482
BauI CACGAG 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 482
BbvI GCAGC 1 cut(s) 442
BccI CCATC 3 cut(s) 14, 132, 319
BciT130I CCWGG 2 cut(s) 60, 165
BcoDI GTCTC 1 cut(s) 631
BfaI CTAG 2 cut(s) 359, 699
BisI GCNGC 1 cut(s) 456
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 457
Bme1390I CCNGG 2 cut(s) 60, 165
Bme18I GGWCC 1 cut(s) 691
BmgT120I GGNCC 1 cut(s) 691
BmiI GGNNCC 2 cut(s) 692, 693
BmrFI CCNGG 2 cut(s) 60, 165
BmsI GCATC 3 cut(s) 121, 335, 427
BpmI CTGGAG 1 cut(s) 791
Bpu10I CCTNAGC 1 cut(s) 765
Bpu1102I GCTNAGC 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 476, 613
BsaAI YACGTR 1 cut(s) 548
BsaBI GATNNNNATC 1 cut(s) 562
BsaI GGTCTC 1 cut(s) 631
BsaJI CCNNGG 1 cut(s) 58
BsaWI WCCGGW 1 cut(s) 594
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 418
Bse8I GATNNNNATC 1 cut(s) 562
BseBI CCWGG 2 cut(s) 60, 165
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 2 cut(s) 25, 124
BseJI GATNNNNATC 1 cut(s) 562
BseLI CCNNNNNNNGG 2 cut(s) 134, 418
BseMII CTCAG 1 cut(s) 756
BseSI GKGCMC 1 cut(s) 482
BseXI GCAGC 1 cut(s) 442
BseYI CCCAGC 1 cut(s) 44
Bsh1236I CGCG 2 cut(s) 285, 785
BshFI GGCC 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 482
BsiSI CCGG 1 cut(s) 595
BslFI GGGAC 2 cut(s) 212, 704
BslI CCNNNNNNNGG 2 cut(s) 134, 418
BsmAI GTCTC 1 cut(s) 631
BsmFI GGGAC 2 cut(s) 212, 704
BsnI GGCC 1 cut(s) 234
Bso31I GGTCTC 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 482
Bsp143I GATC 1 cut(s) 539
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 2 cut(s) 746, 783
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 757
BspFNI CGCG 2 cut(s) 285, 785
BspLI GGNNCC 2 cut(s) 692, 693
BspTNI GGTCTC 1 cut(s) 631
BsrBI CCGCTC 1 cut(s) 748
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 1 cut(s) 539
BssNAI GTATAC 1 cut(s) 374
BssSI CACGAG 1 cut(s) 585
Bst1107I GTATAC 1 cut(s) 374
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 2 cut(s) 60, 165
Bst4CI ACNGT 2 cut(s) 91, 421
Bst6I CTCTTC 2 cut(s) 362, 573
BstAPI GCANNNNNTGC 2 cut(s) 266, 332
BstBAI YACGTR 1 cut(s) 548
BstDEI CTNAG 4 cut(s) 172, 184, 381, 765
BstEII GGTNACC 1 cut(s) 344
BstENI CCTNNNNNAGG 1 cut(s) 132
BstF5I GGATG 2 cut(s) 25, 124
BstFNI CGCG 2 cut(s) 285, 785
BstKTI GATC 1 cut(s) 542
BstMAI GTCTC 1 cut(s) 631
BstMBI GATC 1 cut(s) 539
BstMWI GCNNNNNNNGC 3 cut(s) 266, 332, 775
BstNI CCWGG 2 cut(s) 60, 165
BstNSI RCATGY 1 cut(s) 231
BstPI GGTNACC 1 cut(s) 344
BstSCI CCNGG 2 cut(s) 58, 163
BstSLI GKGCMC 1 cut(s) 482
BstUI CGCG 2 cut(s) 285, 785
BstV1I GCAGC 1 cut(s) 442
BstZ17I GTATAC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 234
BtsCI GGATG 2 cut(s) 25, 124
Cfr13I GGNCC 1 cut(s) 691
CviAII CATG 3 cut(s) 228, 413, 724
DdeI CTNAG 4 cut(s) 172, 184, 381, 765
DpnI GATC 1 cut(s) 541
DpnII GATC 1 cut(s) 539
Eam1104I CTCTTC 2 cut(s) 362, 573
EarI CTCTTC 2 cut(s) 362, 573
Eco31I GGTCTC 1 cut(s) 631
Eco32I GATATC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 691
Eco57I CTGAAG 1 cut(s) 774
Eco91I GGTNACC 1 cut(s) 344
EcoNI CCTNNNNNAGG 1 cut(s) 132
EcoO109I RGGNCCY 1 cut(s) 691
EcoO65I GGTNACC 1 cut(s) 344
EcoRII CCWGG 2 cut(s) 58, 163
EcoRV GATATC 1 cut(s) 600
FaeI CATG 3 cut(s) 231, 416, 727
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 2 cut(s) 212, 704
FatI CATG 3 cut(s) 227, 412, 723
FblI GTMKAC 2 cut(s) 160, 373
Fnu4HI GCNGC 1 cut(s) 456
FokI GGATG 2 cut(s) 32, 111
Fsp4HI GCNGC 1 cut(s) 456
FspBI CTAG 2 cut(s) 359, 699
GluI GCNGC 1 cut(s) 456
GsaI CCCAGC 1 cut(s) 48
GsuI CTGGAG 1 cut(s) 791
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 595
Hin1II CATG 3 cut(s) 231, 416, 727
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 2 cut(s) 203, 383
HinfI GANTC 2 cut(s) 461, 563
HpaII CCGG 1 cut(s) 595
HphI GGTGA 2 cut(s) 88, 356
Hpy166II GTNNAC 4 cut(s) 161, 374, 480, 730
Hpy188III TCNNGA 5 cut(s) 104, 128, 359, 493, 587
Hpy8I GTNNAC 4 cut(s) 161, 374, 480, 730
HpyAV CCTTC 2 cut(s) 383, 545
HpyCH4III ACNGT 2 cut(s) 91, 421
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 8 cut(s) 4, 99, 134, 227, 260, 335, 480, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 332, 775
HpyF3I CTNAG 4 cut(s) 172, 184, 381, 765
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 3 cut(s) 231, 416, 727
KflI GGGWCCC 1 cut(s) 691
Kzo9I GATC 1 cut(s) 539
LmnI GCTCC 2 cut(s) 137, 753
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 3 cut(s) 121, 335, 427
MaeI CTAG 2 cut(s) 359, 699
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 4 cut(s) 76, 298, 344, 632
MalI GATC 1 cut(s) 541
MbiI CCGCTC 1 cut(s) 748
MboI GATC 1 cut(s) 539
MboII GAAGA 3 cut(s) 290, 379, 560
MhlI GDGCHC 1 cut(s) 482
MluCI AATT 3 cut(s) 293, 429, 612
MluI ACGCGT 1 cut(s) 283
MnlI CCTC 7 cut(s) 29, 100, 418, 500, 520, 637, 705
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 3 cut(s) 207, 254, 354
MslI CAYNNNNRTG 2 cut(s) 129, 685
MspI CCGG 1 cut(s) 595
MspR9I CCNGG 2 cut(s) 60, 165
MvaI CCWGG 2 cut(s) 60, 165
MvnI CGCG 2 cut(s) 285, 785
MwoI GCNNNNNNNGC 3 cut(s) 266, 332, 775
NdeII GATC 1 cut(s) 539
NlaIII CATG 3 cut(s) 231, 416, 727
NlaIV GGNNCC 2 cut(s) 692, 693
NmuCI GTSAC 4 cut(s) 76, 298, 344, 632
NspI RCATGY 1 cut(s) 231
OliI CACNNNNGTG 1 cut(s) 685
PcsI WCGNNNNNNNCGW 1 cut(s) 544
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 2 cut(s) 461, 563
PkrI GCNGC 1 cut(s) 457
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 691
PsiI TTATAA 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 2 cut(s) 58, 163
PspEI GGTNACC 1 cut(s) 344
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 2 cut(s) 58, 163
PspN4I GGNNCC 2 cut(s) 692, 693
PspPI GGNCC 1 cut(s) 691
PspPPI RGGWCCY 1 cut(s) 691
RseI CAYNNNNRTG 2 cut(s) 129, 685
SaqAI TTAA 3 cut(s) 207, 254, 354
SatI GCNGC 1 cut(s) 456
Sau3AI GATC 1 cut(s) 539
Sau96I GGNCC 1 cut(s) 691
ScrFI CCNGG 2 cut(s) 60, 165
SduI GDGCHC 1 cut(s) 482
SfaNI GCATC 3 cut(s) 121, 335, 427
SinI GGWCC 1 cut(s) 691
SmiMI CAYNNNNRTG 2 cut(s) 129, 685
SmlI CTYRAG 2 cut(s) 491, 628
SmoI CTYRAG 2 cut(s) 491, 628
Sse9I AATT 3 cut(s) 293, 429, 612
SsiI CCGC 2 cut(s) 746, 783
SspMI CTAG 2 cut(s) 359, 699
StyD4I CCNGG 2 cut(s) 58, 163
TaaI ACNGT 2 cut(s) 91, 421
TaiI ACGT 1 cut(s) 550
TasI AATT 3 cut(s) 293, 429, 612
TfiI GAWTC 2 cut(s) 461, 563
Tru1I TTAA 3 cut(s) 207, 254, 354
Tru9I TTAA 3 cut(s) 207, 254, 354
TseFI GTSAC 4 cut(s) 76, 298, 344, 632
TseI GCWGC 1 cut(s) 455
Tsp45I GTSAC 4 cut(s) 76, 298, 344, 632
TspDTI ATGAA 4 cut(s) 132, 321, 486, 560
VneI GTGCAC 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 691
XagI CCTNNNNNAGG 1 cut(s) 132
XapI RAATTY 1 cut(s) 612
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 1 cut(s) 231
XmiI GTMKAC 2 cut(s) 160, 373
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 2 cut(s) 359, 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.